Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,830,921	G→A	intergenic (+49021/+4463)	unknown → / ← unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,830,921	0	G	A	100.0%	162.6 / NA	57	intergenic (+49021/+4463)	unknown/unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (37/20);  total (37/20)

CTGTTCCTGCGTGGCGCACAACAATAAGTGATAAAGCCAAGCTTCAATACGATCTTTATCTTTTACCGTCGCCATGCGCCAAGTGACGCGCTGTAGATTGTCACCGTATAAATGGGAAATGTTGCCCGACAACGTCATGTCACCCTCTTGGGTCGGCACGGTGAATTGCACAAAACGGCTTTGCGGCGTTTGGCTTACATAATCGGCGATCTTGCCTTTGAATTCCGCCACATCCGCCAGCAACTGATTGGCATAAATGGCGGAGAACTCCCCGCGCGGCAACACGCCTTTGACTTTCAGACGCGCAAAAAACGCGTCGGTTTGCTGCTCGTCCGCTTCCACCAAATCCGCATGAATGGCGTATTTTTCCAAGGCATTATCCAAGGCGAAATTTTCGCTGTCGGCAATGCTTTCTTCCTGTTCGGAGAAATTCACGCCAAGGCGTTTTTCAAAGAA  >  1/1830702‑1831157                                                                                                                                                                                                                            |                                                                                                                                                                                                                                             cTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATACGATCTTTGTCTTTTACCGTCGCCATGCGCCAAGTGACGCGCTGTAGATTGTCACCGTATAAATAGGATATGTTGCCCGACAACGTCATGTCACCCTCTTGGGTCAGCACGGTGAATTGCAAAAAACGGCTTTGCGGCGTTTGGCTTACATAATCGGCGATATTGCCTTTAAATTCCGCCACATCCGCCAGCAACTGATTgg                                                                                                                                                                                                               >  1:891271/1‑251 (MQ=255)                  ataacaaTAAGTGATAAAGCCAGGCTTCAATACGATCTTTGTCTTTTACCGTCGCCATGCGCCAAGTGACGCGCTGTAGATTGTCACCGTATAAATAGGATATGTTGCCCGACAACGTCATGTCACCCTCTTGGGTCAGCACGGTGAATTGCAAAAAACGGCTTTGCGGCGTTTGGCTTACATAATCGGCGATATTGCCTTTAAATTCCGCCACATCCGCCAGCAACTGATTGGCATAAATGGCGGAGAAc                                                                                                                                                                                              <  1:270001/249‑1 (MQ=255)                            gTGATAAAGCCAGGCTTCAATACGATCTTTGTCTTTTACCGTCGCCATGCGCCAAGTGACGCGCTGTAGATTGTCACCGTATAAATAGGATATGTCGCCCGACAACGTCATGTCACCCTCTTGGGTCAGCACGGTGAATTGCAAAAAACGGCTTTGCGGCGTTTGGCTTACATAATCGGCGATATTGCCTTTAAATTCCGCCACATCCGCCAGCAACTGATTGGCATAAATGGCGGAGAACTCCCcgcgcg                                                                                                                                                                                    >  1:223632/1‑251 (MQ=255)                             tGATAAAGCCAGGCTTCAATACGATCTTTGTCTTTTACCGTCGCCATGCGCCAAGTGACGCGCTGTAGATTGTCACCGTATAAATAGGATATGTTGCCCGACAACGTCATGTCACCCTCTTGGGTCAGCACGGTGAATTGCAAAAAACGGCTTTGCGGCGTTTGGCTTACATAATCGGCGATATTGCCTTTAAATTCCGCCACATCCGCCAGCAACTGATTGGCATAAATGGCGGAGAACTCCCcgcgc                                                                                                                                                                                     <  1:17493/249‑1 (MQ=255)                                   aGCCAGGCTTCAATACGATCTTTGTCTTTTACCGTCGCCATGCGCCAAGTGACGCGCTGTAGATTGTCACCGTATAAATAGGATATGTTGCCCGACAACGTCATGTCACCCTCTTGGGTCAGCACGGTGAATTGCAAAAAACGGCTTTGCGGCGTTTGGCTTACATAATCGGCGATATTGCCTTTAAATTCCGCCACATCCGCCAGCAACTGATTGGCATAAATGGCGGAGAACTCCCCGCGCGGCAaca                                                                                                                                                                              >  1:893814/1‑250 (MQ=255)                                          