Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,830,049	T→C	intergenic (+48149/+5335)	unknown → / ← unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,830,049	0	T	C	100.0%	240.2 / NA	79	intergenic (+48149/+5335)	unknown/unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (39/40);  total (39/40)

ATCCGCAGGCGAAACGCCAACTCTGGCGGCAAATTCAGCAATCCGCACCGATAAAGTAAGGATTCGCCGTGATACACATTTCTCCTATTGAACCCTCGGATTTTGATCGTCTTTATCTCATTGAGCAGGCGGCACACGCGGTGCCTTGGAGCTTAGGCACGCTGAAAAATAATCAGGGCAAACGTTACCTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGTTTTGCCATTTGCCAGACGGTGTTGGATGAAGCCACGCTGTTTAATATCGCCGTGGATCCGCAACAGCAGGGATTGGGGC‑TGGGGCGCCGCTTATTAAGCGAGCTGATGACACAATTAAAACAAAAAAGCATTCTGACCTTGTGGCTGGAAGTGCGCGAATCCAATAAAAAAGCCCAAGCGCTGTATGATTCGCTGGGCTTTAATCAGGTGGATATTCGCAAAAATTATTATCCCACCCC  >  1/1829823‑1830288                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                 aTCCGCAGGCGAAACGCCAACTCTGGCGGCAAATTCAGCAATCCGCACCGATAAAGTAAGGATTCGCCGTGATACACATTTCTCCTATTGAACCCTCGGATTTTGATCGTCTTTATCTCATTGAGCAGGCGGCACACGCGATGCCTTGGAGCTTAGGCACACTGAAAAATAATCAGGGCGAACGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTg                                                                                                                                                                                                                          <  1:463101/251‑1 (MQ=255)  tCCGCAGGCGAAACGCCAACTCTGGCGGCAAATTCAGCAATCCGCACCGATAAAGTAAGGATTCGCCGTGATACACATTTCTCCTATTGAACCCTCGGATTTTGATCGTCTTTATCTCATTGAGCAGGCGGCACACGCGATGCCTTGGAGCTTAGGCACACTGAAAAATAATCAGGGCGAACGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTgg                                                                                                                                                                                                                         >  1:514217/1‑251 (MQ=255)    cGCAGGCGAAACGCCAACTCTGGCGGCAAATTCAGCAATCCGCACCGATAAAGTAAGGATTCGCCGTGATACACATTTCTCCTATTGAACCCTCGGATTTTGATCGTCTTTATCTCATTGAGCAGGCGGCACACGCGATGCCTTGGAGCTTAGGCACACTGAAAAATAATCAGGGCGAACGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGAt                                                                                                                                                                                                                       <  1:773860/251‑1 (MQ=255)    cGCAGGCGAAACGCCAACTCTGGCGGCAAATTCAGCAATCCGCACCGATAAAGTAAGGATTCGCCGTGATACACATTTCTCCTATTGAACCCTCGGATTTTGATCGTCTTTATCTCATTGAGCAGGCGGCACACGCGATGCCTTGGAGCTTAGGCACACTGAAAAATAATCAGGGCGAACGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGAt                                                                                                                                                                                                                       <  1:696276/251‑1 (MQ=255)    cGCAGGCGAAACGCCAACTCTGGCGGCAAATTCAGCAATCCGCACCGATAAAGTAAGGATTCGCCGTGATACACATTTCTCCTATTGAACCCTCGGATTTTGATCGTCTTTATCTCATTGAGCAGGCGGCACACGCGATGCCTTGGAGCTTAGGCACACTGAAAAATAATCAGGGCGAACGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGAt                                                                                                                                                                                                                       <  1:305949/251‑1 (MQ=255)        ggCGAAACGCCAACTCTGGCGGCAAATTCAGCAATCCGCACCGATAAAGTAAGGATTCGCCGTGATACACATTTCTCCTATTGAACCCTCGGATTTTGATCGTCTTTATCTCATTGAGCAGGCGGCACACGCGATGCCTTGGAGCTTAGGCACACTGAAAAATAATCAGGGCGAACGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGATGAAg                                                                                                                                                                                                                   >  1:888725/1‑251 (MQ=255)              aCGCCAACTCTGGCGGCAAATTCAGCAATCCGCACCGATAAAGTAAGGATTCGCCGTGATACACATTTCTCCTATTGAACCCTCGGATTTTGATCGTCTTTATCTCATTGAGCAGGCGGCACACGCGATGCCTTGGAGCTTAGGCACACTGAAAAATAATCAGGGCGAACGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGATGAAGCCACGc                                                                                                                                                                                                             <  1:742410/251‑1 (MQ=255)                    aCTCTGGCGGCAAATTCAGCAATCCGCACCGATAAAGTAAGGATTCGCCGTGATACACATTTCTCCTATTGAACCCTCGGATTTTGATCGTCTTTATCTCATTGAGCAGGCGGCACACGCGATGCCTTGGAGCTTAGGCACACTGAAAAATAATCAGGGCGAACGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGATGAAGCCACGCTGTTTa                                                                                                                                                                                                       >  1:750922/1‑251 (MQ=255)                     