Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,855,258	A→G	intergenic (‑19595/+28371)	unknown ← / ← unknown	tRNA‑Lys‑TTT/tRNA‑Ser‑TGA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,855,258	0	A	G	100.0%	191.1 / NA	67	intergenic (‑19595/+28371)	unknown/unknown	tRNA‑Lys‑TTT/tRNA‑Ser‑TGA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (33/34);  total (33/34)

GTTCTCTTTGCGTAAAAAATAAAGCATAGAAATTATCGCAGTAAACAATAACGGTACATCACAGGTTAAGGCGCCCTCACCGTAAGAATCAAATACATCGGCAAGGAAATAACCGGCAAGCAGATTGTAGCCCATAAAGAAGAAAATCCCGATGATAATCGACATATGACTTTCCGCGTGGGGATTGCCAAAATGCAACCCCAGCGCAATAAACAACACAATAACGTTTAAAATCAGCAAACCAGTTTTCGGTTCAGACACATTTAACGTTAAAAAAAGCCAAATTAATGCAGTCACAATACCGATAAAAATGGCAAAAATTAATAAAATGGACAGTGCCCAAGAAATGTTCTTAAGGTGCGGATAATGCTGCTTATACCAACCATGCAACAACATAATGGCAAAGATCGTAATAATAAAAAGCAACAGGCCTGCCAATACGGCAAAATCACCGGAATTTACATTGGAAAA  >  1/1855015‑1855485                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                    gTTCTCTTTGCGTAAAAAATAAAGCATAGAAATTATCGCAGTAAACAATAACGGTACATCACAGGTTAAGGCGCCCTCACCGTAAGAATCAAATACATCGGCAAGGAAATAACCGGCAAGCAGATTGTAGCCCATAAAGAAGAAAATCCCGATGATAATCGACATATGACTTTCCGCGTGGGGATTGCCAAAATGCAACCCCAGCGCAATAAACAACACAATAACGTTTAAAATCAGCAAACCGGTTTTCg                                                                                                                                                                                                                              >  1:529704/1‑251 (MQ=255)  ttCTCTTTGCGTAAAAAATAAAGCATAGAAATTATCGCAGTAAACAATAACGGTACATCACAGGTTAAGGCGCCCTCACCGTAAGAATCAAATACATCGGCAAGGAAATAACCGGCAAGCAGATTGTAGCCCATAAAGAAGAAAATCCCGATGATAATCGACATATGACTTTCCGCGTGGGGATTGCCAAAATGCAACCCCAGCGCAATAAACAACACAATAACGTTTAAAATCAGCAAACCGGtttt                                                                                                                                                                                                                                <  1:110547/248‑1 (MQ=255)    ctctTTGCGTAAAAAATAAAGCATAGAAATTATCGCAGTAAACAATAACGGTACATCACAGGTTAAGGCGCCCTCACCGTAAGAATCAAATACATCGGCAAGGAAATAACCGGCAAGCAGATTGTAGCCCATAAAGAAGAAAATCCCGATGATAATCGACATATGACTTTCCGCGTGGGGATTGCCAAAATGCAACCCCAGCGCAATAAACAACACAATAACGTTTAAAATCAGCAAACCGGTTTTCGGtt                                                                                                                                                                                                                           >  1:699713/1‑251 (MQ=255)           cGTAAAAAATAAAGCATAGAAATTATCGCAGTAAACAATAACGGTACATCACAGGTTAAGGCGCCCTCACCGTAAGAATCAAATACATCGGCAAGGAAATAACCGGCAAGCAGATTGTAGCCCATAAAGAAGAAAATCCCGATGATAATCGACATATGACTTTCCGCGTGGGGATTGCCAAAATGCAACCCCAGCGCAATAAACAACACAATAACGTTTAAAATCAGCAAACCGGTTTTCGGTTCAGaca                                                                                                                                                                                                                     <  1:304613/250‑1 (MQ=255)           cGTAAAAAATAAAGCATAGAAATTATCGCAGTAAACAATAACGGTACATCACAGGTTAAGGCGCCCTCACCGTAAGAATCAAATACATCGGCAAGGAAATAACCGGCAAGCAGATTGTAGCCCATAAAGAAGAAAATCCCGATGATAATCGACATATGACTTTCCGCGTGGGGATTGCCAAAATGCAACCCCAGCGCAATAAACAACACAATAACGTTTAAAATCAGCAAACCGGTTTTCGGTTCAGaca                                                                                                                                                                                                                     <  1:755583/250‑1 (MQ=255)                   aTAAAGCATAGAAATTATCGCAGTAAACAATAACGGTACATCACAGGTTAAGGCGCCCTCACCGTAAGAATCAAATACATCGGCAAGGAAATAACCGGCAAGCAGATTGTAGCCCATAAAGAAGAAAATCCCGATGATAATCGACATATGACTTTCCGCGTGGGGATTGCCAAAATGCAACCCCAGCGCAATAAACAACACAATAACGTTTAAAATCAGCAAACCGGTTTTCGGTTCAGACACATTTAACg                                                                                                                                                                                                            <  1:601779/251‑1 (MQ=255)                   aTAAAGCATAGAAATTATCGCAGTAAACAATAACGGTACATCACAGGTTAAGGCGCCCTCACCGTAAGAATCAAATACATCGGCAAGGAAATAACCGGCAAGCAGATTGTAGCCCATAAAGAAGAAAATCCCGATGATAATCGACATATGACTTTCCGCGTGGGGATTGCCAAAATGCAACCCCAGCGCAATAAACAACACAATAACGTTTAAAATCAGCAAACCGGTTTTCGGTTCAGACACATTTAACg                                                                                                                                                                                                            <  1:141415/251‑1 (MQ=255)                        gCATAGAAATTATCGCAGTAAACAATAACGGTACATCACAGGTTAAGGCGCCCTCACCGTAAGAATCAAATACATCGGCAAGGAAATAACCGGCAAGCAGATTGTAGCCCATAAAGAAGAAAATCCCGATGATAATCGACATATGACTTTCCGCGTGGGGATTGCCAAAATGCAACCCCAGCGCAATAAACAACACAATAACGTTTAAAATCAGCAAACCGGTTTTCGGTTCAGACACATTTAACGTTaaa                                                                                                                                                                                                       >  1:266556/1‑251 (MQ=255)                             gAAATTATCGCAGTAAACAATAACGGTACATCACAGGTTAAGGCGCCCTCACCGTAAGAATCAAATACATCGGCAAGGAAATAACCGGCAAGCAGATTGTAGCCCATAAAGAAGAAAATCCCGATGATAATCGACATATGACTTTCCGCGTGGGGATTGCCAAAATGCAACCCCAGCGCAATAAACAACACAATAACGTTTAAAATCAGCAAACCGGTTTTCGGTTCAGACACATTTAACGTTAAAAAAAg                                                                                                                                                                                                  >  1:712904/1‑251 (MQ=255)                                      gCAGTAAACAATAACGGTACATCACAGGTTAAGGCGCCCTCACCGTAAGAATCAAATACATCGGCAAGGAAATAACCGGCAAGCAGATTGTAGCCCATAAAGAAGAAAATCCCGATGATAATCGACATATGACTTTCCGCGTGGGGATTGCCAAAATGCAACCCCAGCGCAATAAACAACACAATAACGTTTAAAATCAGCAAACCGGTTTTCGGTTCAGACACATTTAACGTTAAAAAAAGCCAAATTaa                                                                                                                                                                                         >  1:761291/1‑251 (MQ=255)                                          tAAACAATAACGGTACATCACAGGTTAAGGCGCCCTCACCGTAAGAATCAAATACATCGGCAAGGAAATAACCGGCAAGCAGATTGTAGCCCATAAAGAAGAAAATCCCGATGATAATCGACATATGACTTTCCGCGTGGGGATTGCCAAAATGCAACCCCAGCGCAATAAACAACACAATAACGTTTAAAATCAGCAAACCGGTTTTCGGTTCAGACACATTTAACGTTAAAAAAAGCCAAATTAATGc                                                                                                                                                                                      <  1:91741/250‑1 (MQ=255)                                             aCAATAACGGTACATCACAGGTTAAGGCGCCCTCACCGTAAGAATCAAATACATCGGCAAGGAAATAACCGGCAAGCAGATTGTAGCCCATAAAGAAGAAAATCCCGATGATAATCGACATATGACTTTCCGCGTGGGGATTGCCAAAATGCAACCCCAGCGCAATAAACAACACAATAACGTTTAAAATCAGCAAACCGGTTTTCGGTTCAGACACATTTAACGTTAAAAAAAGCCAAATTAATGCAGt                                                                                                                                                                                   >  1:488631/1‑250 (MQ=255)                                              cAATAACGGTACATCACAGGTTAAGGCGCCCTCACCGTAAGAATCAAATACATCGGCAAGGAAATAACCGGCAAGCAGATTGTAGCCCATAAAGAAGAAAATCCCGATGATAATCGACATATGACTTTCCGCGTGGGGATTGCCAAAATGCAACCCCAGCGCAATAAACAACACAATAACGTTTAAAATCAGCAAACCGGTTTTCGGTTCAGACACATTTAACGTTAAAAAAAGCCAAATTAATGCAGTca                                                                                                                                                                                 >  1:144449/1‑251 (MQ=255)                                                aTAACGGTACATCACAGGTTAAGGCGCCCTCACCGTAAGAATCAAATACATCGGCAAGGAAATAACCGGCAAGCAGATTGTAGCCCATAAAGAAGAAAATCCCGATGATAATCGACATATGACTTTCCGCGTGGGGATTGCCAAAATGCAACCCCAGCGCAATAAACAACACAATAACGTTTAAAATCAGCAAACCGGTTTTCGGTTCAGACACATTTAACGTTAAAAAAAGCCTAATTAATGCAGTcaca                                                                                                                                                                               >  1:49633/1‑251 (MQ=255)                                                    cGGTACATCACAGGTTAAGGCGCCCTCACCGTAAGAATCAAATACATCGGCAAGGAAATAACCGGCAAGCAGATTGTAGCCCATAAAGAAGAAAATCCCGATGATAATCGACATATGACTTTCCGCGTGGGGATTGCCAAAATGCAACCCCAGCGCAATAAACAACACAATAACGTTTAAAATCAGCAAACCGGTTTTCGGTTCAGACACATTTAACGt                                                                                                                                                                                                           <  1:849111/219‑1 (MQ=255)                                                       tACATCACAGGTTAAGGCGCCCTCACCGTAAGAATCAAATACATCGGCAAGGAAATAACCGGCAAGCAGATTGTAGCCCATAAAGAAGAAAATCCCGATGATAATCGACATATGACTTTCCGCGTGGGGATTGCCAAAATGCAACCCCAGCGCAATAAACAACACAATAACGTTTAAAATCAGCAAACCGGTTTTCGGTTCAGACACA‑‑TAACGTTAAAAAAAGCCAAATTAATGCAGTCACAATACCGATa                                                                                                                                                                      <  1:195913/251‑1 (MQ=255)                                                        aCATCACAGGTTAAGGCGCCCTCACCGTAAGAATCAAATACATCGGCAAGGAAATAACCGGCAAGCAGATTGTAGCCCATAAAGAAGAAAATCCCGATGATAATCGACATATGACTTTCCGCGTGGGGATTGCCAAAATGCAACCCCAGCGCAATAAACAACACAATAACGTTTAAAATCAGCAAACCGGTTTTCGGTTCAGACACATTTAACGTTAAAAAAAGCCAAATTAATGCAGTCACAATAc                                                                                                                                                                           <  1:652803/247‑1 (MQ=255)                                                           tCACAGGTTAAGGCGCCCTCACCGTAAGAATCAAATACATCGGCAAGGAAATAACCGGCAAGCAGATTGTAGCCCATAAAGAAGAAAATCCCGATGATAATCGACATATGACTTTCCGCGTGGGGATTGCCAAAATGCAACCCCAGCGCAATAAACAACACAATAACGTTTAAAATCAGCAAACCGGTTTTCGGTTCAGACACATTTAACGTTAAAAAAAGCCAAATTAATGCAGTCACAATACCGATaaa                                                                                                                                                                    >  1:308060/1‑251 (MQ=255)                                                                ggTTAAGGCGCCCTCACCGTAAGAATCAAATACATCGGCAAGGAAATAACCGGCAAGCAGATTGTAGCCCATAAAGAAGGAAATCCCGATGATAATCGACATATGACTTTCCGCGTGGGGATTGCCAAAATGCAACCCCAGCGCAATAAACAACACAATAACGTTTAAAATCAGCAAACCGGTTTTCGGTTCAGACACATTTAACGTTAAAAAAAGCCAAATTAATGCAGTCACAATACCGATAAAAATgg                                                                                                                                                               >  1:50077/1‑251 (MQ=255)                                                                    aaGGCGCCCTCACCGTAAGAATCAAATACATCGGCAAGGAAATAACCGGCAAGCAGATTGTAGCCCATAAAGAAGAAAATCCCGATGATAATCGACATATGACTTTCCGCGTGGGGATTGCCAAAATGCAACCCCAGCGCAATAAACAACACAATAACGTTTAAAATCAGCAAACCGGTTTTCGGTTCAGACACATTTAACGTTAAAAAAAGCCAAATTAATGCAGTCACAATACCGATAAAAATGGCaaa                                                                                                                                                           >  1:763010/1‑251 (MQ=255)                                                                            cTCACCGTAAGAATCAAATACATCGGCAAGGAAATAACCGGCAAGCAGATTGTAGCCCATAAAGAAGAAAATCCCGATGATAATCGACATATGACTTTCCGCGTGGGGATTGCCAAAATGCAACCCCAGCGCAATAAACAACACAATAACGTTTAAAATCAGCAAACCGGTTTTCGGTTCAGACACATTTAACGTTAAAAAAAGCCAAATTAATGCAGTCACAATACCGATAAAAATGGCAAAAATtaata                                                                                                                                                   <  1:883998/251‑1 (MQ=255)                                                                              cACCGTAAGAATCAAATACATCGGCAAGGAAATAACCGGCAAGCAGATTGTAGCCCATAAAGAAGAAAATCCCGATGATAATCGACATATGACTTTCCGCGTGGGGATTGCCAAAATGCAACCCCAGCGCAATAAACAACACAATAACGTTTAAAATCAGCAAACCGGTTTTCGGTTCAGACACATTTAACGTTAAAAAAAGCCAAATTAATGCAGTCACAATACCGATAAAAATGGCAAAAATTAATaaa                                                                                                                                                 >  1:156349/1‑251 (MQ=255)                                                                                ccGTAAGAATCAAATACATCGGCAAGGAAATAACCGGCAAGCAGATTGTAGCCCATAAAGAAGAAAATCCCGATGATAATCGACATATGACTTTCCGCGTGGGGATTGCCAAAATGCAACCCCAGCGCAATAAACAACACAATAACGTTTAAAATCAGCAAACCGGTTTTCGGTTCAGACACATTTAACg                                                                                                                                                                                                            >  1:786651/1‑190 (MQ=255)                                                                                ccGTAAGAATCAAATACATCGGCAAGGAAATAACCGGCAAGCAGATTGTAGCCCATAAAGAAGAAAATCCCGATGATAATCGACATATGACTTTCCGCGTGGGGATTGCCAAAATGCAACCCCAGCGCAATAAACAACACAATAACGTTTAAAATCAGCAAACCGGTTTTCGGTTCAGACACATTTAACGTTAAAAAAAGCCAAAtt                                                                                                                                                                                           <  1:256712/207‑1 (MQ=255)                                                                                      