Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,829,626	T→C	intergenic (+47726/+5758)	unknown → / ← unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,829,626	0	T	C	100.0%	202.4 / NA	67	intergenic (+47726/+5758)	unknown/unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (34/33);  total (34/33)

TCCTATGGCTTATTGGTTAAAATGGGCGCCCATTATACTGGATTGAATTGATTTTAGATATGCAAAAAACGCGCCGACACACTTCCCGCCGAGATTTATTATTGCAGCAAATGGGCATCAGCCAATGGCAATTGGTGCGCCCTGAAGCCTTAAAAGGCGCGATTAATATCGTCGTGCCCGAATCGGTTCGTTTGGTTATCGTGAGCCATCAAAATCTGTTGCAACAACCCATCATACAGGATCTGTTGCGCAGTCTCACACTTGATAGCACGCAAGTGCTGAATATCACCTTTGAGCAGCTTGCCTATTTGCAAGTCAACCACACCGTCAATTATTGGCTACTCCATAAAAACGCCGATGAAATCCACCGCACTTTAAGCGGGCTGCAACAGCCGTTGAATGTCTGGCAATCCGTCGATCTCGCCGCGTTTAAACATGATCCGCAGGCGAAACGCCAACTCTGGCGGCAAATTCAGCAA  >  1/1829385‑1829863                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                              tCCTATGGCTTATTGGTTAAAATGGGCGCCCATTATACTGGATTGAATTGATTTTAGATATGCAAGAAACGCGCCGACACACTTCCCGCCGAGATTTATTATTGCAGCAAATGGGCATCAGCCAATGGCAATTGGTGCGCCCTGAAGCCTTAAAAGGTGCGATTAATATCGCCGTGCCCGAGTCGGTTCGTTTGGTTATCGTGAGCCATCAAAATCTGTTGCAACAACCCATCGCGCAGGACCTGTTgcg                                                                                                                                                                                                                                       >  1:661254/1‑250 (MQ=255) tCCTATGGCTTATTGGTTAAAATGGGCGCCCATTATACTGGATTGAATTGATTTTAGATATGCAAGAAACGCGCCGACACACTTCCCGCCGAGATTTATTATTGCAGCAAATGGGCATCAGCCAATGGCAATTGGTGCGCCCTGAAGCCTTAAAAGGCGCGATTAATATCGCCGTGCCCGAGTCGGTTCGTTTGGTTATCGTGAGCCATCAACATCTGTTGCAACAACCCCTCGCGCAGGACCTGTTgcg                                                                                                                                                                                                                                       >  1:868281/1‑250 (MQ=255)           tATTGGTTAAAATGGGCGCCCATTATACTGGATTGAATTGATTTTAGATATGCAAGAAACGCGCCGACACACTTCCCGCCGAGATTTATTATTGCAGCAAATGGGCATCAGCCAATGGCAATTGGTGCGCCCTGAAGCCTTAAAAGGCGCGATTAATATCGCCGTGCCCGAGTCGGTTCGTTTGGTTATCGTGAGCCATCAAAATCTGTTGCAACAACCCATCGCGCAGGACCTGTTGCGCGCTCTCacac                                                                                                                                                                                                                            <  1:217763/251‑1 (MQ=255)             ttGGTTAAAATGGGCGCCCATTATACTGGATTGAATTGATTTTAGATATGCAAGAAACGCGCCGACACACTTCCCGCCGAGATTTATTATTGCAGCAAATGGGCATCAGCCAATGGCAATTGGTGCGCCCTGAAGCCTTAAAAGGCGCGATTAACATCGCCGTGCCCGAGTCGGTTCGTTTGGTTATCGTGAGCCATCAAAATCTGTTGCAACAACCCATCGCGCAGGACCTGTTGCGCGCTCTCACACtt                                                                                                                                                                                                                          <  1:370930/251‑1 (MQ=255)               ggTTAAAATGGGCGCCCATTATACTGGATTGAATTGATTTTAGATATGCAAGAAACGCGCCGACACACTTCCCGCCGAGATTTATTATTGCAGCAAATGGGCATCAGCCAATGGCAATTGGTGCGCCCTGAAGCCTTAAAAGGCGCGATTAATATCGCCGTGCCCGAGTCGGTTCGTTTGGTTATCGTGAGCCATCAAAATCTGTTGCAACAACCCATCGCGCAGGACCTGTTGCGCGCTCTCACACTTGa                                                                                                                                                                                                                        >  1:741787/1‑251 (MQ=255)               ggTTAAAATGGGCGCCCATTATACTGGATTGAATTGATTTTAGATATGCAAGAAACGCGCCGACACACTTCCCGCCGAGATTTATTATTGCAGCAAATGGGCATCAGCCAATGGCAATTGGTGCGCCCTGAAGCCTTAAAAGGCGCGATTAATATCGCCGTGCCCGAGTCGGTTCGTTTGGTTATCGTGAGCCATCAAAATCTGTTGCAACAACCCATCGCGCAGGACCTGTTGCGCGCTCTCACACTTGa                                                                                                                                                                                                                        >  1:78348/1‑251 (MQ=255)                 ttAAAATGGGCGCCCATTATACTGGATTGAATTGATTTTAGATATGCAAGAAACGCGCCGACACACTTCCCGCCGAGATTTATTATTGCAGCAAATGGGCATCAGCCAATGGCAATTGGTGCGCCCTGAAGCCTTAAAAGGCGCGATTAATATCGCCGTGCCCGAGTCGGTTCGTTTGGTTATCGTGAGCCATCAAAATCTGTTGCAACAACCCATCGCGCAGGACCTGTTGCGCGCTCTCACACTTGATa                                                                                                                                                                                                                      <  