Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,831,995	C→T	intergenic (+50095/+3389)	unknown → / ← unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,831,995	0	C	T	100.0%	193.1 / NA	67	intergenic (+50095/+3389)	unknown/unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (32/35);  total (32/35)

TCGGATTTTCCTCCAACGCATTATTCATGACATCCGCCACCACATCCACGCCCAACGCGTCGCTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGTCGGATTACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGAGGAGGCGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTG‑CCATTGTTCCACGGAATAACGGTTTTGCAGCGTTTGGAACCAGTCGAATAACGCCGCTAAAAATGCCGCCAATTGCCCTGCCAGTTGCCCTTTCAAACCGAAACTGTTATCCAGCCCGAGGCTATCCTGCCAAATGCCGTTTTCTTCGCGCAAGGCATAACCCAATAACATTCGCTCCAAGCCGGCTTGCCATGAATTGTAGTTACTTTCCGCCTCATCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCACGAATTTGCGCCAAATCCTGCAATTCAATATTAAAG  >  1/1831754‑1832241                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                       tCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTG‑CCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAAt                                                                                                                                                                                                                                               <  1:10859/251‑1 (MQ=255) tCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTG‑CCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAAt                                                                                                                                                                                                                                               <  1:393527/251‑1 (MQ=255)               aaCGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTG‑CCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGccc                                                                                                                                                                                                                                 <  1:612989/251‑1 (MQ=255)                       gttCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTG‑CCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAg                                                                                                                                                                                                                           >  1:624419/2‑249 (MQ=255)                            tGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTG‑CCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGcc                                                                                                                                                                                                                      >  1:870207/1‑249 (MQ=255)                                     ccaccaCATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTG‑CCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCttttaagccg                                                                                                                                                                                                           >  1:518631/1‑244 (MQ=255)                                                   cccAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTG‑CCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAg                                                                                                                                                                                             >  1:896959/1‑251 (MQ=255)                                                                     tGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTG‑CCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCa                                                                                                                                                                           <  1:763171/251‑1 (MQ=255)                                                                      gCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTG‑CCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCaa                                                                                                                                                                          >  1:568036/1‑251 (MQ=255)                                                                       cACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTG‑CCATTGTTCCATGGAAT‑ACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAAt                                                                                                                                                                        >  1:464813/1‑251 (MQ=255)                                                                           tCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTG‑CCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCa                                                                                                                                                                           >  1:142744/1‑245 (MQ=255)                                                                           tCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTG‑CCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGc                                                                                                                                                                      >  1:539772/1‑250 (MQ=255)                                                                            ccGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTG‑CCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAgc                                                                                                                                                                                                                          <  1:530885/197‑2 (MQ=255)                                                                            ccGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTG‑CCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATcc                                                                                                                                                                                               <  1:352391/224‑1 (MQ=255)                                                                            ccGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTG‑CCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCg                                                                                                                                                                    >  1:603449/1‑251 (MQ=255)                                                                                             aattgctggATCACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGCGAGCGCCAGTAAATGCTGTTG‑CCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGc                                                                                                                                                   <  1:546203/244‑1 (MQ=255)                                                                                                 gctggATCACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTG‑CCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATag                                                                                                                                               >  1:74938/4‑250 (MQ=255)                                                                                                   tggATCACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTG‑CCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATagcc                                                                                                                                             <  1:85432/250‑4 (MQ=255)                                                                                                     gatcaCCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTG‑CCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGccc                                                                                                                                            <  1:684274/246‑1 (MQ=255)                                                                                                         aCCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTG‑CCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCaacaa                                                                                                                                       <  1:705627/251‑4 (MQ=255)                                                                                                          ccTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTGCCCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATagcc                                                                                                                                             >  1:762790/1‑242 (MQ=255)                                                                                                          ccTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTG‑CCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATcc                                                                                                                                                                                               <  1:48554/194‑1 (MQ=255)                                                                                                          ccTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTG‑CCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCaacaa                                                                                                                                       <  1:887714/250‑4 (MQ=255)                                                                                                          ccTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTG‑CCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATACAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCaacaa                                                                                                                                       >  1:580066/1‑247 (MQ=255)                                                                                                             ctTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTGAATGCTGTTG‑CCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACAtt                                                                                                                                   >  1:89547/1‑251 (MQ=255)                                                                                                             ctTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTG‑CCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCttttaagc                                                                                                                                                                                                             <  1:895172/177‑6 (MQ=255)                                                                                                             ctTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTG‑CCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCa                                                                                                                                                                           <  1:156334/211‑1 (MQ=255)                                                                                                             ctTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTG‑CCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCa                                                                                                                                                  <  1:130040/236‑1 (MQ=255)                                                                                                             ctTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTG‑CCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACAtt                                                                                                                                   >  1:440998/1‑251 (MQ=255)                                                                                                             ctTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTG‑CCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACAtt                                                                                                                                   >  1:876212/1‑251 (MQ=255)                                                                                                             ctTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTG‑CCATGGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCaacaac                                                                                                                                      <  1:404094/248‑1 (MQ=255)                                                                                                                  atatAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTG‑CCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGc                                                                                                                                                                                     >  1:145219/1‑196 (MQ=255)                                                                                                                  atatAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTG‑CCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCacgc                                                                                                                                                        <  1:499477/225‑1 (MQ=255)                                                                                                                  atatAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTG‑CCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCaacaac                                                                                                                                      <  1:451778/243‑1 (MQ=255)                                                                                                                  atatAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTG‑CCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCt                                                                                                                               >  1:496790/1‑250 (MQ=255)                                                                                                                  atatAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTG‑CCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCt                                                                                                                               >  1:368621/1‑250 (MQ=255)                                                                                                                       aaaCAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTG‑CCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCttttaagc                                                                                                                                                                                                             <  1:374114/167‑6 (MQ=255)                                                                                                                        aaCAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTG‑CCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGcc                                                                                                                        <  1:566443/251‑1 (MQ=255)                                                                                                                        aaCAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTG‑CCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGcc                                                                                                                        <  1:593376/251‑1 (MQ=255)                                                                                                                              gTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTG‑CCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAGCCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATagcc                                                                                                                                             <  1:664409/224‑4 (MQ=255)                                                                                                                              gTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTG‑CCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGcccgc                                                                                                                     >  1:625939/1‑245 (MQ=255)                                                                                                                                 tCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTG‑CCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCa                                                                                                               >  1:323364/1‑251 (MQ=255)                                                                                                                                       gTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTG‑CCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAAt                                                                                                          <  1:115079/250‑1 (MQ=255)                                                                                                                                           ccgccgTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTG‑CCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAg                                                                                                     <  1:646285/251‑1 (MQ=255)                                                                                                                                              ccgtggcggagaagaaTTTATCGGTGAGCGCCAGTAAATGCTGTTG‑CCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTa                                                                                                  <  1:654118/241‑1 (MQ=255)                                                                                                                                                               ttATCGGTGAGCGCCAGTAAATGCTGTTG‑CCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATcc                                                                                                                                                                                               >  1:383515/1‑141 (MQ=255)                                                                                                                                                                tATCGGTGAGCGCCAGTAAATGCTGTTG‑CCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCAttt                                                                                <  1:839993/251‑1 (MQ=255)                                                                                                                                                                 aTCGGTGAGCGCCAGTAAATGCTGTTG‑CCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCaacaa                                                                                                                                       >  1:548496/1‑192 (MQ=255)                                                                                                                                                                     gTGAGCGCCAGTAAATGCTGTTG‑CCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGcc                                                                                                                        >  1:14466/1‑206 (MQ=255)                                                                                                                                                                            ccAGTAAATGCTGTTG‑CCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCg                                                                    >  1:190298/1‑251 (MQ=255)                                                                                                                                                                              aGTAAATGCTGTTG‑CCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGc                                                                                          <  1:691953/227‑1 (MQ=255)                                                                                                                                                                                                  gTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCGCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCAttttt                                                >  1:439682/1‑250 (MQ=255)                                                                                                                                                                                                  gTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCAttttt                                                >  1:380612/1‑250 (MQ=255)                                                                                                                                                                                                                            gTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCAt                                                                                                                                                 >  1:672612/1‑127 (MQ=255)                                                                                                                                                                                                                            gTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACGTTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAAt                      >  1:11493/1‑250 (MQ=255)                                                                                                                                                                                                                            gTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACt                                                                                                <  1:246449/176‑1 (MQ=255)                                                                                                                                                                                                                            gTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAAt                      >  1:294515/1‑250 (MQ=255)                                                                                                                                                                                                                            gTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCC‑GCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATc                     >  1:132815/1‑250 (MQ=255)                                                                                                                                                                                                                             tttGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATc                     >  1:404536/1‑250 (MQ=255)                                                                                                                                                                                                                             tttGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATc                     <  1:814041/250‑1 (MQ=255)                                                                                                                                                                                                                                     caatcaaaTAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTc            <  1:776139/245‑1 (MQ=255)                                                                                                                                                                                                                                         caaaTAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAata        >  1:357684/3‑251 (MQ=255)                                                                                                                                                                                                                                          aaaTAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAatat       <  1:407042/250‑1 (MQ=255)                                                                                                                                                                                                                                               aCGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAg  <  1:801952/251‑1 (MQ=255)                                                                                                                                                                                                                                               aCGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAg  <  1:443594/251‑1 (MQ=255)                                                                                                                                                                                                                                               aCGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAg  <  1:452350/251‑1 (MQ=255)                                                                                                                                                                                                                                               aCGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCAAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAg  <  1:319988/251‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                       TCGGATTTTCCTCCAACGCATTATTCATGACATCCGCCACCACATCCACGCCCAACGCGTCGCTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGTCGGATTACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGAGGAGGCGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTG‑CCATTGTTCCACGGAATAACGGTTTTGCAGCGTTTGGAACCAGTCGAATAACGCCGCTAAAAATGCCGCCAATTGCCCTGCCAGTTGCCCTTTCAAACCGAAACTGTTATCCAGCCCGAGGCTATCCTGCCAAATGCCGTTTTCTTCGCGCAAGGCATAACCCAATAACATTCGCTCCAAGCCGGCTTGCCATGAATTGTAGTTACTTTCCGCCTCATCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCACGAATTTGCGCCAAATCCTGCAATTCAATATTAAAG  >  1/1831754‑1832241

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