BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	2,057,672	A→G	intergenic (‑157929/–)	unknown ← / –	tRNA‑Met‑CAT/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	2,057,672	0	A	G	98.6%	189.8 / ‑4.5	69	intergenic (‑157929/–)	unknown/–	tRNA‑Met‑CAT/–
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (48/20); minor base . (0/1); total (48/21)
Fisher's exact test for biased strand distribution p-value = 3.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

GCATTGGGGTTCGGCGACATAATATTCCCCGCTTTACCACCACCGATCATCGCCAGCAAAATCGCCGGTTTGGATAAAT‑CGGTACGACGCGCCAAGGCAAGGGCAAT‑CGG‑TGATACGGTAATCA‑CCGCCACATCAACGAACACGCCAACAGCTGTTAAAA‑TCATCGTCGCT‑AAGGC‑TAATGCCAACAAAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCAGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACCGGCGGCTAAAATCCGCATGACCGCCGTGGTAATGCCCTGCGCACCGCCGATCATGAGACTCACGGTTTGCACTAAATCGGCACCGCCGACCAAACCGCCGATTAACGCCCCCGCCAGCATACCGTATGCCGGCGATACTTTTTTCAAAATCAGGAGGATCGCCACCGCAAGTGCAATGACCGCTCCCAA  >  1/2057433‑2057896

gCATTGGGGTTCGGCGACATAATATTCCCCGCTTTACCACCACCGATCATCGCCAGCAAAATCGCCGGTTTGGATAAAT‑CGGTACGACGCGCCAAGGCAAGGGCAAT‑CGG‑TGATACGGTAATCA‑CCGCCACATCAACGAACACGCCAACAGCTGTTAAAA‑TCATCGTCGCT‑AAGGC‑TAATGCCAACAAAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑aat                                                                                                                                                                                                                         >  1:27904/1‑250 (MQ=255)

gCATTGGGGTTCGGCGACATAATATTCCCCGCTTTACCACCACCGATCATCGCCAGCAAAATCGCCGGTTTGGATAAAT‑CGGTACGACGCGCCAAGGCAAGGGCAAT‑CGG‑TGATACGGTAATCA‑CCGCCACATCAACGAACACGCCAACAGCTGTTAAAA‑TCATCGTCGCT‑AAGGC‑TAATGCCAACAAAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑aat                                                                                                                                                                                                                         >  1:644311/1‑250 (MQ=255)

gCATTGGGGTTCGGCGACATAATATTCCCCGCTTTACCACCACCGATCATCGCCAGCAAAATCGCCGGTTTGGATAAAT‑CGGTACGACGCGCCAAGGCAAGGGCAAT‑CGG‑TGATACGGTAATCA‑CCGCCACATCAACGAACACGCCAACAGCTGTTAAAA‑TCATCGTCGCT‑AAGGC‑TAATGCCAACAAAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑aat                                                                                                                                                                                                                         >  1:803116/1‑250 (MQ=255)

gCATTGGGGTTCGGCGACATAATATTCCCCGCTTTACCACCACCGATCATCGCCAGCAAAATCGCCGGTTTGGATAAAT‑CGGTACGACGCGCCAAGGCAAGGGCAAT‑CGG‑TGATACGGTAATCA‑CCGCCACATCAACGAACACGCCAACAGCTGTTAAAA‑TCATCGTCGCT‑AAGGC‑TAATGCCAACAAAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑aat                                                                                                                                                                                                                         >  1:486863/1‑250 (MQ=255)

gCATTGGGGTTCGGCGACAAAATATTCCCCGCTTTCCCCCCCCCGATCACCGCCAACAAAATCGCCGTTTTGGATAAAT‑CGGTACGACGCGCCAGGGCAAGGGCAAT‑CGG‑TGATACGTTAATCA‑CCGCCACATCAACGAACACGCCAACAGCTGTTAAAA‑TCATCGTCGCT‑AAGGC‑TAATGCCAACAAAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑aat                                                                                                                                                                                                                         <  1:127505/250‑1 (MQ=255)

