Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,831,575	G→A	intergenic (+49675/+3809)	unknown → / ← unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,831,575	0	G	A	100.0%	178.0 / NA	63	intergenic (+49675/+3809)	unknown/unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (39/24);  total (39/24)

TTTTTCAAAGTTTTTACGACTGAACACGGTCATGCCATGTTGTTGAATCAGTCGTTCGCGCCACTTGAGATTTCCCGATGGGGTATTTTCCGCCGGCAGATTTTCCACGATGTAATCCAGCAACTGACTCACCAGCACCGACGGCTCCCGCTCCTGATTATCGGCAATAGAACGACCGATGTAGCTGATGTACAAATAATCCTGCGCCGACAGCAAGGCTTCAAGGAACAGATAACGATCGTCATCGCGACGGGCGCGATCGCCTTTTAAGCGGTGATATTGCATTAAATCGAAACTGTTCGGCGCTTGTTGGCGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACCACTTTGAACGGAATGGAGCGCATCGGCAACAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGATAATGGTTCGGATTTTCCTCCAACGCATTATTCATGACATCCGCCACCACATCCACGCC  >  1/1831329‑1831805                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                       tttttCAAAGTTTTTGCGACTGAACGCCGTCATGCCATGTTGTTGAATCAGTCGTTCGCGCCACTTGAGATTTCCCGCTGGGGTATTTTCTGCCGGCAGATTTTCCACGATGTAATCCAACAACTGACTCACCAGCACCGACGGCTCCCGTTCCTGATTATCGGCGATGGAACGTCCGATGTAGCTGATGTACAAATAATCCTGCGCCGACAGCAAGGCTTCAAGGAACAGATAACGATCGTCATCACGAc                                                                                                                                                                                                                                    <  1:48330/251‑1 (MQ=255)    ttCAAAGTTTTTGCGACTGAACGCCGTCATGCCATGTTGTTGAATCAGTCGTTCGCGCCACTTGAGATTTCCCGCTGGGGTATTTTCTGCCGGCAGATTTTCCACGATGTAATCCAACAACTGACTCACCAGCACCGACGGCTCCCGTTCCTGATTATCGGCGATGGAACGTCCGATGTAGCTGATGTACAAATAATCCTGCGCCGACAGCAAGGCTTCAAGGAACAGATAACGATCGTCATCACGACggg                                                                                                                                                                                                                                 <  1:448391/251‑1 (MQ=255)           tttttGCGACTGAACGCCGTCATGCCATGTTGTTGAATCAGTCGTTCGCGCCACTTGAGATTTCCCGCTGGGGTATTTTCTGCCGGCAGATTTTCCACGATGTAATCCAACAACTGACTCACCAGCACCGACGGCTCCCGTTCCTGATTATCGGCGATGGAACGTCCGATGTAGCTGATGTACAAATAATCCTGCGCCGACAGCAAGGCTTCAAGGAACAGATAACGATCGTCATCACGACGGGCGCGATc                                                                                                                                                                                                                          <  1:470865/251‑1 (MQ=255)            ttttGCGACTGAACGCCGTCATGCCATGTTGTTGAATCAGTCGTTCGCGCCACTTGAGATTTCCCGCTGGGGTATTTTCTGCCGGCAGATTTTCCACGATGTAATCCAACAACTGACTCACCAGCACCGACGGCTCCCGTTCCTGATTATCGGCGATGGAACGTCCGATGTAGCTGATGTACAAATAATCCTGCGCCGACAGCAAGGCTTCAAGGAACAGATAACGATCGTCATCACGACGGGCGCGATCg                                                                                                                                                                                                                         >  1:613964/1‑251 (MQ=255)                     tgaacgccgTCATGCCATGTTGTTGAATCAGTCGTTCGCGCCACTTGAGATTTCCCGCTGGGGTATTTTCTGCCGGCAGATTTTCCACGATGTAATCCAACAACTGACTCACCAGCACCGACGGCTCCCGTTCCTGATTATCGGCGATGGAACGTCCGATGTAGCTGATGTACAAATAATCCTGCGCCGACAGCAAGGCTTCAAGGAACAGATAACGATCGTCATCACGACGGGCGCGATCGCCTTTTaaa                                                                                                                                                                                                                <  1:328640/243‑2 (MQ=255)                             gTCATGCCATGTTGTTGAATCAGTCGTTCGCGCCACTTGAGATTTCCCGCTGGGGTATTTTCTGCCGGCAGATTTTCCACGATGTAATCCAACAACTGACTCACCAGCACCGACGGCTCCCGTTCCTGATTATCGGCGATGGAACGTCCGATGTAGCTGATGTACAAATAATCCTGCGCCGACAGCAAGGCTTCAAGGAACAGATAACGATCGTCATCACGACGGGCGCGATCGCCTTTTAAACGATGata                                                                                                                                                                                                        >  1:727960/1‑251 (MQ=255)                              tCATGCCATGTTGTTGAATCAGTCGTTCGCGCCACTTGAGATTTCCCGCTGGGGTATTTTCTGCCGGCAGATTTTCCACGATGTAATCCAACAACTGACTCACCAGCACCGACGGCTCCCGTTCCTGATTATCGGCGATGGAACGTCCGATGTAGCTGATGTACAAATAATCCTGCGCCGACAGCAAGGCTTCAAGGAACAGATAACGATCGTCATCACGACGGGCGCGATCGCCTTTTAAACGATGatat                                                                                                                                                                                                       <  