Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,831,203	A→G	intergenic (+49303/+4181)	unknown → / ← unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,831,203	0	A	G	100.0%	201.4 / NA	70	intergenic (+49303/+4181)	unknown/unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (35/35);  total (35/35)

ATGGCGGAGAACTCCCCGCGCGGCAACACGCCTTTGACTTTCAGACGCGCAAAAAACGCGTCGGTTTGCTGCTCGTCCGCTTCCACCAAATCCGCATGAATGGCGTATTTTTCCAAGGCATTATCCAAGGCGAAATTTTCGCTGTCGGCAATGCTTTCTTCCTGTTCGGAGAAATTCACGCCAAGGCGTTTTTCAAAGAAAAATTTCACCGGATTTTTCACAAACGCCACCAAGCGGGAAAACTCAATTTCATTTTCCGGCTCCGCATCCGTCATCATCGGCTGAATAAAATCCTGCAAGGTTTGATTTACCTGCGAATTTGCCAAAGGTAGCCACTGTTGCGCGAAAGATTGCATAAAAGTGCAGTCGTTTTTTTCAAAGTTTTTACGACTGAACACGGTCATGCCATGTTGTTGAATCAGTCGTTCGCGCCACTTGAGATTTCCCGATGGGGTATTTTCCGCCGGCAGATTTTCCACGATGTAAT  >  1/1830958‑1831444                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                  aTGGCGGAGAACTCCCCGCGCGGCAACACGCCTTTGACTTTCAGACGCGCAAAAAACGCGTCGGTTTGCTGCTCGTCTGCTTCTAACAAATCCGCATGAATGGCGTATTTTTCCAAGGCATTATCCAAGGCGAAATTTTCGCTATCGGCAATGCTTTCTTCCTGTTCGGAGAAATTCACGCCAAGGCGTTTTTCAAAGAAAAATTTCACCGGATTTTTCACAAACGCCACCAAGCGGGAAAACTCGAttt                                                                                                                                                                                                                                               >  1:741047/1‑250 (MQ=255)      ggAGAACTCCCCGCGCGGCAACACGCCTTTGACTTTCAGACGCGCAAAAAACGCGTCGGTTTGCTGCTCGTCTGCTTCTAACAAATCCGCATGAATGGCGTATTTTTCCAAGGCATTATCCAAGGCGAAATTTTCGCTATCGGCAATGCTTTCTTCCTGTTCGGAGAAATTCACGCCAAGGCGTTTTTCAAAGAAAAATTTCACCGGATTTTTCACAAACGCCACCAAGCGGGAAAACTCGATTTcttctt                                                                                                                                                                                                                                         >  1:143447/1‑246 (MQ=255)         gaACTCCCCGCGCGGCAACACGCCTTTGACTTTCAGACGCGCAAAAAACGCGTCGGTTTGCTGCTCGTCTGCTTCTAACAAATCCGCATGAATGGCGTATTTTTCCAAGGCATTATCCAAGGCGAAATTTTCGCTATCGGCAATGCTTTCTTCCTGTTCGGAGAAATTCACGCCAAGGCGTTTTTCAAAGAAAAATTTCACCGGATTTTTCACAAACGCCACCAAGCGGGAAAACTCGATTTCTTCTTCCg                                                                                                                                                                                                                                      >  1:269773/1‑251 (MQ=255)          aaCTCCCCGCGCGGCAACACGCCTTTGACTTTCAGACGCGCAAAAAACGCGTCGGTTTGCTGCTCGTCTGCTTCTAACAAATCCGCATGAATGGCGTATTTTTCCAAGGCATTATCCAAGGCGAAATTTTCGCTATCGGCAATGCTTTCTTCCTGTTCGGAGAAATTCACGCCAAGGCGTTTTTCAAAGAAAAATTTCACCGGATTTTTCACAAACGCCACCAAGCGGGAAAACTCGATTTCTTCTTCCg                                                                                                                                                                                                                                      <  1:197982/250‑1 (MQ=255)                   cgcgGCAACACGCCTTTGACTTTCAGACGCGCAAAAAACGCGTCGGTTTGCTGCTCGTCTGCTTCTAACAAATCCGCATGAATGGCGTATTTTTCCAAGGCATTATCCAAGGCGAAATTTTCGCTATCGGCAATGCTTTCTTCCTGTTCGGAGAAATTCACGCCAAGGCGTTTTTCAAAGAAAAATTTCACCGGATTTTTCACAAACGCCACCAAGCGGGAAAACTCGATTTCTTCTTCCGGCTCCGCAt                                                                                                                                                                                                                             >  1:9434/1‑250 (MQ=255)                             cGCCTTTGACTGTCAGACGCGCAAAAAACGCGTCTGTTTGCTGCTCGTCTGCTTCTAACAAATCCGCATGAATGGCGTATTTTTCCAAGGCATTATCCAAGGCGAAATTTGCGCTATCGGCAATGCTTTCTTCCTGTTCGGAGAAATTCACGCCAAGGCGTTTTTCAAAGAAAAATTTCACCGGATTTTTCACAAACGCCACCAAGCGGGAAAACACGATTTCTTCTTCCGGCTCCGCATCCGCCatcatc                                                                                                                                                                                                                  <  1:876211/251‑1 (MQ=255)                              gCCTTTGACTTTCAGACGCGCAAAAAACGCGTCGGTTTGCTGCTCGTCTGCTTCTAACAAATCCGCATGAATGGCGTATTTTTCCAAGGCATTATCCAAGGCGAAATTTTCGCTATCGGCAATGCTTTCTTCCTGTTCGGAGAAATTCACGCCAAGGCGTTTTTCAAAGAAAAATTTCACCGGATTTTTCACAAACGCCACCAAGCGGGAAAACTCGATTTCTTCTTCCGGCTCCGCATCCGCCATCATCg                                                                                                                                                                                                                 >  1:721882/1‑251 (MQ=255)                                    