     ggggTTCGGCGACATAATATTCCCCGCTTTACCACCACCGATCATCGCCAGCAAAATCGCCGGTTTGGATAAAT‑CGGTACGACGCGCCAAGGAAAGGGCAAT‑CGG‑TGATACGGTAATCA‑CCGCCACATCAACGAACACGCCAACAGCTGTTAAAA‑TCATCGTCGCT‑AAGGC‑TAATGCCAACAAAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑aatcaat‑c                                                                                                                                                                                                                   <  1:406691/250‑1 (MQ=255)

         ttCGGCGACATAATATTCCCCGCTTTCCCACCCCCGATCATCGCCAGCAAAATCGCCGGTTTGGATAAAT‑CGGTACGACGCGCAAAGGCAAGGGCAAT‑CGG‑TGATACGGTAATCA‑CCGCCACATCAACGAACACGCCAACAGCTGTTAAAA‑TCATCGTCGCT‑AAGGC‑TAATGCCAACAAAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCa                                                                                                                                                                                                               <  1:81940/250‑1 (MQ=255)

                aCATAATATTCCCCGCTTTAACACCACCGATCATCGCCAGCAAAATCGCCGGTTTGGATAAAT‑CGGTACGACGCGCCAAGGCAAGGGCAAT‑CGG‑TGATACGGTAATCA‑CCGCCACATCAACGAACACGCCAACAGCTGTTAAAA‑TCATCGTCGCT‑AAGGC‑TAATGCCAACAAAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCAcgcccgc                                                                                                                                                                                                        >  1:836146/1‑250 (MQ=255)

                  ataataTTCCCCGCTTTACCACCACCGATCATCGCCAGCAAAATCGCCGGTTTGGATAAAT‑CGGTACGACGCGCCAAGGCAAGGGCAAT‑CGG‑TGATACGGTAATCA‑CCGCCACATCAACGAACACGCCAACAGCTGTTAAAA‑TCATCGTCGCT‑AAGGC‑TAATGCCAACAAAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCaa                                                                                                                                                                                                     >  1:118225/1‑251 (MQ=255)

                   taataTTCCCCGCTTTACCACCACCGATCATCGCCAGCAAAATCGCCGGTTTGGATAAAT‑CGGTACGACGCGCCAAGGCAAGGGCAAT‑CGG‑TGATACGGTAATCA‑CCGCCACATCAACGAACACGCCAACAGCTGTTAAAA‑TCATCGTCGCT‑AAGGC‑TAATGCCAACAAAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCaa                                                                                                                                                                                                     >  1:764437/1‑250 (MQ=255)

                   aaaattcccccGCTTTCCCCCCCCCGATCATCGCCAGAAAAATCGCCGGTTTGGAAAAAT‑CGGTACGACGCGCCAAGGCAAGGGCAAT‑CGG‑TGATCCGGTAATCA‑CCGCCACATCAACGAACACGCCAACAGCTGTTAAAA‑TCATCGTCGCT‑AAGGC‑TAATGCCAACAAAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAac                                                                                                                                                                                                    <  1:309794/244‑1 (MQ=255)

                               cTTTACCACCACCGATCATCGCCAGCAAAATCGCCGGTTTGGATAAAT‑CGGTACGACGCGCCAAGGCAAGGGCAAT‑CGG‑TGATACGGTAATCA‑CCGCCACATCAACGAACACGCCAACAGCTGTTAAAA‑TCATCGTCGCT‑AAGGC‑TAATGCCAACAAAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACcggcgg                                                                                                                                                                                          >  1:901697/1‑249 (MQ=255)

                                  tccccccaccGATCATCGCCAGCAAAATCGCCGGTTTGGATAAAT‑CGGTACGACGCGCCAAGGAAAGGGCAAT‑CGG‑TGATACGGTAATCA‑CCGCCACATCAACGAACACGCCAACAGCTGTTAAAA‑TCATCGTCGCT‑AAGGC‑TAATGCCAACAAAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCa                                                                                                                                                                                                               <  1:745350/220‑1 (MQ=255)

                                  tACCACCACCGATCATCGCCAGCAAAATCGCCGGTTTGGATAAAT‑CGGTACGACGCGCCAAGGCAAGGGCAAT‑CGG‑TGATACGGTAATCA‑CCGCCACATCAACGAACACGCCAACAGCTGTTAAAA‑TCATCGTCGCT‑AAGGC‑TAATGCCAACAAAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACCGGCGGCTaaa                                                                                                                                                                                     >  1:603451/1‑251 (MQ=255)

