Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,832,100	A→G	intergenic (+50200/+3284)	unknown → / ← unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,832,100	0	A	G	100.0%	210.9 / NA	71	intergenic (+50200/+3284)	unknown/unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (34/37);  total (34/37)

GATTACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGAGGAGGCGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTGCCATTGTTCCACGGAATAACGGTTTTGCAGCGTTTGGAACCAGTCGAATAACGCCGCTAAAAATGCCGCCAATTGCCCTGCCAGTTGCCCTTTCAAACCGAAACTGTTATCCAGCCCGAGGCTATCCTGCCAAATGCCGTTTTCTTCGCGCAAGGCATAACCCAATAACATTCGCTCCAAGCCGGCTTGCCATGAATTGTAGTTACTTTCCGCCTCATCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCACGAATTTGCGCCAAATCCTGCAATTCAATATTAAAGCGTTGGCGAATTGCCGGCACATCAAGCAATGCCAGCACGTCTTCGGCACTAAACGTGCTTTCTTTCAGCTGCAATAAGGTCAGAAAACCGGCGATAATCACATT  >  1/1831854‑1832345                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      gatcaCCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTGCCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGccc                                                                                                                                                                                                                                                    <  1:684274/246‑1 (MQ=255)     aCCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTGCCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCaacaa                                                                                                                                                                                                                                               <  1:705627/251‑4 (MQ=255)      ccTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTGCCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCaacaa                                                                                                                                                                                                                                               <  1:887714/250‑4 (MQ=255)      ccTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTGCCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATACAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCaacaa                                                                                                                                                                                                                                               >  1:580066/1‑247 (MQ=255)         ctTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTGAATGCTGTTGCCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACAtt                                                                                                                                                                                                                                           >  1:89547/1‑251 (MQ=255)         ctTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTGCCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACAtt                                                                                                                                                                                                                                           >  1:876212/1‑251 (MQ=255)         ctTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTGCCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACAtt                                                                                                                                                                                                                                           >  1:440998/1‑251 (MQ=255)         ctTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTGCCATGGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCaacaac                                                                                                                                                                                                                                              <  1:404094/248‑1 (MQ=255)              atatAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTGCCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCaacaac                                                                                                                                                                                                                                              <  1:451778/243‑1 (MQ=255)              atatAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTGCCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCt                                                                                                                                                                                                                                       >  1:368621/1‑250 (MQ=255)              atatAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTGCCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCt                                                                                                                                                                                                                                       >  1:496790/1‑250 (MQ=255)                    aaCAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTGCCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGcc                                                                                                                                                                                                                                <  1:593376/251‑1 (MQ=255)                    aaCAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTGCCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGcc                                                                                                                                                                                                                                <  1:566443/251‑1 (MQ=255)                          gTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTGCCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGcccgc                                                                                                                                                                                                                             >  1:625939/1‑245 (MQ=255)                             tCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTGCCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCa                                                                                                                                                                                                                       >  1:323364/1‑251 (MQ=255)                                   gTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTGCCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAAt                                                                                                                                                                                                                  <  1:115079/250‑1 (MQ=255)                                       ccgccgTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTGCCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAg                                                                                                                                                                                                             <  1:646285/251‑1 (MQ=255)                                          ccgtggcggagaagaaTTTATCGGTGAGCGCCAGTAAATGCTGTTGCCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTa                                                                                                                                                                                                          <  1:654118/241‑1 (MQ=255)                                                            tATCGGTGAGCGCCAGTAAATGCTGTTGCCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCAttt                                                                                                                                                                                        <  1:839993/251‑1 (MQ=255)                                                             aTCGGTGAGCGCCAGTAAATGCTGTTGCCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCaacaa                                                                                                                                                                                                                                               >  1:548496/1‑192 (MQ=255)                                                                 gTGAGCGCCAGTAAATGCTGTTGCCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGcc                                                                                                                                                                                                                                >  1:14466/1‑206 (MQ=255)                                                                        ccAGTAAATGCTGTTGCCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCg                                                                                                                                                                            >  1:190298/1‑251 (MQ=255)                                                                          aGTAAATGCTGTTGCCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGc                                                                                                                                                                                                  <  1:691953/227‑1 (MQ=255)                                                                                             gTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCGCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCAttttt                                                                                                                                                        >  1:439682/1‑250 (MQ=255)                                                                                             gTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCAttttt                                                                                                                                                        >  1:380612/1‑250 (MQ=255)                                                                                                                       gTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACGTTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAAt                                                                                                                              >  1:11493/1‑250 (MQ=255)                                                                                                                       gTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACt                                                                                                                                                                                                        <  1:246449/176‑1 (MQ=255)                                                                                                                       gTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAAt                                                                                                                              >  1:294515/1‑250 (MQ=255)                                                                                                                       gTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCC‑GCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATc                                                                                                                             >  1:132815/1‑250 (MQ=255)                                                                                                                        tttGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATc                                                                                                                             >  1:404536/1‑250 (MQ=255)                                                                                                                        tttGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATc                                                                                                                             <  1:814041/250‑1 (MQ=255)                                                                                                                                caatcaaaTAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTc                                                                                                                    <  1:776139/245‑1 (MQ=255)                                                                                                                                    caaaTAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAata                                                                                                                >  1:357684/3‑251 (MQ=255)                                                                                                                                     aaaTAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAatat                                                                                                               <  1:407042/250‑1 (MQ=255)                                                                                                                                          ccgctgcTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAg                                                                                                          <  1:440149/246‑1 (MQ=255)                                                                                                                                          aCGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAg                                                                                                          <  1:801952/251‑1 (MQ=255)                                                                                                                                          aCGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAg                                                                                                          <  1:443594/251‑1 (MQ=255)                                                                                                                                          aCGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAg                                                                                                          <  1:452350/251‑1 (MQ=255)                                                                                                                                          aCGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCAAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAg                                                                                                          <  1:319988/251‑1 (MQ=255)                                                                                                                                             ctgcTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTGGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAGCg                                                                                                        <  1:246877/248‑1 (MQ=255)                                                                                                                                               gcTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAGGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAGCGTTg                                                                                                     >  1:113912/1‑251 (MQ=255)                                                                                                                                               gcTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAacg                                                                                                                                                                       >  1:360994/1‑185 (MQ=255)                                                                                                                                               gcTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAGCGTTg                                                                                                     >  1:468304/1‑251 (MQ=255)                                                                                                                                                                     ccgCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAGCGTTGGCGAATTGCCGGCACATCAAGc                                                                               <  1:56062/249‑1 (MQ=255)                                                                                                                                                                              gCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAGCGTTGGCGAATTGCCGGCACATCAAGCAATGCCAg                                                                       >  1:860987/1‑250 (MQ=255)                                                                                                                                                                              gCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAGCGTTGGCGAATTGCCGGCACATCAAGCAATGCCAg                                                                       >  1:806617/1‑250 (MQ=255)                                                                                                                                                                                ccTTTTAAGCCGAACCTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAGCGTTGGCGAATTGCCGGCACATCAAGCAATGCCAGCAc                                                                    <  1:550280/251‑1 (MQ=255)                                                                                                                                                                                               cTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAGCg                                                                                                        <  1:128693/200‑1 (MQ=255)                                                                                                                                                                                               cTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAGCGTTGGCGAATTGCCGGCACATCAAGCAATGCCAGCACGTCTTCTGCACTaaa                                                     >  1:135265/1‑251 (MQ=255)                                                                                                                                                                                               cTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAGCGTTGGCGAATTGCCGGCACATCAAGCAATGCCAGCACGTCTTCTGCACTaaa                                                     >  1:218062/1‑251 (MQ=255)                                                                                                                                                                                                 gTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAGCGTTGGCGAATTGCCGGCACATCAAGCAATGCCAGCACGTCTTCTGCACTAAACg                                                   >  1:202035/1‑251 (MQ=255)                                                                                                                                                                                                    aTCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCCAATCCTGCAATTCAATATTAAAGCGTTGGCGAATTGCCGGCACATCAAGCa                                                                              >  1:437681/1‑221 (MQ=255)                                                                                                                                                                                                    aTCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAGCGTTGGCGAATTGCCGGCACATCAAGCAATGCCAGCACGTCTTCTGCACTAAACGTGc                                                <  1:595021/251‑1 (MQ=255)                                                                                                                                                                                                         gCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCTGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAGCGTTGGCGAATTGCCGGCACATCAAGCAATGCCAGCACGTCTTCTGCACTaa                                                      <  1:431087/240‑1 (MQ=255)                                                                                                                                                                                                         gCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAGCGTTGGCGAATTGCCGGCACATCAAGCa                                                                              <  1:570946/216‑1 (MQ=255)                                                                                                                                                                                                         gCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAGCGTTGGCGAATTGCCGGCACATCAAGCAATGCCAGCACGTCTTCTGCACTAAACGTGcttt                                             >  1:161995/1‑249 (MQ=255)                                                                                                                                                                                                           ccGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAGCGTTGGCGAATTGCCGGCACATCAAGCAATGCCAGCACGTCTTCTGCACTAAACGTGctttcttt                                         <  1:788461/251‑1 (MQ=255)                                                                                                                                                                                                                   gtcttGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAGCGTTGGCg                                                                                                  <  1:153750/182‑1 (MQ=255)                                                                                                                                                                                                                   gtcttGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAGCGTTGGCGAATTGCCGGCACATCAAGCAATGCCAGCACGTCTTCTGCACTAAACGTGCTTTCTTTCAGCTGCa                                 >  1:721358/5‑251 (MQ=255)                                                                                                                                                                                                                   gtcttGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAGCGTTGGCGAATTGCCGGCACATCAAGCAATGCCAGCACGTCTTCTGCACTAAACGTGCTTTCTTTCAGCTGCa                                 >  1:299997/5‑251 (MQ=255)                                                                                                                                                                                                                     cttGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAGCGTTGGCGAATTGCCGGCACATCAAGCAATGCCAGCACGTCTTCTGCACTAAACGTGCTTTCTTTCAGCTGCAAt                               <  1:146513/249‑1 (MQ=255)                                                                                                                                                                                                                      ttGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAGCGTTGGCGAATTGCCGGCACATCAAGCAATGCCAGCACGTCTTCTGCACTAAACGTGCTTTCTTTCAGCTGCAATa                              <  1:801318/250‑1 (MQ=255)                                                                                                                                                                                                                       tGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAGCGTTGGCGAATTGCCGGCACATCAAGCAATGCCAGCACGTCTTCTGCACTAAACGTGCTTTCTTTCAGCTGCAATaa                             <  1:571060/251‑1 (MQ=255)                                                                                                                                                                                                                         ccAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAGCGTTGGCGAATTGCCGGCACATCAAGCAATGCCAGCACGTCTTCTGCACTAAACGTGCTTTCTTTCAGCTGCAATAAgg                           <  1:574765/251‑1 (MQ=255)                                                                                                                                                                                                                               gCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAGCGTTGGCGAATTGCCGGCACATCAAGCAATGCCAGCACGTCTTCTGCACTAAACGTGCTTTCTTTCAGCTGCAATAAGGTCAGaa                     >  1:152891/1‑251 (MQ=255)                                                                                                                                                                                                                                ccGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAGCGTTGGCGAATTGCCGGCACATCAAGCAATGCCAGCACGTCTTCTGCACTAAACGTGCTTTCTTTCAGCTGCAATAAGGTCAGaaa                    <  1:865048/251‑1 (MQ=255)                                                                                                                                                                                                                                   ttttCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAGCGTTGGCGAATTGCCGGCACATCAAGCAATGCCAGCACGTCTTCTGCACTAAACGTGCTTTCTTTCAGCTGCAATAAGGTCAGAAAAc                  <  1:180665/250‑1 (MQ=255)                                                                                                                                                                                                                                         tcacgcAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAGCGTTGGCGAATTGCCGGCACATCAAGCAATGCCAGCACGTCTTCTGCACTAAACGTGCTTTCTTTCAGCTGCAATAAGGTCAGAAAACCGGCGAt           >  1:569349/4‑251 (MQ=255)                                                                                                                                                                                                                                           acgcAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAg                                                                                                          <  1:337541/153‑1 (MQ=255)                                                                                                                                                                                                                                                 ggCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAGCGTTGGCGAATTGCCGGCACATCAAGCAATGCCAGCACGTCTTCTGCAc                                                         >  1:640257/1‑197 (MQ=255)                                                                                                                                                                                                                                                  gCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAGCGTTGGCGAATTGCCGGCACATCAAGCAATGCCAGCACGTCTTCTGCACTAAACGTGCTTTCTTTCAGCTGCAATAAGGTCAGAAAACCGGCGATAATcacgtt  >  1:126225/1‑248 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      GATTACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGAGGAGGCGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTGCCATTGTTCCACGGAATAACGGTTTTGCAGCGTTTGGAACCAGTCGAATAACGCCGCTAAAAATGCCGCCAATTGCCCTGCCAGTTGCCCTTTCAAACCGAAACTGTTATCCAGCCCGAGGCTATCCTGCCAAATGCCGTTTTCTTCGCGCAAGGCATAACCCAATAACATTCGCTCCAAGCCGGCTTGCCATGAATTGTAGTTACTTTCCGCCTCATCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCACGAATTTGCGCCAAATCCTGCAATTCAATATTAAAGCGTTGGCGAATTGCCGGCACATCAAGCAATGCCAGCACGTCTTCGGCACTAAACGTGCTTTCTTTCAGCTGCAATAAGGTCAGAAAACCGGCGATAATCACATT  >  1/1831854‑1832345

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