Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	2,089,794	T→C	intergenic (‑190051/–)	unknown ← / –	tRNA‑Met‑CAT/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	2,089,794	0	T	C	100.0%	218.2 / NA	73	intergenic (‑190051/–)	unknown/–	tRNA‑Met‑CAT/–
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (42/31);  total (42/31)

GTGATAGAACAGGACAGACAACAACACCATTTGCTGGAACAGGAAGGCTTCCGCTTAAATCCGAAGAAATCGGTGTTTGAATTGAATTTGGCAGGTTGATGAGAAAAAGTGCGGTGGACATTTGCCGTGAATTTCCACCGCACTTTTGTTGGCGTAAGGGCGAACCTACATCTTAAATAATCCGCGAAAACTGTTGTTGTTTTGCTTGTGCGCGGGAATACGTGTCGAAATACAAACAAATATTGCGAATCAACAAACGCCCTTTGGCGGAAACGGTGATTTGTGCTTCGCCGATGTCCAGCAAACCGTCGGCGGCTAAAGGCGCCAGCAGTGCTAAATCCTCGGCGAAATATTGTTTAAAATGAATATGATATTGTTGTTCAATCGGCGCGTAAGCGAGTTTAAAGTTACAAATCAATTGTTTAATCACATCACGACGCAGACAATCTTCTTTGGTCATGGCTAAGCCTTTATGC  >  1/2089564‑2090039                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      gTGATAGAACAGGACAGACAACAACACCATTTGCTGGAACAGGAAGGCTTCCGCTTAAATCCGAAGAAATCGGTGTTTGAATTGAATTTGGCAGGTTGATGAGAAAAAGTGCGGTGGACATTTGCCGTGAATTTCCACCGCACTTTTGTTGGCGTAAGGGCGAACCTACATCTTAAATAATCCGCGAAAACTGTTGTTGTTTTGCTTGTGCGCGGGAATACGTGTCGAAACACAAACAAATATTGCGAAt                                                                                                                                                                                                                                    >  1:745475/1‑250 (MQ=255) gTGATAGAACAGGACAGACAACAACACCATTTGCTGGAACAGGAAGGCTTCCGCTTAAATCCGAAGAAATCGGTGTTTGAATTGAATTTGGCAGGTTGATGAGAAAAAGTGCGGTGGACATTTGCCGTGAATTTCCACCGCACTTTTGTTGGCGTAAGGGCGAACCTACATCTTAAATAATCCGCGAAAACTGTTGTTGTTTTGCTTGTGCGCGGGAATACGTGTCGAAACACAAACAAATATTGCGAAt                                                                                                                                                                                                                                    >  1:168208/1‑250 (MQ=255)  tGATAGAACAGGACAGACAACAACACCATTTGCTGGAACAGGAAGGCTTCCGCTTAAATCCGAAGAAATCGGTGTTTGAATTGAATTTGGCAGGTTGATGAGAAAAAGTGCGGTGGACATTTGCCGTGAATTTCCACCGCACTTTTGTTGGCGTAAGGGCGAACCTACATCTTAAATAATCCGCGAAAACTGTTGTTGTTTTGCTTGTGCGCGGGAATACGTGTCGAAACACAAACAAATATTGCGAATc                                                                                                                                                                                                                                   <  1:514358/250‑1 (MQ=255)             gacagacaACAACACCATTTGCTGGAACAGGAAGGCTTCCGCTTAAATCCGAAGAAATCGGTGTTTGAATTGAATTTGGCAGGTTGATGAGAAAAAGTGCGGTGGACATTTGCCGTGAATTTCCACCGCACTTTTGTTGGCGTAAGGGCGAACCTACATCTTAAATAATCCGCGAAAACTGTTGTTGTTTTGCTTGTGCGCGGGAATACGTGTCGAAACACAAACAAATATTGCGAATcaacaacgc                                                                                                                                                                                                                           <  1:40986/247‑4 (MQ=255)                 gacaACAACACCATTTGCTGGAACAGGAAGGCTTCCGCTTAAATCCGAAGAAATCGGTGTTTGAATTGAATTTGGCAGGTTGATGAGAAAAAGTGCGGTGGACATTTGCCGTGAATTTCCACCGCACTTTTGTTGGCGTAAGGGCGAACCTACATCTTAAATAATCCGCGAAAACTGTTGTTGTTTTGCTTGTGCGCGGGAATACGTGTCGAAACACAAACAAATATTGCGAATCAACAAACGCCCTTTgg                                                                                                                                                                                                                   >  1:334478/1‑251 (MQ=255)                 gacaACAACACCATTTGCTGGAACAGGAAGGCTTCCGCTTAAATCCGAAGAAATCGGTGTTTGAATTGAATTTGGCAGGTTGATGAGAAAAAGTGCGGTGGACATTTGCCGTGAATTTCCACCGCACTTTTGTTGGCGTAAGGGCGAACCTACATCTTAAATAATCCGCGAAAACTGTTGTTGTTTTGCTTGTGCGCGGGAATACGTGTCGAAACACAAACAAATATTGCGAATCAACAAACGCCCTTTgg                                                                                                                                                                                                                   >  1:314691/1‑251 (MQ=255)                  acaacaacaCCATTTGCTGGAACAGGAAGGCTTCCGCTTAAATCCGAAGAAATCGGTGTTTGAATTGAATTTGGCAGGTTGATGAGAAAAAGTGCGGTGGACATTTGCCGTGAATTTCCACCGCACTTTTGTTGGCGTAAGGGCGAACCTACATCTTAAATAATCCGCGAAAACTGTTGTTGTTTTGCTTGTGCGCGGGAATACGTGTCGAAACACAAACAAATATTGCGAATCAACAAACGCCCTTTGGc                                                                                                                                                                                                                  <  1:171034/251‑1 (MQ=255)                  acaacaacaCCATTTGCTGGAACAGGAAGGCTTCCGCTTAAATCCGAAGAAATCGGTGTTTGAATTGAATTTGGCAGGTTGATGAGAAAAAGTGCGGTGGACATTTGCCGTGAATTTCCACCGCACTTTTGTTGGCGTAAGGGCGAACCTACATCTTAAATAATCCGCGAAAACTGTTGTTGTTTTGCTTGTGCGCGGGAATACGTGTCGAAACACAAACAAATATTGCGAATCAACAAACGCCCTTTGGc                                                                                                                                                                                                                  <  1:608947/251‑1 (MQ=255)                   taacaacaCCATTTGCTGGAACAGGAAGGCTTCCGCTTAAATCCGAAGAAATCGGTGTTTGAATTGAATTTGGCAGGTTGATGAGAAAAAGTGCGGTGGACATTTGCCGTGAATTTCCACCGCACTTTTGTTGGCGTAAGGGCGAACCTACATCTTAAATAATCCGCGAAAACTGTTGTTGTTTTGCTTGTGCGCGGGAATACGTGTCGAAACACAAACAAATATTGCGAATCAACAAACGCCCTTTGGCg                                                                                                                                                                                                                 >  1:744302/2‑251 (MQ=255)                   caacaacaCCATTTGCTGGAACAGGAAGGCTTCCGCTTAAATCCGAAGAAATCGGTGTTTGAATTGAATTTGGCAGGTTGATGAGAAAAAGTGCGGTGGACATTTGCCGTGAATTTCCACCGCACTTTTGTTGGCGTAAGGGCGAACCTACATCTTAAGTAATCCGCGAAAACTGTTGTTGTTTTGCTTGTGCGCGGGAATACGTGTCGAAACACAAACAAATATTGCGAATCAACAAACGCCCTTTGGCg                                                                                                                                                                                                                 >  1:782936/1‑251 (MQ=255)                   caacaacaCCATTTGCTGGAACAGGAAGGCTTCCGCTTAAATCCGAAGAAATCGGTGTTTGAATTGAATTTGGCAGGTTGATGAGAAAAAGTGCGGTGGACATTTGCCGTGAATTTCCACCGCACTTTTGTTGGCGTAAGGGCGAACCTACATCTTAAATAATCCGCGAAAACTGTTGTTGTTTTGCTTGTGCGCGGGAATACGTGTCGAAACACAAACAAat                                                                                                                                                                                                                                             >  1:420385/1‑223 (MQ=255)                   caacaacaCCATTTGCTGGAACAGGAAGGCTTCCGCTTAAATCCGAAGAAATCGGTGTTTGAATTGAATTTGGCAGGTTGATGAGAAAAAGTGCGGTGGACATTTGCCGTGAATTTCCACCGCACTTTTGTTGGCGTAAGGGCGAACCTACATCTTAAATAATCCGCGAAAACTGTTGTTGTTTTGCTTGTGCGCGGGAATACGTGTCGAAACACAAACAAATATTGCGAATCAACAAACGCCCTTTGGCg                                                                                                                                                                                                                 >  1:744310/1‑251 (MQ=255)                   caacaacaCCATTTGCTGGAACAGGAAGGCTTCCGCTTAAATCCGAAGAAATCGGTGTTTGAATTGAATTTGGCAGGTTGATGAGAAAAAGTGCGGCGGACATTTGCCGTGAATTTCCACCGCACTTTTGTTGGCGTAAGGGCGAACCTACATCTTAAATAATCCGCGAAAACTGTTGTTGTTTTGCTTGTGCGCGGGAATACGTGTCGAAACACAAACAAATATTGCGAATCAACAAACGCCCTTTGGCg                                                                                                                                                                                                                 >  1:687552/1‑251 (MQ=255)                                     gAACAGGAAGGCTTCCGCTTAAATCCGAAGAAATCGGTGTTTGAATTGAATTTGGCAGGTTGATGAGAAAAAGTGCGGTGGACATTTGCCGTGAATTTCCACCGCACTTTTGTTGGCGTAAGGGCGAACCTACATCTTAAATAATCCGCGAAAACTGTTGTTGTTTTGCTTGTGCGCGGGAATACGTGTCGAAACACAAACAAATATTGCGAATCAACAAACGCCCTTTGGCGGAAACGGTGATTtgtg                                                                                                                                                                                                 >  1:526467/1‑249 (MQ=255)                                      aaCAGGAAGGCTTCCGCTTAAATCCGAAGAAATCGGTGTTTGAATTGAATTTGGCAGGTTGATGAGAAAAAGTGCGGTGGACATTTGCCGTGAATTTCCACCGCACTTTTGTTGGCGTAAGGGCGAACCTACATCTTAAATAATCCGCGAAAACTGTTGTTGTTTTGCTTGTGCGCGGGAATACGTGTCGAAACACAAACAAATATTGCGAATCAACAAACGCCCTTTGGCGGAAACGGTGATTTGTGCtt                                                                                                                                                                                              >  1:362125/1‑251 (MQ=255)                                        cAGGAAGGCTTCCGCTTAAATCCGAAGAAATCGGTGTTTGAATTGAATTTGGCAGGTTGATGAGAAAAAGTGCGGTGGACATTTGCCGTGAATTTCCACCGCACTTTTGTTGGCGTAAGGGCGAACCTACATCTTAAATAATCCGCGAAAACTGTTGTTGTTTTGCTTGTGCGCGGGAATACGTGTCGAAACACAAACAAATATTGCGAATCAACAAACGCCCTTTGGCGGAAACGGTGATTTGTGc                                                                                                                                                                                                <  1:642161/247‑1 (MQ=255)                                        cAGGAAGGCTTCCGCTTAAATCCGAAGAAATCGGTGTTTGAATTGAATTTGGCAGGTTGATGAGAAAAAGTGCGGTGGACATTTGCCGTGAATTTCCACCGCACTTTTGTTGGCGTAAGGGCGAACCTACATCTTAAATAATCCGCGAAAACTGTTGTTGTTTTGCTTGTGCGCGGGAATACGTGTCGAAACACAAACAAATATTGCGAATCAACAAACGCCCTTTGGCGGAAACGGTGATTTGTGCTTCg                                                                                                                                                                                            >  1:472901/1‑251 (MQ=255)                                                      