Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,831,287	T→C	intergenic (+49387/+4097)	unknown → / ← unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,831,287	0	T	C	100.0%	221.8 / NA	77	intergenic (+49387/+4097)	unknown/unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (40/37);  total (40/37)

AAATCCGCATGAATGGCGTATTTTTCCAAGGCATTATCCAAGGCGAAATTTTCGCTGTCGGCAATGCTTTCTTCCTGTTCGGAGAAATTCACGCCAAGGCGTTTTTCAAAGAAAAATTTCACCGGATTTTTCACAAACGCCACCAAGCGGGAAAACTCAATTTCATTTTCCGGCTCCGCATCCGTCATCATCGGCTGAATAAAATCCTGCAAGGTTTGATTTACCTGCGAATTTGCCAAAGGTAGCCACTGTTGCGCGAAAGATTGCATAAAAGTGCAGTCGTTTTTTTCAAAGTTTTTACGACTGAACACGGTCATGCCATGTTGTTGAATCAGTCGTTCGCGCCACTTGAGATTTCCCGATGGGGTATTTTCCGCCGGCAGATTTTCCACGATGTAATCCAGCAACTGACTCACCAGCACCGACGGCTCCCGCTCCTGATTATCGGCAATAGAACGACCGATGTAGCTGATGTACAAATAATC  >  1/1831045‑1831529                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                   aaaTCCGCATGAATGGTGTATTTTTCCAAGGCATTATCCAAGGCGAAATTTTCGCTATCGGCAATGCTTTCTTCCTGTTCGGAGAAATTCACGCCAAGGCGTTTTTCAAAGAAAAATTTCACCGGATTTTTCACAAACGCCACCAAGCGGGAAAACTCGATTTCTTCTTCCGGCTCCGCATCCGCCATCATCGGCTGAATAAAATCCTGCAAGGTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCCACTg                                                                                                                                                                                                                                            <  1:525848/251‑1 (MQ=255)  aaTCCGCATGAATGGCGTATTTTTCCAAGGCATTATCCAAGGCGAAATTTTCGCTATCGGCAATGCTTTCTTCCTGTTCGGAGAAATTCACGCCAAGGCGTTTTTCAAAGAAAAATTTCACCGGATTTTTCACAAACGCCACCAAGCGGGAAAACTCGATTTCTTCTTCCGGCTCCGCATCCGCCATCATCGGCTGAATAAAATCCTGCAAGGTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCTACTGt                                                                                                                                                                                                                                           <  1:258587/251‑1 (MQ=255)  aaTCCGCATGAATGGCGTATTTTTCCAAGGCATTATCCAAGGCGAAATTTTCGCTATCGGCAATGCTTTCTTCCTGTTCGGAGAAATTCACGCCAAGGCGTTTTTCAAAGAAAAATTTCACCGGATTTTTCACAAACGCCACCAAGCGGGAAAACTCGATTTCTTCTTCCGGCTCCGCATCCGCCATCATCGGCTGAATAAAATCCTGCAAGGTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCCACTGt                                                                                                                                                                                                                                           <  1:342022/251‑1 (MQ=255)            tgacaagtgtttttCCAAGGCATTATCCAAGGCGAAATTTTCGCTATCGGCAATGCTTTCTTCCTGTTCGGAGAAATTCACGCCAAGGCGTTTTTCAAAGAAAAATTTCACCGGATTTTTCACAAACGCCACCAAGCGGGAAAACTCGATTTCTTCTTCCGGCTCCGCATCCGCCATCATCGGCTGAATAAAATCCTGCAAGGTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAg                                                                                                                                                                                                                                 <  1:829681/242‑1 (MQ=255)            aaTGGCGTATTTTTCCAAGGCATTATCCAAGGCGAAATTTTCGCTATCGGCAATGCTTTCTTCCTGTTCGGAGAAATTCACGCCAAGGCGTTTTTCAAAGAAAAATTTCACCGGATTTTTCACAAACGCCACCAAGCGGGAAAACTCGATTTCTTCTTCCGGCTCCGCATCCGCCATCATCGGCTGAATAAAATCCTGCAAGGTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAg                                                                                                                                                                                                                                 <  1:191840/251‑1 (MQ=255)            aaTGGCGTATTTTTCCAAGGCATTATCCAAGGCGAAATTTTCGCTATCGGCAATGCTTTCTTCCTGTTCGGAGAAATTCACGCCAAGGCGTTTTTCAAAGAAAAATTTCACCGGATTTTTCACAAACGCCACCAAGCGGGAAAACTCGATTTCTTCTTCCGGCTCCGCATCCGCCATCATCGGCTGAATAAAATCCTGCAAGGTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAg                                                                                                                                                                                                                                 <  1:431620/251‑1 (MQ=255)              tGGCGTATTTTTCCAAGGCATTATCCAAGGCGAAATTTTCGCTATCGGCAATGCTTTCTTCCTGTTCGGAGAAATTCACGCTAAGGCGTTTTTCAAAGAAAAATTTCACCGGATTTTTCACAAACGCCACCAAGCGGGAAAACTCGATTTCTTCTTCCGGCTCCGCATCCGCCATCATCGGCTGAATAAAATCCTGCAAGGTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAGAt                                                                                                                                                                                                                               <  1:440580/251‑1 (MQ=255)              tGGCGTATTTTTCCAAGGCATTATCCAAGGCGAAATTTTCGCTATCGGCAATGCTTTCTTCCTGTTCGGAGAAATTCACGCCAAGGCGTTTTTCAAAGAAAAATTTCACCGGATTTTTCACAAACGCCACCAAGCGGGAAAACTCGATTTCTTCTTCCGGCTCCGCATCCGCCATCATCGGCTGAATAAAATCCTGCAAGGTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAGAt                                                                                                                                                                                                                               <  1:521263/251‑1 (MQ=255)              