cTTCAATACGATCTTTGTCTTTTACCGTCGCCATGCGCCAAGTGACGCGCTGTAGATTGTCACCGTATAAATAGGATATGTTGCCCGACAACGTCATGTCACCCTCTTGGGTCAGCACGGTGAATTGCAAAAAACGGCTTTGCGGCGTTTGGCTTACATAATCGGCGATATTGCCTTTAAATTCCGCCACATCCGCCAGCAACTGATTGGCATAAATGGCGGAGAACTCCCCGCGCGGCAACACGCCTTTg                                                                                                                                                                      >  1:134611/1‑251 (MQ=255)                                             cAATACGATCTTTGTCTTTTACCGTCGCCATGCGCCAAGTGACGCGCTGTAGATTGTCACCGTATAAATAGGATATGTTGCCCGACAACGTCATGTCACCCTCTTGGGTCAGCACGGTGAATTGCAAAAAACGGCTTTGCGGCGTTTGGCTTACATAATCGGCGATATTGCCTTTAAAt                                                                                                                                                                                                                                           >  1:716408/1‑179 (MQ=255)                                                    atctttgtctttTACCGTCGCCATGCGCCAAGTGACGCGCTGTAGATTGTCACCGTATAAATAGGATATGTTGCCCGACAACGTCATGTCACCCTCTTGGGTCAGCACGGTGAATTGCAAAAAACGGCTTTGCGGCGTTTGGCTTACATAATCGGCGATATTGCCTTTAGATTCCGCCACATCCGCCAGCAACTGATTGGCATAAATGGCGGAGAACTCCCCGCGCGGCAACACGCCTTTGACTTTCAGa                                                                                                                                                             >  1:439897/1‑250 (MQ=255)                                                    atctttgtctttTACCGTCGCCATGCGCCAAGTGACGCGCTGTAGATTGTCACCGTATAAATAGGATATGTTGCCCGACAACGTCATGTCACCCTCTTGGGTCAGCACGGTGAATTGCAAAAAACGGCTTTGCGGCGTTTGGCTTACATAATCGGCGATATTGCCTTTAAAt                                                                                                                                                                                                                                           <  1:708557/172‑1 (MQ=255)                                                    atctttgtctttTACCGTCGCCATGCGCCAAGTGACGCGCTGTAGATTGTCACCGTATAAATAGGATATGTTGCCCGACAACGTCATGTCACCCTCTTGGGTCAGCACGGTGAATTGCAAAAAACGGCTTTGCGGCGTTTGGCTTACATAATCGGCGATATTGCCTTTAAATTCCGCCACATCCGCCAGCAACTGATTGGCATAAATGGCGGAGAACTCCCcgcg                                                                                                                                                                                      >  1:210549/1‑225 (MQ=255)                                                    atctttgtctttTACCGTCGCCATGCGCCAAGTGACGCGCTGTAGATTGTCACCGTATAAATAGGATATGTTGCCCGACAACGTCATGTCACCCTCTTGGGTCAGCACGGTGAATTGCAAAAAACGGCTTTGCGGCGTTTGGCTTACATAATCGGCGATATTGCCTTTAAATTCCGCCACATCCGCCAGCAACTGATTGGCATAAATGGCGGAGAACTCCCCGCGCGGCAACACGCCTTTGACTTTCAGa                                                                                                                                                             <  1:22531/250‑1 (MQ=255)                                                          gtctttTACCGTCGCCATGCGCCAAGTGACGCGCTGTAGATTGTCACCGTATAAATAGGATATGTTGCCCGACAACGTCATGTCACCCTCTTGGGTCAGCACGGTGAATTGCAAAAAACGGCTTTGCGGCGTTTGGCTTACATAATCGGCGATATTGCCTTTAAATTCCGCCACATCCGCCAGCAACTGATTGGCATAAATGGCGGAGAACTCCCCGCGCGGCAACACGCCTTTGACTTTCAGa                                                                                                                                                             >  1:770209/2‑244 (MQ=255)                                                          gtctttTACCGTCGCCATGCGCCAAGTGACGCGCTGTAGATTGTCACCGTATAAATAGGATATGTTGCCCGACAACGTCATGTCACCCTCTTGGGTCAGCACGGTGAATTGCAAAAAACGGCTTTGCGGCGTTTGGCTTACATAATCGGCGATATTGCCTTTAAATTCCGCCACATCCGCCAGCAACTGATTGGCATAAATGGCGGAGAACTCCCCGCGCGGCAACACGCCTTTGACTTTCAGACGCGCaa                                                                                                                                                      >  1:24707/2‑251 (MQ=255)                                                                       gCCATGCGCCAAGTGACGCGCTGTAGATTGTCACCGTATAAATAGGATATGTTGCCCGACAACGTCATGTCACCCTCTTGGGTCAGCACGGTGAATTGCAAAAAACGGCTTTGCGGCGTTTGGCTTACATAATCGGCGATATTGCCTTTAAATTCCGCCACATCCGCCAGCAACTGATTGGCATAAATGGCGGAGAACTCCCCGCGCGGCAACACGCCTTTGACTTTCAGACGCGCAAAAAACGCGTCGGt                                                                                                                                         >  1:593466/1‑251 (MQ=255)                                                                             cgcCAAGTGACGCGCTGTAGATTGTCACCGTATAAATAGGATATGTTGCCCGACAACGTCATGTCACCCTCTTGGGTCAGCACGGTGAATTGCAAAAAACGGCTTTGCGGCGTTTGGCTTACATAATCGGCGATATTGCCTTTAAATTCCGCCACATCCGCCAGCAACTGATTGGCATAAATGGCGGAGAACTCCCCGCGCGGCAACACGCCTTTGACTTTCAGACGCGCAAAAAACGCGTCGGTTtg                                                                                                                                      >  1:537531/1‑248 (MQ=255)                                                                                 aaGTGACGCGCTGTAGATTGTCACCGTATAAATAGGATATGTTGCCCGACAACGTCATGTCACCCTCTTGGGTCAGCACGGTGAATTGCAAAAAACGGCTTTGCGGCGTTTGGCTTACATAATCGGCGATATTGCCTTTAAATTCCGCCACATCCGCCAGCAACTGATTGGCATAAATGGCGGAGAACTCCCCGCGCGGCAACACGCCTTTGACTTTCAGACGCGCAAAAAACGCGTCGGTTTGCTGCTCg                                                                                                                               >  1:35547/1‑251 (MQ=255)                                                                                   gTGACGCGCTGTAGATTGTCACCGTATAAATAGGATATGTTGCCCGACAACGTCATGTCACCCTCTTGGGTCAGCACGGTGAATTGCAAAAAACGGCTTTGCGGCGTTTGGCTTACATAATCGGCGATATTGCCTTTAAATTCCGCCACATCCGCCAGCAACTGATTGGCATAAATGGCGGAGAACTCCCCGCGCGGCAACACGCCTTTGACTTTCAGACGCGCAAAAAACGCGTCGGTTTGCTGCTCGt                                                                                                                              >  1:775657/1‑250 (MQ=255)                                                                                           cTGTAGATTGTCACCGTATAAATAGGATATGTTGCCCGACAACGTCATGTCACCCTCTTGGGTCAGCACGGTGAATTGCAAAAAACGGCTTTGCGGCGTTTGGCTTACATAATCGGCGATATTGCCTTTAAATTCCGCCACATCCGCCAGCAACTGATTGGCATAAATGGCGGAGAACTCCCCGCGCGGCAACACGCCTTTGAc                                                                                                                                                                    <  1:357927/204‑1 (MQ=255)                                                                                             gTAGATTGTCACCGTATAAATAGGATATGTTGCCCGACAACGTCATGTCACCCTCTTGGGTCAGCACGGTGAATTGCAAAAAACGGCTTTGCGGCGTTTGGCTTACATAATCGGCGATATTGCCTTTAAATTCCGCCACATCCGCCAGCAACTGATTGGCATAAATGGCGGAGAACTCCCCGCGCGGCAACACGCCTTTGACTTTCAGACGCGCAAAAAAcg                                                                                                                                                >  1:46753/1‑222 (MQ=255)                                                                                                gATTGTCACCGTATAAATAGGATATGTTGCCCGACAACGTCATGTCACCCTCTTGGGTCAGCACGGTGAATTGCAAAAAACGGCTTTGCGGCGTTTGGCTTACATAATCGGCGATATTGCCTTTAAATTCCGCCACATCCGCCAGCAACTGATTGGCATAAATGGCGGAGAACTCCCCGCGCGGCAACACGCCTTTGACTTTCAGACGCGCAAAAAACGCGTCGGTTTGCTGCTCGTCTGCTTctaacaaa                                                                                                                >  1:12502/1‑244 (MQ=255)                                                                                                  