Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,830,624	T→C	intergenic (+48724/+4760)	unknown → / ← unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,830,624	0	T	C	100.0%	229.6 / NA	76	intergenic (+48724/+4760)	unknown/unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (31/45);  total (31/45)

CGTTTACCTGCCGATTGATCCAATTCAAATCCAATTCCTGCGTTTGCACCAGTACTCGCCGCCAATATAAATCGCCAGGCGAACGCTCATCGCTCATAGCGATTTTATTTAACAGGGAAAAACACTTGTCAATATCCACCGCACTTTCATCGGCAATCAACTTCAAATACGCTTCAAGGTTTGTGGTCGGAACCAGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGCAGTTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCACAACAATAAGTGATAAAGCCAAGCTTCAATACGATCTTTATCTTTTACCGTCGCCATGCGCCAAGTGACGCGCTGTAGATTGTCACCGTATAAATGGGAAATGTTGCCCGACAACGTCATGTCACCCTCTTGGGTCGGCACGGTGA  >  1/1830380‑1830865                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                  cGTTTACCTGCCGATTGATCCAATTCAAATCCAATTCCTGCGTTTGCACCAGTACGCGCCGCCAATATAAATCGCCAGGCGAACGCTCATCGCCCATAGCGATTTTATTTAACAGGGAAAAACACTTGTCAACATCCACCGCACTTTCATCGGCAATCAGCTTCAAATACGCTTCAAGGTTTGTGGTCGGAATAAGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGTAGCTGGGCa                                                                                                                                                                                                                                             >  1:253975/1‑251 (MQ=255)     tgccTGCCGATTGGTCCAATTCAAATCCAATTCCTGCGTTTGCACCAGTACGCGCCGCCAATATAAATCGCCAGGCGAACGCTCATCGCCCATAGCGATTTTATTTAACAGGGAAAAACACTTGTCAACATCCACCGCACTTTCATCGGCAATCAGCTTCAAATACGCTTCAAGGTTTGTGGTCGGAATAAGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGTAGCTGGGCAACgg                                                                                                                                                                                                                                         <  1:413859/249‑1 (MQ=255)       ccTGCCGATTGATCCAATTCAAATCCAATTCCTGCGTTTGCACCAGTACGCGCCGCCAATATAAATCGCCAGGCGAACGCTCATCGCCCATAGCGATTTTATTTAACAGGGAAAAACACTTGTCAACATCCACCGCACTTTCATCGGCAATCAGCTTCAAATACGCTTCAAGGTTTGTGGTCGGAATAAGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCg                                                                                                                                                                                                                                       >  1:343079/1‑251 (MQ=255)                  aTCCAATTCAAATCCAATTCCTGCGTTTGCACCAGTACGCGCCGCCAATATAAATCGCCAGGCGAACGCTCATCGCCCATAGCGATTTTATTTAACAGGGAAAAACACTTGTCAACATCCACCGCACTTTCATCGGCAATCAGCTTCAAATACGCTTCAAGGTTTGTGGTCGGAATAAGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAgg                                                                                                                                                                                                                            <  1:316995/251‑1 (MQ=255)                   tCCAATTCAAATCCAATTCCTGCGTTTGCACCAGTACGCGCCGCCAATATAAATCGCCAGGCGAACGCTCATCGCCCATAGCGATTTTATTTAACAGGGAAAAACACTTGTCAACATCCACCGCACTTTCATCGGCAATCAGCTTCAAATACGCTTCAAGGTTTGTGGTCGGAATAAGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGc                                                                                                                                                                                                                           <  1:244997/251‑1 (MQ=255)                               ccAATTCCTGCGTTTGCACCAGTACGCGCCGCCAATATAAATCGCCAGGCGAACGCTCATCGCCCATAGCGATTTTATTTAACAGGGAAAAACACTTGTCAACATCCACCGCACTTTCATCGGCAATCAGCTTCAAATACGCTTCAAGGTTTGTGGTCGGAATAAGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAAc                                                                                                                                                                                                               <  1:348541/251‑1 (MQ=255)                                cAATTCCTGCGTTTGCACCAGTACGCGCCGCCAATATAAATCGCCAGGCGAACGCTCATCGCCCATAGCGATTTTATTTAACAGGGAAAAACACTTGTCAACATCCACCGCACTTTCATCGGCAATCAGCTTCAAATACGCTTCAAGGTTTGTGGTCGGAATAAGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAAcc                                                                                                                                                                                                              <  1:300787/251‑1 (MQ=255)                                  aTTCCTGCGTTTGCACCAGTACGCGCCGCCAATATAAATCGCCAGGCGAACGCTCATCGCCCATAGCGATTTTATTTAACAGGGAAAAACACTTGTCAACATCCACCGCACTTTCATCGGCAATCAGCTTCAAATACGCTTCAAGGTTTGTGGTCGGAATAAGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCg                                                                                                                                                                                                             >  1:189472/1‑250 (MQ=255)                                        gCGTTTGCACCAGTACGCGCCGCCAATATAAATCGCCAGGCGAACGCTCATCGCCCATAGCGATTTTATTTAACAGGGAAAAACACTTGTCAACATCCACCGCACTTTCATCGGCAATCAGCTTCAAATACGCTTCAAGGTTTGTGGTCGGAATAAGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCAcgcg                                                                                                                                                                                                       >  1:60331/1‑250 (MQ=255)                                            ttGCACCAGTACGCGCCGCCAATATAAATCGCCAGGCGAACGCTCATCGCCCATAGCGATTTTATTTAACAGGGAAAAACACTTGTCAACATCCACCGCACTTTCATCGGCAATCAGCTTCAAATACGCTTCAAGGTTTGTGGTCGGAATAAGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGc                                                                                                                                                                                                                           <  1:188966/226‑1 (MQ=255)                                                   aGTACGCGCCGCCAATATAAATCGCCAGGCGAACGCTCATCGCCCATAGCGATTTTATTTAACAGGGAAAAACACTTGTCAACATCCACCGCACTTTCATCGGCAATCAGCTTCAAATACGCTTCAAGGTTTGTGGTCGGAATAAGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTaa                                                                                                                                                                                                                 <  1:337895/229‑1 (MQ=255)                                                     tacgcgccgcCAATATAAATCGCCAGGCGAACGCTCATCGCCCATAGCGATTTTATTTAACAGGGAAAAACACTTGTCAACATCCACCGCACTTTCATCGGCAATCAGCTTCAAATACGCTTCAAGGTTTGTGGTCGGAATAAGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTcccc                                                                                                                                                                                          <  1:537249/246‑1 (MQ=255)                                                            cgccAATATAAATCGCCAGGCGAACGCTCATCGCCCATAGCGATTTTATTTAACAGGGAAAAACACTTGTCAACATCCACCGCACTTTCATCGGCAATCAGCTTCAAATACGCTTCAAGGTTTGTGGTCGGAATAAGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCGTcc                                                                                                                                                                                                                                    <  1:381131/201‑1 (MQ=255)                                                                                   aCGCTCATCGCCCATAGCGATTTTATTTAACAGGGAAAAACACTTGTCAACATCCACCGCACTTTCATCGGCAATCAGCTTCAAATACGCTTCAAGGTTTGTGGTCGGAATAAGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCg                                                                                                                                                           <  1:578729/251‑1 (MQ=255)                                                                                   aCGCTCATCGCCCATAGCGATTTTATTTAACAGGGAAAAACACTTGTCAACATCCACCGCACTTTCATCGGCAATCAGCTTCAAATACGCTTCAAGGTTTGTGGTCGGAATAAGCTGTAATTGCATCCGCCCCGCCAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCg                                                                                                                                                           <  1:187717/251‑1 (MQ=255)                                                                                      ctcatcgcccatAGCGATTTTATTTAACAGGGAAAAACACTTGTCAACATCCACCGCACTTTCATCGGCAATCAGCTTCAAATACGCTTCAAGGTTTGTGGTCGGAATAAGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGTAGCTGGGCAACgg                                                                                                                                                                                                                                         <  1:657476/170‑1 (MQ=255)                                                                                      ctcatcgcccatAGCGATTTTATTTAACAGGGAAAAACACTTGTCAACATCCACCGCACTTTCATCGGCAATCAGCTTCAAATACGCTTCAAGGTTTGTGGTCGGAATAAGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTgg                                                                                                                                                        >  1:389831/1‑251 (MQ=255)                                                                                            gcccatAGCGATTTTATTTAACAGGGAAAAACACTTGTCAACATCCACCGCACTTTCATCGGCAATCAGCTTCAAATACGCTTCAAGGTTTGTGGTCGGAATAAGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGcata                                                                                                                                                  >  1:315832/4‑249 (MQ=255)                                                                                             cccatAGCGATTTTATTTAACAGGGAAAAACACTTGTCAACATCCACCGCACTTTCATCGGCAATCAGCTTCAAATACGCTTCAAGGTTTGTGGTCGGAATAAGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGcataa                                                                                                                                                 >  1:483862/3‑248 (MQ=255)                                                                                                         ttatttaACAGGGAAAAACACTTGTCAACATCCACCGCACTTTCATCGGCAATCAGCTTCAAATACGCTTCAAGGTTTGTGGTCGGAATAAGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAAc                                                                                                                                                                                                               <  1:530002/177‑1 (MQ=255)                                                                                                         ttatttaACAGGGAAAAACACTTGTCAACATCCACCGCACTTTCATCGGCAATCAGCTTCAAATACGCTTCAAGGTTTGTGGTCGGAATAAGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATa                                                                                                                                     >  1:332441/1‑251 (MQ=255)                                                                                                         ttatttaACAGGGAAAAACACTTGTCAACATCCACCGCACTTTCATCGGCAATCAGCTTCAAATACGCTTCAAGGTTTGTGGTCGGAATAAGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATa                                                                                                                                     >  1:343017/1‑251 (MQ=255)                                                                                                              