cTTAAATCCGAAGAAATCGGTGTTTGAATTGAATTTGGCAGGTTGATGAGAAAAAGTGCGGTGGACATTTGCCGTGAATTTCCACCGCACTTTTGTTGGCGTAAGGGCGAACCTACATCTTAAATAATCCGCGAAAACTGTTGTTGTTTTGCTTGTGCGCGGGAATACGTGTCGAAACACAAACAAATATTGCGAATCAACAAACGCCCTTTGGCGGAAACGGTGATTTGTGCTTCGCCGATGTCCAGCaa                                                                                                                                                                              <  1:23933/251‑1 (MQ=255)                                                      cTTAAATCCGAAGAAATCGGTGTTTGAATTGAATTTGGCAGGTTGATGAGAAAAAGTGCGGTGGACATTTGCCGTGAATTTCCACCGCACTTTTGTTGGCGTAAGGGCGAACCTACATCTTAAATAATCCGCGAAAACTGTTGTTGTTTTGCTTGTGCGCGGGAATACGTGTCGAAACACAAACAAATATTGCGAATCAACAAACGCCCTTTGGCGGAAACGGTGATTTGTGCTTCGCCGATGTCCAGCaa                                                                                                                                                                              >  1:345089/1‑251 (MQ=255)                                                        tAAATCCGAAGAAATCGGTGTTTGAATTGAATTTGGCAGGTTGATGAGAAAAAGTGCGGTGGACATTTGCCGTGAATTTCCACCGCACTTTTGTTGGCGTAAGGGCGAACCTACATCTTAAATAATCCGCGAAAACTGTTGTTGTTTTGCTTGTGCGCGGGAATACGTGTCGAAACACAAACAAATATTGCGAATCAACAAACGCCCTTTGGCGGAAACGGTGATTTGTGCTTCGCCGATGTCCAGCAAAc                                                                                                                                                                            <  1:170076/251‑1 (MQ=255)                                                         aaaTCCGAAGAAATCGGTGTTTGAATTGAATTTGGCAGGTTGATGAGAAAAAGTGCGGTGGACATTTGCCGTGAATTTCCACCGCACTTTTGTTGGCGTAAGGGCGAACCTACATCTTAAATAATCCGCGAAAACTGTTGTTGTTTTGCTTGTGCGCGGGAATACGTGTCGAAACACAAACAAATATTGCGAATCAACAAACGCCCTTTGGCGGAAACGGTGATTTGTGCTTCGCCGATGTCCAGCAAAcc                                                                                                                                                                           <  1:322039/251‑1 (MQ=255)                                                          aaTCCGAAGAAATCGGTGTTTGAATTGAATTTGGCAGGTTGATGAGAAAAAGTGCGGTGGACATTTGCCGTGAATTTCCACCGCACTTTTGTTGGCGTAAGGGCGAACCTACATCTTAAATAATCCGCGAAAACTGTTGTTGTTTTGCTTGTGCGCGGGAATACGTGTCGAAACACAAACAAATATTGCGAATCAACAAACGCCCTTTGGCGGAAACGGTGATTTGTGCTTCGCCGATGTCCAGCAAACCg                                                                                                                                                                          >  1:687619/1‑251 (MQ=255)                                                                         gtgtTTGAATTGAATTTGGCAGGTTGATGAGAAAAAGTGCGGTGGACATTTGCCGTGAATTTCCACCGCACTTTTGTTGGCGTAAGGGCGAACCTACATCTTAAATAATCCGCGAAAACTGTTGTTGTTTTGCTTGTGCGCGGGAATACGTGTCGAAACACAAACAAATATTGCGAATCAACAAACGCCCTTTGGCGGAAACGGTGATTTGTGCTTCGCCGATGTCCAGCAAACCGTCGGCGGCTAAAgg                                                                                                                                                            >  1:754831/1‑250 (MQ=255)                                                                                           gCAGGTTGATGAGAAAAAGTGCGGTGGACATTTGCCGTGAATTTCCACCGCACTTTTGTTGGCGTAAGGGCGAACCTACATCTTAAATAATCCGCGAAAACTGTTGTTGTTTTGCTTGTGCGCGGGAATACGTGTCGAAACACAAACAAATATTGCGAATCAACAAACGCCCTTTGGCGGAAACGGTGATTTGTGCTTCGCCGATGTCCAGCAAACCGTCGGCGGCTAAAGGCGCCAGCAGTGCTAAATc                                                                                                                                          <  1:518221/250‑1 (MQ=255)                                                                                               gTTGATGAGAAAAAGTGCGGTGGACATTTGCCGTGAATTTCCACCGCACTTTTGTTGGCGTAAGGGCGAACCTACATCTTAAATAATCCGCGAAAACTGTTGTTGTTTTGCTTGTGCGCGGGAATACGTGTCGAAACACAAACAAATATTGCGAATCAACAAACGCCCTTTGGCGGAAACGGTGATTTGTGCTTCGCCGATGTCCAGCAAACCGTcggc                                                                                                                                                                     <  1:860538/219‑1 (MQ=255)                                                                                                tacgccaacaaaaGTGCGGTGGACATTTGCCGTGAATTTCCACCGCACTTTTGTTGGCGTAAGGGCGAACCTACATCTTAAATAATCCGCGAAAACTGTTGTTGTTTTGCTTGTGCGCGGGAATACGTGTCGAAACACAAACAAATATTGCGAATCAACAAACGCCCTTTGGCGGAAACGGTGATTTGTGCTTCGCCGATGTCCAGCAAACCGTCGGCGGCTAAAGGCGCCAGCAGTGCTAAATCCTCgg                                                                                                                                     >  1:192333/10‑250 (MQ=255)                                                                                                  gatgaGAAAAAGTGCGGTGGACATTTGCCGTGAATTTCCACCGCACTTTTGTTGGCGTAAGGGCGAACCTACATCTTAAATAATCCGCGAAAACTGTTGTTGTTTTGCTTGTGCGCGGGAATACGTGTCGAAACACAAACAAATATTGCGAATCAACAAACGCCCTTTGGCGGAAACGGTGATTTGTGCTTCGCCGATGTCCAGCAAACCGTCGGCGGCTAAAGGCGCCAGCAGTGCTAAATc                                                                                                                                          <  1:34654/243‑1 (MQ=255)                                                                                                             gTGCGGTGGACATTTGCCGTGAATTTCCACCGCACTTTTGTTGGCGTAAGGGCGAACCTACATCTTAAATAATCCGCGAAAACTGTTGTTGTTTTGCTTGTGCGCGGGAATACGTGTCGAAACACAAACAAATATTGCGAATCAACAAACGCCCTTTGGCGGAAACGGTGATTTGTGCTTCGCCGATGTCCAGCAAACCGTCGGCGGCTAAAGGCGCCAGCAGTGCTAAAt                                                                                                                                           >  1:142755/1‑231 (MQ=255)                                                                                                                  gTGGACATTTGCCGTGAATTTCCACCGCACTTTTGTTGGCGTAAGGGCGAACCTACATCTTAAATAATCCGCGAAAACTGTTGTTGTTTTGCTTGTGCGCGGGAATACGTGTCGAAACACAAACAAATATTGCGAATCAACAAACGCCCTTTGGCGGAAACGGTGATTTGTGCTTCGCCGATGTCCAGCAAACCGTCGGCGGCTAAAGGCGCCAGCAGTGCTAAATCCTCGGCGAAatat                                                                                                                             <  1:718266/240‑1 (MQ=255)                                                                                                                    ggACATTTGCCGTGAATTTCCACCGCACTTTTGTTGGCGTAAGGGCGAACCTACATCTTAAATAATCCGCGAAAACTGTTGTTGTTTTGCTTGTGCGCGGGAATACGTGTCGAAACACAAACAAATATTGCGAATCAACAAACGCCCTTTGGCGGAAACGGTGATTTGTGCTTCGCCGATGTCCAGCAAACCg                                                                                                                                                                          <  1:466654/193‑1 (MQ=255)                                                                                                                    ggACATTTGCCGTGAATTTCCACCGCACTTTTGTTGGCGTAAGGGCGAACCTACATCTTAAATAATCCGCGAAAACTGTTGTTGTTTTGCTTGTGCGCGGGAATACGTGTCGAAACACAAACAAATATTGCGAATCAACAAACGCCCTTTGGCGGAAACGGTGATTTGTGCTTCGCCGATGTCCAGCAAACCGTCGGCGGCTAAAGGCGCCAGCAGTGCTa                                                                                                                                              <  1:277977/221‑1 (MQ=255)                                                                                                                      aCATTTGCCGTGAATTTCCACCGCACTTTTGTTGGCGTAAGGGCGAACCTACATCTTAAATAATCCGCGAAAACTGTTGTTGTTTTGCTTGTGCGCGGGAATACGTGTCGAAACACAAACAAATATTGCGAATCAACAAACGCCCTTTGGCGGAAACGGTGATTTGTGCTTCGCCGATGTCCAGCAAACCGTCGGCGGCTAAAGGCGCCAGCAGTGCTAAATCCTCGGCGAAATATTGTTTAAAATGAat                                                                                                               <  1:386258/250‑1 (MQ=255)                                                                                                                          ttGCCGTGAATTTCCACCGCACTTTTGTTGGCGTAAGGGCGAACCTACATCTTAAATAATCCGCGAAAACTGTTGTTGTTTCGCTTGTGCGCGGGAATACGTGTCGAAACACAAACAAATATTGCGAATCAACAAACGCCCTTTGGCGGAAACGGTGATTTGTGCTTCGCCGATGTCCAGCAAACCGTCGGCGGCTAAAGGCGCCAGCAGTGCTAAATCCTCGGCGAAATATTGTTTAAAATGAATATGat                                                                                                          <  1:449121/251‑1 (MQ=255)                                                                                                                                tGAATTTCCACCGCACTTTTGTTGGCGTAAGGGCGAACCTACATCTTAAATAATCCGCGAAAACTGTTGTTGTTTTGCTTGTGCGCGGGAATACGTGTCGAAACACAAACAAATATTGCGAATCAACAAACGCCCTTTGGCGGAAACGGTGATTTGTGCTTCGCCGATGTCCAGCAAACCGTCGGCGGCTAAAGGCGCCAGCAGTGCTAAATCCTCGGCGAAATATTGTTTAAAATGAATATGATAttgt                                                                                                     <  1:867048/250‑1 (MQ=255)                                                                                                                                  aaTTTCCACCGCACTTTTGTTGGCGTAAGGGCGAACCTACATCTTAAATAATCCGCGAAAACTGTTGTTGTTTTGCTTGTGCGCGGGAATACGTGTCGAAACACAAACAAATATTGCGAATCAACAAACGCCCTTTGGCGGAAACGGTGATTTGTGCTTCGCCGATGTCCAGCAAACCGTCGGCGGCTAAAGGCGCCAGCAGTGCTAAATCCTCGGCGAAATATTGTTTAAAATGAATATGATAttgttg                                                                                                   <  1:489315/250‑1 (MQ=255)                                                                                                                                    tttCCACCGCACTTTTGTTGGCGTAAGGGCGAACCTACATCTTAAATAATCCGCGAAAACTGTTGTTGTTTTGCTTGTGCGCGGGAATACGTGTCGAAACACAAACAAATATTGCGAATCAACAAACGCCCTTTGGCGGAAACGGTGATTTGTGCTTCGCCGATGTCCAGCAAACCGTCGGCGGCTAAAGGCGCCAGCAGTGCTAAATCCTCGGCGAAATATTGTTTAAAATGAATATGatat                                                                                                        >  1:501698/1‑243 (MQ=255)                                                                                                                                       