Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,888,585	G→A	intergenic (‑4867/‑2488)	unknown ← / → unknown	tRNA‑Ser‑TGA/tRNA‑Gly‑GCC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,888,585	0	G	A	100.0%	227.9 / NA	79	intergenic (‑4867/‑2488)	unknown/unknown	tRNA‑Ser‑TGA/tRNA‑Gly‑GCC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (28/51);  total (28/51)

GGTTGCTACCGGTTTGCCATCTTTAAATAATAATAGTGTCGGGACACTACGCACGCCGAACTGTGCAGGGGTGGCCTGGTTATCGTCAATATTGATTTTCACAATTTTCACTTTACCATCAAATTCCACCGCCAAATCATCAAGGATCGGGGCAATCATTTTGCACGGTCCGCACCATGTAGCCCAAAAGTCTAATAATACAGGGACCTCTGAATTTAAAACGTCTGCAACGAATGTTGCATCGCTGGAATGTAATACTTCGCTCATTTTTTTTCCTTTTGTTACTGTTGTGTACTAGTGTTTAATTTCCTGCGCATAATAGCATAGCGCTATGCGAATAACACAAATTATTTTAATTGTTAAAAGCTAAGAAGACAAAACTACATTATCGCAAGGTTTGCCTTTCCCAATAGCATAAATATTAGACAGGGTAACATCGGCAATGCTGGTTAGCGCCTCTTCCGTTAGAAATGCCTGATGGCCTGTTAA  >  1/1888342‑1888830                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                      ggTTGCTACCGGTTTGCCATCTTTAAATAATAATAGTGTCGGGACACTACGCACGCCGAACTGTGCAGGGGTGGCCTGGTTATCGTCAATATTGATTTTCACAATTTTCACTTTACCATCAAATTCCACCGCCAAATCATCAAGGATCGGGGCAATCATTTTGCACGGTCCGCACCATGTAGCCCAAAAGTCTAATAATACAGGGACCTCTGAATTTAAAACGTCTGCAACGAATGTTGCATCACTGGAAt                                                                                                                                                                                                                                                >  1:747432/1‑251 (MQ=255)     gCTACCGGTTTGCCATCTTTAAATAATAATAGTGTCGGGACACTACGCACGCCGAACTGTGCAGGGGTGGCCTGGTTATCGTCAATATTGATTTTCACAATTTTCACTTTACCATCAAATTCCACCGCCAAATCATCAAGGATCGGGGCAATCATTTTGCACGGTCCGCACCATGTAGCCCAAAAGTCTAATAATACAGGGACCTCTGAATTTAAAACGTCTGCAACGAATGTTGCATCACTGGAATGTaa                                                                                                                                                                                                                                            >  1:685032/1‑251 (MQ=255)          cGGTTTGCCATCTTTAAATAATAATAGTGTCGGGACACTACGCACGCCGAACTGTGCAGGGGTGGCCTGGTTATCGTCAATATTGATTTTCACAATTTTCACTTTACCATCAAATTCCACCGCCAAATCATCAAGGATCGGGGCAATCATTTTGCACGGTCCGCACCATGTAGCCCAAAAGTCTAATAATACAGGGACCTCTGAATTTAAAACGTCTGCAACGAATGTTGCATCACTGGAATGTAATACtt                                                                                                                                                                                                                                       <  1:781672/251‑1 (MQ=255)                          aataataatAGTGTCGGGACACTACGCACGCCGAACTGTGCAGGGGTGGCCTGGTTATCGTCAATATTGATTTTCACAATTTTCACTTTACCATCAAATTCCACCGCCAAATCATCAAGGATCGGGGCAATCATTTTGCACGGTCCGCACCATGTAGCCCAAAAGTCTAATAATACAGGGACCTCTGAATTTAAAACGTCTGCAACGAATGTTGCATCACTGGAATGTAATACTTCGCTCATTTTTTTTc                                                                                                                                                                                                                        >  1:366520/1‑250 (MQ=255)                           ataataataGTGTCGGGACACTACGCACGCCGAACTGTGCAGGGGTGGCCTGGTTATCGTCAATATTGATTTTCACAATTTTCACTTTACCATCAAATTCCACCGCCAAATCATCAAGGATCGGGGCAATCATTTTGCACGGTCCGCACCATGTAGCCCAAAAGTCTAATAATACAGGGACCTCTGAATTTAAAACGTCTGCAACGAATGTTGCATCACTGGAATGTAATACTTCGCTCATTTTTTTTc                                                                                                                                                                                                                        >  1:228893/1‑249 (MQ=255)                            taataataGTGTCGGGACACTACGCACGCCGAACTGTGCAGGGGTGGCCTGGTTATCGTCAATATTGATTTTCACAATTTTCACTTTACCATCAAATTCCACCGCCAAATCATCAAGGATCGGGGCAATCATTTTGCACGGTCCGCACCATGTAGCCCAAAAGTCTAATAATACAGGGACCTCTGAATTTAAAACGTCTGCAACGAATGTTGCATCACTGGAATGTAATAc                                                                                                                                                                                                                                         <  1:533714/231‑1 (MQ=255)                            taataataGTGTCGGGACACTACGCACGCCGAACTGTGCAGGGGTGGCCTGGTTATCGTCAATATTGATTTTCACAATTTTCACTTTACCATCAAATTCCACCGCCAAATCATCAAGGATCGGGGCAATCATTTTGCACGGTCCGCACCATGTAGCCCAAAAGTCTAATAATACAGGGACCTCTGAATTTAAAACGTCTGCAACGAATGTTGCATCACTGGAATGTAATACTTCGCTCATTTTTTTTCCtt                                                                                                                                                                                                                     >  1:644901/1‑251 (MQ=255)                               taataGTGTCGGGACACTACGCACGCCGAACTGTGCAGGGGTGGCCTGGTTATCGTCAATATTGATTTTCACAATTTTCACTTTACCATCAAATTCCACCGCCAAATCATCAAGGATCGGGGCAATCATTTTGCACGGTCCGCACCATGTAGCCCAAAAGTCTAATAATACAGGGACCTCTGAATTTAAAACGTCTGCAACGAATGTTGCATCACTGGAATGTAATACTTCGCTCATTTTTTTTCCTTTTg                                                                                                                                                                                                                  >  1:517053/1‑251 (MQ=255)                               