                                     cccccccgtccatcccaaaaaaattCGCCGGTTTGGATAAAT‑CGTAACGCCCCGCAAAGGCAGGGGCAAT‑GGG‑TGATACGGAAATCC‑CCGCCACATCAACGAACACGCCAACAGCTGTTAAAA‑TCATCGTCGCT‑AAGGC‑TAATGCCAACAAAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACCGGCGGCTAAAATc                                                                                                                                                                                  <  1:616837/227‑1 (MQ=255)

                                                         aaaacccccGGTTTTGAAAAATCCGGTCCGCCGCCCCAAGG‑AAGGGCAATCCGG‑TGACCCGGAAATCC‑CCGCCACATCAACGAACACGCCAACAGCTGTTAAAA‑TCATCGTCGCT‑AAGGC‑TAATGCCAACAAAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACCGGCGGCTAAAATCCGCATGACCGCCGTGGTaa                                                                                                                                                               <  1:26495/244‑1 (MQ=255)

                                                                   gTTTGGATAAAT‑CGGTACGACGCGCAAAGGCAAGGGCAAT‑CGG‑TGATACGGTAATCA‑CCGCCACATCAACGAACACGCCAACAGCTGTTAAAA‑TCATCGTCGCT‑AAGGC‑TAATGCCAACAAAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACAcc                                                                                                                                                                                               <  1:784030/208‑1 (MQ=255)

                                                                            aaT‑CGGTACGACGCGCCAAGGCAAGGGCAAT‑CGG‑TGATACGGTAATCA‑CCGCCACATCAACGAACACGCCAACAGCTGTTAAAA‑TCATCGTCGCT‑AAGGC‑TAATGCCAACAAAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACCGGCGGCTAAAATCCGCATGACCGCCGTGGTAATGCCCTGCGCACCGCCGAt                                                                                                                                            >  1:847149/1‑250 (MQ=255)

                                                                             aagcggtccgccgcgCCAAGGAAAGGGCAAT‑GGG‑TAATAGGGAAACCC‑CCCCCACAACAACGAACCCCCCAAAAGGTGTTAAAA‑TCATCGTCGCT‑AAGGC‑TAAGGCCAACAAAGCCC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACCGGCGGCTAAAATCCGCATGACCGCCGTGGTAATGCCCTGCGCACCGCCGATc                                                                                                                                           <  1:83128/240‑1 (MQ=255)

                                                                              acccggaccgccgcccAAGGGAAGGGCAAT‑TGG‑TTAATCGGTAAACCCCCGCCACATCAACGAACACGCCAACAGCTGTTAAAA‑TCATCGTCGCT‑AAGGC‑TAATGCCAACAAAGCAC‑GGGCTCCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACCGGCGGCTAAAATCCGCATGACCGCCGTGGTAATGCCCTGCGCACCGCCGATc                                                                                                                                           <  1:12690/237‑1 (MQ=255)

                                                                                  gTACGACGCGCCAAGGCAAGGGCAAT‑CGG‑TGATACGGTAATCA‑CCGCCACATCAACGAACACGCCAACAGCTGTTAAAA‑TCATCGTCGCT‑AAGGC‑TAATGCCAACAAAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACCGGCGGCTAAAATCCGCATGACCGCCGTGGTa                                                                                                                                                                >  1:262721/1‑225 (MQ=255)

                                                                                    ccgccgccccAAGGCAGGGGAATT‑GGG‑TGATCCGGTACCCC‑CCGCCACATCAACGAACACGCCAACAGCTGTTAAAA‑TCATCGTCGCT‑AAGGC‑TAATGCCAACAAAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACCGGCGGCTAAAATCCGCATGACCGCCGTGGTAATGCCCTGCGCACCGCCGATCATGAGAc                                                                                                                                    <  1:767/243‑1 (MQ=255)

                                                                                      cgccgcccAAGGGAAGGGGAAT‑GGGTTGATACGGAAACCC‑CCGCCACATCAACGAACACGCCAACAGCTGTTAAAA‑TCATCGTCGCT‑AAGGC‑TAATGCCAACAAAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACCGGCGGCTAAAATCCGCATGACCGCCGTGGTAATGCCCTGCGCACCGCCGATCATGAGACt                                                                                                                                   <  1:54930/245‑1 (MQ=255)

