Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,831,497	A→G	intergenic (+49597/+3887)	unknown → / ← unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,831,497	0	A	G	98.6%	198.0 / NA	70	intergenic (+49597/+3887)	unknown/unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (39/30);  minor base C (1/0);  total (40/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGGTTTGATTTACCTGCGAATTTGCCAAAGGTAGCCACTGTTGCGCGAAAGATTGCATAAAAGTGCAGTCGTTTTTTTCAAAGTTTTTACGACTGAACACGGTCATGCCATGTTGTTGAATCAGTCGTTCGCGCCACTTGAGATTTCCCGATGGGGTATTTTCCGCCGGCAGATTTTCCACGATGTAATCCAGCAACTGACTCACCAGCACCGACGGCTCCCGCTCCTGATTATCGGCAATAGAACGACCGATGTAGCTGATGTACAAATAATCCTGCGCCGACAGCAAGGCTTCAAGGAACAGATAACGATCGTCATCGCGACGGGCGCGATCGCCTTTTAAGCGGTGATATTGCATTAAATCGAAACTGTTCGGCGCTTGTTGGCGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACCACTTTGAACGGAATGGAGCGCATCGGCAACAATGTACAAAAATTCACTTTG  >  1/1831256‑1831731                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                           aGGTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAGATTGCATAAAAGTGCGGTCGTTTTTTTCAAAGTTTTTGCGACTGAACGCCGTCATGCCATGTTGTTGAATCAGTCGTTCGCGCCACTTGAGATTTCCCGCTGGGGTATTTTCTGCCGGCAGATTTTCCACGATGTAATCCAACAACTGACTCACCAGCACCGACGGCTCCCGTTCCTGATTATCGGCGATGGAACGTCCg                                                                                                                                                                                                                                   >  1:527006/1‑251 (MQ=255)  ggTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAGATTGCATAAAAGTGCGGTCGTTTTTTTCAAAGTTTTTGCGACTGAACGCCGTCATGCCATGTTGTTGAATCAGTCGTTCGCGCCACTTGAGATTTCCCGCTGGGGTATTTTCTGCCGGCAGATTTTCCACGATGTAATCCAACAACTGACTCACCAGCACCGACGGCTCCCGTTCCTGATTATCGGCGATGGAACGTCCGa                                                                                                                                                                                                                                  >  1:704603/1‑251 (MQ=255)  ggTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAGATTGCATAAAAGTGCGGTCGTTTTTTTCAAAGTTTTTGCGACTGAACGCCGTCATGCCATGTTGTTGAATCAGTCGTTCGCGCCACTTGAGATTTCCCGCTGGGGTATTTTCTGCCGGCAGATTTTCCACGATGTAATCCAACAACTGACTCACCAGCACCGACGGCTCCCGTTCCTGATTATCGGCGATGGAACGTCCGa                                                                                                                                                                                                                                  >  1:847548/1‑251 (MQ=255)        aTTTGCCTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAGATTGCATAAAAGTGCGGTCGTTTTTTTCAAAGTTTTTGCGACTGAACGCCGTCATGCCATGTTGTTGAATCAGTCGTTCGCGCCACTTGAGATTTCCCGCTGGGGTATTTTCTGCCGGCAGATTTTCCACGATGTAATCCAACAACTGACTCACCAGCACCGACGGCTCCCGTTCCTGATTATCGGCGATGGAACGTCCGATGTAGc                                                                                                                                                                                                                            <  1:879774/251‑1 (MQ=255)          ttgccTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAGATTGCATAAAAGTGCGGTCGTTTTTTTCAAAGTTTTTGCGACTGAACGCCGTCATGCCATGTTGTTGAATCAGTCGTTCGCGCCACTTGAGATTTCCCGCTGGGGTATTTTCTGCCGGCAGATTTTCCACGATGTAATCCAACAACTGACTCACCAGCACCGACGGCTCCCGTTCCTGATTATCGGCGATGGAACGTCCGATGTAGCTg                                                                                                                                                                                                                          <  1:40145/248‑1 (MQ=255)             ccTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAGATTGCATAAAAGTGCGGTCGTTTTTTTCAAAGTTTTTGCGACTGAACGCCGTCATGCCATGTTGTTGAATCAGTCGTTCGCGCCACTTGAGATTTCCCGCTGGGGTATTTTCTGCCGGCAGATTTTCCACGATGTAATCCAACAACTGACTCACCAGCACCGACGGCTCCCGTTCCTGATTATCGGCGATGGAACGTCCGATGTAGCTGATg                                                                                                                                                                                                                       <  1:41794/251‑1 (MQ=255)                     tttGCCAAAGGCAGCCACTGTTGCGCGAAAGATTGCATAAAAGTGCGGTCGTTTTTTTCAAAGTTTTTGCGACTGAACGCCGTCATGCCATGTTGTTGAATCAGTCGTTCGCGCCACTTGAGATTTCCCGCTGGGGTATTTTCTGCCGGCAGATTTTCCACGATGTAATCCAACAACTGACTCACCAGCACCGACGGCTCCCGTTCCTGATTATCGGCGATGGAACGTCCGATGTAGCTGATGTACAaata                                                                                                                                                                                                               <  1:155836/251‑1 (MQ=255)                        