tGGCGTATTTTTCCAAGGCATTATCCAAGGCGAAATTTTCGCTATCGGCAATGCTTTCTTCCTGTTCGGAGAAATTCACGCCAAGGCGTTTTTCAAAGAAAAATTTCACCGGATTTTTCACAAACGCCACCAAGCGGGAAAACTCGATTTCTTCTTCCGGCTCCGCATCCGCCATCATCGGCTGAATAAAATCCTGCAAGGTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAGAt                                                                                                                                                                                                                               <  1:623965/251‑1 (MQ=255)                  gTATTTTTCCAAGGCATTATCCAAGGCGAAATTTTCGCTATCGGCAATGCTTTCTTCCTGTTCGGAGAAATTCACGCCAAGGCGTTTTTCAAAGAAAAATTTCACCGGATTTTTCACAAACGCCACCAAGCGGGAAAACTCGATTTCTTCTTCCGGCTCCGCATCCGCCCTCATCGGCTGAATAAAATCCTGCAAGGTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAGATTGCa                                                                                                                                                                                                                           >  1:455530/1‑251 (MQ=255)                  gTATTTTTCCAAGGCATTATCCAAGGCGAAATTTTCGCTATCGGCAATGCTTTCTTCCTGTTCGGAGAAATTCACGCCAAGGCGTTTTTCAAAGAAAAATTTCACCGGATTTTTCACAAACGCCACCAAGCGGGAAAACTCGATTTCTTCTTCCGGCTCCGCATCCGCCATCATCGGCTGAATAAAATCTTGCAAGTTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAGATTg                                                                                                                                                                                                                             >  1:867636/1‑249 (MQ=255)                   tATTTTTCCAAGGCATTATCCAAGGCGAAATTTTCGCTATCGGCAATGCTTTCTTCCTGTTCGGAGAAATTCACGCCAAGGCGTTTTTCAAAGAAAAATTTCACCGGATTTTTCACAAACGCCACCAAGCGGGAAAACTCGATTTCTTCTTCCGGCTCCGCATCCGCCATCATCGGCTGAATAAAATCCTGCAAGGTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAGATTGCAt                                                                                                                                                                                                                          <  1:687923/251‑1 (MQ=255)                        ttCCAAGGCATTATCCAAGGCGAAATTTTCGCTATCGGCAATGCTTTCTTCCTGTTCGGAGAAATTCACGCCAAGGCGTTTTTCAAAGAAAAATTTCACCGGATTTTTCACAAACGCCACCAAGCGGGAAAACTCGATTTCTTCTTCCGGGTCAGCATCCGCCATCATCGGCTGAATAAAATCCTGCAAGGTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAGATTGCATAAAAg                                                                                                                                                                                                                     <  1:383112/251‑1 (MQ=255)                        ttCCAAGGCATTATCCAAGGCGAAATTTTCGCTATCGGCAATGCTTTCTTCCTGTTCGGAGAAATTCACGCCAAGGCGTTTTTCAAAGAAAAATTTCACCGGATTTTTCACAAACGCCACCAAGCGGGAAAACTCGATTTCTTCTTCCGGCTCCGCATCCGCCATCATCGGCTGAATAAAATCCTGCAAGGTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAGATTGCATAAAAg                                                                                                                                                                                                                     <  1:853074/251‑1 (MQ=255)                        ttCCAAGGCATTATCCAAGGCGAAATTTTCGCTATCGGCAATGCTTTCTTCCTGTTCGGAGAAATTCACGCCAAGGCGTTTTTCAAAGAAAAATTTCACCGGATTTTTCACAAACGCCACCAAGCGGGAAAACTCGATTTCTTCTTCCGGCTCCGCATCCGCCATCATCGGCTGAATAAAATCCTGCAAGGTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAGATTGCATAAAAg                                                                                                                                                                                                                     >  1:780869/1‑251 (MQ=255)                        ttCCAAGGCATTATCCAAGGCGAAATTTTCGCTATCGGCAATGCTTTCTTCCTGTTCGGAGAAATTCACGCCAAGGCGTTTTTC‑AAGAAAAATTTCACCGGATTTTTCACAAACGCCACCAAGCGGGAAAACTCGATTTCTTCTTCCGGCTCCGCATCCGCCATCATCGGCTGAATAAAATCCTGCAAGGTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAGATTGCATAAAAGt                                                                                                                                                                                                                    >  1:545110/1‑251 (MQ=255)                               gCATTATCCAAGGCGAAATTTTCGCTATCGGCAATGCTTTCTTCCTGTTCGGAGAAATTCACGCCAAGGCGTTTTTCAAAGAAAAATTTCACCGGATTTTTCACAAACGCCACCAAGCGGGAAAACTCGATTTCTTCTTCCGGCTCCGCATCCGCCATCATCGGCTGAATAAAATCCTGCAAGGTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAGATTGCATAAAAGTGcggt                                                                                                                                                                                                               >  1:868613/1‑247 (MQ=255)                               gCATTATCCAAGGCGAAATTTTCGCTATCGGCAATGCTTTCTTCCTGTTCGGAGAAATTCACGCCAAGGCGTTTTTCAAAGAAAAATTTCACCGGATTTTTCACAAACGCCACCAAGCGGGAAAACTCGATTTCTTCTTCCGGCTCCGCATCCGCCATCATCGGCTGAATAAAATCCTGCAAGGTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAGATTGCATAAAAGTGcggt                                                                                                                                                                                                               >  1:396076/1‑247 (MQ=255)                                                              