Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,865,616	A→G	intergenic (‑29953/+18013)	unknown ← / ← unknown	tRNA‑Lys‑TTT/tRNA‑Ser‑TGA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,865,616	0	A	G	100.0%	234.1 / NA	76	intergenic (‑29953/+18013)	unknown/unknown	tRNA‑Lys‑TTT/tRNA‑Ser‑TGA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (39/37);  total (39/37)

TGACGGGAATTTTAAAAGATATTATTGTTGTCAGCGGAGGCGTGATTGGTGCGGCCTCGCCTTCGGTTTTAAAGATGTGAAAGTCTTAATTGAGATCATACGCTCTGTACTTCGGCAGTAGGAAAATATTGCCGATGATCGGGTGTTGTAGCGTTATCAACAAAAACGAAAAACCGATAATTTACTCATGCAATCGGGCATGGATCTGTTTTATAAGGCGTTTAAGGAAAAGATTTTACCATTGAAAATGCTACGAAATATCGCATTAGTAGCGGCAGATAAAACCCCGTTGTTGAAAAAACAAGCCTTGCGTTACGCCTTGGGATTATAATCTGCCGTTGATAAATCTAAATATTCGATTATTGCCAATATAAAATCTTTTAATTTACATGCTCAATGTTTAAGTTATATACGATATAAACTAATCATTCCAATAAAGAACCGCACTCTGAAGCTCAAAGTGCGGTCATTTAT  >  1/1865379‑1865852                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                             tGACGGGAATTTTAAAAGATATTATTGTTGTCAGCGGAGGCGTGATTGGTGCGGCCTCGCCTTCGGTTTTAAAGATGTGAAAGTCTTAATTGAGATCATACGCTCTGTACTTCGGCAGTAGGAAAATATTGCCGATGATCGGGTGTTGTAGCGTTATCAACAAAAACGAAAAACCGATAATTTACTCATGCAATCGGGCATGGATCTGTTTTATAAGGCGTTTAAGGAAAAGATTTTGCCATTGAAAATGc                                                                                                                                                                                                                                 <  1:521826/251‑1 (MQ=255)           tttAAAAGATATTATTGTTGTCAGCGGAGGCGTGATTGGTGCGGCCTCGCCTTCGGTTTTAAAGATGTGAAAGTCTTAATTGAGATCATACGCTCTGTACTTCGGCAGTAGGAAAATATTGCCGATGATCGGGTGTTGTAGCGTTATCAACAAAAACGAAAAACCGATAATTTACTCATGCAATCGGGCATGGATCTGTTTTATAAGGCGTTTAAGGAAAAGATTTTGCCATTGAAAATGCTACGAAatat                                                                                                                                                                                                                       <  1:713699/251‑1 (MQ=255)                          tgttgtCAGCGGAGGCGTGATTGGTGCGGCCTCGCCTTCGGTTTTAAAGATGTGAAAGTCTTAATTGAGATCATACGCTCTGTACTTCGGCAGTAGGAAAATATTGCCGATGATCGGGTGTTGTAGCGTTATCAACAAAAACGAAAAACCGATAATTTACTCATGCAATCGGGCATGGATCTGTTTTATAAGGCGTTTAAGGAAAAGATTTTGCCATTGAAAATGCTACGAAATATCGCATTAGTAGCGGc                                                                                                                                                                                                        <  1:115199/251‑1 (MQ=255)                                cAGCGGAGGCGTGATTGGTGCGGCCTCGCCTTCGGTTTTAAAGATGTGAAAGTCTTAATTGAGATCATACGCTCTGTACTTCGGCAGTAGGAAAATATTGCCGATGATCGGGTGTTGTAGCGTTATCAACAAAAACGAAAAACCGATAATTTACTCATGCAATCGGGCATGGATCTGTTTTATAAGGCGTTTAAGGAAAAGATTTTGCCATTGAAAATGCTACGAAATATCGCATTAGTAGCGGCAGATaa                                                                                                                                                                                                  >  1:211657/1‑251 (MQ=255)                                                    cGGCCTCGCCTTCGGTTTTAAAGATGTGAAAGTCTTAATTGAGATCATACGCTCTGTACTTCGGCAGTAGGAAAATATTGCCGATGATCGGGTGTTGTAGCGTTATCAACAAAAACGAAAAACCGATAATTTACTCATGCAATCGGGCATGGATCTGTTTTATAAGGCGTTTAAGGAAAAGATTTTGCCATTGAAAATGCTACGAAATATCGCATTAGTAGCGGCAGATAAAACCCCGTTGTTGAAAAAAc                                                                                                                                                                              <  1:655145/251‑1 (MQ=255)                                                                  gTTTTAAAGATGTGAAAGTCTTAATTGAGATCATACGCTCTGTACTTCGGCAGTAGGAAAATATTGCCGATGATCGGGTGTTGTAGCGTTATCAACAAAAACGAAAAACCGATAATTTACTCATGCAATCGGGCATGGATCTGTTTTATAAGGCGTTTAAGGAAAAGATTTTGCCATTGAAAATGCTACGAAATATCGCATTAGTAGCGGCAGATAAAACCCCGTTGTTGAAAAAACAAGCCTTGCGTTa                                                                                                                                                                 >  1:486538/1‑250 (MQ=255)                                                                           aTGTGAAAGTCTTAATTGAGATCATACGCTCTGTACTTCGGCAGTAGGAAAATATTGCCGATGATCGGGTGTTGTAGCGTTATCAACAAAAACGAAAAACCGATAATTTACTCATGCAATCGGGCATGGATCTGTTTTATAAGGCGTTTAAGGAAAAGATTTTGCCATTGAAAATGCTACGAAATATCGCATTAGTAGCGGCAGATAAAACCCCGTTGTTGAAAAAACAAGCCTTGCGTTACGCCTTGGGa                                                                                                                                                       <  1:668874/251‑1 (MQ=255)                                                                           aTGTGAAAGTCTTAATTGAGATCATACGCTCTGTACTTCGGCAGTAGGAAAATATTGCCGATGATCGGGTGTTGTAGCGTTATCAACAAAAACGAAAAACCGATAATTTACTCATGCAATCGGGCATGGATCTGTTTTATAAGGCGTTTAAGGAAAAGATTTTGCCATTGAAAATGCTACGAAATATCGCATTAGTAGCGGCAGATAAAACCCCGTTGTTGAAAAAACAAGCCTTGCGTTACGCCTTGGGa                                                                                                                                                       <  1:263615/251‑1 (MQ=255)                                                                            tgtgAAAGTCTTAATTGAGATCATACGCTCTGTACTTCGGCAGTAGGAAAATATTGCCGATGATCGGGTGTTGTAGCGTTATCAACAAAAACGAAAAACCGATAATTTACTCATGCAATCGGGCATGGATCTGTTTTATAAGGCGTTTAAGGAAAAGATTTTGCCATTGAAAATGCTACGAAATATCGCATTAGTAGCGGCAGATAAAACCCCGTTGTTGAAAAAACAAGCCTTGCGTTACGCCTTGGGAt                                                                                                                                                      >  1:640256/1‑251 (MQ=255)                                                                            tgtgAAAGTCTTAATTGAGATCATACGCTCTGTACTTCGGCAGTAGGAAAATATTGCCGATGATCGGGTGTTGTAGCGTTATCAACAAAAACGAAAAACCGATAATTTACTCATGCAATCGGGCATGGATCTGTTTTATAAGGCGTTTAAGGAAAAGATTTTGCCATTGAAAATGCTACGAAATATCGCATTAGTAGCGGCAGATAAAACCCCGTTGTTGAAAAAACAAGCCTTGCGTTACGCCTTGGGAt                                                                                                                                                      <  1:233654/251‑1 (MQ=255)                                                                             gtgAAAGTCTTAATTGAGATCATACGCTCTGTACTTCGGCAGTAGGAAAATATTGCCGATGATCGGGTGTTGTAGCGTTATCAACAAAAACGAAAAACCGATAATTTACTCATGCAATCGGGCATGGATCTGTTTTATAAGGCGTTTAAGGAAAAGATTTTGCCATTGAAAATGCTACGAAATATCGCATTAGTAGCGGCAGATaaa                                                                                                                                                                                                 >  1:422157/1‑207 (MQ=255)                                                                                 aaGTCTTAATTGGGATCATACGCTCTGTACTTCGGCAGTAGGAAAATATTGCCGATGATCGGGTGTTGTAGCGTTATCAACAAAAACGAAAAACCGATAATTTACTCATGCAATCGGGCATGGATCTGTTTTATAAGGCGTTTAAGGAAAAGATTTTGCCATTGAAAATGCTACGAAATATCGCATTAGTAGCGGCAGATAAAACCTCGTTGTTGAAAAAACAAGCCTTGCGTTACGCCTTGGGATTATaa                                                                                                                                                 <  1:432863/251‑1 (MQ=255)                                                                                 aaGTCTTAATTGAGATCATACGCTCTGTACTTCGGCAGTAGGAAAATATTGCCGATGATCGGGTGTTGTAGCGTTATCAACAAAAACGAAAAACCGATAATTTACTCATGCAATCGGGCATGGATCTGTTTTATAAGGCGTTTAAGGAAAAGATTTTGCCATTGAAAATGCTACGAAATATCGCATTAGTAGCGGCAGATAAAACCCCGTTGTTGAAAAAACAAGCCTTGCGTTACGCCTTGGGATTATaa                                                                                                                                                 <  1:176874/251‑1 (MQ=255)                                                                                 aaGTCTTAATTGAGATCATACGCTCTGTACTTCGGCAGTAGGAAAATATTGCCGATGATCGGGTGTTGTAGCGTTATCAACAAAAACGAAAAACCGATAATTTACTCATGCAATCGGGCATGGATCTGTTTTATAAGGCGTTTAAGGAAAAGATTTTGCCATTGAAAATGCTACGAAATATCGCATTAGTAGCGGCAGATAAAACCCCGTTGTTGAAAAAACAAGCCTTGCGTTACGCCTTGGGATTATaa                                                                                                                                                 <  1:210968/251‑1 (MQ=255)                                                                                   gTCTTAATTGAGATCATACGCTCTGTACTTCGGCAGTAGGAAAATATTGCCGATGATCGGGTGTTGTAGCGTTATCAACAAAAACGAAAAACCGATAATTTACTCATGCAATCGGGCATGGATCTGTTTTATAAGGCGTTTAAGGAAAAGATTTTGCCATTGAAAATGCTACGAAATATCGCATTAGTAGCGGCAGATAAAACCCCGTTGTTGAAAAAACAAGCCTTGCGTTACGCCTTGGGATTATaa                                                                                                                                                 <  1:508741/249‑1 (MQ=255)                                                                                   gTCTTAATTGAGATCATACGCTCTGTACTTCGGCAGTAGGAAAATATTGCCGATGATCGGGTGTTGTAGCGTTATCAACAAAAACGAAAAACCGATAATTTACTCATGCAATCGGGCATGGATCTGTTTTATAAGGCGTTTAAGGAAAAGATTTTGCCATTGAAAATGCTACGAAATATCGCATTAGTAGCGGCAGATAAAACCCCGTTGTTGAAAAAACAAGCCTTGCGTTACGCCTTGGGATTATAAt                                                                                                                                                >  1:760428/1‑250 (MQ=255)                                                                                    tCTTAATTGAGATCATACGCTCTGTACTTCGGCAGTAGGAAAATATTGCCGATGATCGGGTGTTGTAGCGTTATCAACAAAAACGAAAAACCGATAATTTACTCATGCAATCGGGCATGGATCTGTTTTATAAGGCGTTTAAGGAAAAGATTTTGCCATTGAAAATGCTACGAAATATCGCATTAGTAGCGGCAGATAAAACCCCGTTGTTGaaaaa                                                                                                                                                                                <  1:97322/217‑1 (MQ=255)                                                                                       tAATTGAGATCATACGCTCTGTACTTCGGCAGTAGGAAAATATTGCCGATGATCGGGTGTTGTAGCGTTATCAACAAAAACGAAAAACCGATAATTTACTCATGCAATCGGGCATGGATCTGTTTTATAAGGCGTTTAAGGAAAAGATTTTGCCATTGAAAATGCTACGAAATATCGCATTAGTAGCGGCAGATAAAACCCCGTTGTTGAAAAAACAAGCCTTGCGTTACGCCTTGGGATTATaa                                                                                                                                                 >  1:229645/1‑245 (MQ=255)                                                                                              gaTCATACGCTCTGTACTTCGGCAGTAGGAAAATATTGCCGATGATCGGGTGTTGTAGCGTTATCAACAAAAACGAAAAACCGATAATTTACTCATGCAATCGGGCATGGATCTGTTTTATAAGGCGTTTAAGGAAAAGATTTTGCCATTGAAAATGCTACGAAATATCGCATTAGTAGCGGCAGATAAAACCCCGTTGTTGAAAAAACAAGCCTTGCGTTACGCCTTGGGATTATaa                                                                                                                                                 <  1:207532/238‑1 (MQ=255)                                                                                                tCATACGCTCTGTACTTCGGCAGTAGGAAAATATTGCCGATGATCGGGAGTTGTAGCGTTATCAACAAAAACGAAAAACCGATAATTTACTCATGCAATCGGGCATGGATCTGTTTTATAAGGCGATTAAGGAAAAGATTTTGCCATTGAAAATGCTACGAAATATCGCATTAGTAGCGGCAGATAAAACCCCGTTGTTGAAAAAACAAGCCTTGCGTTACGCCTTGGGATTATAATCTGCCGTTGATaaa                                                                                                                                  >  1:772663/1‑251 (MQ=255)                                                                                                 cATACGCTCTGTACTTCGGCAGTAGGAAAATATTGCCGATGATCGGGTGTTGTAGCGTTATCAACAAAAACGAAAAACCGATAATTTACTCATGCAATCGGGCATGGATCTGTTTTATAAGGCGTTTAAGGAAAAGATTTTGCCATTGAAAATGCTACGAAATATCGCATTAGTAGCGGCAGATaaa                                                                                                                                                                                                 >  1:756081/1‑187 (MQ=255)                                                                                                 cATACGCTCTGTACTTCGGCAGTAGGAAAATATTGCCGATGATCGGGTGTTGTAGCGTTATCAACAAAAACGAAAAACCGATAATTTACTCATGCAATCGGGCATGGATCTGTTTTATAAGGCGTTTAAGGAAAAGATTTTGCCATTGAAAATGCTACGAAATATCGCATTAGTAGCGGCAGATAAAACCCCGTTGTTGAAAAAACAAGCCTTGCGTTACGCCTTGGGATTATAATCTGc                                                                                                                                            <  1:641959/240‑1 (MQ=255)                                                                                                 cATACGCTCTGTACTTCGGCAGTAGGAAAATATTGCCGATGATCGGGTGTTGTAGCGTTATCAACAAAAACGAAAAACCGATAATTTACTCATGCAATCGGGCATGGATCTGTTTTATAAGGCGTTTAAGGAAAAGATTTTGCCATTGAAAATGCTACGAAATATCGCATTAGTAGCGGCAGATAAAACCCCGTTGTTGAAAAAACAAGCCTTGCGTTACGCCTTGGGATTATAATCTGCCGTTGATAAAt                                                                                                                                 >  1:659544/1‑251 (MQ=255)                                                                                                      gCTCTGTACTTCGGCAGTAGGAAAATATTGCCGATGATCGGGTGTTGTAGCGTTATCAACAAAAACGAAAAACCGATAATTTACTCATGCAATCGGGCATGGATCTGTTTTATAAGGCGTTTAAGGAAAAGATTTTGCCATTGAAAATGCTACGAAat                                                                                                                                                                                                                         <  1:259176/158‑1 (MQ=255)                                                                                                      gCTCTGTACTTCGGCAGTAGGAAAATATTGCCGATGATCGGGTGTTGTAGCGTTATCAACAAAAACGAAAAACCGATAATTTACTCATGCAATCGGGCATGGATCTGTTTTATAAGGCGTTTAAGGAAAAGATTTTGCCATTGAAAATGCTACGAAATATCGCATTAGTAGCGGCAGATAAAACCCCgttgt                                                                                                                                                                                       >  1:232763/1‑192 (MQ=255)                                                                                                      gCTCTGTACTTCGGCAGTAGGAAAATATTGCCGATGATCGGGTGTTGTAGCGTTATCAACAAAAACGAAAAACCGATAATTTACTCATGCAATCGGGCATGGATCTGTTTTATAAGGCGTTTAAGGAAAAGATTTTGCCATTGAAAATGCTACGAAATATCGCATTAGTAGCGGCAGATAAAACCCCGTTGTTGAAAAAACAAGCCTTGCGTTACGCCTTGGGATTATaa                                                                                                                                                 <  1:495640/230‑1 (MQ=255)                                                                                                      gCTCTGTACTTCGGCAGTAGGAAAATATTGCCGATGATCGGGTGTTGTAGCGTTATCAACAAAAACGAAAAACCGATAATTTACTCATGCAATCGGGCATGGATCTGTTTTATAAGGCGTTTAAGGAAAAGATTTTGCCATTGAAAATGCTACGAAATATCGCATTAGTAGCGGCAGATAAAACCCCGTTGTTGAAAAAACAAGCCTTGCGTTACGCCTTGGGATTATAAt                                                                                                                                                >  1:847901/1‑231 (MQ=255)                                                                                                       ctctGTACTTCGGCAGTAGGAAAATATTGCCGATGATCGGGTGTTGTAGCGTTATCAACAAAAACGAAAAACCGATAATTTACTCATGCAATCGGGCATGGATCTGTTTTATAAGGCGTTTAAGGAAAAGATTTTGCCATTGAAAATGCTACGAAATATCGCATTAGTAGCGGCAGATAAAACCCCGTTGTTGAAAAAACAAGCCTTGCGTTACGCCTTGGGATTATAATCTGCCGTTGATAAAt                                                                                                                                 <  1:600099/245‑1 (MQ=255)                                                                                                         ctGTACTTCGGCAGTAGGAAAATATTGCCGATGATCGGGTGTTGTAGCGTTATCAACAAAAACGAAAAACCGATAATTTACTCATGCAATCGGGCATGGATCTGTTTTATAAGGCGTTTAAGGAAAAGATTTTGCCATTGAAAATGCTACGAAATATCGCATTAGTAGCGGCAGATAAAACCCCGTTGTTGAAAAAACAAGCCTTGCGTTACGCCTTGGGATTATAATCTGCCGTTGATAAATCTAAatat                                                                                                                         >  1:129577/1‑251 (MQ=255)                                                                                                         ctGTACTTCGGCAGTAGGAAAATATTGCCGATGATCGGGTGTTGTAGCGTTATCAACAAAAACGAAAAACCGATAATTTACTCATGCAATCGGGCATGGATCTGTTTTATAAGGCGTTTAAGGAAAAGATTTTGCCATTGAAAATGCTACGAAATATCGCATTAGTAGCGGCAGATAAAACCCCGTTGTTGAAAAAACAAGCCTTGCGTTACGCCTTGGGATTATAATCTGCCGTTGATAAATCTAAatat                                                                                                                         >  1:68256/1‑251 (MQ=255)                                                                                                         ctGTACTTCGGCAGTAGGAAAATATTGCCGATGATCGGGTGTTGTAGCGTTATCAACAAAAACGAAAAACCGATAATTTACTCATGCAATCGGGCATGGATCTGTTTTATAAGGCGTTTAAGGAAAAGATTTTGCCATTGAAAATGCTACGAAATATCGCATTAGTAGCGGCAGATAAAACCCCGTTGTTGAAAAAACAAGCCTTGCGTTACGCCTTGGGATTATAATCTGCCGTTGACAAATCTAAatat                                                                                                                         >  1:57973/1‑251 (MQ=255)                                                                                                           gTACTTCGGCAGTAGGAAAATATTGCCGATGATCGGGTGTTGTAGCGTTATCAACAAAAACGAAAAACCGATAATTTACTCATGCAATCGGGCATGGATCTGTTTTATAAGGCGTTTAAGGAAAAGATTTTGCCATTGAAAATGCTACGAAATATCGCATTAGTAGCGGCAGATAAAACCCCGTTGTTGAAAAAACAAGCCTTGCGTTACGCCTTGGGATTATaa                                                                                                                                                 <  1:12281/225‑1 (MQ=255)                                                                                                           gTACTTCGGCAGTAGGAAAATATTGCCGATGATCGGGTGTTGTAGCGTTATCAACAAAAACGAAAAACCGATAATTTACTCATGCAATCGGGCATGGATCTGTTTTATAAGGCGTTTAAGGAAAAGATTTTGCCATTGAAAATGCTACGAAATATCGCATTAGTAGCGGCAGATAAAACCCCGTTGTTGAAAAAACAAGCCTTGCGTTACGCCTTGGGATTATAATCTGCCGTTGATAAATCTAAATAtt                                                                                                                        >  1:150522/1‑250 (MQ=255)                                                                                                                    cAGTAGGAAAATATTGCCGATGATCGGGTGTTGTAGCGTTATCAACAAAAACGAAAAACCGATAATTTACTCATGCAATCGGGCATGGATCTGTTTTATAAGGCGTTTAAGGAAAAGATTTTGCCATTGAAAATGCTACGAAATATCGCATTAGTAGCGGCAGATAAAACCCCGTTGTTGAAAAAACAAGCCTTGCGTTACGCCTTGGGATTATAATCTGCCGTTGATAAATCTAAATATTCGATTATTg                                                                                                               <  1:580122/250‑1 (MQ=255)                                                                                                                      gTAGGAAAATATTGCCGATGATCGGGTGTTGTAGCGTTATCAACAAAAACGAAAAACCGATAATTTACTCATGCAATCGGGCATGGATCTGTTTTATAAGGCGTTTAAGGAAAAGATTTTGCCATTGAAAATGCTACGAAATATCGCATTAGTAGCGGCAGATAAAACCCCGTTGTTGAAAAAACAAGCCTTGCGTTACGCCTTGGGATTATAATCTGCCGTTGATAAATCTAAATATTCGATTATTGCCa                                                                                                            >  1:252389/1‑251 (MQ=255)                                                                                                                       tAGGAAAATATTGCCGATGATCGGGTGTTGTAGCGTTATCAACAAAAACGAAAAACCGATAATTTACTCATGCAATCGGGCATGGATCTGTTTTATAAGGCGTTTAAGGAAAAGATTTTGCCATTGAAAATGCTACGAAATATCGCATTAGTAGCGGCAGATAAAACCCCGTTGTTGAAAAAACAAGCCTTGCGTTACGCCTTGGGATTATAATCTGCCGTTGATAAATCTAAATATTCGATTATTGcc                                                                                                             <  1:617866/249‑1 (MQ=255)                                                                                                                       tAGGAAAATATTGCCGATGATCGGGTGTTGTAGCGTTATCAACAAAAACGAAAAACCGATAATTTACTCATGCAATCGGGCATGGATCTGTTTTATAAGGCGTTTAAGGAAAAGATTTTGCCATTGAAAATGCTACGAAATATCGCATTAGTAGCGGCAGATAAAACCCCGTTGTTGAAAAAACAAGCCTTGCGTTACGCCTTGGGATTATAATCTGCCGTTGATAAATCTAAATATTCGATTATTGcc                                                                                                             <  1:205505/249‑1 (MQ=255)                                                                                                                       tAGGAAAATATTGCCGATGATCGGGTGTTGTAGCGTTATCAACAAAAACGAAAAACCGATAATTTACTCATGCAATCGGGCATGGATCTGTTTTATAAGGCGTTTAAGGAAAAGATTTTGCCATTGAAAATGCTACGAAATATCGCATTAGTAGCGGCAGATAAAACCCCGTTGTTGAAAAAACAAGCCTTGCGTTACGCCTTGGGATTATAATCTGCCGTTGATAAATCTAAATATTCGATTATTGCCaa                                                                                                           >  1:113468/1‑251 (MQ=255)                                                                                                                        aGGAAAATATTGCCGATGATCGGGTGTTGTAGCGTTATCAACAAAAACGAAAAACCGATAATTTACTCATGCAATCGGGCATGGATCTGTTTTATAAGGCGTTTAAGGAAAAGATTTTGCCATTGAAAATGCTACGAAATATCGCATTAGTAGCGGCAGATAAAACCCCGTTGTTGAAAAAACAAGCCTTGCGTTACGCCTTGGGATTATAATCTGCCGTTGATAAATCTAAATATTCGATTATTGCCAat                                                                                                          >  1:711987/1‑251 (MQ=255)                                                                                                                               tatTGCCGATGATCGGGTGTTGTAGCGTTATCAACAAAAACGAAAAACCGATAATTTACTCATGCAATCGGGCATGGATCTGTTTTATAAGGCGTTTAAGGAAAAGATTTTGCCATTGAAAATGCTACGAAATATCGCATTAGTAGCGGCAGATAAAACCCCGTTGTTGAAAAAACAAGCCTTGCGTTACGCCTTGGGATTATAATCTGCCGTTGATAAATCTAAATATTCGATTATTGCCAATATAAAAt                                                                                                   <  1:729121/251‑1 (MQ=255)                                                                                                                                   gCCGATGATCGGGTGTTGTAGCGTTATCAACAAAAACGAAAAACCGATAATTTACTCATGCAATCGGGCATGGATCTGTTTTATAAGGCGTTTAAGGAAAAGATTTTGCCATTGAAAATGCTACGAAATATCGCATTAGTAGCGGCAGATAAAACCCCGTTGTTGAAAAAAc                                                                                                                                                                              >  1:737950/1‑172 (MQ=255)                                                                                                                                   gCCGATGATCGGGTGTTGTAGCGTTATCAACAAAAACGAAAAACCGATAATTTACTCATGCAATCGGGCATGGATCTGTTTTATAAGGCGTTTAAGGAAAAGATTTTGCCATTGAAAATGCTACGAAATATCGCATTAGTAGCGGCAGATAAAACCCCGTTGTTGAAAAAACAAGCCTTGCGTTACGCCTTGGGATTATAATCTGCCGTTGATAAATCTAAATATTCGATTATTGCCAATATAAAATCttt                                                                                               >  1:697500/1‑251 (MQ=255)                                                                                                                                       atgatCGGGTGTTGTAGCGTTATCAACAAAAACGAAAAACCGATAATTTACTCATGCAATCGGGCATGGATCTGTTTTATAAGGCGTTTAAGGAAAAGATTTTGCCATTGAAAATGCTACGAAATATCGCATTAGTAGCGGCAGATAAAACCCCGTTGTTGAAAAAACAAGCCTTGCGTTACGCCTTGGGATTATAATCTGCCGTTGGTAAATc                                                                                                                                >  1:702213/1‑214 (MQ=255)                                                                                                                                          atCGGGTGTTGTAGCGTTATCAACAAAAACGAAAAACCGATAATTTACTCATGCAATCGGGCATGGATCTGTTTTATAAGGCGTTTAAGGAAAAGATTTTGCCATTGAAAATGCTACGAAATATCGCATTAGTAGCGGCAGATAAAACCCCGTTGTTGAAAAAACAAGCCTTGCGTTACGCCTTGGGATTATAATCTGCCGTTGATAAATCTAAATATTCGATTATTGCCAATATAAAATCTTTTAATTTa                                                                                        >  1:509457/1‑251 (MQ=255)                                                                                                                                              ggTGTTGTAGCGTTATCAACAAAAACGAAAAACCGATAATTTACTCATGCAATCGGGCATGGATCTGTTTTATAAGGCGTTTAAGGAAAAGATTTTGCCATTGAAAATGCTACGAAATATCGCATTAGTAGCGGCAGATAAAACCCCGTTGTTGAAAAAACAAGCCTTGCGt                                                                                                                                                                   >  1:561916/1‑172 (MQ=255)                                                                                                                                              ggTGTTGTAGCGTTATCAACAAAAACGAAAAACCGATAATTTACTCATGCAATCGGGCATGGATCTGTTTTATAAGGCGTTTAAGGAAAAGATTTTGCCATTGAAAATGCTACGAAATATCGCATTAGTAGCGGCAGATAAAACCCCGTTGTTGAAAAAACAAGCCTTGCGTTACGCCTTGGGATTATAAt                                                                                                                                                >  1:342309/1‑191 (MQ=255)                                                                                                                                               gtgtTGTAGCGTTATCAACAAAAACGAAAAACCGATAATTTACTCATGCAATCGGGCATGGATCTGTTTTATAAGGCGTTTAAGGAAAAGATTTTGCCATTGAAAATGCTACGAAATATCGCATTAGTAGCGGCAGATAAAACCCCGTTGTTGAAAAAACAAGCCTTGCGTTACGCCTTGGGATTATAATCTGCCGTTGATAAATCTAAat                                                                                                                           >  1:666231/1‑211 (MQ=255)                                                                                                                                               