                                                                                                ggcaaggGCAAT‑CGG‑TGATACGGTAATCA‑CCGCCACATCAACGAACACGCCAACAGCTGTTAAAA‑TCATCGTCGCT‑AAGGC‑TAATGCCAACAAAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACCGGCGGCTAAAATCCGCATGACCGCCGTGGTAATGCCCTGCGCACCGCCGATCATGAGAc                                                                                                                                    >  1:173085/1‑239 (MQ=255)

                                                                                                ggcaaggGCAAT‑CGG‑TGATACGGTAATCA‑CCGCCACATCAACGAACACGCCAACAGCTGTTAAAA‑TCATCGTCGCT‑AAGGC‑TAATGCCAACAAAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACCGGCGGCTAAAATCCGCATGACCGCCGTGGTAATGCCCTGCGCACCGCCGATCATGAGACTCACGGTTTGCa                                                                                                                        >  1:421500/1‑251 (MQ=255)

                                                                                                     gggCAAT‑CGG‑TGATACGGTAATCA‑CCGCCACATCAACGAACACGCCAACAGCTGTTAAAA‑TCATCGTCGCT‑AAGGC‑TAATGCCAACAAAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACCGGCGGCTAAAATCCGCATGACCGCCGTGGTAATGccc                                                                                                                                                          >  1:1144/1‑212 (MQ=255)

                                                                                                      ggCAAT‑CGG‑TGATACGGTAATCA‑CCGCCACATCAACGAACACGCCAACAGCTGTTAAAA‑TCATCGTCGCT‑AAGGC‑TAATGCCAACAAAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑aatcaat‑c                                                                                                                                                                                                                   >  1:832117/1‑154 (MQ=255)

                                                                                                      ggCAAT‑CGG‑TGATACGGTAATCA‑CCGCCACATCAACGAACACGCCAACAGCTGTTAAAA‑TCATCGTCGCT‑AAGGC‑TAATGCCAACAAAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACcgg                                                                                                                                                                                             >  1:234567/1‑176 (MQ=255)

                                                                                                      ggCAAT‑CGG‑TGATACGGTAATCA‑CCGCCACATCAACGAACACGCCAACAGCTGTTAAAA‑TCATCGTCGCT‑AAGGC‑TAATGCCAACAAAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACCGGCGGCTAAAATCCGCATGACCGCCGTGGTAATGCCCTGCGCACCGCCGATCATGAGACTCACGGTTTGCACTAAAt                                                                                                                  >  1:695224/1‑251 (MQ=255)

                                                                                                             cGG‑TGATACGGTAATCA‑CCGCCACATCAACGAACACGCCAACAGCTGTTAAAA‑TCATCGTCGCT‑AAGGC‑TAATGCCAACAAAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCa                                                                                                                                                                                                               >  1:668853/1‑152 (MQ=255)

                                                                                                             atgggtaatcggtaaacccccGCCACATCACCGAACACGCAAACAGCTGTTAAAA‑TCATCGTCGCT‑AAGGC‑TAATGCAAACAAAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAACGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACCGGCGGCTAAAATCCGCATGACCGCCGTGGTAATGCCCTGCGCACCGCCGATCATGAGACTCACGGTTTGCACTAAATCGGc                                                                                                              <  1:787945/232‑1 (MQ=255)

                                                                                                               g‑TGATACGGTAATCA‑CCGCCACATCAACGAACACGCCAACAGCTGTTAAAA‑TCATCGTCGCT‑AAGGC‑TAATGCCAACAAAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACCGGCGGCTAAAATCCGCATGACCGCCGTGGTAATGCCCTGCGCACCGCCGATCATGAGACTCACGGTTTGCACTAAATCGGCAccg                                                                                                          >  1:830781/1‑251 (MQ=255)

                                                                                                               g‑TGATACGGTAATCA‑CCGCCACATCAACGAACACGCCAACAGCTGTTAAAA‑TCATCGTCGCT‑AAGGC‑TAATGCCAACAAAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACCGGCGGCTAAAATCCGCATGACCGCCGTGGTAATGCCCTGCGCACCGCCGATCATGAGACTCACGGTTTGCACTAAATCGGCAccg                                                                                                          >  1:537007/1‑251 (MQ=255)