ttGTCACCGTATAAATAGGATATGTTGCCCGACAACGTCATGTCACCCTCTTGGGTCAGCACGGTGAATTGCAAAAAACGGCTTTGCGGCGTTTGGCTTACATAATCGGCGATATTGCCTTTAAATTCCGCCACATCCGCCAGCAACTGATTGGCATAAATGGCGGAGAACTCCCCGCGCGGCAACACGCCTTTGACTTTCAGACGCGCAAAAAACGCGTCGGTTTGCTGCTCGTCTGCTTCTAACAAAt                                                                                                               >  1:348757/1‑250 (MQ=255)                                                                                                  ttGTCACCGTATAAATAGGATATGTTGCCCGACAACGTCATGTCACCCTCTTGGGTCAGCACGGTGAATTGCAAAAAACGGCTTTGCGGCGTTTGGCTTACATAATCGGCGATATTGCCTTTAAATTCCGCCACATCCGCCAGCAACTGATTGGCATAAATGGCGGAGAACTCCCCGCGCGGCAACACGCCTTTGACTTTCAGACGCGCAAAAAACGCGTCGGTTTGCTGCTCGTCTGCTTCTAACAAATc                                                                                                              <  1:417903/251‑1 (MQ=255)                                                                                                        ccGTATAAATAGGATATGTTGCCCGACAACGTCATGTCACCCTCTTGGGTCAGCACGGTGAATTGCAAAAAACGGCTTTGCGGCGTTTGGCTTACATAATCGGCGATATTGCCTTTAAATTCCGCCACATCCGCCAGCAACTGATTGGCATAAATGGCGGAGAACTCCCCGCGCGGCAACACGCCTTTGACTTTCAGACGCGCAAAAAACGCGTCGGTTTGCTGCTCGTCTGCTTCTAACAAATCCGCATg                                                                                                        >  1:708004/1‑251 (MQ=255)                                                                                                        ccGTATAAATAGGATATGTTGCCCGACAACGTCATGTCACCCTCTTGGGTCAGCACGGTGAATTGCAAAAAACGGCTTTGCGGCGTTTGGCTTACATAATCGGCGATATTGCCTTTAAATTCCGCCACATCCGCCAGCAACTGATTGGCATAAATGGCGGAGAACTCCCCGCGCGGCAACACGCCTTTGACTTTCAGACGCGCAAAAAACGCGTCGGTTTGCTGCTCGTCTGCTTCTAACAAATCCGCATg                                                                                                        >  1:26070/1‑251 (MQ=255)                                                                                                         cGTATAAATAGGATATGTTGCCCGACAACGTCATGTCACCCTCTTGGGTCAGCACGGTGAATTGCAAAAAACGGCTTTGCGGCGTTTGGCTTACATAATCGGCGATATTGCCTTTAAATTCCGCCACATCCGCCAGCAACTGATTGGCATAAATGGCGGAGAACTCCCCGCGCGGCAACACGCCTTTGACTTTCAGACGCGCaaaaaa                                                                                                                                                  <  1:32611/208‑1 (MQ=255)                                                                                                           tataAATAGGATATGTTGCCCGACAACGTCATGTCACCCTCTTGGGTCAGCACGGTGAATTGCAAAAAACGGCTTTGCGGCGTTTGGCTTACATAATCGGCGATATTGCCTTTAAATTCCGCCACATCCGCCAGCAACTGATTGGCATAAATGGCGGAGAACTCCCCGCGCGGCAACACGCCTTTGACTTTCAGACGCGCAAAAAACGCGTCGGTTTGCTGCTCGTCTGCTTctaac                                                                                                                   >  1:765415/1‑233 (MQ=255)                                                                                                            ataAATAGGATATGTTGCCCGACAACGTCATGTCACCCTCTTGGGTCAGCACGGTGAATTGCAAAAAACGGCTTTGCGGCGTTTGGCTTACATAATCGGCGATATTGCCTTTAAATTCCGCCACATCCGCCAGCAACTGATTGGCATAAATGGCGGAGAACTCCCcgcgc                                                                                                                                                                                     >  1:96877/1‑170 (MQ=255)                                                                                                                   ggataTGTTGCCCGACAACGTCATGTCACCCTCTTGGGTCAGCACGGTGAATTGCAAAAAACGGCTTTGCGGCGTTTGGCTTACATAATCGGCGATATTGCCTTTAAATTCCGCCACATCCGCCAGCAACTGATTGGCATAAATGGCGGAGAACTCCCCGCGCGGCAACACGCCTTTGACTTTCAGACGCGCAAAAAACGCGTCGGTTTGCTGCTCGTCTGCTTCTAACAAATCCGCATGAATGGCGTAtt                                                                                             >  1:815810/5‑251 (MQ=255)                                                                                                                     