gCCAAAGGCAGCCACTGTTGCGCGAAAGATTGCATAAAAGTGCGGTCGTTTTTTTCAAAGTTTTTGCGACTGAACGCCGTCATGCCATGTTGTTGAATCAGTCGTTCGCGCCACTTGAGATTTCCCGCTGGGGAATTTTCTGCCGGCAGATTTTCCACGATGTAATCCAACAACTGACTCACCAGCACCGACGGCTCCCGTTCCTGATTATCGGCGATGGAACGTCCGATGTAGCTGATGTACAaataat                                                                                                                                                                                                             >  1:898826/1‑250 (MQ=255)                           aaaGGCAGCCACTGTTGCGCGAAAGATTGCATAAAAGTGCGGTCGTTTTTTTCAAAGTTTTTGCGACTGAACGCCGTCATGCCATGTTGTTGAATCAGTCGTTCGCGCCACTTGAGATTTCCCGCTGGGGTATTTTCTGCCGGCAGATTTTCCACGATGTAATCCAACAACTGACTCACCAGCACCGACGGCTCCCGTTCCTGATTATCGGCGATGGAACGTCCGATGTAGCTGATGTACAAATAATc                                                                                                                                                                                                            >  1:631029/1‑248 (MQ=255)                              ggcaGCCACTGTTGCGCGAAAGATTGCATAAAAGTGCGGTCGTTTTTTTCAAAGTTTTTGCGACTGAACGCCGTCATGCCATGTTGTTGAATCAGTCGTTCGCGCCACTTGAGATTTCCCGCTGGGGTATTTTCTGCCGGCAGATTTTCCACGATGTAATCCAACAACTGACTCACCAGCACCGACGGCTCCCGTTCCTGATTATCGGCGATGGAACGTCCGATGTAGCTGATGTACAAATAATCCTgcg                                                                                                                                                                                                       >  1:478886/4‑250 (MQ=255)                                caGCCACTGTTGCGCGAAAGATTGCATAAAAGTGCGGTCGTTTTTTTCAAAGTTTTTGCGACTGAACGCCGTCATGCCATGTTGTTGAATCAGTCGTTCGCGCCACTTGAGATTTCCCGCTGGGGTATTTTCTGCCGGCAGATTTTCCACGATGTAATCCAACAACTGACTCACCAGCACCGACGGCTCCCGTTCCTGATTATCGGCGATGGAACGTCCGATGTAGCTGATGTACAAATAATCCTGCGCCg                                                                                                                                                                                                    >  1:98473/2‑251 (MQ=255)                                 aGCCACTGTTGCGCGAAAGATTGCATAAAAGTGCGGTCGTTTTTTTCAAAGTTTTTGCGACTGAACGCCGTCATGCCATGTTGTTGAATCAGTCGTTCGCGCCACTTGAGATTTCCCGCTGGGGTATTTTCTGCCGGCAGATTTTCCACGATGTAATCCAACAACTGACTCACCAGCACCGACGGCTCCCGTTCCTGATTATCGGCGATGGAACGTCCGATGt                                                                                                                                                                                                                               <  1:532898/223‑1 (MQ=255)                                   ccACTGTTGCGCGAAAGATTGCATAAAAGTGCGGTCGTTTTTTTCAAAGTTTTTGCGACTGAACGCCGTCATGCCATGTTGTTGAATCAGTCGTTCGCGCCACTTGAGATTTCCCGCTGGGGTATTTTCTGCCGGCAGATTTTCCACGATGTAATCCAACAACTGACTCACCAGCACCGACGGCTCCCGTTCCTGATTATCGGCGATGGAACGTCCGATGTAGCTGATGTACAAATAATCCTGCGCCGACa                                                                                                                                                                                                 >  1:231105/1‑251 (MQ=255)                                      cTGTTGCGCGAAAGATTGCATAAAAGTGCGGTCGTTTTTTTCAAAGTTTTTGCGACTGAACGCCGTCATGCCATGTTGTTGAATCAGTCGTTCGCGCCACTTGAGATTTCCCGCTGGGGTATTTTCTGCCGGCAGATTTTCCACGATGTAATCCAACAACTGACTCACCAGCACCGACGGCTCCCGTTCCTGATTATCGGCGATGGAACGTCCGATGTAGCTGATGTACaaa                                                                                                                                                                                                                 <  1:497541/232‑1 (MQ=255)                                      cTGTTGCGCGAAAGATTGCATAAAAGTGCGGTCGTTTTTTTCAAAGTTTTTGCGACTGAACGCCGTCATGCCATGTTGTTGAATCAGTCGTTCGCGCCACTTGAGATTTCCCGCTGGGGTATTTTCTGCCGGCAGATTTTCCACGATGTAATCCAACAACTGACTCACCAGCACCGACGGCTCCCGTTCCTGATTATCGGCGATGGAACGTCCGATGTAGCTGATGTACAAATAATCCTGCGCCGACAGCa                                                                                                                                                                                              >  1:257666/1‑251 (MQ=255)                                          tGCGCGAAAGATTGCATAAAAGTGCGGTCGTTTTTTTCAAAGTTTTTGCGACTGAACGCCGTCATGCCATGTTGTTGAATCAGTCGTTCGCGCCACTTGAGATTTCCCGCTGGGGTATTTTCTGCCGGCAGATTTTCCACGATGTAATCCAACAACTGACTCACCAGCACCGACGGCTCCCGTTCCTGATTATCGGCGATGGAACGTCCGATGTAGCTGATGTACAAATAATCCTGCGCCGACAGCAAGGc                                                                                                                                                                                          <  1:862253/251‑1 (MQ=255)                                           