1:697691/251‑1 (MQ=255)                        gggCGCCCATTATACTGGATTGAATTGATTTTAGATATGCAAGAAACGCGCCGACACACTTCCCGCCGAGATTTATTATTGCAGCAAATGGGCATCAGCCAATGGCAATTGGTGCGCCCTGAAGCCTTAAAAGGCGCGATTAATATCGCCGTGCCCGAGTCGGTTCGTTTGGTTATCGTGAGCCATCAAAATCTGTTGCAACAACCCATCGCGCAGGACCTGTTGCGCGCTCTCACACTTGATAGCACGc                                                                                                                                                                                                                <  1:605403/250‑1 (MQ=255)                         ggccccctttATACTGGATTGAATTGATTTTAGATATGCAAGAAACGCGCCGACCCACTTCCCGCCGAGATTTATTATTGCAGCAAATGGGCATCAGCCAATGGCAATTGGTGCGCCCTGAAGCCTTAAAAGGCGCGATTAATATCGCCGTGCCCGAGTCGGTTCGTTTGGTTATCGTGAGCCATCAAAATCTGTTGCAACAACCCATCGCGCAGGACCTGTTGCGCGCTCTCACACTTGATAGCACGCa                                                                                                                                                                                                               <  1:552503/242‑1 (MQ=255)                             cccATTATACTGGATTGAATTGATTTTAGATATGCAAGAAACGCGCCGACACACTTCCCGCCGAGATTTATTATTGCAGCAAATGGGCATCAGCCAATGGCAATTGGTGCGCCCTGAAGCCTTAAAAGGCGCGATTAATATCGCCGTGCCCGAGTCGGTTCGTTTGGTTATCGTGAGCCATCAAAATCTGTTGCAACAACCCATCGCGCAGGACCTGTTGCGCGCTCTCACACTTGATAGCACGCAAGTg                                                                                                                                                                                                           >  1:85605/1‑250 (MQ=255)                             cccATTATACTGGATTGAATTGATTTTAAATATGCAAGAAACGCGCCGACACACTTCCCGCCGAGATTTATTATTGCAGCAAATGGGCATCAGCCAATGGCAATTGGTGCGCCCTGAAGCCTTAAAAGGCGCGATTAATATCGCCGTGCCCGAGTCGGTTCGTTTGGTTATCGTGAGCCATCAAAATCTGTTGCAACAACCCATCGCGCAGGACCTGTTGCGCGCTCTCACACTTGATAGCACg                                                                                                                                                                                                                 <  1:580155/244‑1 (MQ=255)                              ccATTATACTGGATTGAATTGATTTTAGATATGCAAGAAACGCGCCGACACACTTCCCGCCGAGATTTATTATTGCAGCAAATGGGCATCAGCCAATGGCAATTGGTGCGCCCTGAAGCCTTAAAAGGCGCGATTAATATCGCCGTGCCCGAGTCGGTTCGTTTGGTTATCGTGAGCCATCAAAATCTGTTGCAACAACCCATCGCGCAGGACCTGTTGCGCGCTCTCACACtt                                                                                                                                                                                                                          >  1:682896/1‑234 (MQ=255)                               cATTATCCTGGATTGAATTTATTTAAGATATGCAAGAAACGCGCCGACACACTTCCCGCCGAGATTTATTATTGCAGCAAATGGGCATCAGCCAATGGCAATTGGTGCGCCCTGAAGCCTTAAAAGGCGCGATTAATATCGCCGTGCCCGAGTCGGTTCGTTTGGTTATCGTGAGCCATCAAAATCTGTTGCAACAACCCATCGCGCAGGACCTGTTGCGCGCTCTCACACTTGATAGCACGCAAGTGCTg                                                                                                                                                                                                        <  1:384692/251‑1 (MQ=255)                                  tataCTGGATTGAATTGATTTTAGATATGCAAGAAACGCGCCGACACACTTCCCGCCGAGATTTATTATTGCAGCAAATGGGCATCAGCCAATGGCAATTGGTGCGCCCTGAAGCCTTAAAAGGCGCGATTAATATCGCCGTGCCCGAGTCGGTTCGTTTGGTTATCGTGAGCCATCAAAATCTGTTGCAACAACCCATCGCGCAGGACCTGTTGCGCGCTCTCACACTTGATAGCACGCAAGTGCTGAat                                                                                                                                                                                                     <  1:568564/251‑1 (MQ=255)                                  tataCTGGATTGAATTGATTTTAGATATGCAAGAAACGCGCCGACACACTTCCCGCCGAGATTTATTATTGCAGCAAATGGGCATCAGCCAATGGCAATTGGTGCGCCCTGAAGCCTTAAAAGGCGCGATTAATATCGCCGTGCCCGAGTCGGTTCGTTTGGTTATCGTGAGCCATCAAAATCTGTTGCAACAACCCATCGCGCAGGACCTGTTGCGCGCTCTCACACTTGATAGCACGCAAGTGCTGAat                                                                                                                                                                                                     <  1:117175/251‑1 (MQ=255)                                   ataCTGGATTGAATTGATTTTAGATATGCAAGAAACGCGCCGACACACTTCCCGCCGAGATTTATTATTGCAGCAAATGGGCATCAGCCAATGGCAATTGGTGCGCCCTGAAGCCTTAAAAGGCGCGATTAATATCGCCGTGCCCGAGTCGGTTCGTTTGGTTATCGTGGGCCATCAAAATCTGTTGCAACAACCCATCGCGCAGGACCTGTTGCGCGCTCTCacac                                                                                                                                                                                                                            >  1:429445/1‑227 (MQ=255)                                   ataCTGGATTGAATTGATTTTAGATATGCAAGAAACGCGCCGACACACTTCCCGCCGAGATTTATTATTGCAGCAAATGGGCATCAGCCAATGGCAATTGGTGCGCCCTGAAGCCTTAAAAGGCGCGATTAATATCGCCGTGCCCGAGTCGGTTCGTTTGGTTATCGTGAGCCATCAAAATCTGTTGCAACAACCCATCGCGCAGGACCTGTTgcgc                                                                                                                                                                                                                                      <  1:831281/217‑1 (MQ=255)                                   ataCTGGATTGAATTGATTTTAGATATGCAAGAAACGCGCCGACACACTTCCCGCCGAGATTTATTATTGCAGCAAATGGGCATCAGCCAATGGCAATTGGTGCGCCCTGAAGCCTTAAAAGGCGCGATTAATATCGCCGTGCCCGAGTCGGTTCGTTTGGTTATCGTGAGCCATCAAAATCTGTTGCAACAACCCATCGCGCAGGACCTGTTGCGCGCTCTCACACTTGAt                                                                                                                                                                                                                       >  1:500845/1‑232 (MQ=255)                                   ataCTGGATTGAATTGATTTTAGATATGCAAGAAACGCGCCGACACACTTCCCGCCGAGATTTATTATTGCAGCAAATGGGCATCAGCCAATGGCAATTGGTGCGCCCTGAAGCCTTAAAAGGCGCGATTAATATCGCCGTGCCCGAGTCGGTTCGTTTGGTTATCGTGAGCCATCAAAATCTGTTGCAACAACCCATCGCGCAGGACCTGTTGCGCGCTCTCACACTTGATAGCACGCAAGTGCTGAata                                                                                                                                                                                                    >  1:31579/1‑251 (MQ=255)                                   ataCTGGATTGAATTGATTTTAGATATGCAAGAAACGCGCCGACACACTTCCCGCCGAGATTTATTATTGCAGCAAATGGGCATCAGCCAATGGCAATTGGTGCGCCCTGAAGCCTTAAAAGGCGCGATTAATATCGCCGTGCCCGAGTCGGTTCGTTTGGTTATCGTGAGCCATCAAAATCTGTTGCAACAACCCATCGCGCAGGACCTGTTGCGCGCTCTCACACTTGATAGCACGCAAGTGCTGAata                                                                                                                                                                                                    >  1:684253/1‑251 (MQ=255)                                   ataCTGGATTGAATTGATTTTAGATATGCAAGAAACGCGCCGACACACTTCCCGCCGAGATTTATTATTGCAGCAAATGGGCATCAGCCAATGGCAATTGGTGCGCCCTGAAGCCTTAAAAGGCGCGATTAATATCGCCGTGCCCGAGTCGGTTCGTTTGGTTATCGTGAGCCATCAAAATCTGTTGCAACAACCCATCGCGCAGGACCTGTTGCGCGCTCTCACACTTGATAGCACGCAAGTGCTGAata                                                                                                                                                                                                    >  1:402933/1‑251 (MQ=255)                                    taCTGGATTGAATTGATTTTAGATATGCAAGAAACGCGCCGACACACTTCCCGCCGAGATTTATTATTGCAGCAAATGGGCATCAGCCAATGGCAATTGGTGCGCCCTGAAGCCTTAAAAGGCGCGATTAATATCGCCGTGCCCGAGTCGGTTCGTTTGGTTATCGTGAGCCATCAAAATCTGTTGCAACAACCCATCGCGCAGGACCTGTTGCGCGCTCTCACACTTGATAGCACGCAAGTGCTGAatat                                                                                                                                                                                                   <  1:807291/251‑1 (MQ=255)                                       tGGATTGAATTGATTTTAGATATGCAAGAAACGCGCCGACCCACTTCCCGCCGAGATTTATTATTGCAGCAAATGGGTATCAGCCAATGGCAATTGGTGCGCCCTGAAGCCTTAAAAGGCGCGATTAATATCGCCGTGCCCGAGTCGGTTCGTTTGGTTATCGTGAGCCATCAAAATCTGTTGCAACAACCCATCGCGCAGGACCTGTTGCGCGCTCTCACACTTGATAGCACGCAAGTGCTGAATATCAc                                                                                                                                                                                                <  1:536250/251‑1 (MQ=255)                                        ggATTGAATTGATTTTAGATATGCAAGAAACGCGCCGACACACTTCCCGCCGAGATTTATTATTGCAGCAAATGGGCATCAGCCAATGGCAATTGGTGCGCCCTGAAGCCTTAAAAGGCGCGATTAATATCGCCGTGCCCGAGTCGGTTCGTTTGGTTATCGTGAGCCATCAAAATCTGTTGCAACAACCCATCGCGCAGGACCTGTTGCGCGCTCTCACACTTGAt                                                                                                                                                                                                                       <  1:862623/227‑1 (MQ=255)                                         