Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,832,343	A→G	intergenic (+50443/+3041)	unknown → / ← unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,832,343	0	A	G	100.0%	195.0 / NA	67	intergenic (+50443/+3041)	unknown/unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (36/31);  total (36/31)

ACCCAATAACATTCGCTCCAAGCCGGCTTGCCATGAATTGTAGTTACTTTCCGCCTCATCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCACGAATTTGCGCCAAATCCTGCAATTCAATATTAAAGCGTTGGCGAATTGCCGGCACATCAAGCAATGCCAGCACGTCTTCGGCACTAAACGTGCTTTCTTTCAGCTGCAATAAGGTCAGAAAACCGGCGATAATCACATTATTCTCCGATAATTTGTTATCGGAAATGGAAAACGGAATAGCGGTCTGCGGCGTTGCATTTTGCCCGAACACGGCGCGGATATAGGGCGTGTATTTATCAATATCCGCCACCATCACAATAATGTCTTTCGGCGTTAAATCAGGATTC‑TGTTGCAGCAAATGCAGCAAATAATCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAAGTTA  >  1/1832100‑1832569                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                    gcccAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAGCGTTGGCGAATTGCCGGCACATCAAGCAATGCCAGCACGTCTTCTGCACTAAACGTGCTTTCTTTCAGCTGCAATAAGGTCAGAAAACCGGCGATAATCACgttatt                                                                                                                                                                                                                                >  1:70469/2‑249 (MQ=255)  cccAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAGCGTTGGCGAATTGCCGGCACATCAAGCAATGCCAGCACGTCTTCTGCACTAAACGTGCTTTCTTTCAGCTGCAATAAGGTCAGAAAACCGGCGATAATCACGTTATtctc                                                                                                                                                                                                                             <  1:566190/251‑1 (MQ=255)     aacaaCATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAGCGTTGGCGAATTGCCGGCACATCAAGCAATGCCAGCACGTCTTCTGCACTAAACGTGCTTTCTTTCAGCTGCAATAAGGTCAGAAAACCGGCGATAATCACGTTATTCTCCGa                                                                                                                                                                                                                          <  1:257602/248‑1 (MQ=255)          cATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAGCGTTGGCGAATTGCCGGCACATCAAGCAATGCCAGCACGTCTTCTGCACTAAACGTGCTTTCTTTCAGCTGCAATAAGGTCAGAAAACCGGCGATAATCACGTTATTCTCCGATAAtt                                                                                                                                                                                                                     <  1:150683/251‑1 (MQ=255)                cTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAGCGTTGGCGAATTGCCGGCACATCAAGCAATGCCAGCACGTCTTCTGCACTAAACGTGCTTTCTTTCAGCTGCAATAAGGTCAGAAAACCGGCGATAATCACGTTATTCTCCGATAATTTGTTAt                                                                                                                                                                                                               >  1:829040/1‑251 (MQ=255)                    aaGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAGCGTTGGCGAATTGCCGGCACATCAAGCAATGCCAGCACGTCTTCTGCACTAAACGTGCTTTCTTTCAGCTGCAATAAGGTCAGAAAACCGGCGATAATCACGTTATTCTCCGATAAttt                                                                                                                                                                                                                    >  1:657960/1‑242 (MQ=255)                         cgcctGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAGCGTTGGCGAATTGCCGGCACATCAAGCAATGCCAGCACGTCTTCTGCACTAAACGTGCTTTCTTTCAGCTGCAATAAGGTCAGAAAACCGGCGATAATCACGTTATTCTCCGATAATTTGTTATCGGAAATgg                                                                                                                                                                                                      >  1:298457/5‑251 (MQ=255)                              gCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAGCGTTGGCGAATTGCCGGCACATCAAGCAATGCCAGCACGTCTTCTGCACTAAACGTGCTTTCTTTCAGCTGCAATAAGGTCAGAAAACCGGCGATAATCACGTTATTCTCCGATAATTTGTTATCGGAAATGGaaaa                                                                                                                                                                                                  >  