ccACCGCACTTTTGTTGGCGTAAGGGCGAACCTACATCTTAAATAATCCGCGAAAACTGTTGTTGTTTTGCTTGTGCGCGGGAATACGTGTCGAAACACAAACAAATATTGCGAATCAACAAACGCCCTTTGGCGGAAACGGTGATTTGTGCTTCGCCGATGTCCAGCAAACCGTCGGCGGCTAAAGGCGCCAGCAGTGCTAAATCCTCGGCGAAATATTGTTTAAAATGAATATGATATTGTTGTTCAAt                                                                                             >  1:500376/1‑251 (MQ=255)                                                                                                                                            gCACTTTTGTTGGCGTAAGGGCGAACCTACATCTTAAATAATCCGCGAAAACTGTTGTTGTTTTGCTTGTGCGCGGGAATACGTGTCGAAACACAAACAAATATTGCGAATCAACAAACGCCCTTTGGCGGAAACGGTGATTTGTGCTTCGCCGATGTCCAGCAAACCGTCGGCGGCTAAAGGCGCCAGCAGTGCTAAATc                                                                                                                                          >  1:310375/1‑201 (MQ=255)                                                                                                                                            gCACTTTTGTTGGCGTAAGGGCGAACCTACATCTTAAATAATCCGCGAAAACTGTTGTTGTTTTGCTTGTGCGCGGGAATACGTGTCGAAACACAAACAAATATTGCGAATCAACAAACGCCCTTTGGCGGAAACGGTGATTTGTGCTTCGCCGATGTCCAGCAAACCGTCGGCGGCTAAAGGCGCCAGCAGTGCTAAATCCTCGGCGAAATATTGTTTAAAATGAATATGat                                                                                                          >  1:186734/1‑233 (MQ=255)                                                                                                                                               cTTTTGTTGGCGTAAGGGCGAACCTACATCTTAAATAATCCGCGAAAACTGTTGTTGTTTTGCTTGTGCGCGGGAATACGTGTCGAAACACAAACAAatat                                                                                                                                                                                                                                           >  1:690596/1‑101 (MQ=255)                                                                                                                                                  ttgttgGCGTAAGGGCGAACCTACATCTTAAATAATCCGCGAAAACTGTTGTTGTTTTGCTTGTGCGCGGGAATACGTGTCGAAACACAAACAAATATTGCGAATCAACAAACGCCCTTTGGCGGAAACGGTGATTTGTGCTTCGCCGATGTCCAg                                                                                                                                                                                 <  1:723165/156‑1 (MQ=255)                                                                                                                                                        gCGTAAGGGCGAACCTACATCTTAAATAATCCGCGAAAACTGTTGTTGTTTTGCTTGTGCGCGGGAATACGTGTCGAAACACAAACAAATATTGCGAATCAACAAACGCCCTTTGGCGGAAACGGTGATTTGTGCTTCGCCGATGTCCAGCaa                                                                                                                                                                              <  1:581356/153‑1 (MQ=255)                                                                                                                                                        gCGTAAGGGCGAACCTACATCTTAAATAATCCGCGAAAACTGTTGTTGTTTTGCTTGTGCGCGGGAATACGTGTCGAAACACAAACAAATATTGCGAATCAACAAACGCCCTTTGGCGGAAACGGTGATTTGTGCTTCGCCGATGTCCAGCAAACCGTCGGCGGCTAAAGGCGCCAGCAGTGCTAAATCCTCGGCGAAATATTGTTTAAAATGAATATGATATTGTTGTTCAATCGGCGCGTAAGCGAGtt                                                                            >  1:205943/1‑251 (MQ=255)                                                                                                                                                        gCGTAAGGGCGAACCTACATCTTAAATAATCCGCGAAAACTGTTGTTGTTTTGCTTGTGCGCGGGAATACGTGTCGAAACACAAACAAATATTGCGAATCAACAAACGCCCTTTGGCGGAAACGGTGATTTGTGCTTCGCCGATGTCCAGCAAACCGTCGGCGGCTAAAGGCGCCAGCAGTGCTAAATCCTCGGCGAAATATTGTTTAAAATGAATATGATATTGTTGTTCAATCGGCGCGTAAGCGAGtt                                                                            >  1:471404/1‑251 (MQ=255)                                                                                                                                                                   aaCCTACATCTTAAATAATCCGCGAAAACTGTTGTTGTTTTGCTTGTGCGCGGGAATACGTGTCGAAACACAAACAAATATTGCGAATCAACAAACGCCCTTTGGCGGAAACGGTGATTTGTGCTTCGCCGATGTCCAGCAAACCGTCGGCGGCTAAAGGCGCCAGCAGTGCTAAATCCTCGGCGAAATATTGTTTAAAATGAATATGATATTGTTGTTCAATCGGCGCGTAAg                                                                                  >  1:767012/1‑234 (MQ=255)                                                                                                                                                                     ccTACATCTTAAATAATCCGCGAAAACTGTTGTTGTTTTGCTTGTGCGCGGGAATACGTGTCGAAACACAAACAAATATTGCGAATcaac                                                                                                                                                                                                                                >  1:398257/1‑90 (MQ=255)                                                                                                                                                                     ccTACATCTTAAATAATCCGCGAAAACTGTTGTTGTTTTGCTTGTGCGCGGGAATACGTGTCGAAACACAAACAAATATTGCGAATCAACAAACGCCCTTTGGCGGAAACGGTGATTTGTGCTTCGCCGATGTCCAGCAAACCGTCGGCGGCTAAAGGCGCCAGCAGTGCTAAATCCTCGGCGAAATATTGTTTAAAATGAATATGATATTGTTGTTCAATCGGCGCGTAAGCGAGTTTAAAGTTACAAAt                                                               >  1:382464/1‑251 (MQ=255)                                                                                                                                                                      cTACATCTTAAATAATCCGCGAAAACTGTTGTTGTTTTGCTTGTGCGCGGGAATACGTGTCGAAACACAAACAAATATTGCGAATCAACAAACGCCCTTTGGCGGAAACGGTGATTTGTGCTTCGCCGATGTCCAGCAAACCGTCGGCGGCTAAAGGCGCCAGCAGTGCTAAATCCTCGGCGAAATATTGTTTAAAATGAATATGATATTGTTGTTCAATCGGCGCGTAAGCGAGTTTAAAGTTACAAAt                                                               >  1:566630/1‑250 (MQ=255)                                                                                                                                                                         cATCTTAAATAATCCGCGAAAACTGTTGTTGTTTTGCTTGTGCGCGGGAATACGTGTCGAAACACAAACAAATATTGCGAATCAACAAACGCCCTTTGGCGGAAACGGTGATTTGTGCTTCGCCGATGTCCAGCAAACCGTCGGCGGCTAAAGGCGCCAGCAGTGCTAAATCCTCGGCGAAATATTGTTTAAAATGAATATGATATTGTTGTTCAATCGGCGCGTAAGCGAGTTTAAAGTTACAAATCAAt                                                           >  1:415050/1‑251 (MQ=255)                                                                                                                                                                            cTTAAATAATCCGCGAAAACTGTTGTTGTTTTGCTTGTGCGCGGGAATACGTGTCGAAACACAAACAAATATTGCGAATCAACAAACGCCCTTTGGCGGAAACGGTGATTTGTGCTTCGCCGATGTCCAGCAAACCGTCGGCGGCTAAAGGCGCCAGCAGTGCTAAATCCTCGGCGAAATATTGTTTAAAATGAATATGATATTGTTGTTCAATCGGCGCGTAAGCg                                                                                >  1:756724/1‑227 (MQ=255)                                                                                                                                                                              tAAATAATCCGCGAAAACTGTTGTTGTTTTGCTTGTGCGCGGGAATACGTGTCGAAACACAAACAAATATTGCGAATCAACAAACGCCCTTTGGCGGAAACGGTGATTTGTGCTTCGCCGATGTCCAGCAAACCGTCGGCGGCTAAAGGCGCCAGCAGTGCTAAATCCTCGGCGAAATATTGTTTAAAATGAATATGATATTGTTGTTc                                                                                                >  1:346750/1‑209 (MQ=255)                                                                                                                                                                              tAAATAATCCGCGAAAACTGTTGTTGTTTTGCTTGTGCGCGGGAATACGTGTCGAAACACAAACAAATATTGCGAATCAACAAACGCCCTTTGGCGGAAACGGTGATTTGTGCTTCGCCGATGTCCAGCAAACCGTCGGCGGCTAAAGGCGCCAGCAGTGCTAAATCCTCGGCGAAATATTGTTTAAAATGAATATGATATTGTTGTTCAATCGGCGCGTAAGCGAGTTTAAAGTTACAAATCAATTGttt                                                      >  1:554044/1‑251 (MQ=255)                                                                                                                                                                              tAAATAATCCGCGAAAACTGTTGTTGTTTTGCTTGTGCGCGGGAATACGTGTCGAAACACAAACAAATATTGCGAATCAACAAACGCCCTTTGGCGGAAACGGTGATTTGTGCTTCGCCGATGTCCAGCAAACCGTCGGCGGCTAAAGGCGCCAGCAGTGCTAAATCCTCGGCGAAATATTGTTTAAAATGAATATGATATTGTTGTTCAATCGGCGCGTAAGCGAGTTTAAAGTTACAAATCAATTGttt                                                      <  1:485310/251‑1 (MQ=255)                                                                                                                                                                               aaaTAATCCGCGAAAACTGTTGTTGTTTTGCTTGTGCGCGGGAATACGTGTCGAAACACAAACAAATATTGCGAATCAACAAACGCCCTTTGGCGGAAACGGTGATTTGTGCTTCGCCGATGTCCAGCAAACCGTCGGCGGCTAAAGGCGCCAGCAGTGCTAAATCCTCGGCGAAATATTGTTTAAAATGAATATGat                                                                                                          >  1:405022/1‑198 (MQ=255)                                                                                                                                                                                   aatCCGCGAAAACTGTTGTTGTTTTGCTTGTGCGCGGGAATACGTGTCGAAACACAAACAAATATTGCGAATCAACAAACGCCCTTTGGCGGAAACGGTGATTTGTGCTTCGCCGATGTCCAGCAAACCGTCGGCGGCTAAAGGCGCCAGCAGTGCTAAATCCTCGGCGAAATATTGTTTAAAATGAATATGATATTGTTGTTCAATCGGCGCGTAAGCGAGTTTAAAGTTACAAATCAATTGTTTAatca                                                 <  1:295688/251‑1 (MQ=255)                                                                                                                                                                                               