Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,830,113	C→A	intergenic (+48213/+5271)	unknown → / ← unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,830,113	0	C	A	100.0%	187.9 / NA	63	intergenic (+48213/+5271)	unknown/unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (36/27);  total (36/27)

CGATAAAGTAAGGATTCGCCGTGATACACATTTCTCCTATTGAACCCTCGGATTTTGATCGTCTTTATCTCATTGAGCAGGCGGCACACGCGGTGCCTTGGAGCTTAGGCACGCTGAAAAATAATCAGGGCAAACGTTACCTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGTTTTGCCATTTGCCAGACGGTGTTGGATGAAGCCACGCTGTTTAATATCGCCGTGGATCCGCAACAGCAGGGATTGGGGC‑TGGGGCGCCGCTTATTAAGCGAGCTGATGACACAATTAAAACAAAAAAGCATTCTGACCTTGTGGCTGGAAGTGCGCGAATCCAATAAAAAAGCCCAAGCGCTGTATGATTCGCTGGGCTTTAATCAGGTGGATATTCGCAAAAATTATTATCCCACCCCCGACGGCAAACGGGAAAATGCCGTGGTGATGGCGGCGTATTTATAGGCTA  >  1/1829871‑1830338                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                   cGATAAAGTAAGGATTCGCCGTGATACACATTTCTCCTATTGAACCCTCGGATTTTGATCGTCTTTATCTCATTGAGCAGGCGGCACACGCGATGCCTTGGAGCTTAGGCACACTGAAAAATAATCAGGGCGAACGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGATGAAGCCACGCTGTTTAATATCGCCTTGGATCCGCAAAAACAgggg                                                                                                                                                                                                                            <  1:413324/251‑2 (MQ=255)      aaGTAAGGATTCGCCGTGATACACATTTCTCCTATTGAACCCTCGGATTTTGATCGTCTTTATCTCATTGAGCAGGCGGCACACGCGATGCCTTGGAGCTTAGGCACACTGAAAAATAATCAGGGCGAACGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGATGAAGCCACGCTGTTTAATATCGCCTTGGATCCGCAAAAACAggggcgggg                                                                                                                                                                                                                       >  1:345849/1‑245 (MQ=255)              aTTCGCCGTGATACACATTTCTCCTATTGAACCCTCGGATTTTGATCGTCTTTATCTCATTGAGCAGGCGGCACACGCGATGCCTTGGAGCTTAGGCACACTGAAAAATAATCAGGGCGAACGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGATGAAGCCACGCTGTTTAATATCGCCTTGGATCCGCAAAAACAggggcggggattgggg                                                                                                                                                                                                                <  1:231467/250‑14 (MQ=255)                     gTGATACACATTTCTCCTATTGAACCCTCGGATTTTGATCGTCTTTATCTCATTGAGCAGGCGGCACACGCGATGCCTTGGAGCTTAGGCACACTGAAAAATAATCAGGGCGAACGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGATGAAGCCACGCTGTTTAATATCGCCTTGGATCCGCAAAAACAggggcggggattggggc                                                                                                                                                                                                               >  1:578348/1‑230 (MQ=255)                       gATACACATTTCTCCTATTGAACCCTCGGATTTTGATCGTCTTTATCTCATTGAGCAGGCGGCACACGCGATGCCTTGGAGCTTAGGCACACTGAAAAATAATCAGGGCGAACGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGATGAAGCCACGCTGTTTAATATCGCCTTGGATCCGCAAAAACAggggcggggattggggcgccgtttat                                                                                                                                                                                                      >  1:713178/1‑228 (MQ=255)                       gATACACATTTCTCCTATTGAACCCTCGGATTTTGATCGTCTTTATCTCATTGAGCAGGCGGCACACGCGATGCCTTGGAGCTTAGGCACACTGAAAAATAATCAGGGCGAACGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGATGAAGCCACGCTGTTTAATATCGCCTTGGATCCGCAAAAACAggggcgggga                                                                                                                                                                                                                      <  1:204331/235‑8 (MQ=255)                          