Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,958,212	A→G	intergenic (‑58469/–)	unknown ← / –	tRNA‑Met‑CAT/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,958,212	0	A	G	100.0%	155.1 / NA	55	intergenic (‑58469/–)	unknown/–	tRNA‑Met‑CAT/–
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (34/21);  total (34/21)

CCGGTTTTAGCCCCACGCATTAAACACTTAGACGACGTGCGCGGTCACTCCCTCATGATCCACGCTGGTGGCGATAACCACTCCGATCACCCGGCCCCACTTGGCGGCGGCGGTCCGCGTATGGCGTGTGGTGTAATTAAATAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCCGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTAAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTAAATTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAG  >  1/1957974‑1958372                                                                                                                                                                                                                                               |                                                                                                                                                                 ccGGTTTTAGCCCCACGCATTAAACACTTAGACGACGTGCGCGGTCACTCCCTCATGATCCACGCTGGTGGCGATAACCACTCCGATCACCCGGCCCCACTTGGCGGCGGCGGTCCGCGTATGGCGTGTGGTGTAATTAAATAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAAc                                                                                                                                                      <  1:493246/251‑1 (MQ=255)                cGCATTAAACACTTAGACGACGTGCGCGGTCACTCCCTCATGATCCACGCTGGTGGCGATAACCACTCCGATCACCCGGCCCCACTTGGCGGCGGCGGTCCGCGTATGGCGTGTGGTGTAATTAAATAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAgg                                                                                                                                       >  1:735912/1‑251 (MQ=255)                  cATTAAACACTTAGACGACGTGCGCGGTCACTCCCTCATGATCCACGCTGGTGGCGATAACCACTCCGATCACCCGGCCCCACTTGGCGGCGGCGGTCCGCGTATGGCGTGTGGTGTAATTAAATAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGAt                                                                                                                                     >  1:732022/1‑251 (MQ=255)                   aTTAAACACTTAGACGACGTGCGCGGTCACTCCCTCATGATCCACGCTGGTGGCGATAACCACTCCGATCACCCGGCCCCACTTGGCGGCGGCGGTCCGCGTATGGCGTGTGGTGTAATTAAATAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGGCACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAg                                                                                                                                                    >  1:645318/1‑235 (MQ=255)                         cacTTAGACGACGTGCGCGGTCACTCCCTCATGATCCACGCTGGTGGCGATAACCACTCCGATCACCCGGCCCCACTTGGCGGCGGCGGTCCGCGTATGGCGTGTGGTGTAATTAAATAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTa                                                                                                                              >  1:790635/1‑251 (MQ=255)                           cTTAGACGACGTGCGCGGTCACTCCCTCATGATCCACGCTGGTGGCGATAACCACTCCGATCACCCGGCCCCACTTGGCGGCGGCGGTCCGCGTATGGCGTGTGGTGTAATTAAATAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTAt                                                                                                                             >  1:11409/1‑250 (MQ=255)                           cTTAGACGACGTGCGCGGTCACTCCCTCATGATCCACGCTGGTGGCGATAACCACTCCGATCACCCGGCCCCACTTGGCGGCGGCGGTCCGCGTATGGCGTGTGGTGTAATTAAATAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTAt                                                                                                                             >  1:93824/1‑250 (MQ=255)                           cTTAGACGACGTGCGCGGTCACTCCCTCATGATCCACGCTGGTGGCGATAACCACTCCGATCACCCGGCCCCACTTGGCGGCGGCGGTCCGCGTATGGCGTGTGGTGTAATTAAATAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTAt                                                                                                                             >  1:237662/1‑250 (MQ=255)                                     gTGCGCGGTCACTCCCTCATGATCCACGCTGGTGGCGATAACCACTCCGATCACCCGGCCCCACTTGGCGGCGGCGGTCCGCGTATGGCGTGTGGTGTAATTAAATAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTa                                                                                                                              <  1:504748/239‑1 (MQ=255)                                          cgGTCACTCCCTCATGATCCACGCTGGTGGCGATAACCACTCCGATCACCCGGCCCCACTTGGCGGCGGCGGTCCGCGTATGGCGTGTGGTGTAATTAAATAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCtgaa                                                                                                             >  1:635018/1‑248 (MQ=255)                                          cgGTCACTCCCTCATGATCCACGCTGGTGGCGATAACCACTCCGATCACCCGGCCCCACTTGGCGGCGGCGGTCCGCGTATGGCGTGTGGTGTAATTAAATAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCtgaa                                                                                                             >  1:631828/1‑248 (MQ=255)                                            gTCACTCCCTCATGATCCACGCTGGTGGCGATAACCACTCCGATCACCCGGCCCCACTTGGCGGCGGCGGTCCGCGTATGGCGTGTGGTGTAATTAAATAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCtgaac                                                                                                            <  1:475827/250‑5 (MQ=255)                                                cTCCCTCATGATCCACGCTGGTGGCGATAACCACTCCGATCACCCGGCCCCACTTGGCGGCGGCGGTCCGCGTATGGCGTGTGGTGTAATTAAATAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCCAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTtcat                                                                                                       >  1:514412/1‑251 (MQ=21)                                                  cccTCATGATCCACGCTGGTGGCGATAACCACTCCGATCACCCGGCCCCACTTGGCGGCGGCGGTCCGCGTATGGCGTGTGGTGTAATTAAATAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTtcatca                                                                                                     >  1:555863/1‑251 (MQ=255)                                                   