Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	2,057,820	G→A	intergenic (‑158077/–)	unknown ← / –	tRNA‑Met‑CAT/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	2,057,820	0	G	A	100.0%	162.8 / NA	58	intergenic (‑158077/–)	unknown/–	tRNA‑Met‑CAT/–
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (26/32);  total (26/32)

CGCCAACAGCTGTTAAAA‑TCATCGTCGCT‑AAGGC‑TAATGCCAACAAAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCAGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACCGGCGGCTAAAAT‑CCGCATGACCGCCGTGGTAATGCCCTGCGCACCGCCGATCATGAGACTCACGGTTTGCACTAAATCGGCACCGCCGACCAAACCGCCGATTAACGCCCCCGCCAGCATACCGTATGCCGGCGATACT‑‑TTTTTCAAAATCAGGAGGATCGCCACCGCAAGTGCAATGACCGCTCCCAACGCTGAGACTGTCGTCATATAATTCTCCTCTGTGATGAACCGTGTAAATAACACAAAGGAGATTATTCGCCCTTAATGCAAAAATTGCTTTATATAAAAATACAAAAATCTT  >  1/2057575‑2058008                                                                                                                                                                                                                                                                |                                                                                                                                                                                               cGCCAACAGCTGTTAAAA‑TCATCGTCGCT‑AAGGC‑TAATGCCAACAAAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACCGGCGGCTAAAAT‑CCGCATGACCGCCGTGGTAATGCCCTGCGCACCGCCGATCATGAGACTCACGGTTTGCACTAAATCGGCACCGCCGACCAAACCGCCGATTAACGCCCCCACCAg                                                                                                                                                                                            >  1:869328/1‑250 (MQ=255)    ccaactcctgtaaaaaccttcgtcgCA‑AAGGC‑TAATCCCACCAAGGCCC‑GGGCTCCCCCCAAC‑TTATGGGAATTGGTTC‑CCGCAA‑CCTTGTTGGC‑GCCCCCG‑AAACAATCCACCCCGCCCGCCACCACCCCGGCGGCTAAAAC‑CCGCATGCCCGCCGTGGTAATGCCCTGCGCACCGCCGATCATGAGACTCACGGTTTGCACTAAATCGGCACCGCCGACCAAACCGCCGATTAACGCCCCCACCAGCAt                                                                                                                                                                                         <  1:91509/232‑1 (MQ=255)     aaCAGCTTTAAAAA‑TCATCGCCGC‑‑AAGGCATAATGCAAACAAGGC‑G‑GGGGTTCCCCCCACTTTATGGGTAA‑GGTTTCCCGCAA‑TCGTGTGGGCGGCACGGA‑AAACAAT‑CAGCACGCCCGCCAACACCCGGGCGCCTAAAAT‑CCGCATGACCGCCGTGGTAATGCCCTGCGCACCGCCGATCATGAGACTCACGGTTTGCACAAAATCGGCACCGCCGACCAAACCGCCGATTAACGCCCCCACCAGCATAc                                                                                                                                                                                       <  1:574478/251‑1 (MQ=255)      aacggctttAAAACTCATCGTCGAA‑AGGGC‑TAATGCCAACAAAGCAC‑GGGCTCCCCCCACT‑TTATTGGAAATGGTTC‑CCGCAA‑TGGTGTGGGCGGCCCCGA‑AACCAAT‑CAGCCCGCCCGCCACCACACCGGCGGCTAAAAT‑CCGCATGACCGCCGTGGTAATGCCCTGCGCACCGCCGATCATGAGACTCACGGTTTGCACTAAATCGGCACCGCCGACCAAACCGCCGATTAACGCCCCCACCAGCATAc                                                                                                                                                                                       <  1:857085/244‑1 (MQ=255)       caacTGTTAAAA‑TCTTCGTC‑CTGAAGAC‑TAATGCCAAC‑AAGCCCGGGGCTTCCCCCAAG‑TT‑TTAGGAATTGTTT‑TCGAAATTGGTTTTGGGGGCACCGG‑AATAAAA‑CAGCACCCCCGCCAACACACCGGCGGCAAAAAC‑CCGCTTGACCGCCGTGGTAATGCCCTGCGCACCGCCGATCATGAGACTCACGGTTTGCACTAAATCGGCACCGCCGACCAAACCGCCGATTAACGCCCCCACCAGCATACCg                                                                                                                                                                                     <  1:230556/248‑1 (MQ=255)            