gtgtTGTAGCGTTATCAACAAAAACGAAAAACCGATAATTTACTCATGCAATCGGGCATGGATCTGTTTTATAAGGCGTTTAAGGAAAAGATTTTGCCATTGAAAATGCTACGAAATATCGCATTAGTAGCGGCAGATAAAACCCCGTTGTTGAAAAAACAAGCCTTGCGTTACGCCTTGGGATTATAATCTGCCGTTGATAAATCTAAATATTCGATTATTGCCAATATAAAATCTTTTAATTTACATg                                                                                    >  1:312252/1‑250 (MQ=255)                                                                                                                                                 gttgtAGCGTTATCAACAAAAACGAAAAACCGATAATTTACTCATGCAATCGGGCATGGATCTGTTTTATAAGGCGTTTAAGGAAAAGATTTTGCCATTGAAAATGCTACGAAATATCGCATTAGTAGCGGCAGATAAAACCCCGTTGTTGAAAAAACAAGCCTTGCGTTACGCCTTGGGATTATAATCTGCCGTTGATAAATCTAAATATTCGATTATTGCCAATATAAAAt                                                                                                   <  1:58103/233‑1 (MQ=255)                                                                                                                                                         gTTATCAACAAAAACGAAAAACCGATAATTTACTCATGCAATCGGGCATGGATCTGTTTTATAAGGCGTTTAAGGAAAAGATTTTGCCATTGAAAATGCTACGAAATATCGCATTAGTAGCGGCAGATAAAACCCCGTTGTTGAAAAAACAAGCCTTGCGTTACGCCTTGGGATTATAATCTGCCGTTGATAAATCTAAATATTCGATTATTGCCAATATAAAATCTTTTAATTTACATGCTCAATGTTTa                                                                         >  1:270171/1‑251 (MQ=255)                                                                                                                                                           tATCAACAAAAACGAAAAACCGATAATTTACTCATGCAATCGGGCATGGATCTGTTTTATAAGGCGTTTAAGGAAAAGATTTTGCCATTGAAAATGCTACGAAATATCGCATTAGTAGCGGCAGATAAAACCCCGTTGTTGAAAAAACAAGCCTTGCGTTACGCCTTGGGATTATAATCTGCCGTTGATAAATCTAAATATTCGATTATTGCCAATATAAAATCTTTTAATTTACATGCTCAATGTTTAAg                                                                       <  1:869632/251‑1 (MQ=255)                                                                                                                                                              caacaaAAACGAAAAACCGATAATTTACTCATGCAATCGGGCATGGATCTGTTTTATAAGGCGTTTAAGGAAAAGATTTTGCCATTGAAAATGCTACGAAATATCGCATTAGTAGCGGCAGATAAAACCCCGTTGTTGAAAAAACAAGCCTTGCGTTACGCCTTGGGATTATAATCTGCCGTTGATAAATCTAAATATTCGATTATTGCCAATATAAAATCTTTTAATTTACATGCTCaa                                                                               <  1:777113/240‑1 (MQ=255)                                                                                                                                                                      aCGAAAAACCGATAATTTACTCATGCAATCGGGCATGGATCTGTTTTATAAGGCGTTTAAGGAAAAGATTTTGCCATTGAAAATGCTACGAAATATCGCATTAGTAGCGGCAGATAAAACCCCGTTGTTGAAAAAACAAGCCTTGCGTTACGCCTTGGGATTATAATCTGCCGTTGATAAATCTAAATATTCGATTATTGCCAATATAAAATCTTTTAATTTACATGCTCAATGTTTAAGTTATATACGat                                                            <  1:164570/251‑1 (MQ=255)                                                                                                                                                                             aCCGATAATTTACTCATGCAATCGGGCATGGATCTGTTTTATAAGGCGTTTAAGGAAAAGATTTTGCCATTGAAAATGCTACGAAATATCGCATTAGTAGCGGCAGATAAAACCCCGTTGTTGAAAAAACAAGCCTTGCGTTACGCCTTGGGATTATAATCTGCCGTTGATAAATCTAAATATTCGATTATTGCCAATATAAAATCTTTTAATTTACATGCTCAATGTTTAAGTTATATACGATATaaa                                                       >  1:742563/1‑249 (MQ=255)                                                                                                                                                                              ccGATAATTTACTCATGCAATCGGGCATGGATCTGTTTTATAAGGCGTTTAAGGAAAAGATTTTGCCATTGAAAATGCTACGAAATATCGCATTAGTAGCGGCAGATAAAACCCCGTTGTTGAAAAAACAAGCCTTGCGTTACGCCTTGGGATTATAATCTGCCGTTGATAAATCTAAATATTCGATTATTGCCAATATAAAATCTTTTAATTTACa                                                                                      >  1:473208/1‑217 (MQ=255)                                                                                                                                                                                   taTTTACTCATGCAATCGGGCATGGATCTGTTTTATAAGGCGTTTAAGGAAAAGATTTTGCCATTGAAAATGCTACGAAATATCGCATTAGTAGCGGCAGATAAAACCCCGTTGTTGAAAAAACAAGCCTTGCGTTACGCCTTGGGATTATAATCTGCCGTTGATAAATCTAAATATTCGATTATTGCCAATATAAAATCTTTTAATTTACATGCTCAATGTTTAAGt                                                                      <  1:712461/227‑1 (MQ=255)                                                                                                                                                                                      ttACTCATGCAATCGGGCATGGATCTGTTTTATAAGGCGTTTAAGGAAAAGATTTTGCCATTGAAAATGCTACGAAATATCGCATTAGTAGCGGCAGATAAAACCCCGTTGTTGAAAAAACAAGCCTTGCGTTACGCCTTGGGATTATaa                                                                                                                                                 >  1:187580/1‑150 (MQ=255)                                                                                                                                                                                      