gATTGAATTGATTTTAGATATGCAAGAAACGCGCCGACACACTTCCCGCCGAGATTTATTATTGCAGCAAATGGGCATCAGCCAATGGCAATTGGTGCGCCCTGAAGCCTTAAAAGGCGCGATTAATATCGCCGTGCCCGAGTCGGTTCGTTTGGTTATCGTGAGCCATCAAAATCTGTTGCAACAACCCATCGCGCAGGACCTGTTGCGCGCTCTCACACTTGATAGCACGCAAGTGCTGAATATCAcc                                                                                                                                                                                               <  1:435945/250‑1 (MQ=255)                                          attgaattgaTTTTAGATATGCAAGAAACGCGCCGACACACTTCCCGCCGAGATTTATTATTGCAGCAAATGGGCATCAGCCAATGGCAATTGGTGCGCCCTGAAGCCTTAAAAGGCGCGATTAATATCGCCGTGCCCGAGTCGGTTCGTTTGGTTATCGTGAGCCATCAAAATCTGTTGCAACAACCCATCGCGCAGGACCTGTTGCGCGCTCTCACACTTGATAGCACGCAAGTGCTGAATATCACCtt                                                                                                                                                                                             >  1:332551/1‑251 (MQ=255)                                             gaattgaTTTTAGATATGCAAGAAACGCGCCGACACACTTCCCGCCGAGATTTATTATTGCAGCAAATGGGCATCAGCCAATGGCAATTGGTGCGCCCTGAAGCCTTAAAAGGCGCGATTAATATCGCCGTGCCCGAGTCGGTTCGTTTGGTTATCGTGAGCCATCAAAATCTGTTGCAACAACCCATCGCGCAGGACCTGTTGCGCGCTCTCACACTTGATAGCACGCAAGTGCTGAATATCACCTTTGa                                                                                                                                                                                          >  1:852137/1‑251 (MQ=255)                                             gaattgaTTTTAGATATGCAAGAAACGCGCCGACACACTTCCCGCCGAGATTTATTATTGCAGCAAATGGGCATCAGCCAATGGCAATTGGTGCGCCCTGAAGCCTTAAAAGGCGCGATTAATATCGCCGTGCCCGAGTCGGTTCGTTTGGTTATCGTGAGCCATCAAAATCTGTTGCAACAACCCATCGCGCAGGACCTGTTGCGCGCTCTCACACTTGATAGCACGCAAGTGCTGAATATCACCTTTGa                                                                                                                                                                                          >  1:534470/1‑251 (MQ=255)                                             gaattgaTTTTAGATATGCAAGAAACGCGCCGACACACTTCCCGCCGAGATTTATTATTGCAGCAAATGGGCATCAGCCAATGGCAATTGGTGCGCCCTGAAGCCTTAAAAGGCGCGATTAATATCGCCGTGCCCGAGTCGGTTCGTTTGGTTATCGTGAGCCATCAAAATCTGTTGCAACAACCCATCGCGCAGGACCTGTTGCGCGCTCTCACACTTGATAGCACGCAAGTGCTGAATATCACCTTTGa                                                                                                                                                                                          >  1:122379/1‑251 (MQ=255)                                               attgaTTTTAGATATGCAAGAAACGCGCCGACACACTTCCCGCCGAGATTTATTATTGCAGCAAATGGGCATCAGCCAATGGCAATTGGTGCGCCCTGAAGCCTTAAAAGGCGCGATTAATATCGCCGTGCCCGAGTCGGTTCGTTTGGTTATCGTGAGCCATCAAAATCTGTTGCAACAACCCATCGCGCAGGACCTGTTGCGCGCTCTCACACTTGATAGCACGCAAGTGCTGAATATCACCTTTGagc                                                                                                                                                                                        <  1:808035/251‑1 (MQ=255)                                               attgaTTTTAGATATGCAAGAAACGCGCCGACACACTTCCCGCCGAGATTTATTATTGCAGCAAATGGGCATCAGCCAATGGCAATTGGTGCGCCCTGAAGCCTTAAAAGGCGCGATTAATATCGCCGTGCCCGAGTCGGTTCGTTTGGTTATCGTGAGCCATCAAAATCTGTTGCAACAACCCATCGCGCAGGACCTGTTGCGCGCTCTCACACTTGATAGCACGCAAGTGCTGAATATCACCTTTGagc                                                                                                                                                                                        <  1:832034/251‑1 (MQ=255)                                                  gaTTTTAGATATGCAAGAAACGCGCCGACACACTTCCCGCCGAGATTTATTATTGCAGCAAATGGGCATCAGCCAATGGCAATTGGTGCGCCCTGAAGCCTTAAAAGGCGCGATTAATATCGCCGTGCCCGAGTCGGTTCGTTTGGTTATCGTGAGCCATCAAAATCTGTTGCAACAACCCATCGCGCAGGACCTGTTGCGCGCTCTCACACTTGATAGCACGCAAGTGCTGAATATCACCTTTGagcag                                                                                                                                                                                      >  1:410268/1‑250 (MQ=255)                                                   aTTTTAGATATGCAAGAAACGCGCCGACACACTTCCCGCCGAGATTTATTATTGCAGCAAATGGGCATCAGCCAATGGCAATTGGTGCGCCCTGAAGCCTTAAAAGGCGCGATTAATATCGCCGTGCCCGAGTCGGTTCGTTTGGTTATCGTGAGCCATCAAAATCTGTTGCAACAACCCATCGCGCAGGACCTGTTGCGCGCTCTCACACTTGATAGCACGCAAGTGc                                                                                                                                                                                                          >  1:532736/1‑229 (MQ=255)                                                    