Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,908,466	A→G	intergenic (‑8723/–)	unknown ← / –	tRNA‑Met‑CAT/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,908,466	0	A	G	100.0%	234.3 / NA	78	intergenic (‑8723/–)	unknown/–	tRNA‑Met‑CAT/–
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (42/36);  total (42/36)

GAAAGTTTTGTTTGCCGCCGTCATCATGGGATTGTTGGTTTGGTACAACAGCCCAAGCCTGACGGAATGGAACGATATGCGCTTTCTCACCCGTGTACATTGGCTGACGTGGCTCATCGTGTTGGCAGCGATGGTTTACGGTGGCATGTTGGTGCTATTGGGGATTCGCAAACGTGATTTGACGGGCGCGCACTCCGCGTGAAATTAGTTTTTGTGGCGCATGGATTTATGCGTATAATACCGAAGTATTTTTTCACCGAAATTTATGGAACACATTGAATGCAGCTGATTCGAGGCCTGGGAAATTTACCGCAAAATTTGCACGCTTGCGCGTTAACCATCGGTAATTTTGATGGCGTGCATCTGGGGCATCAAGCTATTTTGCGTCATTTGCGGGAAAAAGCCGACGAATTGCATTTGCCCATGGTGGTGATGCTGTTTGAACCGCAGCCGCGTGAATATTTTTGCGGTGAAGATGCGCCG  >  1/1908227‑1908709                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                    gAAAGTTTTGTTTGCCGCCGTCATCATGGGATTGTTGGTTTGGTACAACAGCCCAAGCCTGACGGAATGGAACGATATGCGCTTTCTCACCCGTGTACATTGGCTGACGTGGCTCATCGTGTTGGCAGCGATGGTTTACGGTGGCATGTTGGTGCTGTTGGGGATTCGCAAACGTGATTTGACGGGCGCGCACTCCGCGTGAAATTAGTTTTTGTGGCGCATGGATTTATGCGTATAATGCCGAAGTAttt                                                                                                                                                                                                                                          >  1:674508/1‑251 (MQ=255)      ttttGTTTGCCGCCGTCATCATGGGATTGTTGGTTTGGTACAACAGCCCAAGCCTGACGGAATGGAACGATATGCGCTTTCTCACCCGTGTACATTGGCTGACGTGGCTCATCGTGTTGGCAGCGATGGTTTACGGTGGCATGTTGGTGCTGTTGGGGATTCGCAAACGTGATTTGACGGGCGCGCACTCCGCGTGAAATTAGTTTTTGTGGCGCATGGATTTATGCGTATAATGCCGAAGTAtttttt                                                                                                                                                                                                                                       <  1:772378/249‑1 (MQ=255)      ttttGTTTGCCGCCGTCATCATGGGATTGTTGGTTTGGTACAACAGCCCAAGCCTGACGGAATGGAACGATATGCGCTTTCTCACCCGTGTACATTGGCTGACGTGGCTCATCGTGTTGGCAGCGATGGTTTACGGTGGCATGTTGGTGCTGTTGGGGATTCGCAAACGTGATTTGACGGGCGCGCACTCCGCGTGAAATTAGTTTTTGTGGCGCATGGATTTATGCGTATAATGCCGAAGTATTTTTTc                                                                                                                                                                                                                                      <  1:716375/250‑1 (MQ=255)             tgCCGCCGTCATCATGGGATTGTTGGTTTGGTACAACAGCCCAAGCCTGACGGAATGGAACGATATGCGCTTTCTCACCCGTGTACATTGGCTGACGTGGCTCATCGTGTTGGCAGCGATGGTTTACGGTGGCATGTTGGTGCTGTTGGGGATTCGCAAACGTGATTTGACGGGCGCGCACTCCGCGTGAAATTAGTTTTTGTGGCGCATGGATTTATGCGTATAATGCCGAAGTATTTTTTCACCGAAAt                                                                                                                                                                                                                              <  1:141568/251‑1 (MQ=255)                  ccgTCATCATGGGATTGTTGGTTTGGTACAACAGCCCAAGCCTGACGGAATGGAACGATATGCGCTTTCTCACCCGTGTACATTGGCTGACGTGGCTCATCGTGTTGGCAGCGATGGTTTACGGTGGCATGTTGGTGCTGTTGGGGATTCGCAAACGTGATTTGACGGGCGCGCACTCCGCGTGAAATTAGTTTTTGTGGCGCATGGATTTATGCGTATAATGCCGAAGTATTTTTTCACCGAAATTTAt                                                                                                                                                                                                                          <  1:51790/250‑1 (MQ=255)                      catcatGGGATTGTTGGTTTGGTACAACAGCCCAAGCCTGACGGAATGGAACGATATGCGCTTTCTCACCCGTGTACATTGGCTGACGTGGCTCATCGTGTTGGCAGCGATGGTTTACGGTGGCATGTTGGTGCTGTTGGGGATTCGCAAACGTGATTTGACGGGCGCGCACTCCGCGTGAAATTAGTTTTTGTGGCGCATGGATTTATGCGTATAATGCCGAAGTATTTTTTCACCGAAATTTATGGAac                                                                                                                                                                                                                     <  1:177148/251‑1 (MQ=255)                        tcatGGGATTGTTGGTTTGGTACAACAGCCCAAGCCTGACGGAATGGAACGATATGCGCTTTCTCACCCGTGTACATTGGCTGACGTGGCTCATCGTGTTGGCAGCGATGGTTTACGGTGGCATGTTGGTGCTGTTGGGGATTCGCAAACGTGATTTGACGGGCGCGCACTCCGCGTGAAATTAGTTTTTGTGGCGCATGGATTTATGCGTATAATGCCGAAGTATTTTTTCACCGAAATTTATGGAaca                                                                                                                                                                                                                    >  1:567521/1‑250 (MQ=255)                        tcatGGGATTGTTGGTTTGGTACAACAGCCCAAGCCTGACGGAATGGAACGATATGCGCTTTCTCACCCGTGTACATTGGCTGACGTGGCTCATCGTGTTGGCAGCGATGGTTTACGGTGGCATGTTGGTGCTGTTGAGGATTCGCAAACGTGATTTGACGGGCGCGCACTCCGCGTGAAATTAGTTTTTGTGGCGCATGGATTTATGCGTATAATGCCGAAGTATTTTTTCACCGAAATTTATGGAaca                                                                                                                                                                                                                    >  1:120178/1‑250 (MQ=255)                                 