Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,901,122	T→C	intergenic (‑1379/–)	unknown ← / –	tRNA‑Met‑CAT/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,901,122	0	T	C	100.0%	197.0 / NA	67	intergenic (‑1379/–)	unknown/–	tRNA‑Met‑CAT/–
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (45/22);  total (45/22)

GTGTGATTCCATTGGGTAGCGTGTTGCTGGTGGAGGTGCCGCAAATAGATAATGACGGCAACTGGACAGGGCAACATCAGTTACACCTCATGGTTGCGCTGGATGTGGGCGGTGCCGTGAACGGACACCATTTCGATTTATATCGCGGTATCGGCGATCAGGCGGGACATATTGCCGGCTTGTCGAAACATTACGGTCGCGTTTGGGTGTTACGTTAATGGCG‑‑CACATTGACAACTATGAACAACGCTTTGGCGGTATCGGCCGTTTATATGGCGCCGATGCTTTAGCGCGCTTGCGTAAAACGCACATTTGCGTAATCGGTATCGGCGGCGTCGGTTCCTGGGCGGTGGAAGCTCTCGCCCGTTCCGGCATCGGCAAGATTACGATGATTGATATGGATGATATTTGTGTTACCAATATTAATCGCCAGATTCATGCCTTGAGCGGTAATATCGGGCAACTCAAAACCGACATTA  >  1/1900885‑1901360                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                               gtgtGATTCCATTGGGTAGCGTGTTGCTGGTGGAGGTGCCGCAAATAGATAATGACGGCAACTGGACAGGGCAACATCAGTTACACCTCATGGTTGCGCTGGATGTGGGCGGTGCCGTGAACGGACACCATTTCGATTTATATCGCGGTATCGGCGATCAGGCGGGACATATTGCCGGCTTGTCGAAACATTACGGTCGCGTTTGGGTGTTACGTTAATGGCG‑‑CACATTGACAACTACGAACAACGCTTTg                                                                                                                                                                                                                                   >  1:485608/1‑251 (MQ=255)   gtgATTCCATTGGGTAGCGTGTTGCTGGTGGAGGTGCCGCAAATAGATAATGACGGCAACTGGACAGGGCAACATCAGTTACACCTCATGGTTGCGCTGGATGTGGGCGGTGCCGTGAACGGACACCATTTCGATTTATATCGCGGTATCGGCGATCAGGCGGGACATATTGCCGGCTTGTCGAAACATTACGGTCGCGTTTGGGTGTTACGTTAATGGCG‑‑CACATTGACAACTACGAACAACGCTTTgg                                                                                                                                                                                                                                  >  1:669395/1‑250 (MQ=255)    tgATTCCATTGGGTAGCGTGTTGCTGGTGGAGGTGCCGCAAATAGATAATGACGGCAACTGGACAGGGCAACATCAGTTACACCTCATGGTTGCGCTGGATGTGGGCGGTGCCGTGAACGGACACCATTTCGATTTATATCGCGGTATCGGCGATCAGGCGGGACATATTGCCGGCTTGTCGAAACATTACGGTCGCGTTTGGGTGTTACGTTAATGGCG‑‑CACATTGACAGCTACGAACAACGCTTTGGc                                                                                                                                                                                                                                 <  1:765324/250‑1 (MQ=255)    tgATTCCATTGGGTAGCGTGTTGCTGGTGGAGGTGCCGCAAATAGATAATGACGGCAACTGGACAGGGCAACATCAGTTACACCTCATGGTTGCGCTGGATGTGGGCGGTGCCGTGAACGGACACCATTTCGATTTATATCGCGGTATCGGCGATCAGGCGGGACATATTGCCGGCTTGTCGAAACATTACGGTCGCGTTTGGGTGTTACGTTAATGGCG‑‑CACATTGACAACTACGAACAACGCTTTGGc                                                                                                                                                                                                                                 <  1:81150/250‑1 (MQ=255)      aTTCCATTGGGTAGCGTGTTGCTGGTGGAGGTGCCGCAAATAGATAATGACGGCAACTGGACAGGGCAACATCAGTTACACCTCATGGTTGCGCTGGATGTGGGCGGTGCCGTGAACGGACACCATTTCGATTTATATCGCGGTATCGGCGATCAGGCGGGACATATTGCCGGCTTGTCGAAACATTACGGTCGCGTTTGGGTGTTACGTTAATGGCG‑‑CACATTGACAACTACGAACAACGCTTTGGCGGt                                                                                                                                                                                                                              >  1:495909/1‑251 (MQ=255)         ccATTGGGTAGCGTGTTGCTGGTGGAGGTGCCGCAAATAGATAATGACGGCAACTGGACAGGGCAACATCAGTTACACCTCATGGTTGCGCTGGATGTGGGCGGTGCCGTGAACGGACACAATTTCGATTTATATCGCGGTATCGGCGATCAGGCGGGACATATTGCCGGCTTGTCGAAACATTACGGTCGCGTTTGGGTGTTACGTTAATGGCG‑‑CACATTGACAACTACGAACAACGCTTTGGCGGTAt                                                                                                                                                                                                                            >  1:355158/1‑250 (MQ=255)              gggTAGCGTGTTGCTGGTGGAGGTGCCGCAAATAGATAATGACGGCAACTGGACAGGGCAACATCAGTTACACCTCATGGTTGCGCTGGATGTGGGCGGTGCCGTGAACGGACACCATTTCGATTTATATCGCGGTATCGGCGATCAGGCGGGACATATTGCCGGCTTGTCGAAACATTACGGTCGCGTTTGGGTGTTACGTTAATGGCG‑‑CACATTGACAACTACGaac                                                                                                                                                                                                                                            >  1:857197/1‑229 (MQ=255)              gggTAGCGTGTTGCTGGTGGAGGTGCCGCAAATAGATAATGACGGCAACTGGACAGGGCAACATCAGTTACACCTCATGGTTGCGCTGGATGTGGGCGGTGCCGTGAACGGACACCATTTCGATTTATATCGCGGTATCGGCGATCAGGCGGGACATATTGCCGGCTTGTCGAAACATTACGGTCGCGTTTGGGTGTTACGTTAATGGCG‑‑CACATTGACAACTACGAACAACGCTTTGGCGGTATCGGCCg                                                                                                                                                                                                                      >  1:535956/1‑251 (MQ=255)                   gCGTGTTGCTGGTGGAGGTGCCGCAAATAGATAATGACGGCAACTGGACAGGGCAACATCAGTTACACCTCATGGTTGCGCTGGATGTGGGCGGTGCCGTGAACGGACACCATTTCGATTTATATCGCGGTATCGGCGATCAGGCGGGACATATTGCCGGCTTGTCGAAACATTACGGTCGCGTTTGGGTGTTACGTTAATGGCG‑‑CACATTGACAACTACGAACAACGCTTTGGCGGTATCGGcc                                                                                                                                                                                                                       <  1:354841/245‑1 (MQ=255)                   gCGTGTTGCTGGTGGAGGTGCCGCAAATAGATAATGACGGCAACTGGACAGGGCAACATCAGTTACACCTCATGGTTGCGCTGGATGTGGGCGGTGCCGTGAACGGACACCATTTCGATTTATATCGCGGTATCGGCGATCAGGCGGGACATATTGCCGGCTTGTCGAAACATTACGGTCGCGTTTGGGTGTTACGTTAATGGCG‑‑CACATTGACAACTACGAACAACGCTTTGGCGGTATCGGCCGTTtat                                                                                                                                                                                                                 >  1:694671/1‑251 (MQ=255)                       gtTGCTGGTGGAGGTGCCGCAAATAGATAATGACGGCAACTGGACAGGGCAACATCAGTTACACCTCATGGTTGCGCTGGATGTGGGCGGTGCCGTGAACGGACACCATTTCGATTTATATCGCGGTATCGGCGATCAGGCGGGACATATTGCCGGCTTGTCGAAACATTACGGTCGCGTTTGGGTGTTACGTTAATGGCG‑‑CACATTGACAACTACGaac                                                                                                                                                                                                                                            >  1:118297/1‑220 (MQ=255)                        ttGCTGGTGGAGGTGCCGCAAATAGATAATGACGGCAACTGGACAGGGCAACATCAGTTACACCTCATGGTTGCGCTGGATGTGGGCGGTGCCGTGAACGGACACCATTTCGATTTATATCGCGGTATCGGCGATCAGGCGGGACATATTGCCGGCTTGTCGAAACATTACGGTCGCGTTTGGGTGTTACGTTAATGGCG‑‑CACATTGACAACTACGAACAACGCTTTGGCGGTATCGGCCGTTTATATgg                                                                                                                                                                                                             <  1:236367/250‑1 (MQ=255)                           cTGGTGGAGGTGCCGCAAATAGATAATGACGGCAACTGGACAGGGCAACATCAGTTACACCTCATGGTTGCGCTGGATGTGGGCGGTGCCGTGAACGGACACCATTTCGATTTATATCGCGGTATCGGCGATCAGGCGGGACATATTGCCGGCTTGTCGAAACATTACGGTCGCGATTGGGTGTTACGTTAATGGCG‑‑CACATTGACAACTACGAACAACGCTTTCGCGGTATCGGCCGTTTATATGgcgc                                                                                                                                                                                                          >  1:363656/1‑250 (MQ=255)                            tggtggAGGTGCCGCAAATAGATAATGACGGCAACTGGACAGGGCAACATCAGTTACACCTCATGGTTGCGCTGGATGTGGGCGGTGCCGTGAACGGACACCATTTCGATTTATATCGCGGTATCGGCGATCAGGCGGGACATATTGCCGGCTTGTCGAAACATTACGGTCGCGTTTGGGTGTTACGTTAATGGCG‑‑CACATTGACAACTACGAACAACGCTTTGGCGGTATCGGCCGTTTATATGGCGCCg                                                                                                                                                                                                        >  1:411453/1‑251 (MQ=255)                                      gCCGCAAATAGATAATGACGGCAACTGGACAGGGCAACATCAGTTACACCTCATGGTTGCGCTGGATGTGGGCGGTGCCGTGAACGGACACCATTTCGATTTATATCGCGGTATCGGCGATCAGGCGGGACATATTGCCGGCTTGTCGAAACATTACGGTCGCGTTTGGGTGTTACGTTAATGGCG‑‑CACATTGACAACTACGAACAACGCTTTGGCGGTATCGGCCGTTTATATGgcgc                                                                                                                                                                                                          >  1:363533/1‑239 (MQ=255)                                      gCCGCAAATAGATAATGACGGCAACTGGACAGGGCAACATCAGTTACACCTCATGGTTGCGCTGGATGTGGGCGGTGCCGTGAACGGACACCATTTCGATTTATATCGCGGTATCGGCGATCAGGCGGGACATATTGCCGGCTTGTCGAAACATTACGGTCGCGTTTGGGTGTTACGTTAATGGCG‑‑CACATTGACAACTACGAACAACGCTTTGGCGGTATCGGCCGTTTATATGGCGCCGATGCTTTAg                                                                                                                                                                                               >  1:582923/1‑250 (MQ=255)                                       ccGCAAATAGATAATGACGGCAACTGGACAGGGCAACATCAGTTACACCTCATGGTTGCGCTGGATGTGGGCGGTGCCGTGAACGGACACCATTTCGATTTATATCGCGGTATCGGCGATCAGGCGGGACATATTGCCGGCTTGTCGAAACATTACGGTCGCGTTTGGGTGTTACGTTAATGGCG‑‑CACATTGACAACTACGAACAACGCTTTGGCGGTATCGGCCGTTTATATGGCGCCGATGCTTTAg                                                                                                                                                                                               >  1:809937/1‑249 (MQ=255)                                                gATAATGACGGCAACTGGACAGGGCAACATCAGTTACACCTCATGGTTGCGCTGGATGTGGGCGGTGCCGTGAACGGACACCATTTCGATTTATATCGCGGTATCGGCGATCAGGCGGGACATATTGCCGGCTTGTCGAAACATTACGGTCGCGTTTGGGTGTTACGTTAATGGCG‑‑CACATTGACAACTACGAACAACGCTTTGGCGGTATCGGCCGTTTATATGGCGCCGATGCTTTAGCGCGCTTGCGt                                                                                                                                                                                    >  1:841789/1‑251 (MQ=255)                                                  tAATGACGGCAACTGGACAGGGCAACATCAGTTACACCTCATGGTTGCGCTGGATGTGGGCGGTGCCGTGAACGGACACCATTTCGATTTATATCGCGGTATCGGCGATCAGGCGGGACATATTGCCGGCTTGTCGAAACATTACGGTCGCGTTTGGGTGTTACGTTAATGGCG‑‑CACATTGACAACTACGAACAACGCTTTGGCGGTATCGGCCGTTTATATGGCGCCGATGCTTTAGCGCGCTTGCGTaa                                                                                                                                                                                  <  1:302996/251‑1 (MQ=255)                                                           cAACTGGACAGGGCAACATCAGTTACACCTCATGGTTGCGCTGGATGTGGGCGGTGCCGTGAACGGACACCATTTCGATTTATATCGCGGTATCGGCGATCAGGCGGGACATATTGCCGGCTTGTCGAAACATTACGGTCGCGTTTGGGTGTTACGTTAATGGCG‑‑CACATTGACAACTACGAACAACGCTTTGGCGGTATCGGCCGTTTATATGGCGCCGATGCTTTAGCGCGCTTGCGTAAAACGCACAt                                                                                                                                                                         >  1:723554/1‑251 (MQ=255)                                                           cAACTGGACAGGGCAACATCAGTTACACCTCATGGTTGCGCTGGATGTGGGCGGTGCCGTGAACGGACACCATTTCGATTTATATCGCGGTATCGGCGATCAGGCGGGACATATTGCCGGCTTGTCGAAACATTACGGTCGCGTTTGGGTGTTACGTTAATGGCG‑‑CACATTGACAACTACGAACAACGCTTTGGCGGTATCGGCCGTTTATATGGCGCCGATGCTTTAGCGCGCTTGCGTAAAACGCACAt                                                                                                                                                                         >  1:81036/1‑251 (MQ=255)                                                                              cAGTTACACCTCATGGTTGCGCTGGATGTGGGCGGTGCCGTGAACGGACACCATTTCGATTTATATCGCGGTATCGGCGATCAGGCGGGACATATTGCCGGCTTGTCGAAACATTACGGTCGCGTTTGGGTGTTACGTTAATGGCG‑‑CACATTGACAACTACGAACAACGCTTTGGCGGTATc                                                                                                                                                                                                                           >  1:633441/1‑182 (MQ=255)                                                                              cAGTTACACCTCATGGTTGCGCTGGATGTGGGCGGTGCCGTGAACGGACACCATTTCGATTTATATCGCGGTATCGGCGATCAGGCGGGACATATTGCCGGCTTGTCGAAACATTACGGTCGCGTTTGGGTGTTACGTTAATGGCG‑‑CACATTGACAACTACGAACAACGCTTTGGCGGTATCGGCCGTTTATATg                                                                                                                                                                                                              <  1:567495/195‑1 (MQ=255)                                                                              cAGTTACACCTCATGGTTGCGCTGGATGTGGGCGGTGCCGTGAACGGACACCATTTCGATTTATATCGCGGTATCGGCGATCAGGCGGGACATATTGCCGGCTTGTCGAAACATTACGGTCGCGTTTGGGTGTTACGTTAATGGCG‑‑CACATTGACAACTACGAACAACGCTTTGGCGGTATCGGCCGTTTATATGGCGCCGATGCTTTAGCGCGCTTGCGTAAAAc                                                                                                                                                                               <  1:168421/226‑1 (MQ=255)                                                                              