gTTAAAA‑TCATCGTCGCT‑AAGGC‑TAATGCCAACAAAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACCGGCGGCTAAAAT‑CCGCATGACCGCCGTGGTAATGCCCTGCGCACCGCCGATCATGAGACTCACGGTTTGCACTAAATCGGCACCGCCGACCAAACCGCCGATTAACGCCCCCACCAGc                                                                                                                                                                                           >  1:34620/1‑240 (MQ=255)            gTTAAAA‑TCATCGTCGCT‑AAGGC‑TAATGCCAACAAAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACCGGCGGCTAAAAT‑CCGCATGACCGCCGTGGTAATGCCCTGCGCACCGCCGATCATGAGACTCACGGTTTGCACTAAATCGGCACCGCCGACCAAACCGCCGATTAACGCCCCCACCAGCATACCGt                                                                                                                                                                                    >  1:113706/1‑247 (MQ=255)            gTTAAAA‑TCATCGTCGCT‑AAGGC‑TAATGCCAACAAAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACCGGCGGCTAAAAT‑CCGCATGACCGCCGTGGTAATGCCCTGCGCACCGCCGATCATGAGACTCACGGTTTGCACTAAATCGGCACCGCCGACCAAACCGCCGATTAACGCCCCCACCAGCATACCGTATg                                                                                                                                                                                 >  1:619568/1‑250 (MQ=255)            gTTAAAA‑TCATCGTCGCT‑AAGGC‑TAATGCCAACAAAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACCGGCGGCTAAAAT‑CCGCATGACCGCCGTGGTAATGCCCTGCGCACCGCCGATCATGAGACTCACGGTTTGCACTAAATCGGCACCGCCGACCAAACCGCCGATTAACGCCCCCACCAGCATACCGTATg                                                                                                                                                                                 >  1:176746/1‑250 (MQ=255)             taaaaa‑TCACCGTCGCA‑AGGGC‑TAATCCCAACAAAGCAC‑GGGCTCCCCCAAAC‑TTATGGGTAAGGGTTC‑CCGCAA‑TCGTG‑TGGGGGCCCCGGAAATCAAC‑CAGCCCGCCCGCAAACACCCGGGCGGCTAAAAC‑CCGCATGCCCGCCGTGGTAATGCCCTGCGCCCCGCCGATCATGAGACTCACGGTTTGCACTAAATCGGCACCGCCGACCAAACCGCCGATTAACGCCCCCACCAGCATACCGTATGcc                                                                                                                                                                               <  1:898287/249‑1 (MQ=255)                                            caacaaAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACCGGCGGCTAAAAT‑CCGCATGACCGCCGTGGTAATGCCCTGCGCACCGCCGATCATGAGACTCACGGTTTGCACTAAATCGGCACCGCCGACCAAACCGCCGATTAACGCCCCCACCAGCATACCGTATGCCGGCGATACT‑‑TTTTTCAAAATCaagag                                                                                                                                                   >  1:539886/1‑249 (MQ=255)                                                         ggCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACCGGCGGCTAAAAT‑CCGCATGACCGCCGTGGTAATGCCCTGCGCACCGCCGATCATGAGACTCACGGTTTGCACTAAATCGGCACCGCCGACCAAACCGCCGATTAACGCCCCCACCAGCATACCGTATGCCGGCGATACT‑‑TTTTTCAAAATCAGGAGGATCGCCACCGCaa                                                                                                                                     >  1:507204/1‑251 (MQ=255)                                                             tCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACCGGCGGCTAAAAT‑CCGCATGACCGCCGTGGTAATGCCCTGCGCACCGCCGATCATGAGACTCACGGTTTGCACTAAATCGGCACCGCCGACCAAACCGCCGATTAACGCCCCCACCAGCATACCGTATGCCGGCGATACT‑‑TTTTTc                                                                                                                                                              >  1:109904/1‑222 (MQ=255)                                                             tCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACCGGCGGCTAAAAT‑CCGCATGACCGCCGTGGTAATGCCCTGCGCACCGCCGATCATGAGACTCACGGTTTGCACTAAATCGGCACCGCCGACCAAACCGCCGATTAACGCCCCCACCAGCATACCGTATGCCGGCGATACT‑‑TTTTTCAAAATCAGGAGGATCGCCACCGCAAGTg                                                                                                                                  >  1:482206/1‑250 (MQ=255)                                                             tCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACCGGCGGCTAAAAT‑CCGCATGACCGCCGTGGTAATGCCCTGCGCACCGCCGATCATGAGACTCACGGTTTGCACTAAATCGGCACCGCCGACCAAACCGCCGATTAACGCCCCCACCAGCATACCGTATGCCGGCGATACT‑‑TTTTTCAAAATCAGGAGGATCGCCACCGCAAGTg                                                                                                                                  >  1:276536/1‑250 (MQ=255)                                                             tCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACCGGCGGCTAAAAT‑CCGCATGACCGCCGTGGTAATGCCCTGCGCACCGCCGATCATGAGACTCACGGTTTGCACTAAATCGGCACCGCCGACCAAACCGCCGATTAACGCCCCCACCAGCATACCGTATGCCGGCGATACT‑‑TTTTTCAAAATCAGGAGGATCGCCACCGCAAGTg                                                                                                                                  >  1:282353/1‑250 (MQ=255)                                                             tCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACCGGCGGCTAAAAT‑CCGCATGACCGCCGTGGTAATGCCCTGCGCACCGCCGATCATGAGACTCACGGTTTGCACTAAATCGGCACCGCCGACCAAACCGCCGATTAACGCCCCCACCAGCATACCGTATGCCGGCGATACT‑‑TTTTTCAAAATCAGGAGGATCGCCACCGCAAGTg                                                                                                                                  >  1:334875/1‑250 (MQ=255)                                                                          ttGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACCGGCGGCTAAAAT‑CCGCATGACCGCCGTGGTAATGCCCTGCGCACCGCCGATCATGAGACTCACGGTTTGCACTAAATCGGCACCGCCGACCAAACCGCCGATTAACGCCCCCACCAGCATACCGTATGCCGGCGTTTCC‑‑TTTTTCAAAAACAGGAGGATCGCCACCGCAAGTGCAATGACCGCTc                                                                                                                      >  1:805640/1‑250 (MQ=255)                                                                             gTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACCGGCGGCTAAAAT‑CCGCATGACCGCCGTGGTAATGCCCTGCGCACCGCCGATCATGAGACTCACGGTTTGCACTAAATCGGCACCGCCGACCAAACCGCCGATTAACGCCCCCACCAGCATACCGTATGCCGGCTTCCCT‑‑TTTTACAAAATCAGTAGGATCGCCACCGCAAGTGCAATGACCGCTCCCaa                                                                                                                  >  1:746923/1‑251 (MQ=255)                                                                                                          ttaaaaaagacaaaaccgccccgccccccAACACCCCGGGGGCTAAAAT‑CCGCATGACCGCCGTGGTAATGCCCTGCGCACCGCCGATCATGAGACTCAGGGTTTGCACTAAATCGGCACCGCCGACCAAACCGCCGATTAACGCCCCCACCAGCATACCGTATGCCGGCGATACT‑‑TTTTTCAAAATCAGGAGGATCGCCACCGCAAGTGCAATGACCGCTCCCAACGCTGAGACTGTCGTCATATAATtc                                                                                         <  1:723055/230‑1 (MQ=255)                                                                                                           gggcgcgaaaacaacgcgaacccccgcaAACACACGGGCGGCAAAACC‑CCGGATTCCCGCCGTGGTAGTGCCCTGCGCCCCGCCGATCATGAGCCCCACGGTTTGCACTAAACCGGCCCCGCCGACCAAACCGCCGATTAACGCCCCCACCAGCATACCGTATGCCGGCGATACT‑‑TTTTTCAAGATCAGGAGGATCGCCACCGCAAGTGCAATGACCGCTCCCAACGCTGAGACTGTCGTCATATAATtc                                                                                         <  1:691478/228‑1 (MQ=255)                                                                                                                   