ttttAGATATGCAAGAAACGCGCCGACACCCTTCCCGCCGAGATTTATTATTGCAGCAAATGGGCATCAGCCAATGGCAATTGGTGCGCCCTGAAGCCTTAAAAGGCGCGATTAATATCGCCGTGCCCGAGTCGGTTCGTTTGGTTATCGTGAGCCATCAAAATCTGTTGCAACAACCCATCGCGCAGGACCTGTTGCGCGCTCTCACACTTGATAGCACGCAAGTGCTGAATATCACCTTTGagcag                                                                                                                                                                                      <  1:452427/248‑1 (MQ=255)                                                                           cGACCCACTTCCCGCCGAGATTTATTATTGCAGCAAATGGGCATCAGCCAATGGCAATTGGTGCGCCCTGAAGCCTTAAAAGGCGCGATTAATATCGCCGTGCCCGAGTCGGTTCGTTTGGTTATCGTGAGCCATCAAAATCTGTTGCAACAACCCATCGCGCAGGACCTGTTGCGCGCTCTCACACTTGATAGCACGCAAGTGCTGAATATCACCTTTGAGCAGCTTGCCTATTTGCAAGTCAACCacac                                                                                                                                                            <  1:576929/251‑1 (MQ=255)                                                                              cacacTTCCCGCCGAGATTTATTATTGCAGCAAATGGGCATCAGCCAATGGCAATTGGTGCGCCCTGAAGCCTTAAAAGGCGCGATTAATATCGCCGTGCCCGAGTCGGTTCGTTTGGTTATCGTGAGCCATCAAAATCTGTTGCAACAACCCATCGCGCAGGACCTGTTGCGCGCTCTCACACTTGATAGCACGCAAGTGCTGAATATCACCTTTGagc                                                                                                                                                                                        >  1:221762/1‑220 (MQ=255)                                                                                cacTTCCCGCCGAGATTTATTATTGCAGCAAATGGGCATCAGCCAATGGCAATTGGTGCGCCCTGAAGCCTTAAAAGGCGCGATTAATATCGCCGTGCCCGAGTCGGTTCGTTTGGTTATCGTGAGCCATCAAAATCTGTTGCAACAACCCATCGCGCAGGACCTGTTGCGCGCTCTCACACTTGATAGCACGCAAGTGCTGAATATCAc                                                                                                                                                                                                <  1:629601/210‑1 (MQ=255)                                                                                         ccgAGATTTATTATTGCAGCAAATGGGCATCAGCCAATGGCAATTGGTGCGCCCTGAAGCCTTAAAAGGCGCGATTAATATCGCCGTGCCCGAGTCGGTTCGTTTGGTTATCGTGAGCCATCAAAATCTGTTGCAACAACCCATCGCGCAGGACCTGTTGCGCGCTCTCACACTTGATAGCACGCAAGTGCTGAATATCACCTTTGAGCAGCTTGCCTATTTGCAAGTCAACCACACCGTCAATTATTGGc                                                                                                                                              <  1:784097/251‑1 (MQ=255)                                                                                             gaTTTATTATTGCAGCAAATGGGCATCAGCCAATGGCAATTGGTGCGCCCTGAAGCCTTAAAAGGCGCGATTAATATCGCCGTGCCCGAGTCGGTTCGTTTGGTTATCGTGAGCCATCAAAATCTGTTGCAACAACCCATCGCGCAGGACCTGTTGCGCGCTCTCACACTTGATAGCACGCAAGTGCTGAatat                                                                                                                                                                                                   >  1:85864/1‑194 (MQ=255)                                                                                                      ttGCAGCAAATGGGCATCAGCCAATGGCAATTGGTGCGCCCTGAAGCCTTAAAAGGCGCGATTAATATCGCCGTGCCCGAGTCGGTTCGTTTGGTTATCGTGAGCCATCAAAATCTGTTGCAACAACCCATCGCGCAGGACCTGTTGCGCGCTCTCACACTTGATAGCACGCAAGTGCTGAATATCACCTTTGAGCAGCTTGCCTATTTGCAAGTCAACCACACCGTCAATTATTGGCTACTCCATaaaaa                                                                                                                                 <  1:298704/251‑1 (MQ=255)                                                                                                      ttGCAGCAAATGGGCATCAGCCAATGGCAATTGGTGCGCCCTGAAGCCTTAAAAGGCGCGATTAATATCGCCGTGCCCGAGTCGGTTCGTTTGGTTATCGTGAGCCATCAAAATCTGTTGCAACAACCCATCGCGCAGGACCTGTTGCGCGCTCTCACACTTGATAGCACGCAAGTGCTGAATATCACCTTTGAGCAGCTTGCCTATTTGCAAGTCAACCACACCGTCAATTATTGGCTACTCCATaaaaa                                                                                                                                 <  1:349097/251‑1 (MQ=255)                                                                                                        gcagcaAATGGGCATCAGCCAATGGCAATTGGTGCGCCCTGAAGCCTTAAAAGGCGCGATTAATATCGCCGTGCCCGAGTCGGTTCGTTTGGTTATCGTGAGCCATCAAAATCTGTTGCAACAACCCATCGCGCAGGACCTGTTGCGCGCTCTCACACTTGATAGCACGCAAGTGCTGAATATCACCTTTGAGCAGCTTGCCTATTTGCAAGTCAACcac                                                                                                                                                              <  1:232085/220‑1 (MQ=255)                                                                                                         cagcaAATGGGCATCAGCCAATGGCAATTGGTGCGCCCTGAAGCCTTAAAAGGCGCGATTAATATCGCCGTGCCCGAGTCGGTTCGTTTGGTTATCGTGAGCCATCAAAATCTGTTGCAACAACCCATCGCGCAGGACCTGTTGCGCGCTCTCacac                                                                                                                                                                                                                            <  1:293339/157‑1 (MQ=255)                                                                                                                  ggCATCAGCCAATGGCAATTGGTGCGCCCTGAAGCCTTAAAAGGCGCGATTAATATCGCCGTGCCCGAGTCGGTTCGTTTGGTTATCGTGAGCCATCAAAATCTGTTGCAACAACCCATCGCGCAGGACCTGTTGCGCGCTCTCACACTTGATAGCACGCAAGTGCTGAATATCACCTTTGAGCAGCTTGCCTATTTGCAAGTCAACCACACCGTCAATTATTGGCTACTCCATAAAAACGCCGACGAAAt                                                                                                                     >  1:668132/1‑251 (MQ=255)                                                                                                                         gCCAATGGCAATTGGTGCGCCCTGAAGCCTTAAAAGGCGCGATTAATATCGCCGTGCCCGAGTCGGTTCGTTTGGTTATCGTGAGCCATCAAAATCTGTTGCAACAACCCATCGCGCAGGACCTGTTGCGCGCTCTCACACTTGATAg                                                                                                                                                                                                                     >  1:727251/1‑148 (MQ=255)                                                                                                                           cAATGGCAATTGGTGCGCCCTGAAGCCTTAAAAGGCGCGATTAATATCGCCGTGCCCGAGTCGGTTCGTTTGGTTATCGTGAGCCATCAAAATCTGTTGCAACAACCCATCGCGCAGGACCTGTTGCGCGCTCTCACACTTGATAGCACGCAAGTGCTGAATATCACCTTTGAGCAGCTTGCCTATTTGCAAGTCAACCACACCGTCAatta                                                                                                                                                   <  1:438779/212‑1 (MQ=255)                                                                                                                                 cAATTGGTGCGCCCTGAAGCCTTAAAAGGCGCGATTAATATCGCCGTGCCCGAGTCGGTTCGTTTGGTTATCGTGAGCCATCAAAATCTGTTGCAACAACCCATCGCGCAGGACCTGTTGCGCGCTCTCACACTTGATAGCACGCAAGTGCTGAATATCACCTTTGAGCAGCTTGCCTATTTGCAAGTCAACCACACCGTCAATTATTGGCTACTCCATAAAAACGCCGACGAAATCCACCGCACTTTAAg                                                                                                      >  1:755473/1‑251 (MQ=255)                                                                                                                                    ttGGTGCGCCCTGAAGCCTTAAAAGGCGCGATTAATATCGCCGTGCCCGAGTCGGTTCGTTTGGTTATCGTGAGCCATCAAAATCTGTTGCAACAACCCATCGCGCAGGACCTGTTGCGCGCTCTCACACTTGATAGCACGCAAGTGCTGAATATCACCTTTGAGCAGCTTGCCTATTTGCAAGTCAACCACACCGTCAATTATTGGCTACTCCATaaaa                                                                                                                                  <  1:559576/220‑1 (MQ=255)                                                                                                                                            cccTGAAGCCTTAAAAGGCGCGATTAATATCGCCGTGCCCGAGTCGGTTCGTTTGGTTATCGTGAGCCATCAAAATCTGTTGCAACAACCCATCGCGCAGGACCTGTTGCGCGCTCTCACACTTGATAGCACGCAAGTGCTGAATATCACCTTTGAGCAGCTTGCCTATTTGCAAGTCAACCACACCGTCAATTATTGGCTACTCCATAAAAACGCCGACGAAATCCACCGCACTTTAAGCGGGCTGCaa                                                                                            >  1:321134/1‑250 (MQ=255)                                                                                                                                                   gCCTTAAAAGGCGCGATTAATATCGCCGTGCCCGAGTCGGTTCGTTTGGTTATCGTGAGCCATCAAAATCTGTTGCAACAACCCATCGCGCAGGACCTGTTGCGCGCTCTCACACTTGATAGCACGCAAGTGCTGAATATCTCCTTTGAGCAGCTTGCCTATTTGCAAGTCAACCtcac                                                                                                                                                            <  1:374220/179‑1 (MQ=255)                                                                                                                                                   gCCTTAAAAGGCGCGATTAATATCGCCGTGCCCGAGTCGGTTCGTTTGGTTATCGTGAGCCATCAAAATCTGTTGCAACAACCCATCGCGCAGGACCTGTTGCGCGCTCTCACACTTGATAGCACGCAAGTGCTGAATATCACCTTTGAGCAGCTTGCCTATTTGCAAGTCAACCACACCGTCAATTATTGGCTACTCCATAAAAACGCCGACGAAATCCACCGCACTTTAAGCGGGCTGCAACAGCCGtt                                                                                    >  1:448539/1‑251 (MQ=255)                                                                                                                                                   gCCTTAAAAGGCGCGATTAATATCGCCGTGCCCGAGTCGGTTCGTTTGGTTATCGTGAGCCATCAAAATCTGTTGCAACAACCCATCGCGCAGGACCTGTTGCGCGCTCTCACACTTGATAGCACGCAAGTGCTGAATATCACCTTTGAGCAGCTTGCCTATTTGCAAGTCAACCACACCGTCAATTATTGGCTACTCCATAAAAACGCCGACGAAATCCACCGCACTTTAAGCGGGCTGCAACAGCCGtt                                                                                    >  1:520356/1‑251 (MQ=255)                                                                                                                                                     cTTAAAAGGCGCGATTAATATCGCCGTGCCCGAGTCGGTTCGTTTGGTTATCGTGAGCCATCAAAATCTGTTGCAACAACCCATCGCGCAGGACCTGTTGCGCGCTCTCACACTTGATAGCACGCAAGTGCTGAATATCACCTTTGAGCAGCTTGCCTATTTGCAAGTCAACCACACCGTCAATTATTGGCTACTCCATAAAAACGCCGACGAAATCCACCGCACTTTAAGCGGGCTGCAACAGCCGTTGa                                                                                  >  1:817068/1‑251 (MQ=255)                                                                                                                                                                cgATTAATATCGCCGTGCCCGAGTCGGTTCGTTTGGTTATCGTGAGCCATCAAAATCTGTTGCAACAACCCATCGCGCAGGACCTGTTGCGCGCTCTCACACTTGATAGCACGCAAGTGCTGAATATCACCTTTGAGCAGCTTGCCTATTTGCAAGTCAACCACACCGTCAATTATTGGCTACTCCATAAAAACGCCGACGAAATCCACCGCACTTTAAGCGGGCTGCAACAGCCGTTGAATGTCTGGCaa                                                                       <  1:168220/251‑1 (MQ=255)                                                                                                                                                                              gtGCCCGAGTCGGTTCGTTTGGTTATCGTGAGCCATCAAAATCTGTTGCAACAACCCATCGCGCAGGACCTGTTGCGCGCTCTCACACTTGATAGCACGCAAGTGCTGAATATCACCTTTGAGCAGCTTGCCTATTTGCAAGTCAACCACACCGTCAATTATTGGCTACTCCATAAAAACGCCGACGAAATCCACCGCACTTTAAGCGGGCTGCAACAGCCGTTGAATGTCTGGCAATCCGTCGATCTcg                                                          >  1:741045/1‑250 (MQ=255)                                                                                                                                                                                          gTTCGTTTGGTTATCGTGAGCCATCAAAATCTGTTGCAACAACCCATCGCGCAGGACCTGTTGCGCGCTCTCACACTTGATAGCACGCAAGTGCTGAATATCACCTTTGAGCAGCTTGCCTATTTGCAAGTCAACCACACCGTCAATTATTGGCTACTCCATAAAAACGCCGACGAAATCCACCGCACTTTAAGCGGGCTGCAACAGCCGTTGaa                                                                                 <  1:13001/215‑1 (MQ=255)                                                                                                                                                                                           ttCGTTTGGTTATCGTGAGCCATCAAAATCTGTTGCAACAACCCATCGCGCAGGACCTGTTGCGCGCTCTCACACTTGATAGCACGCAAGTGCTGAATATCACCTTTGAGCAGCTTGCCTATTTGCAAGTCAACCACACCGTCAATTATTGGCTACTCCATAAAAACGCCGACGAAATCCACCGCACTTTAAGCGGGCTGCAACAGCCGTTGAATGTCTGGCAATCCGTCGATCTCGCCGCGTTTAAACa                                             <  1:41185/250‑1 (MQ=255)                                                                                                                                                                                           ttCGTTTGGTTATCGTGAGCCATCAAAATCTGTTGCAACAACCCATCGCGCAGGACCTGTTGCGCGCTCTCACACTTGATAGCACGCAAGTGCTGAATATCACCTTTGAGCAGCTTGCCTATTTGCAAGTCAACCACACCGTCAATTATTGGCTACTCCATAAAAACGCCGACGAAATCCACCGCACTTTAAGCGGGCTGCAACAGCCGTTGAATGTCTGGCAATCCGTCGATCTCGCCGCGTTTAAACAt                                            <  1:665475/251‑1 (MQ=255)                                                                                                                                                                                             cGTTTGGTTATCGTGAGCCATCAAAATCTGTTGCAACAACCCATCGCGCAGGACCTGTTGCGCGCTCTCACACTTGATAGCACGCAAGTGCTGAATATCACCTTTGAGCAGCTTGCCTATTTGCAAGTCAACCACACCGTCAATTATTGGCTACTCCATAAAAACGCCGACGAAATCCACCGCACTTTAAGCGGGCTGCAACAGCCGTTGAATGTCTGGCAATCCGTCGATCTCGCCGCGTTTAAACAt                                            <  1:126190/249‑1 (MQ=255)                                                                                                                                                                                                 