1:810107/251‑1 (MQ=255)                                aTGCCATGTTGTTGAATCAGTCGTTCGCGCCACTTGAGATTTCCCGCTGGGGTATTTTCTGCCGGCAGATTTTCCACGATGTAATCCAACAACTGACTCACCAGCACCGACGGCTCCCGTTCCTGATTATCGGCGATGGAACGTCCGATGTAGCTGATGTACAAATAATCCTGCGCCGACAGCAAGGCTTCAAGGAACAGATAACGATCGTCATCACGACGGGCGCGATCGCCTTTTAAACGATGATATTg                                                                                                                                                                                                     >  1:512700/1‑251 (MQ=255)                                   ccATGTTGTTGAATCAGTCGTTCGCGCCACTTGAGATTTCCCGCTGGGGTATTTTCTGCCGGCAGATTTTCCACGATGTAATCCAACAACTGACTCACCAGCACCGACGGCTCCCGTTCCTGATTATCGGCGATGGAACGTCCGATGTAGCTGATGTACAAATAATCCTGCGCCGACAGCAAGGCTTCAAGGAACAGATAACGATCGTCATCACGACGGGCGCGATCGCCTTTTAAACGATGATATTGCAt                                                                                                                                                                                                  <  1:532992/251‑1 (MQ=255)                                     aTGTTGTTGAATCAGTCGTTCGCGCCACTTGAGATTTCCCGCTGGGGTATTTTCTGCCGGCAGATTTTCCACGATGTAATCCAACAACTGACTCACCAGCACCGACGGCTCCCGTTCCTGATTATCGGCGATGGAACGTCCGATGTAGCTGATGTACAAATAATCCTGCGCCGACAGCAAGGCTTCAAGGAACAGATAACGATCGTCATCACGACGGgcg                                                                                                                                                                                                                               >  1:10409/1‑220 (MQ=255)                                       gttgttGAATCAGTCGTTCGCGCCACTTGAGATTTCCCGCTGGGGTATTTTCTGCCGGCAGATTTTCCACGATGTAATCCAACAACTGACTCACCAGCACCGACGGCTCCCGTTCCTGATTATCGGCGATGGAACGTCCGATGTAGCTGATGTACAAATAATCCTGCGCCGACAGCAAGGCTTCAAGGAACAGATAACGATCGTCATCACGACGGGCGCGATCGCCTTTTAAACGATGATATTGCATTaaa                                                                                                                                                                                              >  1:666731/1‑251 (MQ=255)                                        ttgttgAATCAGTCGTTCGCGCCACTTGAGATTTCCCGCTGGGGTATTTTCTGCCGGCAGATTTTCCACGATGTAATCCAACAACTGACTCACCAGCACCGACGGCTCCCGTTCCTGATTATCGGCGATGGAACGTCCGATGTAGCTGATGTACAAATAATCCTGCGCCGACAGCAAGGCTTCAAGGAACAGATAACGATCGTCATCACGACGGGCGCGATCGCCTTTTAAACGATGATATTGCATTAAAt                                                                                                                                                                                             <  1:685163/251‑1 (MQ=255)                                             gAATCAGTCGTTCGCGCCACTTGAGATTTCCCGCTGGGGTATTTTCTGCCGGCAGATTTTCCACGATGTAATCCAACAACTGACTCACCAGCACCGACGGCTCCCGTTCCTGATTATCGGCGATGGAACGTCCGATGTAGCTGATGTACAAATAATCCTGCGCCGACAGCAAGGCTTCAAGGAACAGATAACGATCGTCATCACGACGGGCGCGATCGCCTTTTAAACGATGATATTGCATTAAATCGaaa                                                                                                                                                                                        >  1:696121/1‑251 (MQ=255)                                                   gTCGTTCGCGCCACTTGAGATTTCCCGCTGGGGTATTTTCTGCCGGCAGATTTTCCACGATGTAATCCAACAACTGACTCACCAGCACCGACGGCTCCCGTTCCTGATTATCGGCGATGGAACGTCCGATGTAGCTGATGTACAAATAATCCTGCGCCGACAGCAAGGCTTCAAGGAACAGATAACGATCGTCATCACGACGGGCGCGATCGCCTTTTAAACGATGatat                                                                                                                                                                                                       >  1:73391/1‑230 (MQ=255)                                                        tCGCGCCACTTGAGATTTCCCGCTGGGGTATTTTCTGCCGGCAGATTTTCCACGATGTAATCCAACAACTGACTCACCAGCACCGACGGCTCCCGTTCCTGATTATCGGCGATGGAACGTCCGATGTAGCTGATGTACAAATAATCCTGCGCCGACAGCAAGGCTTCAAGGAACAGATAACGATCGTCATCACGACGGGCGCGATCGCCTTTTAAACGATGATATTGCATTAAATCGAAACTGTTCGgcgc                                                                                                                                                                             <  1:272560/251‑1 (MQ=255)                                                             ccACTTGAGATTTCCCGCTGGGGTATTTTCTGCCGGCAGATTTTCCACGATGTAATCCAACAACTGACTCACCAGCACCGACGGCTCCCGTTCCTGATTATCGGCGATGGAACGTCCGATGTAGCTGATGTACAAATAATCCTGCGCCGACAGCAAGGCTTCAAGGAACAGATAACGATCGTCATCACGACGGGCGCGATCGCCTTTTAAACGATGATATTGCATTAAATCGAAACTGTTCGGCGCttgtt                                                                                                                                                                        >  1:100251/1‑251 (MQ=255)                                                               aCTTGAGATTTCCCGCTGGGGTATTTTCTGCCGGCAGATTTTCCACGATGTAATCCAACAACTGACTCACCAGCACCGACGGCTCCCGTTCCTGATTATCGGCGATGGAACGTCCGATGTAGCTGATGTACAAATAATCCTGCGCCGACAGCAAGGCTTCAAGGAACAGATAACGATCGTCATCACGACGGGCGCGATCGCCTTTTAAACGATGATATTGCATTAAATCGAAACTGTTCGGCGCttgttga                                                                                                                                                                      >  1:242367/1‑250 (MQ=255)                                                                        ttCCCGCTGGGGTATTTTCTGCCGGCAGATTTTCCACGATGTAATCCAACAACTGACTCACCAGCACCGACGGCTCCCGTTCCTGATTATCGGCGATGGAACGTCCGATGTAGCTGATGTACAAATAATCCTGCGCCGACAGCAAGGCTTCAAGGAACAGATAACGATCGTCATCACGACGGGCGCGATCGCCTTTTAAACGATGATATTGCATTAAATCGAAACTGTTCGGCGCTTGTTGACGCGGGTAt                                                                                                                                                             <  1:310672/251‑1 (MQ=255)                                                                                ggggTATTTTCTGCCGGCAGATTTTCCACGATGTAATCCAACAACTGACTCACCAGCACCGACGGCTCCCGTTCCTGATTATCGGCGATGGAACGTCCGATGTAGCTGATGTACAAATAATCCTGCGCCGACAGCAAGGCTTCAAGGAACAGATAACGATCGTCATCACGACGGGCGCGATCGCCTTTTAAACGATGATATTGCATTAAATCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCcgt                                                                                                                                                      >  1:534461/1‑250 (MQ=255)                                                                                ggggTATTTTCTGCCGGCAGATTTTCCACGATGTAATCCAACAACTGACTCACCAGCACCGACGGCTCCCGTTCCTGATTATCGGCGATGGAACGTCCGATGTAGCTGATGTACAAATAATCCTGCGCCGACAGCAAGGCTTCAAGGAACAGATAACGATCGTCATCACGACGGGCGCGATCGCCTTTTAAACGATGATATTGCATTAAATCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCcgt                                                                                                                                                      >  1:256208/1‑250 (MQ=255)                                                                                 gggTATTTTCTGCCGGCAGATTTTCCACGATGTAATCCAACAACTGACTCACCAGCACCGACGGCTCCCGTTCCTGATTATCGGCGATGGAACGTCCGATGTAGCTGATGTACAAATAATCCTGCGCCGACAGCAAGGCTTCAAGGAACAGATAACGATCGTCATCACGACGGGCGCGATCGCCTTTTAAACGATGATATTGCATTAAATCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCcgtcg                                                                                                                                                    >  1:158779/1‑251 (MQ=255)                                                                                        ttCCGCCGGCAGATTTTCCACGATGTAATCCAACAACTGACTCACCAGCACCGACGGCTCCCGTTCCTGATTATCGGCGATGGAACGTCCGATGTAGCTGATGTACAAATAATCCTGCGCCGACAGCAAGGCTTCAAGGAACAGATAACGATCGTCATCACGACGGGCGCGATCGCCTTTTAAACGATGATATTGCATTAAATCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATAc                                                                                                                                             <  1:761603/251‑1 (MQ=255)                                                                                             ccgGCAGATTTTCCACGATGTAATCCAACAACTGACTCACCAGCACCGACGGCTCCCGTTCCTGATTATCGGCGATGGAACGTCCGATGTAGCTGATGTACAAATAATCCTGCGCCGACAGCAAGGCTTCAAGGAACAGATAACGATCGTCATCACGACGGGCGCGATCGCCTTTTAAACGATGATATTGCATTAAATCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGc                                                                                                                                        <  1:33677/251‑1 (MQ=255)                                                                                                         ccACGATGTAATCCAACAACTGACTCACCAGCACCGACGGCTCCCGTTCCTGATTATCGGCGATGGAACGTCCGATGTAGCTGATGTACAAATAATCCTGCGCCGACAGCAAGGCTTCAAGGAACAGATAACGATCGTCATCACGACGGGCGCGATCGCCTTTTAAACGATGATATTGCATTAAATCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAActa                                                                                                                              >  1:724864/1‑247 (MQ=255)                                                                                                             