tgttgGTTTGGTACAACAGCCCAAGCCTGACGGAATGGAACGATATGCGCTTTCTCACCCGTGTACATTGGCTGACGTGGCTCATCGTGTTGGCAGCGATGGTTTACGGTGGCATGTTGGTGCTGTTGGGGATTCGCAAACGTGATTTGACGGGCGCGCACTCCGCGTGAAATTAGTTTTTGTGGCGCATGGATTTATGCGTATAATGCCGAAGTATTTTTTCACCGAAATTTATGGAACACATTGAATGc                                                                                                                                                                                                          <  1:1881/251‑1 (MQ=255)                                         tggtACAACAGCCCAAGCCTGACGGAATGGAACGATATGCGCTTTCTCACCCGTGTACATTGGCTGACGTGGCTCATCGTGTTGGCAGCGATGGTTTACGGTGGCATGTTGGTGCTGTTGGGGATTCGCAAACGTGATTTGACGGGCGCGCACTCCGCGTGAAATTAGTTTTTGTGGCGCATGGATTTATGCGTATAATGCCGAAGTATTTTTTCACCGAAATTTATGGAac                                                                                                                                                                                                                     >  1:144860/1‑232 (MQ=255)                                         tggtACAACAGCCCAAGCCTGACGGAATGGAACGATATGCGCTTTCTCACCCGTGTACATTGGCTGACGTGGCTCATCGTGTTGGCAGCGATGGTTTACGGTGGCATGTTGGTGCTGTTGGGGATTCGCAAACGTGATTTGACGGGCGCGCACTCCGCGTGAAATTAGTTTTTGTGGCGCATGGATTTATGCGTATAATGCCGAAGTATTTTTTCACCGAAATTTATGGAACACATTGAATGCAGCTGAtt                                                                                                                                                                                                  >  1:290199/1‑251 (MQ=255)                                          ggtACAACAGCCCAAGCCTGACGGAATGGAACGATATGCGCTTTCTCACCCGTGTACATTGGCTGACGTGGCTCATCGTGTTGGCAGCGATGGTTTACGGTGGCATGTTGGTGCTGTTGGGGATTCGCAAACGTGATTTGACGGGCGCGCACTCCGCGTGAAATTAGTTTTTGTGGCGCATGGATTTATGCGTATAATGCCGAAGTATTTTTTCACCGAAATTTATGGAACACATTGAATGCAGCTGAtt                                                                                                                                                                                                  >  1:410918/1‑250 (MQ=255)                                          ggtACAACAGCCCAAGCCTGACGGAATGGAACGATATGCGCTTTCTCACCCGTGTACATTGGCTGACGTGGCTCATCGTGTTGGCAGCGATGGTTTACGGTGGCATGTTGGTGCTGTTGGGGATTCGCAAACGTGATTTGACGGGCGCGCACTCCGCGTGAAATTAGTTTTTGTGGCGCATGGATTTATGCGTATAATGCCGAAGTATTTTTTCACCGAAATTTATGGAACACATTGAATGCAGCTGAtt                                                                                                                                                                                                  >  1:57865/1‑250 (MQ=255)                                           gtACAACAGCCCAAGCCTGACGGAATGGAACGATATGCGCTTTCTCACCCGTGTACATTGGCTGACGTGGCTCATCGTGTTGGCAGCGATGGTTTACGGTGGCATGTTGGTGCTGTTGGGGATTCGCAAACGTGATTTGACGGGCGCGCACTCCGCGTGAAATTAGTTTTTGTGGCGCATGGATTTATGCGTATAATGCCGAAGTATTTTTTCACCGAAATTTATg                                                                                                                                                                                                                         <  1:528840/226‑1 (MQ=255)                                            tACAACAGCCCAAGCCTGACGGAATGGAACGATATGCGCTTTCTCACCCGTGTACATTGGCTGACGTGGCTCATCGTGTTGGCAGCGATGGTTTACGGTGGCATGTTGGTGCTGTTGGGGATTCGCAAACGTGATTTGACGGGCGCGCACTCCGCGTGAAATTAGTTTTTGTGGCGCATGGATTTATGCGTATAATGCCGAAGTATTTTTTCACCGAAATTTATGGAACACATTGAATGCAGCTGATTCg                                                                                                                                                                                                <  1:2855/250‑1 (MQ=255)                                              caacaGCCCAAGCCTGACGGAATGGAACGATATGCGCTTTCTCACCCGTGTACATTGGCTGACGTGGCTCATCGTGTTGGCAGCGATGGTTTACGGTGGCATGTTGGTGCTGTTGGGGATTCGCAAACGTGATTTGACGGGCGCGCACTCCGCGTGAAATTAGTTTTTGTGGCGCATGGATTTATGCGTATAATGCCGAAGTATTTTTTCACCGAAATTTATGGAACACATTGAATGCAGCTGATTCGAgg                                                                                                                                                                                             <  1:416757/251‑1 (MQ=255)                                                   gCCCAAGCCTGACGGAATGGAACGATATGCGCTTTCTCACCCGTGTACATTGGCTGACGTGGCTCATCGTGTTGGCAGCGATGGTTTACGGTGGCATGTTGGTGCTGTTGGGGATTCGCAAACGTGATTTGACGGGCGCGCACTCCGCGTGAAATTAGTTTTTGTGGCGCATGGATTTATGCGTATAATGCCGAAGTATTTTTTCACCGAAATTTATGGAACACATTGAATGc                                                                                                                                                                                                          <  1:746613/233‑1 (MQ=255)                                                         gCCTGACGGAATGGAACGATATGCGCTTTCTCACCCGTGTACATTGGCTGACGTGGCTCATCGTGTTGGCAGCGATGGTTTACGGTGGCATGTTGGTGCTGTTGGGGATTCGCAAACGTGATTTGACGGGCGCGCACTCCGCGTGAAATTAGTTTTTGTGGCGCATGGATTTATGCGTATAATGCCGAAGTATTTTTTCACCGAAATTTATGGAACACATTGAATGCAGCTGATTCGAGGCCTGGGAAAtt                                                                                                                                                                                  >  1:474765/1‑251 (MQ=255)                                                                         