                                                                                                               g‑TGATACGGTAATCA‑CCGCCACATCAACGAACACGCCAACAGCTGTTAAAA‑TCATCGTCGCT‑AAGGC‑TAATGCCAACAAAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACCGGCGGCTAAAATCCGCATGACCGCCGTGGTAATGCCCTGCGCACCGCCGATCATGAGACTCACGGTTTGCACTAAATCGGCAccg                                                                                                          >  1:208567/1‑251 (MQ=255)

                                                                                                                     aCGGTAATCA‑CCGCCACATCAACGAACACGCCAACAGCTGTTAAAA‑TCATCGTCGCT‑AAGGC‑TAATGCCAACAAAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACCGGCGGCTAAAATCCGCATGACCGCCGTGGTAATGCCCTGCGCACCGCCGATCATGAGACTCACGGTTTGCACTAAATCGGCACCGCCGa                                                                                                      >  1:660403/1‑250 (MQ=255)

                                                                                                                     aCGGTAATCA‑CCGCCACATCAACGAACACGCCAACAGCTGTTAAAA‑TCATCGTCGCT‑AAGGC‑TAATGCCAACAAAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACCGGCGGCTAAAATCCGCATGACCGCCGTGGTAATGCCCTGCGCACCGCCGATCATGAGACTCACGGTTTGCACTAAATCGGCACCGCCGa                                                                                                      >  1:26523/1‑250 (MQ=255)

                                                                                                                      cGGTAATCA‑CCGCCACATCAACGAACACGCCAACAGCTGTTAAAA‑TCATCGTCGCT‑AAGGC‑TAATGCCAACAAAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACCGGCGGCTAAAATCCGCATGACCGCCGTGGTAATGcc                                                                                                                                                           >  1:577387/1‑196 (MQ=255)

                                                                                                                       ggTAATCA‑CCGCCACATCAACGAACACGCCAACAGCTGTTAAAA‑TCATCGTCGCT‑AAGGC‑TAATGCCAACAAAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCa                                                                                                                                                                                                               >  1:168974/1‑143 (MQ=255)

                                                                                                                        gTAATCA‑CCGCCACATCAACGAACACGCCAACAGCTGTTAAAA‑TCATCGTCGCT‑AAGGC‑TAATGCCAACAAAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACCGGCGGCTAAAATCCGCATGACCGCCGTGGTAATGc                                                                                                                                                            >  1:426815/1‑193 (MQ=255)

                                                                                                                        gTAATCA‑CCGCCACATCAACGAACACGCCAACAGCTGTTAAAA‑TCATCGTCGCT‑AAGGC‑TAATGCCAACAAAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACCGGCGGCTAAAATCCGCATGACCGCCGTGGTAATGCCCTGCGCACCGCCGATCATGAGACTCACGGTTTGCACTAAATCGGCACCGCCGACCaa                                                                                                  >  1:393208/1‑251 (MQ=255)

                                                                                                                                      caTCAACGAACACGCCAACAGCTGTTAAAA‑TCATCGTCGCT‑AAGGC‑TAATGCCAACAAAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACCGGCGGCTAAAATCCGCATGACCGCCGTGGTAATGCCCTGCGCACCGCCGATCATGAGACTCACGGTTTGCACTAAATCGGCACCGCCGACCAAACCGCCGATTaa                                                                                      >  1:11406/1‑250 (MQ=255)

                                                                                                                                        tCAACGAACACGCCAACAGCTGTTAAAA‑TCATCGTCGCT‑AAGGC‑TAATGCCAACAAAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACCGGCGGCTAAAATCCGCATGACCGCCGTGGTAATGCCCTGCGCACCGCCGATCATGAGACTCACGGTTTGCACTAAATCGGCACCGCCGACCAAACCGCCGATTAACg                                                                                    >  1:685265/1‑250 (MQ=255)

                                                                                                                                         cAACGAACACGCCAACAGCTGTTAAAA‑TCATCGTCGCT‑AAGGC‑TAATGCCAACAAAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACCGGCGGCTAAAATCCGCATGACCGCCGTGGTAATGc                                                                                                                                                            >  1:594370/1‑177 (MQ=255)