acacATTTCTCCTATTGAACCCTCGGATTTTGATCGTCTTTATCTCATTGAGCAGGCGGCACACGCGATGCCTTGGAGCTTAGGCACACTGAAAAATAATCAGGGCGAACGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGATGAAGCCACGCTGTTTAATATCGCCTTGGATCCGCAAAAACAGGGGCGGGGAT‑TGGGGCGCCGTTTATTaa                                                                                                                                                                                                  >  1:393649/1‑251 (MQ=255)                           cacaTTTCTCCTATTGAACCCTCGGATTTTGATCGTCTTTATCTCATTGAGCAGGCGGCACACGCGATGCCTTGGAGCTTAGGCACACTGAAAAATAATCAGGGCGAACGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGATGAAGCCACGCTGTTTAATATCGCCTTGGATCCGCAAAAACAGGGGCGGGGAT‑TGGGGCGCCGTTTATTaac                                                                                                                                                                                                 <  1:643239/251‑2 (MQ=255)                             caTTTCTCCTATTGAACCCTCGGATTTTGATCGTCTTTATCTCATTGAGCAGGCGGCACACGCGATGCCTTGGAGCTTAGGCACACTGAAAAATAATCAGGGCGAACGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGATGAAGCCACGCTGTTTAATATCGCCTTGGATCCGCAAAAACAggggcggggattgggg                                                                                                                                                                                                                >  1:110481/1‑222 (MQ=255)                             caTTTCTCCTATTGAACCCTCGGATTTTGATCGTCTTTATCTCATTGAGCAGGCGGCACACGCGATGCCTTGGAGCTTAGGCACACTGAAAAATAATCAGGGCGAACGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGATGAAGCCACGCTGTTTAATATCGCCTTGGATCCGCAAAAACAggggcggggat                                                                                                                                                                                                                     >  1:526756/1‑222 (MQ=255)                                    ccTATTGAACCCTCGGATTTTGATCGTCTTTATCTCATTGAGCAGGCAGCACACGCGATGCCTTGGAGCTTAGGCACACTGAAAAATAATCAGGGCGAACGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGATGAAGCCACGCTGTTTAATATCGCCTTGGATCCGCAAAAACAGGGGCGGGGAT‑TGGGGCGCCGTTTATTAACCGAGCtgat                                                                                                                                                                                        >  1:655582/1‑251 (MQ=255)                                     cTATTGAACCCTCGGATTTTGATCGTCTTTATCTCATTGAGCAGGCGGCACACGCGATGCCTTGGAGCTTAGGCACACTGAAAAATAATCAGGGCGAACGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGATGAAGCCACGCTGTTTAATATCGCCTTGGATCCGCAAAAACAGGGGCGGGGAT‑TGGGGCGCCGTTTATTAACCGAGCtgatg                                                                                                                                                                                       >  1:602064/1‑251 (MQ=255)                                       aTTGAACCCTCGGATTTTGATCGTCTTTATCTCATTGAGCAGGCGGCACACGCGATGCCTTGGAGCTTAGGCACACTGAAAAATAATCAGGGCGAACGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGATGAAGCCACGCTGTTTAATATCGCCTTGGATCCGCAAAAACAGGGGCGGGGAT‑TGGGGCGCCGTTTATTAACCGAGCtgatga                                                                                                                                                                                      >  1:150127/1‑250 (MQ=255)                                         tGAACCCTCGGATTTTAATCGTCTTTATCTCATTGAGCAGGCGGCACACGCGATGCCTTGGAGCTTAGGCACACTGAAAAATAATCAGGGCGAACGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGATGAAGCCACGCTGTTTAATATCGCCTTGGATCCGCAAAAACAGGGGCGGGGAT‑TGGGGCGCCGTTTATTAACCGAGCTGATGacgc                                                                                                                                                                                   <  1:65212/251‑3 (MQ=255)                                                    aTTTTGATCGTCTTTATCTCATTGAGCAGGCGGCACACGCGATGCCTTGGAGCTTAGGCACACTGAAAAATAATCAGGGCGAACGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGATGAAGCCACGCTGTTTAATATCGCCTTGGATCCGCAAAAACAGGGGCGGGGAT‑TGGGGCGCCGTTTATTAACCGAGCTGATGACGCAGTTAAAACa                                                                                                                                                                         <  1:133945/250‑1 (MQ=255)                                                       ttGATCGTCTTTATCTCATTGAGCAGGCGGCACACGCGATGCCTTGGAGCTTAGGCACACTGAAAAATAATCAGGGCGAACGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGATGAAGCCACGCTGTTTAATATCGCCTTGGATCCGCAAAAACAGGGGCGGGGAT‑TGGGGCGCCGTTTATTAACCGAGCTGATGACGCAGTTAAAACaaaaa                                                                                                                                                                     >  1:548931/1‑251 (MQ=255)                                                              tCTTTATCTCATTGAGCAGGCGGCACACGCGATGCCTTGGAGCTTAGGCACACTGAAAAATAATCAGGGCGAACGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGATGAAGCCACGCTGTTTAATATCGCCTTGGATCCGCAAAAACAGGGGCGGGGAT‑TGGGGCGCCGTTTATTAACCGAGCTGATGACGCAGTTAAAACAAAAAGGCAttt                                                                                                                                                              >  1:5945/1‑250 (MQ=255)                                                                  tATCTCATTGAGCAGGCGGCACACGCGATGCCTTGGAGCTTAGGCACACTGAAAAATAATCAGGGCGAACGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGATGAAGCCACGCTGTTTAATATCGCCTTGGATCCGCAAAAACAGGGGCGGGGAT‑TGGGGCGCCGTTTATTAACCGAGCTGATGACGCAGTTAAAACAAAAAGGCATTTTGAc                                                                                                                                                          <  1:9096/251‑1 (MQ=255)                                                                      tcATTGAGCAGGCGGCACACGCGATGCCTTGGAGCTTAGGCACACTGAAAAATAATCAGGGCGAACGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGATGAAGCCACGCTGTTTAATATCGCCTTGGATCCGCAAAAACAGGGGCGGGGAT‑TGGGGCGCCGTTTATTAACCGAGCTGATGACGCAGTTAAAACAAAAAGGCATTTTGACCTtg                                                                                                                                                      >  1:584400/1‑251 (MQ=255)                                                                       cATTGAGCAGGCGGCACACGCGATGCCTTGGAGCTTAGGCACACTGAAAAATAATCAGGGCGAACGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGATGAAGCCACGCTGTTTAATATCGCCTTGGATCCGCAAAAACAGGGGCGGGGAT‑TGGGGCGCCGTTTATTAACCGAGCTGATGACGCAGTTAAAACAAAAAGGCATTTTGACCTtgt                                                                                                                                                     >  1:55893/1‑251 (MQ=255)                                                                       cATTGAGCAGGCGGCACACGCGATGCCTTGGAGCCTAGGCACACTGAAAAATAATCAGGGCGAACGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGATGAAGCCACGCTGTTTAATATCGCCTTGGATCCGCAAAAACAGGGGCGGGGAT‑TGGGGCGCCGTTTATTAACCGAGCTGATGACGCAGTTAAAACAAAAAGGCATTTTGACCTtgt                                                                                                                                                     >  1:605146/1‑251 (MQ=255)                                                                           gAGCAGGCGGCACACGCGATGCCTTGGAGCTTAGGCACACTGAAAAATAATCAGGGCGAACGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGATGAAGCCACGCTGTTTAATATCGCCTTGGATCCGCAAAAACAGGGGCGGGGAT‑TGGGGCGCCGTTTATTAACCGAGCTGATGACGCAGTTAAAACAAAAAGGCATTTTGACCTTGTgg                                                                                                                                                   >  1:845669/1‑249 (MQ=255)                                                                                 gcggcACACGCGATGCCTTGGAGCTTAGGCACACTGAAAAATAATCAGGGCGAACGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGATGAAGCCACGCTGTTTAATATCGCCTTGGATCCGCAAAAACAGGGGCGGGGAT‑TGGGGCGCCGTTTATTAACCGAGCTGATGACGCAGTTAAAACAAAAAGGCATTTTGACCTTGTGGCTGGa                                                                                                                                              <  1:683865/248‑1 (MQ=255)                                                                                 