Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,996,171	G→A	intergenic (‑96428/–)	unknown ← / –	tRNA‑Met‑CAT/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,996,171	0	G	A	100.0%	157.9 / NA	55	intergenic (‑96428/–)	unknown/–	tRNA‑Met‑CAT/–
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (25/30);  total (25/30)

CGGTTTGCCCTGTTTAAACAGCGTTTCATCATAGGTTTTATCCGATAATAAATCCTGCAACGTCTTCGGCGGAACCGGCTTACCCACCAACGCCGATTCCAATGCTTTCGGATCATCACCCAGTGCATTGCGTTGTAATTGCACCAGAAAGGCGGTAACCAAAACGAAGAAAATAATTAAAGGTAAATATAATTTCTTTTTCATTATCAATACTCGATGATTCTATAAGGGGTTACTGCACGGTTTCTTTACCTAAAATTTTACTAAACCGATAACGGCGGTCGAACAGGCATAATATCCCGCCGAGCACCATAAACAACCCACCAAG‑CCATATCCAGCGGATAAAGGGTTTGTAATACAAGCGCAACGCCCAGGAATTGTCGTCTAATTTTTCACCTAACGCCACATACAAGTCACGGCTGAAACCCCAATCGATCGCGGCTTCGGTCATCGGCATTTTGCTCACGGTGTATAAGCGTTTTT  >  1/1995930‑1996412                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                   cGGTTTGCCCTGTTTAAACAGCGTTTCATCATAGGTTTTATCCGATAATAAATCCTGCAACGTCTTCGGCGGAACCGGCTTACCCACCAACGCCGATTCCAATGCTTTCGGATCATCACCCAGTGCATTGCGTTGTAATTGCACCAGAAAGGCGGTAACCAAAACGAAGAAAATAATTAAAGGTAAATATAATTTCTTTTTCATTATCAATACTCGATGATTCTATAAGGGGTTACTGCACAGTTTCTTTa                                                                                                                                                                                                                                           <  1:314013/251‑1 (MQ=255)         ccTGTTTAAACAGCGTTTCATCATAGGTTTTATCCGATAATAAATCCTGCAACGTCTTCGGCGGAACCGGCTTACCCACCAACGCCGATTCCAATGCTTTCGGATCATCACCCAGTGCATTGCGTTGTAATTGCACCAGAAAGGCGGTAACCAAAACGAAGAAAATAATTAAAGGTAAATATAATTTCTTTTTCATTATCAATACTCGATGATTCTATAAGGGGTTACTGCACAGTTTCTTTACCTAAAAt                                                                                                                                                                                                                                   <  1:876478/251‑1 (MQ=255)            gTTTAAACAGCGTTTCATCATAGGTTTTATCCGATAATAAATCCTGCAACGTCTTCGGCGGAACCGGCTTACCCACCAACGCCGATTCCAATGCTTTCGGATCATCACCCAGTGCATTGCGTTGTAATTGCACCAGAAAGGCGGTAACCAAAACGAAGAAAATAATTAAAGGTAAATATAATTTCTTTTTCATTATCAATACTCGATGATTCTATAAGGGGTTACTGCACAGTTTCTTTACCTAAAAtttt                                                                                                                                                                                                                                >  1:650213/1‑251 (MQ=255)                        tttCATCATAGGTTTTATCCGATAATAAATCCTGCAACGTCTTCGGCGGAACCGGCTTACCCACCAACGCCGATTCCAATGCTTTCGGATCATCACCCAGTGCATTGCGTTGTAATTGCACCAGAAAGGCGGTAACCAAAACGAAGAAAATAATTAAAGGTAAATATAATTTCTTTTTCATTATCAATACTCGATGATTCTATAAGGGGTTACTGCACAGTTTCTTTACCTAAAATTTTACTAAACCGATa                                                                                                                                                                                                                    <  1:660687/251‑1 (MQ=255)                             tcatAGGTTTTATCCGATAATAAATCCTGCAACGTCTTCGGCGGAACCGGCTTACCCACCAACGCCGATTCCAATGCTTTCGGATCATCACCCAGTGCATTGCGTTGTAATTGCACCAGAAAGGCGGTAACCAAAACGAAGAAAATAATTAAAGGTAAATATAATTTCTTTTTCATTATCAATACTCGATGATTCTATAAGGGGTTACTGCACAGTTTCTTTACCTAAAATTTTACTAAACCGATAAcgg                                                                                                                                                                                                                >  1:241191/1‑250 (MQ=255)                                  