tGGTTATCGTGAGCCATCAAAATCTGTTGCAACAACCCATCGCGCAGGACCTGTTGCGCGCTCTCACACTTGATAGCACGCAAGTGCTGAATATCACCTTTGAGCAGCTTGCCTATTTGCAAGTCAACcac                                                                                                                                                              >  1:880000/1‑131 (MQ=255)                                                                                                                                                                                                 tGGTTATCGTGAGCCATCAAAATCTGTTGCAACAACCCATCGCGCAGGACCTGTTGCGCGCTCTCACACTTGATAGCACGCAAGTGCTGAATATCACCTTTGAGCAGCTTGCCTATTTGCAAGTCAACCACACCGTCAATTATTGGCTACTCCATAAAAACGCCGACGAAATCCACCGCACTTTAAGCGGGCTGCAACAGCCGTTGAATGTCTGGCAATCCGTCGATCTCGCCGCGTTTAAACATGATCCg                                      >  1:267401/1‑251 (MQ=255)                                                                                                                                                                                                  ggTTATCGTGAGCCATCAAAATCTGTTGCAACAACCCATCGCGCAGGACCTGTTGCGCGCTCTCACACTTGATAGCACGCAAGTGCTGAATATCACCTTTGAGCAGCTTGCCTATTTGCAAGTCAACCACACCGTCAATTATTGGCTACTCCATAAAAACGCCGACGAAATCCACCGCACTTTAAGCGGGCTGCAACAGCCGTTGAATGTCTGGCAATCCGTCGATCTCGCCGCGTTTAAACATGATCCg                                      >  1:50955/1‑250 (MQ=255)                                                                                                                                                                                                                             gCAACAACCCATCGCGCAGGACCTGTTGCGCGCTCTCACACTTGATAGCACGCAAGTGCTGAATATCACCTTTGAGCAGCTTGCCTATTTGCAAGTCAACCACACCGTCAATTATTGGCTACTCCATAAAAACGCCGACGAAATCCACCGCACTTTAAGc                                                                                                     >  1:642183/1‑160 (MQ=255)                                                                                                                                                                                                                                   acccatcgcgcAGGACCTGTTGCGCGCTCTCACACTTGATAGCACGCAAGTGCTGAATATCACCTTTGAGCAGCTTGCCTATTTGCAAGTCAACCACACCGTCAATTATTGGCTACTCCATAAAAACGCCGACGAAATCCACCGCACTTTAAGCGGGCTGCAACAGCCGTTGAATGTCTGGCAATCCGTCGATCTCGCCGCGTTTAAACATGATCCGCAGGCGAAACGCCAACTCTGGCGGCAAATTCAg     >  1:251653/11‑250 (MQ=255)                                                                                                                                                                                                                                   acccatcgcgcAGGACCTGTTGCGCGCTCTCACACTTGATAGCACGCAAGTGCTGAATATCACCTTTGAGCAGCTTGCCTATTTGCAAGTCAACCACACCGTCAATTATTGGCTACTCCATAAAAACGCCGACGAAATCCACCGCACTTTAAGCGGGCTGCAACAGCCGTTGAATGTCTGGCAATCCGTCGATCTCGCCGCGTTTAAACATGATCCGCAGGCGAAACGCCAACTCTGGCGGCAAATTCAg     >  1:433296/11‑250 (MQ=255)                                                                                                                                                                                                                                    cccatcgcgcAGGACCTGTTGCGCGCTCTCACACTTGATAGCACGCAAGTGCTGAATATCACCTTTGAGCAGCTTGCCTATTTGCAAGTCAACCACACCGTCAATTATTGGCTACTCCATAAAAACGCCGACGAAATCCACCGCACTTTAAGCGGGCTGCAACAGCCGTTGAATGTCTGGCAATCCGTCGATCTCGCCGCGTTTAAACATGATCCGCAGGCGAAACGCCAACTCTGGCGGCAAATTCAGCa   >  1:161868/10‑251 (MQ=255)                                                                                                                                                                                                                                     ccatcgcgcAGGACCTGTTGCGCGCTCTCACACTTGATAGCACGCAAGTGCTGAATATCACCTTTGAGCAGCTTGCCTATTTGCAAGTCAACCACACCGTCAATTATTGGCTACTCCATAAAAACGCCGACGAAATCCACCGCACTTTAAGCGGGCTGCAACAGCCGTTGAATGTCTGGCAATCCGTCGATCTCGCCGCGTTTAAACATGATCCGCAGGCGAAACGCCAACTCTGGCGGCAAATTCAGCaa  <  1:183015/243‑1 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                              TCCTATGGCTTATTGGTTAAAATGGGCGCCCATTATACTGGATTGAATTGATTTTAGATATGCAAAAAACGCGCCGACACACTTCCCGCCGAGATTTATTATTGCAGCAAATGGGCATCAGCCAATGGCAATTGGTGCGCCCTGAAGCCTTAAAAGGCGCGATTAATATCGTCGTGCCCGAATCGGTTCGTTTGGTTATCGTGAGCCATCAAAATCTGTTGCAACAACCCATCATACAGGATCTGTTGCGCAGTCTCACACTTGATAGCACGCAAGTGCTGAATATCACCTTTGAGCAGCTTGCCTATTTGCAAGTCAACCACACCGTCAATTATTGGCTACTCCATAAAAACGCCGATGAAATCCACCGCACTTTAAGCGGGCTGCAACAGCCGTTGAATGTCTGGCAATCCGTCGATCTCGCCGCGTTTAAACATGATCCGCAGGCGAAACGCCAACTCTGGCGGCAAATTCAGCAA  >  1/1829385‑1829863

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