gAATCAAATACATCGGCAAGGAAATAACCGGCAAGCAGATTGTAGCCCATAAAGAAGAAAATCCCGATGATAATCGACATATGACTTTCCGCGTGGGGATTGCCAAAATGCAACCCCAGCGCAATAAACAACACAATAACGTTTAAAATCAGCAAACCGGTTTTCGGTTCAGACACATTTAACGTTAAAAAAAGCCAAATTAATGc                                                                                                                                                                                      >  1:50449/1‑206 (MQ=255)                                                                                       aaTCAAATACATCGGCAAGGAAATAACCGGCAAGCAGATTGTAGCCCATAAAGAAGAAAATCCCGATGATAATCGACATATGACTTTCCGCGTGGGGATTGCCAAAATGCAACCCCAGCGCAATAAACAACACAATAACGTTTAAAATCAGCAAACCGGTTTTCGGTTCAGACACATTTAACGTTAAAAAAAGCCAAATTAATGCAGTCACAATACCGATAAAAATGGCAAAAATTAATAAAATGGACAg                                                                                                                                         <  1:161987/250‑1 (MQ=255)                                                                                        aTCAAATACATCGGCAAGGAAATAACCGGCAAGCAGATTGTAGCCCATAAAGAAGAAAATCCCGATGATAATCGACATATGACTTTCCGCGTGGGGATTGCCAAAATGCAACCCCAGCGCAATAAACAACACAATAACGTTTAAAATCAGCAAACCGGTTTTCGGTTCAGACACATTTAACGTTAAAAAAAGCCAAATTAATGCAGTCACAATACCGATa                                                                                                                                                                      <  1:244496/220‑1 (MQ=255)                                                                                              tACATCGGCAAGGAAATAACCGGCAAGCAGATTGTAGCCCATAAAGAAGAAAATCCCGATGATAATCGACATATGACTTTCCGCGTGGGGATTGCCAAAATGCAACCCCAGCGCAATAAACAACACAATAACGTTTAAAATCAGCAAACCGGTTTTCGGTTCAGACACATTTAACGTTAAAAAAAGCCAAATTAATGCAGTCACAATACCGATAAAAATGGCAAAAATTAATAAAATGGACAGTGCCCAAg                                                                                                                                 <  1:671682/251‑1 (MQ=255)                                                                                                cATCGGCAAGGAAATAACCGGCAAGCAGATTGTAGCCCATAAAGAAGAAAATCCCGATGATAATCGACATATGACTTTCCGCGTGGGGATTGCCAAAATGCAACCCCAGCGCAATAAACAACACAATAACGTTTAAAATCAGCAAACCGGTTTTCGGTTCAGACACATTTAACGTTAAAAAAAGCCAAATTAATGCAGTCACAATACCGATAAAAATGGCAAAAATTAATAAAATGGACAGTGCCCAAGaa                                                                                                                               <  1:576639/251‑1 (MQ=255)                                                                                                 aTCGGCAAGGAAATAACCGGCAAGCAGATTGTAGCCCATAAAGAAGAAAATCCCGATGATAATCGACATATGACTTTCCGCGTGGGGATTGCCAAAATGCAACCCCAGCGCAATAAACAACACAATAACGTTTAAAATCAGCAAACCGGTTTTCGGTTCAGACACATTTAACGTTAAAAAAAGCCAAATTAATGCAGTCACAATACCGATAAAAATGGCAAAAATTAATAAAATGGACAGTGCCCAAGaaa                                                                                                                              <  1:241512/251‑1 (MQ=255)                                                                                                    ggCAAGGAAATAACCGGCAAGCAGATTGTAGCCCATAAAGAAGAAAATCCCGATGATAATCGACATATGACTTTCCGCGTGGGGATTGCCAAAATGCAACCCCAGCGCAATAAACAACACAATAACGTTTAAAATCAGCAAACCGGTTTTCGGTTCAGACACATTTAACGTTAAAAAAAGCCAAATTAATGCAGTCACAATACCGATAAAAATGGCAAAAATTAATAAAATGGACAGTGCCCAAGAAATGt                                                                                                                           >  1:420255/1‑251 (MQ=255)                                                                                                                         cAGATTGTAGCCCATAAAGAAGAAAATCCCGATGATAATCGACATATGACTTTCCGCGTGGGGATTGCCAAAATGCAACCCCAGCGCAATAAACAACACAATAACGTTTAAAATCAGCAAACCGGTTTTCGGTTCAGACACATTTAACGTTAAAAAAAGCCAAATTAATGCAGTCACAATACCGATAAAAATGGCAAAAATTAATAAAATGGACAGTGCCCAAGAAATGTTCTTAAGGTGCGGATAAtgc                                                                                                       <  1:233575/250‑1 (MQ=255)                                                                                                                           gATTGTAGCCCATAAAGAAGAAAATCCCGATGATAATCGACATATGACTTTCCGCGTGGGGATTGCCAAAATGCAACCCCAGCGCAATAAACAACACAATAACGTTTAAAATCAGCAAACCGGTTTTCGGTTCAGACACATTTAACGTTAAAAAAAGCCAAATTAATGc                                                                                                                                                                                      <  1:351885/169‑1 (MQ=255)                                                                                                                            