cAATGCTTTCTTCCTGTTCGGAGAAATTCACGCCAAGGCGTTTTTCAAAGAAAAATTTCACCGGATTTTTCACAAACGCCACCAAGCGGGAAAACTCGATTTCTTCTTCCGGCTCCGCATCCGCCATCATCGGCTGAATAAAATCCTGCAAGGTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAGATTGCATAAAAGTGCGGTCGTTTTTTTCAAAGTTTTTGCGACTGAacgc                                                                                                                                                                                <  1:561396/250‑3 (MQ=255)                                                                aTGCTTTCTTCCTGTTCGGAGAAATTCACGCCAAGGCGTTTTTCAAAGAAAAATTTCACCGGATTTTTCACAAACGCCACCAAGCGGGAAAACTCGATTTCTTCTTCCGGCTCCGCATCCGCCATCATCGGCTGAATAAAATCCTGCAAGGTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAGATTGCATAAAAGTGCGGTCGTTTTTTTCAAAGTTTTTGCGACTGAacgccgt                                                                                                                                                                             <  1:4525/251‑6 (MQ=255)                                                                  gCTTTCTTCCTGTTCGGAGAAATTCACGCCAAGGCGTTTTTCAAAGAAAAATTTCACCGGATTTTTCACAAACGCCACCAAGCGGGAAAACTCGATTTCTTCTTCCGGCTCCGCATCCGCCATCATCGGCTGAATAAAATCCTGCAAGGTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAGATTGCATAAAAg                                                                                                                                                                                                                     >  1:94837/1‑209 (MQ=255)                                                                  gCTTTCTTCCTGTTCGGAGAAATTCACGCCAAGGCGTTTTTCAAAGAAAAATTTCACCGGATTTTTCACAAACGCCACCAAGCGGGAAAACTCGATTTCTTCTTCCGGCTCCGCATCCGCCATCATCGGCTGAATAAAATCCTGCAAGGTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAGATTGCATAAAAGTGCGGTCGTTTTTTTCAAAGTTTTTGCGAc                                                                                                                                                                                       <  1:447899/239‑1 (MQ=255)                                                                  gCTTTCTTCCTGTTCGGAGAAATTCACGCCAAGGCGTTTTTCAAAGAAAAATTTCACCGGATTTTTCACAAACGCCACCAAGCGGGAAAACTCGATTTCTTCTTCCGGCTCCGCATCCGCCATCATCGGCTGAATAAAATCCTGCAAGGTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAGATTGCATAAAAGTGCGGTCGTTTTTTTCAAAGTTTTTGCGACTGAacgccgtca                                                                                                                                                                           >  1:805949/1‑244 (MQ=255)                                                                  gCTTTCTTCCTGTTCGGAGAAATTCACGCCAAGGCGTTTTTCAAAGAAAAATTTCACCGGATTTTTCACAAACGCCACCAAGCGGGAAAACTCGATTTCTTCTTCCGGCTCCGCATCCGCCATCATCGGCTGAATAAAATCCTGCAAGGTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAGATTGCATAAAAGTGCGGTCGTTTTTTTCAAAGTTTTTGCGACTGAacgccgtca                                                                                                                                                                           >  1:242350/1‑244 (MQ=255)                                                                  gCTTTCTTCCTGTTCGGAGAAATTCACGCCAAGGCGTTTTTCAAAGAAAAATTTCACCGGATTTTTCACAAACGCCACCAAGCGGGAAAACTCGATTTCTTCTTCCGGCTCCGCATCCGCCATCATCGGCTGAATAAAATCCTGCAAGGTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAGATTGCATAAAAGTGCGGTCGTTTTTTTCAAAGTTTTTGCGACTGAacgccgtca                                                                                                                                                                           >  1:399884/1‑244 (MQ=255)                                                                  gCTTTCTTCCTGTTCGGAGAAATTCACGCCAAGGCGTCTTTCAAAGAAAAATTTCACCGGATTTTTCACAAACGCCACCAAGCGGGAAAACTCGATTTCTTCTTCCGGCTCCGCATCCGCCATCATCGGCTGAATAAAATCCTGCAAGGTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAGATTGCATAAAAGTGCGGTCGTTTTTTTCAAAGCTTTTGCGACTGAacgccgtca                                                                                                                                                                           >  1:881413/1‑244 (MQ=255)                                                                   cTTTCTTCCTGTTCGGAGAAATTCACGCCAAGGCGTTTTTCAAAGAAAAATTTCACCGGATTTTTCACAAACGCCACCAAGCGGGAAAACTCGATTTCTTCTTCCGGCTCCGCATCCGCCATCATCGGCTGAATAAAATCCTGCAAGGTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAGATTGCATAAAAGTGCGGTCGTTTTTTTCAAAGTTTTTGCGACTGAacgccgtca                                                                                                                                                                           >  1:122550/1‑243 (MQ=255)                                                                          ccTGTTCGGAGAAATTCACGCCAAGGCGTTTTTCAAAGAAAAATTTCACCGGATTTTTCACAAACGCCACCAAGCGGGAAAACTCGATTTCTTCTTCCGGCTCCGCATCCGCCATCATCGGCTGAATAAAATCCTGCAAGGTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAGATTGCATAAAAGTGCGGTCGTTTTTTTCAAAGTTTTTGCGACTGAACGCCGTCATGCCAtgt                                                                                                                                                                   <  1:305001/251‑1 (MQ=255)                                                                                      