gcgcGAAAGATTGCATAAAAGTGCGGTCGTTTTTTTCAAAGTTTTTGCGACTGAACGCCGTCATGCTATGTTGTTGAATTAGTCGATCGCGCCACTTGAGATTTCCCGCTGGGGTATTTTCTGCCGGCAGATTTTCCACGATGTAATCCAACAACTGGCTCACCAGCACCGACGGCCCCCGTTCCTGATTATCGGCGATCGCATGTCCGATGTAGCTGATGTACAAATAATcc                                                                                                                                                                                                           >  1:485305/1‑233 (MQ=255)                                            cgcgAAAGATTGCATAAAAGTGCGGTCGTTTTTTTCAAAGTTTTTGCGACTGAACGCCGTCATGCCATGTTGTTGAATCAGTCGTTCGCGCCACTTGAGATTTCCCGCTGGGGTATTTTCTGCCGGCAGATTTTCCACGATGTAATCCAACAACTGACTCACCAGCACCGACGGCTCCCGTTCCTGATTATCGGCGATGGAACGTCCGATGTAGCTGATGTACAAATAATcc                                                                                                                                                                                                           >  1:848777/1‑232 (MQ=255)                                                  aGATTGCATAAAAGTGCGGTCGTTTTTTTCAAAGTTTTTGCGACTGAACGCCGTCATGCCATGTTGTTGAATCAGTCGTTCGCGCCACTTGAGATTTCCCGCTGGGGTATTTTCTGCCGGCAGATTTTCCACGATGTAATCCAACAACTGACTCACCAGCACCGACGGCTCCCGTTCCTGATTATCGGCGATGGAACGTCCGATGTAGCTGATGTACAAATAATCCTGCGCCGACAGCAAGGCTTCAAgg                                                                                                                                                                                   >  1:249722/1‑250 (MQ=255)                                                     ttGCATAAAAGTGCGGTCGTTTTTTTCAAAGTTTTTGCGACTGAACGCCGTCATGCCATGTTGTTGAATCAGTCGTTCGCGCCACTTGAGATTTCCCGCTGGGGTATTTTCTGCCGGCAGATTTTCCACGATGTAATCCAACAACTGACTCACCAGCACCGACGGCTCCCGTTCCTGATTATCGGCGATGGAACGTCCGATGTAGCTGATGTACAAATAATCCTGCGCCGACAg                                                                                                                                                                                                <  1:562584/234‑1 (MQ=255)                                                     ttGCATAAAAGTGCGGTCGTTTTTTTCAAAGTTTTTGCGACTGAACGCCGTCATGCCATGTTGTTGAATCAGTCGTTCGCGCCACTTGAGATTTCCCGCTGGGGTATTTTCTGCCGGCAGATTTTCCACGATGTAATCCAACAACTGACTCACCAGCACCGACGGCTCCCGTTCCTGATTATCGGCGATGGAACGTCCGATGTAGCTGATGTACAAATAATCCTGCGCCGACAGCAAGGCTTCAAGGAACa                                                                                                                                                                               >  1:109521/1‑251 (MQ=255)                                                      tGCATAAAAGTGCGGTCGTTTTTTTCAAAGTTTTTGCGACTGAACGCCGTCATGCCATGTTGTTGAATCAGTCGTTCGCGCCACTTGAGATTTCCCGCTGGGGTATTTTCTGCCGGCAGATTTTCCACGATGTAATCCAACAACTGACTCACCAGCACCGACGGCTCCCGTTCCTGATTATCGGCGATGGAACGTCCGATGTAGCTGATGTACAAATAATCCTGCGCCGACAGCAAGGCTTCAAGGAACAg                                                                                                                                                                              <  1:309185/251‑1 (MQ=255)                                                       gCATAAAAGTGCGGTCGTTTTTTTCAAAGTTTTTGCGACTGAACGCCGTCATGCCATGTTGTTGAATCAGTCGTTCGCGCCACTTGAGATTTCCCGCTGGGGTATTTTCTGCCGGCAGATTTTCCACGATGTAATCCAACAACTGACTCACCAGCACCGACGGCTCCCGTTCCTGATTATCGGCGATGGAACGTCCGATGTAGCTGATGTACAAATAATCCTGCGCCGACAGCAAGGCTTCAAGGCACAGa                                                                                                                                                                             >  1:604735/1‑251 (MQ=255)                                                       gCATAAAAGTGCGGTCGTTTTTTTCAAAGTTTTTGCGACTGAACGCCGTCATGCCATGTTGTTGAATCAGTCGTTCGCGCCACTTGAGATTTCCCGCTGGGGTATTTTCTGCCGGCAGATTTTCCACGATGTAATCCAACAACTGACTCACCAGCACCGACGGCTCCCGTTCCTGATTATCGGCGATGGAACGTCCGATGTAGCTGATGTACAAATAATCCTGCGCCGACAGCAAGGCTTCAAGGAACAGa                                                                                                                                                                             >  1:876884/1‑251 (MQ=255)                                                       gCATAAAAGTGCGGTCGTTTTTTTCAAAGTTTTTGCGACTGAACGCCGTCATGCCATGTTGTTGAATCAGTCGTTCGCGCCACTTGAGATTTCCCGCTGGGGTATTTTCTGCCGGCAGATTTTCCACGATGTAATCCAACAACTGACTCACCAGCACCGACGGCTCCCGTTCCTGATTATCGGCGATGGAACGTCCGATGTAGCTGATGTACAAATAATCCTGCGCCGACAGCAAGGCTTCAAGGAACAGa                                                                                                                                                                             >  1:309648/1‑251 (MQ=255)                                                               