cAGTTACACCTCATGGTTGCGCTGGATGTGGGCGGTGCCGTGAACGGACACCATTTCGATTTATATCGCGGTATCGGCGATCAGGCGGGACATATTGCCGGCTTGTCGAAACATTACGGTCGCGTTTGGGTGTTACGTTAATGGCG‑‑CACATTGACAACTACGAACAACGCTTTGGCGGTATCGGCCGTTTATATGGCGCCGATGCTTTAGCGCGCTTGCGTAAAACGCACATTTGCGTAATCGGTATcgg                                                                                                                                                       >  1:484245/1‑250 (MQ=255)                                                                                      ccTCATGGTTGCGCTGGATGTGGGCGGTGCCGTGAACGGACACCATTTCGATTTATATCGCGGTATCGGCGATCAGGCGGGACATATTGCCGGCTTGTCGAAACATTACGGTCGCGTTTGGGTGTTACGTTAATGGCG‑‑CACATTGACAACTACGAACAACGCTTTGGCGGTATCGGCCGTTTATATGGCGCCGATGCTTTAg                                                                                                                                                                                               <  1:737663/202‑1 (MQ=255)                                                                                      ccTCATGGTTGCGCTGGATGTGGGCGGTGCCGTGAACGGACACCATTTCGATTTATATCGCGGTATCGGCGATCAGGCGGGACATATTGCCGGCTTGTCGAAACATTACGGTCGCGTTTGGGTGTTACGTTAATGGCG‑‑CACATTGACAACTACGAACAACGCTTTGGCGGTATCGGCCGTTTATATGGCGCCGATGCTTTAGCGCGCTTGCGTaa                                                                                                                                                                                  >  1:722594/1‑215 (MQ=255)                                                                                           tGGTTGCGCTGGATGTGGGCGGTGCCGTGAACGGACACCATTTCGATTTATATCGCGGTATCGGCGATCAGGCGGGACATATTGCCGGCTTGTCGAAACATTACGGTCGCGTTTGGGTGTTACGTTAATGGCG‑‑CACATTGACAACTACGAACAACGCTTTGGCGGTATCGGCCGTTTATATGGCGCCGATGCTTTAGCGCGCTTGCGTAAAACGCACATTTGCGTAATCGGTATCGGCGGCGTCGGTTc                                                                                                                                           >  1:522063/1‑249 (MQ=255)                                                                                                 cgcTGGATGTGGGCGGTGCCGTGAACGGACACCATTTCGATTTATATCGCGGTATCGGCGATTAGGCGGGACATATTGCCGGCTTGTCGAAACATTACGGTCGCGTTTGGGTGTTACGTTAATGGCG‑‑CACATTGACAACTACGAACAACGCTTTGGCGGTATCGGCCGTTTATATGGCGCCGATGCTTTAGCGCGCTTGCGTAAAACGCACATTTGCGTAATCGGTATcggc                                                                                                                                                      <  1:423458/232‑1 (MQ=255)                                                                                                     ggATGTGGGCGGTGCCGTGAACGGACACCATTTCGATTTATATCGCGGTATCGGCGATCAGGCGGGACATATTGCCGGCTTGTCGAAACATTACGGTCGCGTTTGGGTGTTACGTTAATGGCG‑‑CACATTGACAACTACGAACAACGCTTTGGCGGTATCGGCCGTTTATATGGCGCCGATGCTTTAgc                                                                                                                                                                                              >  1:759921/1‑188 (MQ=255)                                                                                                     ggATGTGGGCGGTGCCGTGAACGGACACCATTTCGATTTATATCGCGGTATCGGCGATCAGGCGGGACATATTGCCGGCTTGTCGAAACATTACGGTCGCGTTTGGGTGTTACGTTAATGGCG‑‑CACATTGACAACTACGAACAACGCTTTGGCGGTATCGGCCGTTTATATGGCGCCGATGCTTTAGCGCGCTTGCGTAAAACGCACATTTGCGTAATc                                                                                                                                                               <  1:327513/219‑1 (MQ=255)                                                                                                     ggATGTGGGCGGTGCCGTGAACGGACACCATTTCGATTTATATCGCGGTATCGGCGATCAGGCGGGACATATTGCCGGCTTGTCGAAACATTACGGTCGCGTTTGGGTGTTACGTTAATGGCG‑‑CACATTGACAACTACGAACAACGCTTTGGCGGTATCGGCCGTTTATATGGCGCCGATGCTTTAGCGCGCTTGCGTAAAACGCACATTTGCGTAATCGGTATCGGCGGCGTCGGTTCCTggg                                                                                                                                      >  1:215488/1‑244 (MQ=255)                                                                                                     ggATGTGGGCGGTGCCGTGAACGGACACCATTTCGATTTATATCGCGGTATCGGCGATCAGGCGGGACATATTGCCGGCTTGTCGAAACATTACGGTCGCGTTTGGGTGGTACGTTAATGGCG‑‑CACATTGACAACTACGAACAACGCTTTGGCGGTATCGGCCGTTTATATTGCGCCGATGCTTTAGCGCGCTTGCGTAAAACGCACATTTGCGTAATCGGTATCGGCTGCGTCCGTTTCTGGGCGGTggg                                                                                                                               >  1:483236/1‑250 (MQ=255)                                                                                                                             