Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,832,665	A→G	intergenic (+50765/+2719)	unknown → / ← unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,832,665	0	A	G	100.0%	211.2 / NA	74	intergenic (+50765/+2719)	unknown/unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (34/40);  total (34/40)

CGGATATAGGGCGTGTATTTATCAATATCCGCCACCATCACAATAATGTCTTTCGGCGTTAAATCAGGATTC‑TGTTGCAGCAAATGCAGCAAATAATCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAAGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGTGCCGTTCGGTGTTAAATGCAAAATACGATGTTGG‑ATCTGGCTTAATAAATTGTGCGGCATGATTTCCACATAGGCGGAAATGTCATTGGGTTCCAGCTGCGCCAATAAGTAGGAAAAATCCCGTCCCAGCTTACCCCAAGATGCCAATAAGGAATTACCCACCTGTAATTTTTCCTGCGCATAAGTCAGCTCCCAATGTTGCTGCGCCTGTGCAGCCTGTTCCGCCGATAATAAAGGCTTATCTGCGTGATTAAAATAATCGGTGCGAGTACGCAACGCCAGTTTTTCTACGAATTTCGGATC  >  1/1832422‑1832909                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                     cgGATATACGGTGTGTATTTATCAATATCCGCCACCATCACAATAATGTCTTTCGGCGTTAAATC‑GGGTTCTTGTTGCAACAAATGTAGCAAATAATCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTc                                                                                                                                                                                                                                               <  1:601022/251‑1 (MQ=255)          ggtgtgtATTTATCAATATCCGCCACCATCACAATAATGTCTTTCGGCGTTAAATC‑GGGTTCTTGTTGCAACAAATGTAGCAAATAATCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCcac                                                                                                                                                                                                                                            >  1:681699/4‑245 (MQ=255)           gtgtgtATTTATCAATATCCGCCACCATCACAATAATGTCTTTCGGCGTTAAATC‑GGGTTCTTGTTGCAACAAATGTAGCAAATAATCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCcac                                                                                                                                                                                                                                            <  1:569963/242‑1 (MQ=255)           gtgtgtATTTATCAATATCCGCCACCATCACAATAATGTCTTTCGGCGTTAAATC‑GGGTTCTTGTTGCAACAAATGTAGCAAATAATCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGca                                                                                                                                                                                                                                     >  1:485779/3‑250 (MQ=255)             gtgtATTTATCAATATCCGCCACCATCACAATAATGTCTTTCGGCGTTAAATC‑GGGTTCTTGTTGCAACAAATGTAGCAAATAATCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGcaga                                                                                                                                                                                                                                   >  1:749093/1‑248 (MQ=255)             gtgtATTTATCAATATCCGCCACCATCACAATAATGTCTTTCGGCGTTAAATC‑GGGTTCTTGTTGCAACAAATGTAGCAAATAATCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGGTAAATGCAAAATACGGTGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGcaga                                                                                                                                                                                                                                   >  1:759311/1‑248 (MQ=255)                tATTTATCAATATCCGCCACCATCACAATAATGTCTTTCGGCGTTAAATC‑GGGTTCTTGTTGCAACAAATGTAGCAAATAATCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAAt                                                                                                                                                                                                                                <  1:115481/251‑1 (MQ=255)                  tttATCAATATCCGCCACCATCACAATAATGTCTTTCGGCGTTAAATC‑GGGTTCTTGTTGCAACAAATGTAGCAAATAATCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAAtat                                                                                                                                                                                                                              <  1:533578/251‑3 (MQ=255)                     aTCAATATCCGCCACCATCACAATAATGTCTTTCGGCGTTAAATC‑GGGTTCTTGTTGCAACAAATGTAGCAAATAATCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCcaca                                                                                                                                                                                                                                           >  1:834738/1‑235 (MQ=255)                         atatCCGCCACCATCACAATAATGTCTTTCGGCGTTAAATC‑GGGTTCTTGTTGCAACAAATGTAGCAAATAATCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTggg                                                                                                                                                                                                                       <  1:84990/251‑1 (MQ=255)                          tatCCGCCACCATCACAATAATGTCTTTCGGCGTTAAATC‑GGGTTCTTGTTGCAACAAATGTAGCAAATAATCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGGCATTCGGAGTTAAATGCAAAATACGGGGTTGG‑ATTTTGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTgggg                                                                                                                                                                                                                      >  1:516569/1‑250 (MQ=255)                                ccaccaTCACAATAATGTCTTTCGGCGTTAAATC‑GGGTTCTTGTTGCAACAAATGTAGCAAATAATCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGc                                                                                                                                                                                                                <  1:190776/251‑1 (MQ=255)                                ccaccaTCACAATAATGTCTTTCGGCGTTAAATC‑GGGTTCTTGTTGCAACAAATGTAGCAAATAATCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGc                                                                                                                                                                                                                <  1:191723/251‑1 (MQ=255)                                 caccaTCACAATAATGTCTTTCGGCGTTAAATC‑GGGTTCTTGTTGCAACAAATGTAGCAAATAATCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCt                                                                                                                                                                                                               <  1:168638/251‑1 (MQ=255)                                      tCACAATAATGTCTTTCGGCGTTAAATC‑GGGTTCTTGTTGCAACAAATGTAGCAAATAATCCCGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTgcgc                                                                                                                                                                                                           >  1:386103/1‑250 (MQ=255)                                         caATAATGTCTTTCGGCGTTAAATC‑GGGTTCTTGTTGCAACAAATGTAGCAAATAATCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGGGTTAAATGCAAAATACGGCGTTTG‑GTTTGGCCTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTGCGCCAAt                                                                                                                                                                                                       >  1:149453/1‑251 (MQ=255)                                         caATAATGTCTTTCGGCGTTAAATC‑GGGTTCTTGTTGCAACAAATGTAGCAAATAATCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTGCGCCAAt                                                                                                                                                                                                       >  1:583023/1‑251 (MQ=255)                                           ataatGTCTTTCGGCGTTAAATC‑GGGTTCTTGTTGCAACAAATGTAGCAAATAATCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTACCGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGG‑ATTTGGCCTAATAAATTGTGCGGCGGGATTTCCACATAGGCAGAAATATCATTGGGTTCCCGCTGCGCCCATaa                                                                                                                                                                                                     >  1:690580/1‑251 (MQ=255)                                             aatGTCTTTCGGCGTTAAATC‑GGGTTCTTGTTGCAACAAATGTAGCAAATAATCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTGCGCCAATAAGt                                                                                                                                                                                                   <  1:441935/251‑1 (MQ=255)                                                        gCGTTAAATC‑GGGTTCTTGTTGCAACAAATGTAGCAAATAATCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTGCGCCAATAAGTAGGAAAAATc                                                                                                                                                                                         <  1:773171/250‑1 (MQ=255)                                                             taaatcgggttcttGTTGCAACAAATGTAGCAAATAATCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTGCGCCAATAAGTAGGAAAAATCCCGTc                                                                                                                                                                                    <  1:691200/238‑1 (MQ=255)                                                               