aTTGTAGCCCATAAAGAAGAAAATCCCGATGATAATCGACATATGACTTTCCGCGTGGGGATTGCCAAAATGCAACCCCAGCGCAATAAACAACACAATAACGTTTAAAATCAGCAAACCGGTTTTCGGTTCAGACACATTTAACGTTAAAAAAAGCCAAATTAATGCAGTCACAATACCGATAAAAATGGCAAAAATTAATAAAATGGACAGTGCCCAAGAAATGTTCTTAAGGTGCGGATAAtgctg                                                                                                     >  1:784674/1‑249 (MQ=255)                                                                                                                               gTAGCCCATAAAGAAGAAAATCCCGATGATAATCGACATATGACTTTCCGCGTGGGGATTGCCAAAATGCAACCCCAGCGCAATAAACAACACAATAACGTTTAAAATCAGCAAACCGGTTTTCGGTTCAGACACATTTAACGTTAAAAAAAGCCAAATTAATGCAGTCACAATACCGATAAAAATGGCAAAAATTAATAAAATGGACAGTGCCCAAGAAATGTTCTTAAg                                                                                                                    >  1:250960/1‑231 (MQ=255)                                                                                                                                   cccATAAAGAAGAAAATCCCGATGATAATCGACATATGACTTTCCGCGTGGGGATTGCCAAAATGCAACCCCAGCGCAATAAACAACACAATAACGTTTAAAATCAGCAAACCGGTTTTCGGTTCAGACACATTTAACGTTAAAAAAAGCCAAATTAATGCAGTCACAATACCGATAAAAATGGCAAAAATTAATAAAATGGACAGTGCCCAAGAAATGTTCTTAAGGTGCGGATAATGCTGCTTATacc                                                                                             <  1:405211/250‑1 (MQ=255)                                                                                                                                   cccATAAAGAAGAAAATCCCGATGATAATCGACATATGACTTTCCGCGTGGGGATTGCCAAAATGCAACCCCAGCGCAATAAACAACACAATAACGTTTAAAATCAGCAAACCGGTTTTCGGTTCAGACACATTTAACGTTAAAAAAAGCCAAATTAATGCAGTCACAATACCGATAAAAATGGCAAAAATTAATAAAATGGACAGTGCCCAAGAAATGTTCTTAAGGTGCGGATAATGCTGCTTATacc                                                                                             <  1:134782/250‑1 (MQ=255)                                                                                                                                   cccATAAAGAAGAAAATCCCGATGATAATCGACATATGACTTTCCGCGTGGGGATTGCCAAAATGCAACCCCAGCGCAATAAACAACACAATAACGTTTAAAATCAGCAAACCGGTTTTCGGTTCAGACACATTTAACGTTAAAAAAAGCCAAATTAATGCAGTCACAATACCGATAAAAATGGCAAAAATTAATAAAATGGACAGTGCCCAAGAAATGTTCTTAAGGTGCGGATAATGCTGCTTATac                                                                                              <  1:123968/249‑1 (MQ=255)                                                                                                                                   cccATAAAGAAGAAAATCCCGATGATAATCGACATATGACTTTCCGCGTGGGGATTGCCAAAATGCAACCCCAGCGCAATAAACAACACAATAACGTTTAAAATCAGCAAACCGGTTTTCGGTTCAGACACATTTAACGTTAAAAAAAGCCAAATTAATGCAGTCACAATACCGATAAAAATGGCAAAAATTAATAAAATGGACAGTGCCCAAGAAATGTTCTTAAGGTGCGGATAATGCTGCTTATac                                                                                              <  1:149266/249‑1 (MQ=255)                                                                                                                                            aagaaAATCCCGATGATAATCGACATATGACTTTCCGCGTGGGGATTGCCAAAATGCAACCCCAGCGCAATAAACAACACAATAACGTTTAAAATCAGCAAACCGGTTTTCGGTTCAGACACATTTAACGTTAAAAAAAGCCAAATTAATGCAGTCACAATACCGATAAAAATGGCAAAAATTAATAAAATGGACAGTGCCCAAGAAATGTTCTTAAGGTGCGGATAATGCTGCTTATACCAACCATGc                                                                                     <  1:280450/249‑1 (MQ=255)                                                                                                                                                  aTCCCGATGATAATCGACATATGACTTTCCGCGTGGGGATTGCCAAAATGCAACCCCAGCGCAATAAACAACACAATAACGTTTAAAATCAGCAAACCGGTTTTCGGTTCAGACACATTTAACGTTAAAAAAAGCCAAATTAATGCAGTCACAATACCGATAAAAATGGCAAAAATTAATAAAATGGACAGTGCCCAAGAAATGTTCTTAAGGTGCGGATAATGCTGCTTATaccaacc                                                                                         <  1:863595/239‑1 (MQ=255)                                                                                                                                                      cGATGATAATCGACATATGACTTTCCGCGTGGGGATTGCCAAAATGCAACCCCAGCGCAATAAACAACACAATAACGTTTAAAATCAGCAAACCGGTTTTCGGTTCAGACACATTTAACGTTAAAAAAAGCCAAATTAATGCAGTCACAATACCGATAAAAATGGCAAAAATTAATAAAATGGACAGTGCCCAAGAAATGTTCTTAAGGTGCGGATAATGCTGCTTATACCAACCATGCAACAACATAATg                                                                         <  1:558017/251‑1 (MQ=255)                                                                                                                                                           atAATCGACATATGACTTTCCGCGTGGGGATTGCCAAAATGCAACCCCAGCGCAATAAACAACACAATAACGTTTAAAATCAGCAAACCGGTTTTCGGTTCAGACACATTTAACGTTAAAAAAAGCCAAATTAATGCAGTCACAATACCGATAAAAATGGCAAAAATTAATAAAATGGACAGTGCCCAAGAAATGTTCTTAAGGTGCGGATAATGCTGCTTATACCAACCATGCAACAACATAATGGCaaa                                                                    >  1:633265/1‑251 (MQ=255)                                                                                                                                                                  aCATATGACTTTCCGCGTGGGGATTGCCAAAATGCAACCCCAGCGCAATAAACAACACAATAACGTTTAAAATCAGCAAACCGGTTTTCGGTTCAGACACATTTAACGTTAAAAAAAGCCAAATTAATGCAGTCACAATACCGATAAAAATGGCAAAAATTAATAAAATGGACAGTGCCCAAGAAATGTTCTTAAGGTGCGGATAATGCTGCTTATac                                                                                              >  1:891663/1‑218 (MQ=255)                                                                                                                                                                  aCATATGACTTTCCGCGTGGGGATTGCCAAAATGCAACCCCAGCGCAATAAACAACACAATAACGTTTAAAATCAGCAAACCGGTTTTCGGTTCAGACACATTTAACGTTAAAAAAAGCCAAATTAATGCAGTCACAATACCGATAAAAATGGCAAAAATTAATAAAATAGACAGTGCCCAAGAAATGTTCTTAAGGTGCGGATAATGCTGCTTATac                                                                                              >  1:433714/1‑218 (MQ=255)                                                                                                                                                                  aCATATGACTTTCCGCGTGGGGATTGCCAAAATGCAACCCCAGCGCAATAAACAACACAATAACGTTTAAAATCAGCAAACCGGTTTTCGGTTCAGACACATCTAACGTTAAAAAAAGCCAAATTAATGCAGTCACAATACCGATAAAAATGGCAAAAATTAATAAAATGGACAGTGCCCAAGAAATGTTCTTAAg                                                                                                                    >  1:663465/1‑196 (MQ=255)                                                                                                                                                                    atatGACTTTCCGCGTGGGGATTGCCAAAATGCAACCCCAGCGCAATAAACAACACAATAACGTTTAAAATCAGCAAACCGGTTTTCGGTTCAGACACATTTAACGTTAAAAAAAGCCAAATTAATGCAGTCACAATACCGATAAAAATGGCAAAAATTAATAAAATGGACAGTGCCCAAGAAATGTTCTTAAGGTGCGGATAATGCTGCTTATTCCAACCATGCAACAACATAATGGCAAAGATCGtaat                                                           <  1:498997/251‑1 (MQ=255)                                                                                                                                                                    atatGACTTTCCGCGTGGGGATTGCCAAAATGCAACCCCAGCGCAATAAACAACACAATAACGTTTAAAATCAGCAAACCGGTTTTCGGTTCAGACACATTTAACGTTAAAAAAAGCCAAATTAATGCAGTCACAATACCGATAAAAATGGCAAAAATTAATAAAATGGACAGTGCCCAAGAAATGTTCTTAAGGTGCGGATAATGCTGCTTATACCAACCATGCAACAACATAATGGCAAAGATCGtaat                                                           >  1:72141/1‑251 (MQ=255)                                                                                                                                                                      atGACTTTCCGCGTGGGGATTGCCAAAATGCAACCCCAGCGCAATAAACAACACAATAACGTTTAAAATCAGCAAACCGGTTTTCGGTTCAGACACATTTAACGTTAAAAAAAGCCAAATTAATGCAGTCACAATACCGATAAAAATGGCAAAAATTAATAAAATGGAc                                                                                                                                           >  1:568552/1‑169 (MQ=255)                                                                                                                                                                                           gCCAAAATGCAACCCCAGCGCAATAAACAACACAATAACGTTTAAAATCAGCAAACCGGTTTTCGGTTCAGACACATTTAACGTTAAAAAAAGCCAAATTAATGCAGTCACAATACCGATAAAAATGGCAAAAATTAATAAAATGGACAGTGCCCAAGAAATGTTCTTAAGGTGCGGATAATGCTGCTTATACCAACCATGCAACAACATAATGGCAAAGATCGTAATAATAAAAAGCAACAGGCCTGCCa                                    >  1:869777/1‑251 (MQ=255)                                                                                                                                                                                            ccAAAATGCAACCCCAGCGCAATAAACAACACAATAACGTTTAAAATCAGCAAACCGGTTTTCGGTTCAGACACATTTAACGTTAAAAAAAGCCAAATTAATGCAGTCACAATACCGATAAAAATGGCAAAAATTAATAAAATGGACAGTGCCCAAGAAATGTTCTTAAGGTGCGGATAATGCTGCTTATACCAACCATGCAACAACATAATGGCAAAGATCGTAATAATAAAAAGCAACAGGCCTGCCa                                    <  1:725803/250‑1 (MQ=255)                                                                                                                                                                                                 aTGCAACCCCAGCGCAATAAACAACACAATAACGTTTAAAATCAGCAAACCGGTTTTCGGTTCAGACACATTTAACGTTAAAAAAAGCCAAATTAATGCAGTCACAATACCGATAAAAATGGCAAAAATtaataa                                                                                                                                                  >  1:148686/1‑135 (MQ=255)                                                                                                                                                                                                      aCCCCAGCGCAATAAACAACACAATAACGTTTAAAATCAGCAAACCGGTTTTCGGTTCAGACACATTTAACGTTAAAAAAAGCCAAATTAATGCAg                                                                                                                                                                                    >  1:483752/1‑96 (MQ=255)                                                                                                                                                                                                       ccccAGCGCAATAAACAACACAATAACGTTTAAAATCAGCAAACCGGTTTTCGGTTCAGACACATTTAACGTTAAAAAAAGCCAAATTAATGCAGTCACAATACCGATAAAAATGGCAAAAATTAATAAAATGGACAGTGCCCAAGAAATGTTCTTAAGGTGCGGATAATGCTGCTTATACCAACCATGc                                                                                     >  1:581284/1‑190 (MQ=255)                                                                                                                                                                                                              gcAATAAACAACACAATAACGTTTAAAATCAGCAAACCGGTTTTCGGTTCAGACACATTTAACGTTAAAAAAAGCCAAATTAATGCAGTCACAATACCGATAAAAATGGCAAAAATTAATAAAATGGACAGTGCCCAAGAAATGTTCTTAAGGTGCGGATAATGCTGCTTATACCAACCATGCAACAACAt                                                                             >  1:286938/1‑191 (MQ=255)                                                                                                                                                                                                              gcAATAAACAACACAATAACGTTTAAAATCAGCAAACCGGTTTTCGGTTCAGACACATTTAACGTTAAAAAAAGCCAAATTAATGCAGTCACAATACCGATAAAAATGGCAAAAATTAATAAAATGGACAGTGCCCAAGAAATGTTCTTAAGGTGCGGATAATGCTGCTTATACCAACCATGCAACAACATAAt                                                                          >  1:209850/1‑194 (MQ=255)                                                                                                                                                                                                               cAATAAACAACACAATAACGTTTAAAATCAGCAAACCGGTTTTCGGTTCAGACACATTTAACGTTAAAAAAAGCCAAATTAATGCAGTCACAATACCGa                                                                                                                                                                        >  1:473049/1‑99 (MQ=255)                                                                                                                                                                                                                        acacAATAACGTTTAAAATCAGCAAACCGGTTTTCGGTTCAGACACATTTAACg                                                                                                                                                                                                            <  1:606026/54‑1 (MQ=255)                                                                                                                                                                                                                        acacAATAACGTTTAAAATCAGCAAACCGGTTTTCGGTTCAGACACATTTAACGTTAAAAAAAGCCAAATTAATGCAGTCACAATACCGATAAAAATGGCAAAAATTAATAAAATGGACAGTGCCCAAGAAATGTTCTTAAGGTGCGGATAATGCTGCTTATacc                                                                                             <  1:300491/165‑1 (MQ=255)                                                                                                                                                                                                                        