ggTTTTATCCGATAATAAATCCTGCAACGTCTTCGGCGGAACCGGCTTACCCACCAACGCCGATTCCAATGCTTTCGGATCATCACCCAGTGCATTGCGTTGTAATTGCACCAGAAAGGCGGTAACCAAAACGAAGAAAATAATTAAAGGTAAATATAATTTCTTTTTCATTATCAATACTCGATGATTCTATAAGGGGTTACTGCACAGTTTCTTTACCTAAAATTTTACTAAACCGATa                                                                                                                                                                                                                    <  1:753511/241‑1 (MQ=255)                                     tttATCCGATAATAAATCCTGCAACGTCTTCGGCGGAACCGGCTTACCCACCAACGCCGATTCCAATGCTTTCGGATCATCACCCAGTGCATTGCGTTGTAATTGCACCAGAAAGGCGGTAACCAAAACGAAGAAAATAATTAAAGGTAAATATAATTTCTTTTTCATTATCAATACTCGATGATTCTATAAGGGGTTACTGCACAGTTTCTTTACCTAAAATTTTACTAAACCGATAACGGCGGTCGAAc                                                                                                                                                                                                       <  1:42484/251‑1 (MQ=255)                                               aataaATCCTGCAACGTCTTCGGCGGAACCGGCTTACCCACCAACGCCGATTCCAATGCTTTCGGATCATCACCCAGTGCATTGCGTTGTAATTGCACCAGAAAGGCGGTAACCAAAACGAAGAAAATAATTAAAGGTAAATATAATTTCTTTTTCATTATCAATACTCGATGATTCTATAAGGGGTTACTGCACAGTTTCTTTACCTAAAACTTTACTAAACCGATAACGGCGGTCGAACAGGCataata                                                                                                                                                                                             <  1:173405/251‑1 (MQ=255)                                                     tCCTGCAACGTCTTCGGCGGAACCGGCTTACCCACCAACGCCGATTCCAATGCTTTCGGATCATCACCCAGTGCATTGCGTTGTAATTGCACCAGAAAGGCGGTAACCAAAACGAAGAAAATAATTAAAGGTAAATATAATTTCTTTTTCATTATCAATACTCGATGATTCTATAAGGGGTTACTGCACAGTTTCTTTACCTAAAATTTTACTAAACCGATAACGGCGGTCGAACAGGCATAATATCccgc                                                                                                                                                                                       <  1:160043/251‑1 (MQ=255)                                                                   cggcggAACCGGCTTACCCACCAACGCCGATTCCAATGCTTTCGGATCATCACCCAGTGCATTGCGTTGTAATTGCACCAGAAAGGCGGTAACCAAAACGAAGAAAATAATTAAAGGTAAATATAATTTCTTTTTCATTATCAATACTCGATGATTCTATAAGGGGTTACTGCACAGTTTCTTTACCTAAAATTTTACTAAACCGATAACGGCGGTCGAACAGGCATAATATCCCGCCGAGCACCATAaac                                                                                                                                                                         <  1:50458/251‑1 (MQ=255)                                                                     gcggAACCGGCTTACCCACCAACGCCGATTCCAATGCTTTCGGATCATCACCCAGTGCATTGCGTTGTAATTGCACCAGAAAGGCGGTAACCAAAACGAAGAAAATAATTAAAGGTAAATATAATTTCTTTTTCATTATCAATACTCGATGATTCTATAAGGGGTTACTGCACAGTTTCTTTACCTAAAATTTTACTAAACCGATAACGGCGGTCGAACAGGCATAATATCCCGCCGAGCACCATAaaca                                                                                                                                                                        <  1:372427/250‑1 (MQ=255)                                                                      cggAACCGGCTTACCCACCAACGCCGATTCCAATGCTTTCGGATCATCACCCAGTGCATTGCGTTGTAATTGCACCAGAAAGGCGGTAACCAAAACGAAGAAAATAATTAAAGGTAAATATAATTTCTTTTTCATTATCAATACTCGATGATTCTATAAGGGGTTACTGCACAGTTTCTTTACCTAAAATTTTACTAAACCGATAACGGCGGTCGAACAGGCATAATATCCCGCCGAGCACCATAaacaac                                                                                                                                                                      <  1:719331/251‑1 (MQ=255)                                                                                      accaACGCCGATTCCAATGCTTTCGGATCATCACCCAGTGCATTGCGTTGTAATTGCACCAGAAAGGCGGTAACCAAAACGAAGAAAATAATTAAAGGTAAATATAATTTCTTTTTCATTATCAATACTCGATGATTCTATAAGGGGTTACTGCACAGTTTCTTTACCTAAAATTTTACTAAACCGATAACGGCGGTCGAACAGGCATAATATCCCGCCGAGCACCATAAACAACCCACCAAG‑CCATATcc                                                                                                                                                     <  1:768738/251‑1 (MQ=255)                                                                                                               acgacaattcctgggcgttGCGTTGTAATTGCACCAGAAAGGCGGTAACCAAAACGAAGAAAATAATTAAAGGTAAATATAATTTCTTTTTCATTATCAATACTCGATGATTCTATAAGGGGTTACTGCACAGTTTCTTTACCTAAAATTTTACTAAACCGATAACGGCGGTCGAACAGGCATAATATCCCGCCGAGCACCATAAACAACCCACCAAG‑CCATATCCAGCGGATAAAGGGTTTGTAATAc                                                                                                                              <  1:291991/232‑1 (MQ=255)                                                                                                                 tcatcaCCCAGTGCATTGCGTTGTAATTGCACCAGAAAGGCGGTAACCAAAACGAAGAAAATAATTAAAGGTAAATATAATTTCTTTTTCATTATCAATACTCGATGATTCTATAAGGGGTTACTGCACAGTTTCTTTACCTAAAATTTTACTAAACCGATAACGGCGGTCGAACAGGCATAATATCCCGCCGAGCACCATAAACAACCCACCAAG‑cc                                                                                                                                                           <  1:14025/218‑1 (MQ=255)                                                                                                                 tcatcaCCCAGTGCATTGCGTTGTAATTGCACCAGAAAGGCGGTAACCAAAACGAAGAAAATAATTAAAGGTAAATATAATTTCTTTTTCATTATCAATACTCGATGATTCTATAAGGGGTTACTGCACAGTTTCTTTACCTAAAATTTTACTAAACCGATAACGGCGGTCGAACAGGCATAATATCCCCCCCAGCACCATAAAAAACCCACCAAA‑CCATATCCAGCGGGTAAAGGGGTTGTAATACaaa                                                                                                                           >  1:323490/1‑249 (MQ=255)                                                                                                                           gTGCATTGCGTTGTAATTGCACCAGAAAGGCGGTAACCAAAACGAAGAAAATAATTAAAGGTAAATATAATTTCTTTTTCATTATCAATACTCGATGATTCTATAAGGGGTTACTGCACAGTTTCTTTACCTAAAATTTTACTAAACCGATAACGGCGGTCGAACAGGCATAATATCCCCCCCAGCACCCTaaaa                                                                                                                                                                         >  1:264499/1‑194 (MQ=255)                                                                                                                               aTTGCGTTGTAATTGCACCAGAAAGGCGGTAACCAAAACGAAGAAAATAATTAAAGGTAAATATAATTTCTTTTTCATTATCAATACTCGATGATTCTATAAGGGGTTACTGCACAGTTTCTTTACCTAAAATTTTACTAAACCGATAACGGCGGTCGAACAGGCATAATATCCCGCCGAGCACCATAAACAACCCACCAAG‑CCATATCCAGCGGATAAAGGGTTTGTAATACAAGCGCAACGCCCAGGaa                                                                                                            <  1:849201/251‑1 (MQ=255)                                                                                                                                  gCGTTGTAATTGCACCAGAAAGGCGGTAACCAAAACGAAGAAAATAATTAAAGGTAAATATAATTTCTTTTTCATTATCAATACTCGATGATTCTATAAGGGGTTACTGCACAGTTTCTTTACCTAAAATTTTACTAAACCGATAACGGCGGTCGAACAGGCATAATATCCCGCCGAGCACCATAaac                                                                                                                                                                         <  1:835650/188‑1 (MQ=255)                                                                                                                                  gCGTTGTAATTGCACCAGAAAGGCGGTAACCAAAACGAAGAAAATAATTAAAGGTAAATATAATTTCTTTTTCATTATCAATACTCGATGATTCTATAAGGGGTTACTGCACAGTTTCTTTACCTAAAATTTTACTAAACCGATAACGGCGGTCGAACAGGCATAATATCCCGCCGAGCACCATAAACAACCCACCAAG‑CCATATCCAGCGGATAAAGGGTTTGTAATAc                                                                                                                              <  