acacCATTTCGATTTATATCGCGGTATCGGCGATCAGGCGGGACATATTGCCGGCTTGTCGAAACATTACGGTCGCGTTTGGGTGTTACGTTAATGGCG‑‑CACATTGACAACTACGAACAACGCTTTGGCGGTATCGGCCGTTTATATGGCGCCGATGCTTTAGCGCGCTTGCGTAAAACGCACATTTGCGTAATCGGTATCGGCGGCGTCGGTTCCTGGGCGGGGGAAGCTCTCGCCCGTTCCGGCACCgg                                                                                                       >  1:140812/1‑251 (MQ=255)                                                                                                                                ccATTTCGATTTATATCGCGGTATCGGCGATCAGGCGGGACATATTGCCGGCTTGTCGAAACATTACGGTCGCGTTTGGGTGTTACGTTAATGGCG‑‑CACATTGACAACTACGAACAACGCTTTGGCGGTATCGGCCGTTTatat                                                                                                                                                                                                               >  1:138637/1‑144 (MQ=255)                                                                                                                                   tttCGATTTATATCGCGGTATCGGCGATCAGGCGGGACATATTGCCGGCTTGTCGAAACATTACGGTCGCGTTTGGGTGTTACGTTAATGGCG‑‑CACATTGACAACTACGAACAACGCTTTGGCGGTATCGGCCGTTTATATGGCGCCGATg                                                                                                                                                                                                     >  1:764565/1‑151 (MQ=255)                                                                                                                                       gATTTATATCGCGGTATCGGCGATCAGGCGGGACATATTGCCGGCTTGTCGAAACATTACGGTCGCGTTTGGGTGTTACGTTAATGGCG‑‑CACATTGACAACTACGAACAACGCTTTGGCGGTATCGGCCGTTTATATGGCGCCGATGCTTTAGCGCGCTTGCGTaaaa                                                                                                                                                                                >  1:715381/1‑168 (MQ=255)                                                                                                                                        aTTTATATCGCGGTATCGGCGATCAGGCGGGACATATTGCCGGCTTGTCGAAACATTACGGTCGCGTTTGGGTGTTACGTTAATGGCG‑‑CACATTGACAACTACGAACAACGCTTTGGCGGTATCGGCCGTTTATATGGCGCCGATGCTTTAGCGCGCTTGCGTAAAACGCACATTTGCGTAATCGGTATCGGCGGCGTCGGTTCCTGGGCGGTGGAAGCTCTCGCCCGTTCCGGCATCGGCAAGATTACga                                                                                            <  1:575919/251‑1 (MQ=255)                                                                                                                                            atatCGCGGTATCGGCGATCAGGCGGGACATATTGCCGGCTTGTCGAAACATTACGGTCGCGTTTGGGTGTTACGTTAATGGCGCACACATTGACAACTACGAACAACGCTTTGGCGGTATCGGCCGTTTATATGGCGCCGATGCTTTAGCGCGCTTGCGTaaaa                                                                                                                                                                                <  1:156185/165‑1 (MQ=255)                                                                                                                                            atatCGCGGTATCGGCGATCAGGCGGGACATATTGCCGGCTTGTCGAAACATTACGGTCGCGTTTGGGTGTTACGTTAATGGCG‑‑CACATTGACAACTACGAACAACGCTTTGGCGGTATCGGCCGTTTATATGGCGCCGATg                                                                                                                                                                                                     <  1:631762/142‑1 (MQ=255)                                                                                                                                            atatCGCGGTATCGGCGATCAGGCGGGACATATTGCCGGCTTGTCGAAACATTACGGTCGCGTTTGGGTGTTACGTTAATGGCG‑‑CACATTGACAACTACGAACAACGCTTTGGCGGTATCGGCCGTTTATATGGCGCCGATGCTTTAGCGCGCTTGCGTAAAACGCACATTTGCGTAATCGGTATCGGCGGCGTCGGTTCCTGGGCGGTGGAAGCTCTCGCCCGTTCCGGCATCGGCAAGATTACgatgat                                                                                        >  1:111294/1‑251 (MQ=255)                                                                                                                                            atatCGCGGTATCGGCGATCAGGCGGGACATATTGCCGGCTTGTCGAAACATTACGGTCGCGTTTGGGTGTTACGTTAATGGCG‑‑CACATTGACAACTACGAACAACGCTTTGGCGGTATCGGCCGTTTATATGGCGCCGATGCTTTAGCGCGCTTGCGTAAAACGCACATTTGCGTAATCGGTATCGGCGGCGTCGGTTCCTGGGCGGTGGAAGCTCTCGCCCGTTCCGGCATCGGCAAGATTACgatgat                                                                                        >  