taataGTGTCGGGACACTACGCACGCCGAACTGTGCAGGGGTGGCCTGGTTATCGTCAATATTGATTTTCACAATTTTCACTTTACCATCAAATTCCACCGCCAAATCATCAAGGATCGGGGCAATCATTTTGCACGGTCCGCACCATGTAGCCCAAAAGTCTAATAATACAGGGACCTCTGAATTTAAAACGTCTGCAACGAATGTTGCATCACTGGAATGTAATACTTCGCTCATTTTTTTTCCTTTTg                                                                                                                                                                                                                  >  1:278262/1‑251 (MQ=255)                                     tgtCGGGACACTACGCACGCCGAACTGTGCAGGGGTGGCCTGGTTATCGTCAATATTGATTTTCACAATTTTCACTTTACCATCAAATTCCACCGCCAAATCATCAAGGATCGGGGCAATCATTTTGCACGGTCCGCACCATGTAGCCCAAAAGTCTAATAATACAGGGACCTCTGAATTTAAAACGTCTGCAACGAATGTTGCATCACTGGAATGTAATACTTCGCTCATTTTTTTTCCTTTTGTTAc                                                                                                                                                                                                              <  1:892091/249‑1 (MQ=255)                                      gtCGGGACACTACGCACGCCGAACTGTGCAGGGGTGGCCTGGTTATCGTCAATATTGATTTTCACAATTTTCACTTTACCATCAAATTCCACCGCCAAATCATCAAGGATCGGGGCAATCATTTTGCACGGTCCGCACCATGTAGCCCAAAAGTCTAATAATACAGGGACCTCTGAATTTAAAACGTCTGCAACGAATGTTGCATCACTGGAATGTAATACTTCGCTCATTTTTTTTCCTTTTGTTa                                                                                                                                                                                                               >  1:143779/1‑247 (MQ=255)                                       tCGGGACACTACGCACGCCGAACTGTGCAGGGGTGGCCTGGTTATCGTCAATATTGATTTTCACAATTTTCACTTTACCATCAAATTCCACCGCCAAATCATCAAGGATCGGGGCAATCATTTTGCACGGTCCGCACCATGTAGCCCAAAAGTCTAATAATACAGGGACCTCTGAATTTAAAACGTCTGCAACGAATGTTGCATCACTGGAATGTAATACTTCGCTCATTTTTTTTCCTTTTGTTAc                                                                                                                                                                                                              <  1:466179/247‑1 (MQ=255)                                       tCGGGACACTACGCACGCCGAACTGTGCAGGGGTGGCCTGGTTATCGTCAATATTGATTTTCACAATTTTCACTTTACCATCAAATTCCACCGCCAAATCATCAAGGATCGGGGCAATCATTTTGCACGGTCCGCACCATGTAGCCCAAAAGTCTAATAATACAGGGACCTCTGAATTTAAAACGTCTGCAACGAATGTTGCATCACTGGAATGTAATACTTCGCTCATTTTTTTTCCTTTTGTTACtgt                                                                                                                                                                                                           <  1:51200/250‑1 (MQ=255)                                          ggACACTACGCACGCCGAACTGTGCAGGGGTGGCCTGGTTATCGTCAATATTGATTTTCACAATTTTCACTTTACCATCAAATTCCACCGCCAAATCATCAAGGATCGGGGCAATCATTTTGCACGGTCCGCACCATGTAGCCCAAAAGTCTAATAATACAGGGACCTCTGAATTTAAAACGTCTGCAACGAATGTTGCATCACTGGAATGTAATACTTCGCTCATTTTTTTTCCTTTTGTTACTGTtgtg                                                                                                                                                                                                       >  1:216245/1‑251 (MQ=255)                                             cacTACGCACGCCGAACTGTGCAGGGGTGGCCTGGTTATCGTCAATATTGATTTTCACAATTTTCACTTTACCATCAAATTCCACCGCCAAATCATCAAGGATCGGGGCAATCATTTTGCACGGTCCGCACCATGTAGCCCAAAAGTCTAATAATACAGGGACCTCTGAATTTAAAACGTCTGCAACGAATGTTGCATCACTGGAATGTAATACTTCGCTCATTTTTTTTCCTTTTGTTACTGTTGTGTAc                                                                                                                                                                                                    <  1:807914/251‑1 (MQ=255)                                                tACGCACGCCGAACTGTGCAGGGGTGGCCTGGTTATCGTCAATATTGATTTTCACAATTTTCACTTTACCATCAAATTCCACCGCCAAATCATCAAGGATCGGGGCAATCATTTTGCACGGTCCGCACCATGTAGCCCAAAAGTCTAATAATACAGGGACCTCTGAATTTAAAACGTCTGCAACGAATGTTGCATCACTGGAATGTAATACTTCGCTCATTTTTTTTCCTTTTGTTACTGTTGTGTACTAg                                                                                                                                                                                                 <  1:593353/251‑1 (MQ=255)                                                tACGCACGCCGAACTGTGCAGGGGTGGCCTGGTTATCGTCAATATTGATTTTCACAATTTTCACTTTACCATCAAATTCCACCGCCAAATCATCAAGGATCGGGGCAATCATTTTGCACGGTCCGCACCATGTAGCCCAAAAGTCTAATAATACAGGGACCTCTGAATTTAAAACGTCTGCAACGAATGTTGCATCACTGGAATGTAATACTTCGCTCATTTTTTTTCCTTTTGTTACTGTTGTGTACTAg                                                                                                                                                                                                 <  1:475124/251‑1 (MQ=255)                                                  cgcacgcCGAACTGTGCAGGGGTGGCCTGGTTATCGTCAATATTGATTTTCACAATTTTCACTTTACCATCAAATTCCACCGCCAAATCATCAAGGATCGGGGCAATCATTTTGCACGGTCCGCACCATGTAGCCCAAAAGTCTAATAATACAGGGACCTCTGAATTTAAAACGTCTGCAACGAATGTTGCATCACTGGAATGTAATACTTCGCTCATTTTTTTTCCTTTTGTTACTGTTGTGTACTAgtg                                                                                                                                                                                               <  1:787015/251‑1 (MQ=255)                                                    cacgcCGAACTGTGCAGGGGTGGCCTGGTTATCGTCAATATTGATTTTCACAATTTTCACTTTACCATCAAATTCCACCGCCAAATCATCAAGGATCGGGGCAATCATTTTGCACGGTCCGCACCATGTAGCCCAAAAGTCTAATAATACAGGGACCTCTGAATTTAAAACGTCTGCAACGAATGTTGCATCACTGGAATGTAATACtt                                                                                                                                                                                                                                       <  1:595723/209‑1 (MQ=255)                                                     acgcCGAACTGTGCAGGGGTGGCCTGGTTATCGTCAATATTGATTTTCACAATTTTCACTTTACCATCAAATTCCACCGCCAAATCATCAAGGATCGGGGCAATCATTTTGCACGGTCCGCACCATGTAGCCCAAAAGTCTAATAATACAGGGACCTCTGAATTTAAAACGTCTGCAACGAATGTTGCATCACTGGAATGTAATAc                                                                                                                                                                                                                                         <  1:33985/206‑1 (MQ=255)                                                        ccGAACTGTGCAGGGGTGGCCTGGTTATCGTCAATATTGATTTTCACAATTTTCACTTTACCATCAAATTCCACCGCCAAATCATCAAGGATCGGGGCAATCATTTTGCACGGTCCGCACCATGTAGCCCAAAAGTCTAATAATACAGGGACCTCTGAATTTAAAACGTCTGCAACGAATGTTGCATCACTGGAATGTAATACTTCGCTCATTTTTTTTCCTTTTGTTACTGTTGTGTACTAGTGTTTAAt                                                                                                                                                                                         >  1:622711/1‑251 (MQ=255)                                                               gtgCAGGGGTGGCCTGGTTATCGTCAATATTGATTTTCACAATTTTCACTTTACCATCAAATTCCACCGCCAAATCATCAAGGATCGGGGCAATCATTTTGCACGGTCCGCACCATGTAGCCCAAAAGTCTAATAATACAGGGACCTCTGAATTTAAAACGTCTGCAACGAATGTTGCATCACTGGAATGTAATAc                                                                                                                                                                                                                                         <  1:726784/196‑1 (MQ=255)                                                               gtgCAGGGGTGGCCTGGTTATCGTCAATATTGATTTTCACAATTTTCACTTTACCATCAAATTCCACCGCCAAATCATCAAGGATCGGGGCAATCATTTTGCACGGTCCGCACCATGTAGCCCAAAAGTCTAATAATACAGGGACCTCTGAATTTAAAACGTCTGCAACGAATGTTGCATCACTGGAATGTAATAc                                                                                                                                                                                                                                         <  1:552026/196‑1 (MQ=255)                                                               gtgCAGGGGTGGCCTGGTTATCGTCAATATTGATTTTCACAATTTTCACTTTACCATCAAATTCCACCGCCAAATCATCAAGGATCGGGGCAATCATTTTGCACGGTCCGCACCATGTAGCCCAAAAGTCTAATAATACAGGGACCTCTGAATTTAAAACGTCTGCAACGAATGTTGCATCACTGGAATGTAATAc                                                                                                                                                                                                                                         >  1:694721/1‑196 (MQ=255)                                                               gtgCAGGGGTGGCCTGGTTATCGTCAATATTGATTTTCACAATTTTCACTTTACCATCAAATTCCACCGCCAAATCATCAAGGATCGGGGCAATCATTTTGCACGGTCCGCACCATGTAGCCCAAAAGTCTAATAATACAGGGACCTCTGAATTTAAAACGTCTGCAACGAATGTTGCATCACTGGAATGTAATACTTCGCTCATTTTTTTTCCTTTTGTTACTGTTGTGTAc                                                                                                                                                                                                    >  1:419001/1‑233 (MQ=255)                                                               gtgCAGGGGTGGCCTGGTTATCGTCAATATTGATTTTCACAATTTTCACTTTACCATCAAATTCCACCGCCAAATCATCAAGGATCGGGGCAATCATTTTGCACGGTCCGCACCATGTAGCCCAAAAGTCTAATAATACAGGGACCTCTGAATTTAAAACGTCTGCAACGAATGTTGCATCACTGGAATGTAATACTTCGCTCATTTTTTTTCCTTTTGTTACTGTTGTGTACTAGTGTTTAATTTCCTg                                                                                                                                                                                   >  1:284676/1‑250 (MQ=255)                                                               gtgCAGGGGTGGCCTGGTTATCGTCAATATTGATTTTCACAATTTTCACTTTACCATCAAATTCCACCGCCAAATCATCAAGGATCGGGGCAATCATTTTGCACGGTCCGCACCATGTAGCCCAAAAGTCTAATAATACAGGGACCTCTGAATTTAAAACGTCTGCAACGAATGTTGCATCACTGGAATGTAATACTTCGCTCATTTTTTTTCCTTTTGTTACTGTTGTGTACTAGTGTTTAATTTCCTg                                                                                                                                                                                   >  1:413990/1‑250 (MQ=255)                                                                  cAGGGGTGGCCTGGTTATCGTCAATATTGATTTTCACAATTTTCACTTTACCATCAAATTCCACCGCCAAATCATCAAGGATCGGGGCAATCATTTTGCACGGTCCGCACCATGTAGCCCAAAAGTCTAATAATACAGGGACCTCTGAATTTAAAACGTCTGCAACGAATGTTGCATCACTGGAATGTAATACTTCGCTCATTTTTTTTCCTTTTGTTACTGTTGTGTACTAGTGTTTAATTTCCTgcgc                                                                                                                                                                                <  1:697665/250‑1 (MQ=255)                                                                  cAGGGGTGGCCTGGTTATCGTCAATATTGATTTTCACAATTTTCACTTTACCATCAAATTCCACCGCCAAATCATCAAGGATCGGGGCAATCATTTTGCACGGTCCGCACCATGTAGCCCAAAAGTCTAATAATACAGGGACCTCTGAATTTAAAACGTCTGCAACGAATGTTGCATCACTGGAATGTAATACTTCGCTCATTTTTTTTCCTTTTGTTACTGTTGTGTACTAGTGTTTAATTTCCTgcgc                                                                                                                                                                                <  1:315670/250‑1 (MQ=255)                                                                          gCCTGGTTATCGTCAATATTGATTTTCACAATTTTCACTTTACCATCAAATTCCACCGCCAAATCATCAAGGATCGGGGCAATCATTTTGCACGGTCCGCACCATGTAGCCCAAAAGTCTAATAATACAGGGACCTCTGAGTTTAAAACGTCTGCAACGAATGTTGCATCACTGGAATGTAATACTTCGCTCATTTTTTTTCCTTTTGTTACTGTTGTGTACTAGTGTTTAATTTCCTGCGCATAatagca                                                                                                                                                                       >  1:650256/1‑251 (MQ=255)                                                                           ccTGGTTATCGTCAATATTGATTTTCACAATTTTCACTTTACCATCAAATTCCACCGCCAAATCATCAAGGATCGGGGCAATCATTTTGCACGGTCCGCACCATGTAGCCCAAAAGTCTAATAATACAGGGACCTCTGAATTTAAAACGTCTGCAACGAATGTTGCATCACTGGAATGTAATACTTCGCTCATTTTTTTTCCTTTTGTTACTGTTGTGTACTAGTGTTTAATTTCCTGCGCATAatagcat                                                                                                                                                                      <  1:866088/251‑1 (MQ=255)                                                                           ccTGGTTATCGTCAATATTGATTTTCACAATTTTCACTTTACCATCAAATTCCACCGCCAAATCATCAAGGATCGGGGCAATCATTTTGCACGGTCCGCACCATGTAGCCCAAAAGTCTAATAATACAGGGACCTCTGAATTTAAAACGTCTGCAACGAATGTTGCATCACTGGAATGTAATACTTCGCTCATTTTTTTTCCTTTTGTTACTGTTGTGTACTAGTGTTTAATTTCCTGCGCATAatagcat                                                                                                                                                                      <  1:361128/251‑1 (MQ=255)                                                                           ccTGGTTATCGTCAATATTGATTTTCACAATTTTCACTTTACCATCAAATTCCACCGCCAAATCATCAAGGATCGGGGCAATCATTTTGCACGGTCCGCACCATGTAGCCCAAAAGTCTAATAATACAGGGACCTCTGAATTTAAAACGTCTGCAACGAATGTTGCATCACTGGAATGTAATACTTCGCTCATTTTTTTTCCTTTTGTTACTGTTGTGTACTAGTGTTTAATTTCCTGCGCATAatagcat                                                                                                                                                                      <  1:146399/251‑1 (MQ=255)                                                                               gTTATCGTCAATATTGATTTTCACAATTTTCACTTTACCATCAAATTCCACCGCCAAATCATCAAGGATCGGGGCAATCATTTTGCACGGTCCGCACCATGTAGCCCAAAAGTCTAATAATACAGGGACCTCTGAATTTAAAACGTCTGCAACGAATGTTGCATCACTGGAATGTAATAc                                                                                                                                                                                                                                         >  1:589045/1‑180 (MQ=255)                                                                               gTTATCGTCAATATTGATTTTCACAATTTTCACTTTACCATCAAATTCCACCGCCAAATCATCAAGGATCGGGGCAATCATTTTGCACGGTCCGCACCATGTAGCCCAAAAGTCTAATAATACAGGGACCTCTGAATTTAAAACGTCTGCAACGAATGTTGCATCACTGGAATGTAATACTTCGCTCATTTTTTTTCCTTTTGTTACTGTTGTGTACTAGTGTTTAATTTCCTGCGCATAatagcatagc                                                                                                                                                                   <  1:359971/250‑1 (MQ=255)                                                                                ttATCGTCAATATTGATTTTCACAATTTTCACTTTACCATCAAATTCCACCGCCAAATCATCAAGGATCGGGGCAATCATTTTGCACGGTCCGCACCATGTAGCCCAAAAGTCTAATAATACAGGGACCTCTGAATTTAAAACGTCTGCAACGAATGTTGCATCACTGGAATGTAATACTTCGCTCATTTTTTTTCCTTTTGTTACTGTTGTGTACTAGTGTTTAATTTCCTGCGCATAATAGCATAgcgc                                                                                                                                                                 <  1:530749/251‑1 (MQ=255)                                                                                ttATCGTCAATATTGATTTTCACAATTTTCACTTTACCATCAAATTCCACCGCCAAATCATCAAGGATCGGGGCAATCATTTTGCACGGTCCGCACCATGTAGCCCAAAAGTCTAATAATACAGGGACCTCTGAATTTAAAACGTCTGCAACGAATGTTGCATCACTGGAATGTAATACTTCGCTCATTTTTTTTCCTTTTGTTACTGTTGTGTACTAGTGTTTAATTTCCTGCGCATAATAGCATAgcgc                                                                                                                                                                 <  1:537783/251‑1 (MQ=255)                                                                                ttATCGTCAATATTGATTTTCACAATTTTCACTTTACCATCAAATTCCACCGCCAAATCATCAAGGATCGGGGCAATCATTTTGCACGGTCCGCACCATGTAGCCCAAAAGTCTAATAATACAGGGACCTCTGAATTTAAAACGTCTGCAACGAATGTTGCATCACTGGAATGTAATACTTCGCTCATTTTTTTTCCTTTTGTTACTGTTGTGTACTAGTGTTTAATTTCCTGCGCATAATAGCATAgcgc                                                                                                                                                                 <  1:614669/251‑1 (MQ=255)                                                                                ttATCGTCAATATTGATTTTCACAATTTTCACTTTACCATCAAATTCCACCGCCAAATCATCAAGGATCGGGGCAATCATTTTGCACGGTCCGCACCATGTAGCCCAAAAGTCTAATAATACAGGGACCTCTGAATTTAAAACGTCTGCAACGAATGTTGCATCACTGGAATGTAATACTTCGCTCATTTTTTTTCCTTTTGTTACTGTTGTGTACTAGTGTTTAATTTCCTGCGCATAATAGCATAgcgc                                                                                                                                                                 <  1:163819/251‑1 (MQ=255)                                                                                      