1:483601/1‑250 (MQ=255)                              gCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAGCGTTGGCGAATTGCCGGCACATCAAGCAATGCCAGCACGCCTTCTGCACTAAACGTGCTTTCTTTCAGCTGCAATAAGGTCAGAAAACCGGCGATAATCACGTTATTCTCCGATAATTTGTTATCGGAAATGGaaaa                                                                                                                                                                                                  >  1:588525/1‑250 (MQ=255)                               ccATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAGCGTTGGCGAATTGCCGGCACATCAAGCAATGCCAGCACGTCTTCTGCACTAAACGTGCTTTCTTTCAGCTGCAATAAGGTCAGAAAACCGGCGATAATCACGTTATTCTCCGATAATTTGTTATCGGAAATGGAAAAc                                                                                                                                                                                                 <  1:888110/250‑1 (MQ=255)                                     aTTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAGCGTTGGCGAATTGCCGGCACATCAAGCAATGCCAGCACGTCTTCTGCACTAAACGTGCTTTCTTTCAGCTGCAATAAGGTCAGAAAACCGGCGATAATCACGTTATTCTCCGATAATTTGTTATCGGAAATGGAAAACGGAATAg                                                                                                                                                                                          <  1:616146/251‑1 (MQ=255)                                      ttGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAGCGTTGGCGAATTGCCGGCACATCAAGCAATGCCAGCACGTCTTCTGCACTAAACGTGCTTTCTTTCAGCTGCAATAAGGTCAGAAAACCGGCGATAATCACGTTATTCTCCGATAATTTGTTATCGGAAATGGAAAACGGAATAg                                                                                                                                                                                          >  1:502563/1‑250 (MQ=255)                                        gTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAGCGTTGGCGAATTGCCGGCACATCAAGCAATGCCAGCACGTCTTCTGCACTAAACGTGCTTTCTTTCAGCTGCAATAAGGTCAGAAAACCGGCGATAATCACGTTATTCTCCGATAATTTGTTATCGGAAATGGAAAACGGAATAGCgg                                                                                                                                                                                       >  1:171346/1‑251 (MQ=255)                                           gTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAGCGTTGGCGAATTGCCGGCACATCAAGCAATGCCAGCACGTCTTCTGCACTAAACGTGCTTTCTTTCAGCTGCAATAAGGTCAGAAAACCGGCGATAATCACGTTATTCTCCGATAATTTGTTATCGGAAATGGAAAACGGAATAGCGGt                                                                                                                                                                                      >  1:703082/1‑249 (MQ=255)                                               cTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAGCGTTGGCGAATTGCCGGCACATCAAGCAATGCCAGCACGTCTTCTGCACTAAACGTGCTTTCTTTCAGCTGCAATAAGGTCAGAAAACCGGCGATAATCACGTTATTCTCCGATAATTTGTTATCGGAAATGGAAAACGGAATAGCGGTCTgcgg                                                                                                                                                                                >  1:400498/1‑251 (MQ=255)                                                       ttcgtcatTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAGCGTTGGCGAATTGCCGGCACATCAAGCAATGCCAGCACGTCTTCTGCACTAAACGTGCTTTCTTTCAGCTGCAATAAGGTCAGAAAACCGGCGATAATCACGTTATTCTCCGATAATTTGTTATCGGAAATGGAAAACGGAATAGCGGTCTGCGGCGTTGCAt                                                                                                                                                                        <  1:14410/247‑1 (MQ=255)                                                         cgtcatTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAGCGTTGGCGAATTGCCGGCACATCAAGCAATGCCAGCACGTCTTCTGCACTAAACGTGCTTTCTTTCAGCTGCAATAAGGTCAGAAAACCGGCGATAATCACGTTATtctc                                                                                                                                                                                                                             >  1:748932/3‑196 (MQ=255)                                                             atTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAGCGTTGGCGAATTGCCGGCACATCAAGCAATGCCAGCACGTCTTCTGCACTAAACGTGCTTTCTTTCAGCTGCAATAAGGTCAGAAAACCGGCGATAATCACGTTATTCTCCGATAATTTGTTATCGGAAATGGAAAACGGAATAGCGGTCTGCGGCGTTGCATTTTGcc                                                                                                                                                                  <  1:85556/251‑1 (MQ=255)                                                                       gcattgcttgatgtgcCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAGCGTTGGCGAATTGCCGGCACATCAAGCAATGCCAGCACGTCTTCTGCACTAAACGTGCTTTCTTTCAGCTGCAATAAGGTCAGAAAACCGGCGATAATCACGTTATTCTCCGATAATTTGTTATCGGAAATGGAAAACGGAATAGCGGTCTGCGGCGTTGCATTTTGCCCGaatacag                                                                                                                                                         >  1:431129/15‑245 (MQ=255)                                                                         gCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAGCGTTGGCGAATTGCCGGCACATCAAGCAATGCCAGCACGTCTTCTGCACTAAACGTGCTTTCTTTCAGCTGCAATAAGGTCAGAAAACCGGCGATAATCACGTTATTCTCCGATAATTTGTTATCGGAAATGGAAAACGGAATAGCGGTCTGCGGCGTTGCATTTTGCCCGaatacagcg                                                                                                                                                       >  1:208279/1‑243 (MQ=255)                                                                         gCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAGCGTTGGCGAATTGCCGGCACATCAAGCAATGCCAGCACGTCTTCTGCACTAAACGTGCTTTCTTTCAGCTGCAATAAGGTCAGAAAACCGGCGATAATCACGTTATTCTCCGATAATTTGTTATCGGAAATGGAAAACGGAATAGCGGTCTGCGGCGTTGCATTTTGCCCGaatacagcg                                                                                                                                                       >  1:193113/1‑243 (MQ=255)                                                                         gCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAGCGTTGGCGAATTGCCGGCACATCAAGCAATGCCAGCACGTCTTCTGCACTAAACGTGCTTTCTTTCAGCTGCAATAAGGTCAGAAAACCGGCGATAATCACGTTATTCTCCGATAATTTGTTATCGGAAATGGAAAACGGAATAGCGGTCTGCGGCGTTGCATTTTGCCCGaatacagcg                                                                                                                                                       >  1:902070/1‑243 (MQ=255)                                                                                   acgcCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAGCGTTGGCGAATTGCCGGCACATCAAGCAATGCCAGCACGTCTTCTGCACTAAACGTGCTTTCTTTCAGCTGCAATAAGGTCAGAAAACCGGCGATAATCACGTTATTCTCCGATAATTTGTTATCGGAAATGGAAAACGGAATAGCGGTCTGCGGCGTTGCATTTTGCCCGAATACAGCGCGGAtatacgg                                                                                                                                            <  1:638236/251‑4 (MQ=255)                                                                                    cgcCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAGCGTTGGCGAATTGCCGGCATATCAAGCAATGCCAGCACGTCTTCTGCACTAAACGTGCTTTCTTTCAGCTGCAATAAGGTCAGAAAACCGGCGATAATCACGTTATTCTCCGATAATTTGTTATCGGAAATGGAAAACGGAATAGCGGTCTGCGGCGTTGCATTTTGCCCGAATACAGCGCGGAtatac                                                                                                                                              <  1:445536/248‑2 (MQ=255)                                                                                    cgcCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAGCGTTGGCGAATTGCCGGCACATCAAGCAATGCCAGCACGTCTTCTGCACTAAACGTGCTTTCTTTCAGCTGCAATAAGGTCAGAAAACCGGCGATAATCACGTTATTCTCCGATAATTTGTTATCGGAAATGGAAAACGGAATAGCGGTCTGCGGCGTTGCATTTTGCCCGAATACAGCGCGGAtatacggt                                                                                                                                           >  1:196346/1‑247 (MQ=255)                                                                                      ccGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAGCGTTGGCGAATTGCCGGCACATCAAGCAATGCCAGCACGTCTTCTGCACTAAACGTGCTTTCTTTCAGCTGCAATAAGGTCAGAAAACCGGCGATAATCACGTTATTCTCCGATAATTTGTTATCGGAAATGGAAAACGGAATAGCGGTCTGCGGCGTTGCATTTTGCCCGAATACAGCGCGGAtatacggtg                                                                                                                                          <  1:874733/250‑6 (MQ=255)                                                                                          cATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAGCGTTGGCGAATTGCCGGCACATCAAGCAATGCCAGCACGTCTTCTGCACTAAACGTGCTTTCTTTCAGCTGCAATAAGGTCAGAAAGCCGGCGATAATCACGTTATTCTCCGATAATTTGTTATCGGAAATGGAAAACGGAATAGCGGTCTGCGGCGTTGCATTTTGCCCGAATACAGCGCGGATATACGGTGTGTAt                                                                                                                                     <  1:377483/251‑1 (MQ=255)                                                                                          cATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAGCGTTGGCGAATTGCCGGCACATCAAGCAATGCCAGCACGTCTTCTGCACTAAACGTGCTTTCTTTCAGCTGCAATAAGGTCAGAAAACCGGCGATAATCACGTTATTCTCCGATAATTTGTTATCGGAAATGGAAAACGGAATAGCGGTCTGCGGCGTTGCATTTTGCCCGAATACAGCGCGGATATACGGTGTGTAt                                                                                                                                     <  1:32958/251‑1 (MQ=255)                                                                                             ttttCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAGCGTTGGCGAATTGCCGGCACATCAAGCAATGCCAGCACGTCTTCTGCACTAAACGTGCTTTCTTTCAGCTGCAATAAGGTCAGAAAACCGGCGATAATCACGTTATTCTCCGATAATTTGTTATCGGAAATGGAAAACGGAATAGCGGTCTGCGGCGTTGCATTTTGCCCGAATACAGCGCGGATATACGGTGTGTATTTa                                                                                                                                  >  1:382292/1‑251 (MQ=255)                                                                                             ttttCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAGCGTTGGCGAATTGCCGGCACATCAAGCAATGCCAGCACGTCTTCTGCACTAAACGTGCTTTCTTTCAGCTGCAATAAGGTCAGAAAACCGGCGATAATCACGTTATTCTCCGATAATTTGTTATCGGAAATGGAAAACGGAATAGCGGTCTGCGGCGTTGCATTTTGCCCGAATACAGCGCGGATATACGGTGTGTATTTa                                                                                                                                  <  1:461556/251‑1 (MQ=255)                                                                                               ttCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAGCGTTGGCGAATTGCCGGCACATCAAGCAATGCCAGCACGTCTTCTGCACTAAACGTGCTTTCTTTCAGCTGCAATAAGGTCAGAAAACCGGCGATAATCACGTTATTCTCCGATAATTTGTTATCGGAAATGGAAAACGGAATAGCGGTCTGCGGCGTTGCATTTTGCCCGAATACAGCGCGGATATACGGTGTGTATTTATc                                                                                                                                <  1:455299/251‑1 (MQ=255)                                                                                               ttCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAGCGTTGGCGAATTGCCGGCACATCAAGCAATGACAGCACGTCTTCTGCACTAAACGTGCTTTCTTTCAGCTGCAATAAGGTCAGAAAACCGGCGATAATCACGTTATTCTCCGATAATTTGTTATCGGAAATGGAAAACGGAATAGCGGTCTGCGGCGTTGCATTTTGCCCGAATACAGCGCGGATATACGGTGTGTATTGATc                                                                                                                                <  1:455259/251‑1 (MQ=255)                                                                                                tCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAGCGTTGGCGAATTGCCGGCACATCAAGCAATGCCAGCACGTCTTCTGCACTAAACGTGCTTTCTTTCAGCTGCAATAAGGTCAGAAAACCGGCGATAATCACGTTATTCTCCGATAATTTGTTATCGGAAATGGAAAACGGAATAGCGGTCTGCGGCGTTGCATTTTGCCCGAATACAGCGCGGATATACGGTGTGTATTTATCa                                                                                                                               <  1:219851/251‑1 (MQ=255)                                                                                                         cgcGAATTTGCGCCAAATCCTGCAATTCAATATTAAAGCGTTGGCGAATTGCCGGCACATCAAGCAATGCCAGCACGTCTTCTGCACTAAACGTGCTTTCTTTCAGCTGCAATAAGGTCAGAAAACCGGCGATAATCACGTTATTCTCCGATAATTTGTTATCGGAAATGGAAAACGGAATAGCGGTCTGCGGCGTTGCATTTTGCCCGAATACAGCGCGGATATACGGTGTGTATTTATCAATATCCGcc                                                                                                                      <  1:197459/249‑1 (MQ=255)                                                                                                                   cgcCAAATCCTGCAATTCAATATTAAAGCGTTGGCGAATTGCCGGCACATCAAGCAATGCCAGCACGTCTTCTGCACTAAACGTGCTTTCTTTCAGCTGCAATAAGGTCAGAAAACCGGCGATAATCACGTTATTCTCCGATAATTTGTTATCGGAAATGGAAAACGGAATAGCGGTCTGCGGCGTTGCATTTTGCCCGAATACAGCGCGGATATACGGTGTGTATTTATCAATATCCGCCACCATCACaa                                                                                                            >  1:882144/1‑251 (MQ=255)                                                                                                                    gcCAAATCCTGCAATTCAATATTAAAGCGTTGGCGAATTGCCGGCACATCAAGCAATGCCAGCACGTCTTCTGCACTAAACGTGCTTTCTTTCAGCTGCAATAAGGTCAGAAAACCGGCGATAATCACGTTATTCTCCGATAATTTGTTATCGGAAATGGAAAACGGAATAGCGGTCTGCGGCGTTGCATTTTGCCCGAATACAGCGCGGATATACGGTGTGTATTTATCAATATCCGCCACCATCACaat                                                                                                           >  1:421905/1‑251 (MQ=255)                                                                                                                     ccAAATCCTGCAATTCAATATTAAAGCGTTGGCGAATTGCCGGCACATCAAGCAATGCCAGCACGTCTTCTGCACTAAACGTGCTTTCTTTCAGCTGCAATAAGGTCAGAAAACCGGCGATAATCACGTTATTCTCCGATAATTTGTTATCGGAAATGGAAAACGGAATAGCGGTCTGCGGCGTTGCATTTTGCCCGAATACAGCGCGGATATACGGTGTGTATTTATCAATATCCGCCACCATCACaat                                                                                                           <  1:829793/250‑1 (MQ=255)                                                                                                                         aTCCTGCAATTCAATATTAAAGCGTTGGCGAATTGCCGGCACATCAAGCAATGCCAGCACGTCTTCTGCACTAAACGTGCTTTCTTTCAGCTGCAATAAGGTCAGAAAACCGGCGATAATCACGTTATTCTCCGATAATTTGTTATCGGAAATGGAAAACGGAATAGCGGTCTGCGGCGTTGCATTTTGCCCGAATACAGCGCGGATATACGGTGTGTATTTATCAATATCCGCCACCATCACAATAATGt                                                                                                      <  1:579510/251‑1 (MQ=255)                                                                                                                          tCCTGCAATTCAATATTAAAGCGTTGGCGAATTGCCGGCACATCAAGCAATGCCAGCACGTCTTCTGCACTAAACGTGCTTTCTTTCAGCTGCAATAAGGTCAGAAAACCGGCGATAATCACGTTATTCTCCGATAATTTGTTATCGGAAATGGAAAACGGAATAGCGGTCTGCGGCGTTGCATTTTGCCCGAATACAGCGCGGAtatacgg                                                                                                                                            <  1:253468/212‑4 (MQ=255)                                                                                                                               cAATTCAATATTAAAGCGTTGGCGAATTGCCGGCACATCAAGCAATGCCAGCACGTCTTCTGCACTAAACGTGCTTTCTTTCAGCTGCAATAAGGTCAGAAAACCGGCGATAATCACGTTATTCTCCGATAATTTGTTATCGGAAATGGAAAACGGAATAGCGGTCTGCGGCGTTGCATTTTGCCCGAATACAGCGCGGATATACGGTGTGTATTTATCAATATCCGCCACCATCACAATAATGTCTTTCg                                                                                                >  1:482480/1‑251 (MQ=255)                                                                                                                                 aTTCAATATTAAAGCGTTGGCGAATTGCCGGCACATCAAGCAATGCCAGCACGTCTTCTGCACTAAACGTGCTTTCTTTCAGCTGCAATAAGGTCAGAAAACCGGCGATAATCACGTTATTCTCCGATAATTTGTTATCGGAAATGGAAAACGGAATAGCGGTCTGCGGCGTTGCATTTTGCCCGAATACAGCGCGGATATACGGTGTGTATTTATCAATATCCGCCACCATCACAATAATGTCTTTCgg                                                                                               >  1:737797/1‑250 (MQ=255)                                                                                                                                    cAATATTAAAGCGTTGGCGAATTGCCGGCACATCAAGCAATGCCAGCACGTCTTCTGCACTAAACGTGCTTTCTTTCAGCTGCAATAAGGTCAGAAAACCGGCGATAATCACGTTATTCTCCGATAATTTGTTATCGGAAATGGAAAACGGAATAGCGGTCTGCGGCGTTGCATTTTGCCCGAATACAGCGCGGATATACGGTGTGTATTTATCAATATCCGCCACCATCACAATAATGTCTTTCGGCGtt                                                                                           >  1:507539/1‑251 (MQ=255)                                                                                                                                      atatTAAAGCGTTGGCGAATTGCCGGCACATCAAGCAATGCCAGCACGTCTTCTGCACTAAACGTGCTTTCTTTCAGCTGCAATAAGGTCAGAAAACCGGCGATAATCACGTTATTCTCCGATAATTTGTTATCGGAAATGGAAAACGGAATAGCGGTCTGCGGCGTTGCATTTTGCCCGAATACAGCGCGGATATACGGTGTGTATTTATCAATATCCGCCACCATCACAATAATGTCTTTCGGCGTTaa                                                                                         >  1:485854/1‑251 (MQ=255)                                                                                                                                      