gTGCGGTCGTTTTTTTCAAAGTTTTTGCGACTGAACGCCGTCATGCCATGTTGTTGAATCAGTCGTTCGCGCCACTTGAGATTTCCCGCTGGGGTATTTTCTGCCGGCAGATTTTCCACGATGTAATCCAACAACTGACTCACCAGCACCGACGGCTCCCGTTCCTGATTATCGGCGATGGAACGTCCGATGTAGCTGATGTACAAATAATCCTGCGCCGACAGCAAGGCTTCAAGGAACAGATAACGAt                                                                                                                                                                      >  1:300565/1‑250 (MQ=255)                                                                tgcggTCGTTTTTTTCAAAGTTTTTGCGACTGAACGCCGTCATGCCATGTTGTTGAATCAGTCGTTCGCGCCACTTGAGATTTCCCGCTGGGGTATTTTCTGCCGGCAGATTTTCCACGATGTAATCCAACAACTGACTCACCAGCACCGACGGCTCCCGTTCCTGATTATCGGCGATGGAACGTCCGATGTAGCTGATGTACAAATAATCCTGCGCCGACAGCAAGGCTTCAAGGAACAGATAACGAt                                                                                                                                                                      <  1:791166/245‑1 (MQ=255)                                                                tgcggTCGTTTTTTTCAAAGTTTTTGCGACTGAACGCCGTCATGCCATGTTGTTGAATCAGTCGTTCGCGCCACTTGAGATTTCCCGCTGGGGTATTTTCTGCCGGCAGATTTTCCACGATGTAATCCAACAACTGACTCACCAGCACCGACGGCTCCCGTTCCTGATTATCGGCGATGGAACGTCCGATGTAGCTGATGTACAAATAATCCTGCGCCGACAGCAAGGCTTCAAGGAACAGATAACGAt                                                                                                                                                                      <  1:183703/245‑1 (MQ=255)                                                                          tttttCAAAGTTTTTGCGACTGAACGCCGTCATGCCATGTTGTTGAATCAGTCGTTCGCGCCACTTGAGATTTCCCGCTGGGGTATTTTCTGCCGGCAGATTTTCCACGATGTAATCCAACAACTGACTCACCAGCACCGACGGCTCCCGTTCCTGATTATCGGCGATGGAACGTCCGATGTAGCTGATGTACAAATAATCCTGCGCCGACAGCAAGGCTTCAAGGAACAGATAACGATCGTCATCACGAc                                                                                                                                                          <  1:48330/251‑1 (MQ=255)                                                                             ttCAAAGTTTTTGCGACTGAACGCCGTCATGCCATGTTGTTGAATCAGTCGTTCGCGCCACTTGAGATTTCCCGCTGGGGTATTTTCTGCCGGCAGATTTTCCACGATGTAATCCAACAACTGACTCACCAGCACCGACGGCTCCCGTTCCTGATTATCGGCGATGGAACGTCCGATGTAGCTGATGTACAAATAATCCTGCGCCGACAGCAAGGCTTCAAGGAACAGATAACGATCGTCATCACGACggg                                                                                                                                                       <  1:448391/251‑1 (MQ=255)                                                                                    tttttGCGACTGAACGCCGTCATGCCATGTTGTTGAATCAGTCGTTCGCGCCACTTGAGATTTCCCGCTGGGGTATTTTCTGCCGGCAGATTTTCCACGATGTAATCCAACAACTGACTCACCAGCACCGACGGCTCCCGTTCCTGATTATCGGCGATGGAACGTCCGATGTAGCTGATGTACAAATAATCCTGCGCCGACAGCAAGGCTTCAAGGAACAGATAACGATCGTCATCACGACGGGCGCGATc                                                                                                                                                <  1:470865/251‑1 (MQ=255)                                                                                     ttttGCGACTGAACGCCGTCATGCCATGTTGTTGAATCAGTCGTTCGCGCCACTTGAGATTTCCCGCTGGGGTATTTTCTGCCGGCAGATTTTCCACGATGTAATCCAACAACTGACTCACCAGCACCGACGGCTCCCGTTCCTGATTATCGGCGATGGAACGTCCGATGTAGCTGATGTACAAATAATCCTGCGCCGACAGCAAGGCTTCAAGGAACAGATAACGATCGTCATCACGACGGGCGCGATCg                                                                                                                                               >  1:613964/1‑251 (MQ=255)                                                                                              tgaacgccgTCATGCCATGTTGTTGAATCAGTCGTTCGCGCCACTTGAGATTTCCCGCTGGGGTATTTTCTGCCGGCAGATTTTCCACGATGTAATCCAACAACTGACTCACCAGCACCGACGGCTCCCGTTCCTGATTATCGGCGATGGAACGTCCGATGTAGCTGATGTACAAATAATCCTGCGCCGACAGCAAGGCTTCAAGGAACAGATAACGATCGTCATCACGACGGGCGCGATCGCCTTTTaaa                                                                                                                                      <  1:328640/243‑2 (MQ=255)                                                                                               gaacgccgTCATGCCATGTTGTTGAATCAGTCGTTCGCGCCACTTGAGATTTCCCGCTGGGGTATTTTCTGCCGGCAGATTTTCCACGATGTAATCCAACAACTGACTCACCAGCACCGACGGCTCCCGTTCCTGATTATCGGCGATGGAACGTCCGATGTAGCTGATGTACAAATAATCCTGCGCCGACAGCAAGGCTTCAAGGAAc                                                                                                                                                                                >  1:562185/8‑208 (MQ=255)                                                                                                      