aatcgggttcttGTTGCAACAAATGTAGCAAATAATCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCa                                                                                                                                                                                                                            <  1:344305/198‑1 (MQ=255)                                                                     gttcttGTTGCAACAAATGTAGCAAATAATCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTGCGCCAATAAGTAGGAAAAATCCCGTCCCAGCtt                                                                                                                                                                             <  1:442252/245‑1 (MQ=255)                                                                                       gtagcaaatAATCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTGCGCCAATAAGTAGGaa                                                                                                                                                                                              <  1:402332/213‑1 (MQ=255)                                                                                            aaatAATCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTGCGCCAATAAGTAGGaaaa                                                                                                                                                                                            <  1:776337/212‑1 (MQ=255)                                                                                            aaatAATCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTGCGCCAATAAGTAGGAAAAATcc                                                                                                                                                                                        <  1:80016/216‑1 (MQ=255)                                                                                               taatCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTGCGCCAATAAGTAGGAAAAATCCCGTCCCAGCTTAcccc                                                                                                                                                                        <  1:379229/229‑1 (MQ=255)                                                                                                aatCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTGCGCCAATAAGTAGGAAAAATCCCGTCCCAGCTTACCCCAAGATGCCAATAAGGAATTAccc                                                                                                                                                 <  1:1874/251‑1 (MQ=255)                                                                                                aatCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTGCGCCAATAAGTAGGAAAAATCCCGTCCCAGCTTACCCCAAGATGCCAATAAGGAATTAccc                                                                                                                                                 <  1:900332/251‑1 (MQ=255)                                                                                                  tCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTGCGCCAATAAGTAGGAAAAATCCCGTCCCAGCTTACCCCAAGATGCCAATAAGGAATTAcc                                                                                                                                                  <  1:375139/248‑1 (MQ=255)                                                                                                         acacTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTGCGCCAATAAGTAGGAAAAATCCCGTCCCAGCTTACCCCAAGATGCCAATAAGGAATTACCCACCTGTAAt                                                                                                                                        <  1:42368/251‑1 (MQ=255)                                                                                                          cacttccacttcGCGCATCATGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTGCGCCAATAAGTAGGAAAAATCCCGTCCCAGCTTACCCCAAGATGCCAATAAGGAATTAccc                                                                                                                                                 <  1:80598/241‑1 (MQ=255)                                                                                                                         catcatGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTGCGCCAATAAGTAGGAAAAATCCCGTCCCAGCTTACCCCAAGATGCCAATAAGGAATTACCCACCTGTAATTTTTCCTGCGCATAAg                                                                                                                        >  1:470966/1‑251 (MQ=255)                                                                                                                          atcatGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCcac                                                                                                                                                                                                                                            <  1:214926/134‑1 (MQ=255)                                                                                                                           tcatGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTGCGCCAATAAGTAGGAAAAATCCCGTCCCAGCTTACCCCAAGATGCCAATAAGGAATTACCCACCTGTAATTTTTCCTGCGCATAAGTc                                                                                                                      <  1:626851/251‑1 (MQ=255)                                                                                                                           tcatGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTGCGCCAATAAGTAGGAAAAATCCCGTCCCAGCTTACCCCAAGATGCCAATAAGGAATTACCCACCTGTAATTTTTCCTGCGCATAAGTc                                                                                                                      <  1:729703/251‑1 (MQ=255)                                                                                                                             atGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGG‑ATTTGGCTTAATAAATTTTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTGCGCCAATAAGTAGGAAAAATCCCGTCCCAGCTTACCCCAAGATGCCAATAAGGAATTACCCACCTGTAATTTTTCCCGCGCATAAGTCAg                                                                                                                    >  1:502266/1‑251 (MQ=255)                                                                                                                             atGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTGCGCCAATAAGTAGGAAAAATCCCGTCCCAGCTTACCCCAAGATGCCAATAAGGAATTACCCACCTGTAATTTTTCCTGCGCATAAGTCAg                                                                                                                    >  1:551369/1‑251 (MQ=255)                                                                                                                                cTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTGCGCCAATAAGTAGGAAAAATCCCGTCCCAGCTTACCCCAAGa                                                                                                                                                                    >  1:501251/1‑200 (MQ=255)                                                                                                                                         gAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTGCGCCAATAAGTAGGAAAAATCCCGTCCCAGCTTACCCCAAGATGCCAATAAGGAATTACCCACCTGTAATTTTTCCTGCGCATAAGTCAGCTCCCAATGTtg                                                                                                        >  1:256013/1‑251 (MQ=255)                                                                                                                                          aaTGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTGCGCCAATAAGTAGGAAAAATCCCGTCCCAGCTTACCCCAAGATGCCAATAAGGAATTACCCACCTGTAATTTTTCCTGCGCATAAGTCAGCTCCCAATGTtgc                                                                                                       <  1:161007/251‑1 (MQ=255)                                                                                                                                          aaTGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTGCGCCAATAAGTAGGAAAAATCCCGTCCCAGCTTACCCCAAGATGCCAATAAGGAATTACCCACCTGTAATTTTTCCTGCGCATAAGTCAGCTCCCAATGTtgc                                                                                                       <  1:263270/251‑1 (MQ=255)                                                                                                                                                 ggTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTGCGCCAATAAGTAGGAAAAATCCCGTCCCAGCTTACCCCAAGATGCCAATAAGGAATTACCCACCTGTAATTTTTCCTGCGCATAAGTc                                                                                                                      <  1:530973/228‑1 (MQ=255)                                                                                                                                                  gTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTTCGCCAATAAGTAGGAAAAATCCCGTCCCAGCTTACCCCAAGATGCCAATAAGGAATTACCCACCTGTAATTTTTCCTGCGCATAAGGCAGCTCCCAATGTTGCTgcgc                                                                                                  >  1:241125/1‑248 (MQ=255)                                                                                                                                                     aaaGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTGCGCCAATAAGTAGGAAAAATCCCGTCCCAGCTTACCCCAAGATGCCAATAAGGAATTACCCACCTGTAATTTTTCCTGCGCATAAGTCAGCTCCCAATGTTGCTGCGCCTGTGc                                                                                            <  1:233331/251‑1 (MQ=255)                                                                                                                                                               cATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTGCGCCAATAAGTAGGAAAAATCCCGTCCCAGCTTACCCCAAGATGCCAATAAGGAATTACCCACCTGTAATTTTTCCTGCGCATAAGTCAGCTCCCAATGTTGCTGCGCCTGTGCAGCCTGTTc                                                                                   >  1:98117/1‑250 (MQ=255)                                                                                                                                                                    tCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGG‑ATTTGGCTTAATAAATTGGGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTGCGCCAATAAGTAGGAAAAATCCCGTCCCAGCTTACCCCAAGATGCCAATAAGGAATTACCCACCTGTAATTTTTCCTGCGCATAAGTCAGCTCCCAATGTTGCTGCGCCTGTGCAGCCTGTTCCGCCGa                                                                             >  1:316100/1‑251 (MQ=255)                                                                                                                                                                          tAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTGCGCCAATAAGTAGGAAAAATCCCGTCCCAGCTTACCCCAAGATGCCAATAAGGAATTACCCACCTGTAATTTTTCCTGCGCATAAGTCAGCTCCCAATGtt                                                                                                         <  1:514147/217‑1 (MQ=255)                                                                                                                                                                          tAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTGCGCCAATAAGTAGGAAAAATCCCGTCCCAGCTTACCCCAAGATGCCAATAAGGAATTACCCACCTGTAATTTTTCCTGCGCATAAGTCAGCTCCCAATGTTGCTGCGCCTGTGCAGCCTGTTCCGCCGAtaataa                                                                       >  1:510438/1‑251 (MQ=255)                                                                                                                                                                          tAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGGTGGAATTTGG‑TTAATAAATTGAGCGGCGTGATTTCCACAAAGGCAGAAATATCATTCGGTTTCAGCTGCGCCTTTAAGTAGGAAAAATCCCGTCCCAGCCTACCCCAAGATGCCAATAAGGAATTACCCACCTGTAATTTTTCCTGCGCATAAGTCAGCTCCCAATg                                                                                                           >  1:640273/1‑215 (MQ=255)                                                                                                                                                                            gTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTGCGCCAATAAGTAGGAAAAATCCCGTCCCAGCTTACCCCAAGATGCCAATAAGGAATTACCCACCTGTAATTTTTCCTGCGCATAAGTCAGCTCCCAATGTTGCTGCGCCTGTGCAGCCTGTTCCGCCGATAATAAAg                                                                     >  1:734751/1‑251 (MQ=255)                                                                                                                                                                            gTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTGCGCCAATAAGTAGGAAAAATCCCGTCCCAGCTTACCCCAAGATGCCAATAAGGAATTACCCACCTGTAATTTTTCCTGCGCATAAGTCAGCTCCCAATGTTGCTGCGCCTGTGCAGCCTGTTCCGCCGATAATAAAg                                                                     >  1:329665/1‑251 (MQ=255)                                                                                                                                                                            gTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTGCGCCAATAAGTAGGAAAAATCCCGTCCCAGCTTACCCCAAGATGCCAATAAGGAATTACCCACCTGTAATTTTTCCTGCGCATAAGTCAGCTCCCAATGTTGCTGCGCCTGTGCAGCCTGTTCCGCCGATAATAAAg                                                                     >  1:68301/1‑251 (MQ=255)                                                                                                                                                                                    gCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTGCGCCAATAAGTAGGAAAAATCCCGTCCCAGCTTACCCCAAGATGCCAATAAGGAATTACCCACCTGTAATTTTTCCTGCGCATAAGTCAGCTCCCAATGTTGCTGCGCCTGTGCAGCCTGTTCCGCCGATAATAAAg                                                                     <  1:42506/243‑1 (MQ=255)                                                                                                                                                                                    gCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTGCGCCAATAAGTAGGAAAAATCCCGTCCCAGCTTACCCCAAGATGCCAATAAGGAATTACCCACCTGTAATTTTTCCTGCGCATAAGTCAGCTCCCAATGTTGCTGCGCCTGTGCAGCCTGTTCCGCCGATAATAAAGGCTTATc                                                              <  1:66807/250‑1 (MQ=255)                                                                                                                                                                                    gCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTGCGCCAATAAGTAGGAAAAATCCCGTCCCAGCTTACCCCAAGATGCCAATAAGGAATTACCCACCTGTAATTTTTCCTGCGCATAAGTCAGCTCCCAATGTTGCTGCGCCTGTGCAGCCTGTTCCGCCGATAATAAAGGCTTATc                                                              >  1:346913/1‑250 (MQ=255)                                                                                                                                                                                    gCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTGCGCCAATAAGTAGGAAAAATCCCGTCCCAGCTTACCCCAAGATGCCAATAAGGAATTACCCACCTGTAATTTTTCCTGCGCATAAGTCAGCTCCCAATGTTGCTGCGCCTGTGCAGCCTGTTCCGCCGATAATAAAGGCTTATc                                                              <  1:548998/250‑1 (MQ=255)                                                                                                                                                                                     cGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTGCGCCAATAAGTAGGAAAAATCCCGTCCCAGCTTACCCCAAGATGCCAATAAGGAATTACCCACCTGTAATTTTTCCTGCGCATAAGTCAGCTCCCAATGTTGCTGCGCCTGTGCAGCCTGTTCCGCCGATAATAAAGGCTTATCTg                                                            <  1:126236/251‑1 (MQ=255)                                                                                                                                                                                             cattCGGAGTTAAATGCAAAATACGGTGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTGCGCCAATAAGTAGGAAAAATCCCGTCCCAGCTTACCCCAAGATGCCAATAAGGAATTACCCACCTGTAATTTTTCCTGCGCATAAGTCAGCTCCCAATGTTGCTGCGCCTGTGCAGCCTGTTCCGCCGATAATAAAGGCTTATCTGCGTGATTa                                                    <  1:404184/249‑1 (MQ=255)                                                                                                                                                                                              attCGGAGTTAAATGCAAAATACGGTGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTGCGCCAATAAGTAGGAAAAATCCCGTCCCAGCTTACCCCAAGATGCCAATAAGGAATTACCCACCTGTAATTTTTCCTGCGCATAAGTCAGCTCCCAATGTtgctg                                                                                                     >  1:489024/2‑201 (MQ=255)                                                                                                                                                                                              attCGGAGTTAAATGCAAAATACGGTGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTGCGCCAATAAGTAGGAAAAATCCCGTCCCAGCTTACCCCAAGATGCCAATAAGGAATTACCCACCTGTAATTTTTCCTGCGCATAAGTCAGCTCCCAATGTTGCTgcgc                                                                                                  >  1:290745/2‑204 (MQ=255)                                                                                                                                                                                                     gtTAAATGCAAAATACGGTGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTGCGCCAATAAGTAGGAAAAATCCCGTCCCAGCTTACCCCAAGATGCCAATAAGGAATTACCCACCTGTAATTTTTCCTGCGCATAAGTCAGCTCCCAATGTTGCTGCGCCTGTGCAGCCTGTTCCGCCGATAATAAAGGCTTATCTGCGTGATTAAaataat                                             >  1:876436/1‑250 (MQ=255)                                                                                                                                                                                                            gCAAAATACGGTGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTGCGCCAATAAGTAGGAAAAATCCCGTCCCAGCTTACCCCAAGATGCCAATAAGGAATTACCCACCTGTAATTTTTCCTGCGCATAAGTCAGCTCCCAATGTTGCTGCGCCTGTGCAGCCTGTTCCGCCGATAATAAAGGCTTATCTGCGTGATTAAAATAATCGGTGCGa                                     >  1:376/1‑251 (MQ=255)                                                                                                                                                                                                              aaaaTACGGTGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTGCGCCAATAAGTAGGAAAAATCCCGTCCCAGCTTACCCCAAGATGCCAATAAGGAATTACCCACCTGTAATTTTTCCTGCGCATAAGTCAGCTCCCAATGTTGCTGCGCCTGTGCAGCCTGTTCCGCCGATAATAAAGGCTTATCTGCGTGATTAAAATAATCGGTGCGAGt                                   >  1:729477/1‑251 (MQ=255)                                                                                                                                                                                                               aaaTACGGTGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTGCGCCAATAAGTAGGAAAAATCCCGTCCCAGCTTACCCCAAGATGCCAATAAGGAATTACCCACCTGTAATTTTTCCTGCGCATAAGTCAGCTCCCAATGTTGCTGCGCCTGTGCAGCCTGTTCCGCCGATAATAAAGGCTTATCTGCGTGATTAAAATAATCGGTGCGAGTa                                  <  1:283552/251‑1 (MQ=255)                                                                                                                                                                                                                aaTACGGTGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTGCGCCAATAAGTAGGAAAAATCCCGTCCCAGCTTACCCCAAGATGCCAATAAGGAATTACCCACCTGTAATTTTTCCTGCGCATAAGTCAGCTCCCAATGTTGCTGCGCCTGTGCAGCCTGTTCCGCCGATAATAAAGGCTTATCTGCGTGATTAAAATAATCGGTGCGAGTAc                                 <  1:841177/251‑1 (MQ=255)                                                                                                                                                                                                                 gtACGGTGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTGCGCCAATAAGTAGGAAAAATCCCGTCCCAGCTTACCCCAAGATGCCAATAAGGAATTACCCACCTGTAATTTTTCCTGCGCATAAGTCAGCTCCCAATGTTGCTGCGCCTGTGCAGCCTGTTCCGCCGATAATAAAGGCTTATCTGCGTGATTAAaat                                                >  1:510962/2‑235 (MQ=255)                                                                                                                                                                                                                      gtGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTGCGCCAATAAGTAGGAAAAATCCCGTCCCAGCTTACCCCAAGATGCCAATAAGGAATTACCCACCTGTAATTTTTCCTGCGCATAAGTc                                                                                                                      <  1:884644/159‑1 (MQ=255)                                                                                                                                                                                                                      gtGTTGG‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTGCGCCAATAAGTAGGAAAAATCCCGTCCCAGCTTACCCCAAGATGCCAATAAGGAATTACCCACCTGTAATTTTTCCTGCGCATAAGTCAGCTCCCAATGTTGCTGCGCCTGTGc                                                                                            >  1:894001/2‑186 (MQ=255)                                                                                                                                                                                                                           gg‑ATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTGCGCCAATAAGTAGGAAAAATCCCGTCCCAGCTTACCCCAAGATGCCAATAAGGAATTACCCACCTGTAATTTTTCCTGCGCATAAGTCAGCTCCCAATGTTGCTGCGCCTGTGCAGCCTGTTCCGCCGATAATAAAGGCTTATCTGCGTGATTAAAATAATCGGTGCGAGTACGCAACGCCAGt                      >  1:220176/1‑251 (MQ=255)                                                                                                                                                                                                                              atttGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATAGCATTGGGTTCCAGCTGCGCCAATAAGTAGGAAAAATCCCGTCCCAGCTTACCCCAAGATGCCAATAAGGAATTACCCACCTGTAATTTTTCCTGCGCATAAGTCAGCTCCCAATGTTGCTGCGCCTGTGCAGCCTGTTCCGCCGATAATAAAGGCTTATCTGCGTGATTAAAATAATCGGTGCGAGTACGCAACGCCAGtt                     <  1:763437/247‑1 (MQ=255)                                                                                                                                                                                                                                  ggCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTGCGCCAATAAGTAGGAAAAATCCCGTCCCAGCTTACCCCAAGATGCCAATAAGGAATTACCCACCTGTAATTTTTCCTGCGCATAAGTCAGCTCCCAATGTTGCTGCGCCTGTGCAGCCTGTTCCGCCGATAATAAAGGCTTATCTGCGTGATTAAAATAATCGGTGCGAGTACGCAACGCCAGttttt                  >  1:631672/1‑249 (MQ=255)                                                                                                                                                                                                                                       aataaATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTGCGCCAATAAGTAGGAAAAATCCCGTCCCAGCTTACCCCAAGATGCCAATAAGGAATTACCCACCTGTAATTTTTCCTGCGCATAAGTCAGCTCCCAATGTTGCTGCGCCTGTGCAGCCTGTTCCGCCGATAATAAAGGCTTATCTGCGTGATTAAAATAATCGGTGCGAGTACGCAACGCCAGTTTTTCTACGaa           >  1:98604/1‑251 (MQ=255)                                                                                                                                                                                                                                                tgCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTGCGCCAATAAGTAGGAAAAATCCCGTCCCAGCTTACCCCAAGATGCCAATAAGGAATTACCCACCTGTAATTTTTCCTGCGCATAAGTCAGCTCCCAATGTTGCTGCGCCTGTGCAGCCTGTTCCGCCGATAATAAAGGCTTATCTGCGTGATTAAAATAATCGGTGCGAGTACGCAACGCCAGTTTTTCTACGAATTTCGGATc  <  1:250919/251‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                     CGGATATAGGGCGTGTATTTATCAATATCCGCCACCATCACAATAATGTCTTTCGGCGTTAAATCAGGATTC‑TGTTGCAGCAAATGCAGCAAATAATCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAAGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGTGCCGTTCGGTGTTAAATGCAAAATACGATGTTGG‑ATCTGGCTTAATAAATTGTGCGGCATGATTTCCACATAGGCGGAAATGTCATTGGGTTCCAGCTGCGCCAATAAGTAGGAAAAATCCCGTCCCAGCTTACCCCAAGATGCCAATAAGGAATTACCCACCTGTAATTTTTCCTGCGCATAAGTCAGCTCCCAATGTTGCTGCGCCTGTGCAGCCTGTTCCGCCGATAATAAAGGCTTATCTGCGTGATTAAAATAATCGGTGCGAGTACGCAACGCCAGTTTTTCTACGAATTTCGGATC  >  1/1832422‑1832909

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