Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,830,741	A→G	intergenic (+48841/+4643)	unknown → / ← unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,830,741	0	A	G	100.0%	225.9 / NA	75	intergenic (+48841/+4643)	unknown/unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (33/42);  total (33/42)

GGAAAAACACTTGTCAATATCCACCGCACTTTCATCGGCAATCAACTTCAAATACGCTTCAAGGTTTGTGGTCGGAACCAGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGCAGTTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCACAACAATAAGTGATAAAGCCAAGCTTCAATACGATCTTTATCTTTTACCGTCGCCATGCGCCAAGTGACGCGCTGTAGATTGTCACCGTATAAATGGGAAATGTTGCCCGACAACGTCATGTCACCCTCTTGGGTCGGCACGGTGAATTGCACAAAACGGCTTTGCGGCGTTTGGCTTACATAATCGGCGATCTTGCCTTTGAATTCCGCCACATCCGCCAGCAACTGATTGGCATAAATGGCGGAGAACTCCCCGCGCGGCAACA  >  1/1830495‑1830985                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                     ggAAAAACACTTGTCAACATCCACCGCACTTTCATCGGCAATCAGCTTCAAATACGCTTCAAGGTTTGTGGTCGGAATAAGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCt                                                                                                                                                                                                                                                   <  1:304416/250‑1 (MQ=255)       acacTTGTCAACATCCACCGCACTTTCATCGGCAATCAGCTTCAAATACGCTTCAAGGTTTGTGGTCGGAATAAGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATAc                                                                                                                                                                                                                                            <  1:394984/251‑1 (MQ=255)       acacTTGTCAACATCCACCGCACTTTCATCGGCAATCAGCTTCAAATACGCTTCAAGGTTTGTGGTCGGAATAAGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATAc                                                                                                                                                                                                                                            <  1:36619/251‑1 (MQ=255)       acacTTGTCAACATCCACCGCACTTTCATCGGCAATCAGCTTCAAATACGCTTCAAGGTTTGTGGTCGGAATAAGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATAc                                                                                                                                                                                                                                            <  1:328459/251‑1 (MQ=255)            tGTCAACATCCACCGCACTTTCATCGGCAATCAGCTTCAAATACGCTTCAAGGTTTGTGGTCGGAATAAGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATACGatct                                                                                                                                                                                                                                       <  1:16359/251‑1 (MQ=255)            tGTCAACATCCACCGCACTTTCATCGGCAATCAGCTTCAAATACGCTTCAAGGTTTGTGGTCGGAATAAGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATACGatct                                                                                                                                                                                                                                       <  1:227502/251‑1 (MQ=255)            tGTCAACATCCACCGCACTTTCATCGGCAATCAGCTTCAAATACGCTTCAAGGTTTGTGGTCGGAATAAGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATACGat                                                                                                                                                                                                                                         >  1:198765/1‑249 (MQ=255)                 acatCCACCGCACTTTCATCGGCAATCAGCTTCAAATACGCTTCAAGGTTTGTGGTCGGAATAAGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATACGatctttgtc                                                                                                                                                                                                                                  <  1:257595/249‑4 (MQ=255)                    tCCACCGCACTTTCATCGGCAATCAGCTTCAAATACGCTTCAAGGTTTGTGGTCGGAATAAGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATACGatctttgtcttt                                                                                                                                                                                                                               >  1:71343/1‑251 (MQ=255)                       aCCGCACTTTCATCGGCAATCAGCTTCAAATACGCTTCAAGGTTTGTGGTCGGAATAAGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATACGATCTTTGTCTTTTAc                                                                                                                                                                                                                            <  1:35369/251‑1 (MQ=255)                               ttCATCGGCAATCAGCTTCAAATACGCTTCAAGGTTTGTGGTCGGAATAAGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATACGatct                                                                                                                                                                                                                                       >  1:614990/1‑232 (MQ=255)                               ttCATCGGCAATCAGCTTCAAATACGCTTCAAGGTTTGTGGTCGGAATAAGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATACGATCTTTGTCTTTTACCGTCGCCa                                                                                                                                                                                                                    >  1:44270/1‑251 (MQ=255)                               