atatTAAAGCGTTGGCGAATTGCCGGCACATCAAGCAATGCCAGCACGTCTTCTGCACTAAACGTGCTTTCTTTCAGCTGCAATAAGGTCAGAAAACCGGCGATAATCACGTTATTCTCCGATAATTTGTTATCGGAAATGGAAAACGGAATAGCGGTCTGCGGCGTTGCATTTTGCCCGAATACAGCGCGGATATACGGTGTGTATTTATCAATATCCGCCACCATCACAATAATGTCTTTCGGCGTTaa                                                                                         >  1:35616/1‑251 (MQ=255)                                                                                                                                      atatTAAAGCGTTGGCGAATTGCCGGCACATCAAGCAATGCCAGCACGTCTTCTGCACTAAACGTGCTTTCTTTCAGCTGCAATAAGGTCAGAAAACCGGCGATAATCACGTTATTCTCCGATAATTTGTTATCGGAAATGGAAAACGGAATAGCGGTCTGCGGCGTTGCATTTTGCCCGAATACAGCGCGGATATACGGTGTGTATTTATCAATATCCGCCACCATCACAATAATGTCTTTCGGCGTTaa                                                                                         >  1:390083/1‑251 (MQ=255)                                                                                                                                        atTAAAGCGTTGGCGAATTGCCGGCACATCAAGCAATGCCAGCACGTCTTCTGCACTAAACGTGCTTTCTTTCAGCTGCAATAAGGTCAGAAAACCGGCGATAATCACGTTATTCTCCGATAATTTGTTATCGGAAATGGAAAACGGAATAGCGGTCTGCGGCGTTGCATTTTGCCCGaata                                                                                                                                                            <  1:278961/182‑3 (MQ=255)                                                                                                                                        atTAAAGCGTTGGCGAATTGCCGGCACATCAAGCAATGCCAGCACGTCTTCTGCACTAAACGTGCTTTCTTTCAGCTGCAATAAGGTCAGAAAACCGGCGATAATCACGTTATTCTCCGATAATTTGTTATCGGAAATGGAAAACGGAATAGCGGTCTGCGGCGTTGCATTTTGCCCGAATACAGCGCGGATATACGGTGTGTATTTATCAATATCCGCCACCATCACAATAATGTCt                                                                                                    >  1:855997/1‑238 (MQ=255)                                                                                                                                                   ggCGAATTGCCGGCACATCAAGCAATGCCAGCACGTCTTCTGCACTAAACGTGCTTTCTTTCAGCTGCAATAAGGTCAGAAAACCGGCGATAATCACGTTATTCTCCGATAATTTGTTATCGGAAATGGAAAACGGAATAGCGGTCTGCGGCGTTGCATTTTGCCCGAATACAGCGCGGAtatacgg                                                                                                                                            >  1:14999/1‑184 (MQ=255)                                                                                                                                                         ttGCCGGCACATCAAGCAATGCCAGCACGTCTTCTGCACTAAACGTGCTTTCTTTCAGCTGCAATAAGGTCAGAAAACCGGCGATAATCACGTTATTCTCCGATAATTTGTTATCGGAAATGGAAAACGGAATAGCGGTCTGCGGCGTTGCATTTTGCCCGAATACAGCGCGGATATACGGTGTGTATTTATc                                                                                                                                <  1:8595/193‑1 (MQ=255)                                                                                                                                                           gCCGGCACATCAAGCAATGCCAGCACGTCTTCTGCACTAAACGTGCTTTCTTTCAGCTGCAATAAGGTCAGAAAACCGGCGATAATCACGTTATTCTCCGATAATTTGTTATCGGAAATGGAAAACGGAATAGCGGTCTGCGGCGTTGCATTTTGCCCGAATACAGCGCGGATATACGGTGTGTATTTATCAATATCCGCCACCATCACAATAATGTCTTTCGGc                                                                                              >  1:81253/1‑225 (MQ=255)                                                                                                                                                                 acaTCAAGCAATGCCAGCACGTCTTCTGCACTAAACGTGCTTTCTTTCAGCTGCAATAAGGTCAGAAAACCGGCGATAATCACGTTATTCTCCGATAATTTGTTATCGGAAATGGAAAACGGAATAGCGGTCTGCGGCGTTGCATTTTGCCCGAATACAGCGCGGATATACGGTGTGTATTTATCAATATCCGCCACCATCACAATAATGTCTTTCGGCGTTAAATC‑GGGTTCTTGTtgcaacaaatgtag                                                             <  1:41/251‑4 (MQ=255)                                                                                                                                                                       aGCAATGCCAGCACGTCTTCTGCACTAAACGTGCTTTCTTTCAGCTGCAATAAGGTCAGAAAACCGGCGATAATCACGTTATTCTCCGATAATTTGTTATCGGAAATGGAAAACGGAATAGCGGTCTGCGGCGTTGCATTTTGCCCGAATACAGCGCGGATATACGGTGTGTATTTATCAATATCCGCCACCATCACAATAATGTCTTTCGGCGTTAAATcgggttcttgttgcaacaa                                                                    >  1:595041/1‑221 (MQ=255)                                                                                                                                                                           aTGCCAGCACGTCTTCTGCACTAAACGTGCTTTCTTTCAGCTGCGATAAGGTCAGAAAACCGGCGATAATCACGTTATTCTCCGATAATTTGTTATCGGAAATGGAAAACGGAATAGCGGTCTGCGGCGTTGCATTTTGCCCGAATACAGCGCGGATATACGGTGTGTATTTATCAATATCCGCCACCATCACAATAATGTCTTTCGGCGTTAAATC‑GGGTTCTTGTTGCAACAAATGTAGCAaataat                                                     >  1:290193/1‑249 (MQ=255)                                                                                                                                                                                 gCACGTCTTCTGCACTAAACGTGCTTTCTTTCAGCTGCAATAAGGTCAGAAAACCGGCGATAATCACGTTATTCTCCGATAATTTGTTATCGGAAATGGAAAACGGAATAGCGGTCTGCGGCGTTGCATTTTGCCCGAATACAGCGCGGATATACGGTGTGTATTTATCAATATCCGCCACCATCACAATAATGTCTTTCGGCGTTAAATC‑GGGTTCTTGTTgcaacaaatgtagcaaat                                                        >  1:604692/1‑240 (MQ=255)                                                                                                                                                                                  cACGTCTTCTGCACTAAACGTGCTTTCTTTCAGCTGCAATAAGGTCAGAAAACCGGCGATAATCACGTTATTCTCCGATaa                                                                                                                                                                                                                       >  1:727412/1‑81 (MQ=255)                                                                                                                                                                                  cACGTCTTCTGCACTAAACGTGCTTTCTTTCAGCTGCAATAAGGTCAGAAAACCGGCGATAATCACGTTATTCTCCGATAATTTGTTATCGGAAATGGAAAACGGAATAGCGGTCTGCGGCGTTGCATTTTGCCCGAATACAGCGCGGATATACGGTGTGTATTTATCAATATCCGCCACCATCACAATAATGTCTTTCGGCGTTAAATC‑GGGTTCTTGTTGCAACAAATGTAGCAAATAATCCTGCaa                                              <  1:396412/249‑1 (MQ=255)                                                                                                                                                                                     gTCTTCTGCACTAAACGTGCTTTCTTTCAGCTGCAATAAGGTCAGAAAACCGGCGATAATCACGTTATTCTCCGATAATTTGTTATCGGAAATGGAAAACGGAATAGCGGTCTGCGGCGTTGCATTTTGCCCGAATACAGCGCGGAtatacgg                                                                                                                                            <  1:184433/153‑4 (MQ=255)                                                                                                                                                                                       cTTCTGCACTAAACGTGCTTTCTTTCAGCTGCAATAAGGTCAGAAAACCGGCGATAATCACGTTATTCTCCGATAATTTGTTATCGGAAATGGAAAACGGAATAGCGGTCTGCGGCGTTGCATTTTGCCCGAATACAGCGCGGATATACGGTGTGTATTTATCAATATCCGCCACCATCACAATAATGTCTTTCGGCGTTAAATC‑GGGTTCTTGTTGCAACAAATGTAGCAAATAATCCTGCAAcacttc                                        >  1:724222/1‑250 (MQ=255)                                                                                                                                                                                         tctgCACTAAACGTGCTTTCTTTCAGCTGCAATAAGGTCAGAAAACCGGCGATAATCACGTTATTCTCCGATAATTTGTTATCGGAAATGGAAAACGGAATAGCGGTCTGCGGCGTTGCATTTTGCCCGAATACAGCGCGGATATACGGTGTGTATTTATCAATATCCGCCACCATCACAATAATGTCTTTCGGCGTTAAATC‑GGGTTCTTGTTGCAACAAATGTAGCAAATAATCCTGCAAcacttccac                                     <  1:326272/248‑1 (MQ=255)                                                                                                                                                                                           tgCACTAAACGTGCTTTCTTTCAGCTGCAATAAGGTCAGAAAACCGGCGATAATCACGTTATTCTCCGATAATTTGTTATCGGAAATGGAAAACGGAATAGCGGTCTGCGGCGTTGCATTTTGCCCGAATACAGCGCGGATATGCGGTGTGTATTTATCAATATCCGCCACCATCACAATAATGTCTTTCGGCGTTAAATC‑GGGTTCTTGTTGCAACAAATGTAGCAAATAATCCTGCAAcacttccactt                                   <  1:34994/250‑1 (MQ=255)                                                                                                                                                                                            gCACTAAACGTGCTTTCTTTCAGCTGCAATAAGGTCAGAAAACCGGCGATAATCACGTTATTCTCCGATAATTTGTTATCGGAAATGGAAAACGGAATAGCGGTCTGCGGCGTTGCATTTTGCCCGAATACAGCGCGGATATACGGTGTGTATTTATCAATATCCGCCACCATCACAATAATGTCTTTCgg                                                                                               >  1:635532/1‑191 (MQ=255)                                                                                                                                                                                                 aaaCGTGCTTTCTTTCAGCTGCAATAAGGTCAGAAAACCGGCGATAATCACGTTATTCTCCGATAATTTGTTATCGGAAATGGAAAACGGAATAGCGGTCTGCGGCGTTGCATTTTGCCCGAATACAGCGCGGATATACGGTGTGTATTTATCAATATCCGCCACCATCACAATAATGTCTTTCGGCGTTAAATC‑GGGTTCTTGTTGCAACAAATGTAGCAAATAATCCTGCAACACTTCCACTTCgcgc                              >  1:752696/1‑250 (MQ=255)                                                                                                                                                                                                        ctttctttCAGCTGCAATAAGGTCAGAAAACCGGCGATAATCACGTTATTCTCCGATAATTTGTTATCGGAAATGGAAAACGGAATAGCGGTCTGCGGCGTTGCATTTTGCCCGAATACAGCGCGGATATACGGTGTGTATTTATCAATATCCGCCACCATCACAATAATGTCTTTCGGCGTTAAATC‑GGGTTCTTGTTGCAACAAATGTAGCAAATAATCCTGCAACACTTCCACTTCGCGCATCATGCt                      <  1:347180/251‑1 (MQ=255)                                                                                                                                                                                                           tctttcAGCTGCAATAAGGTCAGAAAACCGGCGATAATCACGTTATTCTCCGATAATTTGTTATCGGAAATGGAAAACGGAATAGCGGTCTGCGGCGTTGCATTTTGCCCGAATACAGCGCGGATATACGGTGTGTATTTATCAATATCCGCCACCATCACaa                                                                                                            <  1:571137/163‑1 (MQ=255)                                                                                                                                                                                                                  gCTGCAATAAGGTCAGAAAACCGGCGATAATCACGTTATTCTCCGATAATTTGTTATCGGAAATGGAAAACGGAATAGCGGTCTGCGGCGTTGCATTTTGCCCGAATACAGCGCGGATATACGGTGTGTATTTATCAATATCCGCCACCATCACAATAATGTCTTTCGGCGTTAAATC‑GGGTTCTTGTTGCAACAAATGTAGCAAATAATCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGaa            >  1:94105/1‑251 (MQ=255)                                                                                                                                                                                                                   cTGCAATAAGGTCAGAAAACCGGCGATAATCACGTTATTCTCCGATAATTTGTTATCGGAAATGGAAAACGGAATAGCGGTCTGCGGCGTTGCATTTTGCCCGAATACAGCGCGGATATACGGTGTGTATTTATCAATATCCGCCACCATCACAATAATGTCTTTCGGCGTTAAATC‑GGGTTCTTGTTGCAACAAATGTAGCAAATAATCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGa             <  1:316021/249‑1 (MQ=255)                                                                                                                                                                                                                     gCAATAAGGTCAGAAAACCGGCGATAATCACGTTATTCTCCGATAATTTGTTATCGGAAATGGAAAACGGAATAGCGGTCTGCGGCGTTGCATTTTGCCCGAATACAGCGCGGATATACGGTGTGTATTTATCAATATCCGCCACCATCACAATAATGTCTTTCGGCGTTAAATC‑GGGTTCTTGTTGCAACAAATGTAGCAAATAATCCTGCAACACTTCCACTTCGCGCATCATGCtgt                    <  1:448396/240‑1 (MQ=255)                                                                                                                                                                                                                            ggTCAGAAAACCGGCGATAATCACGTTATTCTCCGATAATTTGTTATCGGAAATGGAAAACGGAATAGCGGTCTGCGGCGTTGCATTTTGCCCGAATACAGCGCGGATATACGGTGTGTATTTATCAATATCCGCCACCATCACAATAATGTCTTTCGGCGTTAAATC‑GGGTTCTTGTTGCAACAAATGTAGCAAATAATCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAGGTTa  >  1:876320/1‑251 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                    ACCCAATAACATTCGCTCCAAGCCGGCTTGCCATGAATTGTAGTTACTTTCCGCCTCATCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCACGAATTTGCGCCAAATCCTGCAATTCAATATTAAAGCGTTGGCGAATTGCCGGCACATCAAGCAATGCCAGCACGTCTTCGGCACTAAACGTGCTTTCTTTCAGCTGCAATAAGGTCAGAAAACCGGCGATAATCACATTATTCTCCGATAATTTGTTATCGGAAATGGAAAACGGAATAGCGGTCTGCGGCGTTGCATTTTGCCCGAACACGGCGCGGATATAGGGCGTGTATTTATCAATATCCGCCACCATCACAATAATGTCTTTCGGCGTTAAATCAGGATTC‑TGTTGCAGCAAATGCAGCAAATAATCCTGCAACACTTCCACTTCGCGCATCATGCTGTGGCAGGAATGGAAAGTTA  >  1/1832100‑1832569

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