ccTCATGATCCACGCTGGTGGCGATAACCACTCCGATCACCCGGCCCCACTTGGCGGCGGCGGTCCGCGTATGGCGTGTGGTGTAATTAAATAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTTCATCAg                                                                                                    <  1:134948/251‑1 (MQ=255)                                                    cTCATGATCCACGCTGGTGGCGATAACCACTCCGATCACCCGGCCCCACTTGGCGGCGGCGGTCTGCGTATGGCGTGTGGTGTAATTAAATAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGt                                                                                                                    <  1:434363/234‑1 (MQ=255)                                                    cTCATGATCCACGCTGGTGGCGATAACCACTCCGATCACCCGGCCCCACTTGGCGGCGGCGGTCCGCGTATGGCGTGTGGTGTAATTAAATAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGt                                                                                                                    >  1:592600/1‑234 (MQ=255)                                                         gATCCACGCTGGTGGCGATAACCACTCCGATCACCCGGCCCCACTTGGCGGCGGCGGTCCGCGTATGGCGTGTGGTGTAATTAAATAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTg                                                                                                                        >  1:591187/1‑225 (MQ=255)                                                          aTCCACGCTGGTGGCGATAACCACTCCGATCACCCGGCCCCACTTGGCGGCGGCGGTCCGCGTATGGCGTGTGGTGTAATTAAATAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTTCATCAGATTtct                                                                                              >  1:846594/1‑250 (MQ=255)                                                           tCCACGCTGGTGGCGATAACCACTCCGATCACCCGGCCCCACTTGGCGGCGGCGGTCCGCGTATGGCGTGTGGTGTAATTAAATAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTTCATCAGATTTCTCg                                                                                            <  1:443709/251‑1 (MQ=255)                                                                    gtggCGATAACCACTCCGATCACCCGGCCCCACTTGGCGGCGGCGGTCCGCGTATGGCGTGTGGTGTAATTAAATAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTTCATCAGATTTCTCGTTCTCAAg                                                                                    <  1:105708/250‑1 (MQ=255)                                                                      ggCGATAACCACTCCGATCACCCGGCCCCACTTGGCGGCGGCGGTCCGCGTATGGCGTGTGGTGTAATTAAATAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAAc                                                                                                                                                      >  1:792451/1‑182 (MQ=255)                                                                      ggCGATAACCACTCCGATCACCCGGCCCCACTTGGCGGCGGCGGTCCGCGTATGGCGTGTGGTGTAATTAAATAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTtcatc                                                                                                      <  1:737475/230‑1 (MQ=18)                                                                           tAACCACTCCGATCACCCGGCCCCACTTGGCGGCGGCGGTCCGCGTATGGCGTGTGGTGTAATTAAATAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAgg                                                                                                                     >  1:156129/1‑210 (MQ=255)                                                                           tAACCACTCCGATCACCCGGCCCCACTTGGCGGCGGCGGTCCGCGTATGGCGTGTGGTGTAATTAAATAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTTCATCAGATTTCTCGTTCTCAAGTCATTTAt                                                                            <  1:711632/251‑1 (MQ=255)                                                                           tAACCACTCCGATCACCCGGCCCCACTTGGCGGCGGCGGTCCGCGTATGGCGTGTGGCGTAATTAAATAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTTCATCAGCTTTCTCGTTCTCAAGTCATTTAt                                                                            >  1:566099/1‑251 (MQ=18)                                                                                    cGATCACCCGGCCCCACTTGGCGGCGGCGGTCCGCGTATGGCGTGTGGTGTAATTAAATAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAAGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAg                                                                    >  1:438090/1‑250 (MQ=18)                                                                                         aCCCGGCCCCACTTGGCGGCGGCGGTCCGCGTATGGCGTGTGGTGTAATTAAATAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCttt                                                               >  1:746415/1‑250 (MQ=255)                                                                                              gCCCCACTTGGCGGCGGCGGTCCGCGTATGGCGTGTGGTGTAATTAAATAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGaa                                                         >  1:34443/1‑251 (MQ=255)                                                                                                cccACTTGGCGGCGGCGGTCCGCGTATGGCGTGTGGTGTAATTAAATAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACTCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAag                                                       <  1:445159/251‑1 (MQ=17)                                                                                                  cACTTGGCGGCGGCGGTCCGCGTATGGCGTGTGGTGTAATTAAATAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAagag                                                     <  1:637682/251‑1 (MQ=255)                                                                                                         cggcggcggTCCGCGTATGGCGTGTGGTGTAATTAAATAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCtgaact                                                                                                           <  1:518583/190‑6 (MQ=17)                                                                                                           gcggcggTCCGCGTATGGCGTGTGGTGTAATTAAATAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAAt                                             <  1:675978/250‑1 (MQ=255)                                                                                                                    