aaTTCACGCCAAGGCGTTTTTCAAAGAAAAATTTCACCGGATTTTTCACAAACGCCACCAAGCGGGAAAACTCGATTTCTTCTTCCGGCTCCGCATCCGCCATCATCGGCTGAATAAAATCCTGCAAGGTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAGATTGCATAAAAGTGCGGTCGTTTTTTTCAAAGTTTTTGCGACTGAACGCCGTCATGCCATGTTGTTGAATCAGt                                                                                                                                                       >  1:219212/1‑251 (MQ=255)                                                                                       aTTCACGCCAAGGCGTTTTTCAAAGAAAAATTTCACCGGATTTTTCACAAACGCCACCAAGCGGGAAAACTCGATTTCTTCTTCCGGCTCCGCATCCGCCATCATCGGCTGAATAAAATCCTGCAAGGTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCCAc                                                                                                                                                                                                                                              >  1:771495/1‑163 (MQ=255)                                                                                        ttCACGCCAAGGCGTTTTTCAAAGAAAAATTTCACCGGATTTTTCACAAACGCCACCAAGCGGGAAAACTCGATTTCTTCTTCCGGCTCCGCATCCGCCATCATCGGCTGAATAAAATCCTGCAAGGTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAGATTGCATAAAAGTGCGGTCGTTTTTTTCAAAGTTTTTGCGACTGAACGCCGTCATGCCATGTTGTTGAATCAGTCg                                                                                                                                                     <  1:585432/251‑1 (MQ=255)                                                                                                aaGGCGTTTTTCAAAGAAAAATTTCACCGGATTTTTCACAAACGCCACCAAGCGGGAAAACTCGATTTCTTCTTCCGGCTCCGCATCCGCCATCATCGGCTGAATAAAATCCTGCAAGGTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAGATTGCATAAAAGTGCGGTCGTTTTTTTCAAAGTTTTTGCGACTGAACGCCGTCATGCCATGTTGTTGAATCAGTCGTTCGCGcc                                                                                                                                             <  1:292541/251‑1 (MQ=255)                                                                                                  ggCGTTTTTCAAAGAAAAATTTCACCGGATTTTTCACAAACGCCACCAAGCGGGAAAACTCGATTTCTTCTTCCGGCTCCGCATCCGCCATCATCGGCTGAATAAAATCCTGCAAGGTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAGATTGCATAAAAGTGCGGTCGTTTTTTTCAAAGTTTTTGCGACTGAACGCCGTCATGCCATGTTGTTGAATCAGTCGTTCGCGCCa                                                                                                                                            >  1:104576/1‑250 (MQ=255)                                                                                                                aaaaaTTTCACCGGATTTTTCACAAACGCCACCAAGCGGGAAAACTCGATTTCTTCTTCCGGCTCCGCATCCGCCATCATCGGCTGAATAAAATCCTGCAAGGTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAGATTGCATAAAAGTGCGGTCGTTTTTTTCAAAGTTTTTGCGACTGAACGCCGTCATGCCATGTTGTTGAATCAGTCGTTCGCGCCACTTGAGATTTCCCg                                                                                                                              <  1:215289/250‑1 (MQ=255)                                                                                                                  aaaTTTCACCGGATTTTTCACAAACGCCACCAAGCGGGAAAACTCGATTTCTTCTTCCGGCTCCGCATCCGCCATCATCGGCTGAATAAAATCCTGCAAGGTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAGATTGCATAAAAGTGCGGTCGTTTTTTTCAAAGTTTTTGCGACTGAACGCCGTCATGCCATGTTGTTGAATCAGTCGTTCGCGCCACTTGAGATTTCCCg                                                                                                                              >  1:331458/1‑248 (MQ=255)                                                                                                                             gATTTTTCACAAACGCCACCAAGCGGGAAAACTCGATTTCTTCTTCCGGCTCCGCATCCGCCATCATCGGCTGAATAAAATCCTGCAAGGTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAGATTGCATAAAAGTGCGGTCGTTTTTTTCAAAGTTTTTGCGACTGAACGCCGTCATGCCATGttgttg                                                                                                                                                              >  1:346375/1‑205 (MQ=255)                                                                                                                                      caAACGCCACCAAGCGGGAAAACTCGATTTCTTCTTCCGGCTCCGCATCCGCCATCATCGGCTGAATAAAATCCTGCAAGGTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAGATTGCATAAAAGTGCGGTCGTTTTTTTCAAAGTTTTTGCGACTGAACGCCGTCATGCCATGTTGTTGAATCAGTCGTTCGCGCCACTTGAGATTTCCCGCTGGGGTATTTTCTGCCGGCAGa                                                                                                       <  1:801513/251‑1 (MQ=255)                                                                                                                                        aaCGCCACCAAGCGGGAAAACTCGATTTCTTCTTCCGGCTCCGCATCCGCCATCATCGGCTGAATAAAATCCTGCAAGGTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAGATTGCATAAAAg                                                                                                                                                                                                                     >  1:436528/1‑139 (MQ=255)                                                                                                                                            