gTCATGCCATGTTGTTGAATCAGTCGTTCGCGCCGCTTGAGATTTCCCGCTGGGGTATTTTCTGCCGGCAGATTTTCCACGATGTAATCCAACAACTGACTCACCAGCACCGACGGCTCCCGTTCCTGATTATCGGCGATGGAACGTCCGATGTAGCTGATGTACAAATAATCCTGCGCCGACAGCAAGGCTTCAAgg                                                                                                                                                                                   <  1:662272/198‑1 (MQ=255)                                                                                                      gTCATGCCATGTTGTTGAATCAGTCGTTCGCGCCACTTGAGATTTCCCGCTGGGGTATTTTCTGCCGGCAGATTTTCCACGATGTAATCCAACAACTGACTCACCAGCACCGACGGCTCCCGTTCCTGATTATCGGCGATGGAACGTCCGATGTAGCTGATGTACAAATAATCCTGCGCCGACAGCAAGGCTTCAAGGAACAGATAACGATCGTCATCACGACGGGCGCGATCGCCTTTTAAACGATGata                                                                                                                              >  1:727960/1‑251 (MQ=255)                                                                                                       tCATGCCATGTTGTTGAATCAGTCGTTCGCGCCACTTGAGATTTCCCGCTGGGGTATTTTCTGCCGGCAGATTTTCCACGATGTAATCCAACAACTGACTCACCAGCACCGACGGCTCCCGTTCCTGATTATCGGCGATGGAACGTCCGATGTAGCTGATGTACAAATAATCCTGCGCCGACAGCAAGGCTTCAAGGAACAGATAACGATCGTCATCACGACGGGCGCGATCGCCTTTTAAACGATGatat                                                                                                                             <  1:810107/251‑1 (MQ=255)                                                                                                         aTGCCATGTTGTTGAATCAGTCGTTCGCGCCACTTGAGATTTCCCGCTGGGGTATTTTCTGCCGGCAGATTTTCCACGATGTAATCCAACAACTGACTCACCAGCACCGACGGCTCCCGTTCCTGATTATCGGCGATGGAACGTCCGATGTAGCTGATGTACAAATAATCCTGCGCCGACAGCAAGGCTTCAAGGAACAGATAACGAt                                                                                                                                                                      >  1:526049/1‑208 (MQ=255)                                                                                                         aTGCCATGTTGTTGAATCAGTCGTTCGCGCCACTTGAGATTTCCCGCTGGGGTATTTTCTGCCGGCAGATTTTCCACGATGTAATCCAACAACTGACTCACCAGCACCGACGGCTCCCGTTCCTGATTATCGGCGATGGAACGTCCGATGTAGCTGATGTACAAATAATCCTGCGCCGACAGCAAGGCTTCAAGGAACAGATAACGATCGTCATCACGACGGGCGCGATCGCCTTTTAAACGATGATATTg                                                                                                                           >  1:512700/1‑251 (MQ=255)                                                                                                            ccATGTTGTTGAATCAGTCGTTCGCGCCACTTGAGATTTCCCGCTGGGGTATTTTCTGCCGGCAGATTTTCCACGATGTAATCCAACAACTGACTCACCAGCACCGACGGCTCCCGTTCCTGATTATCGGCGATGGAACGTCCGATGTAGCTGATGTACAAATAATCCTGCGCCGACAGCAAGGCTTCAAGGAACAGATAACGATCGTCATCACGACGGGCGCGATCGCCTTTTAAACGATGATATTGCAt                                                                                                                        <  1:532992/251‑1 (MQ=255)                                                                                                              aTGTTGTTGAATCAGTCGTTCGCGCCACTTGAGATTTCCCGCTGGGGTATTTTCTGCCGGCAGATTTTCCACGATGTAATCCAACAACTGACTCACCAGCACCGACGGCTCCCGTTCCTGATTATCGGCGATGGAACGTCCGATGTAGCTGATGTACAAATAATCCTGCGCCGACAGCAAGGCTTCAAGGAACAGATAACGATCGTCATCACGACGGgcg                                                                                                                                                     >  1:10409/1‑220 (MQ=255)                                                                                                                gttgttGAATCAGTCGTTCGCGCCACTTGAGATTTCCCGCTGGGGTATTTTCTGCCGGCAGATTTTCCACGATGTAATCCAACAACTGACTCACCAGCACCGACGGCTCCCGTTCCTGATTATCGGCGATGGAACGTCCGATGTAGCTGATGTACAAATAATCCTGCGCCGACAGCAAGGCTTCAAGGAACAGATAACGATCGTCATCACGACGGGCGCGATCGCCTTTTAAACGATGATATTGCATTaaa                                                                                                                    >  1:666731/1‑251 (MQ=255)                                                                                                                 ttgttgAATCAGTCGTTCGCGCCACTTGAGATTTCCCGCTGGGGTATTTTCTGCCGGCAGATTTTCCACGATGTAATCCAACAACTGACTCACCAGCACCGACGGCTCCCGTTCCTGATTATCGGCGATGGAACGTCCGATGTAGCTGATGTACAAATAATCCTGCGCCGACAGCAAGGCTTCAAGGAACAGATAACGATCGTCATCACGACGGGCGCGATCGCCTTTTAAACGATGATATTGCATTAAAt                                                                                                                   <  1:685163/251‑1 (MQ=255)                                                                                                                   