gATGTAATCCAACAACTGACTCACCAGCACCGACGGCTCCCGTTCCTGATTATCGGCGATGGAACGTCCGATGTAGCTGATGTACAAATAATCCTGCGCCGACAGCAAGGCTTCAAGGAACAGATAACGATCGTCATCACGACGGGCGCGATCGCCTTTTAAACGATGATATTGCATTAAATCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGa                                                                                                                        >  1:27081/1‑251 (MQ=255)                                                                                                              aTGTAATCCAACAACTGACTCACCAGCACCGACGGCTCCCGTTCCTGATTATCGGCGATGGAACGTCCGATGTAGCTGATGTACAAATAATCCTGCGCCGACAGCAAGGCTTCAAGGAACAGATAACGATCGTCATCACGACGGGCGCGATCGCCTTTTAAACGATGATATTGCATTAAATCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGaa                                                                                                                       >  1:523683/1‑251 (MQ=255)                                                                                                              aTGTAATCCAACAACTGACTCACCAGCACCGACGGCTCCCGTTCCTGATTATCGGCGATGGAACGTCCGATGTAGCTGATGTACAAATAATCCTGCGCCGACAGCAAGGCTTCAAGGAACAGATAACGATCGTCATCACGACGGGCGCGATCGCCTTTTAAACGATGATATTGCATTAAATCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGaa                                                                                                                       >  1:372735/1‑251 (MQ=255)                                                                                                                         cAACTGACTCACCAGCACCGACGGCTCCCGTTCCTGATTATCGGCGATGGAACGTCCGATGTAGCTGATGTACAAATAATCCTGCGCCGACAGCAAGGCTTCAAGGAACAGATAACGATCGTCATCACGACGGGCGCGATCGCCTTTTAAACGATGATATTGCATTAAATCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAgc                                                                                                            <  1:891037/251‑1 (MQ=255)                                                                                                                            cTGACTCACCAGCACCGACGGCTCCCGTTCCTGATTATCGGCGATGGAACGTCCGATGTAGCTGATGTACAAATAATCCTGCGCCGACAGCAAGGCTTCAAGGAACAGATAACGATCGTCATCACGACGGGCGCGATCGCCTTTTAAACGATGATATTGCATTAAATCGAAACTGTTCGGCGCttgttgacg                                                                                                                                                                    <  1:315327/192‑4 (MQ=255)                                                                                                                                  cACCAGCACCGACGGCTCCCGTTCCTGATTATCGGCGATGGAACGTCCGATGTAGCTGATGTACAAATAATCCTGCGCCGACAGCAAGGCTTCAAGGAACAGATAACGATCGTCATCACGACGGGCGCGATCGCCTTTTAAACGATGATATTGCATTAAATCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAAc                                                                                                                                >  1:529927/1‑222 (MQ=255)                                                                                                                                         aCCGACGGCTCCCGTTCCTGATTATCGGCGATGGAACGTCCGATGTAGCTGATGTACAAATAATCCTGCGCCGACAGCAAGGCTTCAAGGAACAGATAACGATCGTCATCACGACGGGCGCGATCGCCTTTTAAACGATGATATTGCATTAAATCGAAACTGTTCGgcgc                                                                                                                                                                             <  1:779839/170‑1 (MQ=255)                                                                                                                                              cGGCTCCCGTTCCTGATTATCGGCGATGGAACGTCCGATGTAGCTGATGTACAAATAATCCTGCGCCGACAGCAAGGCTTCAAGGAACAGATAACGATCGTCATCACGACGGGCGCGATCGCCTTTTAAACGATGATATTGCATTAAATCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACaaa                                                                                       >  1:816794/1‑251 (MQ=255)                                                                                                                                                gCTCCCGTTCCTGATTATCGGCGATGGAACGTCCGATGTAGCTGATGTACAAATAATCCTGCGCCGACAGCAAGGCTTCAAGGAACAGATAACGATCGTCATCACGACGGGCGCGATCGCCTTTTAAACGATGATATTGCATTAAATCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACa                                                                                         <  1:528612/247‑1 (MQ=255)                                                                                                                                                   cccGTTCCTGATTATCGGCGATGGAACGTCCGATGTAGCTGATGTACAAATAATCCTGCGCCGACAGCAAGGCTTCAAGGAACAGATAACGATCGTCATCACGACGGGCGCGATCGCCTTTTAAACGATGATATTGCATTAAATCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAAtt                                                                                   >  1:671082/1‑250 (MQ=255)                                                                                                                                                         ccTGATTATCGGCGATGGAACGTCCGATGTAGCTGATGTACAAATAATCCTGCGCCGACAGCAAGGCTTCAAGGAACAGATAACGATCGTCATCACGACGGGCGCGATCGCCTTTTAAACGATGATATTGCATTAAATCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTg                                                                            >  1:525708/1‑251 (MQ=255)                                                                                                                                                                         ggAACGTCCGATGTAGCTGATGTACAAATAATCCTGCGCCGACAGCAAGGCTTCAAGGAACAGATAACGATCGTCATCACGACGGGCGCGATCGCCTTTTAAACGATGATATTGCATTAAATCGAAACTGTTCGGCGCttgttgac                                                                                                                                                                     >  1:256777/2‑144 (MQ=255)                                                                                                                                                                         ggAACGTCCGATGTAGCTGATGTACAAATAATCCTGCGCCGACAGCAAGGCTTCAAGGAACAGATAACGATCGTCATCACGACGGGCGCGATCGCCTTTTAAACGATGATATTGCATTAAATCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCAc                                                                                >  1:332082/2‑231 (MQ=255)                                                                                                                                                                          gAACGTCCGATGTAGCTGATGTACAAATAATCCTGCGCCGACAGCAAGGCTTCAAGGAACAGATAACGATCGTCATCACGACGGGCGCGATCGCCTTTTAAACGATGATATTGCATTAAATCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCggt                                                            <  1:252549/250‑3 (MQ=255)                                                                                                                                                                              gtccGATGTAGCTGATGTACAAATAATCCTGCGCCGACAGCAAGGCTTCAAGGAACAGATAACGATCGTCATCACGACGGGCGCGATCGCCTTTTAAACGATGATATTGCATTAAATCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAAt                                                                                                                 >  1:785030/3‑193 (MQ=255)                                                                                                                                                                              gtccGATGTAGCTGATGTACAAATAATCCTGCGCCGACAGCAAGGCTTCAAGAAACAGATAACGATCGTCATCACGACGGGCGCGATCGCCTTTTAAACGATGATATTGCATTAAATCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCggtagtga                                                       >  1:800790/3‑244 (MQ=255)                                                                                                                                                                                ccGATGTAGCTGATGTACAAATAATCCTGCGCCGACAGCAAGGCTTCAAGGAACAGATAACGATCGTCATCACGACGGGCGCGATCGCCTTTTAAACGATGATATTGCATTAAATCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCggtagtgatt                                                     <  1:151457/251‑10 (MQ=255)                                                                                                                                                                                   aTGTAGCTGATGTACAAATAATCCTGCGCCGACAGCAAGGCTTCAAGGAACAGATAACGATCGTCATCACGACGGGCGCGATCGCCTTTTAAACGATGATATTGCATTGAATCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAAt                                                                                                                 <  1:707300/188‑1 (MQ=255)                                                                                                                                                                                   aTGTAGCTGATGTACAAATAATCCTGCGCCGACAGCAAGGCTTCAAGGAACAGATAACGATCGTCATCACGACGGGCGCGATCGCCTTTTAAACGATGATATTGCATTAAATCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGcagcaa                                                                                               >  1:646663/1‑206 (MQ=255)                                                                                                                                                                                   