ccaccaAGCGGGAAAACTCGATTTCTTCTTCCGGCTCCGCATCCGCCATCATCGGCTGAATAAAATCCTGCAAGGTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAGATTGCATAAAAGTGCGGTCGTTTTTTTCAAAGTTTTTGCGACTGAACGCCGTCATGCCATGTTGTTGAATCAGTCGTTCGCGCCACTTGAGATTTccc                                                                                                                               >  1:629599/1‑221 (MQ=255)                                                                                                                                                 aaGCGGGAAAACTCGATTTCTTCTTCCGGCTCCGCATCCGCCATCATCGGCTGAATAAAATCCTGCAAGGTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAGATTGCATAAAAGTGCGGTCGTTTTTTTCAAAGTTTTTGCGACTGAACGCCGTCATGCCATGTTGTTGAATCAGTCGTTCGCGCCACTTGAGATTTCCCGCTGGGGTATTTTCTGCCGGCAGATTTTCCACGAt                                                                                            >  1:494918/1‑251 (MQ=255)                                                                                                                                                   gCGGGAAAACTCGATTTCTTCTTCCGGCTCCGCATCCGCCATCATCGGCTGAATAAAATCCTGCAAGGTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAGATTGCATAAAAg                                                                                                                                                                                                                     <  1:118026/128‑1 (MQ=255)                                                                                                                                                      ggAAAACTCGATTTCTTCTTCCGGCTCCGCATCCGCCATCATCGGCTGAATAAAATCCTGCAAGGTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAGATTGCATAAAAGTGCGGTCGTTTTTTTCAAAGTTTTTGCGTCTGAACGCCGTCATGCCATGTTGTTGAATCAGTCGTTCGCGCCACTTGAGATTTCCCGCTGGGGTATTTTCTGCCGGCAGATTTTCCACGATGTAAt                                                                                       >  1:691851/1‑251 (MQ=255)                                                                                                                                                            ctcgatttcttcttCCGGCTCCGCATCCGCCATCATCGGCTGAATAAAATCCTGCAAGGTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAGATTGCATAAAAGTGCGGTCGTTTTTTTCAAAGTTTTTGCGACTGAACGCCGTCATGCCATGTTGTTGAATCAGTCGTTCGCGCCACTTGAGATTTCCCGCTGGGGTATTTTCTGCCGGCAGATTTTCCACGATGTAATCCaaca                                                                                 >  1:320739/13‑248 (MQ=255)                                                                                                                                                                  ttcttcttCCGGCTCCGCATCCGCCATCATCGGCTGAATAAAATCCTGCAAGGTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAGATTGCATAAAAGTGCGGTCGTTTTTTTCAAAGTTTTTGCGACTGAACGCCGTCATGCCATGTTGTTGAATCAGTCGTTCGCGCCACTTGAGATTTCCCGCTGGGGTATTTTCTGCCGGCAGATTTTCCACGATGTAATcc                                                                                     >  1:888985/7‑241 (MQ=255)                                                                                                                                                                  ttcttcttCCGGCTCCGCATCCGCCATCATCGGCTGAATAAAATCCTGCAAGGTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAGATTGCATAAAAGTGCGGTCGTTTTTTTCAAAGTTTTTGCGACTGAACGCCGTCATGCCATGTTGTTGAATCAGTCGTTCGCGCCACTTGAGATTTCCCGCTGGGGTATTTTCTGCCGGCAGATTTTCCACGATGTAATCCAACAACTGAc                                                                           <  1:789822/245‑1 (MQ=255)                                                                                                                                                                   tcttcttCCGGCTCCGCATCCGCCATCATCGGCTGAATAAAATCCTGCAAGGTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAGATTGCATAAAAGTGCGGTCGTTTTTTTCAAAGTTTTTGCGACTGAACGCCGTCATGCCATGTTGTTGaa                                                                                                                                                            <  1:259780/164‑1 (MQ=255)                                                                                                                                                                   tcttcttCCGGCTCCGCATCCGCCATCATCGGCTGAATAAAATCCTGCAAGGTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAGATTGCATAAAAGTGCGGTCGTTTTTTTCAAAGTTTTTGCGACTGAACGCCGTCATGCCATGTTGTTGAATCAGTCGTTCGCGCCACTTGAGATTTCCCGCTGGGGTATTTTCTGCCGGCAGATTTTCCACGATGTAATCCAACAACTGACt                                                                          <  1:294862/246‑1 (MQ=255)                                                                                                                                                                        ttCCGGCTCCGCATCCGCCATCATCGGCTGAATAAAATCCTGCAAGGTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAGATTGCATaaa                                                                                                                                                                                                                       >  1:299022/1‑105 (MQ=255)                                                                                                                                                                             