Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	2,049,164	C→T	intergenic (‑149421/–)	unknown ← / –	tRNA‑Met‑CAT/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	2,049,164	0	C	T	100.0%	42.4 / NA	16	intergenic (‑149421/–)	unknown/–	tRNA‑Met‑CAT/–
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (8/8);  total (8/8)

ATTACAAGACAACCCGCAACGCAACAGCCGCGAAGTGAAAAAACTCACCGCGCAACACATTGAAACCTTAAACGACCAAATACAGGCGTTACAAAAAATGGTCACCGAACTGCAACGTTGGCACGACAACTGCCAAGGTAACGACTGCCCGGAATGCTCGATTTTGGAGGGCTTGAGTGAGTGAAAAGTGCGGTGATTTTTCATTGCAATTTTAAGTAGAAATATAATTAAAAAATCTCTCACGCTTATCTTTTAAACGTGGCGGGCGCACGGTGGGA  >  1/2048922‑2049199                                                                                                                                                                                                                                                   |                                    aTTACAAGACAACCCGCAACGCAACAGCCGCGAAGTGAAAAAACTCACCGCGCAACACATTGAAACCTTAAACGACCAAATACAGGCGTTACAAAAAATGGTCACCGAACTGCAACGTTGGCACGACAACTGCCAAGGTAACGACTGCCCGGAATGCTCGATTTTGGAGGGCTTGAGTGAGTGAAAAGTGCGGTGATTTTTCATTGCAATTTTAAGTAGAAATATAATTAAAAAATCTCTCATGCTTAt                               >  1:867525/1‑249 (MQ=255)  ttACAAGACAACCCGCAACGCAACAGCCGCGAAGTGAAAAAACTCACCGCGCAACACATTGAAACCTTAAACGACCAAATACAGGCGTTACAAAAAATGGTCACCGAACTGCAACGTTGGCACGACAACTGCCAAGGTAACGACTGCCCGGAATGCTCGATTTTGGAGGGCTTGAGTGAGTGAAAAGTGCGGTGATTTTTCATTGCAATTTTAAGTAGAAATATAATTAAAAAATCTCTCATGCTTATCtt                            >  1:318394/1‑251 (MQ=255)  ttACAAGACAACCCGCAACGCAACAGCCGCGAAGTGAAAAAACTCACCGCGCAACACATTGAAACCTTAAACGACCAAATACAGGCGTTACAAAAAATGGTCACCGAACTGCAACGTTGGCACGACAACTGCCAAGGTAACGACTGCCCGGAATGCTCGATTTTGGAGGGCTTGAGTGAGTGAAAAGTGCGGTGATTTTTCATTGCAATTTTAAGTAGAAATATAATTAAAAAATCTCTCATGCTTATCtt                            >  1:876366/1‑251 (MQ=255)         aCAACCCGCAACGCAACAGCCGCGAAGTGAAAAAACTCACCGCGCAACACATTGAAACCTTAAACGACCAAATACAGGCGTTACAAAAAATGGTCACCGAACTGCAACGTTGGCACGACAACTGCCAAGGTAACGACTGCCCGGAATGCTCGATTTTGGAGGGCTTGAGTGAGTGAAAAGTGCGGTGATTTTTCATTGCAATTTTAAGTAGAAATATAATTAAAAAATCTCTCATGCTTATCTTTTAAAca                     <  1:318061/251‑2 (MQ=255)         aCAACCCGCAACGCAACAGCCGCGAAGTGAAAAAACTCACCGCGCAACACATTGAAACCTTAAACGACCAAATACAGGCGTTACAAAAAATGGTCACCGAACTGCAACGTTGGCACGACAACTGCCAAGGTAACGACTGCCCGGAATGCTCGATTTTGGAGGGCTTGAGTGAGTGAAAAGTGCGGTGATTTTTCATTGCAATTTTAAGTAGAAATATAATTAAAAAATCTCTCATGCTTATCTTTTAAAc                      <  1:312530/250‑1 (MQ=255)         aCAACCCGCAACGCAACAGCCGCGAAGTGAAAAAACTCACCGCGCAACACATTGAAACCTTAAACGACCAAATACAGGCGTTACAAAAAATGGTCACCGAACTGCAACGTTGGCACGACAACTGCCAAGGTAACGACTGCCCGGAATGCTCGATTTTGGAGGGCTTGAGTGAGTGAAAAGTGCGGTGATTTTTCATTGCAATTTTAAGTAGAAATATAATTAAAAAATCTCTCATGCTTATCTTTTAAAc                      <  1:391561/250‑1 (MQ=255)              ccGCAACGCAACAGCCGCGAAGTGAAAAAACTCACCGCGCAACACATTGAAACCTTAAACGACCAAATACAGGCGTTACAAAAAATGGTCACCGAACTGCAACGTTGGCACGACAACTGCCAAGGTAACGACTGCCCGGAATGCTCGATTTTGGAGGGCTTGAGTGAGTGAAAAGTGCGGTGATTTTTCATTGCAATTTTAAGTAGAAATATAATTAAAAAATCTCTCATGCTTATCTTTTAAAcatgcac                <  1:271480/251‑7 (MQ=255)              ccGCAACGCAACAGCCGCGAAGTGAAAAAACTCACCGCGCAACACATTGAAACCTTAAACGACCAAATACAGGCGTTACAAAAAATGGTCACCGAACTGCAACGTTGGCACGACAACTGCCAAGGTAACGACTGCCCGGAATGCTCGATTTTGGAGGGCTTGAGTGAGTGAAAAGTGCGGTGATTTTTCATTGCAATTTTAAGTAGAAATATAATTAAAAAATCTCTCATGCTTATCTTTTAAAcatgcac                <  1:642345/251‑7 (MQ=255)              ccGCAACGCAACAGCCGCGAAGTGAAAAAACTCACCGCGCAACACATTGAAACCTTAAACGACCAAATACAGGCGTTACAAAAAATGGTCACCGAACTGCAACGTTGGCACGACAACTGCCAAGGTAACGACTGCCCGGAATGCTCGATTTTGGAGGGCTTGAGTGAGTGAAAAGTGCGGTGATTTTTCATTGCAATTTTAAGTAGAAATATAATTAAAAAATCTCTCATGCTTATCTTTTAAAcatgcac                <  1:830057/251‑7 (MQ=255)                    cgcaacAGCCGCGAAGTGAAAAAACTCACCGCGCAACACATTGAAACCTTAAACGACCAAATACAGGCGTTACAAAAAATGGTCACCGAACTGCAACGTTGGCACGACAACTGCCAAGGTAACGACTGCCCGGAATGCTCGATTTTGGAGGGCTTGAGTGAGTGAAAAGTGCGGTGATTTTTCATTGCAATTTTAAGTAGAAATATAATTAAAAAATCTCTCATGCTTATCTTTTAAAc                      >  1:727702/1‑239 (MQ=255)                            ccGCGAAGTGAAAAAACTCACCGCGCAACACATTGAAACCTTAAACGACCAAATACAGGCGTTACAAAAAATGGTCACCGAACTGCAACGTTGGCACGACAACTGCCAAGGTAACGACTGCCCGGAATGCTCGATTTTGGAGGGCTTGAGTGAGTGAAAAGTGCGGTGATTTTTCATTGCAATTTTAAGTAGAAATATAATTAAAAAATCTCTCATGCTTATCTTTTAAAcatgcacaattcctataaaat  <  1:702887/251‑21 (MQ=255)                            ccGCGAAGTGAAAAAACTCACCGCGCAACACATTGAAACCTTAAACGACCAAATACAGGCGTTACAAAAAATGGTCACCGAACTGCAACGTTGGCACGACAACTGCCAAGGTAACGACTGCCCGGAATGCTCGATTTTGGAGGGCTTGAGTGAGTGAAAAGTGCGGTGATTTTTCATTGCAATTTTAAGTAGAAATATAATTAAAAAATCTCTCATGCTTATCTTTTAAAcatgcacaattcctataaaa   <  1:788178/250‑20 (MQ=255)                                caaaaaaccggtttcagcgcgCAACACATTGAAACCTTAAACGACCAAATACAGGCGTTACAAAAAATGGTCACCGAACTGCAACGTTGGCACGACAACTGCCAAGGTAACGACTGCCCGGAATGCTCGATTTTGGAGGGCTTGAGTGAGTGAAAAGTGCGGTGATTTTTCATTGCAATTTTAAGTAGAAATATAATTAAAAAATCTCTCATGCTTATCtt                            >  1:195443/18‑221 (MQ=255)                                   gTGAAAAAACTCACCGCGCAACACATTGAAACCTTAAACGACCAAATACAGGCGTTACAAAAAATGGTCACCGAACTGCAACGTTGGCACGACAACTGCCAAGGTAACGACTGCCCGGAATGCTCGATTTTGGAGGGCTTGAGTGAGTGAAAAGTGCGGTGATTTTTCATTGCAATTTTAAGTAGAAATATAATTAAAAAATCTCTCATGCTTATCTTTTAAAcatgcac                >  1:566808/1‑224 (MQ=255)                                                ccGCGCAACACATTGAAACCTTAAACGACCAAATACAGGCGTTACAAAAAATGGTCACCGAACTGCAACGTTGGCACGACAACTGCCAAGGTAACGACTGCCCGGAATGCTCGATTTTGGAGGGCTTGAGTGAGTGAAAAGTGCGGTGATTTTTCATTGCAATTTTAAGTAGAAATATAATTAAAAAATCTCTCATGCTTATCTTTTAAAcatgcac                >  1:353906/1‑211 (MQ=255)                                                                                      gCGTTACAAAAAATGGTCACCGAACTGCAACGTTGGCACGACAACTGCCAAGGTAACGACTGCCCGGAATGCTCGATTTTGGAGGGCTTGAGTGAGTGAAAAGTGCGGTGATTTTTCATTGCAATTTTAAGTAGAAATATAATTAAAAAATCTCTCATGCTTATCTTTTAAAcatgcacaattc           >  1:566515/1‑173 (MQ=255)                                                                                                                                                                                                                                                   |                                    ATTACAAGACAACCCGCAACGCAACAGCCGCGAAGTGAAAAAACTCACCGCGCAACACATTGAAACCTTAAACGACCAAATACAGGCGTTACAAAAAATGGTCACCGAACTGCAACGTTGGCACGACAACTGCCAAGGTAACGACTGCCCGGAATGCTCGATTTTGGAGGGCTTGAGTGAGTGAAAAGTGCGGTGATTTTTCATTGCAATTTTAAGTAGAAATATAATTAAAAAATCTCTCACGCTTATCTTTTAAACGTGGCGGGCGCACGGTGGGA  >  1/2048922‑2049199

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