gACTTTCAGACGCGCAAAAAACGCGTCGGTTTGCTGCTCGTCTGCTTCTAACAAATCCGCATGAATGGCGTATTTTTCCAAGGCATTATCCAAGGCGAAATTTTCGCTATCGGCAATGCTTTCTTCCTGTTCGGAGAAATTCACGCCAAGGCGTTTTTCAAAGAAAAATTTCACCGGATTTTTCACAAACGCCACCAAGCGGGAAAACTCGAtt                                                                                                                                                                                                                                                <  1:328339/214‑1 (MQ=255)                                     aCTTTCAGACGCGCAAAAAACGCGTCGGTTTGCTGCTCGTCTGCTTCTAACAAATCCGCATGAATGGCGTATTTTTCCAAGGCATTATCCAAGGCGAAATTTTCGCTATCGGCAATGCTTTCTTCCTGTTCGGAGAAATTCACGCCAAGGCGTTTTTCAAAGAAAAATTTCACCGGATTTTTCACAAACGCCACCAAGCGGGAAAACTCGATTTCTTCTTCCGGCTCCGCATCCGCCATCATCGGCTGAAt                                                                                                                                                                                                          >  1:800121/1‑251 (MQ=255)                                             aCGCGCAAAAAACGCGTCGGTTTGCTGCTCGTCTGCTTCTAACAAATCCGCATGAATGGCGTATTTTTCCAAGGCATTATCCAAGGCGAAATTTTCGCTATCGGCAATGCTTTCTTCCTGTTCGGAGAAATTCACGCCAAGGCGTTTTTCAAAGAAAAATTTCACCGGATTTTTCACAAACGCCACCAAGCGGGAAAACTCGATTTCTTCTTCCGGCTCCGCATCCGCCATCATCGGCTGAATAAAAt                                                                                                                                                                                                     <  1:889286/248‑1 (MQ=255)                                             aCGCGCAAAAAACGCGTCGGTTTGCTGCTCGTCTGCTTCTAACAAATCCGCATGAATGGCGTATTTTTCCAAGGCATTATCCAAGGCGAAATGTTCGCTATCGGCAATGCTTTCTTCCTGTTCGGAGAAATTAACGCCAAGGCGTTTTTCAAAGAAAAATTTCACCGGATTTTTCACAAACGCCACCAAGCGGGAAAACTCGATTTCTTCTTCCGGCTCCGCATCCGCCATCATCGGCTGAATAAAAt                                                                                                                                                                                                     <  1:86227/248‑1 (MQ=255)                                                   aaaaaaCGCGTCGGTTTGCTGCTCGTCTGCTTCTAACAAATCCGCATGAATGGCGTATTTTTCCAAGGCATTATCCAAGGCGAAATTTTCGCTATCGGCAATGCTTTCTTCCTGTTCGGAGAAATTCACGCCAAGGCGTTTTTCAAAGAAAAATTTCACCGGATTTTTCACAAACGCCACCAAGCGGGAAAACTCGATTTCTTCTTCCGGCTCCGCATCCGCCATCATCGGCTGAATAAAATCCTGCAAgg                                                                                                                                                                                            <  1:420152/251‑1 (MQ=255)                                                     aaaaCGCGTCGGTTTGCTGCTCGTCTGCTTCTAACAAATCCGCATGAATGGCGTATTTTTCCAAGGCATTATCCAAGGCGAAATTTTCGCTATCGGCAATGCTTTCTTCCTGTTCGGAGAAATTCACGCCAAGGCGTTTTTCAAAGAAAAATTTCACCGGATTTTTCACAAACGCCACCAAGCGGGAAAACTCGATTTCTTCTTCCGGCTCCGCATCCGCCATCATCGGCTGAATAAAATCCTGCAAGGtt                                                                                                                                                                                          <  1:123813/251‑1 (MQ=255)                                                               ggTTTGCTGCTCGTCTGCTTCTAACAAATCCGCATGAATGGCGTATTTTTCCAAGGCATTATCCAAGGCGAAATTTTCGCTATCGGCAATGCTTTCTTCCTGTTCGGAGAAATTCACGCCAAGGCGTTTTTCAAAGAAAAATTTCACCGGATTTTTCACAAACGCCACCAAGCGGGAAAACTCGATTTcttcttc                                                                                                                                                                                                                                        <  1:601988/195‑7 (MQ=255)                                                               ggTTTGCTGCTCGTCTGCTTCTAACAAATCCGCATGAATGGCGTATTTTTCCAAGGCATTATCCAAGGCGAAATTTTCGCTATCGGCAATGCTTTCTTCCTGTTCGGAGAAATTCACGCCAAGGCGTTTTTCAAAGAAAAATTTCACCGGATTTTTCACAAACGCCACCAAGCGGGAAAACTCGATTTCTTCTTCCGGCTCCGCATCCGCCATCATCGGCTGAATaa                                                                                                                                                                                                        >  1:638378/1‑227 (MQ=255)                                                                     ctgctCGTCTGCTTCTAACAAATCCGCATGAATGGCGTATTTTTCCAAGGCATTATCCAAGGCGAAATTTTCGCTATCGGCAATGCTTTCTTCCTGTTCGGAGAAATTCACGCCAAGGCGTTTTTCAAAGAAAAATTTCACCGGATTTTTCACAAACGCCACCAAGCGGGAAAACTCGATTTCTTCTTCCGGCTCCGCATCCGCCATCATCGGCTGAATAAAATCCTGCAAGGTTTGATTTGCCTGCGAAt                                                                                                                                                                          <  1:717573/251‑1 (MQ=255)                                                                               gcttctaacaAATCCGCATGAATGGCGTATTTTTCCAAGGCATTATCCAAGGCGAAATTTTCGCTATCGGCAATGCTTTCTTCCTGTTCGGAGAAATTCACGCCAAGGCGTTTTTCAAAGAAAAATTTCACCGGATTTTTCACAAACGCCACCAAGCGGGAAAACTCGATTTCTTCTTCCGGCTCCGCATCCGCCATCATCGGCTGAATAAAATcc                                                                                                                                                                                                   <  1:117100/208‑1 (MQ=255)                                                                               gcttctaacaAATCCGCATGAATGGCGTATTTTTCCAAGGCATTATCCAAGGCGAAATTTTCGCTATCGGCAATGCTTTCTTCCTGTTCGGAGAAATTCACGCCAAGGCGTTTTTCAAAGAAAAATTTCACCGGATTTTTCACAAACGCCACCAAGCGGGAAAACTCGATTTCTTCTTCCGGCTCCGCATCCGCCATCATCGGCTGAATAAAATCCTGCAAGGTTTGATTTGCCTGCGAATTTGCCAAAgg                                                                                                                                                                >  1:61372/9‑251 (MQ=255)                                                                                cttctaacaAATCCGCATGAATGGCGTATTTTTCCAAGGCATTATCCAAGGCGAAATTTTCGCTATCGGCAATGCTTTCTTCCTGTTCGGAGAAATTCACGCCAAGGCGTTTTTCAAAGAAAAATTTCACCGGATTTTTCACAAACGCCACCAAGCGGGAAAACTCGATTTCTTCTTCCGGCTCCGCATCCGCCATCATCGGCTGAATAAAATCCTGCAAGGTTTGATTTGCCTGCGAATTTGCCAAAgg                                                                                                                                                                >  1:296274/8‑250 (MQ=255)                                                                                cttctaacaAATCCGCATGAATGGCGTATTTTTCCAAGGCATTATCCAAGGCGAAATTTTCGCTATCGGCAATGCTTTCTTCCTGTTCGGAGAAATTCACGCCAAGGCGTTTTTCAAAGAAAAATTTCACCGGATTTTTCACAAACGCCACCAAGCGGGAAAACTCGATTTCTTCTTCCGGCTCCGCATCCGCCATCATCGGCTGAATAAAATCCTGCAAGGTTTGATTTGCCTGCGAATTTGCCAAAgg                                                                                                                                                                <  1:852350/243‑1 (MQ=255)                                                                                cttctaacaAATCCGCATGAATGGCGTATTTTTCCAAGGCATTATCCAAGGCGAAATTTTCGCTATCGGCAATGCTTTCTTCCTGTTCGGAGAAATTCACGCCAAGGCGTTTTTCAAAGAAAAATTTCACCGGATTTTTCACAAACGCCACCAAGCGGGAAAACTCGATTTCTTCTTCCGGCTCCGCATCCGCCATCATCGGCTGAATAAAATCCTGCAAGGTTTGATTTGCCTGCGAATTTGCCAAAgg                                                                                                                                                                <  1:210145/243‑1 (MQ=255)                                                                                    taacaAATCCGCATGAATGGCGTATTTTTCCAAGGCATTATCCAAGGCGAAATTTTCGCTATCGGCAATGCTTTCTTCCTGTTCGGAGAAATTCACGCCAAGGCGTTTTTCAAAGAAAAATTTCACCGGATTTTTCACAAACGCCACCAAGCGGGAAAACTCGATTTCTTCTTCCGGCTCCGCATCCGCCATCATCGGCTGAATAAAATCCTGCAAGGTTTGATTTGCCTGCGAAt                                                                                                                                                                          <  1:588551/233‑1 (MQ=255)                                                                                        aaaTCCGCATGAATGGTGTATTTTTCCAAGGCATTATCCAAGGCGAAATTTTCGCTATCGGCAATGCTTTCTTCCTGTTCGGAGAAATTCACGCCAAGGCGTTTTTCAAAGAAAAATTTCACCGGATTTTTCACAAACGCCACCAAGCGGGAAAACTCGATTTCTTCTTCCGGCTCCGCATCCGCCATCATCGGCTGAATAAAATCCTGCAAGGTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCCACTg                                                                                                                                                       <  1:525848/251‑1 (MQ=255)                                                                                         aaTCCGCATGAATGGCGTATTTTTCCAAGGCATTATCCAAGGCGAAATTTTCGCTATCGGCAATGCTTTCTTCCTGTTCGGAGAAATTCACGCCAAGGCGTTTTTCAAAGAAAAATTTCACCGGATTTTTCACAAACGCCACCAAGCGGGAAAACTCGATTTCTTCTTCCGGCTCCGCATCCGCCATCATCGGCTGAATAAAATCCTGCaa                                                                                                                                                                                              >  1:52211/1‑211 (MQ=255)                                                                                         aaTCCGCATGAATGGCGTATTTTTCCAAGGCATTATCCAAGGCGAAATTTTCGCTATCGGCAATGCTTTCTTCCTGTTCGGAGAAATTCACGCCAAGGCGTTTTTCAAAGAAAAATTTCACCGGATTTTTCACAAACGCCACCAAGCGGGAAAACTCGATTTCTTCTTCCGGCTCCGCATCCGCCATCATCGGCTGAATAAAATCCTGCAAGGTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCTACTGt                                                                                                                                                      <  1:258587/251‑1 (MQ=255)                                                                                         aaTCCGCATGAATGGCGTATTTTTCCAAGGCATTATCCAAGGCGAAATTTTCGCTATCGGCAATGCTTTCTTCCTGTTCGGAGAAATTCACGCCAAGGCGTTTTTCAAAGAAAAATTTCACCGGATTTTTCACAAACGCCACCAAGCGGGAAAACTCGATTTCTTCTTCCGGCTCCGCATCCGCCATCATCGGCTGAATAAAATCCTGCAAGGTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCCACTGt                                                                                                                                                      <  1:342022/251‑1 (MQ=255)                                                                                             cGCATGAATGGCGTATTTTTCCAAGGCATTATCCAAGGCGAAATTTTCGCTATCGGCAATGCTTTCTTCCTGTTCGGAGAAATTCACGCCAAGGCGTTTTTCAAAGAAAAATTTCACCGGATTTTTCACAAACGCCACCAAGCGGGAAAACTCGATTTCTTCTTCCGGCTCCGCATCCGCCATCATCGGCTGAATAAAATCCTGCAAGGTTTGATTTGCCTGCGAATTTGCCAAAggc                                                                                                                                                               >  1:798453/1‑237 (MQ=255)                                                                                                   tgacaagtgtttttCCAAGGCATTATCCAAGGCGAAATTTTCGCTATCGGCAATGCTTTCTTCCTGTTCGGAGAAATTCACGCCAAGGCGTTTTTCAAAGAAAAATTTCACCGGATTTTTCACAAACGCCACCAAGCGGGAAAACTCGATTTCTTCTTCCGGCTCCGCATCCGCCATCATCGGCTGAATAAAATCCTGCAAGGTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAg                                                                                                                                            <  1:829681/242‑1 (MQ=255)                                                                                                   aaTGGCGTATTTTTCCAAGGCATTATCCAAGGCGAAATTTTCGCTATCGGCAATGCTTTCTTCCTGTTCGGAGAAATTCACGCCAAGGCGTTTTTCAAAGAAAAATTTCACCGGATTTTTCACAAACGCCACCAAGCGGGAAAACTCGATTTCTTCTTCCGGCTCCGCATCCGCCATCATCGGCTGAATAAAATCCTGCAAGGTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAg                                                                                                                                            <  1:191840/251‑1 (MQ=255)                                                                                                   aaTGGCGTATTTTTCCAAGGCATTATCCAAGGCGAAATTTTCGCTATCGGCAATGCTTTCTTCCTGTTCGGAGAAATTCACGCCAAGGCGTTTTTCAAAGAAAAATTTCACCGGATTTTTCACAAACGCCACCAAGCGGGAAAACTCGATTTCTTCTTCCGGCTCCGCATCCGCCATCATCGGCTGAATAAAATCCTGCAAGGTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAg                                                                                                                                            <  1:431620/251‑1 (MQ=255)                                                                                                     tGGCGTATTTTTCCAAGGCATTATCCAAGGCGAAATTTTCGCTATCGGCAATGCTTTCTTCCTGTTCGGAGAAATTCACGCTAAGGCGTTTTTCAAAGAAAAATTTCACCGGATTTTTCACAAACGCCACCAAGCGGGAAAACTCGATTTCTTCTTCCGGCTCCGCATCCGCCATCATCGGCTGAATAAAATCCTGCAAGGTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAGAt                                                                                                                                          <  1:440580/251‑1 (MQ=255)                                                                                                     tGGCGTATTTTTCCAAGGCATTATCCAAGGCGAAATTTTCGCTATCGGCAATGCTTTCTTCCTGTTCGGAGAAATTCACGCCAAGGCGTTTTTCAAAGAAAAATTTCACCGGATTTTTCACAAACGCCACCAAGCGGGAAAACTCGATTTCTTCTTCCGGCTCCGCATCCGCCATCATCGGCTGAATAAAATCCTGCAAGGTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAGAt                                                                                                                                          <  1:623965/251‑1 (MQ=255)                                                                                                     tGGCGTATTTTTCCAAGGCATTATCCAAGGCGAAATTTTCGCTATCGGCAATGCTTTCTTCCTGTTCGGAGAAATTCACGCCAAGGCGTTTTTCAAAGAAAAATTTCACCGGATTTTTCACAAACGCCACCAAGCGGGAAAACTCGATTTCTTCTTCCGGCTCCGCATCCGCCATCATCGGCTGAATAAAATCCTGCAAGGTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAGAt                                                                                                                                          <  1:521263/251‑1 (MQ=255)                                                                                                         gTATTTTTCCAAGGCATTATCCAAGGCGAAATTTTCGCTATCGGCAATGCTTTCTTCCTGTTCGGAGAAATTCACGCCAAGGCGTTTTTCAAAGAAAAATTTCACCGGATTTTTCACAAACGCCACCAAGCGGGAAAACTCGATTTCTTCTTCCGGCTCCGCATCCGCCCTCATCGGCTGAATAAAATCCTGCAAGGTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAGATTGCa                                                                                                                                      >  1:455530/1‑251 (MQ=255)                                                                                                         gTATTTTTCCAAGGCATTATCCAAGGCGAAATTTTCGCTATCGGCAATGCTTTCTTCCTGTTCGGAGAAATTCACGCCAAGGCGTTTTTCAAAGAAAAATTTCACCGGATTTTTCACAAACGCCACCAAGCGGGAAAACTCGATTTCTTCTTCCGGCTCCGCATCCGCCATCATCGGCTGAATAAAAt                                                                                                                                                                                                     >  1:229831/1‑188 (MQ=255)                                                                                                         gTATTTTTCCAAGGCATTATCCAAGGCGAAATTTTCGCTATCGGCAATGCTTTCTTCCTGTTCGGAGAAATTCACGCCAAGGCGTTTTTCAAAGAAAAATTTCACCGGATTTTTCACAAACGCCACCAAGCGGGAAAACTCGATTTCTTCTTCCGGCTCCGCATCCGCCATCATCGGCTGAATAAAATCTTGCAAGTTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAGATTg                                                                                                                                        >  1:867636/1‑249 (MQ=255)                                                                                                          tATTTTTCCAAGGCATTATCCAAGGCGAAATTTTCGCTATCGGCAATGCTTTCTTCCTGTTCGGAGAAATTCACGCCAAGGCGTTTTTCAAAGAAAAATTTCACCGGATTTTTCACAAACGCCACCAAGCGGGAAAACTCGATTTCTTCTTCCGGCTCCGCATCCGCCATCATCGGCTGAATAAAATCCTGCAAGGTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAGATTGCAt                                                                                                                                     <  1:687923/251‑1 (MQ=255)                                                                                                               ttCCAAGGCATTATCCAAGGCGAAATTTTCGCTATCGGCAATGCTTTCTTCCTGTTCGGAGAAATTCACGCCAAGGCGTTTTTCAAAGAAAAATTTCACCGGATTTTTCACAAACGCCACCAAGCGGGAAAACTCGATTTCTTCTTCCGGGTCAGCATCCGCCATCATCGGCTGAATAAAATCCTGCAAGGTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAGATTGCATAAAAg                                                                                                                                <  1:383112/251‑1 (MQ=255)                                                                                                               ttCCAAGGCATTATCCAAGGCGAAATTTTCGCTATCGGCAATGCTTTCTTCCTGTTCGGAGAAATTCACGCCAAGGCGTTTTTCAAAGAAAAATTTCACCGGATTTTTCACAAACGCCACCAAGCGGGAAAACTCGATTTCTTCTTCCGGCTCCGCATCCGCCATCATCGGCTGAATAAAATCCTGCAAGGTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAGATTGCATAAAAg                                                                                                                                <  1:853074/251‑1 (MQ=255)                                                                                                               ttCCAAGGCATTATCCAAGGCGAAATTTTCGCTATCGGCAATGCTTTCTTCCTGTTCGGAGAAATTCACGCCAAGGCGTTTTTCAAAGAAAAATTTCACCGGATTTTTCACAAACGCCACCAAGCGGGAAAACTCGATTTCTTCTTCCGGCTCCGCATCCGCCATCATCGGCTGAATAAAATCCTGCAAGGTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAGATTGCATAAAAg                                                                                                                                >  1:780869/1‑251 (MQ=255)                                                                                                               ttCCAAGGCATTATCCAAGGCGAAATTTTCGCTATCGGCAATGCTTTCTTCCTGTTCGGAGAAATTCACGCCAAGGCGTTTTTC‑AAGAAAAATTTCACCGGATTTTTCACAAACGCCACCAAGCGGGAAAACTCGATTTCTTCTTCCGGCTCCGCATCCGCCATCATCGGCTGAATAAAATCCTGCAAGGTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAGATTGCATAAAAGt                                                                                                                               >  1:545110/1‑251 (MQ=255)                                                                                                                      gCATTATCCAAGGCGAAATTTTCGCTATCGGCAATGCTTTCTTCCTGTTCGGAGAAATTCACGCCAAGGCGTTTTTCAAAGAAAAATTTCACCGGATTTTTCACAAACGCCACCAAGCGGGAAAACTCGATTTCTTCTTCCGGCTCCGCATCCGCCATCATCGGCTGAATaa                                                                                                                                                                                                        >  1:213294/1‑172 (MQ=255)                                                                                                                      gCATTATCCAAGGCGAAATTTTCGCTATCGGCAATGCTTTCTTCCTGTTCGGAGAAATTCACGCCAAGGCGTTTTTCAAAGAAAAATTTCACCGGATTTTTCACAAACGCCACCAAGCGGGAAAACTCGATTTCTTCTTCCGGCTCCGCATCCGCCATCATCGGCTGAATAAAATCCTGCAAGGTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAGATTGCATAAAAGTGcggt                                                                                                                          >  1:396076/1‑247 (MQ=255)                                                                                                                      gCATTATCCAAGGCGAAATTTTCGCTATCGGCAATGCTTTCTTCCTGTTCGGAGAAATTCACGCCAAGGCGTTTTTCAAAGAAAAATTTCACCGGATTTTTCACAAACGCCACCAAGCGGGAAAACTCGATTTCTTCTTCCGGCTCCGCATCCGCCATCATCGGCTGAATAAAATCCTGCAAGGTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAGATTGCATAAAAGTGcggt                                                                                                                          >  