ttCATCGGCAATCAGCTTCAAATACGCTTCAAGGTTTGTGGTCGGAATAAGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATACGATCTTTGTCTTTTACCGTCGCCa                                                                                                                                                                                                                    >  1:875909/1‑251 (MQ=255)                                    cGGCAATCAGCTTCAAATACGCTTCAAGGTTTGTGGTCGGAATAAGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATACGATCTTTGTCTTTTACCGTCGCCATgcgc                                                                                                                                                                                                               <  1:787968/251‑1 (MQ=255)                                       cAATCAGCTTCAAATACGCTTCAAGGTTTGTGGTCGGAATAAGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATACGATCTTTGTCTTTTACCGTCGCCATGCGCCaa                                                                                                                                                                                                            >  1:202204/1‑251 (MQ=255)                                          tcagcTTCAAATACGCTTCAAGGTTTGTGGTCGGAATAAGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATACGATCTTTGTCTTTTACCGTCGCCATGCGCCAAGTg                                                                                                                                                                                                         <  1:233613/247‑1 (MQ=255)                                             gcTTCAAATACGCTTCAAGGTTTGTGGTCGGAATAAGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATACGATCTTTGTCTTTTACCGTCGCCATGCGCCAAGTGAcg                                                                                                                                                                                                      >  1:516477/2‑251 (MQ=255)                                             gcTTCAAATACGCTTCAAGGTTTGTGGTCGGAATAAGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATACGATCTTTGTCTTTTACCGTCGCCATGCGCCAAGTGAcg                                                                                                                                                                                                      >  1:365544/2‑251 (MQ=255)                                             gcTTCAAATACGCTTCAAGGTTTGTGGTCGGAATAAGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATACGATCTTTGTCTTTTACCGTCGCCATGCGCCAAGTGAcg                                                                                                                                                                                                      >  1:861918/2‑251 (MQ=255)                                                         cTTCAAGGTTTGTGGTCGGAATAAGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATACGATCTTTGTCTTTTACCGTCGCCATGCGCCAAGTGACGCGCTGTAGATTg                                                                                                                                                                                          >  1:856207/1‑251 (MQ=255)                                                         cTTCAAGGTTTGTGGTCGGAATAAGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGGTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGGCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATACGATCTTTGTCTTTTACCGTCGCCATGCGCCAAGTGACGCGCTGTAGAtt                                                                                                                                                                                           <  1:414920/250‑1 (MQ=255)                                                           tCAAGGTTTGTGGTCGGAATAAGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATACGATCTTTGTCTTTTACCGTCGCCATGCGCCAAGTGACGCGCTGTAGATTGTc                                                                                                                                                                                        <  1:857408/251‑1 (MQ=255)                                                           tCAAGGTTTGTGGTCGGAATAAGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATACGATCTTTGTCTTTTACCGTCGCCATGCGCCAAGTGACGCGCTGTAGATTGTc                                                                                                                                                                                        <  1:692905/251‑1 (MQ=255)                                                           tCAAGGTTTGTGGTCGGAATAAGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATACGATCTTTGTCTTTTACCGTCGCCATGCGCCAAGTGACGCGCTGTAGATTGTc                                                                                                                                                                                        <  1:788160/251‑1 (MQ=255)                                                           tCAAGGTTTGTGGTCGGAATAAGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATACGATCTTTGTCTTTTACCGTCGCCATGCGCCAAGTGACGCGCTGTAGATTGTc                                                                                                                                                                                        <  1:354445/251‑1 (MQ=255)                                                               ggTTTGTGGTCGGAATAAGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATACGATCTTTGTCTTTTACCg                                                                                                                                                                                                                          >  1:844337/1‑213 (MQ=255)                                                               ggTTTGTGGTCGGAATAAGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATACGATCTTTGTCTTTTACCGTCGCCATGCGCCAAGt                                                                                                                                                                                                          >  1:297581/1‑229 (MQ=255)                                                                       gTCGGAATAAGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATACGATCTTTGTCTTTTACCGTCGCCATGCGCCAAGTGACGCGCTGTAGATTGTCACCGTATAAAt                                                                                                                                                                             <  1:692636/250‑1 (MQ=255)                                                                           gaataaGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACCTTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATACGATCTTTGTCTTTTACCGTCGCCATGCGCCAAGTGACGCGCTCTAGATTGTCACCGTATAAATAGGat                                                                                                                                                                        >  1:152824/6‑250 (MQ=255)                                                                           gaataaGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATACGATCTTTGTCTTTTACCGTCGCCATGCGCCAAGTGACGCGCTGTAGa                                                                                                                                                                                             <  1:386428/225‑1 (MQ=255)                                                                           gaataaGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATACGATCTTTGTCTTTTACCGTCGCCATGCGCCAAGTGACGCGCTGTAGa                                                                                                                                                                                             <  1:285260/225‑1 (MQ=255)                                                                           gaataaGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATACGATCTTTGTCTTTTACCGTCGCCATGCGCCAAGTGACGCGCTGTAGATTGTCACCGTATAAATAGGat                                                                                                                                                                        >  1:493806/6‑250 (MQ=255)                                                                               aaGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATACGATCTTTGTCTTTTACCGTCGCCATGCGCCAAGTGACGCGCTGTAGATTGTCACCGTATAAATAGGATATGt                                                                                                                                                                    <  1:586465/250‑1 (MQ=255)                                                                                  cTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATACGATCTTTGTCTTTTACCGTCGCCATGCGCCAAGTGACGCGCTGTAGATTGTCACCGTATAAATAGGATATGt                                                                                                                                                                    <  1:851581/248‑1 (MQ=255)                                                                                    gTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATACGatc                                                                                                                                                                                                                                        <  1:245305/178‑1 (MQ=255)                                                                                      aaTTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATACGATCTTTGTCTTTTACCGTCGCCATGCGCCAAGTGACGCGCTGTAGATTGTCACCGTATAAATAGGATATg                                                                                                                                                                     >  1:180634/1‑243 (MQ=255)                                                                                                      ccAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATACGatctttgtct                                                                                                                                                                                                                                 <  1:635883/167‑1 (MQ=255)                                                                                                      ccAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATACGat                                                                                                                                                                                                                                         <  1:69528/159‑1 (MQ=255)                                                                                                      ccAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATACGATCTTTGTCTTTTACCGTCGCCATGCGCCAAGTGACGCGCTGTAGATTGTCACCGTATAAAt                                                                                                                                                                             <  1:832427/219‑1 (MQ=255)                                                                                                             aCTTTCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATACGATCTTTGTCTTTTACCGTCGCCATGCGCCAAGTGACGCGCTGTAGATTGTCACCGTTTAACTAGGATATGTTGCCCGACAACGTCATGTCACCCTCTTggg                                                                                                                                      <  1:430409/251‑1 (MQ=255)                                                                                                               tttCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATACGATCTTTGTCTTTTACCGTCGCCATGCGCCAAGTGACGCGCTGTAGATTGTCACCGTATAAATAGGATATGTTGCCCGACAACGTCATGTCACCCTCTTGGGTc                                                                                                                                    <  1:534107/251‑1 (MQ=255)                                                                                                                    acaTAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATACGATCTTTGTCTTTTACCGTCGCCATGCGCCAAGTGACGCGCTGTAGATTGTCACCGTATAAATAGGATATGTTGCCCGACAACGTCATGTCACCCTCTTGGGTCAGCAc                                                                                                                               <  1:708341/251‑1 (MQ=255)                                                                                                                    acaTAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATACGATCTTTGTCTTTTACCGTCGCCATGCGCCAAGTGACGCGCTGTAGATTGTCACCGTATAAATAGGATATGTTGCCCGACAACGTCATGTCACCCTCTTGGGTCAGCAc                                                                                                                               <  1:234999/251‑1 (MQ=255)                                                                                                                         aaTTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATACGATCTTTGTCTTTTACCGTCGCCATGCGCCAAGTGACGCGCTGTAGATTGTCACCGTATAAAtag                                                                                                                                                                           >  1:669037/1‑200 (MQ=255)                                                                                                                         aaTTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATACGATCTTTGTCTTTTACCGTCGCCATGCGCCAAGTGACGCGCTGTAGATTGTCACCGTATAAATAGGATATGTTGCCCGACAACGTCATGTCACCCTCTTGGGTCAGCACGGTGa                                                                                                                          >  1:651069/1‑251 (MQ=255)                                                                                                                          aTTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATACGATCTTTGTCTTTTACCGTCGCCATGCGCCAAGTGACGCGCTGTAGATTGTCACCGTATAAATAGGATATGTTGCCCGACAACGTCATGTCACCCTCTTGGGTc                                                                                                                                    >  1:601763/1‑240 (MQ=255)                                                                                                                               tagctGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATACGATCTTTGTCTTTTACCGTCGCCATGCGCCAAGTGACGCGCTGTAGATTGTCACCGTATAAATAGGATATGTTGCCCGACAACGTCATGTCACCCTCTTGGGTCAGCACGGTGAATTGca                                                                                                                    >  1:840732/5‑251 (MQ=255)                                                                                                                                    gggCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATACGatc                                                                                                                                                                                                                                        >  1:897279/1‑130 (MQ=255)                                                                                                                                    gggCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATACGATCTTTGTCTTTTACCGTCGCCATGCGCCAAGTGACGCGCTGTAGATTGTCAc                                                                                                                                                                                      >  1:892192/1‑180 (MQ=255)                                                                                                                                    gggCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATACGATCTTTGTCTTTTACCGTCGCCATGCGCCAAGTGACGCGCTGTAGATTGTCACCGTATAAATAGGATATGt                                                                                                                                                                    <  1:886975/198‑1 (MQ=255)                                                                                                                                    gggCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATACGATCTTTGTCTTTTACCGTCGCCATGCGCCAAGTGACGCGCTGTAGATTGTCACCGTATAAATAGGATATGTTGCCCGACAACGTCATGTCACCCTCTTGGGTCAGCACGGTGAAtt                                                                                                                       >  1:695984/1‑243 (MQ=255)                                                                                                                                        aaCGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATACGATCTTTGTCTTTTACCGTCGCCATGCGCCAAGTGACGCGCTGTAGATTGTCACCGTATAAATAGGATATGTTGCCCGACAACGTCATGTCACCCTCTTGGGTCAGCACGGTGAATTGCAAAAAACGGc                                                                                                           <  1:56692/251‑1 (MQ=255)                                                                                                                                                   gTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATACGATCTTTGTCTTTTACCGTCGCCATGCGCCAAg                                                                                                                                                                                                           <  1:115719/144‑1 (MQ=255)                                                                                                                                                            acacATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATACGATCTTTGTCTTTTACCGTCGCCATGCGCCAAGTGACGCGCTGTAGATTGTCACCGTATAAATAGGATATGTTGCCCGACAACGTCATGTCACCCTCTTGGGTCAGCACGGTGAATTGCAAAAAACGGCTTTGCGGCGTTTGGCTTACa                                                                                       >  1:780174/1‑251 (MQ=255)                                                                                                                                                             cacaTTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATACGATCTTTGTCTTTTACCGTCGCCATGCGCCAAGTGACGCGCTGTAGATTGTCACCGTATAAATAGGATATGTTGCCCGACAACGTCATGTCACCCTCTTGGGTCAGCACGGTGAATTGCAAAAAACGGCTTTGCGGCGTTTGGCTTACAt                                                                                      <  1:227179/251‑1 (MQ=255)                                                                                                                                                                            gcgcTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATACGATCTTTGTCTTTTACCGTCGCCATGCGCCAAGTGACGCGCTGTAGATTGTCACCGTATAAATAGGATATGTTg                                                                                                                                                                  >  1:90000/1‑160 (MQ=255)                                                                                                                                                                            gcgcTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATACGATCTTTGTCTTTTACCGTCGCCATGCGCCAAGTGACGCGCTGTAGATTGTCACCGTATAAATAGGATATGTTGCCCGACAACGTCATGTCACCCTCTTGGGTCAGCACGGTGAATTGCAAAAAACGGCTTTGCGGCGTTTGGCTTACATAATCGGCGATATTg                                                                        >  1:745974/1‑250 (MQ=255)                                                                                                                                                                             cgcTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATAc                                                                                                                                                                                                                                            <  1:480062/85‑1 (MQ=255)                                                                                                                                                                             cgcTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATACGATCTTTGTCTTTTACCGTCGCCATGCGCCAAGTGACGCGCTGTAGATTGTCACCGTATAAATAGGATATGTTGCCCGACAACGTCATGTCACCCTCTTGGGTCAGCACGGTGAATTGCAAAAAACGGCTTTGCGGCGTTTGGCTTACATa                                                                                     >  1:437311/1‑236 (MQ=255)                                                                                                                                                                             cgcTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATACGATCTTTGTCTTTTACCGTCGCCATGCGCCAAGTGACGCGCTGTAGATTGTCACCGTATAAATAGGATATGTTGCCCGACAACGTCATGTCACCCTCTTGGGTCAGCACGGTGAATTGCAAAAAACGGCTTTGCGGCGTTTGGCTTACATAATCGGCGATATTGcc                                                                      <  1:687693/251‑1 (MQ=255)                                                                                                                                                                                 gATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATACGATCTTTGTCTTTTACCGTCGCCATGCGCCAAGTGACGCGCTGTAGATTGTCACCGTATAAAta                                                                                                                                                                            >  1:44320/1‑144 (MQ=255)                                                                                                                                                                                 gATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATACGATCTTTGTCTTTTACCGTCGCCATGCGCCAAGTGACGCGCTGTAGATTGTCACCGTATAAATAGGATATGTTGCCCGACAACGTCATGTCACCCTCTTGGGTCAGCACGGTGa                                                                                                                          <  1:331539/195‑1 (MQ=255)                                                                                                                                                                                 gATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATACGATCTTTGTCTTTTACCGTCGCCATGCGCCAAGTGACGCGCTGTAGATTGTCACCGTATAAATAGGATATGTTGCCCGACAACGTCATGTCACCCTCTTGGGTCAGCACGGTGAATTGCAAAAAACGGCTTTGCGGCGTTTGGCTTACATAATCGGCGATATTGCCt                                                                     <  1:898568/248‑1 (MQ=255)                                                                                                                                                                                   tCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATACGATCTTTGTCTTTTACCGTCGCCATGCGCCAAGTGACGCGCTGTAGATTGTCACCGTATAAATAGGATATGTTGCCCGACAACGTCATGTCACCCTCTTGGGTCAGCACGGTGAATTGCAAAAAACGGCTTTGCGGCGTTTGGCTTACATAATCGGCGATATTGCCtttaaa                                                                >  1:759650/1‑248 (MQ=255)                                                                                                                                                                                                            ctacagcgcgtcacttGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATACGATCTTTGTCTTTTACCGTCGCCATGCGCCAAGTGACGCGCTGTAGATTGTCACCGTATAAATAGGATATGTTGCCCGACAACGTCATGTCGCCCTCTTggg                                                                                                                                      <  1:603390/141‑1 (MQ=255)                                                                                                                                                                                                             cGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATACGATCTTTGTCTTTTACCGTCGCCATGCGCCAAGTGACGCGCTGTAGATTGTCACCGTATAAATAGGATATGTTGCCCGACAACGTCATGTCACCCTCTTGGGTCAGCACGGTGAATTGCAAAAAACGGCTTTGCGGCGTTTGGCTTACAt                                                                                      <  1:344999/203‑1 (MQ=255)                                                                                                                                                                                                                cTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATACGATCTTTGTCTTTTACCGTCGCCATGCGCCAAGTGACGCGCTGTAGATTGTCACCGTATAAATAGGATATGTTGCCCGACAACGTCATGTCACCCTCTTGGGTCAGCACGGTGAATTGCAAAAAACGGCTTTGCGGCGTTTGGCTTACATAATCGGCGATATTGCCTTTAAATTCCGCCACATCCGCCAGCAACTGATTgg                                   >  1:891271/1‑251 (MQ=255)                                                                                                                                                                                                                  gTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATACGATCTTTGTCTTTTACCGTCGCCATGCGCCAAGTGACGCGCTGTAGATTGTc                                                                                                                                                                                        >  1:253886/1‑100 (MQ=255)                                                                                                                                                                                                                  gTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATACGATCTTTGTCTTTTACCGTCGCCATGCGCCAAGTGACGCGCTGTAGATTGTCACCGTATAAATAGGATATGTTGCCCGACAACGTCATGTCACCCTCTTGGGTCAGCAc                                                                                                                               <  1:583182/157‑1 (MQ=255)                                                                                                                                                                                                                  gTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATACGATCTTTGTCTTTTACCGTCGCCATGCGCCAAGTGACGCGCTGTAGATTGTCACCGTATAAATAGGATATGTTGCCCGACAACGTCATGTCACCCTCTTGGGTCAGCACGGTGAATTGCAAAAAACGGCTTTGCGGCGTTTGGc                                                                                            >  1:9579/1‑192 (MQ=255)                                                                                                                                                                                                                             gcgcATAACAATAAGTGATAAAGCCAGGCTTCAATACGATCTTTGTCTTTTACCGTCGCCATGCGCCAAGTGACGCGCTGTAGATTGTCACCGTATAAATAGGATATGTTGCCCGACAACGTCATGTCACCCTCTTGGGTCAGCACGGTGAATTGCAAAAAACGGCTTTGCGGCGTTTGGCTTACATAATCGGCGATATTGCCtttaaa                                                                <  1:358188/209‑4 (MQ=255)                                                                                                                                                                                                                                 ataacaaTAAGTGATAAAGCCAGGCTTCAATACGATCTTTGTCTTTTACCGTCGCCATGCGCCAAGTGACGCGCTGTAGATTGTCACCGTATAAATAGGATATGTTGCCCGACAACGTCATGTCACCCTCTTGGGTCAGCACGGTGAATTGCAAAAAACGGCTTTGCGGCGTTTGGCTTACATAATCGGCGATATTGCCTTTAAATTCCGCCACATCCGCCAGCAACTGATTGGCATAAATGGCGGAGAAc                  <  1:270001/249‑1 (MQ=255)                                                                                                                                                                                                                                           gTGATAAAGCCAGGCTTCAATACGATCTTTGTCTTTTACCGTCGCCATGCGCCAAGTGACGCGCTGTAGATTGTCACCGTATAAATAGGATATGTCGCCCGACAACGTCATGTCACCCTCTTGGGTCAGCACGGTGAATTGCAAAAAACGGCTTTGCGGCGTTTGGCTTACATAATCGGCGATATTGCCTTTAAATTCCGCCACATCCGCCAGCAACTGATTGGCATAAATGGCGGAGAACTCCCcgcgcg        >  1:223632/1‑251 (MQ=255)                                                                                                                                                                                                                                            tGATAAAGCCAGGCTTCAATACGATCTTTGTCTTTTACCGTCGCCATGCGCCAAGTGACGCGCTGTAGATTGTCACCGTATAAATAGGATATGTTGCCCGACAACGTCATGTCACCCTCTTGGGTCAGCACGGTGAATTGCAAAAAACGGCTTTGCGGCGTTTGGCTTACATAATCGGCGATATTGCCTTTAAATTCCGCCACATCCGCCAGCAACTGATTGGCATAAATGGCGGAGAACTCCCcgcgc         <  1:17493/249‑1 (MQ=255)                                                                                                                                                                                                                                                  aGCCAGGCTTCAATACGATCTTTGTCTTTTACCGTCGCCATGCGCCAAGTGACGCGCTGTAGATTGTCACCGTATAAATAGGATATGTTGCCCGACAACGTCATGTCACCCTCTTGGGTCAGCACGGTGAATTGCAAAAAACGGCTTTGCGGCGTTTGGCTTACATAATCGGCGATATTGCCTTTAAATTCCGCCACATCCGCCAGCAACTGATTGGCATAAATGGCGGAGAACTCCCCGCGCGGCAaca  >  1:893814/1‑250 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                     GGAAAAACACTTGTCAATATCCACCGCACTTTCATCGGCAATCAACTTCAAATACGCTTCAAGGTTTGTGGTCGGAACCAGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGCAGTTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCACAACAATAAGTGATAAAGCCAAGCTTCAATACGATCTTTATCTTTTACCGTCGCCATGCGCCAAGTGACGCGCTGTAGATTGTCACCGTATAAATGGGAAATGTTGCCCGACAACGTCATGTCACCCTCTTGGGTCGGCACGGTGAATTGCACAAAACGGCTTTGCGGCGTTTGGCTTACATAATCGGCGATCTTGCCTTTGAATTCCGCCACATCCGCCAGCAACTGATTGGCATAAATGGCGGAGAACTCCCCGCGCGGCAACA  >  1/1830495‑1830985

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