acacAATAACGTTTAAAATCAGCAAACCGGTTTTCGGTTCAGACACATTTAACGTTAAAAAAAGCCAAATTAATGCAGTCACAATACCGATAAAAATGGCAAAAATTAATAAAATGGACAGTGCCCAAGAAATGTTCTTAAGGTGCGGATAATGCTGCTTATACCAACCATGCAACAACATAATGGCAAAGAt                                                                 <  1:366644/193‑1 (MQ=255)                                                                                                                                                                                                                        acacAATAACGTTTAAAATCAGCAAACCGGTTTTCGGTTCAGACACATTTAACGTTAAAAAAAGCCAAATTAATGCAGTCACAATACCGATAAAAATGGCAAAAATTAATAAAATGGACAGTGCCCAAGAAATGTTCTTAAGGTGCGGATAATGCTGCTTATACCAACCATGCAACAACATAATGGCAAAGAt                                                                 >  1:472758/1‑193 (MQ=255)                                                                                                                                                                                                                        acacAATAACGTTTAAAATCAGCAAACCGGTTTTCGGTTCAGACACATTTAACGTTAAAAAAAGCCAAATTAATGCAGTCACAATACCGATAAAAATGGCAAAAATTAATAAAATGGACAGTGCCCAAGAAATGTTCTTAAGGTGCGGATAATGCTGCTTATACCAACCATGCAACAACATAATGGCAAAGATCGtaat                                                           >  1:847954/1‑199 (MQ=255)                                                                                                                                                                                                                        acacAATAACGTTTAAAATCAGCAAACCGGTTTTCGGTTCAGACACATTTAACGTTAAAAAAAGCCAAATTAATGCAGTCACAATACCGATAAAAATGGCAAAAATTAATAAAATGGACAGTGCCCAAGAAATGTTCTTAAGGTGCGGATAATGCTGCTTATACCAACCATGCAACAACATAATGGCAAAGATCGTAATAATAAAAAGCAACAGGCCTGCCAATACGGCAAAATc                       <  1:769862/235‑1 (MQ=255)                                                                                                                                                                                                                             aTAACGTTTAAAATCAGCAAACCGGTTTTCGGTTCAGACACATTTAACGTTAAAAAAAGCCAAATTAATGCAGTCACAATACCGATAAAAATGGCAAAAATTAATAGAATGGACAGTGCCCAAGAAATGTTCTTAAGGTGCGGATAATGCTGCTTATACCAACCATGCAACAACATAATGGCAAAGATCGTAATAATAAAAAGCAACAGGCCTGCCAATACGGCAAAATCACCGGAATTTACATTggaaaa  >  1:331402/1‑251 (MQ=255)                                                                                                                                                                                                                                 cGTTTAAAATCAGCAAACCGGTTTTCGGTTCAGACACATTTAACGTTAAAAAAAGCCAAATTAATGCAGTCACAATACCGATAAAAATGGCAAAAATTAATAAAATGGACAGTGCCCAAGAAATGTTCTTAAGGTGCGGATAAtgc                                                                                                       <  1:843779/146‑1 (MQ=255)                                                                                                                                                                                                                                 cGTTTAAAATCAGCAAACCGGTTTTCGGTTCAGACACATTTAACGTTAAAAAAAGCCAAATTAATGCAGTCACAATACCGATAAAAATGGCAAAAATTAATAAAATGGACAGTGCCCAAGAAATGTTCTTAAGGTGCGGATAATGCTGCTTATACCAACCATGCAACAACAt                                                                             <  1:833306/172‑1 (MQ=255)                                                                                                                                                                                                                                           cAGCAAACCGGTTTTCGGTTCAGACACATTTAACGTTAAAAAAAGCCAAATTAATGCAGTCACAATACCGATAAAAATGGCAAAAATTAATAAAATGGACAGTGCCCAAGaa                                                                                                                               <  1:865101/112‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                    GTTCTCTTTGCGTAAAAAATAAAGCATAGAAATTATCGCAGTAAACAATAACGGTACATCACAGGTTAAGGCGCCCTCACCGTAAGAATCAAATACATCGGCAAGGAAATAACCGGCAAGCAGATTGTAGCCCATAAAGAAGAAAATCCCGATGATAATCGACATATGACTTTCCGCGTGGGGATTGCCAAAATGCAACCCCAGCGCAATAAACAACACAATAACGTTTAAAATCAGCAAACCAGTTTTCGGTTCAGACACATTTAACGTTAAAAAAAGCCAAATTAATGCAGTCACAATACCGATAAAAATGGCAAAAATTAATAAAATGGACAGTGCCCAAGAAATGTTCTTAAGGTGCGGATAATGCTGCTTATACCAACCATGCAACAACATAATGGCAAAGATCGTAATAATAAAAAGCAACAGGCCTGCCAATACGGCAAAATCACCGGAATTTACATTGGAAAA  >  1/1855015‑1855485

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