                                                                                                                                                  cGCCAACAGCTGTTAAAA‑TCATCGTCGCT‑AAGGC‑TAATGCCAACAAAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACCGGCGGCTAAAATCCGCATGACCGCCGTGGTAATGCCCTGCGCACCGCCGATCATGAGACTCACGGTTTGCACTAAATCGGCACCGCCGACCAAACCGCCGATTAACGCCCCCACCAg                                                                          >  1:869328/1‑250 (MQ=255)

                                                                                                                                                     ccaactcctgtaaaaaccttcgtcgCA‑AAGGC‑TAATCCCACCAAGGCCC‑GGGCTCCCCCCAAC‑TTATGGGAATTGGTTC‑CCGCAA‑CCTTGTTGGC‑GCCCCCG‑AAACAATCCACCCCGCCCGCCACCACCCCGGCGGCTAAAACCCGCATGCCCGCCGTGGTAATGCCCTGCGCACCGCCGATCATGAGACTCACGGTTTGCACTAAATCGGCACCGCCGACCAAACCGCCGATTAACGCCCCCACCAGCAt                                                                       <  1:91509/232‑1 (MQ=255)

                                                                                                                                                      aaCAGCTTTAAAAA‑TCATCGCCGC‑‑AAGGCATAATGCAAACAAGGC‑G‑GGGGTTCCCCCCACTTTATGGGTAA‑GGTTTCCCGCAA‑TCGTGTGGGCGGCACGGA‑AAACAAT‑CAGCACGCCCGCCAACACCCGGGCGCCTAAAATCCGCATGACCGCCGTGGTAATGCCCTGCGCACCGCCGATCATGAGACTCACGGTTTGCACAAAATCGGCACCGCCGACCAAACCGCCGATTAACGCCCCCACCAGCATAc                                                                     <  1:574478/251‑1 (MQ=255)

                                                                                                                                                       aacggctttAAAACTCATCGTCGAA‑AGGGC‑TAATGCCAACAAAGCAC‑GGGCTCCCCCCACT‑TTATTGGAAATGGTTC‑CCGCAA‑TGGTGTGGGCGGCCCCGA‑AACCAAT‑CAGCCCGCCCGCCACCACACCGGCGGCTAAAATCCGCATGACCGCCGTGGTAATGCCCTGCGCACCGCCGATCATGAGACTCACGGTTTGCACTAAATCGGCACCGCCGACCAAACCGCCGATTAACGCCCCCACCAGCATAc                                                                     <  1:857085/244‑1 (MQ=255)

                                                                                                                                                        caacTGTTAAAA‑TCTTCGTC‑CTGAAGAC‑TAATGCCAAC‑AAGCCCGGGGCTTCCCCCAAG‑TT‑TTAGGAATTGTTT‑TCGAAATTGGTTTTGGGGGCACCGG‑AATAAAA‑CAGCACCCCCGCCAACACACCGGCGGCAAAAACCCGCTTGACCGCCGTGGTAATGCCCTGCGCACCGCCGATCATGAGACTCACGGTTTGCACTAAATCGGCACCGCCGACCAAACCGCCGATTAACGCCCCCACCAGCATACCg                                                                   <  1:230556/248‑1 (MQ=255)

                                                                                                                                                           cTGTTAAAA‑TCATCGTCGCT‑AAGGC‑TAATGCCAACAAAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACCGGCGGCTAAAATCCGCATGACCGCCGTGGTAATGCCCTGCGCACCGCCGATCATGAGACTCACGGTTTGCACTAAATCGGCACCGCCGACCAAACCGCCGATTaa                                                                                      >  1:100793/1‑229 (MQ=255)

                                                                                                                                                             gTTAAAA‑TCATCGTCGCT‑AAGGC‑TAATGCCAACAAAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACCGGCGGCTAAAATCCGCATGACCGCCGTGGTAATGCCCTGCGCACCGCCGATCATGAGACTCACGGTTTGCACTAAATCGGCACCGCCGACCAAACCGCCGATTAACGCCCCCACCAGc                                                                         >  1:34620/1‑240 (MQ=255)

                                                                                                                                                             gTTAAAA‑TCATCGTCGCT‑AAGGC‑TAATGCCAACAAAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACCGGCGGCTAAAATCCGCATGACCGCCGTGGTAATGCCCTGCGCACCGCCGATCATGAGACTCACGGTTTGCACTAAATCGGCACCGCCGACCAAACCGCCGATTAACGCCCCCACCAGCATACCGt                                                                  >  1:113706/1‑247 (MQ=255)

