Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,830,435	T→G	intergenic (+48535/+4949)	unknown → / ← unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,830,435	0	T	G	100.0%	171.5 / NA	60	intergenic (+48535/+4949)	unknown/unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (30/30);  total (30/30)

TTGTGGCTGGAAGTGCGCGAATCCAATAAAAAAGCCCAAGCGCTGTATGATTCGCTGGGCTTTAATCAGGTGGATATTCGCAAAAATTATTATCCCACCCCCGACGGCAAACGGGAAAATGCCGTGGTGATGGCGGCGTATTTATAGGCTATTTTTCCCTTTTGATAGCTTGCAACATCGGCGCAAACCAAGCGTTTACCTGCCGATTGATCCAATTCAAATCCAATTCCTGCGTTTGCACCAGTACTCGCCGCCAATATAAATCGCCAGGCGAACGCTCATCGCTCATAGCGATTTTATTTAACAGGGAAAAACACTTGTCAATATCCACCGCACTTTCATCGGCAATCAACTTCAAATACGCTTCAAGGTTTGTGGTCGGAACCAGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGCAGTTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCG  >  1/1830188‑1830668                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                          ttGTGGCTGGAAGTGCGCGAATCCAATAAAAAAGCCCTGGCACTGTATGATTCGCTGGGCTTTAATCAAGTGGATATTCGCAAAAATTATTATCCCACCGCCGACGGCAAACGGGAAAATGCCGTGGTGATGGCGGCGTATTTATAGGCTATTTTTCCCTTTTGATAGCTTGCAACATCGGTGCAAACCAAGCGTTTACCTGCCGATTGATCCAATTCAAATCCAATTCCTGCGTTTGCACCAGTACgcgc                                                                                                                                                                                                                                        <  1:193862/251‑1 (MQ=255)     ggCTGGAAGTGCGCGAATCCAATAAAAAAGCCCTGGCACTGTATGATTCGCTGGGCTTTAATCAAGTGGATATTCGCAAAAATTATTATCCCACCGCCGACGGCAAACGGGAAAATGCCGTGGTGATGGCGGCGTATTTATAGGCTATTTTTCCCTTTTGATAGCTTGCAACATCGGTGCAAACCAAGCGTTTACCTGCCGATTGATCCAATTCAAATCCAATTCCTGCGTTTGCACCAGTACGcgccgc                                                                                                                                                                                                                                     >  1:101193/1‑250 (MQ=255)           aaGTGCGCGAATCCAATAAAAAAGCCCTGGCACTGTATGATTCGCTGGGCTTTAATCAAGTGGATATTCGCAAAAATTATTATCCCACCGCCGACGGCAAACGGGAAAATGCCGTGGTGATGGCGGCGTATTTATAGGCTATTTTTCCCTTTTGATAGCTTGCAACATCGGTGCAAACCAAGCGTTTACCTGCCGATTGATCCAATTCAAATCCAATTCCTGCGTTTGCACCAGTACGCGCCGCCAatat                                                                                                                                                                                                                               <  1:88511/250‑1 (MQ=255)                            aaaaaaGCCCTGGCACTGTATGATTCGCTGGGCTTTAATCAAGTGGATATTCGCAAAAATTATTATCCCACCGCCGACGGCAAACGGGAAAATGCCGTGGTGATGGCGGCGTATTTATAGGCTATTTTTCCCTTTTGATAGCTTGCAACATCGGTGCAAACCAAGCGTTTACCTGCCGATTGATCCAATTCAAATCCAATTCCTGCGTTTGCACCAGTACGCGCCGCCAATATAAATCGCCAGGCGAAcg                                                                                                                                                                                                              >  1:56672/1‑250 (MQ=255)                                  gccctggcacTGTATGATTCGCTGGGCTTTAATCAAGTGGATATTCGCAAAAATTATTATCCCACCGCCGACGGCGAACGGGAAAATGCCGTGGTGATGGCGGCGTATTTATAGGCTATTTTTCCCTTTTGATAGCTTGCAACATCGGTGCAAACCAAGCGTTTACCTGCCGATTGATCCAATTCAAATCCAATTCCTGCGTTTGCACCAGTACGCGCCGCCAATATAAATCGCCAGGCGAAcgctcatcg                                                                                                                                                                                                       >  1:702989/10‑251 (MQ=255)                                   ccctggcacTGTATGATTCGCTGGGCTTTAATCAAGTGGATATTCGCAAAAATTATTATCCCACCGCCGACGGCAAACGGGAAAATGCCGTGGTGATGGCGGCGTATTTATAGGCTATTTTTCCCTTTTGATAGCTTGCAACATCGGTGCAAACCAAGCGTTTACCTGCCGATTGATCCAATTCAAATCCAATTCCTGCGTTTGCACCAGTACGCGCCGCCAATATAAATc                                                                                                                                                                                                                          <  1:533480/223‑1 (MQ=255)                                          