1:868613/1‑247 (MQ=255)                                                                                                                                                    gCAATGCTTTCTTCCTGTTCGGAGAAATTCACGCCAAGGCGTTTTTCAAAGAAAAATTTCACCGGATTTTTCACAAACGCCACCAAGCGGGAAAACTCGATTTCTTCTTCCGGCTCCGCATCCGCCATCATCGGCTGAATAAAAt                                                                                                                                                                                                     >  1:815274/1‑145 (MQ=255)                                                                                                                                                     cAATGCTTTCTTCCTGTTCGGAGAAATTCACGCCAAGGCGTTTTTCAAAGAAAAATTTCACCGGATTTTTCACAAACGCCACCAAGCGGGAAAACTCGATTTCTTCTTCCGGCTCCGCATCCGCCATCATCGGCTGAATAAAATCCTGCAAGGTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAGATTGCATAAAAGTGCGGTCGTTTTTTTCAAAGTTTTTGCGACTGAacgc                                                                                           <  1:561396/250‑3 (MQ=255)                                                                                                                                                       aTGCTTTCTTCCTGTTCGGAGAAATTCACGCCAAGGCGTTTTTCAAAGAAAAATTTCACCGGATTTTTCACAAACGCCACCAAGCGGGAAAACTCGATTTCTTCTTCCGGCTCCGCATCCGCCATCATCGGCTGAATAAAATCCTGCAAGGTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAGATTGCATAAAAGTGCGGTCGTTTTTTTCAAAGTTTTTGCGACTGAacgccgt                                                                                        <  1:4525/251‑6 (MQ=255)                                                                                                                                                         gCTTTCTTCCTGTTCGGAGAAATTCACGCCAAGGCGTTTTTCAAAGAAAAATTTCACCGGATTTTTCACAAACGCCACCAAGCGGGAAAACTCGATTTCTTCTTCCGGCTCCGCATCCGCCATCATCGGCTGAATAAAATCCTGCAAGGTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAGATTGCATAAAAg                                                                                                                                >  1:94837/1‑209 (MQ=255)                                                                                                                                                         gCTTTCTTCCTGTTCGGAGAAATTCACGCCAAGGCGTTTTTCAAAGAAAAATTTCACCGGATTTTTCACAAACGCCACCAAGCGGGAAAACTCGATTTCTTCTTCCGGCTCCGCATCCGCCATCATCGGCTGAATAAAATCCTGCAAGGTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAGATTGCATAAAAGTGCGGTCGTTTTTTTCAAAGTTTTTGCGAc                                                                                                  <  1:447899/239‑1 (MQ=255)                                                                                                                                                         gCTTTCTTCCTGTTCGGAGAAATTCACGCCAAGGCGTTTTTCAAAGAAAAATTTCACCGGATTTTTCACAAACGCCACCAAGCGGGAAAACTCGATTTCTTCTTCCGGCTCCGCATCCGCCATCATCGGCTGAATAAAATCCTGCAAGGTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAGATTGCATAAAAGTGCGGTCGTTTTTTTCAAAGTTTTTGCGACTGAacgccgtca                                                                                      >  1:399884/1‑244 (MQ=255)                                                                                                                                                         gCTTTCTTCCTGTTCGGAGAAATTCACGCCAAGGCGTTTTTCAAAGAAAAATTTCACCGGATTTTTCACAAACGCCACCAAGCGGGAAAACTCGATTTCTTCTTCCGGCTCCGCATCCGCCATCATCGGCTGAATAAAATCCTGCAAGGTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAGATTGCATAAAAGTGCGGTCGTTTTTTTCAAAGTTTTTGCGACTGAacgccgtca                                                                                      >  1:242350/1‑244 (MQ=255)                                                                                                                                                         gCTTTCTTCCTGTTCGGAGAAATTCACGCCAAGGCGTTTTTCAAAGAAAAATTTCACCGGATTTTTCACAAACGCCACCAAGCGGGAAAACTCGATTTCTTCTTCCGGCTCCGCATCCGCCATCATCGGCTGAATAAAATCCTGCAAGGTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAGATTGCATAAAAGTGCGGTCGTTTTTTTCAAAGTTTTTGCGACTGAacgccgtca                                                                                      >  1:805949/1‑244 (MQ=255)                                                                                                                                                         gCTTTCTTCCTGTTCGGAGAAATTCACGCCAAGGCGTCTTTCAAAGAAAAATTTCACCGGATTTTTCACAAACGCCACCAAGCGGGAAAACTCGATTTCTTCTTCCGGCTCCGCATCCGCCATCATCGGCTGAATAAAATCCTGCAAGGTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAGATTGCATAAAAGTGCGGTCGTTTTTTTCAAAGCTTTTGCGACTGAacgccgtca                                                                                      >  1:881413/1‑244 (MQ=255)                                                                                                                                                          cTTTCTTCCTGTTCGGAGAAATTCACGCCAAGGCGTTTTTCAAAGAAAAATTTCACCGGATTTTTCACAAACGCCACCAAGCGGGAAAACTCGATTTCTTCTTCCGGCTCCGCATCCGCCATCATCGGCTGAATAAAATCCTGCAAGGTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAGATTGCATAAAAGTGCGGTCGTTTTTTTCAAAGTTTTTGCGACTGAacgccgtca                                                                                      >  1:122550/1‑243 (MQ=255)                                                                                                                                                            ttcttcCTGTTCGGAGAAATTCACGCCAAGGCGTTTTTCAAAGAAAAATTTCACCGGATTTTTCACAAACGCCACCAAGCGGGAAAACTCGATTTCTTCTTCCGGCTCCGCATc                                                                                                                                                                                                                            <  1:170342/114‑1 (MQ=255)                                                                                                                                                                 ccTGTTCGGAGAAATTCACGCCAAGGCGTTTTTCAAAGAAAAATTTCACCGGATTTTTCACAAACGCCACCAAGCGGGAAAACTCGATTTCTTCTTCCGGCTCCGCATCCGCCATCATCGGCTGAATAAAATCCTGCAAGGTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAGATTGCATAAAAGTGCGGTCGTTTTTTTCAAAGTTTTTGCGACTGAACGCCGTCATGCCAtgt                                                                              <  1:305001/251‑1 (MQ=255)                                                                                                                                                                             aaTTCACGCCAAGGCGTTTTTCAAAGAAAAATTTCACCGGATTTTTCACAAACGCCACCAAGCGGGAAAACTCGATTTCTTCTTCCGGCTCCGCATCCGCCATCATCGGCTGAATAAAATCCTGCAAGGTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAGATTGCATAAAAGTGCGGTCGTTTTTTTCAAAGTTTTTGCGACTGAACGCCGTCATGCCATGTTGTTGAATCAGt                                                                  >  1:219212/1‑251 (MQ=255)                                                                                                                                                                              aTTCACGCCAAGGCGTTTTTCAAAGAAAAATTTCACCGGATTTTTCACAAACGCCACCAAGCGGGAAAACTCGATTTCTTCTTCCGGCTCCGCATCCGCCATCATCGGCTGAATAAAATCCTGCAAGGTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCCAc                                                                                                                                                         >  1:771495/1‑163 (MQ=255)                                                                                                                                                                               ttCACGCCAAGGCGTTTTTCAAAGAAAAATTTCACCGGATTTTTCACAAACGCCACCAAGCGGGAAAACTCGATTTCTTCTTCCGGCTCCGCATCCGCCATCATCGGCTGAATAAAATCCTGCAAGGTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAGATTGCATAAAAGTGCGGTCGTTTTTTTCAAAGTTTTTGCGACTGAACGCCGTCATGCCATGTTGTTGAATCAGTCg                                                                <  1:585432/251‑1 (MQ=255)                                                                                                                                                                                       aaGGCGTTTTTCAAAGAAAAATTTCACCGGATTTTTCACAAACGCCACCAAGCGGGAAAACTCGATTTCTTCTTCCGGCTCCGCATCCGCCATCATCGGCTGAATAAAATCCTGCAAGGTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAGATTGCATAAAAGTGCGGTCGTTTTTTTCAAAGTTTTTGCGACTGAACGCCGTCATGCCATGTTGTTGAATCAGTCGTTCGCGcc                                                        <  1:292541/251‑1 (MQ=255)                                                                                                                                                                                         ggCGTTTTTCAAAGAAAAATTTCACCGGATTTTTCACAAACGCCACCAAGCGGGAAAACTCGATTTCTTCTTCCGGCTCCGCATCCGCCATCATCGGCTGAATAAAATCCTGCAAGGTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAGATTGCATAAAAGTGCGGTCGTTTTTTTCAAAGTTTTTGCGACTGAACGCCGTCATGCCATGTTGTTGAATCAGTCGTTCGCGCCa                                                       >  1:104576/1‑250 (MQ=255)                                                                                                                                                                                                       aaaaaTTTCACCGGATTTTTCACAAACGCCACCAAGCGGGAAAACTCGATTTCTTCTTCCGGCTCCGCATCCGCCATCATCGGCTGAATAAAATCCTGCAAGGTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAGATTGCATAAAAGTGCGGTCGTTTTTTTCAAAGTTTTTGCGACTGAACGCCGTCATGCCATGTTGTTGAATCAGTCGTTCGCGCCACTTGAGATTTCCCg                                         <  1:215289/250‑1 (MQ=255)                                                                                                                                                                                                         aaaTTTCACCGGATTTTTCACAAACGCCACCAAGCGGGAAAACTCGATTTCTTCTTCCGGCTCCGCATCCGCCATCATCGGCTGAATAAAATCCTGCAAGGTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAGATTGCATAAAAGTGCGGTCGTTTTTTTCAAAGTTTTTGCGACTGAACGCCGTCATGCCATGTTGTTGAATCAGTCGTTCGCGCCACTTGAGATTTCCCg                                         >  1:331458/1‑248 (MQ=255)                                                                                                                                                                                                                    gATTTTTCACAAACGCCACCAAGCGGGAAAACTCGATTTCTTCTTCCGGCTCCGCATCCGCCATCATCGGCTGAATAAAATCCTGCAAGGTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAGATTGCATAAAAGTGCGGTCGTTTTTTTCAAAGTTTTTGCGACTGAACGCCGTCATGCCATGttgttg                                                                         >  1:346375/1‑205 (MQ=255)                                                                                                                                                                                                                             caAACGCCACCAAGCGGGAAAACTCGATTTCTTCTTCCGGCTCCGCATCCGCCATCATCGGCTGAATAAAATCCTGCAAGGTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAGATTGCATAAAAGTGCGGTCGTTTTTTTCAAAGTTTTTGCGACTGAACGCCGTCATGCCATGTTGTTGAATCAGTCGTTCGCGCCACTTGAGATTTCCCGCTGGGGTATTTTCTGCCGGCAGa                  <  1:801513/251‑1 (MQ=255)                                                                                                                                                                                                                               aaCGCCACCAAGCGGGAAAACTCGATTTCTTCTTCCGGCTCCGCATCCGCCATCATCGGCTGAATAAAATCCTGCAAGGTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAGATTGCATAAAAg                                                                                                                                >  1:436528/1‑139 (MQ=255)                                                                                                                                                                                                                                   ccaccaAGCGGGAAAACTCGATTTCTTCTTCCGGCTCCGCATCCGCCATCATCGGCTGAATAAAATCCTGCAAGGTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAGATTGCATAAAAGTGCGGTCGTTTTTTTCAAAGTTTTTGCGACTGAACGCCGTCATGCCATGTTGTTGAATCAGTCGTTCGCGCCACTTGAGATTTccc                                          >  1:629599/1‑221 (MQ=255)                                                                                                                                                                                                                                        aaGCGGGAAAACTCGATTTCTTCTTCCGGCTCCGCATCCGCCATCATCGGCTGAATAAAATCCTGCAAGGTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAGATTGCATAAAAGTGCGGTCGTTTTTTTCAAAGTTTTTGCGACTGAACGCCGTCATGCCATGTTGTTGAATCAGTCGTTCGCGCCACTTGAGATTTCCCGCTGGGGTATTTTCTGCCGGCAGATTTTCCACGAt       >  1:494918/1‑251 (MQ=255)                                                                                                                                                                                                                                          gCGGGAAAACTCGATTTCTTCTTCCGGCTCCGCATCCGCCATCATCGGCTGAATAAAATCCTGCAAGGTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAGATTGCATAAAAg                                                                                                                                <  1:118026/128‑1 (MQ=255)                                                                                                                                                                                                                                             ggAAAACTCGATTTCTTCTTCCGGCTCCGCATCCGCCATCATCGGCTGAATAAAATCCTGCAAGGTTTGATTTGCCTGCGAATTTGCCAAAGGCAGCCACTGTTGCGCGAAAGATTGCATAAAAGTGCGGTCGTTTTTTTCAAAGTTTTTGCGTCTGAACGCCGTCATGCCATGTTGTTGAATCAGTCGTTCGCGCCACTTGAGATTTCCCGCTGGGGTATTTTCTGCCGGCAGATTTTCCACGATGTAAt  >  1:691851/1‑251 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                  ATGGCGGAGAACTCCCCGCGCGGCAACACGCCTTTGACTTTCAGACGCGCAAAAAACGCGTCGGTTTGCTGCTCGTCCGCTTCCACCAAATCCGCATGAATGGCGTATTTTTCCAAGGCATTATCCAAGGCGAAATTTTCGCTGTCGGCAATGCTTTCTTCCTGTTCGGAGAAATTCACGCCAAGGCGTTTTTCAAAGAAAAATTTCACCGGATTTTTCACAAACGCCACCAAGCGGGAAAACTCAATTTCATTTTCCGGCTCCGCATCCGTCATCATCGGCTGAATAAAATCCTGCAAGGTTTGATTTACCTGCGAATTTGCCAAAGGTAGCCACTGTTGCGCGAAAGATTGCATAAAAGTGCAGTCGTTTTTTTCAAAGTTTTTACGACTGAACACGGTCATGCCATGTTGTTGAATCAGTCGTTCGCGCCACTTGAGATTTCCCGATGGGGTATTTTCCGCCGGCAGATTTTCCACGATGTAAT  >  1/1830958‑1831444

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