                                                                                                                                                             gTTAAAA‑TCATCGTCGCT‑AAGGC‑TAATGCCAACAAAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACCGGCGGCTAAAATCCGCATGACCGCCGTGGTAATGCCCTGCGCACCGCCGATCATGAGACTCACGGTTTGCACTAAATCGGCACCGCCGACCAAACCGCCGATTAACGCCCCCACCAGCATACCGTATg                                                               >  1:619568/1‑250 (MQ=255)

                                                                                                                                                             gTTAAAA‑TCATCGTCGCT‑AAGGC‑TAATGCCAACAAAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACCGGCGGCTAAAATCCGCATGACCGCCGTGGTAATGCCCTGCGCACCGCCGATCATGAGACTCACGGTTTGCACTAAATCGGCACCGCCGACCAAACCGCCGATTAACGCCCCCACCAGCATACCGTATg                                                               >  1:176746/1‑250 (MQ=255)

                                                                                                                                                              taaaaa‑TCACCGTCGCA‑AGGGC‑TAATCCCAACAAAGCAC‑GGGCTCCCCCAAAC‑TTATGGGTAAGGGTTC‑CCGCAA‑TCGTG‑TGGGGGCCCCGGAAATCAAC‑CAGCCCGCCCGCAAACACCCGGGCGGCTAAAACCCGCATGCCCGCCGTGGTAATGCCCTGCGCCCCGCCGATCATGAGACTCACGGTTTGCACTAAATCGGCACCGCCGACCAAACCGCCGATTAACGCCCCCACCAGCATACCGTATGcc                                                             <  1:898287/249‑1 (MQ=255)

                                                                                                                                                                             gCT‑AAGGC‑TAATGCCAACAAAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACCGGCGGCTAAAATCCGCATGACCGCCGTGGTAATGCCCTGCGCACCGCCGATCATGAGAc                                                                                                                                    >  1:529339/1‑166 (MQ=255)

                                                                                                                                                                                   ggC‑TAATGCCAACAAAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACCGGCGGCTAAAATCCGCATGACCGCCGTGGTAATGCCCTGCGCACCGCCGATCATGAGACTCACgg                                                                                                                              >  1:214370/1‑167 (MQ=255)

                                                                                                                                                                                          tGCCAACAAAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAac                                                                                                                                                                                                    <  1:615312/91‑1 (MQ=255)

                                                                                                                                                                                             caacaaAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACCGGCGGCTAAAATCCGCATGACCGCCGTGGTAATGCCCTGCGCACCGCCGATCATGAGACTCACGGTTTGCACTAAATCGGCACCGCCGACCAAACCGCCGATTAACGCCCCCACCAGCATACCGTATGCCGGCGATACTTTTTTCAAAATCaagag                                   >  1:539886/1‑249 (MQ=255)

                                                                                                                                                                                                          ggCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACCGGCGGCTAAAATCCGCATGACCGCCGTGGTAATGCCCTGCGCACCGCCGATCATGAGACTCACGGTTTGCACTAAATCGGCACCGCCGACCAAACCGCCGATTAACGCCCCCACCAGCATACCGTATGCCGGCGATACTTTTTTCAAAATCAGGAGGATCGCCACCGCaa                     >  1:507204/1‑251 (MQ=255)

                                                                                                                                                                                                              tCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACCGGCGGCTAAAATCCGCATGACCGCCGTGGTAATGCCCTGCGCACCGCCGATCATg                                                                                                                                        <  1:856050/132‑1 (MQ=255)

                                                                                                                                                                                                              tCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACCGGCGGCTAAAATCCGCATGACCGCCGTGGTAATGCCCTGCGCACCGCCGATCATGAGACTCACGGTTTGCACTAAATCGGCACCGCCGACCAAACCGCCGATTAACGCCCCCACCAGCATACCGTATGCCGGCGATACTTTTTTc                                              >  1:109904/1‑222 (MQ=255)