ctctGGCGGCAAATTCAGCAATCCGCACCGATAAAGTAAGGATTCGCCGTGATACACATTTCTCCTATTGAACCCTCGGATTTTGATCGTCTTTATCTCATTGAGCAGGCGGCACACGCGATGCCTTGGAGCTTAGGCACACTGAAAAATAATCAGGGCGAACGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGAt                                                                                                                                                                                                                       >  1:734845/1‑234 (MQ=255)                      tctGGCGGCAAATTCAGCAATCCGCACCGATAAAGTAAGGATTCGCCGTGATACACATTTCTCCTATTGAACCCTCGGATTTTGATCGTCTTTATCTCATTGAGCAGGCGGCACACGCGATGCCTTGGAGCTTAGGCACGCTGAAAAATAATCAGGGCGAACGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTATTGGATGAAGCCACGCTGTTTAat                                                                                                                                                                                                     <  1:522213/251‑1 (MQ=255)                        tGGCGGCAAATTCAGCAATCCGCACCGATAAAGTAAGGATTCGCCGTGATACACATTTCTCCTATTGAACCCTCGGATTTTGATCGTCTTTATCTCATTGAGCAGGCGGCACACGCGATGCCTTGGAGCTTAGGCACACTGAAAAATAATCAGGGCGAACGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGATGAAGCCACGCTGTTTAatat                                                                                                                                                                                                   <  1:570320/251‑1 (MQ=255)                        tGGCGGCAAATTCAGCAATCCGCACCGATAAAGTAAGGATTCGCCGTGATACACATTTCTCCTATTGAACCCTCGGATTTTGATCGTCTTTATCTCATTGAGCAGGCGGCACACGCGATGCCTTGGAGCTTAGGCACACTGAAAAATAATCAGGGCGAACGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGATGAAGCCACGCTGTTTAatat                                                                                                                                                                                                   <  1:869091/251‑1 (MQ=255)                        tGGCGGCAAATTCAGCAATCCGCACCGATAAAGTAAGGATTCGCCGTGATACACATTTCTCCTATTGAACCCTCGGATTTTGATCGTCTTTATCTCATTGAGCAGGCGGCACACGCGATGCCTTGGAGCTTAGGCACACTGAAAAATAATCAGGGCGAACGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGATGAAGCCACGCTGTTTAatat                                                                                                                                                                                                   <  1:35640/251‑1 (MQ=255)                              cAAATTCAGCAATCCGCACCGATAAAGTAAGGATTCGCCGTGATACACATTTCTCCTATTGAACCCTCGGATTTTGATCGTCTTTATCTCATTGAGCAGGCGGCACACGCGATGCCTTGGAGCTTAGGCACACTGAAAAATAATCAGGGCGAACGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGATGAAGCCACGCTGTTTAATATCGcct                                                                                                                                                                                              <  1:473327/250‑2 (MQ=255)                                 aTTCAGCAATCCGCACCGATAAAGTAAGGATTCGCCGTGATACACATTTCTCCTATTGAACCCTCGGATTTTGATCGTCTTTATCTCATTGAGCAGGCGGCACACGCGATGCCTTGGAGCTTAGGCACACTGAAAAATAATCAGGGCGAACGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGATGAAGCCACGCTGTTTAat                                                                                                                                                                                                     >  1:280838/1‑240 (MQ=255)                                       cAATCCGCACCGATAAAGTAAGGATTCGCCGTGATACACATTTCTCCTATTGAGCCCTCGGATTTTGATCGTCTTTATCTCATTGAGCAGGCGGCACACGCGATGCCTTGGAGCTTAGGCACACTGAAAAATAATCAGGGCGAACGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGATGAAGCCACGCTGTTTAATATCGCCTTGGATCCGc                                                                                                                                                                                     <  1:863009/250‑1 (MQ=255)                                                 cGATAAAGTAAGGATTCGCCGTGATACACATTTCTCCTATTGAACCCTCGGATTTTGATCGTCTTTATCTCATTGAGCAGGCGGCACACGCGATGCCTTGGAGCTTAGGCACACTGAAAAATAATCAGGGCGAACGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGATGAAGCCACGCTGTTTAATATCGCCTTGGATCCGCAAAAACAgggg                                                                                                                                                                          <  1:413324/251‑2 (MQ=255)                                                  gATAAAGTAAGGATTCGCCGTGATACACATTTCTCCTATTGAACCCTCGGATTTTGATCGTCTTTATCTCATTGAGCAGGCGGCACACGCGATGCCTTGGAGCTTAGGCACACTGAAAAATAATCAGGGCGAACGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGATGAAGCCACGCTGTTTAATATCGCCTTGGa                                                                                                                                                                                          >  1:560998/1‑234 (MQ=255)                                                      aaGTAAGGATTCGCCGTGATACACATTTCTCCTATTGAACCCTCGGATTTTGATCGTCTTTATCTCATTGAGCAGGCGGCACACGCGATGCCTTGGAGCTTAGGCACACTGAAAAATAATCAGGGCGAACGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGAt                                                                                                                                                                                                                       >  1:220139/1‑201 (MQ=255)                                                      aaGTAAGGATTCGCCGTGATACACATTTCTCCTATTGAACCCTCGGATTTTGATCGTCTTTATCTCATTGAGCAGGCGGCACACGCGATGCCTTGGAGCTTAGGCACACTGAAAAATAATCAGGGCGAACGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGATGAAGCCACGCTGTTTAATATCGCCTTGGATc                                                                                                                                                                                        >  1:642581/1‑232 (MQ=255)                                                      aaGTAAGGATTCGCCGTGATACACATTTCTCCTATTGAACCCTCGGATTTTGATCGTCTTTATCTCATTGAGCAGGCGGCACACGCGATGCCTTGGAGCTTAGGCACACTGAAAAATAATCAGGGCGAACGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGATGAAGCCACGCTGTTTAATATCGCCTTGGATCCGCAAAAACAggggcgggg                                                                                                                                                                     >  1:345849/1‑245 (MQ=255)                                                       aGTAAGGATTCGCCGTGATACACATTTCTCCTATTGAACCCTCGGATTTTGATCGTCTTTATCTCATTGAGCAGGCGGCACACGCGATGCCTTGGAGCTTAGGCACACTGAAAAATAATCAGGGCGAACGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGATGAAGCCACGCTGTTTAATATCGCCTTGGATc                                                                                                                                                                                        <  1:140225/231‑1 (MQ=255)                                                              aTTCGCCGTGATACACATTTCTCCTATTGAACCCTCGGATTTTGATCGTCTTTATCTCATTGAGCAGGCGGCACACGCGATGCCTTGGAGCTTAGGCACACTGAAAAATAATCAGGGCGAACGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGATGAAGCCACGCTGTTTAATATCGCCTTGGATCCGCAAAAACAggggcggggattgggg                                                                                                                                                              <  1:231467/250‑14 (MQ=255)                                                                     gTGATACACATTTCTCCTATTGAACCCTCGGATTTTGATCGTCTTTATCTCATTGAGCAGGCGGCACACGCGATGCCTTGGAGCTTAGGCACACTGAAAAATAATCAGGGCGAACGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGATGAAGCCACGCTGTTTAATATCGCCTTGGATCCGCAAAAACAggggcggggattggggc                                                                                                                                                             >  1:578348/1‑230 (MQ=255)                                                                       gATACACATTTCTCCTATTGAACCCTCGGATTTTGATCGTCTTTATCTCATTGAGCAGGCGGCACACGCGATGCCTTGGAGCTTAGGCACACTGAAAAATAATCAGGGCGAACGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGATGAAGCCACGCTGTTTAATATCGCCTTGGATCCGCAAAAACAggggcggggattggggcgccgtttat                                                                                                                                                    >  1:713178/1‑228 (MQ=255)                                                                       gATACACATTTCTCCTATTGAACCCTCGGATTTTGATCGTCTTTATCTCATTGAGCAGGCGGCACACGCGATGCCTTGGAGCTTAGGCACACTGAAAAATAATCAGGGCGAACGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGATGAAGCCACGCTGTTTAATATCGCCTTGGATCCGCAAAAACAggggcgggga                                                                                                                                                                    <  1:204331/235‑8 (MQ=255)                                                                          acacATTTCTCCTATTGAACCCTCGGATTTTGATCGTCTTTATCTCATTGAGCAGGCGGCACACGCGATGCCTTGGAGCTTAGGCACACTGAAAAATAATCAGGGCGAACGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGATGAAGCCACGCTGTTTAATATCGCCTTGGATCCGCAAAAACAGGGGCGGGGAT‑TGGGGCGCCGTTTATTaa                                                                                                                                                >  1:393649/1‑251 (MQ=255)                                                                           cacaTTTCTCCTATTGAACCCTCGGATTTTGATCGTCTTTATCTCATTGAGCAGGCGGCACACGCGATGCCTTGGAGCTTAGGCACACTGAAAAATAATCAGGGCGAACGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGATGAAGCCACGCTGTTTAATATCGCCTTGGATCCGCAAAAACAGGGGCGGGGAT‑TGGGGCGCCGTTTATTaac                                                                                                                                               <  1:643239/251‑2 (MQ=255)                                                                             caTTTCTCCTATTGAACCCTCGGATTTTGATCGTCTTTATCTCATTGAGCAGGCGGCACACGCGATGCCTTGGAGCTTAGGCACACTGAAAAATAATCAGGGCGAACGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGATGAAGCCACGCTGTTTAATATCGCCTTGGATCCGCAAAAACAggggcggggattgggg                                                                                                                                                              >  1:110481/1‑222 (MQ=255)                                                                             caTTTCTCCTATTGAACCCTCGGATTTTGATCGTCTTTATCTCATTGAGCAGGCGGCACACGCGATGCCTTGGAGCTTAGGCACACTGAAAAATAATCAGGGCGAACGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGATGAAGCCACGCTGTTTAATATCGCCTTGGATCCGCAAAAACAggggcggggat                                                                                                                                                                   >  1:526756/1‑222 (MQ=255)                                                                                  ctcCTATTGAACCCTCGGATTTTGATCGTCTTTATCTCATTGAGCAGGCGGCACACGCGATGCCTTGGAGCTTAGGCACACTGAAAAATAATCAGGGCGAACGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGAt                                                                                                                                                                                                                       <  1:310877/173‑1 (MQ=255)                                                                                  ctcCTATTGAACCCTCGGATTTTGATCGTCTTTATCTCATTGAGCAGGCGGCACACGCGATGCCTTGGAGCTTAGGCACACTGAAAAATAATCAGGGCGAACGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGATGAAGCCACGCTGTTTAATATCGCCTTGGa                                                                                                                                                                                          >  1:818360/1‑202 (MQ=255)                                                                                    ccTATTGAACCCTCGGATTTTGATCGTCTTTATCTCATTGAGCAGGCAGCACACGCGATGCCTTGGAGCTTAGGCACACTGAAAAATAATCAGGGCGAACGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGATGAAGCCACGCTGTTTAATATCGCCTTGGATCCGCAAAAACAGGGGCGGGGAT‑TGGGGCGCCGTTTATTAACCGAGCtgat                                                                                                                                      >  1:655582/1‑251 (MQ=255)                                                                                     cTATTGAACCCTCGGATTTTGATCGTCTTTATCTCATTGAGCAGGCGGCACACGCGATGCCTTGGAGCTTAGGCACACTGAAAAATAATCAGGGCGAACGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGATGAAGCCACGCTGTTTAatat                                                                                                                                                                                                   <  1:422262/190‑1 (MQ=255)                                                                                     cTATTGAACCCTCGGATTTTGATCGTCTTTATCTCATTGAGCAGGCGGCACACGCGATGCCTTGGAGCTTAGGCACACTGAAAAATAATCAGGGCGAACGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGATGAAGCCACGCTGTTTAATATCGCCTTGGATCCGCAAAAACAGGGGCGGGGAT‑TGGGGCGCCGTTTATTAACCGAGCtgatg                                                                                                                                     >  1:602064/1‑251 (MQ=255)                                                                                       aTTGAACCCTCGGATTTTGATCGTCTTTATCTCATTGAGCAGGCGGCACACGCGATGCCTTGGAGCTTAGGCACACTGAAAAATAATCAGGGCGAACGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCtttgcta                                                                                                                                                                                                                                           <  1:294117/148‑3 (MQ=255)                                                                                       aTTGAACCCTCGGATTTTGATCGTCTTTATCTCATTGAGCAGGCGGCACACGCGATGCCTTGGAGCTTAGGCACACTGAAAAATAATCAGGGCGAACGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGATGAAGCCACGCTGTTTAat                                                                                                                                                                                                     >  1:713396/1‑186 (MQ=255)                                                                                       aTTGAACCCTCGGATTTTGATCGTCTTTATCTCATTGAGCAGGCGGCACACGCGATGCCTTGGAGCTTAGGCACACTGAAAAATAATCAGGGCGAACGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGATGAAGCCACGCTGTTTAATATCGCCTTGGATCCGCAAAAACAGGGGCGGGGAT‑TGGGGCGCCGTTTATTAACCGAGCtgatga                                                                                                                                    >  1:150127/1‑250 (MQ=255)                                                                                         tGAACCCTCGGATTTTAATCGTCTTTATCTCATTGAGCAGGCGGCACACGCGATGCCTTGGAGCTTAGGCACACTGAAAAATAATCAGGGCGAACGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGATGAAGCCACGCTGTTTAATATCGCCTTGGATCCGCAAAAACAGGGGCGGGGAT‑TGGGGCGCCGTTTATTAACCGAGCTGATGacgc                                                                                                                                 <  1:65212/251‑3 (MQ=255)                                                                                                   