gttgAATCAGTCGTTCGCGCCACTTGAGATTTCCCGCTGGGGTATTTTCTGCCGGCAGATTTTCCACGATGTAATCCAACAACTGACTCACCAGCACCGACGGCTCCCGTTCCTGATTATCGGCGATGGAACGTCCGATGTAGCTGATGTACAAATAATCCTGCGCCGACAGCAAGGCTTCAAgg                                                                                                                                                                                   >  1:538290/1‑185 (MQ=255)                                                                                                                      gAATCAGTCGTTCGCGCCACTTGAGATTTCCCGCTGGGGTATTTTCTGCCGGCAGATTTTCCACGATGTAATCCAACAACTGACTCACCAGCACCGACGGCTCCCGTTCCTGATTATCGGCGATGGAACGTCCGATGTAGCTGATGTACAAATAATCCTGCGCCGACAGCAAGGCTTCAAGGAACAGATAACGATCGTCATCACGACGGGCGCGATCGCCTTTTAAACGATGATATTGCATTAAATCGaaa                                                                                                              >  1:696121/1‑251 (MQ=255)                                                                                                                       aaTCAGTCGTTCGCGCCACTTGAGATTTCCCGCTGGGGTATTTTCTGCCGGCAGATTTTCCACGATGTAATCCAACAACTGACTCACCAGCACCGACGGCTCCCGTTCCTGATTATCGGCGATGGAACGTCCGATGTAGCTg                                                                                                                                                                                                                          <  1:835099/142‑1 (MQ=255)                                                                                                                            gTCGTTCGCGCCACTTGAGATTTCCCGCTGGGGTATTTTCTGCCGGCAGATTTTCCACGATGTAATCCAACAACTGACTCACCAGCACCGACGGCTCCCGTTCCTGATTATCGGCGATGGAACGTCCGATGTAGCTGATGTACAAATAATCCTGCGCCGACAGCAAGGCTTCAAGGAACAGATAACGATCGTCATCACGACGGGCGCGATCGCCTTTTAAACGATGatat                                                                                                                             >  1:73391/1‑230 (MQ=255)                                                                                                                                 tCGCGCCACTTGAGATTTCCCGCTGGGGTATTTTCTGCCGGCAGATTTTCCACGATGTAATCCAACAACTGACTCACCAGCACCGACGGCTCCCGTTCCTGATTATCGGCGATGGAACGTCCGATGTAGCTGATGTACAAATAATCCTGCGCCGACAGCAAGGCTTCAAGGAACAGATAACGATCGTCATCACGACGGGCGCGATCGCCTTTTAAACGATGATATTGCATTAAATCGAAACTGTTCGgcgc                                                                                                   <  1:272560/251‑1 (MQ=255)                                                                                                                                      ccACTTGAGATTTCCCGCTGGGGTATTTTCTGCCGGCAGATTTTCCACGATGTAATCCAACAACTGACTCACCAGCACCGACGGCTCCCGTTCCTGATTATCGGCGATGGAACGTCCGATGTAGCTGATGTACAAATAATCCTGCGCCGACAGCAAGGCTTCAAGGAACAGATAACGATCGTCATCACGACGGGCGCGATCGCCTTTTAAACGATGATATTGCATTAAATCGAAACTGTTCGGCGCttgtt                                                                                              >  1:100251/1‑251 (MQ=255)                                                                                                                                       cACTTGAGATTTCCCGCTGGGGTATTTTCTGCCGGCAGATTTTCCACGATGTAATCCAACAACTGACTCACCAGCACCGACGGCTCCCGTTCCTGATTATCGGCGATGGAACGTCCGATGTAGCTGATGTACAAATAATcc                                                                                                                                                                                                           <  1:743503/141‑1 (MQ=255)                                                                                                                                        aCTTGAGATTTCCCGCTGGGGTATTTTCTGCCGGCAGATTTTCCACGATGTAATCCAACAACTGACTCACCAGCACCGACGGCTCCCGTTCCTGATTATCGGCGATGGAACGTCCGATGTAGCTGATGTACAAATAATCCTGCGCCGACAGCAAGGCTTCAAGGAACAGATAACGATCGTCATCACGACGGGCGCGATCGCCTTTTAAACGATGATATTGCATTAAATCGAAACTGTTCGGCGCttgttga                                                                                            >  1:242367/1‑250 (MQ=255)                                                                                                                                             agaTTTCCCGCTGGGGTATTTTCTGCCGGCAGATTTTCCACGATGTAATCCAACAACTGACTCACCAGCACCGACGGCTCCCGTTCCTGATTATCGGCGATGGAACGTCCGATGTAGCTGATGTACAAATAATCCTGCGCCGACAGCAAGGCTTCAAGGAACAGATAACGATCg                                                                                                                                                                    <  1:799944/174‑1 (MQ=255)                                                                                                                                                 ttCCCGCTGGGGTATTTTCTGCCGGCAGATTTTCCACGATGTAATCCAACAACTGACTCACCAGCACCGACGGCTCCCGTTCCTGATTATCGGCGATGGAACGTCCGATGTAGCTGATGTACAAATAATCCTGCGCCGACAGCAAGGCTTCAAGGAACAGATAACGATCGTCATCACGACGGGCGCGATCGCCTTTTAAACGATGATATTGCATTAAATCGAAACTGTTCGGCGCTTGTTGACGCGGGTAt                                                                                   <  1:310672/251‑1 (MQ=255)                                                                                                                                                         ggggTATTTTCTGCCGGCAGATTTTCCACGATGTAATCCAACAACTGACTCACCAGCACCGACGGCTCCCGTTCCTGATTATCGGCGATGGAACGTCCGATGTAGCTGATGTACAAATAATCCTGCGCCGACAGCAAGGCTTCAAGGAACAGATAACGATCGTCATCACGACGGGCGCGATCGCCTTTTAAACGATGATATTGCATTAAATCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCcgt                                                                            >  1:534461/1‑250 (MQ=255)                                                                                                                                                         ggggTATTTTCTGCCGGCAGATTTTCCACGATGTAATCCAACAACTGACTCACCAGCACCGACGGCTCCCGTTCCTGATTATCGGCGATGGAACGTCCGATGTAGCTGATGTACAAATAATCCTGCGCCGACAGCAAGGCTTCAAGGAACAGATAACGATCGTCATCACGACGGGCGCGATCGCCTTTTAAACGATGATATTGCATTAAATCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCcgt                                                                            >  1:256208/1‑250 (MQ=255)                                                                                                                                                          gggTATTTTCTGCCGGCAGATTTTCCACGATGTAATCCAACAACTGACTCACCAGCACCGACGGCTCCCGTTCCTGATTATCGGCGATGGAACGTCCGATGTAGCTGATGTACAAATAATCCTGCGCCGACAGCAAGGCTTCAAGGAACAGATAACGATCGTCATCACGACGGGCGCGATCGCCTTTTAAACGATGATATTGCATTAAATCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCcgtcg                                                                          >  1:158779/1‑251 (MQ=255)                                                                                                                                                                 ttCCGCCGGCAGATTTTCCACGATGTAATCCAACAACTGACTCACCAGCACCGACGGCTCCCGTTCCTGATTATCGGCGATGGAACGTCCGATGTAGCTGATGTACAAATAATCCTGCGCCGACAGCAAGGCTTCAAGGAACAGATAACGATCGTCATCACGACGGGCGCGATCGCCTTTTAAACGATGATATTGCATTAAATCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATAc                                                                   <  1:761603/251‑1 (MQ=255)                                                                                                                                                                      ccgGCAGATTTTCCACGATGTAATCCAACAACTGACTCACCAGCACCGACGGCTCCCGTTCCTGATTATCGGCGATGGAACGTCCGATGTAGCTGATGTACAAATAATCCTGCGCCGACAGCAAGGCTTCAAGGAACAGATAACGATCGTCATCACGACGGGCGCGATCGCCTTTTAAACGATGATATTGCATTAAATCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGc                                                              <  1:33677/251‑1 (MQ=255)                                                                                                                                                                                  ccACGATGTAATCCAACAACTGACTCACCAGCACCGACGGCTCCCGTTCCTGATTATCGGCGATGGAACGTCCGATGTAGCTGATGTACAAATAATCCTGCGCCGACAGCAAGGCTTCAAGGAACAGATAACGATCGTCATCACGACGGGCGCGATCGCCTTTTAAACGATGATATTGCATTAAATCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAActa                                                    >  1:724864/1‑247 (MQ=255)                                                                                                                                                                                      gATGTAATCCAACAACTGACTCACCAGCACCGACGGCTCCCGTTCCTGATTATCGGCGATGGAACGTCCGATGTAGCTGATGTACAAATAATCCTGCGCCGACAGCAAGGCTTCAAGGAACAGATAACGATCGTCATCACGACGGGCGCGATCGCCTTTTAAACGATGATATTGCATTAAATCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGa                                              >  1:27081/1‑251 (MQ=255)                                                                                                                                                                                       aTGTAATCCAACAACTGACTCACCAGCACCGACGGCTCCCGTTCCTGATTATCGGCGATGGAACGTCCGATGTAGCTGATGTACAAATAATCCTGCGCCGACAGCAAGGCTTCAAGGAACAGATAACGATCGTCATCACGACGGGCGCGATCGCCTTTTAAACGATGATATTGCATTAAATCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGaa                                             >  1:372735/1‑251 (MQ=255)                                                                                                                                                                                       aTGTAATCCAACAACTGACTCACCAGCACCGACGGCTCCCGTTCCTGATTATCGGCGATGGAACGTCCGATGTAGCTGATGTACAAATAATCCTGCGCCGACAGCAAGGCTTCAAGGAACAGATAACGATCGTCATCACGACGGGCGCGATCGCCTTTTAAACGATGATATTGCATTAAATCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGaa                                             >  1:523683/1‑251 (MQ=255)                                                                                                                                                                                                  cAACTGACTCACCAGCACCGACGGCTCCCGTTCCTGATTATCGGCGATGGAACGTCCGATGTAGCTGATGTACAAATAATCCTGCGCCGACAGCAAGGCTTCAAGGAACAGATAACGATCGTCATCACGACGGGCGCGATCGCCTTTTAAACGATGATATTGCATTAAATCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAgc                                  <  1:891037/251‑1 (MQ=255)                                                                                                                                                                                                     cTGACTCACCAGCACCGACGGCTCCCGTTCCTGATTATCGGCGATGGAACGTCCGATGTAGCTGATGTACAAATAATCCTGCGCCGACAGCAAGGCTTCAAGGAACAGATAACGATCGTCATCACGACGGGCGCGATCGCCTTTTAAACGATGATATTGCATTAAATCGAAACTGTTCGGCGCttgttgacg                                                                                          <  1:315327/192‑4 (MQ=255)                                                                                                                                                                                                           cACCAGCACCGACGGCTCCCGTTCCTGATTATCGGCGATGGAACGTCCGATGTAGCTGATGTACAAATAATCCTGCGCCGACAGCAAGGCTTCAAGGAACAGATAACGATCGTCATCACGACGGGCGCGATCGCCTTTTAAACGATGATATTGCATTAAATCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAAc                                                      >  1:529927/1‑222 (MQ=255)                                                                                                                                                                                                                  aCCGACGGCTCCCGTTCCTGATTATCGGCGATGGAACGTCCGATGTAGCTGATGTACAAATAATCCTGCGCCGACAGCAAGGCTTCAAGGAACAGATAACGATCGTCATCACGACGGGCGCGATCGCCTTTTAAACGATGATATTGCATTAAATCGAAACTGTTCGgcgc                                                                                                   <  1:779839/170‑1 (MQ=255)                                                                                                                                                                                                                       cGGCTCCCGTTCCTGATTATCGGCGATGGAACGTCCGATGTAGCTGATGTACAAATAATCCTGCGCCGACAGCAAGGCTTCAAGGAACAGATAACGATCGTCATCACGACGGGCGCGATCGCCTTTTAAACGATGATATTGCATTAAATCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACaaa             >  1:816794/1‑251 (MQ=255)                                                                                                                                                                                                                         gCTCCCGTTCCTGATTATCGGCGATGGAACGTCCGATGTAGCTGATGTACAAATAATCCTGCGCCGACAGCAAGGCTTCAAGGAACAGATAACGATCGTCATCACGACGGGCGCGATCGCCTTTTAAACGATGATATTGCATTAAATCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACa               <  1:528612/247‑1 (MQ=255)                                                                                                                                                                                                                            cccGTTCCTGATTATCGGCGATGGAACGTCCGATGTAGCTGATGTACAAATAATCCTGCGCCGACAGCAAGGCTTCAAGGAACAGATAACGATCGTCATCACGACGGGCGCGATCGCCTTTTAAACGATGATATTGCATTAAATCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAAtt         >  1:671082/1‑250 (MQ=255)                                                                                                                                                                                                                                  ccTGATTATCGGCGATGGAACGTCCGATGTAGCTGATGTACAAATAATCCTGCGCCGACAGCAAGGCTTCAAGGAACAGATAACGATCGTCATCACGACGGGCGCGATCGCCTTTTAAACGATGATATTGCATTAAATCGAAACTGTTCGGCGCTTGTTGACGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACTACTCTGAACGGAATAGAGCGCATCGGCAGCAATGTACAAAAATTCACTTTg  >  1:525708/1‑251 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                           AGGTTTGATTTACCTGCGAATTTGCCAAAGGTAGCCACTGTTGCGCGAAAGATTGCATAAAAGTGCAGTCGTTTTTTTCAAAGTTTTTACGACTGAACACGGTCATGCCATGTTGTTGAATCAGTCGTTCGCGCCACTTGAGATTTCCCGATGGGGTATTTTCCGCCGGCAGATTTTCCACGATGTAATCCAGCAACTGACTCACCAGCACCGACGGCTCCCGCTCCTGATTATCGGCAATAGAACGACCGATGTAGCTGATGTACAAATAATCCTGCGCCGACAGCAAGGCTTCAAGGAACAGATAACGATCGTCATCGCGACGGGCGCGATCGCCTTTTAAGCGGTGATATTGCATTAAATCGAAACTGTTCGGCGCTTGTTGGCGCGGGTATTCGCCGTCGTTCATACCGAGCAGACAAACCACTTTGAACGGAATGGAGCGCATCGGCAACAATGTACAAAAATTCACTTTG  >  1/1831256‑1831731

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