atcaac‑caGCACCCCCGCAAACACACCGGCGGCAAAAAC‑CCGCAGGCCCGCCGGGGTAATGCCCTGCGCCCCGCCGATCATGGGACTCACGGTTTGCACTAAATCGGCACCGCCGCCCAAACCGCCGATTAACGCCCCCACCAGCATACCGTATGCCGGCGATACT‑‑TTTTTCAAAATCAGGAGGATCGCCACCGCAAGTGCAATGACCGCTCCCAACGCTGAGACTGTCGTCATATAATTCTCCTCTGtga                                                                               <  1:283429/251‑1 (MQ=255)                                                                                                                    acaaaccggcgcgcccgccACCACCCGGGCGGTTAAAAC‑CCGCATGACCGCCGTGGTAAGGCCCTGCGCCCCGCCGATCATGAGACTCACGGTTTGCACTAAATCGGCACCGCCGACCAAACCGCCGATTAACGCCCCCACCAGCATACCGTATGCCGGCGATACT‑‑TTTTTCAAAATCAGGAGGATCGCCACCGCAAGTGCAATGACCGCTCCCAACGCTGAGACTGTCGTCATATAATTCTCCTCTGtga                                                                               <  1:528538/240‑1 (MQ=255)                                                                                                                       aaccaccccccccgccAACACCCGGGCGGCAAAAAC‑CCGCAGGCCCGCCGTGGTAATGCCCTGCGCACCGCCGATCATGAGACTCACGGTTTGCACTAAATCGGCACCGCCGACCAAACCGCCGATTAACGCCCCCACCAGCATACCGTATGCCGGCGATACT‑‑TTTTTCAAAATCAGGAGGATCGCCACCGCAAGTGCAATGACCGCTCCCAACGCTGAGACTGTCGTCATATAATTCTCCTCTGtgatga                                                                            <  1:267786/241‑1 (MQ=255)                                                                                                                         ccagcccgccccccaccccccgggcggc‑AAAATCCCGCATGCCCGCGGTGGTAATGCCCGGCGCACCGCCGATCATGAGACTCACGGTTTGCACTAAATCGGCACCGCCGACCAAACCGCCGATTAACGCCCCCACCAGCATACCGTATGCCGGCGATACT‑‑TTTTTCAAAATCAGGAGGATCGCCACCGCAAGTGCAATGACCGCTCCCAACGCTGAGACTGTCGTCATATAATTCTCCTCTGTGATGAAc                                                                          <  1:74394/229‑1 (MQ=255)                                                                                                                         cacgCACGCCCGCCAACCCGCCGGCGGCTAAAAT‑CCGCAGGACCGCCGTGGGAATGCCCTGGCCACCGCCGATCAGGAGACTCCCGGTTTGCACTAAATCGGCACCGCCGCCCAAACCGCCGATTAACGCCCCCACCAGCATACCGTATGCCGGCGATACT‑‑TTTTTCAAAATCAGGAGGATCGCCACCGCAAGTGCAATGACCGCTCCCAACGCTGAGACTGTCGTCATATAATTCTCCTCTGTGATGAAc                                                                          <  1:748757/248‑1 (MQ=255)                                                                                                                          caGCCCGCCGGCCAACACCCCGGGGGCAAAAAC‑CCGCGTGGCCGCCGTGATAGTGCCCTGCGCACCGCGGATCATGAGACTCGCGGTTTGCACAAAATCGGCACCGCCGACCAAACCGCCGATTAACGCCCCCACCAGCATACCGTATGCCGGCGATACT‑‑TTTTTCAAAATCAGGAGGATCGCCACCGCAAGTGCAATGACCGCTCCCAACGCTGAGACTGTCGTCATATAATTCTCCTCTGTGATGAAc                                                                          <  1:463195/250‑1 (MQ=255)                                                                                                                            gCACGCCCGCCAACACACCGGCGGCTAAAAT‑CCGCATGACCGCCGTGGTAATGCCCTGCGCACCGCCGATCATGAGACTCACGGTTTGCACTAAATCGGCACCGCCGACCAAACCGCCGATTAACGCCCCCACCAGCATACCGTATGCCGGCGATACT‑‑TTTTTCAAAATCAGGAGGATCGCCACCGCAAGTGCaa                                                                                                                               >  1:726222/1‑195 (MQ=255)                                                                                                                                ccccacaaccccccgggcggcTAAAAC‑CCTGGTGACCGCCGTGGTAATGCCCTCCCCACCGCCGATCATGAGACTCACGGTTTGCACTAAATCGGCACCGCCGACCAAACCGCCGATTAACGCCCCCACCAGCATACCGTATGCCGGCGATACT‑‑TTTTTCAAAATCAGGAGGATCGCCACCGCAAGTGCAATGACCGCTCCCAACGCTGAGACTGTCGTCATATAATTCTCCTCTGTGATGAACCGTGTaa                                                                   <  1:508431/236‑1 (MQ=255)                                                                                                                                   cgcaaaCACACGGGCGGCAAAAAC‑CCGCATGCCCGCCGTGGTAATCCCCTGCGCACCGCCGATCATGAGACTCACGGTTTGCACTAAATCGGCACCGCCGACCAAACCGCCGATTAACGCCCCCACCAGCATACCGTATGCCGGCGATACT‑‑TTTTTCAAAATCAGGAGGATCGCCACCGCAAGTGCAATGACCGCTCCCAACGCTGAGACTGTCGTCATATAATTCTCCTCTGTGATGAACCGTGTAAATa                                                                <  1:101076/247‑1 (MQ=255)                                                                                                                                   cgcaaaCACACGGGCGACAAACAT‑CCGCAGGCCCGCCGTGGAAATGCCCTGCGCACCGCCGATCATGAGCCTCACGGTTTGCACTAAATCGGCACCGCCGACCAAACCGCCGATTAACGCCCCCACCAGCATACCGTATGCCGGCGATACT‑‑TTTTTCAAAATCAGGAGGATCGCCACCGCAAGTGCAATGACCGCTCCCAACGCTGAGACTGTCGTCATATAATTCTCCTCTGTGATGAACCGTGTAAATa                                                                <  1:149646/247‑1 (MQ=255)                                                                                                                                                   gcTAAAAT‑CCGCATGACCGCCGTGGTAATGCCCTGCGCACCGCCGATCATGAGACTCACGGTTTGCACTAAATCGGCACCGCCGACCAAACCGCCGATTAACGCCCCCACCAGCATACCGTATGCCGGCGATACT‑‑TTTTTCAAAATCAGGAGGATCGCCACCGCAAGTGCAATGACCGCCCCCAACGCTGAGACTGTCGTCATATAATTCTCCTCTGTGATGAAc                                                                          >  1:135410/1‑225 (MQ=255)                                                                                                                                                     caaaaaccccaaTGACCGCCGTGGTAATGCCCTGCCCCCCCCCAATCATGAGCCTCTCGGTTTGCACTAAATCGGCCCCCCCGACCAAACCGCCGATTAACGCCCCCACCAGCATACCGTATGCCGGCGATACT‑‑TTTTTCAAAATCAGGAGGATCGCCACCGCAAGTGCAATGACCGCTCCCAACGCTGAGACTGTCGTCATATAATTCTCCTCTGTGATGAACCGTGTAAATAACACAAAGGAGattatt                                               <  1:123293/240‑1 (MQ=255)                                                                                                                                                          tgggagatggggataagagacaggCCCTGCGCCCCGCCGATCATGAGACTCACGGCTTGCACGAATTCGGAGCCGCCGACCAAACCGCCGAGTAACGCCCCCACCAGCATGCCGTATGCCGGCGATACT‑‑TGTTTCAAAATCAGGAGGATCGCCACCGCAAGTGCAATGTCCGCTCCCAACGCTGAGACTGTCGTCATATAATTCTCCTCTGTGATGAACCGTGTAAATAACACAAAGGAGATTATTCGccc                                          <  1:394926/228‑1 (MQ=255)                                                                                                                                                                    ccgccgTGGTAATGCCCTGCGCACCGCCGATCATGAGACTCACGGTTCGCACTAAATCGGCACCGCCGACCAAACCGCCGATTAACGCCCCCACCAGCATACCGTATGCCGGCGATACT‑‑TTTTTCAAAATCAGGAGGATCGCCACCGCAAGTGCAATGACCGCTCCCAACGCTGAGACTGTCGTCATATAATTCTCCTCTGtgatg                                                                             <  1:125656/206‑1 (MQ=255)                                                                                                                                                                     cgccgTGGTAATGCCCTGCGCACCGCCGATCATGAGACTCACGGTTTGCACTAAATCGGCACCGCCGACCAAACCGCCGATTAACGCCCCCACCAGCATACCGTATGCCGGCGATACT‑‑TTTTTCAAAATCAGGAGGATCGCCACCGCAAGTGCAATGACCGCTCCCAACGCTGAGAc                                                                                                         <  1:44188/177‑1 (MQ=255)                                                                                                                                                                       ccgTGGTAATGCCCTGCGCACCGCCGATCATGAGACTCACGGTTTGCACTAAATCGGCACCGCCGACCAAACCGCCGATTAACGCCCCCACCAGCATACCGTATGCCGGCGATACT‑‑TTTTTCAAAATCAGGAGGATCGCCACCGCAAGTGCAATGACCGCTCCCAACGCTGAGACTGTCGTCATATAATTCTCCTCTGTGATGAACCGTGTAAATa                                                                >  1:558029/1‑216 (MQ=255)                                                                                                                                                                       ccgTGGTAATGCCCTGCCCCCCGCCGATCATGAGACTCACGGTTTGCACTAAATCGGCACCGCCGCCCAAACCGCCGATTAACGCCCCCACCAGCATACCGTATGCCGGCGATACT‑‑TTTTTCAAAATCAGGAGGATCGCCACCGCAAGTGCAATGACCGCTCCCAACGCTGAGACTGTCGTCATATAATTCTCCTCTGtga                                                                               <  1:680452/201‑1 (MQ=255)                                                                                                                                                                          tGGTAATGCCCTGCGCCCCGCCGATCATGAGACTCACGGTTTGCACTAAATCGGCACCGCCGACCAAACCGCCGATTAACGCCCCCACCAGCATACCGTATGCCGGCGATACT‑‑TTTTTCAAAATCAGGAGGATCGCCACCGCAAGTGCAATGACCGCTCCCAACGCTGAGACTGTCGTCatat                                                                                              <  1:122704/183‑1 (MQ=255)                                                                                                                                                                          tGGTAATGCCCTGCGCACCGCCGATCATGAGACTCACGGTTTGCACTAAATCGGCACCGCCGACCAAACCGCCGATTAACGCCCCCACCAGCATACCGTATGCCGGCGATACT‑‑TTTTTCAAAATCAGGAGGATCGCCACCGCAAGTGCAATGACCGCTCCCAACGCTGAGACTGTCGTCATATAATTCTCCTCTGtga                                                                               <  1:77893/198‑1 (MQ=255)                                                                                                                                                                            gTAATGCCCTGCGCACCGCCGATCATGAGACTCACGGTTTGCACTAAATCGGCACCGCCGACCAAACCGCCGATTAACGCCCCCACCAGCATCCCGTATGCCGGC‑‑TTCTGCTTGTTCAAAATCAAGAGGATCACCACCGCAAGTGCAATGACCGCTCCCAACGCTGAGACTGTCGTCATATAATTCTCCTCTGTGATGAACCGTGTAAATAACACAAAGGAGatta                                                 >  1:876707/1‑226 (MQ=255)                                                                                                                                                                            gTAATGCCCTGCGCACCGCCGATCATGAGACTCACGGTTTGCACTAAATCGGCACCGCCGACCAAACCGCCGATTAACGCCCCCACCAGCATACCGTATGCCGGCGATACT‑‑TTTTTCAAAATCAGGAGGATCGCCACCGCAAGTGCAATGACCGCTCCCAAc                                                                                                                 >  1:670824/1‑162 (MQ=255)                                                                                                                                                                            gTAATGCCCTGCCCCCCGCCGATCATGCGACTCACGGTTGGCACTAAATCGGCACCGCCGCCCAAACCGCCGATTAACGCCCCCACCAGCATACCGTATGCCGGCGATACT‑‑TTTTTCAAAATCAGGAGGATCGCCACCGCAAGTGCAATGACCGCTCCCAACGCTGAGACTGTCGTCATATAATTCTCCTCTGTGATGAACCGTGTAAAt                                                                 <  1:14513/210‑1 (MQ=255)                                                                                                                                                                                 gCCCTGCGCACCGCCGATCATGAGACTCACGGTTTGCACTAAATCGGCACCGCCGACCAAACCGCCGATTAACGCCCCCACCAGCATACCTTATGCCGGCGATACT‑‑TTTTTCAAAATCAGGAGGATCGCCACCGCAAGTGCAATGACCGCTCCCAACg                                                                                                                <  1:405546/158‑1 (MQ=255)                                                                                                                                                                                 