cTGTTGTTGTTTTGCTTGTGCGCGGGAATACGTGTCGAAACACAAACAAATATTGCGAATCAACAAACGCCCTTTGGCGGAAACGGTGATTTGTGCTTCGCCGATGTCCAGCAAACCGTCGGCGGCTAAAGGCGCCAGCAGTGCTAAATCCTCGGCGAAATATTGTTTAAAATGAATATGATATTGTTGTTCAATCGGCGCGTAAGCGAGTTTAAAGTTACAAATCAATTGTTTAATCACATCACGACGCa                                     >  1:9350/1‑251 (MQ=255)                                                                                                                                                                                                 gttgttgttTTGCTTGTGCGCGGGAATACGTGTCGAAACACAAACAAATATTGCGAATCAACAAACGCCCTTTGGCGGAAACGGTGATTTGTGCTTCGCCGATGTCCAGCAAACCGTCGGCGGCTAAAGGCGCCAGCAGTGCTAAATCCTCGGCGAAATATTGttt                                                                                                                        <  1:299354/166‑1 (MQ=255)                                                                                                                                                                                                      tgttTTGCTTGTGCGCGGGAATACGTGTCGAAACACAAACAAATATTGCGAATCAACAAACGCCCTTTGGCGGAAACGGTGATTTGTGCTTCGCCGATGTCCAGCAAACCGTCGGCGGCTAAAGGCGCCAGCAGTGCTAAATCCTCGGCGAAATATTGTTTAAAATGAATATGATATTGTTGTTCAATCGGCGCGTAAGCGAGTTTAAAGTTACAAATCAATTGTTTAATCACATCacgac                                        >  1:142012/1‑241 (MQ=255)                                                                                                                                                                                                      tgttTTGCTTGTGCGCGGGAATACGTGTCGAAACACAAACAAATATTGCGAATCAACAAACGCCCTTTGGCGGAAACGGTGATTTGTGCTTCGCCGATGTCCAGCAAACCGTCGGCGGCTAAAGGCGCCAGCAGTGCTAAATCCTCGGCGAAATATTGTTTAAAATGAATATGATATTGTTGTTCAATCGGCGCGTAAGCGAGTTTAAAGTTACAAATCAATTGTTTAATCACATCACGACGCAGACAAt                               >  1:417034/1‑250 (MQ=255)                                                                                                                                                                                                       gttTTGCTTGTGCGCGGGAATACGTGTCGAAACACAAACAAATATTGCGAATCAACAAACGCCCTTTGGCGGAAACGGTGATTTGTGCTTCGCCGATGTCCAGCAAACCGTCGGCGGCTAAAGGCGCCAGCAGTGCTAAATCCTCGGCGAAATATTGTTTAAAATGAATATGATATTGTTGTTCAATCGGCGCGTAAGCGAGTTTAAAGTTACAAATCAATTGTTTAATCACATCACGACGCAGACAAt                               >  1:260043/1‑249 (MQ=255)                                                                                                                                                                                                         tttGCTTGTGCGCGGGAATACGTGTCGAAACACAAACAAATATTGCGAATCAACAAACGCCCTTTGGCGGAAACGGTGATTTGTGCTTCGCCGATGTCCAGCAAACCGTCGGCGGCTAAAGGCGCCAGCAGTGCTAAATCCTCGGCGAAATATTGTTTAAAATGAATATGATATTGTTGTTCAATCGGCGCGTAAGCGAGTTTAAAGTTACAAATCAATTGTTTAATCACATCACGACGCAGACAAtcttc                           <  1:362681/251‑1 (MQ=255)                                                                                                                                                                                                            gCTTGTGCGCGGGAATACGTGTCGAAACACAAACAAATATTGCGAATCAACAAACGCCCTTTGGCGGAAACGGTGATTTGTGCTTCGCCGATGTCCAGCAAACCGTCGGCGGCTAAAGGCGCCAGCAGTGCTAAATCCTCGGCGAAATATTGTTTAAAATGAATATGATAttgt                                                                                                     <  1:227466/174‑1 (MQ=255)                                                                                                                                                                                                                gtgCGCGGGAATACGTGTCGAAACACAAACAAATATTGCGAATCAACAAACGCCCTTTGGCGGAAACGGTGATTTGTGCTTCGCCGATGTCCAGCAAACCGTCGGCGGCTAAAGGCGCCAGCAGTGCTAAATCCTCGGCGAAATATTGTTTAAAATGAATATGATATTGTTGTTCAATCGGCGCGTAAGCGAGTTTAAAGTTACAAATCAATTGTTTAATCACATCACGACGCAGACAATCTTCTTTGGt                     >  1:472000/1‑250 (MQ=255)                                                                                                                                                                                                                 tgCGCGGGAATACGTGTCGAAACACAAACAAATATTGCGAATCAACAAACGCCCTTTGGCGGAAACGGTGATTTGTGCTTCGCCGATGTCCAGCAAACCGTCGGCGGCTAAAGGCGCCAGCAGTGCTAAATCCTCGGCGAAATATTGTTTAAAATGAATATGATATTGTTGTTCAATCGGCGCGTAAGCGAGTTTAAAGTTACAAATCAATTGTTTAATCACATCACGACGCAGACAATCTTCTTTGGTc                    <  1:530403/250‑1 (MQ=255)                                                                                                                                                                                                                 tgCGCGGGAATACGTGTCGAAACACAAACAAATATTGCGAATCAACAAACGCCCTTTGGCGGAAACGGTGATTTGTGCTTCGCCGATGTCCAGCAAACCGTCGGCGGCTAAAGGCGCCAGCAGTGCTAAATCCTCGGCGAAATATTGTTTAAAATGAATATGATATTGTTGTTCAATCGGCGCGTAAGCGAGTTTAAAGTTACAAATCAATTGTTTAATCACATCACGACGCAGACAATCTTCTTTGGTc                    <  1:770521/250‑1 (MQ=255)                                                                                                                                                                                                                     cgGGAATACGTGTCGAAACACAAACAAATATTGCGAATCAACAAACGCCCTTTGGCGGAAACGGTGATTTGTGCTTCGCCGATGTCCAGCAAACCGTCGGCGGCTAAAGGCGCCAGCAGTGCTAAATCCTCGGCGAAATATTGTTTAAAATGAATATGATATTGTTGTTCAATCGGCGCGTAAGCGAGTTTAAAGTTACAAATCAATTGTTTAATCACATCACGACGCAGACAATCTTCTTTGGTCATgg                >  1:869450/1‑250 (MQ=255)                                                                                                                                                                                                                     cgGGAATACGTGTCGAAACACAAACAAATATTGCGAATCAACAAACGCCCTTTGGCGGAAACGGTGATTTGTGCTTCGCCGATGTCCAGCAAACCGTCGGCGGCTAAAGGCGCCAGCAGTGCTAAATCCTCGGCGAAATATTGTTTAAAATGAATATGATATTGTTGTTCAATCGGCGCGTAAGCGAGTTTAAAGTTACAAATCAATTGTTTAATCACATCACGACGCAGACAATCTTCTTTGGTCATGGc               <  1:106260/251‑1 (MQ=255)                                                                                                                                                                                                                      gggAATACGTGTCGAAACACAAACAAATATTGCGAATCAACAAACGCCCTTTGGCGGAAACGGTGATTTGTGCTTCGCCGATGTCCAGCAAACCGTCGGCGGCTAAAGGCGCCAGCAGTGCTAAATCCTCGGCGAAATATTGTTTAAAATGAATATGATATTGTTGTTCAATCGGCGCGTAAGCGAGTTTAAAGTTACAAATCAATTGTTTAATCACATCACGACGCAGACAATCTTCTTTGGTCATgg                >  1:501760/1‑249 (MQ=255)                                                                                                                                                                                                                       ggAATACGTGTCGAAACACAAACAAATATTGCGAATCAACAAACGCCCTTTGGCGGAAACGGTGATTTGTGCTTCGCCGATGTCCAGCAAACCGTCGGCGGCTAAAGGCGCCAGCAGTGCTAAATCCTCGGCGAAATATTGTTTAAAATGAATATGATATTGTTGTTCAATCGGCGCGTAAGCGAGTTTAAAGTTACAAAt                                                               <  1:55133/201‑1 (MQ=255)                                                                                                                                                                                                                       ggAATACGTGTCGAAACACAAACAAATATTGCGAATCAACAAACGCCCTTTGGCGGAAACGGTGATTTGTGCTTCGCCGATGTCCAGCAAACCGTCGGCGGCTAAAGGCGCCAGCAGTGCTAAATCCTCGGCGAAATATTGTTTAAAATGAATATGATATTGTTGTTCAATCGGCGCGTAAGCGAGTTTAAAGTTACAAATCAATTGTTTAATCACATCACGACGCAGACAATCTTCTTTGGTCATGGCTa             >  1:113987/1‑251 (MQ=255)                                                                                                                                                                                                                       ggAATACGTGTCGAAACACAAACAAATATTGCGAATCAACAAACGCCCTTTGGCGGAAACGGTGATTTGTGCTTCGCCGATGTCCAGCAAACCGTCGGCGGCTAAAGGCGCCAGCAGTGCTAAATCCTCGGCGAAATATTGTTTAAAATGAATATGATATTGTTGTTCAATCGGCGCGTAAGCGAGTTTAAAGTTACAAATCAATTGTTTAATCACATCACGACGCAGACAATCTTCTTTGGTCATGGCTa             >  1:702829/1‑251 (MQ=255)                                                                                                                                                                                                                       ggAATACGTGTCGAAACACAAACAAATATTGCGAATCAACAAACGCCCTTTGGCGGAAACGGTGATTTGTGCTTCGCCGACGTCCAGCAAACCGTCGGCGGCTAAAGGCGCCAGCAGTGCTAAATCCTCGGCGAAATATTGTTTAAAATGAATATGATATTGTTGTTCAATCGGCGCGTAAGCGAGTTTAAAGTTACAAATCAATTGTTTAATCGCATCACGACGCAGACAATCTTCTTTGGTCATGGCt              <  1:423715/250‑1 (MQ=255)                                                                                                                                                                                                                                  cGAAACACAAACAAATATTGCGAATCAACAAACGCCCTTTGGCGGAAACGGTGATTTGTGCTTCGCCGATGTCCAGCAAACCGTCGGCGGCTAAAGGCGCCAGCAGTGCTAAATCCTCGGCGAAATATTGTTTAAAATGAATATGATATTGTTGTTCAATCGGCGCGTAAGCGAGTTTAAAGTTACAAATCAATTGTTTAATCACATCACGACGCAGACAATCTTCTTTGGTCATGGCTAAGCCTTTATGc  <  1:881793/251‑1 (MQ=255)                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      GTGATAGAACAGGACAGACAACAACACCATTTGCTGGAACAGGAAGGCTTCCGCTTAAATCCGAAGAAATCGGTGTTTGAATTGAATTTGGCAGGTTGATGAGAAAAAGTGCGGTGGACATTTGCCGTGAATTTCCACCGCACTTTTGTTGGCGTAAGGGCGAACCTACATCTTAAATAATCCGCGAAAACTGTTGTTGTTTTGCTTGTGCGCGGGAATACGTGTCGAAATACAAACAAATATTGCGAATCAACAAACGCCCTTTGGCGGAAACGGTGATTTGTGCTTCGCCGATGTCCAGCAAACCGTCGGCGGCTAAAGGCGCCAGCAGTGCTAAATCCTCGGCGAAATATTGTTTAAAATGAATATGATATTGTTGTTCAATCGGCGCGTAAGCGAGTTTAAAGTTACAAATCAATTGTTTAATCACATCACGACGCAGACAATCTTCTTTGGTCATGGCTAAGCCTTTATGC  >  1/2089564‑2090039

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