cGATATGCGCTTTCTCACCCGTGTACATTGGCTGACGTGGCTCATCGTGTTGGCAGCGATGGTTTACGGTGGCATGTTGGTGCTGTTGGGGATTCGCAAACGTGATTTGACGGGCGCGCACTCCGCGTGAAATTAGTTTTTGTGGCGCATGGATTTATGCGTATAATGCCGAAGTATTTTTTCACCGAAATTTATGGAACACATTGAATGCAGCTGATTCGAGGCCTGGGAAATTTACCGCAAAATTTGCa                                                                                                                                                                  >  1:152167/1‑251 (MQ=255)                                                                          gATATGCGCTTTCTCACCCGTGTACATTGGCTGACGTGGCTCATCGTGTTGGCAGCGATGGTTTACGGTGGCATGTTGGTGCTGTTGGGGATTCGCAAACGTGATTTGACGGGCGCGCACTCCGCGTGAAATTAGTTTTTGTGGCGCATGGATTTATGCGTATAATGCCGAAGTATTTTTTCACCGAAATTTATGGAACATATTGAATGCAGCTGATTCGAGGCCTGGGAAATTTACCGCAAAATTTGCa                                                                                                                                                                  >  1:225351/1‑250 (MQ=255)                                                                           atatGCGCTTTCTCACCCGTGTACATTGGCTGACGTGGCTCATCGTGTTGGCAGCGATGGTTTACGGTGGCATGTTGGTGCTGTTGGGGATTCGCAAACGTGATTTGACGGGCGCGCACTCCGCGTGAAATTAGTTTTTGTGGCGCATGGATTTATGCGTATAATGCCGAAGTATTTTTTCACCGAAATTTATGGAACACATTGAATGCAGCTGATTCGAGGCCTGGGAAATTTACCGCAAAATTTGCACg                                                                                                                                                                >  1:123472/1‑251 (MQ=255)                                                                                    ttCTCACCCGTGTACATTGGCTGACGTGGCTCATCGTGTTGGCAGCGATGGTTTACGGTGGCATGTTGGTGTTGTTGGGGATTCGCAAACGTGATTTGACGGGCGCGCACTCCGCGTGAAATTAGTTTTTGTGGCGCATGGATTTATGCGTATAATGCCGAAGTATTTTTTCACTGAAATTTATGGAACAAATTGAATGCAGCTGATTCGAGGCCTGGGAAATTTACCGCAAAATTTGCACGCTTGCGCGt                                                                                                                                                       >  1:31601/1‑251 (MQ=255)                                                                                    ttCTCACCCGTGTACATTGGCTGACGTGGCTCATCGTGTTGGCAGCGATGGTTTACGGTGGCATGTTGGTGCTGTTGGGGATTCGCAAACGTGATTTGACGGGCGCGCACTCCGCGTGAAATTAGTTTTTGTGGCGCATGGATTTATGCGTATAATGCCGAAGTATTTTTTCACCGAAATTTATGGAACACATTGAATGCAGCTGATTCGAGGCCTGGGAAATTTACCGCAAAATTTGCACGCTTGCGCGt                                                                                                                                                       <  1:94178/251‑1 (MQ=255)                                                                                        cACCCGTGTACATTGGCTGACGTGGCTCATCGTGTTGGCAGCGATGGTTTACGGTGGCATGTTGGTGCTGTTGGGGATTCGCAAACGTGATTTGACGGGCGCGCACTCCGCGTGAAATTAGTTTTTGTGGCGCATGGATTTATGCGTATAATGCCGAAGTATTTTTTCACCGAAATTTATGGAACACATTGAATGCAGCTGATTCGAGGCCTGGGAAATTTACCGCAAAATTTGCACGCTTGCGCGTTAAc                                                                                                                                                   <  1:46243/251‑1 (MQ=255)                                                                                         aCCCGTGTACATTGGCTGACGTGGCTCATCGTGTTGGCAGCGATGGTTTACGGTGGCATGTTGGTGCTGTTGGGGATTCGCAAACGTGATTTGACGGGCGCGCACTCCGCGTGAAATTAGTTTTTGTGGCGCATGGATTTATGCGTATAATGCCGAAGTATTTTTTCACCGAAATTTATGGAACACATTGAATGCAGCTGATTCGAGGCCTGGGAAATTTACCGCAAAATTTGCACGCTTGCGCGTTAAcc                                                                                                                                                  <  1:67323/251‑1 (MQ=255)                                                                                          cccGTGTACATTGGCTGACGTGGCTCATCGTGTTGGCAGCGATGGTTTACGGTGGCATGTTGGTGCTGTTGGGGATTCGCAAACGTGATTTGACGGGCGCGCACTCCGCGTGAAATTAGTTTTTGTGGCGCATGGATTTATGCGTATAATGCCGAAGTa                                                                                                                                                                                                                                             >  1:500803/1‑159 (MQ=255)                                                                                          cccGTGTACATTGGCTGACGTGGCTCATCGTGTTGGCAGCGATGGTTTACGGTGGCATGTTGGTGCTGTTGGGGATTCGCAAACGTGATTTGACGGGCGCGCACTCCGCGTGAAATTAGTTTTTGTGGCGCATGGATTTATGCGTATAATGCCGAAGTATTTTTTCACCGAAATTTATGGAACACATTGAATGCAGCTGATTCGAGGCCTGGGAAATTTACCGCAAAATTTGCACGCTTGCGCGTTAACCa                                                                                                                                                 >  1:76263/1‑251 (MQ=255)                                                                                          cccGTGTACATTAGCTGACGTGGCTCATCGTGTTGGCAGCGATGGTTTACGGTGGCATGTTGGTGCTGTTGGGGATTCGCAAACGTGATTTGACGGGCGCGCACTCCGCGTGAAATTAGTTTTTGTGGCGCATGGATTTATGCGTATAATGCCGAAGTATTTTTTCACCGAAATTTATGGAACACATTGAATGCAGCTGATTCGAGGCCTGGGAAATTTACCGCAAAATTTGCACGCTTGCGCGTTAACCa                                                                                                                                                 >  1:719827/1‑251 (MQ=255)                                                                                            cGTGTACATTGGCTGACGTGGCTCATCGTGTTGGCAGCGATGGTTTACGGTGGCATGTTGGTGCTGTTGGGGATTCGCAAACGTGATTTGACGGGCGCGCACTCCGCGTGAAATTAGTTTTTGTGGCGCATGGATTTATGCGTATAATGCCGAAGTATTTTTTCACCGAAATTTATgg                                                                                                                                                                                                                        >  1:647289/1‑178 (MQ=255)                                                                                                      ggCTGACGTGGCTCATCGTGTTGGCAGCGATGGTTTACGGTGGCATGTTGGTGCTGTTGGGGATTCGCAAACGTGATTTGACGGGCGCGCACTCCGCGTGAAATTAGTTTTTGTGGCGCATGGATTTATGCGTATAATGCCGAAGTATTTTTTCACCGAAATTTATGGAACACATTGAATGCAGCTGATTCGAGGCCTGGGAAATTTACCGCAAAATTTGCACGCTTGCGCGTTAACCATCGGTAATTTTg                                                                                                                                     >  1:568719/1‑251 (MQ=255)                                                                                                      ggCTGACGTGGCTCATCGTGTTGGCAGCGATGGTTTACGGTGGCATGTTGGTGCTGTTGGGGATTCGCAAACGTGATTTGACGGGCGCGCACTCCGCGTGAAATTAGTTTTTGTGGCGCATGGATTTATGCGTATAATGCCGAAGTATTTTTTCACCGAAATTTATGGAACACATTGAATGCAGCTGATTCGAGGCCTGGGAAATTTACCGCAAAATTTGCACGCTTGCGCGTTAACCATCGGTAATTTTg                                                                                                                                     >  1:513700/1‑251 (MQ=255)                                                                                                        cTGACGTGGCTCATCGTGTTGGCAGCGATGGTTTACGGTGGCATGTTGGTGCTGTTGGGGATTCGCAAACGTGATTTGACGGGCGCGCACTCCGCGTGAAATTAGTTTTTGTGGCGCATGGATTTATGCGTATAATGCCGAAGTATTTTTTCACCGAAATTTATGGAACACATTGAATGCAGCTGATTCGAGGCCTGGGAAATTTACCGCAAAATTTGCACGCTTGCGCGTTAACCATCGGTAATTTTGAt                                                                                                                                   <  1:126848/251‑1 (MQ=255)                                                                                                           aCGTGGCTCATCGTGTTGGCAGCGATGGTTTACGGTGGCATGTTGGTGCTGTTGGGGATTCGCAAACGTGATTTGACGGGCGCGCACTCCGCGTGAAATTAGTTTTTGTGGCGCATGGATTTATGCGTATAATGCCGAAGTATTTTTTCACCGAAATTTATGGAACACATTGAATGCAGCTGATTCGAGGCCTGGGAAATTTACCGCAAAATTTGCACGCTTGCGCGTTAACCATCGGTAATTTTGATgg                                                                                                                                 >  1:571103/1‑250 (MQ=255)                                                                                                           aCGTGGCTCATCGTGTTGGCAGCGATGGTTTACGGTGGCATGTTGGTGCTGTTGGGGATTCGCAAACGTGATTTGACGGGCGCGCACTCCGCGTGAAATTAGTTTTTGTGGCGCATGGATTTATGCGTATAATGCCGAAGTATTTTTTCACCGAAATTTATGGAACACATTGAATGCAGCTGATTCGAGGCCTGGGAAATTTACCGCAAAATTTGCACGCTTGCGCGTTAACCATCGGTAATTTTGATGGc                                                                                                                                <  1:246896/251‑1 (MQ=255)                                                                                                                 cTCATCGTGTTGGCAGCGATGGTTTACGGTGGCATGTTGGTGCTGTTGGGGATTCGCAAACGTGATTTGACGGGCGCGCACTCCGCGTGAAATTAGTTTTTGTGGCGCATGGATTTATGCGTATAATGCCGAAGTATTTTTTCACCGAAATTTATGGAACACATTGAATACAGCTGATTCGAGGCCTGGGAAATTTACCGCAAAATTTGCACGCTTGCGCGTTAACCATCGGTAATTTTGATGGCGTGCAt                                                                                                                          >  1:429350/1‑251 (MQ=255)                                                                                                                    aTCGTGTTGGCAGCGATGGTTTACGGTGGCATGTTGGTGCTGTTGGGGATTCGCAAACGTGATTTGACGGGCGCGCACTCCGCGTGAAATTAGTTTTTGTGGCGCATGGATTTATGCGTATAATGCCGAAGTATTTTTTCACCGAAATTTATGGAACACATTGAATGCAGCTGATTCGAGGCCTGGGAAATTTACCGCAAAATTTGCACGCTTGCGCGTTAACCATCGGTAATTTTGATGGCGTGCATCTg                                                                                                                       <  1:106191/251‑1 (MQ=255)                                                                                                                      cGTGTTGGCAGCGATGGTTTACGGTGGCATGTTGGTGCTGTTGGGGATTCGCAAACGTGATTTGACGGGCGCGCACTCCGCGTGAAATTAGTTTTTGTGGCGCATGGATTTATGCGTATAATGCCGAAg                                                                                                                                                                                                                                               >  1:238867/1‑129 (MQ=255)                                                                                                                        tgtTGGCAGCGATGGTTTACGGTGGCATGTTGGTGCTGTTGGGGATTCGCAAACGTGATTTGACGGGCGCGCACTCCGCGTGAAATTAGTTTTTGTGGCGCATGGATTTATGCGTATAATGCCGAAGTATTTTTTCACCGAAATTTATGGAACACATTGAATGCAGCTGATTCGAGGCCTGGGAAAttt                                                                                                                                                                                 <  1:735557/189‑1 (MQ=255)                                                                                                                                    tGGTTTACGGTGGCATGTTGGTGCTGTTGGGGATTCGCAAACGTGATTTGACGGGCGCGCACTCCGCGTGAAATTAGTTTTTGTGGCGCATGGATTTATGCGTATAATGCCGAAGTATTTTTTCACCGAAATTTATGGAACACATTGAATGCAGCTGATTCGAGGCCTGGGAAATTTACCGCAAAATTTGCACGCTTGCGCGTTAACCATCGGTAATTTTGATGGCGTGCATCTGGGGCATCAAGCTAttt                                                                                                       <  1:626992/251‑1 (MQ=255)                                                                                                                                    tGGTTTACGGTGGCATGTTGGTGCTGTTGGGGATTCGCAAACGTGATTTGACGGGCGCGCACTCCGCGTGAAATTAGTTTTTGTGGCGCATGGATTTATGCGTATAATGCCGAAGTATTTTTTCACCGAAATTTATGGAACACATTGAATGCAGCTGATTCGAGGCCTGGGAAATTTACCGCAAAATTTGCACGCTTGCGCGTTAACCATCGGTAATTTTGATGGCGTGCATCTGGGGCATCAAGCTAttt                                                                                                       <  1:99390/251‑1 (MQ=255)                                                                                                                                     ggTTTACGGTGGCATGTTGGTGCTGTTGGGGATTCGCAAACGTGATTTGACGGGCGCGCACTCCGCGTGAAATTAGTTTTTGTGGCGCATGGATTTATGCGTATAATGCCGAAGTATTTTTTCACCGAAATTTATGGAACACATTGAATGCAGCTGATTCGAGGCCTGGGAAATTTACCGCAAAATTTGCACGCTTGCGCGt                                                                                                                                                       <  1:280713/202‑1 (MQ=255)                                                                                                                                     ggTTTACGGTGGCATGTTGGTGCTGTTGGGGATTCGCAAACGTGATTTGACGGGCGCGCACTCCGCGTGAAATTAGTTTTTGTGGCGCATGGATTTATGCGTATAATGCCGAAGTATTTTTTCACCGAAATTTATGGAACACATTGAATGCAGCTGATTCGAGGCCTGGGAAATTTACCGCAAAATTTGCACGCTTGCGCGt                                                                                                                                                       >  1:607844/1‑202 (MQ=255)                                                                                                                                      gTTTACGGTGGCATGTTGGTGCTGTTGGGGATTCGCAAACGTGATTTGACGGGCGCGCACTCCGCGTGAAATTAGTTTTTGTGGCGCATGGATTTATGCGTATAATGCCGAAGTATTTTTTCACCGAAATTTATGGAACACATTGAATGCAGCTGATTCGAGGCCTGGGAAATTTACCGCAAAATTTGCACGCTTGCGCGTTAACCATCGGTAATTTTGATGGCGTGCGTCTGGGGCATCAAGCTATTTTg                                                                                                     >  1:597775/1‑251 (MQ=255)                                                                                                                                      gTTTACGGTGGCATGTTGGTGCTGTTGGGGATTCGCAAACGTGATTTGACGGGCGCGCACTCCGCGTGAAATTAGTTTTTGTGGCGCATGGATTTATGCGTATAATGCCGAAGTATTTTTTCACCGAAATTTATGGAACACATTGAATGCAGCTGATTCGAGGCCTGGGAAATTTACCGCAAAATTTGCACGCTTGCGCGTTAACCATCGGTAATTTTGATGGCGTGCATCTGGGGCATCAAGCTATTTTg                                                                                                     >  1:881925/1‑251 (MQ=255)                                                                                                                                      gTTTACGGTGGCATGTTGGTGCTGTTGGGGATTCGCAAACGTGATTTGACGGGCGCGCACTCCGCGTGAAATTAGTTTTTGTGGCGCATGGATTTATGCGTATAATGCCGAAGTATTTTTTCACCGAAATTTATGGAACACATTGAATGCAGCTGATTCGAGGCCTGGGAAATTTACCGCAAAATTTGCACGCTTGCGCGTTAACCATCGGTAATTTTGATGGCGTGCATCTGGGGCATCAAGCTATTTTg                                                                                                     >  1:416020/1‑251 (MQ=255)                                                                                                                                             gTGGCATGTTGGTGCTGTTGGGGATTCGCAAACGTGATTTGACGGGCGCGCACTCCGCGTGAAATTAGTTTTTGTGGCGCATGGATTTATGCGTATAATGCCGAGGTATTTTTTCACCGAAATTTATGGAACACATTGAATGCAGCTGATTCGAGGCCTGGGAAATTTACCGCAAAATTTGCACGCTTGCGCGTTAACCATCGGTAATTTTGATGGCGTGCATCTGGGGCATCAAGCTATTTTGCGTCAtt                                                                                              >  1:818306/1‑251 (MQ=255)                                                                                                                                                   tGTTGGTGCTGTTGGGGATTCGCAAACGTGATTTGACGGGCGCGCACTCCGCGTGAAATTAGTTTTTGTGGCGCATGGATTTATGCGTATAATGCCGAAGTATTTTTTCACCGAAATTTATGGAACACATTGAATGCAGCTGATTCGAGGCCTGGGAAATTTACCGCAAAATTTGCACGCTTGCGCGTTAACCATCGGTa                                                                                                                                           <  1:737219/200‑1 (MQ=255)                                                                                                                                                     ttGGTGCTGTTGGGGATTCGCAAACGTGATTTGACGGGCGCGCACTCCGCGTGAAATTAGTTTTTGTGGCGCATGGATTTATGCGTATAATGCCGAAGTATTTTTTCACCGAAATTTATGGAACACATTGAATGCAGCTGATTCGAGGCCTGGGAAATTTACCGCAAAATTTGCACGCTTGCGCGTTAACCATCGGTAATTTTGATGGCGTGCATCTGGGGCATCAAGCTATTTTGCGTCATTTGCGGGaa                                                                                      >  1:636804/1‑251 (MQ=255)                                                                                                                                                        gTGCTGTTGGGGATTCGCAAACGTGATTTGACGGGCGCGCACTCCGCGTGAAATTAGTTTTTGTGGCGCATGGATTTATGCGTATAATGCCGAAGTATTTTTTCACCGAAATTTATgg                                                                                                                                                                                                                        <  1:732576/118‑1 (MQ=255)                                                                                                                                                        gTGCTGTTGGGGATTCGCAAACGTGATTTGACGGGCGCGCACTCCGCGTGAAATTAGTTTTTGTGGCGCATGGATTTATGCGTATAATGCCGAAGTATTTTTTCACCGAAATTTATGGAACACATTGAATGCAGCTGATTCGAGGCCTGGGAAATTTACCGCAAAATTTGCACGCTTGCGCGTTAACCATCGGTAATTTTGATGGCGTGCATCTGGGGCATCAAGCTATTTTGCGTCATTTGCGGGaaaaa                                                                                   >  1:172248/1‑251 (MQ=255)                                                                                                                                                          gctgttGGGGATTCGCAAACGTGATTTGACGGGCGCGCACTCCGCGTGAAATTAGTTTTTGTGGCGCATGGATTTATGCGTATAATGCCGAAGTATTTTTTCACCGAAATTTATGGAACACATTGAATGCAGCTGATTCGAGGCCTGGGAAATTTACCGCAAAATTTGCACGCTTGCGCGTTAACCATCGGTAATTTTGATGGCGTGCATCTGGGGCATCAAGCTATTTTGCGTCATTTGCGGGAAAAAg                                                                                  >  1:304790/5‑250 (MQ=255)                                                                                                                                                           ctgttGGGGATTCGCAAACGTGATTTGACGGGCGCGCATTCCGCGTGAAATTTGTTTTTGTGGCGCATGGATTTATGCGTATAATGCCGAAGTATTTTTTCACCGAAATTTATGGAACACATTGAATGCAGCTGATTCGAGGCCTGGGAAAttt                                                                                                                                                                                 >  1:759000/4‑154 (MQ=255)                                                                                                                                                            tgttGGGGATTCGCAAACGTGATTTGACGGGCGCGCACTCCGCGTGAAATTAGTTTTTGTGGCGCATGGATTTATGCGTATAATGCCGAAGTATTTTTTCACCGAAATTTATGGAACACATTGAATGCAGCTGATTCGAGGCCTGGGAAATTTACCGCAAAATTTGCACGCTTGCGCGTTAACCATCGGTAATTTTGATGGCGTGCATCTGGGGCATCAAGCTATTTTGCGTCATTTGCGGGAAAAAGCcg                                                                               >  1:795192/3‑251 (MQ=255)                                                                                                                                                             gttGGGGATTCGCAAACGTGATTTGACGGGCGCGCACTCCGCGTGAAATTAGTTTTTGTGGCGCATGGATTTATGCGTATAATGCCGAAGTATTTTTTCACCGAAATTTATGGAACACATTGAATGCAGCTGATTCGAGGCCTGGGAAATTTACCGCAAAATTTGCACGCTTGCGCGTTAACCATCGGTAATTTTGATGGCGTGCATCTGGGGCATCAAGCTATTTTGCGTCATTTGCGGGAAAAAGCcga                                                                              >  1:618986/2‑251 (MQ=255)                                                                                                                                                             gttGGGGATTCGCAAACGTGATTTGACGGGCGCGCACTCCGCGTGAAATTAGTTTTTGTGGCGCATGGATTTATGCGTATAATGCCGAAGTATTTTTTCACCGAAATTTATGGAACACATTGAATGCAGCTGATTCGAGGCCTGGGAAATTTACCGCAAAATTTGCACGCTTGCGCGTTAACCATCGGTAATTTTGATGGCGTGCATCTGGGGCATCAAGCTATTTTGCGTCATTTGCGGGAAAAAGCcga                                                                              >  1:428132/2‑251 (MQ=255)                                                                                                                                                                    aTTCGCAAACGTGATTTGACGGGCGCGCACTCCGCGTGAAATTAGTTTTTGTGGCGCATGGATTTATGCGTATAATGCCGAAGTATTTTTTCACCGAAATTTATGGAACACATTGAATGCAGCTGATTCGAGGCCTGGGAAATTTACCGCAAAATTTGCACGCTTGCGCGTTAACCATCGGTAATTTTGATGGCGTGCATCTGGGGCATCAAGCTATTTTGCGTCATTTGCGGGAAAAAGCCGACGAATTg                                                                       <  1:146882/251‑1 (MQ=255)                                                                                                                                                                      tCGCAAACGTGATTTGACGGGCGCGCACTCCGCGTGAAATTAGTTTTTGTGGCGCATGGATTTATGCGTATAATGCCGAAGTATTTTTTCACCGAAATTTATGGAACACATTGAATGCAGCTGATTCGAGGCCTGGGAAATTTACCGCAAAATTTGCACGCTTGCGCGTTAAc                                                                                                                                                   <  1:583954/173‑1 (MQ=255)                                                                                                                                                                       cGCAAACGTGATTTGACGGGCGCGCACTCCGCGTGAAATTAGTTTTTGTGGCGCATGGATTTATGCGTATAATGCCGAAGTATTTTTTCACCGAAATTTATGGAACACATTGAATGCAGCTGATTCGAGGCCTGGGAAATTTACCGCAAAATTTGCACGCTTGCGCGTTAACCATCGGTAATTTTGATGGCGTGCATCTGGGGCATCAAGCTATTTTGCGTCATTTGCGGGAAAAAGCCGACGAATTGCAt                                                                    <  1:222100/251‑1 (MQ=255)                                                                                                                                                                         cAAACGTGATTTGACGGGCGCGCACTCCGCGTGAAATTAGTTTTTGTGGCGCATGGATTTATGCGTATAATGCCGAAGTATTTTTTCACCGAAATTTATGGAACACATTGAATGCAGCTGATTCGAGGCCTGGGAAATTTACCGCAAAATTTGCACGCTTGCGCGTTAACCATCGGTAATTTTGATGGCGTGCATCTGGGGCATCAAGCTATTTTGCGTCATTTGCGGGAAAAAGCCGACGAATTGCAtt                                                                   <  1:401648/250‑1 (MQ=255)                                                                                                                                                                             cGTGATTTGGCGGGCGCGCACTCCGCGTGAAATTAGTTTTTGTGGCGCATGGATTTATGCGTATAATGCCGAAGTATTTTTTCACCGAAATTTATGGAACACATTGAATGCAGCTGATTCGAGGCCTGGGAAATTTACCGCAAAATTTGCACGCTTGCGCGTTAACCATCGGTAATTTTGATGGCGTGCATCTGGGGCATCAAGCTATTTTGCGTCATTTGCGGGAAAAAGCCGACGAATTGCATTTGccc                                                              <  1:534564/251‑1 (MQ=255)                                                                                                                                                                             cGTGATTTGACGGGCGCGCACTCCGCGTGAAATTAGTTTTTGTGGCGCATGGATTTATGCGTATAATGCCGAAGTATTTTTTCACCGAAATTTATGGAACACATTGAATGCAGCTGATTCGAGGCCTGGGAAATTTACCGCAAAATTTGCACGCTTGCGCGTTAACCATCGGTAATTTTGATGGCGTGCATCTGGGGCATCAAGCTATTTTGCGTCATTTGCGGGAAAAAGCCGACGAATTGCAt                                                                    >  1:610836/1‑245 (MQ=255)                                                                                                                                                                               tGATTTGACGGGCGCGCACTCCGCGTGAAATTAGTTTTTGTGGCGCATGGATTTATGCGTATAATGCCGAAGTATTTTTTCACCGAAATTTATGGAACACATTGAATGCAGCTGATTCGAGGCCTGGGAAATTTACCGCAAAATTTGCACGCTTGCGCGTTAACCATCGGTAATTTTGATGGCGTGCATCTGGGGCATCAAGCTATTTTGCGTCATTTGCGGGAAAAAGCCGACGAATTGCATTTGCCCAt                                                            >  1:559910/1‑251 (MQ=255)                                                                                                                                                                                gATTTGACGGGCGCGCACTCCGCGTGAAATTAGTTTTTGTGGCGCATGGATTTATGCGTATAATGCCGAAGTATTTTTTCACCGAAATTTATGGAACACAtt                                                                                                                                                                                                                >  1:654370/1‑102 (MQ=255)                                                                                                                                                                                  tttGACGGGCGCGCACTCCGCGTGAAATTAGTTTTTGTGGCGCATGGATTTATGCGTATAATGCCGAAGTATTTTTTCACCGAAATTTATGGAACACATTGAATGCAGCTGATTCGAGGCCTGGGAAATTTACCGCAAAATTTGCACGCTTGCGCGTTAACCATCGGTAATTTTGATGGCGTGCATCTGGGGCATCAAGCTATTTTGCGTCATTTGCGGGAAAAAGCCGACGAATTGCATTTGCCCAtggt                                                         >  1:830881/1‑251 (MQ=255)                                                                                                                                                                                    tGACGGGCGCGCACTCCGCGTGAAATTAGTTTTTGTGGCGCATGGATTTATGCGTATAATGCCGAAGTATTTTTTCACCGAAATTTATGGAACACATTGAATGCAGCTGATTCGAGGCCTGGGAAATTTACCGCAAAATTTGCACGCTTGCGCGTTAACCATCGGTAATTTTGATGGCGTGCATCTGGGGCATCAAGCTATTTTGCGTCATTTGCGGGAAAAAGCCGACGAATTGCATTTGCCCAtggtgg                                                       <  1:845655/251‑1 (MQ=255)                                                                                                                                                                                         ggCGCGCACTCCGCGTGAAATTAGTTTTTGTGGCGCATGGATTTATGCGTATAATGCCGAAGTATTTTTTCACCGAAATTTATGGAACACATTGAATGCAGCTGATTCGAGGCCTGGGaaa                                                                                                                                                                                    <  1:568691/121‑1 (MQ=255)                                                                                                                                                                                         ggCGCGCACTCCGCGTGAAATTAGTTTTTGTGGCGCATGGATTTATGCGTATAATGCCGAAGTATTTTTTCACCGAAATTTATGGAACACATTGAATGCAGCTGATTCGAGGCCTGGGAAATTTACCGCAAAATTTGCACGCTTGCGCGTTAACCATCGGTAATTTTGATGGCGTGCATc                                                                                                                         <  1:662740/180‑1 (MQ=255)                                                                                                                                                                                         ggCGCGCACTCCGCGTGAAATTAGTTTTTGTGGCGCATGGATTTATGCGTATAATGCCGAAGTATTTTTTCACCGAAATTTATGGAACACATTGAATGCAGCTGATTCGAGGCCTGGGAAATTTACCGCAAAATTTGCACGCTTGCGCGTTAACCATCGGTAATTTTGATGGCGTGCATCTGGGGCATCAAGCTATTTTGCGTCATTTGCGGGAAAAAGCCGACGAATTGCAt                                                                    >  1:181985/1‑233 (MQ=255)                                                                                                                                                                                           cgcgcACTCCGCGTGAAATTAGTTTTTGTGGCGCATGGATTTATGCGTATAATGCCGAAGTATTTTTTCACCGAAATTTATGGAACACATTGAATCCAGCTGATTCGAGGCCTGGGAAATTTACCGCAAAATATGCACGCTTGCGCGTTAACCATCGGTAAttt                                                                                                                                       >  1:48915/1‑164 (MQ=255)                                                                                                                                                                                             cgcACTCCGCGTGAAATTAGTTTTTGTGGCGCATGGATTTATGCGTATAATGCCGAAGTATTTTTTCACCGAAATTTATGGAACACATTGAATGCAGCTGATTCGAGGCCTGGGAAATTTACCGCAAAATTTGCACGCTTGCGCGTTAACCATCGGTAATTTTGATGGCGTGCATCTGGGGCATCAATCTATTTTGCGTCATTTGCGGGAAAAAGCCGACGAATTGCATTTGCCCATGGTGGTGATGCTg                                               <  1:139482/250‑1 (MQ=255)                                                                                                                                                                                                   ccGCGTGAAATTAGTTTTTGTGGCGCATGGATTTATGCGTATAATGCCGAAGTATTTTTTCACCGAAATTTATGGAACACATTGAATGCAGCTGATTCGAGGCCTGGGAAATTTACCGCAAAATTTGCACGCTTGCGCGTTAACCATCGGTAATTTTGATGGCGTGCATCTGGGGCATCAAGCTATTTTGCGTCATTTGCGGGAAAAAGCCGACGAATTGCATTTGCCCATGGTGGTGATGCTGTTTGAAc                                        <  1:463009/251‑1 (MQ=255)                                                                                                                                                                                                    cgcgTGAAATTAGTTTTTGTGGCGCATGGATTTATGCGTATAATGCCGAAGTATTTTTTCACCGAAATTTATGGAACACATTGAATGCAGCTGATTCGAGGCCTGGGAAATTTACCGCAAAATTTGCACGCTTGCGCGTTAACCATCGGTAATTTTGATGGCGTGCATCTGGGGCATCAAGCTATTTTGCGTCATTTGCGGGAAAAAGCCGACGAATTGCATTTGCCCATGGTGGTGATGCTGTTTGAAcc                                       <  1:124985/251‑1 (MQ=255)                                                                                                                                                                                                                gTTTTTGTGGCGCATGGATTTATGCGTATAATGCCGAAGTATTTTTTCACCGAAATTTATGGAACACATTGAATGCAGCTGATTCGAGGCCTGGGAAATTTACCGCAAAATTTGCACGCTTGCGCGTTAACCATCGGTAATTTTGATGGCGTGCATCTGGGGCATCAAGCTATTTTGCGTCATTTGCGGGAAAAAGCCGACGAATTGCAtt                                                                   >  1:110477/1‑211 (MQ=255)                                                                                                                                                                                                                                gATTTATGCGTATAATGCCGAAGTATTTTTTCACCGAAATTTATGGAACACATTGAATGCAGCTGATTCGAGGCCTGGGAAATTTACCGCAAAATTTGCACGCTTGCGCGTTAACCATCGGTAATTTTGATGGCGTGCATCTGGGGCATCAAGCTAttt                                                                                                       <  1:260615/159‑1 (MQ=255)                                                                                                                                                                                                                                gATTTATGCGTATAATGCCGAAGTATTTTTTCACCGAAATTTATGGAACACATTGAATGCAGCTGATTCGAGGCCTGGGAAATTTACCGCAAAATTTGCACGCTTGCGCGTTAACCATCGGTAATTTTGATGGCGTGCATCTGGGGCATCAAGCTATTTTGCGTCATTTGCGGGAAAAAGCCGACGAATTGCATTTGCCCATGGTGGTGATGCTGTTTGAACCGCAGCCGCGTGAATATTTTTGCGGTGaa           >  1:571205/1‑251 (MQ=255)                                                                                                                                                                                                                                      tGCGTATAATGCCGAAGTATTTTTTCACCGAAATTTATGGAACACATTGAATGCAGCTGATTCGAGGCCTGGGAAATTTACCGCAAAATTTGCACGCTTGCGCGTTAACCATCGGTAATTTTGATGGCGTGCATCTGGGGCATCAAGCTATTTTGCGTCATTTGCGGGAAAAAGCCGACGAATTGCATTTGCCCATGGTGGTGATGCTGTTTGAACCGCAGCCGCGTGAATATTTTTGCGGTGAAGATgcg     <  1:421263/251‑1 (MQ=255)                                                                                                                                                                                                                                         gTATAATGCCGAAGTATTTTTTCACCGAAATTTATGGAACACATTGAATGCAGCTGATTCGAGGCCTGGGAAATTTACCGCAAAATTTGCACGCTTGCGCGTTAACCATCGGTAATTTTGATGGCGTGCATCTGGGGCATCAAGCTATTTTGCGTCATTTGCGGGAAAAAGCCGACGAATTGCATTTGCCCATGGTGGTGATGCTGTTTGAACCGCAGCCGCGTGAATATTTTTGCGGTGAAg          <  1:351202/243‑1 (MQ=255)                                                                                                                                                                                                                                         gTATAATGCCGAAGTATTTTTTCACCGAAATTTATGGAACACATTGAATGCAGCTGATTCGAGGCCTGGGAAATTTACCGCAAAATTTGCACGCTTGCGCGTTAACCATCGGTAATTTTGATGGCGTGCATCTGGGGCATCAAGCTATTTTGCGTCATTTGCGGGAAAAAGCCGACGAATTGCATTTGCCCATGGTGGTGATGCTGTTTGAACCGCAGCCGCGTGAATATTTTTGCGGTGAAGATGCGCCg  >  1:341128/1‑251 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                    GAAAGTTTTGTTTGCCGCCGTCATCATGGGATTGTTGGTTTGGTACAACAGCCCAAGCCTGACGGAATGGAACGATATGCGCTTTCTCACCCGTGTACATTGGCTGACGTGGCTCATCGTGTTGGCAGCGATGGTTTACGGTGGCATGTTGGTGCTATTGGGGATTCGCAAACGTGATTTGACGGGCGCGCACTCCGCGTGAAATTAGTTTTTGTGGCGCATGGATTTATGCGTATAATACCGAAGTATTTTTTCACCGAAATTTATGGAACACATTGAATGCAGCTGATTCGAGGCCTGGGAAATTTACCGCAAAATTTGCACGCTTGCGCGTTAACCATCGGTAATTTTGATGGCGTGCATCTGGGGCATCAAGCTATTTTGCGTCATTTGCGGGAAAAAGCCGACGAATTGCATTTGCCCATGGTGGTGATGCTGTTTGAACCGCAGCCGCGTGAATATTTTTGCGGTGAAGATGCGCCG  >  1/1908227‑1908709

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