gCCCTGCGCACCGCCGATCATGAGACTCACGGTTTGCACTAAATCGGCACCGCCGACCAAACCGCCGATTAACGCCCCCACCAGCATACCGTATGCCGGCGATACT‑‑TTTTTCAAAATCAGGAGGATCGCCACCGCAAGTGCAATGACCGCTCCCAACGCTGAGACTGTCGTCATATAATTCTCCTCTGTGATGAAc                                                                          >  1:15927/1‑196 (MQ=255)                                                                                                                                                                                       cgcACCGCCGATCATGAGCCTCACGGTTTGCCCTAAATCGGCACCGCCGCCCAACCCGCCGATTAACGCCCCCACCAGCATACCGTATGCCGGCGATACT‑‑TTTTTCAAAATCAGGAGGATCGCCACCGCAAGTGCAATGACCGCTCCCAACGCTGAGACTGTCGTCATATAATTCTCCTCTGTGATGAACCGTGTAAATAACACAAAGGAGATTATTCGCCCTTAATGCAAAAATTGCTTTATATaaaaa              <  1:131286/250‑1 (MQ=255)                                                                                                                                                                                           ccgccgATCATGAGACTCACGGTTTGCACTAAATCGGCACCGCCGACCAAACCGCCGATTAACGCCCCCACCAGCATACCGTATGCCGGCGATACT‑‑TTTTTCAAAATCAGGAGGATCGCCACCGCAAGTGCAATGACCGCTCCCAACGCTGAGACTGTCGTCATATAATTCTCCTCTGTGATGAAc                                                                          <  1:74629/186‑1 (MQ=255)                                                                                                                                                                                                  tCATGAGACTCACGGTTTGCACTAAATCGGCACCGCCGACCAAACCGCCGATTAACGCCCCCACCAGCATACCGTATGCCGTCGTCAGC‑‑TTGTTCAAAATCAGGAGGATCGCCACCGCAAGTGCAATGACCGCTCCCAACGCTGAGACTGTCGTCATATAATTCTCCTCTGTGATGAACCGTGTAAATAACACAAAGGAGATTATTCGCCCTTAATGCAAAAATTGCTTTATATAAAAATACAAAAATCtt  >  1:336832/1‑251 (MQ=255)                                                                                                                                                                                                  tCATGAGACTCACGGTTTGCACTAAATCGGCACCGCCGACCAAACCGCCGATTAACGCCCCCACCAGCATACCGTATGCCGGCGATACT‑‑TTTTTCAAAATCAGGAGGATCGCCACCGCAAGTGCAATGAc                                                                                                                           >  1:474059/1‑130 (MQ=255)                                                                                                                                                                                                  tCATGAGACTCACGGTTTGCACTAAATCGGCACCGCCGACCAAACCGCCGATTAACGCCCCCACCAGCATACCGTATGCCGGCGATACT‑‑TTTTTCAAAATCAGGAGGATCGCCACCGCAAGTGCAATGACCGCTCCCAACGc                                                                                                               <  1:185577/142‑1 (MQ=255)                                                                                                                                                                                                        gaCTCACGGTTTGCACTAAATCGGCACCGCCGACCAAACCGCCGATTAACGCCCCCACCAGCATACCGTATGCCGGCGATACT‑‑TTTTTCAAAATCAGGAGGATCGCCACCGCAAGTGCAATGACCGCTCCCAACGCTGAGACTGTCGTCATATAATTCTCCTCTGTGTTGAAc                                                                          <  1:800071/173‑1 (MQ=255)                                                                                                                                                                                                        gaCTCACGGTTTGCACTAAATCGGCACCGCCGACCAAACCGCCGATTAACGCCCCCACCAGCATACCGTATGCCGGCGATACT‑‑TTTTTCAAAATCAGGAGGATCGCCACCGCAAGTGCAATGACCGCTCCCAACGCTGAGACTGTCGTCATATAATTCTCCTCTGTGATGAACCGTGTAAATAACACAAAGgaga                                                    >  1:686423/1‑195 (MQ=255)                                                                                                                                                                                                              