1:757516/1‑251 (MQ=255)                                                                                                                                            atatCGCGGTATCGGCGATCAGGCGGGACATATTGCCGGCTTGTCGAAACATTACGGTCGCGTTTGGGTGTTACGTTAATGGCG‑‑CACATTGACAACTACGAACAACGCTTTGGCGGTATCGGCCGTTTATATGGCGCCGATGCTTTAGCGCGCTTGCGTAAAACGCACATTTGCGTAATCGGTATCGGCGGCGTCGGTTCCTGGGCGGTGGAAGCTCTCGCCCGTTCCGGCATCGGCAAGATTACgatgat                                                                                        >  1:523018/1‑251 (MQ=255)                                                                                                                                               tCGCGGTATCGGCGATCAGGCGGGACATATTGCCGGCTTGTCGAAACATTACGGTCGCGTTTGGGTGTTACGTTAATGGCG‑‑CACATTGACAACTACGAACAACGCTTTGGCGGTATCGGCCGTTTATATGGCGCCGATGCTTTAGCGCGCTTGCGTAAAACGCACATTTGCGTAATCGGTATCGGCGGCGTCGGTTCCTGGGCGGTGGAAGCTCTCGCCCGTTCCGGCATCGGCAAGATTACGAtgattga                                                                                     >  1:570818/1‑251 (MQ=255)                                                                                                                                               tCGCGGTATCGGCGATCAGGCGGGACATATTGCCGGCTTGTCGAAACATTACGGTCGCGTTTGGGTGTTACGTTAATGGCG‑‑CACATTGACAACTACGAACAACGCTTTGGCGGTATCGGCCGTTTATATGGCGCCGATGCTTTAGCGCGCTTGCGTAAAACGCACATTTGCGTAATCGGTATCGGCGGCGTCGGTTCCTGGGCGGTGGAAGCTCTCGCCCGTTCCGGCATCGGCAAGATTACGAtgattga                                                                                     >  1:240826/1‑251 (MQ=255)                                                                                                                                                    gTATCGGCGATCAGGCGGGACATATTGCCGGCTTGTCGAAACATTACGGTCGCGTTTGGGTGTTACGTTAATGGCG‑‑CACATTGACAACTACGAACAACGCTTTGGCGGTATCGGCCGTTTatat                                                                                                                                                                                                               >  1:322660/1‑124 (MQ=255)                                                                                                                                                    gTATCGGCGATCAGGCGGGACATATTGCCGGCTTGTCGAAACATTACGGTCGCGTTTGGGTGTTACGTTAATGGCG‑‑CACATTGACAACTACGAACAACGCTTTGGCGGTATCGGCCGTTTATATGGCGCCGATGCTTTAGCGCGCTTGCGTAAAACGCACATTTGCGTAATCGGTATCGGCGGCGTCGGTTCCTGGGCGGTGGAAGCTCTCGCCCGTTCCGGCATCGGCAAGATTACGATGATTGatat                                                                                  <  1:200848/249‑1 (MQ=255)                                                                                                                                                    gTATCGGCGATCAGGCGGGACATATTGCCGGCTTGTCGAAACATTACGGTCGCGTTTGGGTGTTACGTTAATGGCG‑‑CACATTGACAACTACGAACAACGCTTTGGCGGTATCGGCCGTTTATATGGCGCCGATGCTTTAGCGCGCTTGCGTAAAACGCACATTTGCGTAATCGGTATCGGCGGCGTCGGTTCCTGGGCGGTGGAAGCTCTCGCCCGTTCCGGCATCGGCAAGATTACGATGATTGATATgg                                                                                >  1:721460/1‑251 (MQ=255)                                                                                                                                                             aTCAGGCGGGACATATTGCCGGCTTGTCGAAACATTACGGTCGCGTTTGGGTGTTACGTTAATGGCG‑‑CACATTGACAACTACGAACAACGCTTTGGCGGTATCGGCCGTTTATATGGCGCCGATGCTTTAGCGCGCTTGCGTAAAACGCACATTTGCGTAATCGGTATCGGCGGCGTCGGTTCCTGGGCGGTGGAAGCTCTCGCCCGTTCCGGCATCGACAAGATTACGATGATTGATATGGATGATAttt                                                                       >  1:842564/1‑251 (MQ=255)                                                                                                                                                              tCAGGCGGGACATATTGCCGGCTTGTCGAAACATTACGGTCGCGTTTGGGTGTTACGTTAATGGCG‑‑CACATTGACAACTACGAACAACGCTTTGGCGGTATCGGCCGTTTATATGGCGCCGATGCTTTAGCGCGCTTGCGTAAAACGCACATTTGCGTAATCGGTATCGGCGGCGTCGGTTCCTGGGCGGTGGAAGCTCTCGCCCGTTCCGGCATCGGCAAGATTACGATGATTGATATGGATGATATTtg                                                                      <  1:825739/251‑1 (MQ=255)                                                                                                                                                                     ggACATATTGCCGGCTTGTCGAAACATTACGGTCGCGTTTGGGTGTTACGTTAATGGCG‑‑CACATTGACAACTACGAACAACGCTTTGGCGGTATCGGCCGTTTATATGGCGCCGATGCTTTAGCGCGCTTGCGTAAAACGCACATTTGCGTAATCGGTATCGGCGGCGTCGGTTCCTGGGCGGTGGAAGCTCTCGCCCGTTCCGGCATCGGCAAGATTACGATGATTGATATGGATGATATTtgtg                                                                    >  1:773309/1‑246 (MQ=255)                                                                                                                                                                           atTGCCGGCTTGTCGAAACATTACGGTCGCGTTTGGGTGTTACGTTAATGGCG‑‑CACATTGACAACTACGAACAACGCTTTGGCGGTATCGGCCGTTTATATGGCGCCGATg                                                                                                                                                                                                     <  1:585340/111‑1 (MQ=255)                                                                                                                                                                                cGGCTTGTCGAAACATTACGGTCGCGTTTGGGTGTTACGTTAATGGCG‑‑CACATTGACAACTACGAACAACGCTTTGGCGGTATCGGCCGTTTATATGGCGCCGATGCTTTAGCGCGCTTGCGTAAAACGCACATTTGCGTAATCGGTATCGGCGGCGTCGGTTCCTGGGCGGTGGAAGCTCTCGCCCGTTCCGGCATCGGCAAGATTACGATGATTGATATGGATGATATTTGTGTTACCAATATTAATCg                                                    <  1:625002/251‑1 (MQ=255)                                                                                                                                                                                             cATTACGGTCGCGTTTGGGTGTTACGTTCATGGCG‑‑CACATTGACAACTACGAACAACGCTTTGGCGGTATCGGCCGTTTATATGGCGCCGATGCTTTAGCGCGCTTGCGTAAAACGCACATTTGCGTAATCGGTATCGGCGGCGTTGGCTCCCTGGCGGTCGAAGCTCTCGCCCGTTCCCGCATCGGCAAGTGTAAGATGACTGATATGGATGATATTTCTGTTACCAATATTAATCGCCAAACTCATGc                                        >  1:247748/1‑250 (MQ=255)                                                                                                                                                                                             cATTACGGTCGCGTTTGGGTGTTACGTTAATGGCG‑‑CACATTGACAACTACGAACAACGCTTTGGCGGTATCGGCCGTTTATATGGCGCCGATGCTTTAGCGCGCTTGCGTTAAACGCACATTTGCGTAATCGGTATCGGCGGCGTCGGTTCCTGGGCGGTGGAAGCTCTCGCCCGTTCCGGCATCGGCAAGATTACGATGATTGATATGGATGATATTtgtg                                                                    >  1:730848/1‑222 (MQ=255)                                                                                                                                                                                                        cgTTTGGGTGTTACGTTAATGGCG‑‑CACATTGACAACTACGAACAACGCTTTGGCGGTATCGGCCGTTTATATGGCGCCGATGCTTTAGCGCGCTTGCGTAAAACGCACATTTGCGTAATCGGTATCGGCGGCGTCGGTTCCTGGGCGGTGGAAGCTCTCGCCCGTTCCGGCATCGGCAAGAt                                                                                                 >  1:51899/1‑182 (MQ=255)                                                                                                                                                                                                        cgTTTGGGTGTTACGTTAATGGCG‑‑CACATTGACAACTACGAACAACGCTTTGGCGGTATCGGCCGTTTATATGGCGCCGATGCTTTAGCGCGCTTGCGTAAAACGCACATTTGCGTAATCGGTATCGGCGGCGTCGGTTCCTGGGCGGTGGAAGCTCTCGCCCGTTCCGGCATCGGCAAGATTACGATGATTGATATGGATGATATTTGTGTTACCAATATTAATCGCCAGATTCATGCCTTGAGCGGTaa                            <  1:420199/251‑1 (MQ=255)                                                                                                                                                                                                              ggTGTTACGTTAATGGCG‑‑CACATTGACAACTACGAACAACGCTTTGGCGGTATCGGCCGTTTATATGGCGCCGATGCTTTAGCGCGCTTGCGTAAAACGCACATTTGCGTAATCGGTATCGGCGGCGTCGGTTCCTGGGCGGTGGAAGCTCTCGCCCGTTCCGGCATCGGCAAGATTACGATGATTGATATGGATGATATTTGTGTTACCAATATTAATCGCCAGATTCATGCCt                                      <  1:667833/235‑1 (MQ=255)                                                                                                                                                                                                               gtgtTACGTTAATGGCG‑‑CACATTGACAACTACGAACAACGCTTTGGCGGTATCGGCCGTTTATATg                                                                                                                                                                                                              <  1:658014/66‑1 (MQ=255)                                                                                                                                                                                                               