Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,832,490	A→G	intergenic (+50590/+2894)	unknown → / ← unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,832,490	0	A	G	100.0%	205.2 / NA	70	intergenic (+50590/+2894)	unknown/unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/42);  total (28/42)

ACATCAAGCAATGCCAGCACGTCTTCGGCACTAAACGTGCTTTCTTTCAGCTGCAATAAGGTCAGAAAACCGGCGATAATCACATTATTCTCCGATAATTTGTTATCGGAAATGGAAAACGGAATAGCGGTCTGCGGCGTTGCATTTTGCCCGAACACGGCGCGGATATAGGGCGTGTATTTATCAATATCCGCCACCATCACAATAATGTCTTTCGGCGTTAAATCAGGATTC‑T‑GTTGCAGCAAATGCAGCAAATAATCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAAGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGTGCCGTTCGGTGTTAAATGCAAAATACGATGTTGGATCTGGCTTAATAAATTGTGCGGCATGATTTCCACATAGGCGGAAATGTCATTGGGTTCCAGCTGCGCCAATAAGTAGGAAAAATC  >  1/1832260‑1832726                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                               acaTCAAGCAATGCCAGCACGTCTTCTGCACTAAACGTGCTTTCTTTCAGCTGCAATAAGGTCAGAAAACCGGCGATAATCACGTTATTCTCCGATAATTTGTTATCGGAAATGGAAAACGGAATAGCGGTCTGCGGCGTTGCATTTTGCCCGAATACAGCGCGGATATACGGTGTGTATTTATCAATATCCGCCACCATCACAATAATGTCTTTCGGCGTTAAATC‑GGGTTCTT‑GTtgcaacaaatgtag                                                                                                                                                                                                                          <  1:41/251‑4 (MQ=255)           aTGCCAGCACGTCTTCTGCACTAAACGTGCTTTCTTTCAGCTGCGATAAGGTCAGAAAACCGGCGATAATCACGTTATTCTCCGATAATTTGTTATCGGAAATGGAAAACGGAATAGCGGTCTGCGGCGTTGCATTTTGCCCGAATACAGCGCGGATATACGGTGTGTATTTATCAATATCCGCCACCATCACAATAATGTCTTTCGGCGTTAAATC‑GGGTTCTT‑GTTGCAACAAATGTAGCAaataat                                                                                                                                                                                                                  >  1:290193/1‑249 (MQ=255)                 gCACGTCTTCTGCACTAAACGTGCTTTCTTTCAGCTGCAATAAGGTCAGAAAACCGGCGATAATCACGTTATTCTCCGATAATTTGTTATCGGAAATGGAAAACGGAATAGCGGTCTGCGGCGTTGCATTTTGCCCGAATACAGCGCGGATATACGGTGTGTATTTATCAATATCCGCCACCATCACAATAATGTCTTTCGGCGTTAAATC‑GGGTTCTT‑GTTgcaacaaatgtagcaaat                                                                                                                                                                                                                     >  1:604692/1‑240 (MQ=255)                  cACGTCTTCTGCACTAAACGTGCTTTCTTTCAGCTGCAATAAGGTCAGAAAACCGGCGATAATCACGTTATTCTCCGATAATTTGTTATCGGAAATGGAAAACGGAATAGCGGTCTGCGGCGTTGCATTTTGCCCGAATACAGCGCGGATATACGGTGTGTATTTATCAATATCCGCCACCATCACAATAATGTCTTTCGGCGTTAAATC‑GGGTTCTT‑GTTGCAACAAATGTAGCAAATAATCCTGCaa                                                                                                                                                                                                           <  1:396412/249‑1 (MQ=255)                       cTTCTGCACTAAACGTGCTTTCTTTCAGCTGCAATAAGGTCAGAAAACCGGCGATAATCACGTTATTCTCCGATAATTTGTTATCGGAAATGGAAAACGGAATAGCGGTCTGCGGCGTTGCATTTTGCCCGAATACAGCGCGGATATACGGTGTGTATTTATCAATATCCGCCACCATCACAATAATGTCTTTCGGCGTTAAATC‑GGGTTCTT‑GTTGCAACAAATGTAGCAAATAATCCTGCAAcacttc                                                                                                                                                                                                     >  1:724222/1‑250 (MQ=255)                         tctgCACTAAACGTGCTTTCTTTCAGCTGCAATAAGGTCAGAAAACCGGCGATAATCACGTTATTCTCCGATAATTTGTTATCGGAAATGGAAAACGGAATAGCGGTCTGCGGCGTTGCATTTTGCCCGAATACAGCGCGGATATACGGTGTGTATTTATCAATATCCGCCACCATCACAATAATGTCTTTCGGCGTTAAATC‑GGGTTCTT‑GTTGCAACAAATGTAGCAAATAATCCTGCAAcacttccac                                                                                                                                                                                                  <  1:326272/248‑1 (MQ=255)                           tgCACTAAACGTGCTTTCTTTCAGCTGCAATAAGGTCAGAAAACCGGCGATAATCACGTTATTCTCCGATAATTTGTTATCGGAAATGGAAAACGGAATAGCGGTCTGCGGCGTTGCATTTTGCCCGAATACAGCGCGGATATGCGGTGTGTATTTATCAATATCCGCCACCATCACAATAATGTCTTTCGGCGTTAAATC‑GGGTTCTT‑GTTGCAACAAATGTAGCAAATAATCCTGCAAcacttccactt                                                                                                                                                                                                <  1:34994/250‑1 (MQ=255)                                 aaaCGTGCTTTCTTTCAGCTGCAATAAGGTCAGAAAACCGGCGATAATCACGTTATTCTCCGATAATTTGTTATCGGAAATGGAAAACGGAATAGCGGTCTGCGGCGTTGCATTTTGCCCGAATACAGCGCGGATATACGGTGTGTATTTATCAATATCCGCCACCATCACAATAATGTCTTTCGGCGTTAAATC‑GGGTTCTT‑GTTGCAACAAATGTAGCAAATAATCCTGCAACACTTCCACTTCgcgc                                                                                                                                                                                           >  1:752696/1‑250 (MQ=255)                                        ctttctttCAGCTGCAATAAGGTCAGAAAACCGGCGATAATCACGTTATTCTCCGATAATTTGTTATCGGAAATGGAAAACGGAATAGCGGTCTGCGGCGTTGCATTTTGCCCGAATACAGCGCGGATATACGGTGTGTATTTATCAATATCCGCCACCATCACAATAATGTCTTTCGGCGTTAAATC‑GGGTTCTT‑GTTGCAACAAATGTAGCAAATAATCCTGCAACACTTCCACTTCGCGCATCATGCt                                                                                                                                                                                   <  1:347180/251‑1 (MQ=255)                                                  gCTGCAATAAGGTCAGAAAACCGGCGATAATCACGTTATTCTCCGATAATTTGTTATCGGAAATGGAAAACGGAATAGCGGTCTGCGGCGTTGCATTTTGCCCGAATACAGCGCGGATATACGGTGTGTATTTATCAATATCCGCCACCATCACAATAATGTCTTTCGGCGTTAAATC‑GGGTTCTT‑GTTGCAACAAATGTAGCAAATAATCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGaa                                                                                                                                                                         >  1:94105/1‑251 (MQ=255)                                                   cTGCAATAAGGTCAGAAAACCGGCGATAATCACGTTATTCTCCGATAATTTGTTATCGGAAATGGAAAACGGAATAGCGGTCTGCGGCGTTGCATTTTGCCCGAATACAGCGCGGATATACGGTGTGTATTTATCAATATCCGCCACCATCACAATAATGTCTTTCGGCGTTAAATC‑GGGTTCTT‑GTTGCAACAAATGTAGCAAATAATCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGa                                                                                                                                                                          <  1:316021/249‑1 (MQ=255)                                                     gCAATAAGGTCAGAAAACCGGCGATAATCACGTTATTCTCCGATAATTTGTTATCGGAAATGGAAAACGGAATAGCGGTCTGCGGCGTTGCATTTTGCCCGAATACAGCGCGGATATACGGTGTGTATTTATCAATATCCGCCACCATCACAATAATGTCTTTCGGCGTTAAATC‑GGGTTCTT‑GTTGCAACAAATGTAGCAAATAATCCTGCAACACTTCCACTTCGCGCATCATGCtgt                                                                                                                                                                                 <  1:448396/240‑1 (MQ=255)                                                            ggTCAGAAAACCGGCGATAATCACGTTATTCTCCGATAATTTGTTATCGGAAATGGAAAACGGAATAGCGGTCTGCGGCGTTGCATTTTGCCCGAATACAGCGCGGATATACGGTGTGTATTTATCAATATCCGCCACCATCACAATAATGTCTTTCGGCGTTAAATC‑GGGTTCTT‑GTTGCAACAAATGTAGCAAATAATCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAGGTTa                                                                                                                                                               >  1:876320/1‑251 (MQ=255)                                                                                       attCTCCGATAATTTGTTATCGGAAATGGAAAACGGAATAGCGGTCTGCGGCGTTGCATTTTGCCCGAATACAGCGCGGATATACGGTGTGTATTTATCAATATCCGCCACCATCACAATAATGTCTTTCGGCGTTAAATC‑GGGTTCTT‑GTTGCAACAAATGTAGCAAATAATCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTa                                                                                                                                    >  1:842447/1‑251 (MQ=255)                                                                                            ccGATAATTTGTTATCGGAAATGGAAAACGGAATAGCGGTCTGCGGCGTTGCATTTTGCCCGAATACAGCGCGGATATACGGTGTGTATTTATCAATATCCGCCACCATCACAATAATGTCTTTCGGCGTTAAATC‑GGGTTCTT‑GTTGCAACAAATGTAGCAAATAATCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAGGTTAAAGTg                                                                                                                                                          <  1:485002/224‑1 (MQ=255)                                                                                                   tttGTTATCGGAAATGGAAAACGGAATAGCGGTCTGCGGCGTTGCATTTTGCCCGAATACAGCGCGGATATACGGTGTGTATTTATCAATATCCGCCACCATCACAATAATGTCTTTCGGCGTTAAATC‑GGGTTCTT‑GTTGCAACAAATGTAGCAAATAATCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCgtggagc                                                                                                                        <  1:760579/251‑4 (MQ=255)                                                                                                   tttGTTATCGGAAATGGAAAACGGAATAGCGGTCTGCGGCGTTGCATTTTGCCCGAATACAGCGCGGATATACGGTGTGTATTTATCAATATCCGCCACCATCACAATAATGTCTTTCGGCGTTAAATC‑GGGTTCTT‑GTTGCAACAAATGTAGCAAATAATCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCgtggagc                                                                                                                        <  1:5076/251‑4 (MQ=255)                                                                                                   tttGTTATCGGAAATGGAAAACGGAATAGCGGTCTGCGGCGTTGCATTTTGCCCGAATACAGCGCGGATATACGGTGTGTATTTATCAATATCCGCCACCATCACAATAATGTCTTTCGGCGTTAAATC‑GGGTTCTT‑GTTGCAACAAATGTAGCAAATAATCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCgtggagc                                                                                                                        <  1:870268/251‑4 (MQ=255)                                                                                                             gAAATGGAAAACGGAATAGCGGTCTGCGGCGTTGCATTTTGCCCGAATACAGCGCGGATATACGGTGTGTATTTATCAATATCCGCCACCATCACAATAATGTCTTTCGGCGTTAAATC‑GGGTTCTT‑GTTGCAACAAATGTAGCAAATAATCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCggagt                                                                                                              >  1:601047/1‑248 (MQ=255)                                                                                                                           aaTAGCGGTCTGCGGCGTTGCATTTTGCCCGAATACAGCGCGGATATACGGTGTGTATTTATCAATATCCGCCACCATCACAATAATGTCTTTCGGCGTTAAATC‑GGGTTCTT‑GTTGCAACAAATGTAGCAAATAATCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATAc                                                                                                <  1:391933/251‑1 (MQ=255)                                                                                                                                  gTCTGCGGCGTTGCATTTTGCCCGAATACAGCGCGGATATACGGTGTGTATTTATCAATATCCGCCACCATCACAATAATGTCTTTCGGCGTTAAATC‑GGGTTCTT‑GTTGCAACAAATGTAGCAAATAATCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAAt                                                                                                         <  1:340081/235‑1 (MQ=255)                                                                                                                                     tGCGGCGTTGCATTTTGCCCGAATACAGCGCGGATATACGGTGTGTATTTATCAATATCCGCCACCATCACAATAATGTCTTTCGGCGTTAAATC‑GGGTTCTT‑GTTGCAACAAATGTAGCAAATAATCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGGAt                                                                                      <  1:392555/251‑1 (MQ=255)                                                                                                                                      gcggcgTTGCATTTTGCCCGAATACAGCGCGGATATACGGTGTGTATTTATCAATATCCGCCACCATCACAATAATGTCTTTCGGCGTTAAATC‑GGGTTCTT‑GTTGCAACAAATGTAGCAAATAATCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGGTACATGCAAAATACGGTGTTgggtt                                                                                     >  1:574647/1‑248 (MQ=255)                                                                                                                                         gcgTTGCATTTTGCCCGAATACAGCGCGGATATACGGTGTGTATTTATCAATATCCGCCACCATCACAATAATGTCTTTCGGCGTTAAATC‑GGGTTCTT‑GTTGCAACAAATGTAGCAAATAATCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAGGTTAAAGt                                                                                                                                                           <  1:140843/178‑1 (MQ=255)                                                                                                                                          cgTTGCATTTTGCCCGAATACAGCGCGGATATACGGTGTGTATTTATCAATATCCGCCACCATCACAATAATGTCTTTCGGCGTTAAATC‑GGGTTCTT‑GTTGCAACAAATGTAGCAAATAATCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGGATTTGGc                                                                                 <  1:258597/251‑1 (MQ=255)                                                                                                                                                aTTTTGCCCGAATACAGCGCGGATATACGGTGTGTATTTATCAATATCCGCCACCATCACAATAATGTCTTTCGGCGTTAAATC‑GGGTTCTT‑GTTGCAACAAATGTAGCAAATAATCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGGATTTGGCTtaata                                                                           <  1:811848/251‑1 (MQ=255)                                                                                                                                                   ttGCCCGAATACAGCGCGGATATACGGTGTGTATTTATCAATATCCGCCACCATCACAATAATGTCTTTCGGCGTTAAATC‑GGGTTCTT‑GTTGCAACAAATGTAGCAAATAATCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGGATTTGGCTTAATAAAt                                                                        <  1:207384/251‑1 (MQ=255)                                                                                                                                                     gCCCGAATACAGCGCGGATATACGGTGTGTATTTATCAATATCCGCCACCATCACAATAATGTCTTTCGGCGTTAAATC‑GGGTTCTT‑GTTGCAACAAATGTAGCAAATAATCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACggt                                                                                             <  1:393408/228‑3 (MQ=255)                                                                                                                                                     