tCAATATTGATTTTCACAATTTTCACTTTACCATCAAATTCCACCGCCAAATCATCAAGGATCGGGGCAATCATTTTGCACGGTCCGCACCATGTAGCCCAAAAGTCTAATAATACAGGGACCTCTGAATTTAAAACGTCTGCAACGAATGTTGCATCACTGGAATGTAATACTTCGCTCATTTTTTTTCCTTTTGTTACTGTTGTGTACTAGTGTTTAATTTCCTGCGCATAATAGCATAGCGCTATGc                                                                                                                                                            <  1:287031/250‑1 (MQ=255)                                                                                         atatTGATTTTCACAATTTTCACTTTACCATCAAATTCCACCGCCAAATCATCAAGGATCGGGGCAATCATTTTGCACGGTCCGCACCATGTAGCCCAAAAGTCTAATAATACAGGGACCTCTGAATTTAAAACGTCTGCAACGAATGTTGCATCACTGGAATGTAATACTTCGCTCATTTTTTTTCCTTTTGTTACTGTTGTGTACTAGTGTTTAATTTCCTGCGCataat                                                                                                                                                                           <  1:431924/232‑1 (MQ=255)                                                                                         atatTGATTTTCACAATTTTCACTTTACCATCAAATTCCACCGCCAAATCATCAAGGATCGGGGCAATCATTTTGCACGGTCCGCACCATGTAGCCCAAAAGTCTAATAATACAGGGACCTCTGAATTTAAAACGTCTGCAACGAATGTTGCATCACTGGAATGTAATACTTCGCTCATTTTTTTTCCTTTTGTTACTGTTGTGTACTAGTGTTTAATTTCCTGCGCATAatagcat                                                                                                                                                                      <  1:540048/237‑1 (MQ=255)                                                                                         atatTGATTTTCACAATTTTCACTTTACCATCAAATTCCACCGCCAAATCATCAAGGATCGGGGCAATCATTTTGCACGGTCCGCACCATGTAGCCCAAAAGTCTAATAATACAGGGACCTCTGAATTTAAAACGTCTGCAACGAATGTTGCATCACTGGAATGTAATACTTCGCTCATTTTTTTTCCTTTTGTTACTGTTGTGTACTAGTGTTTAATTTCCTGCGCATAatag                                                                                                                                                                         >  1:553184/1‑234 (MQ=255)                                                                                                   tCACAATTTTCACTTTACCATCAAATTCCACCGCCAAATCATCAAGGATCGGGGCAATCATTTTGCACGGTCCGCACCATGTAGCCCAAAAGTCTAATAATACAGGGACCTCTGAATTTAAAACGTCTGCAACGAATGTTGCATCACTGGAATGTAATACTTCGCTCATTTTTTTTCCTTTTGTTACTGTTGTGTACTAGTGTTTAATTTCCTGCGCATAGTAGCATAGCGCTATGCGAATAac                                                                                                                                                     >  1:396859/1‑244 (MQ=255)                                                                                                   tCACAATTTTCACTTTACCATCAAATTCCACCGCCAAATCATCAAGGATCGGGGCAATCATTTTGCACGGTCCGCACCATGTAGCCCAAAAGTCTAATAATACAGGGACCTCTGAATTTAAAACGTCTGCAACGAATGTTGCATCACTGGAATGTAATACTTCGCTCATTTTTTTTCCTTTTGTTACTGTTGTGTACTAGTGTTTAATTTCCTGCGCATAATAGCATAGCGCTATGCGAATAACACAAatt                                                                                                                                              <  1:358883/251‑1 (MQ=255)                                                                                                        aTTTTCACTTTACCATCAAATTCCACCGCCAAATCATCAAGGATCGGGGCAATCATTTTGCACGGTCCGCACCATGTAGCCCAAAAGTCTAATAATACAGGGACCTCTGAATTTAAAACGTCTGCAACGAATGTTGCATCACTGGAATGTAATACTTCGCTCATTTTTTTTCCTTTTGTTACTGTTGTGTACTAGTGTTTAATTTCCTGCGCATAATAGCATAGCGCTATGCGAATAACACAAatt                                                                                                                                              <  1:130918/246‑1 (MQ=255)                                                                                                                            ttCCACCGCCAAATCATCAAGGATCGGGGCAATCATTTTGCACGGTCCGCACCATGTAGCCCAAAAGTCTAATAATACAGGGACCTCTGAATTTAAAACGTCTGCAACGAATGTTGCATCACTGGAATGTAATACTTCGCTCATTTTTTTTCCTTTTGTTACTGTTGTGTACTAGTGTTTAATTTCCTGCGCATAATAGCATAGCGCTATGCGAATAACACAAATTATTTTAATTGTTAAAAGCTaagaag                                                                                                                     <  1:558553/251‑1 (MQ=255)                                                                                                                                        atcatcAAGGATCGGGGCAATCATTTTGCACGGTCCGCACCATGTAGCCCAAAAGTCTAATAATACAGGGACCTCTGAATTTAAAACGTCTGCAACGAATGTTGCATCACTGGAATGTAATACTTCGCTCATTTTTTTTCCTTTTGtt                                                                                                                                                                                                                >  1:754599/1‑148 (MQ=255)                                                                                                                                           atcaAGGATCGGGGCAATCATTTTGCACGGTCCGCACCATGTAGCCCAAAAGTCTAATAATACAGGGACCTCTGAATTTAAAACGTCTGCAACGAATGTTGCATCACTGGAATGTAATACTTCGCTCATTTTTTTTCCTTTTGTTACTGTTGTGTACTAGTGTTTAATTTCCTGCGCATAatag                                                                                                                                                                         <  1:709715/184‑1 (MQ=255)                                                                                                                                            