acTGTATGATTCGCTGGGCTTTAATCAAGGGGATATTCGCAAAAATTATTATCCCACCGCCGACGGCAAACGGGAAAATGCCGTGGTGATGGCGGCGTATTTATAGGCTATTTTTCCCTTTTGATAGCTTGCAACATCGGTGCAAACCAAGCGTTTACCTGCCGATTGATCCAATTCAAATCCAATTCCTGCGTTTGCACCAGTACGCGCCGCCAATATAAATCGCCAGGCGAACGCTCATCGCCCATAGc                                                                                                                                                                                               <  1:732879/250‑1 (MQ=255)                                           cTGTATGATTCGCTGGGCTTTAATCAAGTGGATATTCGCAAAAATTATTATCCCTCCGCCGACGGCAAACGGGAAAATGCCGTGGTGATGGCGGCGTATTTATAGGCTATTTTTCCCTTTTGATAGCTTGCAACATCGGTGCAAACCAAGCGTTTACCTGCCGATTGATCCAATTCAAATCCAATTCCTGCGTTTGCACCAGTACGCGCCGCCAATATAAATCGCCAGGCGAACGCTCATCGCCCATAGCg                                                                                                                                                                                              <  1:44199/251‑1 (MQ=255)                                           cTGTATGATTCGCTGGGCTTTAATCAAGTGGATATTCGCAAAAATTATTATCCCACCGCCGACGGCAAACGGGAAAATGCCGTGGTGATGGCGGCGTATTTATAGGCTATTTTTCCCTTTTGATAGCTTGCAACATCGGTGCAAACCAAGCGTTTACCTGCCGATTGATCCAATTCAAATCCAATTCCTGCGTTTGCACCAGTACGCGCCGCCAatat                                                                                                                                                                                                                               <  1:645302/218‑1 (MQ=255)                                           cTGTATGATTCGCTGGGCTTTAATCAAGTGGATATTCGCAAAAATTATTATCCCACCGCCGACGGCAAACGGGAAAATGCCGTGGTGATGGCGGCGTATTTATAGGCTATTTTTCCCTTTTGATAGCTTGCAACATCGGTGCAAACCAAGCGTTTACCTGCCGATTGATCCAATTCAAATCCAATTCCTGCGTTTGCACCAGTACGCGCCGCCAATATAAATCGCCAGGCGAACGCTCATCGCCCATAGCg                                                                                                                                                                                              <  1:87922/251‑1 (MQ=255)                                             gTATGATTCGCTGGGCTTTAATCAAGTGGATATTCGCAAAAATTATTATCCCACCGCCGACGGCAAACGGGAAAATGCCGTGGTGATGGCGGCGTATTTATAGGCTATTTTTCCCTTTTGATAGCTTGCAACATCGGTGCAAACCAAGCGTTTACCTGCCGATTGATCCAATTCAAATCCAATTCCTGCGTTTGCACCAGTACGCGCCGCCAATATAAATCGCCAGGCGAACGCTCATCGCCCATAGCGAt                                                                                                                                                                                            >  1:303973/1‑251 (MQ=255)                                             gTATGATTCGCTGGGCTTTAATCAAGTGGATATTCGCAAAAATTATTATCCCACCGCCGACGGCAAACGGGAAAATGCCGTGGTGATGGCGGCGTATTTATAGGCTATTTTTCCCTTTTGATAGCTTGCAACATCGGTGCAAACCAAGCGTTTACCTGCCGATTGATCCAATTCAAATCCAATTCCTGCGTTTGCACCAGTACGCGCCGCCAATATAAATCGCCAGGCGAACGCTCATCGCCCATAGCGAt                                                                                                                                                                                            >  1:299026/1‑251 (MQ=255)                                                            cTTTAATCAAGTGGATATTCGCAAAAATTATTATCCCACCGCCGACGGCAAACGGGAAAATGCCGTGGTGATGGCGGCGTATTTATAGGCTATTTTTCCCTTTTGATAGCTTGCAACATCGGTGCAAACCAAGCGTTTACCTGCCGATTGATCCAATTCAAATCCAATTCCTGCGTTTGCACCAGTACGCGCCGCCAATATAAATCGCCAGGCGAACGCTCATCGCCCATAGCGATTTTATTTAACAGGGa                                                                                                                                                                             <  1:518190/251‑1 (MQ=255)                                                                  tcaagTGGATATTCGCAAAAATTATTATCCCACCGCCGACGGCAAACGGGAAAATGCCGTGGTGATGGCGGCGTATTTATAGGCTATTTTTCCCTTTTGATAGCTTGCAACATCGGTGCAAACCAAGCGTTTACCTGCCGATTGATCCAATTCAAATCCAATTCCTGCGTTTGCACCAGTACGCGCCGCCAATATAAATCGCCAGGCGAACGCTCATCGCCCATAGCGATTTTATTTAACAGGGAAAAaca                                                                                                                                                                       <  1:207130/247‑1 (MQ=255)                                                                   