taACAGGGAAAAACACTTGTCAACATCCACCGCACTTTCATCGGCAATCAGCTTCAAATACGCTTCAAGGTTTGTGGTCGGAATAAGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGcc                                                                                                                                <  1:757376/251‑1 (MQ=255)                                                                                                                    ggAAAAACACTTGTCAACATCCACCGCACTTTCATCGGCAATCAGCTTCAAATACGCTTCAAGGTTTGTGGTCGGAATAAGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCaggc                                                                                                                            <  1:437705/249‑4 (MQ=255)                                                                                                                    ggAAAAACACTTGTCAACATCCACCGCACTTTCATCGGCAATCAGCTTCAAATACGCTTCAAGGTTTGTGGTCGGAATAAGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCt                                                                                                                           <  1:304416/250‑1 (MQ=255)                                                                                                                     gAAAAACACTTGTCAACATCCACCGCACTTTCATCGGCAATCAGCTTCAAATACGCTTCAAGGTTTGTGGTCGGAATAAGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTg                                                                                                                                        >  1:838763/1‑236 (MQ=255)                                                                                                                          acacTTGTCAACATCCACCGCACTTTCATCGGCAATCAGCTTCAAATACGCTTCAAGGTTTGTGGTCGGAATAAGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATAc                                                                                                                    <  1:328459/251‑1 (MQ=255)                                                                                                                          acacTTGTCAACATCCACCGCACTTTCATCGGCAATCAGCTTCAAATACGCTTCAAGGTTTGTGGTCGGAATAAGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATAc                                                                                                                    <  1:36619/251‑1 (MQ=255)                                                                                                                          acacTTGTCAACATCCACCGCACTTTCATCGGCAATCAGCTTCAAATACGCTTCAAGGTTTGTGGTCGGAATAAGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATAc                                                                                                                    <  1:394984/251‑1 (MQ=255)                                                                                                                               tGTCAACATCCACCGCACTTTCATCGGCAATCAGCTTCAAATACGCTTCAAGGTTTGTGGTCGGAATAAGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATACGatct                                                                                                               <  1:16359/251‑1 (MQ=255)                                                                                                                               tGTCAACATCCACCGCACTTTCATCGGCAATCAGCTTCAAATACGCTTCAAGGTTTGTGGTCGGAATAAGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATACGatct                                                                                                               <  1:227502/251‑1 (MQ=255)                                                                                                                               tGTCAACATCCACCGCACTTTCATCGGCAATCAGCTTCAAATACGCTTCAAGGTTTGTGGTCGGAATAAGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATACGat                                                                                                                 >  1:198765/1‑249 (MQ=255)                                                                                                                                gTCAACATCCACCGCACTTTCATCGGCAATCAGCTTCAAATACGCTTCAAGGTTTGTGGTCGGAATAAGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTg                                                                                                                                        >  1:865143/1‑225 (MQ=255)                                                                                                                                    acatCCACCGCACTTTCATCGGCAATCAGCTTCAAATACGCTTCAAGGTTTGTGGTCGGAATAAGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATACGatctttgtc                                                                                                          <  1:257595/249‑4 (MQ=255)                                                                                                                                       tCCACCGCACTTTCATCGGCAATCAGCTTCAAATACGCTTCAAGGTTTGTGGTCGGAATAAGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATACGatctttgtcttt                                                                                                       >  1:71343/1‑251 (MQ=255)                                                                                                                                          aCCGCACTTTCATCGGCAATCAGCTTCAAATACGCTTCAAGGTTTGTGGTCGGAATAAGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATACGATCTTTGTCTTTTAc                                                                                                    <  1:35369/251‑1 (MQ=255)                                                                                                                                                  