ttACTCATGCAATCGGGCATGGATCTGTTTTATAAGGCGTTTAAGGAAAAGATTTTGCCATTGAAAATGCTACGAAATATCGCATTAGTAGCGGCAGATAAAACCCCGTTGTTGAAAAAACAAGCCTTGCGTTACGCCTTGGGATTATAATCTGCCGTTGATAAATCTAAATATTCGATTATTGCCAATATAAAATCTTTTAATTTACATGCTCAATGTTTAAGTTATATACGATATAAACTAATCATTcc                                            <  1:499552/251‑1 (MQ=255)                                                                                                                                                                                           cATGCAATCGGGCATGGATCTGTTTTATAAGGCGTTTAAGGAAAAGATTTTGCCATTGAAAATGCTACGAAATATCGCATTAGTAGCGGCAGATAAAACCCCGTTGTTGAAAAAACAAGCCTTGCGTTACGCCTTGGGATTATAATCTGCCGTTGATAAATCTAAATATTCGATTATTGCCAATATAAAAt                                                                                                   <  1:557343/191‑1 (MQ=255)                                                                                                                                                                                           cATGCAATCGGGCATGGATCTGTTTTATAAGGCGTTTAAGGAAAAGATTTTGCCATTGAAAATGCTACGAAATATCGCATTAGTAGCGGCAGATAAAACCCCGTTGTTGAAAAAACAAGCCTTGCGTTACGCCTTGGGATTATAATCTGCCGTTGATAAATCTAAATATTCGATTATTGCCAATATAAAATCTTTTAATTTACATGCTCAATGTTTAAGTTATATACGATATAAACTAATCATTCCAATaa                                       >  1:748368/1‑251 (MQ=255)                                                                                                                                                                                            aTGCAATCGGGCATGGATCTGTTTTATAAGGCGTTTAAGGAAAAGATTTTGCCATTGAAAATGCTACGAAATATCGCATTAGTAGCGGCAGATAAAACCCCGTTGTTGAAAAAACAAGCCTTGCGTTACGCCTTGGGATTATAATCTGCCGTTGATAAATCTAAATATTCGATTATTGCCAATATaaa                                                                                                     <  1:291890/188‑1 (MQ=255)                                                                                                                                                                                             tGCAATCGGGCATGGATCTGTTTTATAAGGCGTTTAAGGAAAAGATTTTGCCATTGAAAATGCTACGAAATATCGCATTAGTAGCGGCAGATAAAACCCCGTTGTTGAAAAAACAAGCCTTGCGTTACGCCTTGGGATTATAATCTGCCGTTGATAAATCTAAATATTCGATTATTGCCAATATAAAATCTTTTAATTTACATGCTCAATGTTTAAGTTATATACGATATAAACTAATCATTCCAATAAAg                                     <  1:337713/251‑1 (MQ=255)                                                                                                                                                                                               cAATCGGGCATGGATCTGTTTTATAAGGCGTTTAAGGAAAAGATTTTGCCATTGAAAATGCTACGAAATATCGCATTAGTAGCGGCAGATAAAACCCCGTTGTTGAAAAAACAAGCCTTGCGTTACGCCTTGGGATTATAATCTGCCGTTGATAAATCTAAATATTCGATTATTGCCAATATAAAATCTTTTAATTTACATGCTCAATGTTTAAGTTATATACGATATAAACTAATCATTCCAATAAAGa                                    <  1:170305/250‑1 (MQ=255)                                                                                                                                                                                                         tGGATCTGTTTTATAAGGCGTTTAAGGAAAAGATTTTGCCATTGAAAATGCTACGAAATATCGCATTAGTAGCGGCAGATAAAACCCCGTTGTTGAAAAAACAAGCCTTGCGTTACGCCTTGGGATTATAATc                                                                                                                                               >  1:287677/1‑133 (MQ=255)                                                                                                                                                                                                           gATCTGTTTTATAAGGCGTTTAAGGAAAAGATTTTGCCATTGAAAATGCTACGAAATATCGCATTAGTAGCGGCAGATAAAACCCCGTTGTTGAAAAAACAAGCCTTGCGTTACGCCTTGGGATTATAATCTGCCGTTGATAAATCTAAATATTCGATTATTGCCAATATAAAATCTTTTAATTTACATGCTCAATGTTTAAGTTATATAc                                                               <  1:562413/211‑1 (MQ=255)                                                                                                                                                                                                           gATCTGTTTTATAAGGCGTTTAAGGAAAAGATTTTGCCATTGAAAATGCTACGAAATATCGCATTAGTAGCGGCAGATAAAACCCCGTTGTTGAAAAAACAAGCCTTGCGTTACGCCTTGGGATTATAATCTGCCGTTGATAAATCTAAATATTCGATTATTGCCAATATAAAATCTTTTAATTTACATGCTCAATGTTTAAGTTATATAc                                                               >  1:884932/1‑211 (MQ=255)                                                                                                                                                                                                                gTTTTATAAGGCGTTTAAGGAAAAGATTTTGCCATTGAAAATGCTACGAAATATCGCATTAGTAGCGGCAGATAAAACCCCGTTGTTGAAAAAACAAGCCTTGCGTTACGCCTTGGGATTATaa                                                                                                                                                 >  1:467325/1‑124 (MQ=255)                                                                                                                                                                                                                