tcaAGGATCGGGGCAATCATTTTACACGGTCCGCACCATGTAGCCCAAAAGTCTAATAATACAGGGACCTCTGAATTTAAAACGTCTGCAACGAATGTTGCATCACTGGAATGTAATACTTCGCTCATTTTTTTTCCTTTTGTTACTGTTGTGTACTAGTGTTTAATTTCCTGCGCataat                                                                                                                                                                           <  1:550830/181‑1 (MQ=255)                                                                                                                                                      gggCAATCATTTTGCACGGTCCGCACCATGTAGCCCAAAAGTCTAATAATACAGGGACCTCTGAATTTAAAACGTCTGCAACGAATGTTGCATCACTGGAATGTAATACTTCGCTCATTTTTTTTCCTTTTGTTACTGTTGTGTACTAGTGTTTAATTTCCTGCGCATAatagcat                                                                                                                                                                      >  1:850160/1‑176 (MQ=255)                                                                                                                                                         cAATCATTTTGCACGGTCCGCACCATGTAGCCCAAAAGTCTAATAATACAGGGACCTCTGAATTTAAAACGTCTGCAACGAATGTTGCATCACTGGAATGTAATACTTCGCTCATTTTTTTTCCTTTTGTTACTGTTGTGTACTAGTGTTTAATTTCCTGCGCATAATAGCATAGCGCTATGCGAATAACACAAATTATTTTAATTGTTAAAAGCTAAGAAGACAAAACTACATTATCGCAAGGTTTGcc                                                                                         <  1:855270/250‑1 (MQ=255)                                                                                                                                                         cAATCATTTTGCACGGTCCGCACCATGTAGCCCAAAAGTCTAATAATACAGGGACCTCTGAATTTAAAACGTCTGCAACGAATGTTGCATCACTGGAATGTAATACTTCGCTCATTTTTTTTCCTTTTGTTACTGTTGTGTACTAGTGTTTAATTTCCTGCGCATAATAGCATAGCGCTATGCGAATAACACAAATTATTTTAATTGTTAAAAGCTAAGAAGACAAAACTACATTATCGCAAGGTTTGcc                                                                                         <  1:17135/250‑1 (MQ=255)                                                                                                                                                         cAATCATTTTGCACGGTCCGCACCATGTAGCCCAAAAGTCTAATAATACAGGGACCTCTGAATTTAAAACGTCTGCAACGAATGTTGCATCACTGGAATGTAATACTTCGCTCATTTTTTTTCCTTTTGTTACTGTTGTGTACTAGTGTTTAATTTCCTGCGCATAATAGCATAGCGCTATGCGAATAACACAAATTATTTTAATTGTTAAAAGCTAAGAAGACAAAACTACATTATCGCAAGGTTTGCCt                                                                                        <  1:44978/251‑1 (MQ=255)                                                                                                                                                              aTTTTGCACGGTCCGCACCATGTAGCCCAAAAGTCTAATAATACAGGGACCTCTGAATTTAAAACGTCTGCAACGAATGTTGCATCACTGGAATGTAATACTTCGCTCATTTTTTTTCCTTTTGTTACTGTTGTGTACTAGTGTTTAATTTCCTGCGCATAATAGCATAGCGCTATGCGAATAACACAAATTATTTTAATTGTTAAAAGCTAAGAAGACAAAACTACATTATCGCAAGGTTTGCCTTTccc                                                                                   <  1:603717/251‑1 (MQ=255)                                                                                                                                                                       ggTCCGCACCATGTAGCCCAAAAGTCTAATAATACAGGGACCTCTGAATTTAAAACGTCTGCAACGAATGTTGCATCACTGGAATGTAATACTTCGCTCATTTTTTTTCCTTTTGTTACTGTTGTGTACTAGTGTTTAATTTCCTGCGCATAATAGCATAGCGCTATGCGAATAACACAAATTATTTTAATTGTTAAAAGCTAAGAAGACAAAACTACATTATCGCAAGGTTTGCCTTTCCCAATAGCATa                                                                          >  1:786662/1‑251 (MQ=255)                                                                                                                                                                        gTCCGCACCATGTAGCCCAAAAGTCTAATAATACAGGGACCTCTGAATTTAAAACGTCTGCAACGAATGTTGCATCACTGGAATGTAATACTTCGCTCATTTTTTTTCCTTTTGTTACTGTTGTGTACTAGTGTTTAATTTCCTGCGCATAatagcatagc                                                                                                                                                                   <  1:276044/161‑1 (MQ=255)                                                                                                                                                                        gTCCGCACCATGTAGCCCAAAAGTCTAATAATACAGGGACCTCTGAATTTAAAACGTCTGCAACGAATGTTGCATCACTGGAATGTAATACTTCGCTCATTTTTTTTCCTTTTGTTACTGTTGTGTACTAGTGTTTAATTTCCTGCGCATAATAGCATAGCGCTATGCGAATAACACAAATTATTTTAATTGTTAAAAGCTAAGAAGACAAAACTACATTATCGCAAGGTTTGCCTTTCCCAATAGCATaa                                                                         >  1:822613/1‑251 (MQ=255)                                                                                                                                                                        gTCCGCACCATGTAGCCCAAAAGTCTAATAATACAGGGACCTCTGAATTTAAAACGTCTGCAACGAATGTTGCATCACTGGAATGTAATACTTCGCTCATTTTTTTTCCTTTTGTTACTGTTGTGTACTAGTGTTTAATTTCCTGCGCATAATAGCATAGCGCTATGCGAATAACACAAATTATTTTAATTGTTAAAAGCTAAGAAGACAAAACTACATTATCGCAAGGTTTGCCTTTCCCAATAGCATaa                                                                         >  1:581121/1‑251 (MQ=255)                                                                                                                                                                                 aTGTAGCCCAAAAGTCTAATAATACAGGGACCTCTGAATTTAAAACGTCTGCAACGAATGTTGCATCACTGGAATGTAATACTTCGCTCATTTTTTTTCCTTTTGTTTCTGTTGTGTACTAGTGTTTAATTTCCTGCGCATAATAGCATAGCGCTATGCGAATAACACAAATTATTTTAATTGTTAAAAGCTAAGAAGACAAAACTACATTATCGCAAGGTTTGCCTTTCCCAATAGCATAAATATTAGAc                                                                <  1:888025/251‑1 (MQ=255)                                                                                                                                                                                 aTGTAGCCCAAAAGTCTAATAATACAGGGACCTCTGAATTTAAAACGTCTGCAACGAATGTTGCATCACTGGAATGTAATACTTCGCTCATTTTTTTTCCTTTTGTTACTGTTGTGTAc                                                                                                                                                                                                    <  1:668642/119‑1 (MQ=255)                                                                                                                                                                                 aTGTAGCCCAAAAGTCTAATAATACAGGGACCTCTGAATTTAAAACGTCTGCAACGAATGTTGCATCACTGGAATGTAATACTTCGCTCATTTTTTTTCCTTTTGTTACTGTTGTGTACTAGTGTTTAATTTCCTGCGCATAATAGCATAGCGCTATGCGAATAACACAAATTATTTTAATTGTTAAAAGCTAAGAAGACAAAACTACATTATCGCAAGGTTTGCCTTTCCCAATAGCATAAATATTAGa                                                                 >  1:395648/1‑250 (MQ=255)                                                                                                                                                                                 aTGTAGCCCAAAAGTCTAATAATACAGGGACCTCTGAATTTAAAACGTCTGCAACGAATGTTGCATCACTGGAATGTAATACTTCGCTCATTTTTTTTCCTTTTGTTACTGTTGTGTACTAGTGTTTAATTTCCTGCGCATAATAGCATAGCGCTATGCGAATAACACAAATTATTTTAATTGTTAAAAGCTAAGAAGACAAAACTACATTATCGCAAGGTTTGCCTTTCCCAATAGCATAAATATTAGAc                                                                <  1:554224/251‑1 (MQ=255)                                                                                                                                                                                 aTGTAGCCCAAAAGTCTAATAATACAGGGACCTCTGAATTTAAAACGTCTGCAACGAATGTTGCATCACTGGAATGTAATACTTCGCTCATTTTTTTTCCTTTTGTTACTGTTGTGTACTAGTGTTTAATTTCCTGCGCATAATAGCATAGCGCTATGCGAATAACACAAATTATTTTAATTGTTAAAAGCTAAGAAGACAAAACTACATTATCGCAAGGTTTGCCTTTCCCAATAGCATAAATATTAGAc                                                                <  1:870243/251‑1 (MQ=255)                                                                                                                                                                                 aTGTAGCCCAAAAGTCTAATAATACAGGGACCTCTGAATTTAAAACGTCTGCAACGAATGTTGCATCACTGGAATGTAATACTTCGCTCATTTTTTTTCCTTTTGTTACTGTTGTGTACTAGTGTTTAATTTCCTGCGCATAATAGCATAGCGCTATGCGAATAACACAAATTATTTTAATTGTTAAAAGCTAAGAAGACAAAACTACATTATCGCAAGGTTTGCCTTTCCCAATAGCATAAATATTAGAc                                                                <  1:850470/251‑1 (MQ=255)                                                                                                                                                                                 aTGTAGCCCAAAAGTCTAATAATACAGGGACCTCTGAATTTAAAACGTCTGCAACGAATGTTGCATCACTGGAATGTAATACTTCGCTCATTTTTTTTCCTTTTGTTACTGTTGTGTACTAGTGTTTAATTTCCTGCGCATAATAGCATAGCGCTATGCGAATAACACAAATTATTTTAATTGTTAAAAGCTAAGAAGACAAAACTACATTATCGCAAGGTTTGCCTTTCCCAATAGCATAAATATTAGAc                                                                <  1:512675/251‑1 (MQ=255)                                                                                                                                                                                           aaaGTCTAATAATACAGGGACCTCTGAATTTAAAACGTCTGCAACGAATGTTGCATCACTGGAATGTAATACTTCGCTCATTTTTTTTCCTTTTGTTACTGTTGTGTACTAGTGTTTAATTTCCTGCGCATAATAGCATAGCGCTATGCGAATAACACAAATTATTTTAATTGTTAAAAGCTAAGAAGACAAAACTACATTATCGCAAGGTTTGCCTTTCCCAATAGCATAAATATTAGACAGGGTAGCAt                                                      <  1:602810/251‑1 (MQ=255)                                                                                                                                                                                              gTCTAATAATACAGGGACCTCTGAATTTAAAACGTCTGCAACGAATGTTGCATCACTGGAATGTAATACTTCGCTCATTTTTTTTCCTTTTGTTACTGTTGTGTACTAg                                                                                                                                                                                                 <  1:208265/109‑1 (MQ=255)                                                                                                                                                                                                cTAATAATACAGGGACCTCTGAATTTAAAACGTCTGCAACGAATGTTGCATCACTGGAATGAAATCCTTCGCTCATTTTTTTTCCTTTTGTTACTGTTGTGTACTAGTGTTTAATTTCCTGCGCATAATAGCATAGCGCTATGCGAATAACACAAATTATTTTAATTGTTAAAAGCTAAGAAGACAAAACTACATTATCGCAAGGTTTGCCTTTCCCAATAGCATAAATATTAGACAGGGTAACATCGGCa                                                 >  1:550634/1‑251 (MQ=255)                                                                                                                                                                                                 taataaTACAGGGACCTCTGAATTTAAAACGTCTGCAACGAATGTTGCATCACTGGAATGTAATACTTCGCTCATTTTTTTTCCTTTTGTTACTGTTGTGTACTAGTGTTTAATTTCCTGCGCATAatagcat                                                                                                                                                                      <  