caagTGGATATTCGCAAAAATTATTATCCCACCGCCGACGGCAAACGGGAAAATGCCGTGGTGATGGCGGCGTATTTATAGGCTATTTTTCCCTTTTGATAGCTTGCAACATCGGTGCAAACCAAGCGTTTACCTGCCGATTGATCCAATTCAAATCCAATTCCTGCGTTTGCACCAGTACGCGCCGCCAATATAAATCGCCAGGCGAACGCTCATCGCCCATAGCGATTTTATTTAACAGGGAAAAacac                                                                                                                                                                      <  1:395192/248‑1 (MQ=255)                                                                                      attattATCCCACCGCCGACGGCAAACGGGAAAATGCCGTGGTGATGGCGGCGTATTTATAGGCTATTTTTCCCTTTTGATAGCTTGCAACATCGGTGCAAACCAAGCGTTTACCTGCCGATTGATCCAATTCAAATCCAATTCCTGCGTTTGCACCAGTACGCGCCGCCAATATAAATCGCCAGGCGAACGCTCATCGCCCATAGCGATTTTATTTAACAg                                                                                                                                                                                >  1:778701/1‑222 (MQ=255)                                                                                       ttattaTCCCACCGCCGACGGCAAACGGGAAAATGCCGTGGTGATGGCGGCGTATTTATAGGCTATTTTTCCCTTTTGATAGCTTGCAACATCGGTGCAAACCAAGCGTTTACCTGCCGATTGATCCAATTCAAATCCAATTCCTGCGTTTGCACCAGTACGCGCCGCCAATATAAATCGCCAGGCGAACGCTCATCGCCCATAGCGATTTTATTTAACAGGGAAAAACACTTGTCAACATCCACCGCa                                                                                                                                                    >  1:315115/1‑249 (MQ=255)                                                                                       ttattaTCCCACCGCCGACGGCAAACGGGAAAATGCCGTGGTGATGGCGGCGTATTTATAGGCTATTTTTCCCTTTTGATAGCTTGCAACATCGGTGCAAACCAAGCGTTTACCTGCCGATTGATCCAATTCAAATCCAATTCCTGCGTTTGCACCAGTACGCGCCGCCAATATAAATCGCCAGGCGAACGCTCATCGCCCATAGCGATTTTATTTAACAGGGAAAAACACTTGTCAACATCCACCGCa                                                                                                                                                    >  1:555398/1‑249 (MQ=255)                                                                                           tatCCCACCGCCGACGGCAAACGGGAAAATGCCGTGGTGATGGCGGCGTATTTATAGGCTATTTTTCCCTTTTGATAGCTTGCAACATCGGTGCAAACCAAGCGTTTACCTGCCGATTGATCCAATTCAAATCCAATTCCTGCGTTTGCACCAGTACGCGCCGCCAATATAAATCGCCAGGCGAACGCTCATCGCCCATAGCGATTTTATTTAACAGGGAAAAACACTTGTCAACATCCACCGCACTTTCa                                                                                                                                              <  1:846844/251‑1 (MQ=255)                                                                                            atCCCACCGCCGACGGCAAACGGGAAAATGCCGTGGTGATGGCGGCGTATTTATAGGCTATTTTTCCCTTTTGATAGCTTGCAACATCGGTGCAAACCAAGCGTTTACCTGCCGATTGATCCAATTCAAATCCAATTCCTGCGTTTGCACCAGTACGCGCCGCCAATATAAATCGCCAGGCGAACGCTCATCGCCCATAGCGATTTTATTTAACAGGGAAAAACACTTGTCAACATCCACCGCACTTTCAt                                                                                                                                             <  1:840702/251‑1 (MQ=255)                                                                                                         cGGCAAACGGGAAAATGCCGTGGTGATGGCGGCGTATTTATAGGCTATTTTTCCCTTTTGATAGCTTGCAACATCGGTGCAAACCAAGCGTTTACCTGCCGATTGATCCAATTCAAATCCAATTCCTGCGTTTGCACCAGTACGCGCCGCCAATATAAATCGCCAGGCGAACGCTCATCGCCCATAGCGATTTTATTTAACAGGGAAAAACACTTGTCAACATCCACCGCACTTTCATCGGCAATCAGCtt                                                                                                                                >  1:465869/1‑251 (MQ=255)                                                                                                              aaCGGGAAAATGCCGTGGTGATGGCGGCGTATTTATAGGCTATTTTTCCCTTTTGATAGCTTGCAACATCGGTGCAAACCAAGCGTTTACCTGCCGATTGATCCAATTCAAATCCAATTCCTGCGTTTGCACCAGTACGCGCCGCCAATATAAATCGCCAGGCGAACGCTCATCGCCCATAGCGATTTTATTTAACAGGGAAAAACACTTGTCAACATCCACCGCACTTTCATCGGCAATCAGCTTCAAAt                                                                                                                           <  1:779334/251‑1 (MQ=255)                                                                                                                