gCTCCGCATCCGCCATCATCGGCTGAATAAAATCCTGCAAGGTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAGATTGCATAAAAGTGCGGTCGTTTTTTTCAAAGTTTTTGCGACTGAACGCCGTCATGCCATGTTGTTGAATCAGTCGTTCGCGCCACTTGAGATTTCCCGCTGGGGTATTTTCTGCCGGCAGATTTTCCACGATGTAATCCAACAACTGACTCACCAGCAc                                                                 <  1:557777/250‑1 (MQ=255)                                                                                                                                                                                ccGCATCCGCCATCATCGGCTGAATAAAATCCTGCAAGGTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAGATTGCATAAAAGTGCGGTCGTTTTTTTCAAAGTTTTTGCGACTGAACGCCGTCATGCCATGTTGTTGAATCAGTCGTTCGCGCCACTTGAGATTTCCCGCTGGGGTATTTTCTGCCGGCAGATTTTCCACGATGTAATCCAACAACTGACTCACCAGCACCGAc                                                             <  1:287174/251‑1 (MQ=255)                                                                                                                                                                                  gCATCCGCCATCATCGGCTGAATAAAATCCTGCAAGGTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAGATTGCATAAAAGTGCGGTCGTTTTTTTCAAAGTTTTTGCGACTGAACGCCGTCATGCCATGTTGTTGAATCAGTCGTTCGCGCCACTTGAGATTTCCCGCTGGGGTATTTTCTGCCGGCAGATTTTCCACGATGTAATCCAACAACTGACTCACCAGCACCGACgg                                                           >  1:80588/1‑251 (MQ=255)                                                                                                                                                                                     tCCGCCATCATCGGCTGAATAAAATCCTGCAAGGTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAGATTGCATAAAAGTGCGGTCGTTTTTTTCAAAGTTTTTGCGACTGAACGCCGTCATGCCATGTTGTTGAATCAGTCGTTCGCGCCACTTGAGATTTCCCGCTGGGGTATTTTCTGCCGGCAGATTTTCCACGATGTAATCCAACAACTGACTCACCAGCACCGACGGCTc                                                        <  1:535380/251‑1 (MQ=255)                                                                                                                                                                                      ccgccatcatCGGCTGAATAAAATCCTGCAAGGTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAGATTGCATAAAAGTGCGGTCGTTTTTTTCAAAGTTTTTGCGACTGAACGCCGTCATGCCATGTTGTTGAATCAGTCGTTCGCGCCACTTGAGATTTCCCGCTGGGGTAttt                                                                                                                   <  1:750518/187‑1 (MQ=255)                                                                                                                                                                                           atcatcGGCTGAATAAAATCCTGCAAGGTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAGATTGCAt                                                                                                                                                                                                                          >  1:398406/1‑83 (MQ=255)                                                                                                                                                                                           atcatcGGCTGAATAAAATCCTGCAAGGTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAGATTGCATAAAAGTGCGGTCGTTTTTTTCAAAGTTTTTGCGACTGAACGCCGTCATGCCATGTTGTTGAATCAGTCGTTCGCGCCACTTGAGATTTCCCGCTGGGGTATTTTCTGCCGGCAGATTTTCCACGATGTAATCCAACAACTGACTCACCAGCACCGACGGCTCCCgtt                                                   >  1:901588/1‑248 (MQ=255)                                                                                                                                                                                           atcatcGGCTGAATAAAATCCTGCAAGGTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAGATTGCATAAAAGTGCGGTCGTTTTTTTCAAAGTTTTTGCGACTGAACGCCGTCATGCCATGTTGTTGAATCAGTCGTTCGCGCCACTTGAGATTTCCCGCTGGGGTATTTTCTGCCGGCAGATTTTCCACGATGTAATCCAACAACTGACTCACCAGCACCGACGGCTCCCg                                                     <  1:397922/248‑1 (MQ=255)                                                                                                                                                                                                  gCTGAATAAAATCCTGCAAGGTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAGATTGCATAAAAGTGCGGTCGTTTTTTTCAAAGTTTTTGCGACTGAACGCCGTCATGCCATGTTGTTGAATCAGTCGTTCGCGCCACTTGAGATTTCCCGCTGGGGTATTTTCTGCCGGCAGATTTTCCACGATGTAATCCAACAACTGACTCACCAGCACCGACGGCTCCCGTTCCTGATTa                                           >  1:5541/1‑251 (MQ=255)                                                                                                                                                                                                     gAATAAAATCCTGCAAGGTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAGATTGCATAAAAGTGCGGTCGTTTTTTTCAAAGTTTTTGCGACTGAACGCCGTCATGCCATGTTGTTGAATCAGTCGTTCGCGCCACTTGAGATTTCCCGCTGGGGTATTTTCTGCCGGCAGATTTTCCACGATGTAATcc                                                                                     <  1:728788/206‑1 (MQ=255)                                                                                                                                                                                                     gAATAAAATCCTGCAAGGTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAGATTGCATAAAAGTGCGGTCGTTTTTTTCAAAGTTTTTGCGACTGAACGCCGTCATGCCATGTTGTTGAATCAGTCGTTCGCGCCACTTGAGATTTCCCGCTGGGGTATTTTCTGCCGGCAGATTTTCCACGATGTAATCCAACAACTGACTCACCAGCACCGACGGCTCCCGTTCCTGATTATCg                                        >  1:534587/1‑251 (MQ=255)                                                                                                                                                                                                      aaTAAAATCCTGCAAGGTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAGATTGCATAAAAGTGCGGTCGTTTTTTTCAAAGTTTTTGCGACTGAACGCCGTCATGCCATGTTGTTGAATCAGTCGTTCGCGCCACTTGAGATTTCCCGCTGGGGTATTTTCTGCCGGCAGATTTTCCACGATGTAATCCAACAACTGACTCACCAGCACCGACGGCTCCCGTTCCTGATTATCgg                                       >  1:570521/1‑251 (MQ=255)                                                                                                                                                                                                       aTAAAATCCTGCAAGGTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAGATTGCATAAAAGTGCGGTCGTTTTTTTCAAAGTTTTTGCGACTGAACGCCGTCATGCCATGTTGTTGAATCAGTCGTTCGCGCCACTTGAGATTTCCCGCTGGGGTATTTTCTGCCGGCAGATTTTCCACGATGTAATCCAACAACTGACTCACCAGCACCGACGGCTCCCGTTCCTGATTATCgg                                       >  1:579779/1‑250 (MQ=255)                                                                                                                                                                                                       aTAAAATCCTGCAAGGTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAGATTGCATAAAAGTGCGGTCGTTTTTTTCAAAGTTTTTGCGACTGAACGCCGTCATGCCATGTTGTTGAATCAGTCGTTCGCGCCACTTGAGATTTCCCGCTGGGGTATTTTCTGCCGGCAGATTTTCCACGATGTAATCCAACAACTGACTCACCAGCACCGACGGCTCCCGTTCCTGATTATCgg                                       >  1:27562/1‑250 (MQ=255)                                                                                                                                                                                                          aaaTCCTGCAAGGTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAGATTGCATAAAAGTGCGGTCGTTTTTTTCAAAGTTTTTGCGACTGAACGCCGTCATGCCAGGTTGTTGAATCAGTCGTTCGCGCCACTTGAGATTTCCCGCTgggg                                                                                                                        <  1:872704/166‑1 (MQ=255)                                                                                                                                                                                                            aTCCTGCAAGGTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAGATTGCATAAAAGTGCGGTCGTTTTTTTCAAAGTTTTTGCGACTGAACGCCGTCATGCCATGTTGTTGAATCAGTCGTTCGCGCCACTTGAGATTTCCCGCTGGGGCATTTTCTGCCGGCAGATTTTCCACGATGTAATCCAACAACTGACTCACCAGCACCGACGGCTCCCGTTCCTGATTATCGGcgatgg                                 >  1:241492/1‑246 (MQ=255)                                                                                                                                                                                                                    aGGTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAGATTGCATAAAAGTGCGGTCGTTTTTTTCAAAGTTTTTGCGACTGAACGCCGTCATGCCATGTTGTTGAATCAGTCGTTCGCGCCACTTGAGATTTCCCGCTGGGGTATTTTCTGCCGGCAGATTTTCCACGATGTAATCCAACAACTGACTCACCAGCACCGACGGCTCCCg                                                     >  1:639537/1‑223 (MQ=255)                                                                                                                                                                                                                    aGGTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAGATTGCATAAAAGTGCGGTCGTTTTTTTCAAAGTTTTTGCGACTGAACGCCGTCATGCCATGTTGTTGAATCAGTCGTTCGCGCCACTTGAGATTTCCCGCTGGGGTATTTTCTGCCGGCAGATTTTCCACGATGTAATCCAACAACTGACTCACCAGCACCGACGGCTCCCGTTCCTGATTATCGGCGATGGAACGTCCg                         >  1:527006/1‑251 (MQ=255)                                                                                                                                                                                                                     ggTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAGATTGCATAAAAGTGCGGTCGTTTTTTTCAAAGTTTTTGCGACTGAACGCCGTCATGCCATGTTGTTGAATCAGTCGTTCGCGCCACTTGAGATTTCCCGCTGGGGTATTTTCTGCCGGCAGATTTTCCACGATGTAATCCAACAACTGACTCACCAGCACCGACGGCTCCCGTTCCTGATTATCGGcgatggaa                               >  1:666502/1‑237 (MQ=255)                                                                                                                                                                                                                     ggTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAGATTGCATAAAAGTGCGGTCGTTTTTTTCAAAGTTTTTGCGACTGAACGCCGTCATGCCATGTTGTTGAATCAGTCGTTCGCGCCACTTGAGATTTCCCGCTGGGGTATTTTCTGCCGGCAGATTTTCCACGATGTAATCCAACAACTGACTCACCAGCACCGACGGCTCCCGTTCCTGATTATCGGCGATGGAACGTCCGa                        >  1:704603/1‑251 (MQ=255)                                                                                                                                                                                                                     ggTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAGATTGCATAAAAGTGCGGTCGTTTTTTTCAAAGTTTTTGCGACTGAACGCCGTCATGCCATGTTGTTGAATCAGTCGTTCGCGCCACTTGAGATTTCCCGCTGGGGTATTTTCTGCCGGCAGATTTTCCACGATGTAATCCAACAACTGACTCACCAGCACCGACGGCTCCCGTTCCTGATTATCGGCGATGGAACGTCCGa                        >  1:847548/1‑251 (MQ=255)                                                                                                                                                                                                                         tGATTTGCCTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAGATTGCATAAAAGTGCGGTCGTTTTTTTCAAAGTTTTTGCGACTGAACGCCGTCATGCCATGTTGTTGAATCAGTCGTTCGCGCCa                                                                                                                                            <  1:805491/131‑1 (MQ=255)                                                                                                                                                                                                                           aTTTGCCTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAGATTGCATAAAAGTGCGGTCGTTTTTTTCAAAGTTTTTGCGACTGAACGCCGTCATGCCATGTTGTTGAATCAGTCGTTCGCGCCACTTGAGATTTCCCGCTGGGGTATTTTCTGCCGGCAGATTTTCCACGATGTAATCCAACAACTGACTCACCAGCACCGACGGCTCCCGTTCCTGATTATCGGCGATGGAACGTCCGATGTAGc                  <  1:879774/251‑1 (MQ=255)                                                                                                                                                                                                                             ttgccTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAGATTGCATAAAAGTGCGGTCGTTTTTTTCAAAGTTTTTGCGACTGAACGCCGTCATGCCATGTTGTTGAATCAGTCGTTCGCGCCACTTGAGATTTCCCGCTGGGGTATTTTCTGCCGGCAGATTTTCCACGATGTAATCCAACAACTGACTCACCAGCACCGACGGCTCCCGTTCCTGATTATCGGCGATGGAACGTCCGATGTAGCTg                <  1:40145/248‑1 (MQ=255)                                                                                                                                                                                                                               gccTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAGATTGCATAAAAGTGCGGTCGTTTTTTTCAAAGTTTTTGCGACTGAACGCCGTCATGCCATGTTGTTGAATCAGTCGTTCGCGCCACTTGAGATTTCCCGCTGGGGTATTTTCTGCCGGCAGATTTTCCACGATGTAATCCAACAACTGACTCACCAGCAc                                                                 <  1:259990/199‑1 (MQ=255)                                                                                                                                                                                                                                ccTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAGATTGCATAAAAGTGCGGTCGTTTTTTTCAAAGTTTTTGCGACTGAACGCCGTCATGCCATGTTGTTGAATCAGTCGTTCGCGCCACTTGAGATTTCCCGCTGGGGTATTTTCTGCCGGCAGATTTTCCACGATGTAATCCAACAACTGACTCACCAGCACCGACGGCTCCCGTTCCTGATTATCGGCGATGGAACGTCCGATGTAGCTGATg             <  1:41794/251‑1 (MQ=255)                                                                                                                                                                                                                                        tttGCCAAAGGCAGCCACTGTTGCGCGAAAGATTGCATAAAAGTGCGGTCGTTTTTTTCAAAGTTTTTGCGACTGAACGCCGTCATGCCATGTTGTTGAATCAGTCGTTCGCGCCACTTGAGATTTCCCGCTGGGGTATTTTCTGCCGGCAGATTTTCCACGATGTAATCCAACAACTGACTCACCAGCACCGACGGCTCCCGTTCCTGATTATCGGCGATGGAACGTCCGATGTAGCTGATGTACAaata     <  1:155836/251‑1 (MQ=255)                                                                                                                                                                                                                                           gCCAAAGGCAGCCACTGTTGCGCGAAAGATTGCATAAAAGTGCGGTCGTTTTTTTCAAAGTTTTTGCGACTGAACGCCGTCATGCCATGTTGTTGAATCAGTCGTTCGCGCCACTTGAGATTTCCCGCTGGGGAATTTTCTGCCGGCAGATTTTCCACGATGTAATCCAACAACTGACTCACCAGCACCGACGGCTCCCGTTCCTGATTATCGGCGATGGAACGTCCGATGTAGCTGATGTACAaataat   >  1:898826/1‑250 (MQ=255)                                                                                                                                                                                                                                              aaaGGCAGCCACTGTTGCGCGAAAGATTGCATAAAAGTGCGGTCGTTTTTTTCAAAGTTTTTGCGACTGAACGCCGTCATGCCATGTTGTTGAATCAGTCGTTCGCGCCACTTGAGATTTCCCGCTGGGGTATTTTCTGCCGGCAGATTTTCCACGATGTAATCCAACAACTGACTCACCAGCACCGACGGCTCCCGTTCCTGATTATCGGCGATGGAACGTCCGATGTAGCTGATGTACAAATAATc  >  1:631029/1‑248 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                   AAATCCGCATGAATGGCGTATTTTTCCAAGGCATTATCCAAGGCGAAATTTTCGCTGTCGGCAATGCTTTCTTCCTGTTCGGAGAAATTCACGCCAAGGCGTTTTTCAAAGAAAAATTTCACCGGATTTTTCACAAACGCCACCAAGCGGGAAAACTCAATTTCATTTTCCGGCTCCGCATCCGTCATCATCGGCTGAATAAAATCCTGCAAGGTTTGATTTACCTGCGAATTTGCCAAAGGTAGCCACTGTTGCGCGAAAGATTGCATAAAAGTGCAGTCGTTTTTTTCAAAGTTTTTACGACTGAACACGGTCATGCCATGTTGTTGAATCAGTCGTTCGCGCCACTTGAGATTTCCCGATGGGGTATTTTCCGCCGGCAGATTTTCCACGATGTAATCCAGCAACTGACTCACCAGCACCGACGGCTCCCGCTCCTGATTATCGGCAATAGAACGACCGATGTAGCTGATGTACAAATAATC  >  1/1831045‑1831529

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