Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,831,776	A→G	intergenic (+49876/+3608)	unknown → / ← unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,831,776	0	A	G	100.0%	187.5 / NA	64	intergenic (+49876/+3608)	unknown/unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/46);  total (18/46)

CCGACAGCAAGGCTTCAAGGAACAGATAACGATCGTCATCGCGACGGGCGCGATCGCCTTTTAAGCGGTGATATTGCATTAAATCGAAACTGTTCGGCGCTTGTTGGCGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACCACTTTGAACGGAATGGAGCGCATCGGCAACAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGATAATGGTTCGGATTTTCCTCCAACGCATTATTCATGACATCCGCCACCACATCCACGCCCAACGCGTCGCTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGTCGGATTACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGAGGAGGCGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTGCCATTGTTCCACGGAATAACGGTTTTGCAGCGTTTGGAACCAGTCGAATAACGCCGCTAAAAATGCCGCCAATTGCCC  >  1/1831535‑1832018                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                   ccGACAGCAAGGCTTCAAGGAACAGATAACGATCGTCATCACGACGGGCGCGATCGCCTTTTAAACGATGATATTGCATTAAATCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGAc                                                                                                                                                                                                                                            <  1:750877/250‑1 (MQ=255)   gACAGCAAGGCTTCAAGGAACAGATAACGATCGTCATCACGACGGGCGCGATCGCCTTTTAAACGATGATATTGCATTAAATCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCATCCAACGCATTGTTCATGACAt                                                                                                                                                                                                                                          >  1:15892/1‑250 (MQ=255)           ggCTTCAAGGAACAGATAACGATCGTCATCACGACGGGCGCGATCGCCTTTTAAACGATGATATTGCATTAAATCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATctg                                                                                                                                                                                                                                       <  1:461484/245‑3 (MQ=255)             cTTCAAGGAACAGATAACGATCGTCATCACGACGGGCGCGATCGCCTTTTAAACGATGATATTGCATTAAATCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACcaca                                                                                                                                                                                                                               <  1:267484/251‑1 (MQ=255)                cAAGGAACAGATAACGATCGTCATCACGACGGGCGCGATCGCCTTTTAAACGATGATATTGCATTAAATCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATcc                                                                                                                                                                                                                            <  1:206365/251‑1 (MQ=255)                      aCAGATAACGATCGTCATCACGACGGGCGCGATCGCCTTTTAAACGATGATATTGCATTAAATCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGcc                                                                                                                                                                                                                       >  1:248450/1‑250 (MQ=255)                         gATAACGATCGTCATCACGACGGGCGCGATCGCCTTTTAAACGATGATATTGCATTAAATCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGAc                                                                                                                                                                                                                                            >  1:93721/1‑226 (MQ=255)                                aTCGTCATCACGACGGGCGCGATCGCCTTTTAAACGATGATATTGCATTAAATCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATcc                                                                                                                                                                                                                            >  1:74318/1‑235 (MQ=255)                                       tcacgACGGGCGCGATCGCCTTTTAAACGATGATATTGCATTAAATCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATg                                                                                                                                                                                                     <  1:794054/248‑1 (MQ=255)                                          cgACGGGCGCGATCGCCTTTTAAACGATGATATTGCATTAAATCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCa                                                                                                                                                                                                   >  1:259187/1‑250 (MQ=255)                                               ggCGCGATCGCCTTTTAAACGATGATATTGCATTAAATCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGccc                                                                                                                                                                                                                      >  1:267651/1‑226 (MQ=255)                                                     aTCGCCTTTTAAACGATGATATTGCATTAAATCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAAc                                                                                                                                                                                        <  1:579490/250‑1 (MQ=255)                                                     aTCGCCTTTTAAACGATGATATTGCATTAAATCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACt                                                                                                                                                                                       <  1:253501/251‑1 (MQ=255)                                                      tCGCCTTTTAAACGATGATATTGCATTAAATCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTg                                                                                                                                                                                      <  1:586312/251‑1 (MQ=255)                                                                       atatTGCATTAAATCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACa                                                                                                                                                                                                                                           <  1:521065/181‑1 (MQ=255)                                                                       atatTGCATTAAATCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGc                                                                                                                                                                                                                        <  1:26919/200‑1 (MQ=255)                                                                        tatTGCATTAAATCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCGCCTCCGCTAACTGTTCGGCAAATTgctggat                                                                                                                                                                    <  1:581962/251‑7 (MQ=255)                                                                        tatTGCATTAAATCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTgctggat                                                                                                                                                                    <  1:375007/251‑7 (MQ=255)                                                                            gCATTAAATCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTgctggatcac                                                                                                                                                                 >  1:409057/1‑241 (MQ=255)                                                                              aTTAAATCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACctc                                                                                                                                                              <  1:14748/251‑1 (MQ=255)                                                                                   aTCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTaa                                                                                                                                                         <  1:617525/251‑1 (MQ=255)                                                                                   aTCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTaa                                                                                                                                                         <  1:786969/251‑1 (MQ=255)                                                                                       aaaCTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAatat                                                                                                                                                      <  1:821291/250‑1 (MQ=255)                                                                                       aaaCTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAAtata                                                                                                                                                     >  1:534565/1‑251 (MQ=255)                                                                                         aCTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTaa                                                                                                                                                         >  1:727643/1‑245 (MQ=255)                                                                                           tGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAAc                                                                                                                                                 <  1:811508/251‑1 (MQ=255)                                                                                           tGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAAc                                                                                                                                                 <  1:322303/251‑1 (MQ=255)                                                                                           tGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAAc                                                                                                                                                 <  1:777041/251‑1 (MQ=255)                                                                                            gTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAAc                                                                                                                                                                                        >  1:304761/1‑211 (MQ=255)                                                                                                 gcgcTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGt                                                                                                                                            <  1:845299/250‑1 (MQ=255)                                                                                                  cgcTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGATCGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAATGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGtt                                                                                                                                           <  1:381713/250‑1 (MQ=255)                                                                                                                ggTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTaa                                                                                                                                                         >  1:670424/1‑222 (MQ=255)                                                                                                                ggTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAAc                                                                                                                                                 <  1:397597/230‑1 (MQ=255)                                                                                                                 gTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTc                                                                                                                                                                                               <  1:21720/183‑1 (MQ=255)                                                                                                                 gTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAatat                                                                                                                                                      <  1:209463/224‑1 (MQ=255)                                                                                                                  tATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAAc                                                                                                                                                 <  1:271996/228‑1 (MQ=255)                                                                                                                           tcgtTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGTTTCATCGGTTTccgccgtggcggagaa                                                                                                                 >  1:578325/1‑241 (MQ=255)                                                                                                                            cgtTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGTTTCATCGGTTTccgccgtggcggagaag                                                                                                                >  1:205563/1‑240 (MQ=255)                                                                                                                            cgtTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCAGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCa                                                                                                                                                                                                                           >  1:565006/1‑144 (MQ=255)                                                                                                                             gtTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGt                                                                                                                                            <  1:427831/222‑1 (MQ=255)                                                                                                                                    ccGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATc                                                                                                        <  1:66918/251‑1 (MQ=255)                                                                                                                                    ccGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATc                                                                                                        <  1:557273/251‑1 (MQ=255)                                                                                                                                              aaaCTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTgctggat                                                                                                                                                                    <  1:379379/181‑7 (MQ=255)                                                                                                                                                   actctGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTaa                                                                                         >  1:801674/5‑251 (MQ=255)                                                                                                                                                       tGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGc                                                                                     <  1:871585/251‑1 (MQ=255)                                                                                                                                                         aaCGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTg                                                                                   <  1:479328/251‑1 (MQ=255)                                                                                                                                                            ggAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAAt                                                                                       >  1:300040/1‑244 (MQ=255)                                                                                                                                                             gAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGAc                                                                                                                                                                                                                                            <  1:753708/94‑1 (MQ=255)                                                                                                                                                             gAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAAc                                                                                                                                                                                        >  1:414319/1‑146 (MQ=255)                                                                                                                                                             gAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAAc                                                                                                                                                 >  1:102567/1‑185 (MQ=255)                                                                                                                                                             gAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTg                                                                                   <  1:752146/247‑1 (MQ=255)                                                                                                                                                                   aGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTGCCAtt                                                                           <  1:761445/249‑1 (MQ=255)                                                                                                                                                                     cgcATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTGCCATTGTTc                                                                       <  1:743213/251‑1 (MQ=255)                                                                                                                                                                     cgcATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTGCCATTGTTc                                                                       <  1:791672/251‑1 (MQ=255)                                                                                                                                                                            gcagcaaTGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCAcc                                                                                                                                                                                                 <  1:707211/118‑1 (MQ=255)                                                                                                                                                                                  aTGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTGCCATTGTTCCATGGAATAACgg                                                          <  1:592100/251‑1 (MQ=255)                                                                                                                                                                                                    tGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTGCCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAAc                                        <  1:697711/251‑1 (MQ=255)                                                                                                                                                                                                    tGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTGCCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAAc                                        <  1:689372/251‑1 (MQ=255)                                                                                                                                                                                                               aagcggtagtgattCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTGCCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATa                              <  1:512537/238‑1 (MQ=255)                                                                                                                                                                                                                 gcggtagtgattCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCGCCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTaa                                                                                                                                                         <  1:263309/115‑1 (MQ=255)                                                                                                                                                                                                                            tCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTGCCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAAt                <  1:393527/251‑1 (MQ=255)                                                                                                                                                                                                                            tCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTGCCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAAt                <  1:10859/251‑1 (MQ=255)                                                                                                                                                                                                                                 ttttCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAgcgc                                                                                               <  1:584096/167‑1 (MQ=255)                                                                                                                                                                                                                                          aaCGCATTGTTCATGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTGCCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGccc  <  1:612989/251‑1 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                   CCGACAGCAAGGCTTCAAGGAACAGATAACGATCGTCATCGCGACGGGCGCGATCGCCTTTTAAGCGGTGATATTGCATTAAATCGAAACTGTTCGGCGCTTGTTGGCGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACCACTTTGAACGGAATGGAGCGCATCGGCAACAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGATAATGGTTCGGATTTTCCTCCAACGCATTATTCATGACATCCGCCACCACATCCACGCCCAACGCGTCGCTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGTCGGATTACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGAGGAGGCGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTGCCATTGTTCCACGGAATAACGGTTTTGCAGCGTTTGGAACCAGTCGAATAACGCCGCTAAAAATGCCGCCAATTGCCC  >  1/1831535‑1832018

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