1:882070/230‑1 (MQ=255)                                                                                                                                  gCGTTGTAATTGCACCAGAAAGGCGGTAACCAAAACGAAGAAAATAATTAAAGGTAAATATAATTTCTTTTTCATTATCAATACTCGATGATTCTATAAGGGGTTACTGCACAGTTTCTTTACCTAAAATTTTACTAAACCGATAACGGCGGTCGAACAAGCATAATATCCCGCCGAGCACCATAAAAAAACCACCCAG‑CCATATCCAGCGGGTAAAGGGGTTGTAAAAAAAACGCAAAGCCCAGGaaatg                                                                                                         >  1:1581/1‑248 (MQ=255)                                                                                                                                       gTAATTGCACCAGAAAGGCGGTAACCAAAACGAAGAAAATAATTAAAGGTAAATATAATTTCTTTTTCATTATCAATACTCGATGATTCTATAAGGGGTTACTGCACAGTTTCTTTACCTAAAATTTTACTAAACCGATAACGGCGGTCGAACAGGCATAATATCCCGCCGAGCACCATAAACAACCCACCaa                                                                                                                                                               <  1:309463/193‑1 (MQ=255)                                                                                                                                              cACCAGAAAGGCGGTAACCAAAACGAAGAAAATAATTAAAGGTAAATATAATTTCTTTTTCATTATCAATACTCGATGATTCTATAAGGGGTTACTGCACAGTTTCTTTACCTAAAATTTTACTAAACCGATAACGGCGGTCGAACAGGCATAATATCCCGCCCAGCACCATAAACAACCCACCAAA‑CCAAATCCCGCGGAAAAAAGGGTTGTAATAAAAACGCAACCCCCCGGaacttgctcttataaa                                                                                              >  1:616174/1‑236 (MQ=255)                                                                                                                                                        gCGGTAACCAAAACGAAGAAAATAATTAAAGGTAAATATAATTTCTTTTTCATTATCAATACTCGATGATTCTATAAGGGGTTACTGCACAGTTTCTTTACCTAAAATTTTACTAAACCGATAACGGCGGTCGAACAGGCATAATATCCCGCCGAGCACCATAAACAAACCACCAAA‑CCCTATcc                                                                                                                                                     >  1:490189/1‑185 (MQ=255)                                                                                                                                                           gTAACCAAAACGAAGAAAATAATTAAAGGTAAATATAATTTCTTTTTCATTATCAATACTCGATGATTCTATAAGGGGTTACTGCACAGTTTCTTTACCTAAAATTTTACTAAACCGATAACGGCGGTCGAACAGGCataata                                                                                                                                                                                             <  1:10224/143‑1 (MQ=255)                                                                                                                                                             aaCCAAAACGAAGAAAATAATTAAAGGTAAATATAATTTCTTTTTCATTATCAATACTCGATGATTCTATAAGGGGTTACTGCACAGTTTCTTTACCTAAAATTTTACTAAACCGATAACGGCGGTCGAAAAGGCAAAATATCCCGCCCAGCACCATAAACAACCCACCAAG‑CCATATCCCGCGGGTAAAGGGGTTGTAATAAAAACGCAACGCCCCGGAATTGTCGTCTAATTTTTCACCTAACCCcaca                                                                              >  1:328250/1‑251 (MQ=255)                                                                                                                                                                    aCGAAGAAAATAATTAAAGGTAAATATAATTTCTTTTTCATTATCAATACTCGATGATTCTATAAGGGGTTACTGCACAGTTTCTTTACCTAAAATTTTACTAAACCGATAACGGCGGTCGAACAGGCATAATATCCCGCCGAGCACCATAAACAACCCACCAAG‑CCATATCCAGCGGATAAAGGGTTTGTAATACAAGCGCAACGCCCAGGAATTGTCGTCTAATTTTTCACCTAACGCCACATACAAGt                                                                       <  1:738503/251‑1 (MQ=255)                                                                                                                                                                               