Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,832,688	G→A	intergenic (+50788/+2696)	unknown → / ← unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,832,688	0	G	A	100.0%	201.9 / NA	72	intergenic (+50788/+2696)	unknown/unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (32/40);  total (32/40)

ATATCCGCCACCATCACAATAATGTCTTTCGGCGTTAAATCAGGATTC‑TGTTGCAGCAAATGCAGCAAATAATCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAAGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGTGCCGTTCGGTGTTAAATGCAAAATACGATGTTGG‑ATCTGGCTTAATAAATTGTGCGGCATGATTTCCACATAGGCGGAAATGTCATTGGGTTCCAGCTGCGCCAATAAGTAGGAAAAATCCCGTCCCAGCTTACCCCAAGATGCCAATAAGGAATTACCCACCTGTAATTTTTCCTGCGCATAAGTCAGCTCCCAATGTTGCTGCGCCTGTGCAGCCTGTTCCGCCGATAATAAAGGCTTATCTGCGTGATTAAAATAATCGGTGCGAGTACGCAACGCCAGTTTTTCTACGAATTTCGGATCAACGATGTCGCCCCAATATTC  >  1/1832446‑1832930                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                   atatCCGCCACCATCACAATAATGTCTTTCGGCGTTAAATC‑GGGTTCTTGTTGCAACAAATGTAGCAAATAATCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTggg                                                                                                                                                                                                                                            <  1:84990/251‑1 (MQ=255)  tatCCGCCACCATCACAATAATGTCTTTCGGCGTTAAATC‑GGGTTCTTGTTGCAACAAATGTAGCAAATAATCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGGCATTCGGAGTTAAATGCAAAATACGGGGTTGG‑ATTTTGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTgggg                                                                                                                                                                                                                                           >  1:516569/1‑250 (MQ=255)        ccaccaTCACAATAATGTCTTTCGGCGTTAAATC‑GGGTTCTTGTTGCAACAAATGTAGCAAATAATCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGc                                                                                                                                                                                                                                     <  1:190776/251‑1 (MQ=255)        ccaccaTCACAATAATGTCTTTCGGCGTTAAATC‑GGGTTCTTGTTGCAACAAATGTAGCAAATAATCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGc                                                                                                                                                                                                                                     <  1:191723/251‑1 (MQ=255)         caccaTCACAATAATGTCTTTCGGCGTTAAATC‑GGGTTCTTGTTGCAACAAATGTAGCAAATAATCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCt                                                                                                                                                                                                                                    <  1:168638/251‑1 (MQ=255)              tCACAATAATGTCTTTCGGCGTTAAATC‑GGGTTCTTGTTGCAACAAATGTAGCAAATAATCCCGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTgcgc                                                                                                                                                                                                                                >  1:386103/1‑250 (MQ=255)                 caATAATGTCTTTCGGCGTTAAATC‑GGGTTCTTGTTGCAACAAATGTAGCAAATAATCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGGGTTAAATGCAAAATACGGCGTTTG‑GTTTGGCCTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTGCGCCAAt                                                                                                                                                                                                                            >  1:149453/1‑251 (MQ=255)                 caATAATGTCTTTCGGCGTTAAATC‑GGGTTCTTGTTGCAACAAATGTAGCAAATAATCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTGCGCCAAt                                                                                                                                                                                                                            >  1:583023/1‑251 (MQ=255)                   ataatGTCTTTCGGCGTTAAATC‑GGGTTCTTGTTGCAACAAATGTAGCAAATAATCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTACCGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGG‑ATTTGGCCTAATAAATTGTGCGGCGGGATTTCCACATAGGCAGAAATATCATTGGGTTCCCGCTGCGCCCATaa                                                                                                                                                                                                                          >  1:690580/1‑251 (MQ=255)                     