                                                                                                                                                                                                              tCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACCGGCGGCTAAAATCCGCATGACCGCCGTGGTAATGCCCTGCGCACCGCCGATCATGAGACTCACGGTTTGCACTAAATCGGCACCGCCGACCAAACCGCCGATTAACGCCCCCACCAGCATACCGTATGCCGGCGATACTTTTTTCAAAATCAGGAGGATCGCCACCGCAAGTg                  >  1:482206/1‑250 (MQ=255)

                                                                                                                                                                                                              tCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACCGGCGGCTAAAATCCGCATGACCGCCGTGGTAATGCCCTGCGCACCGCCGATCATGAGACTCACGGTTTGCACTAAATCGGCACCGCCGACCAAACCGCCGATTAACGCCCCCACCAGCATACCGTATGCCGGCGATACTTTTTTCAAAATCAGGAGGATCGCCACCGCAAGTg                  >  1:334875/1‑250 (MQ=255)

                                                                                                                                                                                                              tCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACCGGCGGCTAAAATCCGCATGACCGCCGTGGTAATGCCCTGCGCACCGCCGATCATGAGACTCACGGTTTGCACTAAATCGGCACCGCCGACCAAACCGCCGATTAACGCCCCCACCAGCATACCGTATGCCGGCGATACTTTTTTCAAAATCAGGAGGATCGCCACCGCAAGTg                  >  1:282353/1‑250 (MQ=255)

                                                                                                                                                                                                              tCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACCGGCGGCTAAAATCCGCATGACCGCCGTGGTAATGCCCTGCGCACCGCCGATCATGAGACTCACGGTTTGCACTAAATCGGCACCGCCGACCAAACCGCCGATTAACGCCCCCACCAGCATACCGTATGCCGGCGATACTTTTTTCAAAATCAGGAGGATCGCCACCGCAAGTg                  >  1:276536/1‑250 (MQ=255)

                                                                                                                                                                                                                    aaC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACCGGCGGCTAAAATCCGCATGACCGCCGTGGTAATGCCCTGCGCACCGCCGATCATGAGACTCACGGTTTGCACTAAATCGGCACCGCCGACCAAAccgc                                                                                             >  1:852369/1‑169 (MQ=255)

                                                                                                                                                                                                                           ttGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACCGGCGGCTAAAATCCGCATGACCGCCGTGGTAATGCCCTGCGCACCGCCGATCATGAGACTCACGGTTTGCACTAAATCGGCACCGCCGACCAAACCGCCGATTAACGCCCCCACCAGCATACCGTATGCCGGCGTTTCCTTTTTCAAAAACAGGAGGATCGCCACCGCAAGTGCAATGACCGCTc      >  1:805640/1‑250 (MQ=255)

                                                                                                                                                                                                                              gTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACCGGCGGCTAAAATCCGCATGACCGCCGTGGTAATGCCCTGCGCAccgc                                                                                                                                                <  1:116516/109‑1 (MQ=255)

                                                                                                                                                                                                                              gTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACCGGCGGCTAAAATCCGCATGACCGCCGTGGTAATGCCCTGCGCACCGCCGATCATGAGACTCACGGTTTGCACTAAATCGGCACCGCCGACCAAACCGCCGATTAACGCCCCCACCAGCATACCGTATGCCGGCTTCCCTTTTTACAAAATCAGTAGGATCGCCACCGCAAGTGCAATGACCGCTCCCaa  >  1:746923/1‑251 (MQ=255)

GCATTGGGGTTCGGCGACATAATATTCCCCGCTTTACCACCACCGATCATCGCCAGCAAAATCGCCGGTTTGGATAAAT‑CGGTACGACGCGCCAAGGCAAGGGCAAT‑CGG‑TGATACGGTAATCA‑CCGCCACATCAACGAACACGCCAACAGCTGTTAAAA‑TCATCGTCGCT‑AAGGC‑TAATGCCAACAAAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCAGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACCGGCGGCTAAAATCCGCATGACCGCCGTGGTAATGCCCTGCGCACCGCCGATCATGAGACTCACGGTTTGCACTAAATCGGCACCGCCGACCAAACCGCCGATTAACGCCCCCGCCAGCATACCGTATGCCGGCGATACTTTTTTCAAAATCAGGAGGATCGCCACCGCAAGTGCAATGACCGCTCCCAA  >  1/2057433‑2057896

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`