1:191327/133‑1 (MQ=255)                                                                                                                                                                                                 taataaTACAGGGACCTCTGAATTTAAAACGTCTGCAACGAATGTTGCATCACTGGAATGTAATACTTCGCTCATTTTTTTTCCTTTTGTTACTGTTGTGTACTAGTGTTTAATTTCCTGCGCATAATAGCATAGCGCTATGc                                                                                                                                                            >  1:703380/1‑143 (MQ=255)                                                                                                                                                                                                   ataataCAGGGACCTCTGAATTTAAAACGTCTGCAACGAATGTTGCATCACTGGAATGTAATACTTCGCTCATTTTTTTTCCTTTTGTTACTGTTGTGTACTAGTGTTTAATTTCCTGCGCATAatagcatag                                                                                                                                                                    <  1:737294/133‑1 (MQ=255)                                                                                                                                                                                                    taataCAGGGACCTCTGAATTTAAAACGTCTGCAACGAATGTTGCATCACTGGAATGTAATACTTCGCTCATTTTTTTTCCTTTTGTTACTGTTGTGTACTAGTGTTTAATTTCCTGCGCATAATAGCATAGCGCTATGCGAATAACACAAATTATTTTAATTGTTAAAAGCTAAGAAGACAAAACTACATTATCGCAAGGTTTGCCTTTCCCAATAGCATAAATATTAGACAGGGTAACATCGGCAATGc                                             <  1:867209/251‑1 (MQ=255)                                                                                                                                                                                                      ataCAGGGACCTCTGAATTTAAAACGTCTGCAACGAATGTTGCATCACTGGAATGTAATACTTCGCTCATTTTTTTTCCTTTTGTTACTGTTGTGTACTAGTGTTTAATTTCCTGCGCATAATAGCATAGCGCTATGCGAATAACACAAATTATTTTAATTGTTAAAAGCTAAGAAGACAAAACTACATTATCGCAAgg                                                                                               <  1:519056/199‑1 (MQ=255)                                                                                                                                                                                                      ataCAGGGACCTCTGAATTTAAAACGTCTGCAACGAATGTTGCATCACTGGAATGTAATACTTCGCTCATTTTTTTTCCTTTTGTTACTGTTGTGTACTAGTGTTTAATTTCCTGCGCATAATAGCATAGCGCTATGCGAATAACACAAATTATTTTAATTGTTAAAAGCTAAGAAGACAAAACTACATTATCGCAAGGTTTGCCTTTCCCAATAGCATAAatat                                                                     <  1:137683/225‑1 (MQ=255)                                                                                                                                                                                                                ctctGAATTTAAAACGTCTGCAACGAATGTTGCATCACTGGAATGTAATACTTCGCTCATTTTTTTTCCTTTTGTTACTGTTGTGTACTAGTGTTTAATTTCCTGCGCATAATAGCATAGCGCTATGCGAATAACACAAATTATTTTAATTGTTAAAAGCTAAGAAGACAAAACTACATTATCGCAAGGTTTGCCTTTCCCAATAGCATAAATATTAGACAGGGTAACATCGGCAATGCTGGTTAgcgc                                   >  1:2128/1‑249 (MQ=255)                                                                                                                                                                                                                      aTTTAAAACGTCTGCAACGAATGTTGCATCACTGGAATGTAATACTTCGCTCATTTTTTTTCCTTTTGTTACTGTTGTGTACTAGTGTTTAATTTCCTGCGCATAATAGCATAgcgc                                                                                                                                                                 <  1:506118/117‑1 (MQ=255)                                                                                                                                                                                                                      aTTTAAAACGTCTGCAACGAATGTTGCATCACTGGAATGTAATACTTCGCTCATTTTTTTTCCTTTTGTTACTGTTGTGTACTAGTGTTTAATTTCCTGCGCATAATAGCATAGCGCTATGCGAATAACACAAATTATTTTAATTGTTAAAAGCTAAGAAGACAAAACTACATTATCGCAAGGTTTGCCTTTCCCAATAGCATAAATATTAGACAGGGt                                                           <  1:35271/219‑1 (MQ=255)                                                                                                                                                                                                                                               gCATCACTGGAATGTAATACTTCGCTCATTTTTTTTCCTTTTGTTACTGTTGTGTACTAGTGTTTAATTTCCTGCGCATAATAGCATAGCGCTATGCGAATAACACAAATTATTTTAATTGTTAAAAGCTAAGAAGACAAAACTACATTATCGCAAGGTTTGCCTTTCCCAATAGCATAAATATTAGACAGGGTAACATCGGCAATGCTGGTTAGCGCCTCTTCCGTTAGAAATGCCTGATGGCCTGTtaa  >  1:595460/1‑251 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                      GGTTGCTACCGGTTTGCCATCTTTAAATAATAATAGTGTCGGGACACTACGCACGCCGAACTGTGCAGGGGTGGCCTGGTTATCGTCAATATTGATTTTCACAATTTTCACTTTACCATCAAATTCCACCGCCAAATCATCAAGGATCGGGGCAATCATTTTGCACGGTCCGCACCATGTAGCCCAAAAGTCTAATAATACAGGGACCTCTGAATTTAAAACGTCTGCAACGAATGTTGCATCGCTGGAATGTAATACTTCGCTCATTTTTTTTCCTTTTGTTACTGTTGTGTACTAGTGTTTAATTTCCTGCGCATAATAGCATAGCGCTATGCGAATAACACAAATTATTTTAATTGTTAAAAGCTAAGAAGACAAAACTACATTATCGCAAGGTTTGCCTTTCCCAATAGCATAAATATTAGACAGGGTAACATCGGCAATGCTGGTTAGCGCCTCTTCCGTTAGAAATGCCTGATGGCCTGTTAA  >  1/1888342‑1888830

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