ttCATCGGCAATCAGCTTCAAATACGCTTCAAGGTTTGTGGTCGGAATAAGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATACGatct                                                                                                               >  1:614990/1‑232 (MQ=255)                                                                                                                                                  ttCATCGGCAATCAGCTTCAAATACGCTTCAAGGTTTGTGGTCGGAATAAGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATACGATCTTTGTCTTTTACCGTCGCCa                                                                                            >  1:44270/1‑251 (MQ=255)                                                                                                                                                  ttCATCGGCAATCAGCTTCAAATACGCTTCAAGGTTTGTGGTCGGAATAAGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATACGATCTTTGTCTTTTACCGTCGCCa                                                                                            >  1:875909/1‑251 (MQ=255)                                                                                                                                                       cGGCAATCAGCTTCAAATACGCTTCAAGGTTTGTGGTCGGAATAAGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATACGATCTTTGTCTTTTACCGTCGCCATgcgc                                                                                       <  1:787968/251‑1 (MQ=255)                                                                                                                                                          cAATCAGCTTCAAATACGCTTCAAGGTTTGTGGTCGGAATAAGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATACGATCTTTGTCTTTTACCGTCGCCATGCGCCaa                                                                                    >  1:202204/1‑251 (MQ=255)                                                                                                                                                             tcagcTTCAAATACGCTTCAAGGTTTGTGGTCGGAATAAGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATACGATCTTTGTCTTTTACCGTCGCCATGCGCCAAGTg                                                                                 <  1:233613/247‑1 (MQ=255)                                                                                                                                                                gcTTCAAATACGCTTCAAGGTTTGTGGTCGGAATAAGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATACGATCTTTGTCTTTTACCGTCGCCATGCGCCAAGTGAcg                                                                              >  1:861918/2‑251 (MQ=255)                                                                                                                                                                gcTTCAAATACGCTTCAAGGTTTGTGGTCGGAATAAGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATACGATCTTTGTCTTTTACCGTCGCCATGCGCCAAGTGAcg                                                                              >  1:365544/2‑251 (MQ=255)                                                                                                                                                                gcTTCAAATACGCTTCAAGGTTTGTGGTCGGAATAAGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATACGATCTTTGTCTTTTACCGTCGCCATGCGCCAAGTGAcg                                                                              >  1:516477/2‑251 (MQ=255)                                                                                                                                                                            cTTCAAGGTTTGTGGTCGGAATAAGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATACGATCTTTGTCTTTTACCGTCGCCATGCGCCAAGTGACGCGCTGTAGATTg                                                                  >  1:856207/1‑251 (MQ=255)                                                                                                                                                                            cTTCAAGGTTTGTGGTCGGAATAAGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGGTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGGCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATACGATCTTTGTCTTTTACCGTCGCCATGCGCCAAGTGACGCGCTGTAGAtt                                                                   <  1:414920/250‑1 (MQ=255)                                                                                                                                                                              tCAAGGTTTGTGGTCGGAATAAGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATACGATCTTTGTCTTTTACCGTCGCCATGCGCCAAGTGACGCGCTGTAGATTGTc                                                                <  1:857408/251‑1 (MQ=255)                                                                                                                                                                              tCAAGGTTTGTGGTCGGAATAAGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATACGATCTTTGTCTTTTACCGTCGCCATGCGCCAAGTGACGCGCTGTAGATTGTc                                                                <  1:788160/251‑1 (MQ=255)                                                                                                                                                                              tCAAGGTTTGTGGTCGGAATAAGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATACGATCTTTGTCTTTTACCGTCGCCATGCGCCAAGTGACGCGCTGTAGATTGTc                                                                <  1:692905/251‑1 (MQ=255)                                                                                                                                                                              