aatTAAAGGTAAATATAATTTCTTTTTCATTATCAATACTCGATGATTCTATAAGGGGTTACTGCACAGTTTCTTTACCTAAAATTTTACTAAACCGATAACGGCGGTCGAACAGGCATAATATCCCCCCCAGCACCATAAAAAACCCACCAAA‑CCCTATCCAGCGGATAAAAGGGTTGTAATACAAGCGCAACGCCCAGGAATTGTCGTCTAATTTTTCACCTAACGCCACATACAAATCACGGCTGaaa                                                            >  1:669357/1‑251 (MQ=255)                                                                                                                                                                                           atatAATTTCTTTTTCATTATCAATACTCGATGATTCTATAAGGGGTTACTGCACAGTTTCTTTACCTAAAATTTTACTAAACCGATAACGGCGGTCGAACAGGCATAATATCCCGCCGAGCACCATAaacaaacc                                                                                                                                                                    >  1:86997/1‑133 (MQ=255)                                                                                                                                                                                           atatAATTTCTTTTTCATTATCAATACTCGATGATTCTATAAGGGGTTACTGCACAGTTTCTTTACCTAAAATTTTACTAAACCGATAACGGCGGTCGAACAGGCATAATATCCCCCCGAGCACCATAAACAACCCACCAAG‑CCATATCCAGCGGATAAAGGGTTTGTAATACAAACGCAACGCCCCGGAAATgt                                                                                                        >  1:295439/1‑195 (MQ=255)                                                                                                                                                                                            tataATTTCTTTTTCATTATCAATACTCGATGATTCTATAAGGGGTTACTGCACAGTTTCTTTACCTAAAATTTTACTAAACCGATAACGGCGGTCGAACAGGCATAATATCCCGCCGAGCACCATAaac                                                                                                                                                                         >  1:110159/1‑130 (MQ=255)                                                                                                                                                                                                   tCTTTTTCATTATCAATACTCGATGATTCTATAAGGGGTTACTGCACAGTTTCTTTAc                                                                                                                                                                                                                                          <  1:326094/58‑1 (MQ=255)                                                                                                                                                                                                             tATCAATACTCGATGATTCTATAAGGGGTTACTGCACAGTTTCTTTACCTAAAATTTTACTAAACCGATAACGGCGGTCGAACAGGCataata                                                                                                                                                                                             >  1:221270/1‑93 (MQ=255)                                                                                                                                                                                                              aTCAATACTCGATGATTCTATAAGGGGTTACTGCACAGTTTCTTTACCTAAAATTTTACTAAACCGATAACGGCGGTCGAACAGGCATAATATCCCGCCGAGCACCATAAACAACCCACCAAG‑CCATATCCAGCGGATAAAGGGTTTGTAATACAAGCGCAACGCCCAGGAATTGTCGTCTAATTTTTCACCTAACGCCACATACAAGTCACGGCTGAAAccc                                                         <  1:185144/223‑1 (MQ=255)                                                                                                                                                                                                               