gcggcACACGCGATGCCTTGGAGCTTAGGCACACTGAAAAATAATCAGGGCGAACGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGATGAAGCCACGCTGTTTAATATCGCCTTGGATCCGCAAAAACAGGGGCGGGGAT‑TGGGGCGCCGTTTATTAACCGAGCTGATGACGCAGTTAAAACAAAAAGGCATTTTGACCTTGTGGCTGGAAg                                                                                                                                            <  1:72509/250‑1 (MQ=255)                                                                                 gcggcACACGCGATGCCTTGGAGCTTAGGCACACTGAAAAATAATCAGGGCGAACGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGATGAAGCCACGCTGTTTAATATCGCCTTGGATCCGCAAAAACAGGGGCGGGGAT‑TGGGGCGCCGTTTATTAACCGAGCTGATGACGCAGTTAAAACAAAAAGGCATTTTGACCTTGTGGCTGGAAg                                                                                                                                            <  1:549617/250‑1 (MQ=255)                                                                                  cggcACACGCGATGCCTTGGAGCTTAGGCACACTGAAAAATAATCAGGGCGAACGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGATGAAGCCACGCTGTTTAATATCGCCTTGGATCCGCAAAAACAGGGGCGGGGAT‑TGGGGCGCCGTTTATTAACCGAGCTGATGACGCAGTTAAAACaa                                                                                                                                                                        <  1:262892/221‑1 (MQ=255)                                                                                  cggcACACGCGATGCCTTGGAGCTTAGGCACACTGAAAAATAATCAGGGCGAACGCTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGATGAAGGCACGCTGTTTAATATCGCCTTGGATCCGCAAAAACAGGGGCGGGGAT‑TGGGGCGCCGTTTATTAACCGAGCTGATGACGCAGTTAAAACAAAAAGGCATTTTGACCTTGTGGCTGGAAg                                                                                                                                            <  1:161887/249‑1 (MQ=255)                                                                                             atGCCTTGGAGCTTAGGCACACTGAAAAATAATCAGGGCGAACGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGATGAAGCCACGCTGTTTAATATCGCCTTGGATCCGCAAAAACAGGGGCGGGGAT‑TGGGGCGCCGTTTATTAACCGAGCTGATGACGCAGTTAAAACAAAAAGGCATTTTGACCTTGTGGCTGGAAGTGCGCGAATCCaa                                                                                                                               >  1:204685/2‑251 (MQ=255)                                                                                                    ggAGCTTAGGCACACTGAAAAATAATCAGGGCGAACGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGATGAAGCCACGCTGTTTAATATCGCCTTGGATCCGCAAAAACAGGGGCGGGGAT‑TGGGGCGCCGTTTATTaac                                                                                                                                                                                                 >  1:677310/1‑177 (MQ=255)                                                                                                       gCTTAGGCACACTGAAAAATAATCAGGGCGAACGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGATGAAGCCACGCTGTTTAATATCGCCTTGGATCCGCAAAAACAGGGGCGGGGAT‑TGGGGCGCCGTTTATTAACCGAGCTGATGACGCAGTTAAAACAAAAAGGCATTTTGACCTTGTGGCTGGAAGTGCGCGAATCCAATAAAAAAGc                                                                                                                      >  1:118782/1‑250 (MQ=255)                                                                                                                cacTGAAAAATAATCAGGGCGAACGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGATGAAGCCACGCTGTTTAATATCGCCTTGGATCCGCAAAAACAGGGGCGGGGAT‑TGGGGCGCCGTTTATTAACCGAGCTGATGACGCAGTTAAAACAAAAAGGCATTTTGACCTTGTGGCTGGAAGTGCGCGAATCCAATaaaaa                                                                                                                         <  1:4302/236‑1 (MQ=255)                                                                                                                  cTGAAAAATAATCAGGGCGAACGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGATGAAGCCACGCTGTTTAATATCGCCTTGGATCCGCAAAAACAGGGGCGGGGAT‑TGGGGCGCCGTTTATTaac                                                                                                                                                                                                 <  1:574246/164‑2 (MQ=255)                                                                                                                   tGAAAAATAATCAGGGCGAACGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGATGAAGCCACGCTGTTTAATATCGCCTTGGATCCGCAAAAACAGGGGCGGGGAT‑TGGGGCGCCGTTTATTAACCGAGCTGATGACGCAGTTAAAACAAAAAGGCATTTTGACCTTGTGGCTGGAAGTGCGCGAATCCAATAAAAAAGccctggcactgtat                                                                                                         <  1:25992/251‑12 (MQ=255)                                                                                                                    gAAAAATAATCAGGGCGAACGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGATGAAGCCACGCTGTTTAATATCGCCTTGGATCCGCAAAAACAGGGGCGGGGAT‑TGGGGCGCCGTTTATTAACCGAGCTGATGACGCAGTTAAAACAAAAAGGCATTTTGACCTTGTGGCTGGAAGTGCGCGAATCCAATAAAAAAGCCCTGGCACTGTATg                                                                                                        >  1:200657/1‑251 (MQ=255)                                                                                                                      aaaaTAATCAGGGCGAACGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGATGAAGCCACGCTGTTTAATATCGCCTTGGATCCGCAAAAACAGGGGCGGGGAT‑TGGGGCGCCGTTTATTAACCGAGCTGATGACGCCGTTAAAACAAAAAGGCATTTTGACCTTGTGGCTGGAAGTGCGCGAATCCAATAAAAAAGccctggcactgtattat                                                                                                      >  1:572578/1‑237 (MQ=255)                                                                                                                      aaaaTAATCAGGGCGAACGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGATGAAGCCACGCTGTTTAATATCGCCTTGGATCCGCAAAAACAGGGGCGGGGAT‑TGGGGCGCCGTTTATTAACCGAGCTGATGACGCAGTTAAAACAAAAAGGCATTTTGACCTTGTGGCTGGAAGTGCGCGAATCCAATAAAAAAGCCCTGGCACTGTATGAt                                                                                                      <  1:802180/251‑1 (MQ=255)                                                                                                                      aaaaTAATCAGGGCGAACGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGATGAAGCCACGCTGTTTAATATCGCCTTGGATCCGCAAAAACAGGG‑GCGGGGCTTGGGGCGCCGTTTATTAACCGAGCTGATGACGCAGTTAAAACAAAAAGGCATTTTGACCTTGTGGCTGGAAGTGCGCGAATCCAATAAAAAAGCCCTGGCACTGTATGaa                                                                                                      >  1:384132/1‑250 (MQ=255)                                                                                                                        aataatCAGGGCGAACGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGATGAAGCCACGCTGTTTAATATCGCCTTGGATCCGCAAAAACAGGGGCGGGGAT‑TGGGGCGCCGTTTATTAACCGAGCTGATGACGCAGTTAAAACAAAAAGGCATTTTGACCTTGTGGCTGGAAGTGCGCGAATCCAATAAAAAAGCCCTGGCACTGTATGATTc                                                                                                    <  1:768447/251‑1 (MQ=255)                                                                                                                                gggCGAACGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGATGAAGCCACGCTGTTTAATATCGCCTTGGATCCGCAAAAACAGGGGCGGGGAT‑TGGGGCGCCGTTTATTAACCGAGCTGATGACGCAGTTAAAACAAAAAGGCATTTTGACCTTGTGGCTGGAAGTGCGCGAATCCAATAAAAAAGccctggcactgtat                                                                                                         >  1:218227/1‑227 (MQ=255)                                                                                                                                       cGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGATGAAGCCACGCTGTTTAATATCGCCTTGGATCCGCAAAAACAGGGGCGGGGAT‑TGGGGCGCCGTTTATTAACCGAGCTGATGACGCAGTTAAAACAAAAAGGCATTTTGACCTTGTGGCTGGAAGTGCGCGAATCCAATAAAAAAGCCCTGGCACTGTATGATTCGCTGGGCTTTAATCa                                                                                     >  1:499775/1‑251 (MQ=255)                                                                                                                                       cGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGATGAAGCCACGCTGTTTAATATCGCCTTGGATCCGCAAAAACAGGGGCGGGGAT‑TGGGGCGCCGTTTATTAACCGAGCTGATGACGCAGTTAAAACAAAAAGGCATTTTGACCTTGTGGCTGGAAGTGCGCGAATCCAATAAAAAAGCCCTGGCACTGTATGATTAGCTGGGCTTTAATCa                                                                                     >  1:613529/1‑251 (MQ=255)                                                                                                                                               cAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGATGAAGCCACGCTGTTTAATATCGCCTTGGATCCGCAAAAACAGGGGCGGGGAT‑TGGGGCGCCGTTTATTAACCGAGCTGATGACGCAGTTAAAACAAAAAGGCATTTTGACCTTGTGGCTGGAAGTGCGCGAATCCAATAAAAAAGCCCTGGCACTGTATGATTCGCTGGGCTTTAATCAAGTGGata                                                                             <  1:681648/251‑1 (MQ=255)                                                                                                                                                            ggCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGATGAAGCCACGCTGTTTAATATCGCCTTGGATCCGCAAAAACAGGGGCGGGGAT‑TGGGGCGCCGTTTATTAACCGAGCTGATGACGCAGTTAAAACAAAAAGGCATTTTGACCTTGTGGCTGGAAGTGCGCGAATCCAATAAAAAAGCCCTGGCACTGTATGATTCGCTGGGCTTTAATCAAGTGGATATTCGCAAAAatta                                                                >  1:105220/1‑251 (MQ=255)                                                                                                                                                                        cGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGATGAAGCCACGCTGTTTAATATCGCCTTGGATCCGCAAAAACAGGGGCGGGGAT‑TGGGGCGCCGTTTATTAACCGAGCTGATGACGCAGTTAAAACAAAAAGGCATTTTGACCTTGTGGCTGGAAGTGCGCGAATCCAATAAAAAAGCCCTGGCACTGTATGATTCGCTGGGCTTTAATCAAGTGGATATTCGCAAAAATTATTATCCCAccgc                                                    <  1:121398/251‑3 (MQ=255)                                                                                                                                                                                  gctttgctatttgccaAACGGTGTTGGATGAAGCCACGCTGTTTAATATCGCCTTGGATCCGCAAAAACAGGGGCGGGGAT‑TGGGGCGCCGTTTATTaaccg                                                                                                                                                                                               <  1:357735/100‑4 (MQ=255)                                                                                                                                                                                  gctttgctatttgccaAACGGTGTTGGATGAAGCCACGCTGTTTAATATCGCCTTGGATCCGCAAAAACAGGGGCGGGGAT‑TGGGGCGCCGTTTATTAACCGAGCTGATGACGCAGTTAAAACAAAAAGGCATTTTGACCTTGTGGCTGGAAGTGCGCGAATCCAATAAAAAAGCCCTGGCACTGTATGATTCGCTGGGCTTTAATCaag                                                                                   <  1:728274/208‑3 (MQ=255)                                                                                                                                                                                  gctttgctatttgccaAACGGTGTTGGATGAAGCCACGCTGTTTAATATCGCCTTGGATCCGCAAAAACAGGGGCGGGGAT‑TGGGGCGCCGTTTATTAACCGAGCTGATGACGCAGTTAAAACAAAAAGGCATTTTGACCTTGTGGCTGGAAGTGCGCGAATCCAATAAAAAAGCCCTGGCACTGTATGATTCGCTGGGCTTTAATCAAGTGGATATTCGCAAAAATTATTATCCCACCGCCGACGGCaaa                                          >  1:648754/3‑251 (MQ=255)                                                                                                                                                                                  gctttgctatttgccaAACGGTGTTGGATGAAGCCACGCTGTTTAATATCGCCTTGGATCCGCAAAAACAGGGGCGGGGAT‑TGGGGCGCCGTTTATTAACCGAGCTGATGACGCAGTTAAAACAAAAAGGCATTTTGACCTTGTGGCTGGAAGTGCGCGAATCCAATAAAAAAGCCCTGGCACTGTATGATTCGCTGGGCTTTAATCAAGTGGATATTCGCAAAAATTATTATCCCACCGCCGACGGCaaa                                          >  1:652779/3‑251 (MQ=255)                                                                                                                                                                                        ctatttgccaAACGGTGTTGGATGAAGCCACGCTGTTTAATATCGCCTTGGATCCGCAAAAACAGGGGCGGGGAT‑TGGGGCGCCGTTTATTAACCGAGCTGATGACGCAGTTAAAACAAAAAGGCATTTTGACCTTGTGGCTGGAAGTGCGCGAATCCAATAAAAAAGccctggca                                                                                                               >  1:84851/3‑171 (MQ=255)                                                                                                                                                                                                      gtgtTGGATGAAGCCACGCTGTTTAATATCGCCTTGGATCCGCAAAAACAGGGGCGGGGAT‑TGGGGCGCCGTTTATTAACCGAGCTGATGACGCAGTTAAAACAAAAAGGCATTTTGACCTTGTGGCTGGAAGTGCGCGAATCCAATAAAAAAGccctggcac                                                                                                              >  1:538825/1‑157 (MQ=255)                                                                                                                                                                                                      gtgtTGGATGAAGCCACGCTGTTTAATATCGCCTTGGATCCGCAAAAACAGGGGCGGGGAT‑TGGGGCGCCGTTTATTAACCGAGCTGATGACGCAGATAAAACAAAAAGGCATTTTGACCTTGTGGCTGGAAGTGCGCGAATCCAATAAAAAAGCCCTGGCACTGTATGATTCGCTGGGCTTTAATCAAGTGGATATTCGCAAAAATTATTATCCCACCGCCGACGGCAAACGGGaaa                                   <  1:104871/238‑1 (MQ=255)                                                                                                                                                                                                                aaGCCACGCTGTTTAATATCGCCTTGGATCCGCAAAAACAGGGGCGGGGAT‑TGGGGCGCCGTTTATTAACCGAGCTGATGACGCAGTTAAAACAAAAAGGCATTTTGACCTTTTGGCTGGAAGTGCGCGAATCCAATAAAAAAGCCCTGGCACTTTATGATTCCCTGGGCTTTAATCAAGTGGATATTCGCAAAAATTATTATCCCACCGCCGCCGGCAAACGGGAAAAAGCCggggttactgctgcggat            >  1:526347/1‑238 (MQ=255)                                                                                                                                                                                                                    cACGCTGTTTAATATCGCCTTGGATCCGCAAAAACAGGGGCGGGGAT‑TGGGGCGCCGTTTATTAACCGAGCTGATGACGCAGTTAAAACAAAAAGGCATTTTGACCTTGTGGCTGGAAGTGCGCGAATCCAATAAAAAAGCCCTGGCACTGTATGATTCGCTGGGCTTTAATCAAGTGGATATTCGCAAAAATTATTATCCCACCGCCGACGGCAAACGGGAAAATGCCGTGGTGATGGCGGCGTATTtat        <  1:770772/251‑1 (MQ=255)                                                                                                                                                                                                                     aCGCTGTTTAATATCGCCTTTGATCCGCAAAAACCGGGGCGGGGAT‑TGGGGCGCCGTTTATTAACCGAGCTGATGACGCAGTTAAAACAAAAAAGCATTTTGACCTTGTGGCTGGAAGTGCGCGAATCCCATAAAAAAGCCCTGGCACTGTATGATTc                                                                                                    <  1:736585/158‑1 (MQ=255)                                                                                                                                                                                                                          gTTTAATATCGCCTTGGATCCGCAAAAACAGGGGCGGGGAT‑TGGGGCGCCGTTTATTAACCGAGCTGATGACGCAGTTAAAACAAAAAGGCATTTTGACCTTGTGGCTGGAAGTGCGCGAATcc                                                                                                                                 <  1:890560/124‑1 (MQ=255)                                                                                                                                                                                                                          gTTTAATATCGCCTTGGATCCGCAAAAACAGGGGCGGGGAT‑TGGGGCGCCGTTTATTAACCGAGCTGATGACGCAGTTAAAACAAAAAGGCATTTTGACCTTGTGGCTGGAAGTGCGCGAATCCAATAAAAAAGCCCTGGCACTGTATGATTCGCTGGGCTTTAAt                                                                                       >  1:509131/1‑166 (MQ=255)                                                                                                                                                                                                                          gTTTAATATCGCCTTGGATCCGCAAAAACAGGGGCGGGGAT‑TGGGGCGCCGTTTATTAACCGAGCTGATGACGCAGTTAAAACAAAAAGGCATTTTGACCTTGTGGCTGGAAGTGCGCGAATCCAATAAAAAAGCCCTGGCACTGTATGATTCGCTGGGCTTTAATCAAGTGGATATTCGCAAAAATTTTTATCCCACCGCCGACGGCAAACGGGAAAATGCCGTGGTGATGGCGGCGTATTTATAGGCTa  >  1:154011/1‑251 (MQ=255)                                                                                                                                                                                                                          gTTTAATATCGCCTTGGATCCGCAAAAACAGGGGCGGGGAT‑TGGGGCGCCGTTTATTAACCGAGCTGATGACGCAGTTAAAACAAAAAGGCATTTTGACCTTGTGGCTGGAAGTGCGCGAATCCAATAAAAAAGCCCTGGCACTGTATGATTCGCTGGGCTTTAATCAAGTGGATATTCGCAAAAATTATTATCCCACCGCCGACGGCAAACGGGAAAATGc                               <  1:560325/222‑1 (MQ=255)                                                                                                                                                                                                                          gTTTAATATCGCCTTGGATCCGCAAAAACAGGGGCGGGGAT‑TGGGGCGCCGTTTATTAACCGAGCTGATGACGCAGTTAAAACAAAAAGGCATTTTGACCTTGTGGCTGGAAGTGCGCGAATCCAATAAAAAAGCCCTGGCACTGTATGATTCGCTGGGCTTTAATCAAGTGGATATTCGCAAAAATTATTATCCCACCGCCGACGGCAAACGGGAAAATGCCGTGGTGATGGCGGCGTATTTATAGGCTa  >  1:243692/1‑251 (MQ=255)                                                                                                                                                                                                                          gTTTAATATCGCCTTGGATCCGCAAAAACAGGGGCGGGGAT‑TGGGGCGCCGTTTATTAACCGAGCTGATGACGCAGTTAAAACAAAAAGGCATTTTGACCTTGTGGCTGGAAGTGCGCGAATCCAATAAAAAAGCCCTGGCACTGTATGATTCGCTGGGCTTTAATCAAGTGGATATTCGCAAAAATTATTATCCCACCGCCGACGGCAAACGGGAAAATGCCGGGGGGAGGGCGGGGTATTTTTAGGCtt  >  1:526513/1‑250 (MQ=255)                                                                                                                                                                                                                          gTTTAATATCGCCTTGGATCCGCAAAAACAGGGGCGGGGAT‑TGGGGCGCCGTTTATTAACCGAGCTGATGACGCAGTTAAAACAAAAAGGCATTTTGACCTTGTGGCTGGAAGTGCGCGAATCCAATAAAAAAACCCTGGCACTGTTTGATTCGCTGGGCTTTAATCAAGTGGATATTCGCAAAAATTATTATCCCACCGCCGACGGCAAACGGGAAAATGCCGTGGTGATGGCCGCGTATTTATAGGCTa  >  1:259826/1‑251 (MQ=255)                                                                                                                                                                                                                          gTTTAATATCGCCTTGGATCCGCAAAAACAGGGGCGGGGAT‑TGGGGCGCCGTTTATTAACCGAGCTGATGACGCAGTTAAAACAAAAAGGCATTTTGACCTTGTGGCTGGAAGTGCGCGAATCCAAAAAAAAACCCCTGGCACTGTATGATTTGCTGGGCCTTAATCAAGGGGATATTCGCAAAAATTATTATcc                                                          >  1:640892/1‑195 (MQ=255)                                                                                                                                                                                                                               atatCGCCTTGGATCCGCAAAAACAGGGGCGGGGAT‑TGGGGCGCCGTTTATTAACCGAGCTGATGACGCAGTTAAAACAAAAAGGCATTTTGACCTTGTGGCTGGAAGTGCGCGAATcc                                                                                                                                 <  1:110329/119‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                   CGATAAAGTAAGGATTCGCCGTGATACACATTTCTCCTATTGAACCCTCGGATTTTGATCGTCTTTATCTCATTGAGCAGGCGGCACACGCGGTGCCTTGGAGCTTAGGCACGCTGAAAAATAATCAGGGCAAACGTTACCTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGTTTTGCCATTTGCCAGACGGTGTTGGATGAAGCCACGCTGTTTAATATCGCCGTGGATCCGCAACAGCAGGGATTGGGGC‑TGGGGCGCCGCTTATTAAGCGAGCTGATGACACAATTAAAACAAAAAAGCATTCTGACCTTGTGGCTGGAAGTGCGCGAATCCAATAAAAAAGCCCAAGCGCTGTATGATTCGCTGGGCTTTAATCAGGTGGATATTCGCAAAAATTATTATCCCACCCCCGACGGCAAACGGGAAAATGCCGTGGTGATGGCGGCGTATTTATAGGCTA  >  1/1829871‑1830338

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