cGGGAAAATGCCGTGGTGATGGCGGCGTATTTATAGGCTATTTTTCCCTTTTGATAGCTTGCAACATCGGTGCAAACCAAGCGTTTACCTGCCGATTGATCCAATTCAAATCCAATTCCTGCGTTTGCACCAGTACGCGCCGCCAATATAAATCGCCAGGCGAACGCTCATCGCCCATAGCGATTTTATTTAACAGGGAAAAACACTTGTCAACATCCACCGCACTTTCa                                                                                                                                              <  1:474760/230‑1 (MQ=255)                                                                                                                 gggAAAATGCCGTGGTGATGGCGGCGTATTTATAGGCTATTTTTCCCTTTTGATAGCTTGCAACATCGGTGCAAACCAAGCGTTTACCTGCCGATTGATCCAATTCAAATCCAATTCCTGCGTTTGCACCAGTACGCGCCGCCAATATAAATCGc                                                                                                                                                                                                                        >  1:45849/1‑155 (MQ=255)                                                                                                                  ggAAAATGCCGTGGTGATGGCGGCGTATTTATAGGCTATTTTTCCCTTTTGATAGCTTGCAACATCGGTGCAAACCAAGCGTTTACCTGCCGATTGATCCAATTCAAATCCAATTCCTGCGTTTGCACCAGTACGCGCCGCCAATATAAATCGCCAGGCGAACGCTCATCGCCCATAGCGATTTTATTTAACAGGGAAAAACACTTGTCAACATCCACCGCACTTTCATCGGCAATCAGCTTCAAATACg                                                                                                                        >  1:324014/1‑250 (MQ=255)                                                                                                                  ggAAAATGCCGTGGTGATGGCGGCGTATTTATAGGCTATTTTTCCCTTTTGATAGCTTGCAACATCGGTGCAAACCAAGCGTTTACCTGCCGATTGATCCAATTCAAATCCAATTCCTGCGTTTGCACCAGTACGCGCCGCCAATATAAATCGCCAGGCGAACGCTCATCGCCCATAGCGATTTTATTTAACAGGGAAAAACACTTGTCAACATCCACCGCACTTTCATCGGCAATCAGCTTCAAATACg                                                                                                                        >  1:638120/1‑250 (MQ=255)                                                                                                                   gAAAATGCCGTGGTGATGGCGGCGTATTTATAGGCTATTTTTCCCTTTTGATAGCTTGCAACATCGGTGCAAACCAAGCGTTTACCTGCCGATTGATCCAATTCAAATCCAATTCCTGCGTTTGCACCAGTACGCGCCGCCAATATAAATCGCCAGGCGAACGCTCATCGCCCATAGCGATTTTATTTAACAGGGAAAAACACTTGTCAACATCCACCGCACTTTCATCGGCAATCAGCTTCAAATACGc                                                                                                                       <  1:567747/250‑1 (MQ=255)                                                                                                                              ggtgATGGCGGCGTATTTATAGGCTATTTTTCCCTTTTGATAGCTTGCAACATCGGTGCAAACCAAGCGTTTACCTGCCGATTGATCCAATTCAAATCCAATTCCTGCGTTTGCACCAGTACGCGCCGCCAATATAAATCGCCAGGCGAACGCTCATCGCCCATAGCGATTTTATTTAACAGGGAAAAACACTTGTCAACATCCACCGCACTTTCATCGGCAATCAGCTTCAAATACGCTTCAAgg                                                                                                                <  1:457351/246‑1 (MQ=255)                                                                                                                                            aTTTATAGGCTATTTTTCCCTTTTGATAGCTTGCAACATCGGTGCAAACCAAGCGTTTACCTGCCGATTGATCCAATTCAAATCCAATTCCTGCGTTTGCACCAGTACGCGCCGCCAATATAAATCGCCAGGCGAACGCTCATCGCCCATAGCGATTTTATTTAACAGGGAAAAACACTTGTCAACATCCACCGCACTTTCATCGGCAATCAGCTTCAAATACGc                                                                                                                       >  1:224257/1‑225 (MQ=255)                                                                                                                                            aTTTATAGGCTATTTTTCCCTTTTGATAGCTTGCAACATCGGTGCAAACCAAGCGTTTACCTGCCGATTGATCCAATTCAAATCCAATTCCTGCGTTTGCACCAGTACGCGCCGCCAATATAAATCGCCAGGCGAACGCTCATCGCCCATAGCGATTTTATTTAACAGGGAAAAACACTTGTCAACATCCACCGCACTTTCATCGGCAATCAGCTTCAAATACGCTTCAAGGTTTGTGGTCGGaataag                                                                                               >  1:302393/1‑245 (MQ=255)                                                                                                                                             tttATAGGCTATTTTTCCCTTTTGATAGCTTGCAACATCGGTGCAAACCAAGCGTTTACCTGCCGATTGATCCAATTCAAATCCAATTCCTGCGTTTGCACCAGTACGCgccgcc                                                                                                                                                                                                                                    <  1:183528/115‑1 (MQ=255)                                                                                                                                             tttATAGGCTATTTTTCCCTTTTGATAGCTTGCAACATCGGTGCAAACCAAGCGTTTACCTGCCGATTGATCCAATTCAAATCCAATTCCTGCGTTTGCACCAGTACGCGCCGCCAATATAAATCGCCAGGCGAACGCTCATCGCCCATAGCGATTTTATTTAACAGGGAAAAACACTTGTCAACATCCACCGCACTTTCATCGGCAATCAGCTTCAAATACGCTTCAAGGTTTGTGGTCGGaataag                                                                                               >  1:272718/1‑244 (MQ=255)                                                                                                                                             tttATAGGCTATTTTTCCCTTTTGATAGCTTGCAACATCGGTGCAAACCAAGCGTTTACCTGCCGATTGATCCAATTCAAATCCAATTCCTGCGTTTGCACCAGTACGCGCCGCCAATATAAATCGCCAGGCGAACGCTCATCGCCCATAGCGATTTTATTTAACAGGGAAAAACACTTGTCAACATCCACCGCACTTTCATCGGCAATCAGCTTCAAATACGCTTCAAGGTTTGTGGTCGGaataag                                                                                               >  1:366794/1‑244 (MQ=255)                                                                                                                                                       aTTTTTCCCTTTTGATAGCTTGCAACATCGGTGCAAACCAAGCGTTTACCTGCCGATTGATCCAATTCAAATCCAATTCCTGCGTTTGCACCAGTACGCGCCGCCAATATAAATCGCCAGGCGAACGCTCATCGCCCATAGCGATTTTATTTAACAGGGAAAAACACTTGTCAACATCCACCGCACTTTCATCGGCAATCAGCTTCAAATACGCTTCAAGGTTTGTGGTCGGAATAAGCTGTAATTg                                                                                      >  1:341605/1‑247 (MQ=255)                                                                                                                                                       aTTTTTCCCTTTTGATAGCTTGCAACATCGGTGCAAACCAAGCGTTTACCTGCCGATTGATCCAATTCAAATCCAATTCCTGCGTTTGCACCAGTACGCGCCGCCAATATAAATCGCCAGGCGAACGCTCATCGCCCATAGCGATTTTATTTAACAGGGAAAAACACTTGTCAACATCCACCGCACTTTCATCGGCAATCAGCTTCAAATACGCTTCAAGGTTTGTGGTCGGAATAAGCTGTAATTGCg                                                                                    >  1:97403/1‑249 (MQ=255)                                                                                                                                                                 tttGATAGCTTGCAACATCGGTGCAAACCAAGCGTTTACCTGCCGATTGATCCAATTCAAATCCAATTCCTGCGTTTGCACCAGTACGCGCCGCCAATATAAATCGCCAGGCGAACGCTCATCGCCCATAGCGATTTTATTTAACAGGGAAAAACACTTGTCAACATCCACCGCACTTTCATCGGCAATCAGCTTCAAATACGCTTCAAGGTTTGTGGTCGGaata                                                                                                 >  1:479562/1‑224 (MQ=255)                                                                                                                                                                  ttGATAGCTTGCAACATCGGTGCAAACCAAGCGTTTACCTGCCGATTGATCCAATTCAAATCCAATTCCTGCGTTTGCACCAGTACGCGCCGCCAATATAAATCGCCAGGCGAACGCTCATCGCCCATAGCGATTTTATTTAACAGGGAAAAACACTTGTCAACATCCACCGCACTTTCATCGGCAATCAGCTTCAAATACGCTTCAAGGTTTGTGGTCGGAATAAGCTGTAATTGCGTCCGCCCCGCCaa                                                                       >  1:87500/1‑251 (MQ=255)                                                                                                                                                                        gCTTGCAACATCGGTGCAAACCAAGCGTTTACCTGCCGATTGATCCAATTCAAATCCAATTCCTGCGTTTGCACCAGTACGCGCCGCCAATATAAATCGCCAGGCGAACGCTCATCGCCCATAGCGATTTTATTTAACAGGGAAAAACACTTGTCAACATCCACCGCACTTTCATCGGCAATCAGCTTCAAATACGCTTCAAGGTTTGTGGTCGGAATAAGCTGTAATTGCGTCCGCCCCGCCAAATaa                                                                   >  1:76695/1‑249 (MQ=255)                                                                                                                                                                         cTTGCAACATCGGTGCAAACCAAGCGTTTACCTGCCGATTGATCCAATTCAAATCCAATTCCTGCGTTTGCACCAGTACGCGCCGCCAATATAAATCGCCAGGCGAACGCTCATCGCCCATAGCGATTTTATTTAACAGGGAAAAACACTTGTCAACATCCACCGCACTTTCATCGGCAATCAGCTTCAAATACGCTTCAAGGTTTGTGGTCGGAATAAGCTGTAATTGCGTCCGCCCCGCCAAATAACtt                                                                <  1:26440/251‑1 (MQ=255)                                                                                                                                                                         cTTGCAACATCGGTGCAAACCAAGCGTTTACCTGCCGATTGATCCAATTCAAATCCAATTCCTGCGTTTGCACCAGTACGCGCCGCCAATATAAATCGCCAGGCGAACGCTCATCGCCCATAGCGATTTTATTTAACAGGGAAAAACACTTGTCAACATCCACCGCACTTTCATCGGCAATCAGCTTCAAATACGCTTCAAGGTTTGTGGTCGGAATAAGCTGTAATTGCGTCCGCCCCGCCAAATAACtt                                                                <  1:652126/251‑1 (MQ=255)                                                                                                                                                                          ttGCAACATCGGTGCAAACCAAGCGTTTACCTGCCGATTGATCCAATTCAAATCCAATTCCTGCGTTTGCACCAGTACGCGCCGCCAATATAAATCGCCAGGCGAACGCTCATCGCCCATAGCGATtttattt                                                                                                                                                                                     <  1:610971/133‑1 (MQ=255)                                                                                                                                                                               aCATCGGTGCAAACCAAGCGTTTACCTGCCGATTGATCCAATTCAAATCCAATTCCTGCGTTTGCACCAGTACGCGCCGCCAATATAAATCGCCAGGCGAACGCTCATCGCCCATAGCGATTTTATTTAACAGGGAAAAACACTTGTCAACATCCACCGCACTTTCATCGGCAATCAGCTTCAAATACGCTTCAAGGTTTGTGGTCGGAATAAGCTGTAAt                                                                                        >  1:309346/1‑221 (MQ=255)                                                                                                                                                                                cATCGGTGCAAACCAAGCGTTTACCTGCCGATTGATCCAATTCAAATCCAATTCCTGCGTTTGCACCAGTACGCGCCGCCAATATAAATCGCCAGGCGAACGCTCATCGCCCATAGCGATTTTATTTAACAGGGAAAAACACTTGTCaac                                                                                                                                                              <  1:763101/150‑2 (MQ=255)                                                                                                                                                                                     gtgcAAACCAAGCGTTTACCTGCCGATTGATCCAATTCAAATCCAATTCCTGCGTTTGCACCAGTACGCGCCGCCAATATAAATCGCCAGGCGAACGCTCATCGCCCATAGCGATTTTATTTAACAGGGAAAAACACTTGTCAACATCCACCGCACTTTCATCGGCAATCAGCTTCCAATACGCTTCAAGGTTTGTGGTCGGAATAAGCTGTAATTg                                                                                      >  1:109147/3‑217 (MQ=255)                                                                                                                                                                                     gtgcAAACCAAGCGTTTACCTGCCGATTGATCCAATTCAAATCCAATTCCTGCGTTTGCACCAGTACGCGCCGCCAATATAAATCGCCAGGCGAACGCTCATCGCCCATAGCGATTTTATTTAACAGGGAAAAACACTTGTCAACATCCACCGCACTTTCATCGGCAATCAGCTTCAAATACGCTTCAAGGTTTGTGGTCGGaataagctg                                                                                            >  1:269/3‑204 (MQ=255)                                                                                                                                                                                     gtgcAAACCAAGCGTTTACCTGCCGATTGATCCAATTCAAATCCAATTCCTGCGTTTGCACCAGTACGCGCCGCCAATATAAATCGCCAGGCGAACGCTCATCGCCCATAGCGATTTTATTTAACAGGGAAAAACACTTGTCAACATCCACCGCACTTTCATCGGCAATCAGCTTCAAATACGCTTCAAGGTTTGTGGTCGGaataagc                                                                                              <  1:724629/207‑6 (MQ=255)                                                                                                                                                                                      tgcAAACCAAGCGTTTACCTGCCGATTGATCCAATTCAAATCCAATTCCTGCGTTTGCACCAGTACGCGCCGCCAATATAAATCGCCAGGCGAACGCTCATCGCCCATAGCGATTTTATTTAACAGGGAAAAACACTTGTCAACATCCACCGCACTTTCATCGGCAATCAGCTTCAAATACGCTTCAAGGTTTGTGGTCGGAATAAGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAAtt                                                    <  1:66508/249‑1 (MQ=255)                                                                                                                                                                                            ccAAGCGTTTACCTGCCGATTGATCCAATTCAAATCCAATTCCTGCGTTTGCACCAGTACGCGCCGCCAATATAAATCGCCAGGCGAACGCTCATCGCCCATAGCGATTTTATTTAACAGGGAAAAACACTTGTCAACATCCACCGCACTTTCATCGGCAATCAGCTTCAAATACGCTTCAAGGTTTGTGGTCGGAATAAGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTgtag                                               >  1:137454/1‑246 (MQ=255)                                                                                                                                                                                                 cGTTTACCTGCCGATTGATCCAATTCAAATCCAATTCCTGCGTTTGCACCAGTACGCGCCGCCAATATAAATCGCCAGGCGAACGCTCATCGCCCATAGCGATTTTATTTAACAGGGAAAAACACTTGTCAACATCCACCGCACTTTCATCGGCAATCAGCTTCAAATACGCTTCAAGGTTTGTGGTCGGAATAAGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTgtagc                                              <  1:591780/245‑5 (MQ=255)                                                                                                                                                                                                 cGTTTACCTGCCGATTGATCCAATTCAAATCCAATTCCTGCGTTTGCACCAGTACGCGCCGCCAATATAAATCGCCAGGCGAACGCTCATCGCCCATAGCGATTTTATTTAACAGGGAAAAACACTTGTCAACATCCACCGCACTTTCATCGGCAATCAGCTTCAAATACGCTTCAAGGTTTGTGGTCGGAATAAGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGTAGCTGGGCa                                        >  1:253975/1‑251 (MQ=255)                                                                                                                                                                                                     tgccTGCCGATTGGTCCAATTCAAATCCAATTCCTGCGTTTGCACCAGTACGCGCCGCCAATATAAATCGCCAGGCGAACGCTCATCGCCCATAGCGATTTTATTTAACAGGGAAAAACACTTGTCAACATCCACCGCACTTTCATCGGCAATCAGCTTCAAATACGCTTCAAGGTTTGTGGTCGGAATAAGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGTAGCTGGGCAACgg                                    <  1:413859/249‑1 (MQ=255)                                                                                                                                                                                                       ccTGCCGATTGATCCAATTCAAATCCAATTCCTGCGTTTGCACCAGTACGCGCCGCCAATATAAATCGCCAGGCGAACGCTCATCGCCCATAGCGATTTTATTTAACAGGGAAAAACACTTGTCAACATCCACCGCACTTTCATCGGCAATCAGCTTCAAATACGCTTCAAGGTTTGTGGTCGGAATAAGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCg                                  >  1:343079/1‑251 (MQ=255)                                                                                                                                                                                                                  aTCCAATTCAAATCCAATTCCTGCGTTTGCACCAGTACGCGCCGCCAATATAAATCGCCAGGCGAACGCTCATCGCCCATAGCGATTTTATTTAACAGGGAAAAACACTTGTCAACATCCACCGCACTTTCATCGGCAATCAGCTTCAAATACGCTTCAAGGTTTGTGGTCGGAATAAGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAgg                       <  1:316995/251‑1 (MQ=255)                                                                                                                                                                                                                   tCCAATTCAAATCCAATTCCTGCGTTTGCACCAGTACGCGCCGCCAATATAAATCGCCAGGCGAACGCTCATCGCCCATAGCGATTTTATTTAACAGGGAAAAACACTTGTCAACATCCACCGCACTTTCATCGGCAATCAGCTTCAAATACGCTTCAAGGTTTGTGGTCGGAATAAGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGc                      <  1:244997/251‑1 (MQ=255)                                                                                                                                                                                                                               ccAATTCCTGCGTTTGCACCAGTACGCGCCGCCAATATAAATCGCCAGGCGAACGCTCATCGCCCATAGCGATTTTATTTAACAGGGAAAAACACTTGTCAACATCCACCGCACTTTCATCGGCAATCAGCTTCAAATACGCTTCAAGGTTTGTGGTCGGAATAAGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAAc          <  1:348541/251‑1 (MQ=255)                                                                                                                                                                                                                                cAATTCCTGCGTTTGCACCAGTACGCGCCGCCAATATAAATCGCCAGGCGAACGCTCATCGCCCATAGCGATTTTATTTAACAGGGAAAAACACTTGTCAACATCCACCGCACTTTCATCGGCAATCAGCTTCAAATACGCTTCAAGGTTTGTGGTCGGAATAAGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAAcc         <  1:300787/251‑1 (MQ=255)                                                                                                                                                                                                                                  aTTCCTGCGTTTGCACCAGTACGCGCCGCCAATATAAATCGCCAGGCGAACGCTCATCGCCCATAGCGATTTTATTTAACAGGGAAAAACACTTGTCAACATCCACCGCACTTTCATCGGCAATCAGCTTCAAATACGCTTCAAGGTTTGTGGTCGGAATAAGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCg        >  1:189472/1‑250 (MQ=255)                                                                                                                                                                                                                                        gCGTTTGCACCAGTACGCGCCGCCAATATAAATCGCCAGGCGAACGCTCATCGCCCATAGCGATTTTATTTAACAGGGAAAAACACTTGTCAACATCCACCGCACTTTCATCGGCAATCAGCTTCAAATACGCTTCAAGGTTTGTGGTCGGAATAAGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCAcgcg  >  1:60331/1‑250 (MQ=255)                                                                                                                                                                                                                                          gTTTGCACCAGTACGCGCCGCCAATATAAATCGCCAGGCGAACGCTCATCGCCCATAGCGATTTTATTTAACAGGGAAAAACACTTGTCAACATCCACCGCACTTTCATCGGCAATCAGCTTCAAATACGCTTCAAGGt                                                                                                               >  1:460348/1‑139 (MQ=255)                                                                                                                                                                                                                                            ttGCACCAGTACGCGCCGCCAATATAAATCGCCAGGCGAACGCTCATCGCCCATAGCGATTTTATTTAACAGGGAAAAACACTTGTCAACATCCACCGCACTTTCATCGGCAATCAGCTTCAAATACGCTTCAAGGTTTGTGGTCGGAATAAGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGc                      <  1:188966/226‑1 (MQ=255)                                                                                                                                                                                                                                                   aGTACGCGCCGCCAATATAAATCGCCAGGCGAACGCTCATCGCCCATAGCGATTTTATTTAACAGGGAAAAACACTTGTCAACATCCACCGCACTTTCATCGGCAATCAGCTTCAAATACGCTTCAAGGTTTGTGGTCGGAATAAGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGTAGCTGGGCAACGGCGTCCTGTTGAGGCAACACATTaa            <  1:337895/229‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                          TTGTGGCTGGAAGTGCGCGAATCCAATAAAAAAGCCCAAGCGCTGTATGATTCGCTGGGCTTTAATCAGGTGGATATTCGCAAAAATTATTATCCCACCCCCGACGGCAAACGGGAAAATGCCGTGGTGATGGCGGCGTATTTATAGGCTATTTTTCCCTTTTGATAGCTTGCAACATCGGCGCAAACCAAGCGTTTACCTGCCGATTGATCCAATTCAAATCCAATTCCTGCGTTTGCACCAGTACTCGCCGCCAATATAAATCGCCAGGCGAACGCTCATCGCTCATAGCGATTTTATTTAACAGGGAAAAACACTTGTCAATATCCACCGCACTTTCATCGGCAATCAACTTCAAATACGCTTCAAGGTTTGTGGTCGGAACCAGCTGTAATTGCGTCCGCCCCGCCAAATAACTTTCCACATAAATTTGCAGTTGGGCAACGGCGTCCTGTTGAGGCAACACATTAAACCGCACGCG  >  1/1830188‑1830668

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