gATTTTGATCGTCTTTATCTCATTGAGCAGGCGGCACACGCGATGCCTTGGAGCTTAGGCACACTGAAAAATAATCAGGGCGAACGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGATGAAGCCACGCTGTTTAatat                                                                                                                                                                                                   >  1:777172/1‑176 (MQ=255)                                                                                                   gATTTTGATCGTCTTTATCTCATTGAGCAGGCGGCACACGCGATGCCTTGGAGCTTAGGCACACTGAAAAATAATCAGGGCGAACGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGATGAAGCCACGCTGTTTAATATCGCCTTGGATc                                                                                                                                                                                        <  1:109703/187‑1 (MQ=255)                                                                                                   gATTTTGATCGTCTTTATCTCATTGAGCAGGCGGCACACGCGATGCCTTGGAGCTTAGGCACACTGAAAAATAATCAGGGCGAACGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGATGAAGCCACGCTGTTTAATATCGCCTTGGATc                                                                                                                                                                                        >  1:130204/1‑187 (MQ=255)                                                                                                    aTTTTGATCGTCTTTATCTCATTGAGCAGGCGGCACACGCGATGCCTTGGAGCTTAGGCACACTGAAAAATAATCAGGGCGAACGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGATGAAGCCACGCTGTTTAATATCGCCTTGGATCCGCAAAAACAGGGGCGGGGAT‑TGGGGCGCCGTTTATTAACCGAGCTGATGACGCAGTTAAAACa                                                                                                                       <  1:133945/250‑1 (MQ=255)                                                                                                     ttttGATCGTCTTTATCTCATTGAGCAGGCGGCACACGCGATGCCTTGGAGCTTAGGCACACTGAAAAATAATCAGGGCGAACGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGATGAAGCCACGCTGTTTAatat                                                                                                                                                                                                   >  1:260347/1‑174 (MQ=255)                                                                                                       ttGATCGTCTTTATCTCATTGAGCAGGCGGCACACGCGATGCCTTGGAGCTTAGGCACACTGAAAAATAATCAGGGCGAACGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGATGAAGCCACGCTGTTTAATATCGCCTTGGATCCGCAAAAACAGGGGCGGGGAT‑TGGGGCGCCGTTTATTAACCGAGCTGATGACGCAGTTAAAACaaaaa                                                                                                                   >  1:548931/1‑251 (MQ=255)                                                                                                             gTCTTTATCTCATTGAGCAGGCGGCACACGCGATGCCTTGGAGCTTAGGCACACTGAAAAATAATCAGGGCGAACGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGAt                                                                                                                                                                                                                       <  1:156084/146‑1 (MQ=255)                                                                                                              tCTTTATCTCATTGAGCAGGCGGCACACGCGATGCCTTGGAGCTTAGGCACACTGAAAAATAATCAGGGCGAACGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGATGAAGCCACGCTGTTTAatat                                                                                                                                                                                                   <  1:416310/165‑1 (MQ=255)                                                                                                              tCTTTATCTCATTGAGCAGGCGGCACACGCGATGCCTTGGAGCTTAGGCACACTGAAAAATAATCAGGGCGAACGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGATGAAGCCACGCTGTTTAATATCGCCTTGGATCCGCAAAAACAGGGGCGGGGAT‑TGGGGCGCCGTTTATTAACCGAGCTGATGACGCAGTTAAAACAAAAAGGCAttt                                                                                                            >  1:5945/1‑250 (MQ=255)                                                                                                                  tATCTCATTGAGCAGGCGGCACACGCGATGCCTTGGAGCTTAGGCACACTGAAAAATAATCAGGGCGAACGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGATGAAGCCACGCTGTTTAATATCGCCTTGGATCCGCAAAAACAGGGGCGGGGAT‑TGGGGCGCCGTTTATTAACCGAGCTGATGACGCAGTTAAAACAAAAAGGCATTTTGAc                                                                                                        <  1:9096/251‑1 (MQ=255)                                                                                                                   