aTGTAGCTGATGTACAAATAATCCTGCGCCGACAGCAAGGCTTCAAGGAACAGATAACGATCGTCATCACGACGGGCGCGATCGCCTTTTAAACGATGATATTGCATTAAATCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACaaaaa                                                                                     >  1:701300/1‑216 (MQ=255)                                                                                                                                                                                   aTGTAGCTGATGTACAAATAATCCTGCGCCGACAGCAAGGCTTCAAGGAACAGATAACGATCGTCATCACGACGGGCGCGATCGCCTTTTAAACGATGATATTGCATTAAATCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCgg                                                  >  1:66252/1‑251 (MQ=255)                                                                                                                                                                                       aGCTGATGTACAAATAATCCTGCGCCGACAGCAAGGCTTCAAGGAACAGATAACGATCGTCATCACGACGGGCGCGATCGCCTTTTAAACGATGATATTGCATTAAATCGAAACTGTTCGGCGCttgttgacg                                                                                                                                                                    >  1:69145/1‑130 (MQ=255)                                                                                                                                                                                                 cAAATAATCCTGCGCCGACAGCAAGGCTTCAAGGAACAGATAACGATCGTCATCACGACGGGCGCGATCGCCTTTTAAACGATGATATTGCATTAAATCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACg                                    >  1:48057/1‑251 (MQ=255)                                                                                                                                                                                                  aaaTAATCCTGCGCCGACAGCAAGGCTTCAAGGAACAGATAACGATCGTCATCACGACGGGCGCGATCGCCTTTTAAACGATGATATTGCATTAAATCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACg                                    >  1:764899/1‑250 (MQ=255)                                                                                                                                                                                                       atCCTGCGCCGACAGCAAGGCTTCAAGGAACAGATAACGATCGTCATCACGACGGGCGCGATCGCCTTTTAAACGATGATATTGCATTAAATCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCattgt                              >  1:12777/1‑249 (MQ=255)                                                                                                                                                                                                               ccGACAGCAAGGCTTCAAGGAACAGATAACGATCGTCATCACGACGGGCGCGATCGCCTTTTAAACGATGATATTGCATTAAATCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGAc                       <  1:750877/250‑1 (MQ=255)                                                                                                                                                                                                                 gACAGCAAGGCTTCAAGGAACAGATAACGATCGTCATCACGACGGGCGCGATCGCCTTTTAAACGATGATATTGCATTAAATCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCATCCAACGCATTGTTCATGACAt                     >  1:15892/1‑250 (MQ=255)                                                                                                                                                                                                                         ggCTTCAAGGAACAGATAACGATCGTCATCACGACGGGCGCGATCGCCTTTTAAACGATGATATTGCATTAAATCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATctg                  <  1:461484/245‑3 (MQ=255)                                                                                                                                                                                                                           cTTCAAGGAACAGATAACGATCGTCATCACGACGGGCGCGATCGCCTTTTAAACGATGATATTGCATTAAATCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACcaca          <  1:267484/251‑1 (MQ=255)                                                                                                                                                                                                                             tCAAGGAACAGATAACGATCGTCATCACGACGGGCGCGATCGCCTTTTAAACGATGATATTGCATTAAATCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGcagc                                                                                                 <  1:118674/162‑3 (MQ=255)                                                                                                                                                                                                                              cAAGGAACAGATAACGATCGTCATCACGACGGGCGCGATCGCCTTTTAAACGATGATATTGCATTAAATCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATcc       <  1:206365/251‑1 (MQ=255)                                                                                                                                                                                                                                    aCAGATAACGATCGTCATCACGACGGGCGCGATCGCCTTTTAAACGATGATATTGCATTAAATCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAAc                                     >  1:497950/1‑215 (MQ=255)                                                                                                                                                                                                                                    aCAGATAACGATCGTCATCACGACGGGCGCGATCGCCTTTTAAACGATGATATTGCATTAAATCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATCCACGcc  >  1:248450/1‑250 (MQ=255)                                                                                                                                                                                                                                       gATAACGATCGTCATCACGACGGGCGCGATCGCCTTTTAAACGATGATATTGCATTAAATCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACaaaaa                                                                                     >  1:469601/1‑164 (MQ=255)                                                                                                                                                                                                                                       gATAACGATCGTCATCACGACGGGCGCGATCGCCTTTTAAACGATGATATTGCATTAAATCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGAc                       >  1:93721/1‑226 (MQ=255)                                                                                                                                                                                                                                           aCGATCGTCATCACGACGGGCGCGATCGCCTTTTAAACGATGATATTGCATTAAATCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTtcc                                           >  1:312832/1‑202 (MQ=255)                                                                                                                                                                                                                                              aTCGTCATCACGACGGGCGCGATCGCCTTTTAAACGATGATATTGCATTAAATCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACaaa                                                                                       >  1:852904/1‑155 (MQ=255)                                                                                                                                                                                                                                              aTCGTCATCACGACGGGCGCGATCGCCTTTTAAACGATGATATTGCATTAAATCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGATTTTCCTCCAACGCATTGTTCATGACATCTGCCACCACATcc       >  1:74318/1‑235 (MQ=255)                                                                                                                                                                                                                                                 gTCATCACGACGGGCGCGATCGCCTTTTAAACGATGATATTGCATTAAATCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGGTAGTGATTCGGAt                                                <  1:88655/191‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                       TTTTTCAAAGTTTTTACGACTGAACACGGTCATGCCATGTTGTTGAATCAGTCGTTCGCGCCACTTGAGATTTCCCGATGGGGTATTTTCCGCCGGCAGATTTTCCACGATGTAATCCAGCAACTGACTCACCAGCACCGACGGCTCCCGCTCCTGATTATCGGCAATAGAACGACCGATGTAGCTGATGTACAAATAATCCTGCGCCGACAGCAAGGCTTCAAGGAACAGATAACGATCGTCATCGCGACGGGCGCGATCGCCTTTTAAGCGGTGATATTGCATTAAATCGAAACTGTTCGGCGCTTGTTGGCGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACCACTTTGAACGGAATGGAGCGCATCGGCAACAATGTACAAAAATTCACTTTGCCGGCGAGGAAGCGATAATGGTTCGGATTTTCCTCCAACGCATTATTCATGACATCCGCCACCACATCCACGCC  >  1/1831329‑1831805

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