gtgtTACGTTAATGGCG‑‑CACATTGACAACTACGAACAACGCTTTGGCGGTATCGGCCGTTTATATGGCGCCGATGCTTTAGCGCGCTTGCGTAAAACGCACAtt                                                                                                                                                                        <  1:635745/104‑1 (MQ=255)                                                                                                                                                                                                               gtgtTACGTTAATGGCG‑‑CACATTGACAACTACGAACAACGCTTTGGCGGTATCGGCCGTTTATATGGCGCCGATGCTTTAGCGCGCTTGCGTAAAACGCACATTTGCGTAATCGGTATCGGCGGCGTCGGTTCCTGGGCGGTGGAAGCTCTCGCCCGTTCCCGCATTGGCAAGATTACGATGATTGATATGGATGATATTTGTGTTACCAATATTAATCGCCAGATTCATGCCTTGAGCGGTAATATCggg                     >  1:570135/1‑251 (MQ=255)                                                                                                                                                                                                                     cGTTAATGGCG‑‑CACATTGACAACTACGAACAACGCTTTGGCGGTATCGGCCGTTTATATGGCGCCGATGCTTTAGCGCGCTTGCGTAAAACGCACATTTGCGTAATCGGTATCGGCGGCGTCGGTTCCTGGGCGGTGGAAGCTCTCGCCCGTTCCGGCATCGGCAAGATTACGATGATTGATATGGATGATATTTGTGTTACCAATATTAATCGCCAGATTCATGCCTTGAGCGGTAATATc                        <  1:510310/242‑1 (MQ=255)                                                                                                                                                                                                                        tAATGGCG‑‑CACATTGACAACTACGAACAACGCTTTGGCGGTATCGGCCGTTTATATGGCGCCGATGCTTTAGCGCGCTTGCGTAAAACGCACATTTGCGTAATCGGTATCGGCGGCGTCGGTTCCTGGGCGGTGGAAGCTCTCGCCCGTTCCGGCATCGGCAAGATTACGATGATTGATATGGATGATATTTGTGTTACCAATATTAATCGCCAGATTCATGCCTTGAGCGGTAATATCGGGCAACTCaaa            >  1:801192/1‑251 (MQ=255)                                                                                                                                                                                                                        tAATGGCG‑‑CACATTGACAACTACGAACAACGCTTTGGCGGTATCGGCCGTTTATATGGCGCCGATGCTTTAGCGCGCTTGCGTAAAACGCACATTTGCGTAATCGGTATCGGCGGCGTCGGTTCCTGGGCGGTGGAAGCTCTCGCCCGTTCCGGCATCGGCAAGATTACGATGATTGATATGGATGATATTTGTGTTACCAATATTAATCGCCAGATTCATGCCTTGAGCGGTAATATCGGGCAACTCaaa            >  1:39839/1‑251 (MQ=255)                                                                                                                                                                                                                                    caTTGACAACTACGAACAACGCTTTGGCGGTATCGGCCGTTTATATGGCGCCGATGCTTTAGCGCGCTTGCGTAAAACGCACATTTGCGTAATCGGTATCGGCGGCGTCGGTTCCTGGGCGGTGGAAGct                                                                                                                           >  1:459454/1‑130 (MQ=255)                                                                                                                                                                                                                                    caTTGACAACTACGAACAACGCTTTGGCGGTATCGGCCGTTTATATGGCGCCGATGCTTTAGCGCGCTTGCGTAAAACGCACATTTGCGTAATCGGTATCGGCGGCGTCGGTTCCTGGGCGGTGGAAGCTCTCGCCCGTTCCGGCATCGGCAAGATTACGATGATTGATATGGATGATATTTGTGTTACCAATATTAATCGCCAGATTCATGCCTTGAGCGGTAATATCGGGCAACTCAAAACCGACATTa  >  1:551769/1‑251 (MQ=255)                                                                                                                                                                                                                                    caTTGACAACTACGAACAACGCTTTGGCGGTATCGGCCGTTTATATGGCGCCGATGCTTTAGCGCGCTTGCGTAAAACGCACATTTGCGTAATCGGTATCGGCGGCGTCGGTTCCTGGGCGGTGGAAGCTCTCGCCCGTTCCGGCATCGGCAAGATTACGATGATTGATATGGATGATATTTGTGTTACCAATATTAATCGCCAGATTCATGCCTTGAGCGGTAATATCGGGCAACTCAAAACCGACATTa  >  1:790408/1‑251 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                               GTGTGATTCCATTGGGTAGCGTGTTGCTGGTGGAGGTGCCGCAAATAGATAATGACGGCAACTGGACAGGGCAACATCAGTTACACCTCATGGTTGCGCTGGATGTGGGCGGTGCCGTGAACGGACACCATTTCGATTTATATCGCGGTATCGGCGATCAGGCGGGACATATTGCCGGCTTGTCGAAACATTACGGTCGCGTTTGGGTGTTACGTTAATGGCG‑‑CACATTGACAACTATGAACAACGCTTTGGCGGTATCGGCCGTTTATATGGCGCCGATGCTTTAGCGCGCTTGCGTAAAACGCACATTTGCGTAATCGGTATCGGCGGCGTCGGTTCCTGGGCGGTGGAAGCTCTCGCCCGTTCCGGCATCGGCAAGATTACGATGATTGATATGGATGATATTTGTGTTACCAATATTAATCGCCAGATTCATGCCTTGAGCGGTAATATCGGGCAACTCAAAACCGACATTA  >  1/1900885‑1901360

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