gCCCGAATACAGCGCGGATATACGGTGTGTATTTATCAATATCCGCCACCATCACAATAATGTCTTTCGGCGTTAAATC‑GGGTTCTT‑GTTGCAACAAATGTAGCAAATAATCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGGATTTTGCTTAATAAATtg                                                                      >  1:684701/1‑251 (MQ=255)                                                                                                                                                      cccGAATACAGCGCGGATATACGGTGTGTATTTATCAATATCCGCCACCATCACAATAATGTCTTTCGGCGTTAAATC‑GGGTTCTT‑GTTGCAACAAATGTAGCAAATAATCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGGATTTGGCTTAATAAATtg                                                                      <  1:531896/250‑1 (MQ=255)                                                                                                                                                          aatacagcgcGGATATACGGTGTGTATTTATCAATATCCGCCACCATCACAATAATGTCTTTCGGCGTTAAATC‑GGGTTCTT‑GTTGCAACAAATGTAGCAAATAATCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGGATTTGGCTTAATAAATTGTGCgg                                                                 <  1:192000/245‑1 (MQ=255)                                                                                                                                                          aatacagcgcGGATATACGGTGTGTATTTATCAATATCCGCCACCATCACAATAATGTCTTTCGGCGTTAAATC‑GGGTTC‑TCGTTGCAACAAATGTAGCAAATAATCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGGATTTGGCTTAATAAATTGTGCgg                                                                 <  1:450996/245‑1 (MQ=255)                                                                                                                                                           atacagcgcGGATATACGGTGTGTATTTATCAATATCCGCCACCATCACAATAATGTCTTTCGGCGTTAAATC‑GGGTTCTT‑GTTGCAACAAATGTAGCAAATAATCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGGATTTGGCTTAATAAATTGTGCggg                                                                >  1:44008/6‑250 (MQ=255)                                                                                                                                                           atacagcgcGGATATACGGTGTGTATTTATCAATATCCGCCACCATCACAATAATGTCTTTCGGCGTTAAATC‑GGGTTCTT‑GTTGCAACAAATGTAGCAAATAATCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAGGTTAAAGAGCGGTCATTTTCCCGATAGTTTAACgg                                                                                                                                >  1:403868/6‑187 (MQ=255)                                                                                                                                                                   cgGATATACGGTGTGTATTTATCAATATCCGCCACCATCACAATAATGTCTTTCGGCGTTAAATC‑GGGTTCTT‑GTTGCAACAAATGTAGCAAATAATCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGGATTTGGCTTAATAAATTGTGCGGCGTGATTTc                                                        <  1:601022/251‑1 (MQ=255)                                                                                                                                                                     gATATACGGTGTGTATTTATCAATATCCGCCACCATCACAATAATGTCTTTCGGCGTTAAATC‑GGGTTCTT‑GTtgcaacaaatgtagc                                                                                                                                                                                                                         <  1:700221/88‑1 (MQ=255)                                                                                                                                                                     gATATACGGTGTGTATTTATCAATATCCGCCACCATCACAATAATGTCTTTCGGCGTTAAATC‑GGGTTCTT‑GTTGCAACAAATGTAGCAAATAATCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAGGTTAAAg                                                                                                                                                            <  1:650588/149‑1 (MQ=255)                                                                                                                                                                            ggtgtgtATTTATCAATATCCGCCACCATCACAATAATGTCTTTCGGCGTTAAATC‑GGGTTCTT‑GTTGCAACAAATGTAGCAAATAATCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGGATTTGGCTTAATAAATTGTGCGGCGTGATTTCcac                                                     >  1:681699/4‑245 (MQ=255)                                                                                                                                                                             gtgtgtATTTATCAATATCCGCCACCATCACAATAATGTCTTTCGGTGTTAAATC‑GGGTTCTT‑GTTGCAACAAATGTAGCAAATAATCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGGGTTGGATTTGGc                                                                                 >  1:142643/3‑216 (MQ=255)                                                                                                                                                                             gtgtgtATTTATCAATATCCGCCACCATCACAATAATGTCTTTCGGCGTTAAATC‑GGGTTCTT‑GTTGCAACAAATGTAGCAAATAATCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGGAttt                                                                                    <  1:311179/211‑3 (MQ=255)                                                                                                                                                                             gtgtgtATTTATCAATATCCGCCACCATCACAATAATGTCTTTCGGCGTTAAATC‑GGGTTCTT‑GTTGCAACAAATGTAGCAAATAATCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGGAt                                                                                      <  1:346302/209‑1 (MQ=255)                                                                                                                                                                             gtgtgtATTTATCAATATCCGCCACCATCACAATAATGTCTTTCGGCGTTAAATC‑GGGTTCTT‑GTTGCAACAAATGTAGCAAATAATCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGGATTTGGCTTAATAAATTGTGc                                                                   >  1:640691/3‑230 (MQ=255)                                                                                                                                                                             gtgtgtATTTATCAATATCCGCCACCATCACAATAATGTCTTTCGGCGTTAAATC‑GGGTTCTT‑GTTGCAACAAATGTAGCAAATAATCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGGATTTGGCTTAATAAATTGTGCGGCGTGATTTCcac                                                     <  1:569963/242‑1 (MQ=255)                                                                                                                                                                             gtgtgtATTTATCAATATCCGCCACCATCACAATAATGTCTTTCGGCGTTAAATC‑GGGTTCTT‑GTTGCAACAAATGTAGCAAATAATCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGGATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGca                                              >  1:485779/3‑250 (MQ=255)                                                                                                                                                                               gtgtATTTATCAATATCCGCCACCATCACAATAATGTCTTTCGGCGTTAAATC‑GGGTTCTT‑GTTGCAACAAATGTAGCAAATAATCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGGATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGcaga                                            >  1:749093/1‑248 (MQ=255)                                                                                                                                                                               gtgtATTTATCAATATCCGCCACCATCACAATAATGTCTTTCGGCGTTAAATC‑GGGTTCTT‑GTTGCAACAAATGTAGCAAATAATCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGGTAAATGCAAAATACGGTGTTGGATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGcaga                                            >  1:759311/1‑248 (MQ=255)                                                                                                                                                                                 gtATTTATCAATATCCGCCACCATCACAATAATGTCTTTCGGCGTTAAATC‑GGGTTCTT‑GTTGCAACAAATGTAGCAAATAATCCTGCaa                                                                                                                                                                                                           >  1:810593/1‑90 (MQ=255)                                                                                                                                                                                  tATTTATCAATATCCGCCACCATCACAATAATGTCTTTCGGCGTTAAATC‑GGGTTCTT‑GTTGCAACAAATGTAGCAAATAATCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGGATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAAt                                         <  1:115481/251‑1 (MQ=255)                                                                                                                                                                                    tttATCAATATCCGCCACCATCACAATAATGTCTTTCGGCGTTAAATC‑GGGTTCTT‑GTTGCAACAAATGTAGCAAATAATCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGGATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAAtat                                       <  1:533578/251‑3 (MQ=255)                                                                                                                                                                                      tATCAATATCCGCCACCATCACAATAATGTCTTTCGGCGTTAAATC‑GGGTTCTT‑GTTGCAACAAATGTAGCAAATAATCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGGAt                                                                                      <  1:512690/202‑1 (MQ=255)                                                                                                                                                                                      tATCAATATCCGCCACCATCACAATAATGTCTTTCGGCGTTAAATC‑GGGTTCTT‑GTTGCAACAAATGTAGCAAATAATCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGGAt                                                                                      >  1:4492/1‑202 (MQ=255)                                                                                                                                                                                       aTCAATATCCGCCACCATCACAATAATGTCTTTCGGCGTTAAATC‑GGGTTCTT‑GTTGCAACAAATGTAGCAAATAATCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAAc                                                                                                                                  <  1:231023/157‑1 (MQ=255)                                                                                                                                                                                       aTCAATATCCGCCACCATCACAATAATGTCTTTCGGCGTTAAATC‑GGGTTCTT‑GTTGCAACAAATGTAGCAAATAATCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGGATTTGGCTTAATAAATTGTGCGGCGTGATTTCcaca                                                    >  1:834738/1‑235 (MQ=255)                                                                                                                                                                                           atatCCGCCACCATCACAATAATGTCTTTCGGCGTTAAATC‑GGGTTCTT‑GTTGCAACAAATGTAGCAAATAATCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGGATTTGGCTTAATAAAt                                                                        >  1:123916/1‑211 (MQ=255)                                                                                                                                                                                           atatCCGCCACCATCACAATAATGTCTTTCGGCGTTAAATC‑GGGTTCTT‑GTTGCAACAAATGTAGCAAATAATCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGGATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTggg                                <  1:84990/251‑1 (MQ=255)                                                                                                                                                                                            tatCCGCCACCATCACAATAATGTCTTTCGGCGTTAAATC‑GGGTTCTT‑GTTGCAACAAATGTAGCAAATAATCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGGCATTCGGAGTTAAATGCAAAATACGGGGTTGGATTTTGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTgggg                               >  1:516569/1‑250 (MQ=255)                                                                                                                                                                                                  ccaccaTCACAATAATGTCTTTCGGCGTTAAATC‑GGGTTCTT‑GTTGCAACAAATGTAGCAAATAATCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGGATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGc                         <  1:191723/251‑1 (MQ=255)                                                                                                                                                                                                  ccaccaTCACAATAATGTCTTTCGGCGTTAAATC‑GGGTTCTT‑GTTGCAACAAATGTAGCAAATAATCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGGATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGc                         <  1:190776/251‑1 (MQ=255)                                                                                                                                                                                                   caccaTCACAATAATGTCTTTCGGCGTTAAATC‑GGGTTCTT‑GTTGCAACAAATGTAGCAAATAATCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGGATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCt                        <  1:168638/251‑1 (MQ=255)                                                                                                                                                                                                     ccaTCACAATAATGTCTTTCGGCGTTAAATC‑GGGTTCTT‑GTTGCAACAAATGTAGCAAATAATCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGgtgt                                                                                           <  1:44975/182‑1 (MQ=255)                                                                                                                                                                                                        tCACAATAATGTCTTTCGGCGTTAAATC‑GGGTTCTT‑GTTGCAACAAATGTAGCAAATAATCCCGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGGATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTgcgc                    >  1:386103/1‑250 (MQ=255)                                                                                                                                                                                                         cacaATAATGTCTTTCGGCGTTAAATC‑GGGTTCTT‑GTTGCAACAAATGTAGCAAATAATCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTc                                                                                                                   <  1:287629/154‑1 (MQ=255)                                                                                                                                                                                                           caATAATGTCTTTCGGCGTTAAATC‑GGGTTCTT‑GTTGCAACAAATGTAGCAAATAATCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACgg                                                                                                                                <  1:636494/139‑1 (MQ=255)                                                                                                                                                                                                           caATAATGTCTTTCGGCGTTAAATC‑GGGTTCTT‑GTTGCAACAAATGTAGCAAATAATCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCgtggagc                                                                                                                        >  1:7048/1‑144 (MQ=255)                                                                                                                                                                                                           caATAATGTCTTTCGGCGTTAAATC‑GGGTTCTT‑GTTGCAACAAATGTAGCAAATAATCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGGGTTAAATGCAAAATACGGCGTTTGGTTTGGCCTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTGCGCCAAt                >  1:149453/1‑251 (MQ=255)                                                                                                                                                                                                           caATAATGTCTTTCGGCGTTAAATC‑GGGTTCTT‑GTTGCAACAAATGTAGCAAATAATCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGGATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTGCGCCAAt                >  1:583023/1‑251 (MQ=255)                                                                                                                                                                                                             ataatGTCTTTCGGCGTTAAATC‑GGGTTCTT‑GTTGCAACAAATGTAGCAAATAATCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTACCGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGGATTTGGCCTAATAAATTGTGCGGCGGGATTTCCACATAGGCAGAAATATCATTGGGTTCCCGCTGCGCCCATaa              >  1:690580/1‑251 (MQ=255)                                                                                                                                                                                                               aatGTCTTTCGGCGTTAAATC‑GGGTTCTT‑GTTGCAACAAATGTAGCAAATAATCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGGATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTGCGCCAATAAGt            <  1:441935/251‑1 (MQ=255)                                                                                                                                                                                                                atGTCTTTCGGCGTTAAATC‑GGGTTCTT‑GTTGCAACAAATGTAGCAAATAATCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGGATTTGGCTTAATAAAtt                                                                       <  1:586014/191‑1 (MQ=255)                                                                                                                                                                                                                          gCGTTAAATC‑GGGTTCTT‑GTTGCAACAAATGTAGCAAATAATCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAGGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGAGCCATTCGGAGTTAAATGCAAAATACGGTGTTGGATTTGGCTTAATAAATTGTGCGGCGTGATTTCCACATAGGCAGAAATATCATTGGGTTCCAGCTGCGCCAATAAGTAGGAAAAATc  <  1:773171/250‑1 (MQ=255)                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                               ACATCAAGCAATGCCAGCACGTCTTCGGCACTAAACGTGCTTTCTTTCAGCTGCAATAAGGTCAGAAAACCGGCGATAATCACATTATTCTCCGATAATTTGTTATCGGAAATGGAAAACGGAATAGCGGTCTGCGGCGTTGCATTTTGCCCGAACACGGCGCGGATATAGGGCGTGTATTTATCAATATCCGCCACCATCACAATAATGTCTTTCGGCGTTAAATCAGGATTC‑T‑GTTGCAGCAAATGCAGCAAATAATCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAAGTTAAAGTGCGGTCATTTTCCCGATAGTTTAACGGCGTGGTGCCGTTCGGTGTTAAATGCAAAATACGATGTTGGATCTGGCTTAATAAATTGTGCGGCATGATTTCCACATAGGCGGAAATGTCATTGGGTTCCAGCTGCGCCAATAAGTAGGAAAAATC  >  1/1832260‑1832726

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