ataTGTTGCCCGACAACGTCATGTCACCCTCTTGGGTCAGCACGGTGAATTGCAAAAAACGGCTTTGCGGCGTTTGGCTTACATAATCGGCGATATTGCCTTTAAATTCCGCCACATCCGCCAGCAACTGATTGGCATAAATGGCGGAGAACTCCCCGCGCGGCAACACGCCTTTGACTTTCAGACGCGCAAAAAACGCGTCGGTTTGCTGCTCGTCTGCTTCTAACAAATCCGCATGAATGGCGTAtttt                                                                                           <  1:275291/249‑1 (MQ=255)                                                                                                                      taTGTTGCCCGACAACGTCATGTCACCCTCTTGGGTCAGCACGGTGAATTGCAAAAAACGGCTTTGCGGCGTTTGGCTTACATAATCGGCGATATTGCCTTTAAATTCCGCCACATCCGCCAGCAACTGATTGGCATAAATGGCGGAGAACTCCCCGCGCGGCAACACGCCTTTGACTTTCAGACGCGCAAAAAACGCGTCGGTTTGCTGCTCGTCTGCTTCTAACAAATCCGCATGAATGGCGTAttttt                                                                                          >  1:842781/2‑251 (MQ=255)                                                                                                                       aTGTTGCCCGACAACGTCATGTCACCCTCTTGGGTCAGCACGGTGAATTGCAAAAAACGGCTTTGCGGCGTTTGGCTTACATAATCGGCGATATTGCCTTTAAATTCCGCCACATCCGCCAGCAACTGATTGGCATAAATGGCGGAGAACTCCCCGCGCGGCAACACGCCTTTGACTTTCAGACGCGCAAAAAACGCGTCGGTTTGCTGCTCGTCTGCTTCTAACAAATCCGCATGAATGGCGTATTTTTc                                                                                         <  1:682691/251‑1 (MQ=255)                                                                                                                                    aCGTCATGTCACCCTCTTGGGTCAGCACGGTGAATTGCAAAAAACGGCTTTGCGGCGTTTGGCTTACATAATCGGCGATATTGCCTTTAAATTCCGCCACATCCGCCAGCAACTGAtt                                                                                                                                                                                                                 >  1:442197/1‑118 (MQ=255)                                                                                                                                    aCGTCATGTCACCCTCTTGGGTCAGCACGGTGAATTGCAAAAAACGGCTTTGCGGCGTTTGGCTTACATAATCGGCGATATTGCCTTTAAATTCCGCCACATCCGCCAGCAACTGATTGGCATAAATGGCGGAGAACTCCCCGCGCGGCAACACGCCTTTGACTTTCAGACGCGCAAAAAACGCGTCGGTTtgctg                                                                                                                                   >  1:632286/1‑196 (MQ=255)                                                                                                                                    aCGTCATGTCACCCTCTTGGGTCAGCACGGTGAATTGCAAAAAACGGCTTTGCGGCGTTTGGCTTACATAATCGGCGATATTGCCTTTAAATTCCGCCACATCCGCCAGCAACTGATTGGCATAAATGGCGGAGAACTCCCCGCGCGGCAACACGCCTTTGACTTTCAGACGCGCAAAAAACGCGTCGGTTTGCTGCTCGTCTGCTTCTAACAAATCCGCATGAATGGCGTATTTTTCCAAGGCATTATcc                                                                            <  1:731710/251‑1 (MQ=255)                                                                                                                                    aCGTCATGTCACCCTCTTGGGTCAGCACGGTGAATTGCAAAAAACGGCTTTGCGGCGTTTGGCTTACATAATCGGCGATATTGCCTTTAAATTCCGCCACATCCGCCAGCAACTGATTGGCATAAATGGCGGAGAACTCCCCGCGCGGCAACACGCCTTTGACTTTCAGACGCGCAAAAAACGCGTCGGTTTGCTGCTCGTCTGCTTCTAACAAATCCGCATGAATGGCGTATTTTTCCAAGGCATTATcc                                                                            <  1:370369/251‑1 (MQ=255)                                                                                                                                         aTGTCACCCTCTTGGGTCAGCACGGTGAATTGCAAAAAACGGCTTTGCGGCGTTTGGCTTACATAATCGGCGATATTGCCTTTAAATTCCGCCACATCCGCCAGCAACTGATTGGCATAAATGGCGGAGAACTCCCCGCGCGGCAACACGCCTTTGACTTTCAGACGCGCAAAAAACGCGTCGGTTTGCTGCTCGTCTGCTTCTAACAAATCCGCATGAATGGCGTATTTTTCCAAGGCATTATCCAAgg                                                                        >  1:494861/1‑250 (MQ=255)                                                                                                                                         aTGTCACCCTCTTGGGTCAGCACGGTGAATTGCAAAAAACGGCTTTGCGGCGTTTGGCTTACATAATCGGCGATATTGCCTTTAAATTCCGCCACATCCGCCAGCAACTGATTGGCATAAATGGCGGAGAACTCCCCGCGCGGCAACACGCCTTTGACTTTCAGACGCGCAAAAAACGCGTCGGTTTGCTGCTCGTCTGCTTCTAACAAATCCGCATGAATGGCGTATTTTTCCAAGGCATTATCCAAgg                                                                        >  1:505226/1‑250 (MQ=255)                                                                                                                                         aTGTCACCCTCTTGGGTCAGCACGGTGAATTGCAAAAAACGGCTTTGCGGCGTTTGGCTTACATAATCGGCGATATTGCCTTTAAATTCCGCCACATCCGCCAGCAACTGATTGGCATAAATGGCGGAGAACTCCCCGCGCGGCAACACGCCTTTGACTTTCAGACGCGCAAAAAACGCGTCGGTTTGCTGCTCGTCTGCTTCTAACAAATCCGCATGAATGGCGTATTTTTCCAAGGCATTATCCAAGGc                                                                       <  1:194801/251‑1 (MQ=255)                                                                                                                                               cccTCTTGGGTCAGCACGGTGAATTGCAAAAAACGGCTTTGCGGCGTTTGGCTTACATAATCGGCGATATTGCCTTTAAATTCCGCCACATCCGCCAGCAACTGATTGGCATAAATGGCGGAGAACTCCCCGCGCGGCAACACGCCTTTGACTTTCAGACGCGCAAAAAACGCGTCGGTTTGCTGCTCGTCTGCTTCTAACAAATCCGCATGAATGGCGTATTTTTCCAAGGCATTATCCAAGGCGAAAtt                                                                 >  1:133118/1‑251 (MQ=255)                                                                                                                                               cccTCTTGGGTCAGCACGGTGAATTGCAAAAAACGGCTTTGCGGCGTTTGGCTTACATAATCGGCGATATTGCCTTTAAATTCCGCCACATCCGCCAGCAACTGATTGGCATAAATGGCGGAGAACTCCCCGCGCGGCAAAACGCCTTTCACTTTCAGACGCGCAAAAAACGCGCCGGTCTGCTGCTCGTCTCCTTCTAACAAATCCGCATGAATGGCGTATTTTTCCAAGGCATTATCCAAGGCGAAAtt                                                                 >  1:353928/1‑251 (MQ=255)                                                                                                                                                 ctctTGGGTCAGCACGGTGAATTGCAAAAAACGGCTTTGCGGCGTTTGGCTTACATAATCGGCGATATTGCCTTTAAATTCCGCCACATCCGCCAGCAACTGATTGGCAt                                                                                                                                                                                                            >  1:47740/1‑110 (MQ=255)                                                                                                                                                 ctctTGGGTCAGCACGGTGAATTGCAAAAAACGGCTTTGCGGCGTTTGGCTTACATAATCGGCGATATTGCCTTTAAATTCCGCCACATCCGCCAGCAACTGATTGGCATAAATGGCGGAGAACTCCCCGCGCGGCAACACGCCTTTGACTTTCAGACGCGCAAAAAACGCGTCGGTTTGCTGCTCGTCTGCTTctaac                                                                                                                   <  1:62796/199‑5 (MQ=255)                                                                                                                                                  tctTGGGTCAGCACGGTGAATTGCAAAAAACGGCTTTGCGGCGTTTGGCTTACATAATCGGCGATATTGCCTTTAAATTCCGCCACATCCGCCAGCAACTGATTGGCATAAATGGCGGAGAACTCCCCGCGCGGCAACACGCCTTTGACTTTCAGACGCGCAAAAAACGCGTCGGTTTGCTGCTCGTCTGCTTCTAACAAATCCGCATGAATGGCGTATTTTTCCAAGGCATTATCCAAGGCGAAAtttt                                                               >  1:235087/1‑250 (MQ=255)                                                                                                                                                       ggTCAGCACGGTGAATTGCAAAAAACGGCTTTGCGGCGTTTGGCTTACATAATCGGCGATATTGCCTTTAAATTCCGCCACATCCGCCAGCAACTGATTGGCATAAATGGCGGAGAACTCCCCGCGCGGCAACACGCCTTTGACTTTCAGACGCGCAAAAAACGCGTCGGTTTGCTGCTCGTCTGCTTCTAACAAATCCGCATGAATGGCGTATTTTTCCAAGGCATTATCCAAGGCGAAATTTTCGCtat                                                         >  1:371472/1‑249 (MQ=255)                                                                                                                                                       ggTCAGCACGGTGAATTGCAAAAAACGGCTTTGCGGCGTTTGGCTTACATAATCGGCGATATTGCCTTTAAATTCCGCCACATCCGCCAGCAACTGATTGGCATAAATGGCGGAGAACTCCCCGCGCGGCAACACGCCTTTGACTTTCAGACGCGCAAAAAACGCGTCGGTTTGCTGCTCGTCTGCTTCTAACAAATCCGCATGAATGGCGTATTTTTCCAAGGCATTATCCAAGGCGAAATTTTCGCtat                                                         >  1:262407/1‑249 (MQ=255)                                                                                                                                                       ggTCAGCACGGTGAATTGCAAAAAACGGCTTTGCGGCGTTTGGCTTACATAATCGGCGATATTGCCTTTAAATTCCGCCACATCCGCCAGCAACTGATTGGCATAAATGGCGGAGAACTCCCCGCGCGGCAACACGCCTTTGACTTTCAGACGCGCAAAAAACGCGTCGGTTTGCTGCTCGTCTGCTTCTAACAAATCCGCATGAATGGCGTATTTTTCCAAGGCATTATCCAAGGCGAAATTTTCGCtat                                                         >  1:896817/1‑249 (MQ=255)                                                                                                                                                            gCACGGTGAATTGCAAAAAACGGCTTTGCGGCGTTTGGCTTACATAATCGGCGATATTGCCTTTAAATTCCGCCACATCCGCCAGCAACTGATTGGCATAAATGGCGGAGAACTCCCCGCGCGGCAACACGCCTTTGACTTTCAGACGCGCAAAAAACGCGTCGGTTTGCTGCTCGTCTGCTTCTAACAAATCCGCATGAATGGCGTATTTTTcc                                                                                        <  1:805448/215‑1 (MQ=255)                                                                                                                                                                   gAATTGCAAAAAACGGCTTTGCGGCGTTTGGCTTACATAATCGGCGATATTGCCTTTAAATTCCGCCACATCCGCCAGCAACTGATTGGCATAAATGGCGGAGAACTCCCCGCGCGGCAACACGCCTTTGACTTTCAGACGCGCAAAAAACGCGTCGGTTTGCTGCTCGTCTGCTTCTAACAAATCCGCATGAATGGCGTATTTTTCCAAGGCATTATCCAAGGCGAAATTTTCGCTATCGGCAATGCttt                                             >  1:407508/1‑251 (MQ=255)                                                                                                                                                                       tGCAAAAAACGGCTTTGCGGCGTTTGGCTTACATAATCGGCGATATTGCCTTTAAATTCCGCCACATCCGCCAGCAACTGATTGGCATAAATGGCGGAGAACTCCCCGCGCGGCAACACGCCTTTGACTTTCAGACGCGCAAAAAACGCGTCGGTTTGCTGCTCGTCTGCTTCTAACAAATCCGCATGAATGGCGTATTTTTCCAAGGCATTATCCAAGGCGAAATTTTCGCTATCGGCAATGCTttcttc                                         <  1:643523/251‑1 (MQ=255)                                                                                                                                                                                  gCTTTGCGGCGTTTGGCTTACATAATCGGCGATATTGCCTTTAAATTCCGCCACATCCGCCAGCAACTGATTGGCATAAATGGCGGAGAACTCCCCGCGCGGCAACACGCCTTTGACTTTCAGACGCGCAAAAAACGCGTCGGTTTGCTGCTCGTCTGCTTCTAACAAATCCGCATGAATGGCGTATTTTTCCAAGGCATTATCCAAGGCGAAAtt                                                                 <  1:27679/216‑1 (MQ=255)                                                                                                                                                                                             tttGGCTTACATAATCGGCGATATTGCCTTTAAATTCCGCCACATCCGCCAGCAACTGATTGGCATAAATGGCGGAGAACTCCCCGCGCGGCAACACGCCTTTGACTTTCAGACGCGCAAAAAACGCGTCGGTTTGCTGCTCGTCTGCTTCTAACAAATCCGCATGAATGGCGTATTTTTCCAAGGCATTATCCAAGGCGAAATTTTCGCTATCGGCAATGCTTTCTTCCTGTTCGGAGAAATTCACGcc                    <  1:824162/250‑1 (MQ=255)                                                                                                                                                                                                