tCAAGGTTTGTGGTCGGAATAAGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATACGATCTTTGTCTTTTACCGTCGCCATGCGCCAAGTGACGCGCTGTAGATTGTc                                                                <  1:354445/251‑1 (MQ=255)                                                                                                                                                                               cAAGGTTTGTGGTCGGAATAAGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGa                                                                                                                                                                               >  1:226182/1‑139 (MQ=255)                                                                                                                                                                                  ggTTTGTGGTCGGAATAAGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATACGATCTTTGTCTTTTACCg                                                                                                  >  1:844337/1‑213 (MQ=255)                                                                                                                                                                                  ggTTTGTGGTCGGAATAAGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATACGATCTTTGTCTTTTACCGTCGCCATGCGCCAAGt                                                                                  >  1:297581/1‑229 (MQ=255)                                                                                                                                                                                          gTCGGAATAAGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATACGATCTTTGTCTTTTACCGTCGCCATGCGCCAAGTGACGCGCTGTAGATTGTCACCGTATAAAt                                                     <  1:692636/250‑1 (MQ=255)                                                                                                                                                                                              gaataaGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACCTTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATACGATCTTTGTCTTTTACCGTCGCCATGCGCCAAGTGACGCGCTCTAGATTGTCACCGTATAAATAGGat                                                >  1:152824/6‑250 (MQ=255)                                                                                                                                                                                              gaataaGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTgg                                                                                                                                                                                       <  1:175832/111‑1 (MQ=255)                                                                                                                                                                                              gaataaGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATACGATCTTTGTCTTTTACCGTCGCCATGCGCCAAGTGACGCGCTGTAGa                                                                     <  1:285260/225‑1 (MQ=255)                                                                                                                                                                                              gaataaGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATACGATCTTTGTCTTTTACCGTCGCCATGCGCCAAGTGACGCGCTGTAGa                                                                     <  1:386428/225‑1 (MQ=255)                                                                                                                                                                                              gaataaGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATACGATCTTTGTCTTTTACCGTCGCCATGCGCCAAGTGACGCGCTGTAGATTGTCACCGTATAAATAGGat                                                >  1:493806/6‑250 (MQ=255)                                                                                                                                                                                                  aaGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATACGATCTTTGTCTTTTACCGTCGCCATGCGCCAAGTGACGCGCTGTAGATTGTCACCGTATAAATAGGATATGt                                            <  1:586465/250‑1 (MQ=255)                                                                                                                                                                                                     cTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGaaa                                                                                                                                                                                    >  1:824787/1‑112 (MQ=255)                                                                                                                                                                                                     cTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATACGATCTTTGTCTTTTACCGTCGCCATGCGCCAAGTGACGCGCTGTAGATTGTCACCGTATAAATAGGATATGt                                            <  1:851581/248‑1 (MQ=255)                                                                                                                                                                                                       gTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATACGatc                                                                                                                <  1:245305/178‑1 (MQ=255)                                                                                                                                                                                                         aaTTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATACGATCTTTGTCTTTTACCGTCGCCATGCGCCAAGTGACGCGCTGTAGATTGTCACCGTATAAATAGGATATg                                             >  1:180634/1‑243 (MQ=255)                                                                                                                                                                                                                         ccAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATACGatctttgtct                                                                                                         <  1:635883/167‑1 (MQ=255)                                                                                                                                                                                                                         ccAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATACGat                                                                                                                 <  1:69528/159‑1 (MQ=255)                                                                                                                                                                                                                         ccAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATACGATCTTTGTCTTTTACCGTCGCCATGCGCCAAGTGACGCGCTGTAGATTGTCACCGTATAAAt                                                     <  1:832427/219‑1 (MQ=255)                                                                                                                                                                                                                                aCTTTCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATACGATCTTTGTCTTTTACCGTCGCCATGCGCCAAGTGACGCGCTGTAGATTGTCACCGTTTAACTAGGATATGTTGCCCGACAACGTCATGTCACCCTCTTggg              <  1:430409/251‑1 (MQ=255)                                                                                                                                                                                                                                  tttCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATACGATCTTTGTCTTTTACCGTCGCCATGCGCCAAGTGACGCGCTGTAGATTGTCACCGTATAAATAGGATATGTTGCCCGACAACGTCATGTCACCCTCTTGGGTc            <  1:534107/251‑1 (MQ=255)                                                                                                                                                                                                                                       acaTAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATACGATCTTTGTCTTTTACCGTCGCCATGCGCCAAGTGACGCGCTGTAGATTGTCACCGTATAAATAGGATATGTTGCCCGACAACGTCATGTCACCCTCTTGGGTCAGCAc       <  1:708341/251‑1 (MQ=255)                                                                                                                                                                                                                                       acaTAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATACGATCTTTGTCTTTTACCGTCGCCATGCGCCAAGTGACGCGCTGTAGATTGTCACCGTATAAATAGGATATGTTGCCCGACAACGTCATGTCACCCTCTTGGGTCAGCAc       <  1:234999/251‑1 (MQ=255)                                                                                                                                                                                                                                            aaTTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATACGATCTTTGTCTTTTACCGTCGCCATGCGCCAAGTGACGCGCTGTAGATTGTCACCGTATAAAtag                                                   >  1:669037/1‑200 (MQ=255)                                                                                                                                                                                                                                            aaTTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATACGATCTTTGTCTTTTACCGTCGCCATGCGCCAAGTGACGCGCTGTAGATTGTCACCGTATAAATAGGATATGTTGCCCGACAACGTCATGTCACCCTCTTGGGTCAGCACGGTGa  >  1:651069/1‑251 (MQ=255)                                                                                                                                                                                                                                             aTTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGgcgc                                                                                                                                                     <  1:886429/103‑1 (MQ=255)                                                                                                                                                                                                                                             aTTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCATAACAATAAGTGATAAAGCCAGGCTTCAATACGATCTTTGTCTTTTACCGTCGCCATGCGCCAAGTGACGCGCTGTAGATTGTCACCGTATAAATAGGATATGTTGCCCGACAACGTCATGTCACCCTCTTGGGTc            >  1:601763/1‑240 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                  CGTTTACCTGCCGATTGATCCAATTCAAATCCAATTCCTGCGTTTGCACCAGTACTCGCCGCCAATATAAATCGCCAGGCGAACGCTCATCGCTCATAGCGATTTTATTTAACAGGGAAAAACACTTGTCAATATCCACCGCACTTTCATCGGCAATCAACTTCAAATACGCTTCAAGGTTTGTGGTCGGAACCAGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGCAGTTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCGCTGATCTTTCCCCTGGAAAAGCGATTCCGTCGGCTGTTCCTGCGTGGCGCACAACAATAAGTGATAAAGCCAAGCTTCAATACGATCTTTATCTTTTACCGTCGCCATGCGCCAAGTGACGCGCTGTAGATTGTCACCGTATAAATGGGAAATGTTGCCCGACAACGTCATGTCACCCTCTTGGGTCGGCACGGTGA  >  1/1830380‑1830865

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