cgcgTATGGCGTGTGGTGTAATTAAATAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAAt                                   <  1:661990/251‑1 (MQ=255)                                                                                                                     gcgTATGGCGTGTGGTGTAATTAAATAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAAt                                             <  1:303672/240‑1 (MQ=14)                                                                                                                      cgTATGGCGTGTGGTGTAATTAAATAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTaaaa                                    >  1:226559/1‑248 (MQ=255)                                                                                                                      cgTATGGCGTGTGGTGTAATTAAATAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATc                                  >  1:624914/1‑250 (MQ=255)                                                                                                                           ggCGTGTGGTGTAATTAAATAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTta                            >  1:870789/1‑251 (MQ=255)                                                                                                                           ggCGTGTGGTGTAATTAAATAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTta                            >  1:683248/1‑251 (MQ=255)                                                                                                                           ggCGTGTGGTGTAATTAAATAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTta                            >  1:755968/1‑251 (MQ=255)                                                                                                                              gtgtGGTGTAATTAAATAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATgg                                           >  1:613573/1‑233 (MQ=14)                                                                                                                              gtgtGGTGTAATTAAATAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTtatat                         >  1:429341/1‑251 (MQ=255)                                                                                                                                  ggtgTAATTAAATAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTTATATaaa                      >  1:499357/1‑250 (MQ=255)                                                                                                                                   gtgtAATTAAATAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTg                                                                          >  1:296642/1‑197 (MQ=14)                                                                                                                                   gtgtAATTAAATAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTtatat                         >  1:194923/1‑246 (MQ=255)                                                                                                                                     gtAATTAAATAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAg                                                                                                                                                    >  1:524268/1‑121 (MQ=14)                                                                                                                                         ttAAATAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTTATATAAACCCTCAAc              <  1:264823/251‑1 (MQ=255)                                                                                                                                          tAAATAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTTATATAAACCCTCAACg             <  1:189453/251‑1 (MQ=255)                                                                                                                                           aaaTAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGt                                                                                                                               >  1:666057/1‑136 (MQ=11)                                                                                                                                           aaaTAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTTATATAAACCCTCAACg             >  1:377928/1‑250 (MQ=255)                                                                                                                                             ataatCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTTATATAAACCCTCAACGcc           >  1:4314/1‑250 (MQ=255)                                                                                                                                                   cGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTTATATAAACCCTCAACGCCCTAAAAt    <  1:768512/251‑1 (MQ=255)                                                                                                                                                   cGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTTATATAAACCCTCAACGCCCTAAAAt    <  1:456693/251‑1 (MQ=255)                                                                                                                                                     cTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAg  <  1:731158/251‑1 (MQ=255)                                                                                                                                                     cTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAg  <  1:327616/251‑1 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                 CCGGTTTTAGCCCCACGCATTAAACACTTAGACGACGTGCGCGGTCACTCCCTCATGATCCACGCTGGTGGCGATAACCACTCCGATCACCCGGCCCCACTTGGCGGCGGCGGTCCGCGTATGGCGTGTGGTGTAATTAAATAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCCGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTAAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTAAATTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAG  >  1/1957974‑1958372

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