ggCTTACATAATCGGCGATATTGCCTTTAAATTCCGCCACATCCGCCAGCAACTGATTGGCATAAATGGCGGAGAACTCCCCGCGCGGCAACACGCCTTTGACTTTCAGACGCGCAAAAAACGCGTCGGTTTGCTGCTCGTCTGCTTCTAACAAATCCGCATGAATGGCGTATTTTTCCAAGGCATTATCCAAGGCGAAATTTTCGCTATCGGCAATGCTTTCTTCCTGTTCGGAGAAATTCACGCCAAgg                >  1:77054/1‑251 (MQ=255)                                                                                                                                                                                                       aTAATCGGCGATATTGCCTTTAAATTCCGCCACATCCGCCAGCAACTGATTGGCATAAATGGCGGAGAACTCCCCGCGCGGCAACACGCCTTTGACTTTCAGACGCGCAAAAAACGCGTCGGTTTGCTGCTCGTCTGCTTCTAACAAATCCGCATGAATGGCGTATTTTTCCAAGGCATTATCCAAGGCGAAATTTTCGCTATCGGCAATGCTTTCTTCCTGTTCGGAGAAATTCACGCCAAGGCGttttt         <  1:493412/251‑1 (MQ=255)                                                                                                                                                                                                           tCGGCGATATTGCCTTTAAATTCCGCCACATCCGCCAGCAACTGATTGGCATAAATGGCGGAGAACTCCCCGCGCGGCAACACGCCTTTGACTTTCAGACGCGCAAAAAACGCGTCGGTTTGCTGCTCGTCTGCTTCTAACAAATCCGCATGAATGGCGTATTTTTCCAAGGCATTATCCAAGGCGAAATTTTCGCTATCGGCAATGCTTTCTTCCTGTTCGGAGAAATTCACGCCAAGGCGTTTTTCaaa     >  1:854633/1‑251 (MQ=255)                                                                                                                                                                                                              gCGATATTGCCTTTAAATTCCGCCACATCCGCCAGCAACTGATTGGCATAAATGGCGGAGAACTCCCCGCGCGGCAACACGCCTTTGACTTTCAGACGCGCAAAAAACGCGTCGGTTTGCTGCTCGTCTGCTTCTAACAAATCCGCATGAATGGCGTATTTTTCCAAGGCATTATCCAAGGCGAAATTTTCGCTATCGGCAATGCTTTCTTcc                                        <  1:520978/213‑1 (MQ=255)                                                                                                                                                                                                              gCGATATTGCCTTTAAATTCCGCCACATCCGCCAGCAACTGATTGGCATAAATGGCGGAGAACTCCCCGCGCGGCAACACGCCTTTGACTTTCAGACGCGCAAAAAACGCGTCGGTTTGCTGCTCGTCTGCTTCTAACAAATCCGCATGAATGGCGTATTTTTCCAAGGCATTATCCAAGGCGAAATTTTCGCTATCGGCAATGCTTTCTTCCTGTTCGGAGAAATTCACGCCAAGGCGTTTTTCAAAGaa  >  1:793173/1‑251 (MQ=255)                                                                                                                                                                                                                       ccTTTAAATTCCGCCACATCCGCCAGCAACTGATTGGCATAAATGGCGGAGAACTCCCCGCGCGGCAACACGCCTTTGACTTTCAGACGCGCAAAAAACGCGTCGGTTTGCTGCTCGTCTGCTTCTAACAAATCCGCATGAATGGCGTATTTTTCCAAGGCATTATCCAAGGCGAAATTTTCGCTATCGGCAAt                                                  <  1:819985/194‑1 (MQ=255)                                                                                                                                                                                                                            |                                                                                                                                                                                                                                             CTGTTCCTGCGTGGCGCACAACAATAAGTGATAAAGCCAAGCTTCAATACGATCTTTATCTTTTACCGTCGCCATGCGCCAAGTGACGCGCTGTAGATTGTCACCGTATAAATGGGAAATGTTGCCCGACAACGTCATGTCACCCTCTTGGGTCGGCACGGTGAATTGCACAAAACGGCTTTGCGGCGTTTGGCTTACATAATCGGCGATCTTGCCTTTGAATTCCGCCACATCCGCCAGCAACTGATTGGCATAAATGGCGGAGAACTCCCCGCGCGGCAACACGCCTTTGACTTTCAGACGCGCAAAAAACGCGTCGGTTTGCTGCTCGTCCGCTTCCACCAAATCCGCATGAATGGCGTATTTTTCCAAGGCATTATCCAAGGCGAAATTTTCGCTGTCGGCAATGCTTTCTTCCTGTTCGGAGAAATTCACGCCAAGGCGTTTTTCAAAGAA  >  1/1830702‑1831157

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