aatGTCTTTCGGCGTTAAATC‑GGGTTCTTGTTGCAACAAATGTAGCAAATAATCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTGCGCCAATAAGt                                                                                                                                                                                                                        <  1:441935/251‑1 (MQ=255)                                gCGTTAAATC‑GGGTTCTTGTTGCAACAAATGTAGCAAATAATCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTGCGCCAATAAGTAGGAAAAATc                                                                                                                                                                                                              <  1:773171/250‑1 (MQ=255)                                     taaatcgggttcttGTTGCAACAAATGTAGCAAATAATCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTGCGCCAATAAGTAGGAAAAATCCCGTc                                                                                                                                                                                                         <  1:691200/238‑1 (MQ=255)                                       aatcgggttcttGTTGCAACAAATGTAGCAAATAATCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCa                                                                                                                                                                                                                                                 <  1:344305/198‑1 (MQ=255)                                             gttcttGTTGCAACAAATGTAGCAAATAATCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTGCGCCAATAAGTAGGAAAAATCCCGTCCCAGCtt                                                                                                                                                                                                  <  1:442252/245‑1 (MQ=255)                                                               gtagcaaatAATCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTGCGCCAATAAGTAGGaa                                                                                                                                                                                                                   <  1:402332/213‑1 (MQ=255)                                                                    aaatAATCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTGCGCCAATAAGTAGGaaaa                                                                                                                                                                                                                 <  1:776337/212‑1 (MQ=255)                                                                    aaatAATCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTGCGCCAATAAGTAGGAAAAATcc                                                                                                                                                                                                             <  1:80016/216‑1 (MQ=255)                                                                       taatCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTGCGCCAATAAGTAGGAAAAATCCCGTCCCAGCTTAcccc                                                                                                                                                                                             <  1:379229/229‑1 (MQ=255)                                                                        aatCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTGCGCCAATAAGTAGGAAAAATCCCGTCCCAGCTTACCCCAAGATGCCAATAAGGAATTAccc                                                                                                                                                                      <  1:900332/251‑1 (MQ=255)                                                                        aatCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTGCGCCAATAAGTAGGAAAAATCCCGTCCCAGCTTACCCCAAGATGCCAATAAGGAATTAccc                                                                                                                                                                      <  1:1874/251‑1 (MQ=255)                                                                          tCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTGCGCCAATAAGTAGGAAAAATCCCGTCCCAGCTTACCCCAAGATGCCAATAAGGAATTAcc                                                                                                                                                                       <  1:375139/248‑1 (MQ=255)                                                                                 acacTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTGCGCCAATAAGTAGGAAAAATCCCGTCCCAGCTTACCCCAAGATGCCAATAAGGAATTACCCACCTGTAAt                                                                                                                                                             <  1:42368/251‑1 (MQ=255)                                                                                  cacttccacttcGCGCATCATGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTGCGCCAATAAGTAGGAAAAATCCCGTCCCAGCTTACCCCAAGATGCCAATAAGGAATTAccc                                                                                                                                                                      <  1:80598/241‑1 (MQ=255)                                                                                                 catcatGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTGCGCCAATAAGTAGGAAAAATCCCGTCCCAGCTTACCCCAAGATGCCAATAAGGAATTACCCACCTGTAATTTTTCCTGCGCATAAg                                                                                                                                             >  1:470966/1‑251 (MQ=255)                                                                                                   tcatGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTGCGCCAATAAGTAGGAAAAATCCCGTCCCAGCTTACCCCAAGATGCCAATAAGGAATTACCCACCTGTAATTTTTCCTGCGCATAAGTc                                                                                                                                           <  1:729703/251‑1 (MQ=255)                                                                                                   tcatGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTGCGCCAATAAGTAGGAAAAATCCCGTCCCAGCTTACCCCAAGATGCCAATAAGGAATTACCCACCTGTAATTTTTCCTGCGCATAAGTc                                                                                                                                           <  1:626851/251‑1 (MQ=255)                                                                                                     atGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGG‑ATTTGGCTTAATAAATTTTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTGCGCCAATAAGTAGGAAAAATCCCGTCCCAGCTTACCCCAAGATGCCAATAAGGAATTACCCACCTGTAATTTTTCCCGCGCATAAGTCAg                                                                                                                                         >  1:502266/1‑251 (MQ=255)                                                                                                     atGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTGCGCCAATAAGTAGGAAAAATCCCGTCCCAGCTTACCCCAAGATGCCAATAAGGAATTACCCACCTGTAATTTTTCCTGCGCATAAGTCAg                                                                                                                                         >  1:551369/1‑251 (MQ=255)                                                                                                        cTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTGCGCCAATAAGTAGGAAAAATCCCGTCCCAGCTTACCCCAAGa                                                                                                                                                                                         >  1:501251/1‑200 (MQ=255)                                                                                                                 gAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTGCGCCAATAAGTAGGAAAAATCCCGTCCCAGCTTACCCCAAGATGCCAATAAGGAATTACCCACCTGTAATTTTTCCTGCGCATAAGTCAGCTCCCAATGTtg                                                                                                                             >  1:256013/1‑251 (MQ=255)                                                                                                                  aaTGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTGCGCCAATAAGTAGGAAAAATCCCGTCCCAGCTTACCCCAAGATGCCAATAAGGAATTACCCACCTGTAATTTTTCCTGCGCATAAGTCAGCTCCCAATGTtgc                                                                                                                            <  1:263270/251‑1 (MQ=255)                                                                                                                  aaTGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTGCGCCAATAAGTAGGAAAAATCCCGTCCCAGCTTACCCCAAGATGCCAATAAGGAATTACCCACCTGTAATTTTTCCTGCGCATAAGTCAGCTCCCAATGTtgc                                                                                                                            <  1:161007/251‑1 (MQ=255)                                                                                                                         ggTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTGCGCCAATAAGTAGGAAAAATCCCGTCCCAGCTTACCCCAAGATGCCAATAAGGAATTACCCACCTGTAATTTTTCCTGCGCATAAGTc                                                                                                                                           <  1:530973/228‑1 (MQ=255)                                                                                                                          gTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTTCGCCAATAAGTAGGAAAAATCCCGTCCCAGCTTACCCCAAGATGCCAATAAGGAATTACCCACCTGTAATTTTTCCTGCGCATAAGGCAGCTCCCAATGTTGCTgcgc                                                                                                                       >  1:241125/1‑248 (MQ=255)                                                                                                                             aaaGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTGCGCCAATAAGTAGGAAAAATCCCGTCCCAGCTTACCCCAAGATGCCAATAAGGAATTACCCACCTGTAATTTTTCCTGCGCATAAGTCAGCTCCCAATGTTGCTGCGCCTGTGc                                                                                                                 <  1:233331/251‑1 (MQ=255)                                                                                                                                       cATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTGCGCCAATAAGTAGGAAAAATCCCGTCCCAGCTTACCCCAAGATGCCAATAAGGAATTACCCACCTGTAATTTTTCCTGCGCATAAGTCAGCTCCCAATGTTGCTGCGCCTGTGCAGCCTGTTc                                                                                                        >  1:98117/1‑250 (MQ=255)                                                                                                                                            tCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGG‑ATTTGGCTTAATAAATTGGGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTGCGCCAATAAGTAGGAAAAATCCCGTCCCAGCTTACCCCAAGATGCCAATAAGGAATTACCCACCTGTAATTTTTCCTGCGCATAAGTCAGCTCCCAATGTTGCTGCGCCTGTGCAGCCTGTTCCGCCGa                                                                                                  >  1:316100/1‑251 (MQ=255)                                                                                                                                                  tAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTGCGCCAATAAGTAGGAAAAATCCCGTCCCAGCTTACCCCAAGATGCCAATAAGGAATTACCCACCTGTAATTTTTCCTGCGCATAAGTCAGCTCCCAATGtt                                                                                                                              <  1:514147/217‑1 (MQ=255)                                                                                                                                                  tAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTGCGCCAATAAGTAGGAAAAATCCCGTCCCAGCTTACCCCAAGATGCCAATAAGGAATTACCCACCTGTAATTTTTCCTGCGCATAAGTCAGCTCCCAATGTTGCTGCGCCTGTGCAGCCTGTTCCGCCGAtaataa                                                                                            >  1:510438/1‑251 (MQ=255)                                                                                                                                                  tAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGGTGGAATTTGG‑TTAATAAATTGAGCGGCGTGATTTCCACAAAGGCAGAAATATCATTCGGTTTCAGCTGCGCCTTTAAGTAGGAAAAATCCCGTCCCAGCCTACCCCAAGATGCCAATAAGGAATTACCCACCTGTAATTTTTCCTGCGCATAAGTCAGCTCCCAATg                                                                                                                                >  1:640273/1‑215 (MQ=255)                                                                                                                                                    gTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTGCGCCAATAAGTAGGAAAAATCCCGTCCCAGCTTACCCCAAGATGCCAATAAGGAATTACCCACCTGTAATTTTTCCTGCGCATAAGTCAGCTCCCAATGTTGCTGCGCCTGTGCAGCCTGTTCCGCCGATAATAAAg                                                                                          >  1:68301/1‑251 (MQ=255)                                                                                                                                                    gTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTGCGCCAATAAGTAGGAAAAATCCCGTCCCAGCTTACCCCAAGATGCCAATAAGGAATTACCCACCTGTAATTTTTCCTGCGCATAAGTCAGCTCCCAATGTTGCTGCGCCTGTGCAGCCTGTTCCGCCGATAATAAAg                                                                                          >  1:734751/1‑251 (MQ=255)                                                                                                                                                    gTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTGCGCCAATAAGTAGGAAAAATCCCGTCCCAGCTTACCCCAAGATGCCAATAAGGAATTACCCACCTGTAATTTTTCCTGCGCATAAGTCAGCTCCCAATGTTGCTGCGCCTGTGCAGCCTGTTCCGCCGATAATAAAg                                                                                          >  1:329665/1‑251 (MQ=255)                                                                                                                                                            gCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTGCGCCAATAAGTAGGAAAAATCCCGTCCCAGCTTACCCCAAGATGCCAATAAGGAATTACCCACCTGTAATTTTTCCTGCGCATAAGTCAGCTCCCAATGTTGCTGCGCCTGTGCAGCCTGTTCCGCCGATAATAAAg                                                                                          <  1:42506/243‑1 (MQ=255)                                                                                                                                                            gCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTGCGCCAATAAGTAGGAAAAATCCCGTCCCAGCTTACCCCAAGATGCCAATAAGGAATTACCCACCTGTAATTTTTCCTGCGCATAAGTCAGCTCCCAATGTTGCTGCGCCTGTGCAGCCTGTTCCGCCGATAATAAAGGCTTATc                                                                                   <  1:548998/250‑1 (MQ=255)                                                                                                                                                            gCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTGCGCCAATAAGTAGGAAAAATCCCGTCCCAGCTTACCCCAAGATGCCAATAAGGAATTACCCACCTGTAATTTTTCCTGCGCATAAGTCAGCTCCCAATGTTGCTGCGCCTGTGCAGCCTGTTCCGCCGATAATAAAGGCTTATc                                                                                   >  1:346913/1‑250 (MQ=255)                                                                                                                                                            gCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTGCGCCAATAAGTAGGAAAAATCCCGTCCCAGCTTACCCCAAGATGCCAATAAGGAATTACCCACCTGTAATTTTTCCTGCGCATAAGTCAGCTCCCAATGTTGCTGCGCCTGTGCAGCCTGTTCCGCCGATAATAAAGGCTTATc                                                                                   <  1:66807/250‑1 (MQ=255)                                                                                                                                                             cGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTGCGCCAATAAGTAGGAAAAATCCCGTCCCAGCTTACCCCAAGATGCCAATAAGGAATTACCCACCTGTAATTTTTCCTGCGCATAAGTCAGCTCCCAATGTTGCTGCGCCTGTGCAGCCTGTTCCGCCGATAATAAAGGCTTATCTg                                                                                 <  1:126236/251‑1 (MQ=255)                                                                                                                                                                     cattCGGAGTTAAATGCAAAATACGGTGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTGCGCCAATAAGTAGGAAAAATCCCGTCCCAGCTTACCCCAAGATGCCAATAAGGAATTACCCACCTGTAATTTTTCCTGCGCATAAGTCAGCTCCCAATGTTGCTGCGCCTGTGCAGCCTGTTCCGCCGATAATAAAGGCTTATCTGCGTGATTa                                                                         <  1:404184/249‑1 (MQ=255)                                                                                                                                                                      attCGGAGTTAAATGCAAAATACGGTGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTGCGCCAATAAGTAGGAAAAATCCCGTCCCAGCTTACCCCAAGATGCCAATAAGGAATTACCCACCTGTAATTTTTCCTGCGCATAAGTCAGCTCCCAATGTtgctg                                                                                                                          >  1:489024/2‑201 (MQ=255)                                                                                                                                                                      attCGGAGTTAAATGCAAAATACGGTGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTGCGCCAATAAGTAGGAAAAATCCCGTCCCAGCTTACCCCAAGATGCCAATAAGGAATTACCCACCTGTAATTTTTCCTGCGCATAAGTCAGCTCCCAATGTTGCTgcgc                                                                                                                       >  1:290745/2‑204 (MQ=255)                                                                                                                                                                             gtTAAATGCAAAATACGGTGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTGCGCCAATAAGTAGGAAAAATCCCGTCCCAGCTTACCCCAAGATGCCAATAAGGAATTACCCACCTGTAATTTTTCCTGCGCATAAGTCAGCTCCCAATGTTGCTGCGCCTGTGCAGCCTGTTCCGCCGATAATAAAGGCTTATCTGCGTGATTAAaataat                                                                  >  1:876436/1‑250 (MQ=255)                                                                                                                                                                                    gCAAAATACGGTGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTGCGCCAATAAGTAGGAAAAATCCCGTCCCAGCTTACCCCAAGATGCCAATAAGGAATTACCCACCTGTAATTTTTCCTGCGCATAAGTCAGCTCCCAATGTTGCTGCGCCTGTGCAGCCTGTTCCGCCGATAATAAAGGCTTATCTGCGTGATTAAAATAATCGGTGCGa                                                          >  1:376/1‑251 (MQ=255)                                                                                                                                                                                      aaaaTACGGTGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTGCGCCAATAAGTAGGAAAAATCCCGTCCCAGCTTACCCCAAGATGCCAATAAGGAATTACCCACCTGTAATTTTTCCTGCGCATAAGTCAGCTCCCAATGTTGCTGCGCCTGTGCAGCCTGTTCCGCCGATAATAAAGGCTTATCTGCGTGATTAAAATAATCGGTGCGAGt                                                        >  1:729477/1‑251 (MQ=255)                                                                                                                                                                                       aaaTACGGTGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTGCGCCAATAAGTAGGAAAAATCCCGTCCCAGCTTACCCCAAGATGCCAATAAGGAATTACCCACCTGTAATTTTTCCTGCGCATAAGTCAGCTCCCAATGTTGCTGCGCCTGTGCAGCCTGTTCCGCCGATAATAAAGGCTTATCTGCGTGATTAAAATAATCGGTGCGAGTa                                                       <  1:283552/251‑1 (MQ=255)                                                                                                                                                                                        aaTACGGTGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTGCGCCAATAAGTAGGAAAAATCCCGTCCCAGCTTACCCCAAGATGCCAATAAGGAATTACCCACCTGTAATTTTTCCTGCGCATAAGTCAGCTCCCAATGTTGCTGCGCCTGTGCAGCCTGTTCCGCCGATAATAAAGGCTTATCTGCGTGATTAAAATAATCGGTGCGAGTAc                                                      <  1:841177/251‑1 (MQ=255)                                                                                                                                                                                         gtACGGTGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTGCGCCAATAAGTAGGAAAAATCCCGTCCCAGCTTACCCCAAGATGCCAATAAGGAATTACCCACCTGTAATTTTTCCTGCGCATAAGTCAGCTCCCAATGTTGCTGCGCCTGTGCAGCCTGTTCCGCCGATAATAAAGGCTTATCTGCGTGATTAAaat                                                                     >  1:510962/2‑235 (MQ=255)                                                                                                                                                                                              gtGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTGCGCCAATAAGTAGGAAAAATCCCGTCCCAGCTTACCCCAAGATGCCAATAAGGAATTACCCACCTGTAATTTTTCCTGCGCATAAGTc                                                                                                                                           <  1:884644/159‑1 (MQ=255)                                                                                                                                                                                              gtGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTGCGCCAATAAGTAGGAAAAATCCCGTCCCAGCTTACCCCAAGATGCCAATAAGGAATTACCCACCTGTAATTTTTCCTGCGCATAAGTCAGCTCCCAATGTTGCTGCGCCTGTGc                                                                                                                 >  1:894001/2‑186 (MQ=255)                                                                                                                                                                                                   gg‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTGCGCCAATAAGTAGGAAAAATCCCGTCCCAGCTTACCCCAAGATGCCAATAAGGAATTACCCACCTGTAATTTTTCCTGCGCATAAGTCAGCTCCCAATGTTGCTGCGCCTGTGCAGCCTGTTCCGCCGATAATAAAGGCTTATCTGCGTGATTAAAATAATCGGTGCGAGTACGCAACGCCAGt                                           >  1:220176/1‑251 (MQ=255)                                                                                                                                                                                                      atttGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATAGCATTGGGTTCCAGCTGCGCCAATAAGTAGGAAAAATCCCGTCCCAGCTTACCCCAAGATGCCAATAAGGAATTACCCACCTGTAATTTTTCCTGCGCATAAGTCAGCTCCCAATGTTGCTGCGCCTGTGCAGCCTGTTCCGCCGATAATAAAGGCTTATCTGCGTGATTAAAATAATCGGTGCGAGTACGCAACGCCAGtt                                          <  1:763437/247‑1 (MQ=255)                                                                                                                                                                                                          ggCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTGCGCCAATAAGTAGGAAAAATCCCGTCCCAGCTTACCCCAAGATGCCAATAAGGAATTACCCACCTGTAATTTTTCCTGCGCATAAGTCAGCTCCCAATGTTGCTGCGCCTGTGCAGCCTGTTCCGCCGATAATAAAGGCTTATCTGCGTGATTAAAATAATCGGTGCGAGTACGCAACGCCAGttttt                                       >  1:631672/1‑249 (MQ=255)                                                                                                                                                                                                               aataaATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTGCGCCAATAAGTAGGAAAAATCCCGTCCCAGCTTACCCCAAGATGCCAATAAGGAATTACCCACCTGTAATTTTTCCTGCGCATAAGTCAGCTCCCAATGTTGCTGCGCCTGTGCAGCCTGTTCCGCCGATAATAAAGGCTTATCTGCGTGATTAAAATAATCGGTGCGAGTACGCAACGCCAGTTTTTCTACGaa                                >  1:98604/1‑251 (MQ=255)                                                                                                                                                                                                                        tgCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTGCGCCAATAAGTAGGAAAAATCCCGTCCCAGCTTACCCCAAGATGCCAATAAGGAATTACCCACCTGTAATTTTTCCTGCGCATAAGTCAGCTCCCAATGTTGCTGCGCCTGTGCAGCCTGTTCCGCCGATAATAAAGGCTTATCTGCGTGATTAAAATAATCGGTGCGAGTACGCAACGCCAGTTTTTCTACGAATTTCGGATc                       <  1:250919/251‑1 (MQ=255)                                                                                                                                                                                                                                gATTTCCACATAGGCAGAAATATCATTGGGCTCCAGCTGCGCCAATAAGTAGGAAAAATCCCGTCCCAGCTTACCCCAAGATGCCAATAAGGAATTACCCACCTGTAAtt                                                                                                                                                            <  1:354211/110‑1 (MQ=255)                                                                                                                                                                                                                                 aTTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTGCGCCAATAAGTAGGAAAAATCCCGTCCCAGCTTACCCCAAGATGCCAATAAGGAATTACCCACCTGTAATTTTTCCTGCGCATAAGTCAGCTCCCAATGTTGCTGCGCCTGTGCAGCCTGTTCCGCCGATAATAAAGGCTTATCTGCGTGATTAAAATAATCGGTGCGAGTACGCAACGCCAGTTTTTCTACGAATTTCGGATCAACGATGt               >  1:612310/1‑250 (MQ=255)                                                                                                                                                                                                                                      cacaTAGGCAGAAATATCATTGGGTTCCAGCTGCGCCAATAAGTAGGAAAAATCCCGTCCCAGCTTACCCCAAGATGCCAATAAGGAATTACCCACCTGTAATTTTTCCTGCGCATAAGTCAGCTCCCAATGTTGCTGCGCCTGTGCAGCCTGTTCCGCCGATAATAAAGGCTTATCTGCGTGATTAAAATAATc                                                                 <  1:855741/195‑1 (MQ=255)                                                                                                                                                                                                                                           aGGCAGAAATATCATTGGGTTCCAGCTGCGCCAATAAGTAGGAAAAATCCCGTCCCAGCTTACCCCAAGATGCCAATAAGGAATTACCCACCTGTAATTTTTCCTGCGCATAAGTCAGCTCCCAATGTTGCTGCGCCTGTGCAGCCTGTTCCGCCGATAATAAAGGCTTATCTGCGTGATTAAAATAATCGGTGCGAGTACGc                                                    >  1:3224/1‑203 (MQ=255)                                                                                                                                                                                                                                            ggcagAAATATCATTGGGTTCCAGCTGCGCCAATAAGTAGGAAAAATCCCGTCCCAGCTTACCCCAAGATGCCAATAAGGAATTACCCACCTGTAATTTTTCCTGCGCATAAGTCAGCTCCCAAt                                                                                                                                 >  1:138397/5‑125 (MQ=255)                                                                                                                                                                                                                                            ggcagAAATATCATTGGGTTCCAGCTGCGCCAATAAGTAGGAAAAATCCCGTCCCAGCTTACCCCAAGATGCCAATAAGGAATTACCCACCTGTAATTTTTCCTGCGCATAAGTCAGCTCCCAATGTTGCTGCGCCTGTGCAGCCTGTTCCGCCGATAATAAAGGCTTATCTGCGTGATTAAAATAATCGGTGCGAGTACGCAACGCCAGTTTTTCTACGAATTTCGGa                         <  1:112980/225‑1 (MQ=255)                                                                                                                                                                                                                                             gcagAAATATCATTGGGTTCCAGCTGCGCCAATAAGTAGGAAAAATCCCGTCCCAGCTTACCCCAAGATGCCAATAAGGAATTACCCACCTGTAATTTTTCCTGCGCATAAGTCAGCTCCCAATGTTGCTGCGCCTGTGCAGCCTGTTCCGCCGATAATAAAGGCTTATCTGCGTGATTAAAATAATCGGTGCGAGTACGCAACGCCAGTTTTTCTACGAATTTCGGATCAACGATGTCGCCCCAatat    <  1:472274/246‑1 (MQ=255)                                                                                                                                                                                                                                              cagAAATATCATTGGGTTCCAGCTGCGCCAATAAGTAGGAAAAATCCCGTCCCAGCTTACCCCAAGATGCCAATAAGGAAGTACCCACCTGTAATTTTTCCTGCGCATAAGTCAGCTCCCAATGTTGCTGCGCCTGTGCAGCCTGTTCCGCCGATAATAAAGGCTTATCTGCGTGATTAAAATAATCGGTGCGAGTACGCAACGCCAGTTTTTCTACGAATTTCGGATCAACGATGTCGCCCCAATATTc  <  1:430760/248‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                   ATATCCGCCACCATCACAATAATGTCTTTCGGCGTTAAATCAGGATTC‑TGTTGCAGCAAATGCAGCAAATAATCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAAGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGTGCCGTTCGGTGTTAAATGCAAAATACGATGTTGG‑ATCTGGCTTAATAAATTGTGCGGCATGATTTCCACATAGGCGGAAATGTCATTGGGTTCCAGCTGCGCCAATAAGTAGGAAAAATCCCGTCCCAGCTTACCCCAAGATGCCAATAAGGAATTACCCACCTGTAATTTTTCCTGCGCATAAGTCAGCTCCCAATGTTGCTGCGCCTGTGCAGCCTGTTCCGCCGATAATAAAGGCTTATCTGCGTGATTAAAATAATCGGTGCGAGTACGCAACGCCAGTTTTTCTACGAATTTCGGATCAACGATGTCGCCCCAATATTC  >  1/1832446‑1832930

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