Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,831,857	T→C	intergenic (+49957/+3527)	unknown → / ← unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,831,857	0	T	C	100.0%	187.1 / NA	63	intergenic (+49957/+3527)	unknown/unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/42);  total (21/42)

ATTAAATCGAAACTGTTCGGCGCTTGTTGGCGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACCACTTTGAACGGAATGGAGCGCATCGGCAACAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGATAATGGTTCGGATTTTCCTCCAACGCATTATTCATGACATCCGCCACCACATCCACGCCCAACGCGTCGCTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGTCGGATTACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGAGGAGGCGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTGCCATTGTTCCACGGAATAACGGTTTTGCAGCGTTTGGAACCAGTCGAATAACGCCGCTAAAAATGCCGCCAATTGCCCTGCCAGTTGCCCTTTCAAACCGAAACTGTTATCCAGCCCGAGGCTATCCTGCCAAATGCCGTTTTCTTCGCGCAAGGCATAACC  >  1/1831612‑1832102                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      aTTAAATCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACctc                                                                                                                                                                                                                                                  <  1:14748/251‑1 (MQ=255)      aTCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTaa                                                                                                                                                                                                                                             <  1:617525/251‑1 (MQ=255)      aTCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTaa                                                                                                                                                                                                                                             <  1:786969/251‑1 (MQ=255)          aaaCTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAatat                                                                                                                                                                                                                                          <  1:821291/250‑1 (MQ=255)          aaaCTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAAtata                                                                                                                                                                                                                                         >  1:534565/1‑251 (MQ=255)            aCTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTaa                                                                                                                                                                                                                                             >  1:727643/1‑245 (MQ=255)              tGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAAc                                                                                                                                                                                                                                     <  1:811508/251‑1 (MQ=255)              tGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAAc                                                                                                                                                                                                                                     <  1:322303/251‑1 (MQ=255)              tGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAAc                                                                                                                                                                                                                                     <  1:777041/251‑1 (MQ=255)                    gcgcTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGt                                                                                                                                                                                                                                <  1:845299/250‑1 (MQ=255)                     cgcTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGATCGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAATGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGtt                                                                                                                                                                                                                               <  1:381713/250‑1 (MQ=255)                                   ggTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTaa                                                                                                                                                                                                                                             >  1:670424/1‑222 (MQ=255)                                   ggTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAAc                                                                                                                                                                                                                                     <  1:397597/230‑1 (MQ=255)                                    gTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAatat                                                                                                                                                                                                                                          <  1:209463/224‑1 (MQ=255)                                     tATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAAc                                                                                                                                                                                                                                     <  1:271996/228‑1 (MQ=255)                                              tcgtTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGTTTCATCGGTTTccgccgtggcggagaa                                                                                                                                                                                                     >  1:578325/1‑241 (MQ=255)                                               cgtTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGTTTCATCGGTTTccgccgtggcggagaag                                                                                                                                                                                                    >  1:205563/1‑240 (MQ=255)                                                gtTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGt                                                                                                                                                                                                                                <  1:427831/222‑1 (MQ=255)                                                       ccGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATc                                                                                                                                                                                            <  1:66918/251‑1 (MQ=255)                                                       ccGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATc                                                                                                                                                                                            <  1:557273/251‑1 (MQ=255)                                                                      actctGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTaa                                                                                                                                                                             >  1:801674/5‑251 (MQ=255)                                                                          tGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGc                                                                                                                                                                         <  1:871585/251‑1 (MQ=255)                                                                            aaCGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTg                                                                                                                                                                       <  1:479328/251‑1 (MQ=255)                                                                               ggAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAAt                                                                                                                                                                           >  1:300040/1‑244 (MQ=255)                                                                                gAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAAc                                                                                                                                                                                                                                     >  1:102567/1‑185 (MQ=255)                                                                                gAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTg                                                                                                                                                                       <  1:752146/247‑1 (MQ=255)                                                                                      aGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTGCCAtt                                                                                                                                                               <  1:761445/249‑1 (MQ=255)                                                                                        cgcATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTGCCATTGTTc                                                                                                                                                           <  1:743213/251‑1 (MQ=255)                                                                                        cgcATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTGCCATTGTTc                                                                                                                                                           <  1:791672/251‑1 (MQ=255)                                                                                                     aTGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTGCCATTGTTCCATGGAATAACgg                                                                                                                                              <  1:592100/251‑1 (MQ=255)                                                                                                                       tGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTGCCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAAc                                                                                                                            <  1:697711/251‑1 (MQ=255)                                                                                                                       tGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTGCCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAAc                                                                                                                            <  1:689372/251‑1 (MQ=255)                                                                                                                                  aagcggtagtgattCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTGCCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATa                                                                                                                  <  1:512537/238‑1 (MQ=255)                                                                                                                                    gcggtagtgattCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCGCCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTaa                                                                                                                                                                                                                                             <  1:263309/115‑1 (MQ=255)                                                                                                                                               tCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTGCCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAAt                                                                                                    <  1:10859/251‑1 (MQ=255)                                                                                                                                               tCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTGCCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAAt                                                                                                    <  1:393527/251‑1 (MQ=255)                                                                                                                                                    ttttCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAgcgc                                                                                                                                                                                   <  1:584096/167‑1 (MQ=255)                                                                                                                                                             aaCGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTGCCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGccc                                                                                      <  1:612989/251‑1 (MQ=255)                                                                                                                                                                   ttgttCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTGCCATTGTTCCATGGAATAACggc                                                                                                                                             <  1:223376/187‑2 (MQ=255)                                                                                                                                                                     gttCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGTTTCATc                                                                                                                                                                                                                          <  1:67912/110‑1 (MQ=255)                                                                                                                                                                     gttCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTGCCATTGTTc                                                                                                                                                           <  1:80447/173‑1 (MQ=255)                                                                                                                                                                     gttCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTGCCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGa                                                                                                                              >  1:596344/2‑203 (MQ=255)                                                                                                                                                                     gttCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTGCCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGa                                                                                                                              >  1:828314/2‑203 (MQ=255)                                                                                                                                                                     gttCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTGCCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAg                                                                                >  1:624419/2‑249 (MQ=255)                                                                                                                                                                          tGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTGCCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGcc                                                                           >  1:870207/1‑249 (MQ=255)                                                                                                                                                                                   ccaccaCATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTGCCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCttttaagccg                                                                >  1:518631/1‑244 (MQ=255)                                                                                                                                                                                                 cccAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTGCCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATaa                                                                                                                 <  1:693654/188‑1 (MQ=255)                                                                                                                                                                                                 cccAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTGCCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAg                                                  >  1:896959/1‑251 (MQ=255)                                                                                                                                                                                                                   tGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTGCCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCa                                <  1:763171/251‑1 (MQ=255)                                                                                                                                                                                                                    gCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTGCCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCaa                               >  1:568036/1‑251 (MQ=255)                                                                                                                                                                                                                     cACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTGCCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAAc                                                                                                                            >  1:144235/1‑157 (MQ=255)                                                                                                                                                                                                                     cACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTGCCATTGTTCCATGGAAT‑ACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAAt                             >  1:464813/1‑251 (MQ=255)                                                                                                                                                                                                                         tCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTGCCATTGTTCCATGGAATAACgg                                                                                                                                              <  1:734155/135‑1 (MQ=255)                                                                                                                                                                                                                         tCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTGCCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAAc                                                                                                                            <  1:241663/153‑1 (MQ=255)                                                                                                                                                                                                                         tCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTGCCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCa                                >  1:142744/1‑245 (MQ=255)                                                                                                                                                                                                                         tCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTGCCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGc                           >  1:539772/1‑250 (MQ=255)                                                                                                                                                                                                                          ccGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTGCCATTGTTCCATGGAATAACGGCTTTGCAg                                                                                                                                      <  1:487683/142‑1 (MQ=255)                                                                                                                                                                                                                          ccGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTGCCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAgc                                                                               <  1:530885/197‑2 (MQ=255)                                                                                                                                                                                                                          ccGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTGCCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATcc                                                    <  1:352391/224‑1 (MQ=255)                                                                                                                                                                                                                          ccGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTGCCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCg                         >  1:603449/1‑251 (MQ=255)                                                                                                                                                                                                                                           aattgctggATCACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGCGAGCGCCAGTAAATGCTGTTGCCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGc        <  1:546203/244‑1 (MQ=255)                                                                                                                                                                                                                                               gctggATCACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTGCCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATag    >  1:74938/4‑250 (MQ=255)                                                                                                                                                                                                                                                 tggATCACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTGCCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATagcc  <  1:85432/250‑4 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      ATTAAATCGAAACTGTTCGGCGCTTGTTGGCGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACCACTTTGAACGGAATGGAGCGCATCGGCAACAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGATAATGGTTCGGATTTTCCTCCAACGCATTATTCATGACATCCGCCACCACATCCACGCCCAACGCGTCGCTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGTCGGATTACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGAGGAGGCGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTGCCATTGTTCCACGGAATAACGGTTTTGCAGCGTTTGGAACCAGTCGAATAACGCCGCTAAAAATGCCGCCAATTGCCCTGCCAGTTGCCCTTTCAAACCGAAACTGTTATCCAGCCCGAGGCTATCCTGCCAAATGCCGTTTTCTTCGCGCAAGGCATAACC  >  1/1831612‑1832102

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