tCAATACTCGATGATTCTATAAGGGGTTACTGCACAGTTTCTTTACCTAAAATTTTACTAAACCGATAACGGCGGTCGAACAGGCATAATATCCCGCCGAGCCCCCTAAACAACCCACCAAG‑CCATATCCAGCGGAAAAAGGGTTTGTAATACAAGCGCAACGCCCAGGGATTGTTGTCTAATTTTTCACCTAACGCCACATACAAGACACCGCCGAAACCCCAATCGATCGCGGCTTCCGTCATCGGCAt                            >  1:754697/1‑251 (MQ=255)                                                                                                                                                                                                                  aTACTCGATGATTCTATAAGGGGTTACTGCACAGTTTCTTTACCTAAAATTTTACTAAACCGATAACGGCGGTCGAACAGGCATAATATCCCGCCGAGCACCATAaacaac                                                                                                                                                                      <  1:510803/111‑1 (MQ=255)                                                                                                                                                                                                                  aTACTCGATGATTCTATAAGGGGTTACTGCACAGTTTCTTTACCTAAAATTTTACTAAACCGATAACGGCGGTCGAACAGGCATAATATCCCCCCGAGCACCATAAACAACCCACCAAG‑CCATATCCAGCGGGTAAAGGGGTTGTAATACAAGCGCAACGCCCAGGAATTGTCGTCTAATTTTTTACCTAACGCCACATACAAGTCACGGCTGAAACCCCAAACGATCGCGGCTTCGGGCATCGGCAtttt                         >  1:558968/1‑251 (MQ=255)                                                                                                                                                                                                                  aTACTCGATGATTCTATAAGGGGTTACTGCACAGTTTCTTTACCTAAAATTTTACTAAACCGATAACGGCGGTCGAACAGGCATAATATCCCCCCGAGCACCATAAACAAACCACCAAG‑CCATATCCAGCGGGTAAAGGGGTTGTAATACAAGCGCAACGCCCCGGAATTGTCGCCTAATTTTTTACCTAACGCCACACACAAGTCACGGCTGAAACCCCAATCGATCGCGGCCTCGGTCATCGGCAtttt                         >  1:654459/1‑251 (MQ=255)                                                                                                                                                                                                                     cTCGATGATTCTATAAGGGGTTACTGCACAGTTTCTTTACCTAAAATTTTACTAAACCGATAACGGCGGTCGAACAGGCATAATATCCCGCCGAGCACCATAAACAACCCACCAAG‑CCATATCCAGCGGATAAAGGGTTTGTAATACAAGCGCAACGCCCAGGaa                                                                                                            <  1:247742/165‑1 (MQ=255)                                                                                                                                                                                                                     cTCGATGATTCTATAAGGGGTTACTGCACAGTTTCTTTACCTAAAATTTTACTAAACCGATAACGGCGGTCGAACAGGCATAATATCCCGCCGAGCACCATAAACAACCCACCAAG‑CCATATCCAGCGGATAAAGGGTTTGTAATACAAGCGCAACGCCCAGGAATTGTCGTCTAATTTTTCACCTAACGCCACATACAAGTCACGGc                                                                 <  1:545244/208‑1 (MQ=255)                                                                                                                                                                                                                             ttCTATAAGGGGTTACTGCACAGTTTCTTTACCTAAAATTTTACTAAACCGATAACGGCGGTCGAAAAGGCATAATATCCCGCCGAGCACCCTAAACAACCCACCAAG‑CCATATCCAGCGGATAAAGGGGTTGTAATACAAGCGCAACGCCCAGGGATTGTCGTCTAATTTTTCACCTAACGCCACATACAAGTCACGGCTGAAACCCCAATCGATCGCGGCTTCGGTCATCGGCATTTTTCTCCCGgtgt              >  1:181101/1‑251 (MQ=255)                                                                                                                                                                                                                              gcTATAAGGGGTTACTGCACAGTTTCTTTACCTAAAATTTTACTAAACCGATAACGGCGGTCGAAAAGGCATAATATCCCGCCGAGCACCATAAACAACCCACCAAG‑CCATATCCAGCGGATAAAGGGGTTGTAATACAAGCGCAACGCCCAGGAATTGTCGTCTAATTTTTCACCTAACGCCACATACAAGACACGGCTGAAACCCCAATCGATCGCGGCTTCGGTCATCGGCATTTTGCTCACGGTGta             >  1:230973/2‑251 (MQ=255)                                                                                                                                                                                                                               cTATAAGGGGTTACTGCACAGTTTCTTTACCTAAAATTTTACTAAACCGATAACGGCGGTTGAACAGGCCTAATATCCCGCCGAGCACCATAAACAACCCACCAAG‑CCAAATCCACCGGATAAAGGGTTTGTAATACAAGCGCAACGCCCAGGACTTGTCGTCTAATTTTTTACCTAAAGCCACATAAAAGTCACGGCTGAAACCCCAATTCATCGCGGCTTCGGCCCTCCGCAATTTGCTCAccgcgcat            >  1:106235/1‑244 (MQ=255)                                                                                                                                                                                                                               cTATAAGGGGTTACTGCACAGTTTCTTTACCTAAAATTTTACTAAACCGATAACGGCGGTTGAAAAAGCAAAATATCCCCCCGAGAACCATAAAAAAACCCCCAAGCCCAAACCCA‑CGGGTAAAAGGGTTGGAATAAAAACGCAACCCCCAGGGATTTTCGGCTAATTTTTTACCTAAAGCCCCATACAAATCACGGCTGAAACCCCAAACGAACGCGGCCTCGGGCATCGGCCTTTTGCTCACGGGGtat            >  1:154269/1‑251 (MQ=255)                                                                                                                                                                                                                               cTATAAGGGGTTACTGCACAGTTTCTTTACCTAAAATTTTACTAAACCGATAACGGCGGTCGAACAGGCATAATATCCCGCCGAGCACCCTAAACAACCCACCAAA‑CCCTATCCCGCGGGTAAAGGGTTTGTAATACAAACGCAACGCCCAGGAATTGTCGTCTAATTTTTCACCTAACGCCACATACAAATCACGGCTGAAACCCCAATCGCTCTCGGCTTCCGTCATCGGCCTTTTTCTCACGGGGtat            >  1:829361/1‑251 (MQ=255)                                                                                                                                                                                                                               cTATAAGGGGTTACTGCACAGTTTCTTTACCTAAAATTTTACTAAACCGATAACGGCGGTCGAACAGGCATAATATCCCGCCGAGCACCATAAACAACCCACCAAG‑CCATATCCAGCGGATAAAggg                                                                                                                                        <  1:601431/127‑1 (MQ=255)                                                                                                                                                                                                                               cTATAAGGGGTTACTGCACAGTTTCTTTACCTAAAATTTTACTAAACCGATAACGGCGGTCGAACAGGCAAAATATTCCGCCCAGCACCATAAACAACCCACCAAG‑CCAAATCCAGCGGATAAAGGGGTTGTAAAACAAGCGCAACCCCCCGGAATTGTCGGCCAATTTTTTACCTAAAGCCACCTACAAGACACCGCTGAAACCCCAAACGATCGCCGCTTCCGTTATCGGCATTTTGCTCACCGTGtat            >  1:598420/1‑251 (MQ=255)                                                                                                                                                                                                                                tataAGGGGTTACTGCACAGTTTCTTTACCTAAAATTTTACTAAACCGATAACGGCGGTCGAACAGGCATAatat                                                                                                                                                                                            <  1:872268/75‑1 (MQ=255)                                                                                                                                                                                                                                tataAGGGGTTACTGCACAGTTTCTTTACCTAAAATTTTACTAAACCGATAACGGCGGTCGAACAGGCATAATATCCCGCCGAGCACCATAaac                                                                                                                                                                         <  