aTCTCATTGAGCAGGCGGCACACGCGATGCCTTGGAGCTTAGGCACACTGAAAAATAATCAGGGCGAACGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGATGAAGCCACGCTGTTTAat                                                                                                                                                                                                     <  1:466529/158‑1 (MQ=255)                                                                                                                      tcATTGAGCAGGCGGCACACGCGATGCCTTGGAGCTTAGGCACACTGAAAAATAATCAGGGCGAACGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGATGAAGCCACGCTGTTTAATATCGCCTTGGATCCGCAAAAACAGGGGCGGGGAT‑TGGGGCGCCGTTTATTAACCGAGCTGATGACGCAGTTAAAACAAAAAGGCATTTTGACCTtg                                                                                                    >  1:584400/1‑251 (MQ=255)                                                                                                                       cATTGAGCAGGCGGCACACGCGATGCCTTGGAGCTTAGGCACACTGAAAAATAATCAGGGCGAACGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGATGAAGCCACGCTGTTTAATATCGCCTTGGATCCGCAAAAACAGGGGCGGGGAT‑TGGGGCGCCGTTTATTAACCGAGCTGATGACGCAGTTAAAACAAAAAGGCATTTTGACCTtgt                                                                                                   >  1:55893/1‑251 (MQ=255)                                                                                                                       cATTGAGCAGGCGGCACACGCGATGCCTTGGAGCCTAGGCACACTGAAAAATAATCAGGGCGAACGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGATGAAGCCACGCTGTTTAATATCGCCTTGGATCCGCAAAAACAGGGGCGGGGAT‑TGGGGCGCCGTTTATTAACCGAGCTGATGACGCAGTTAAAACAAAAAGGCATTTTGACCTtgt                                                                                                   >  1:605146/1‑251 (MQ=255)                                                                                                                           gAGCAGGCGGCACACGCGATGCCTTGGAGCTTAGGCACACTGAAAAATAATCAGGGCGAACGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGATg                                                                                                                                                                                                                      <  1:655018/133‑1 (MQ=255)                                                                                                                           gAGCAGGCGGCACACGCGATGCCTTGGAGCTTAGGCACACTGAAAAATAATCAGGGCGAACGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGATGAAGCCACGCTGTTTAATATCGCCTTGGATCCGCAAAAACAGGGGCGGGGAT‑TGGGGCGCCGTTTATTAACCGAGCTGATGACGCAGTTAAAACAAAAAGGCATTTTGACCTTGTgg                                                                                                 >  1:845669/1‑249 (MQ=255)                                                                                                                                 gcggcACACGCGATGCCTTGGAGCTTAGGCACACTGAAAAATAATCAGGGCGAACGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGATGAAGCCACGCTGTTTAATATCGCCTTGGATCCGCAAAAACAGGGGCGGGGAT‑TGGGGCGCCGTTTATTAACCGAGCTGATGACGCAGTTAAAACAAAAAGGCATTTTGACCTTGTGGCTGGa                                                                                            <  1:683865/248‑1 (MQ=255)                                                                                                                                 gcggcACACGCGATGCCTTGGAGCTTAGGCACACTGAAAAATAATCAGGGCGAACGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGATGAAGCCACGCTGTTTAATATCGCCTTGGATCCGCAAAAACAGGGGCGGGGAT‑TGGGGCGCCGTTTATTAACCGAGCTGATGACGCAGTTAAAACAAAAAGGCATTTTGACCTTGTGGCTGGAAg                                                                                          <  1:549617/250‑1 (MQ=255)                                                                                                                                 gcggcACACGCGATGCCTTGGAGCTTAGGCACACTGAAAAATAATCAGGGCGAACGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGATGAAGCCACGCTGTTTAATATCGCCTTGGATCCGCAAAAACAGGGGCGGGGAT‑TGGGGCGCCGTTTATTAACCGAGCTGATGACGCAGTTAAAACAAAAAGGCATTTTGACCTTGTGGCTGGAAg                                                                                          <  1:72509/250‑1 (MQ=255)                                                                                                                                  cggcACACGCGATGCCTTGGAGCTTAGGCACACTGAAAAATAATCAGGGCGAACGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGATGAAGCCACGCTGTTTAATATCGCCTTGGATCCGCAAAAACAGGGGCGGGGAT‑TGGGGCGCCGTTTATTAACCGAGCTGATGACGCAGTTAAAACaa                                                                                                                      <  1:262892/221‑1 (MQ=255)                                                                                                                                  cggcACACGCGATGCCTTGGAGCTTAGGCACACTGAAAAATAATCAGGGCGAACGCTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGATGAAGGCACGCTGTTTAATATCGCCTTGGATCCGCAAAAACAGGGGCGGGGAT‑TGGGGCGCCGTTTATTAACCGAGCTGATGACGCAGTTAAAACAAAAAGGCATTTTGACCTTGTGGCTGGAAg                                                                                          <  1:161887/249‑1 (MQ=255)                                                                                                                                             atGCCTTGGAGCTTAGGCACACTGAAAAATAATCAGGGCGAACGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGATGAAGCCACGCTGTTTAATATCGCCTTGGATCCGCAAAAACAGGGGCGGGGAT‑TGGGGCGCCGTTTATTAACCGAGCTGATGACGCAGTTAAAACAAAAAGGCATTTTGACCTTGTGGCTGGAAGTGCGCGAATCCaa                                                                             >  1:204685/2‑251 (MQ=255)                                                                                                                                                ccTTGGAGCTTAGGCACACTGAAAAATAATCAGGGCGAACGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGATGAAGCCACGCTGTTTAATATCGCCTTGGATCCGCaaaaac                                                                                                                                                                               >  1:222688/1‑147 (MQ=255)                                                                                                                                                    ggAGCTTAGGCACACTGAAAAATAATCAGGGCGAACGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGATGAAGCCACGCTGTTTAATATCGCCTTGGATCCGCAAAAACAGGGGCGGGGAT‑TGGGGCGCCGTTTATTaac                                                                                                                                               >  1:677310/1‑177 (MQ=255)                                                                                                                                                       gCTTAGGCACACTGAAAAATAATCAGGGCGAACGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGATGAAGCCACGCTGTTTAATATCGCCTTGGATCCGCAAAAACAGGGGCGGGGAT‑TGGGGCGCCGTTTATTAACCGAGCTGATGACGCAGTTAAAACAAAAAGGCATTTTGACCTTGTGGCTGGAAGTGCGCGAATCCAATAAAAAAGc                                                                    >  1:118782/1‑250 (MQ=255)                                                                                                                                                          tAGGCACACTGAAAAATAATCAGGGCGAACGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGATGAAGCCACGCTGTTTAatat                                                                                                                                                                                                   <  1:91965/121‑1 (MQ=255)                                                                                                                                                                cacTGAAAAATAATCAGGGCGAACGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGATGAAGCCACGCTGTTTAATATCGCCTTGGATCCGCAAAAACAGGGGCGGGGAT‑TGGGGCGCCGTTTATTAACCGAGCTGATGACGCAGTTAAAACAAAAAGGCATTTTGACCTTGTGGCTGGAAGTGCGCGAATCCAATaaaaa                                                                       <  1:4302/236‑1 (MQ=255)                                                                                                                                                                  cTGAAAAATAATCAGGGCGAACGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGATGAAGCCACGCTGTTTAATATCGCCTTGGATCCGCAAAAACAGGGGCGGGGAT‑TGGGGCGCCGTTTATTaac                                                                                                                                               <  1:574246/164‑2 (MQ=255)                                                                                                                                                                   tGAAAAATAATCAGGGCGAACGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGATGAAGCCACGCTGTTTAATATCGCCTTGGATCCGCAAAAACAGGGGCGGGGAT‑TGGGGCGCCGTTTATTAACCGAGCTGATGACGCAGTTAAAACAAAAAGGCATTTTGACCTTGTGGCTGGAAGTGCGCGAATCCAATAAAAAAGccctggcactgtat                                                       <  1:25992/251‑12 (MQ=255)                                                                                                                                                                    gAAAAATAATCAGGGCGAACGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGATGAAGCCACGCTGTTTAATATCGCCTTGGATCCGCAAAAACAGGGGCGGGGAT‑TGGGGCGCCGTTTATTAACCGAGCTGATGACGCAGTTAAAACAAAAAGGCATTTTGACCTTGTGGCTGGAAGTGCGCGAATCCAATAAAAAAGCCCTGGCACTGTATg                                                      >  1:200657/1‑251 (MQ=255)                                                                                                                                                                      aaaaTAATCAGGGCGAACGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGATGAAGCCACGCTGTTTAATATCGCCTTGGATCCGCAAAAACAGGGGCGGGGAT‑TGGGGCGCCGTTTATTAACCGAGCTGATGACGCCGTTAAAACAAAAAGGCATTTTGACCTTGTGGCTGGAAGTGCGCGAATCCAATAAAAAAGccctggcactgtattat                                                    >  1:572578/1‑237 (MQ=255)                                                                                                                                                                      aaaaTAATCAGGGCGAACGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGATGAAGCCACGCTGTTTAATATCGCCTTGGATCCGCAAAAACAGGGGCGGGGAT‑TGGGGCGCCGTTTATTAACCGAGCTGATGACGCAGTTAAAACAAAAAGGCATTTTGACCTTGTGGCTGGAAGTGCGCGAATCCAATAAAAAAGCCCTGGCACTGTATGAt                                                    <  1:802180/251‑1 (MQ=255)                                                                                                                                                                      aaaaTAATCAGGGCGAACGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGATGAAGCCACGCTGTTTAATATCGCCTTGGATCCGCAAAAACAGGG‑GCGGGGCTTGGGGCGCCGTTTATTAACCGAGCTGATGACGCAGTTAAAACAAAAAGGCATTTTGACCTTGTGGCTGGAAGTGCGCGAATCCAATAAAAAAGCCCTGGCACTGTATGaa                                                    >  1:384132/1‑250 (MQ=255)                                                                                                                                                                        aataatCAGGGCGAACGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGATGAAGCCACGCTGTTTAATATCGCCTTGGATCCGCAAAAACAGGGGCGGGGAT‑TGGGGCGCCGTTTATTAACCGAGCTGATGACGCAGTTAAAACAAAAAGGCATTTTGACCTTGTGGCTGGAAGTGCGCGAATCCAATAAAAAAGCCCTGGCACTGTATGATTc                                                  <  1:768447/251‑1 (MQ=255)                                                                                                                                                                                gggCGAACGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGATGAAGCCACGCTGTTTAATATCGCCTTGGATCCGCAAAAACAGGGGCGGGGAT‑TGGGGCGCCGTTTATTAACCGAGCTGATGACGCAGTTAAAACAAAAAGGCATTTTGACCTTGTGGCTGGAAGTGCGCGAATCCAATAAAAAAGccctggcactgtat                                                       >  1:218227/1‑227 (MQ=255)                                                                                                                                                                                       cGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGATGAAGCCACGCTGTTTAATATCGCCTTGGATCCGCAAAAACAGGGGCGGGGAT‑TGGGGCGCCGTTTATTAACCGAGCTGATGACGCAGTTAAAACAAAAAGGCATTTTGACCTTGTGGCTGGAAGTGCGCGAATCCAATAAAAAAGCCCTGGCACTGTATGATTCGCTGGGCTTTAATCa                                   >  1:499775/1‑251 (MQ=255)                                                                                                                                                                                       cGTTACTTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGATGAAGCCACGCTGTTTAATATCGCCTTGGATCCGCAAAAACAGGGGCGGGGAT‑TGGGGCGCCGTTTATTAACCGAGCTGATGACGCAGTTAAAACAAAAAGGCATTTTGACCTTGTGGCTGGAAGTGCGCGAATCCAATAAAAAAGCCCTGGCACTGTATGATTAGCTGGGCTTTAATCa                                   >  1:613529/1‑251 (MQ=255)                                                                                                                                                                                               cAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGATGAAGCCACGCTGTTTAATATCGCCTTGGATCCGCAAAAACAGGGGCGGGGAT‑TGGGGCGCCGTTTATTAACCGAGCTGATGACGCAGTTAAAACAAAAAGGCATTTTGACCTTGTGGCTGGAAGTGCGCGAATCCAATAAAAAAGCCCTGGCACTGTATGATTCGCTGGGCTTTAATCAAGTGGata                           <  1:681648/251‑1 (MQ=255)                                                                                                                                                                                                            ggCGAAGAAAGCCGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGATGAAGCCACGCTGTTTAATATCGCCTTGGATCCGCAAAAACAGGGGCGGGGAT‑TGGGGCGCCGTTTATTAACCGAGCTGATGACGCAGTTAAAACAAAAAGGCATTTTGACCTTGTGGCTGGAAGTGCGCGAATCCAATAAAAAAGCCCTGGCACTGTATGATTCGCTGGGCTTTAATCAAGTGGATATTCGCAAAAatta              >  1:105220/1‑251 (MQ=255)                                                                                                                                                                                                                        cGAATCGACGGCTTTGCTATTTGCCAAACGGTGTTGGATGAAGCCACGCTGTTTAATATCGCCTTGGATCCGCAAAAACAGGGGCGGGGAT‑TGGGGCGCCGTTTATTAACCGAGCTGATGACGCAGTTAAAACAAAAAGGCATTTTGACCTTGTGGCTGGAAGTGCGCGAATCCAATAAAAAAGCCCTGGCACTGTATGATTCGCTGGGCTTTAATCAAGTGGATATTCGCAAAAATTATTATCCCAccgc  <  1:121398/251‑3 (MQ=255)                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                 ATCCGCAGGCGAAACGCCAACTCTGGCGGCAAATTCAGCAATCCGCACCGATAAAGTAAGGATTCGCCGTGATACACATTTCTCCTATTGAACCCTCGGATTTTGATCGTCTTTATCTCATTGAGCAGGCGGCACACGCGGTGCCTTGGAGCTTAGGCACGCTGAAAAATAATCAGGGCAAACGTTACCTCAATTTGAAAATCGGCGAAGAAAGCCGAATCGACGGTTTTGCCATTTGCCAGACGGTGTTGGATGAAGCCACGCTGTTTAATATCGCCGTGGATCCGCAACAGCAGGGATTGGGGC‑TGGGGCGCCGCTTATTAAGCGAGCTGATGACACAATTAAAACAAAAAAGCATTCTGACCTTGTGGCTGGAAGTGCGCGAATCCAATAAAAAAGCCCAAGCGCTGTATGATTCGCTGGGCTTTAATCAGGTGGATATTCGCAAAAATTATTATCCCACCCC  >  1/1829823‑1830288

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