gTTTTATAAGGCGTTTAAGGAAAAGATTTTGCCATTGAAAATGCTACGAAATATCGCATTAGTAGCGGCAGATAAAACCCCGTTGTTGAAAAAACAAGCCTTGCGTTACGCCTTGGGATTATAATCTGCCGTTGATAAATCTAAATATTCGATTATTGCCAATATAAAATCTTTTAATTTACATGCTCAATGTTTAAGTTATATACGATATAAACTAATc                                                 <  1:589392/220‑1 (MQ=255)                                                                                                                                                                                                                gTTTTATAAGGCGTTTAAGGAAAAGATTTTGCCATTGAAAATGCTACGAAATATCGCATTAGTAGCGGCAGATAAAACCCCGTTGTTGAAAAAACAAGCCTTGCGTTACGCCTTGGGATTATAATCTGCCGTTGATAAATCTAAATATTCGATTATTGCCAATATAAAATCTTTTAATTTACATGCTCAATGTTTAAGTTATATACGATATAAACTAATCATTCCAATAAAGAACCGCACTCTGAAGCTCa                  >  1:727171/1‑251 (MQ=255)                                                                                                                                                                                                                gTTTTATAAGGCGTTTAAGGAAAAGATTTTGCCATTGAAAATGCTACGAAATATCGCATTAGTAGCGGCAGATAAAACCCCGTTGTTGAAAAAACAAGCCTTGCGTTACGCCTTGGGATTATAATCTGCCGTTGATAAATCTAAATATTCGATTATTGCCAATATAAAATCTTTTAATTTACATGCTCAATGTTTAAGTTATATACGATATAAACTAATCATTCCAATAAAGAACCGCACTCTGAAGCTCa                  >  1:818157/1‑251 (MQ=255)                                                                                                                                                                                                                gTTTTATAAGGCGTTTAAGGAAAAGATTTTGCCATTGAAAATGCTACGAAATATCGCATTAGTAGCGGCAGATAAAACCCCGTTGTTGAAAAAACAAGCCTTGCGTTACGCCTTGGGATTATAATCTGCCGTTGATAAATCTAAATATTCGATTATTGCCAATATAAAATCTTTTAATTTACATGCTCAATGTTTAAGTTATATACGATATAAACTAATCATTCCAATAAAGAACCGCACTCTGAAGCTCa                  >  1:484574/1‑251 (MQ=255)                                                                                                                                                                                                                  tttATAAGGCGTTTAAGGAAAAGATTTTGCCATTGAAAATGCTACGAAATATCGCATTAGTAGCGGCAGATAAAACCCCGTTGTTGAAAAAACAAGCCTTGCGTTACGCCTTGGGATTATAATCTGCCGTTGATAAATCTAAATATTCGATTATTGCCAATATAAAAt                                                                                                   >  1:288259/1‑168 (MQ=255)                                                                                                                                                                                                                  tttATAAGGCGTTTAAGGAAAAGATTTTGCCATTGAAAATGCTACGAAATATCGCATTAGTAGCGGCAGATAAAACCCCGTTGTTGAAAAAACAAGCCTTGCGTTACGCCTTGGGATTATAATCTGCCGTTGATAAATCTAAATATTCGATTATTGCCAATATAAAATCTTTTAATTTACATGCTCAATGTTTAAGTTATATACGATATAAACTAATCATTCCAATAAAGAAc                                  >  1:645754/1‑233 (MQ=255)                                                                                                                                                                                                                           cGTTTAAGGAAAAGATTTTGCCATTGAAAATGCTACGAAATATCGCATTAGTAGCGGCAGATAAAACCCCGTTGTTGAAAAAACAAGCCTTGCGTTACGCCTTGGGATTATAATCTGCCGTTGATAAATCTAAATATTCGATTATTGCCAATATAAAATCTTTTAATTTACATGCTCAATGTTTAAGTTATATACGATATAAACTAATCATTCCAATAAAGAACCGCACTCTGAAGCTCAAAGTGCGGTCa       <  1:832285/251‑1 (MQ=255)                                                                                                                                                                                                                                aaGGAAAAGATTTTGCCATTGAAAATGCTACGAAATATCGCATTAGTAGCGGCAGATAAAACCCCGTTGTTGAAAAAACAAGCCTTGCGTTACGCCTTGGGATTATAATCTGCCGTTGATAAATCTAAATATTCGATTATTGCCAATATAAAATCTTTTAATTTACATGCTCAATGTTTAAGTTATATACGATATAAACTAATCATTCCAATAAAGAACCGCACTCTGAAGCTCAAAGTGCGGTCATTTAt  <  1:681341/251‑1 (MQ=255)                                                                                                                                                                                                                                   gAAAAGATTTTGCCATTGAAAATGCTACGAAATATCGCATTAGTAGCGGCAGATAAAACCCCGTTGTTGAAAAAACAAGCCTTGCGTTACGCCTTGGGATTATAATCTGCCGTTGATAAATCTAAatat                                                                                                                         <  1:309569/129‑1 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                             TGACGGGAATTTTAAAAGATATTATTGTTGTCAGCGGAGGCGTGATTGGTGCGGCCTCGCCTTCGGTTTTAAAGATGTGAAAGTCTTAATTGAGATCATACGCTCTGTACTTCGGCAGTAGGAAAATATTGCCGATGATCGGGTGTTGTAGCGTTATCAACAAAAACGAAAAACCGATAATTTACTCATGCAATCGGGCATGGATCTGTTTTATAAGGCGTTTAAGGAAAAGATTTTACCATTGAAAATGCTACGAAATATCGCATTAGTAGCGGCAGATAAAACCCCGTTGTTGAAAAAACAAGCCTTGCGTTACGCCTTGGGATTATAATCTGCCGTTGATAAATCTAAATATTCGATTATTGCCAATATAAAATCTTTTAATTTACATGCTCAATGTTTAAGTTATATACGATATAAACTAATCATTCCAATAAAGAACCGCACTCTGAAGCTCAAAGTGCGGTCATTTAT  >  1/1865379‑1865852

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