cGGTTTGCACTAAATCGGCACCGCCGACCAAACCGCCGATTAACGCCCCCACCAGCATCCCGTATGCCGGCTTTTGC‑‑TTTTTCAAAATCAGGAGGATCGCCACCGCAAGTGCAATGACCGCTCCCAACGCTGAGACTGTCGTCATATAATTCTCCTCTGTGATGAACCGTGTAAATAACACAAAGGAGATTATTCGCCCTTAATGCAAAAATTGCTTTATATAAAAATACaaaaa      >  1:448977/1‑235 (MQ=255)                                                                                                                                                                                                                    gCACTAAATCGGCACCGCCGACCAAACCGCCGATTAACGCCCCCACCAGCATACCGTATGcc                                                                                                                                                                               >  1:714218/1‑62 (MQ=255)                                                                                                                                                                                                                    gCACTAAATCGGCACCGCCGACCAAACCGCCGATTAACGCCCCCACCAGCATACCGTATGCCGGCGATACT‑‑TTTTTCAAAATCAGGAGGATCGCCACCGCAAGTGCAATGACCGCTCCCAACGCTGAGACTGTCGTCATATAATTCTCCTCTGTGATGAACCGTGTAAATAACACAAAGGAGATTATTCGCCCTTAATGCAAAAATTGCtt                        <  1:129039/211‑1 (MQ=255)                                                                                                                                                                                                                         aaaTCGGCCCCGCCGACCAAACCGCCGATTAACGCCCCCACCAGCATACCGTATGCCGGCGATACT‑‑TTTTTCAAAATCAGGAGGATCGCCACCGCAAGTGCAATGACCGCTCCCAACGCTGAGACTGTCGTCATATAATTctcc                                                                                      <  1:757509/144‑1 (MQ=255)                                                                                                                                                                                                                          aaccGGCACCGCCGACCAAACCGCCGATTAACGCCCCCACCAGCATACCGTATGCCGGCGATACT‑‑TTTTTCAAAATCAGGAGGATCGCCACCGCAAGTGCAATGACCGCTCCCAACGCTGAGACTGTCGTCATATaa                                                                                            <  1:812750/134‑1 (MQ=255)                                                                                                                                                                                                                          aaTCGGCACCGCCGACCAAACCGCCGATTAACGCCCCCACCAGc                                                                                                                                                                                           >  1:552882/1‑44 (MQ=255)                                                                                                                                                                                                                                                                |                                                                                                                                                                                               CGCCAACAGCTGTTAAAA‑TCATCGTCGCT‑AAGGC‑TAATGCCAACAAAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCAGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACCGGCGGCTAAAAT‑CCGCATGACCGCCGTGGTAATGCCCTGCGCACCGCCGATCATGAGACTCACGGTTTGCACTAAATCGGCACCGCCGACCAAACCGCCGATTAACGCCCCCGCCAGCATACCGTATGCCGGCGATACT‑‑TTTTTCAAAATCAGGAGGATCGCCACCGCAAGTGCAATGACCGCTCCCAACGCTGAGACTGTCGTCATATAATTCTCCTCTGTGATGAACCGTGTAAATAACACAAAGGAGATTATTCGCCCTTAATGCAAAAATTGCTTTATATAAAAATACAAAAATCTT  >  1/2057575‑2058008

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