1:713639/94‑1 (MQ=255)                                                                                                                                                                                                                                    aGGGGTTACTGCACAGTTTCTTTACCTAAAATTTTACTAAACCGATAACGGCGGTCGAACAGGCATAATATCCCGCCGAGCACCATAAACAACCCACCAAG‑CCATATCCAGCGGATAAAGGGTTTGTAATACAAGCGCAACGCCCAGGAATTGTCGTCTAATTTTTCACCTAACGCCACATACAAGTCACGGCTGAAACCCCAATCGATCGCGGCTTCGGTCATCGGCATTTTGCTCACGGTGtata           <  1:200136/247‑1 (MQ=255)                                                                                                                                                                                                                                      gggTTACTGCACAGTTTCTTTACCTAAAATTTTACTAAACCGATAACGGCGGTCGAACAGGGATAATATCCCGCCGAACACCATAAAAAAACCACCAAG‑CCATATCCAGCGGGTAAAAGGGTTGTAATAAAAGCGCAACGCCCAGGGATTGTCGTCTAATTTTTTTACTAACGCCACATACAAATCAccgc                                                                 >  1:253146/1‑188 (MQ=255)                                                                                                                                                                                                                                      gggTTACTGCACAGTTTCTTTACCTAAAATTTTACTAAACCGATAACGGCGGTCGAACAGGCATAATATCCCGCCGAGCACCATAAACAACCCACCAAG‑CCATATCCAGCGGATAAAGGGTTTGTAATACAAGCGCAACGCCCCGGAATTGTCGTCTAATTTTTTACCTAACGCCACATACAAGTCACGGCTGAAACCCCAATCGATCGCGGCTTCGGTCATCGGCCTTTTGCTCACGGTGTATAAGCggt     >  1:12435/1‑249 (MQ=255)                                                                                                                                                                                                                                      gggTTACTGCACAGTTTCTTTACCTAAAATTTTACTAAACCGATAACGGCGGTCGAACAGGCATAATATCCCGCCGAGCACCATAAACAACCCACCAAG‑CCATATCCAGCGGATAAAGGGTTTGTAATACAAGCGCAACGCCCAGGAATTGTCGTCTAATTTTTCACCTAACGCCACATACAAGTCACGGCTGAAACCCCAATCGATCGCGGCTTCGGTCATCGGCATTTTGCTCACGGTGTATAAGCg       <  1:685955/249‑1 (MQ=255)                                                                                                                                                                                                                                        gTTACTGCACAGTTTCTTTACCTAAAATTTTACTAAACCGATAACGGCGGTCGAACAGGCATAATATCCCGCCGAGCACCATAaac                                                                                                                                                                         <  1:177122/86‑1 (MQ=255)                                                                                                                                                                                                                                         ttACTGCACAGTTTCTTTACCTAAAATTTTACTAAACCGATAACGGCGGCAGAACAGGCATAATATTCCCCCGAGCACCATAAACAAACCACCAAG‑CCATATCCAGCGGATAAAGGGCTTGGAAAACAAACGCAACGCCCAGGAAAATACGTCCAATTTTTTACCTAACGGCACATACAAGCCAAGGCTGAAACCCCAATCGGACGCGGCCTCGGTCCTCGGCATATTGCCCACCGTGTTAAAACGttttt  >  1:381727/1‑251 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                   CGGTTTGCCCTGTTTAAACAGCGTTTCATCATAGGTTTTATCCGATAATAAATCCTGCAACGTCTTCGGCGGAACCGGCTTACCCACCAACGCCGATTCCAATGCTTTCGGATCATCACCCAGTGCATTGCGTTGTAATTGCACCAGAAAGGCGGTAACCAAAACGAAGAAAATAATTAAAGGTAAATATAATTTCTTTTTCATTATCAATACTCGATGATTCTATAAGGGGTTACTGCACGGTTTCTTTACCTAAAATTTTACTAAACCGATAACGGCGGTCGAACAGGCATAATATCCCGCCGAGCACCATAAACAACCCACCAAG‑CCATATCCAGCGGATAAAGGGTTTGTAATACAAGCGCAACGCCCAGGAATTGTCGTCTAATTTTTCACCTAACGCCACATACAAGTCACGGCTGAAACCCCAATCGATCGCGGCTTCGGTCATCGGCATTTTGCTCACGGTGTATAAGCGTTTTT  >  1/1995930‑1996412

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