Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,829,317	G→A	intergenic (+47417/+6067)	unknown → / ← unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,829,317	0	G	A	100.0%	166.2 / NA	58	intergenic (+47417/+6067)	unknown/unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (22/36);  total (22/36)

GCGGTGCATTGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGATGTTGTCTGTTGGCATAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGACGTTGTAAATCGGCGAGAAAATCATCGTTCGCCGCCCCGCTGAGCAGCAGGGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATCCTATGGCTTATTGGTTAAAATGGGCGCCCATTATACTGGATTGAATTGATTTTAGATATGCAAAAAACGCGCCGACACACTTCCCGCCGAGATTTATTATTGCAGCAAATGGGCATCAGCCAATGGCAATTGGTGCGCCCTGAAGCCTTAAAAGGCGCGATTAATATCGTCGTGCC  >  1/1829078‑1829562                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                      gcggTACATTGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCg                                                                                                                                                                                                                                              >  1:374102/1‑249 (MQ=255) gcggTACATTGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCg                                                                                                                                                                                                                                              >  1:621028/1‑249 (MQ=255)             gagagaAGCTGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCaa                                                                                                                                                                                                                                >  1:161844/1‑251 (MQ=255)                      tGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTc                                                                                                                                                                                                                       <  1:111770/251‑1 (MQ=255)                              cTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAacac                                                                                                                                                                                                                 <  1:696085/249‑1 (MQ=255)                              cTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACtt                                                                                                                                                                                                               <  1:833122/251‑1 (MQ=255)                                ggAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCg                                                                                                                                                                                                             >  1:227864/1‑251 (MQ=255)                                             cTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACaaa                                                                                                                                                                                                <  1:798096/251‑1 (MQ=255)                                              tGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAAt                                                                                                                                                                                               >  1:161682/1‑251 (MQ=255)                                              tGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAAt                                                                                                                                                                                               >  1:22942/1‑251 (MQ=255)                                                           tCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATc                                                                                                                                                                                  <  1:380476/251‑1 (MQ=255)                                                           tCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATc                                                                                                                                                                                  <  1:657975/251‑1 (MQ=255)                                                           tCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATc                                                                                                                                                                                  <  1:288961/251‑1 (MQ=255)                                                             cAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATCCt                                                                                                                                                                                <  1:117471/251‑1 (MQ=255)                                                             cAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATCCt                                                                                                                                                                                <  1:284892/251‑1 (MQ=255)                                                                 aaaaCAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGc                                                                                                                                                                                                                            <  1:790000/203‑1 (MQ=255)                                                                 aaaaCAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATCCTATgg                                                                                                                                                                            <  1:842067/251‑1 (MQ=255)                                                                  aaaCAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATCCTATGGc                                                                                                                                                                           <  1:268589/251‑1 (MQ=255)                                                                   aaCAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATCCTATGGCt                                                                                                                                                                          <  1:282305/251‑1 (MQ=255)                                                                        gATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGAc                                                                                                                                                                                                                                                    <  1:753793/172‑1 (MQ=255)                                                                                       gCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAACAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATCCTATGGc                                                                                                                                                                           >  1:442945/1‑230 (MQ=255)                                                                                          aTGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATCCTATGGCTTATTGGTTAAAATGGGCGCCCAt                                                                                                                                                   <  1:205206/251‑1 (MQ=255)                                                                                             aaaTTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATCCTATGGCTTATTGGTTAAAATGGGCGCCCATtat                                                                                                                                                <  1:325650/251‑1 (MQ=255)                                                                                               aTTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATCCTATGGCTTATTGGTTAAAATGGGCGCCCATTATAc                                                                                                                                              <  1:560422/251‑1 (MQ=255)                                                                                               aTTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATCCTATGGCTTATTGGTTAAAATGGGCGCCCATTATAc                                                                                                                                              <  1:594207/251‑1 (MQ=255)                                                                                                              ccACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACaaa                                                                                                                                                                                                >  1:481550/1‑186 (MQ=255)                                                                                                                    cACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAac                                                                                                                                                                                                                   >  1:462963/1‑161 (MQ=255)                                                                                                                    cACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATCCt                                                                                                                                                                                <  1:687501/196‑1 (MQ=255)                                                                                                                      cTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGc                                                                                                                                                                                                                            <  1:742007/150‑1 (MQ=255)                                                                                                                      cTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATCCTATGGc                                                                                                                                                                           >  1:382188/1‑199 (MQ=255)                                                                                                                        ttGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATc                                                                                                                                                                                  <  1:393692/190‑1 (MQ=255)                                                                                                                          gtgGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATCCTATGGCTTATTGGTTAAAATGGGCGCCCATTATACTg                                                                                                                                            >  1:549478/1‑226 (MQ=255)                                                                                                                               gTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATCCTATGGCTTATTGGTTAAAATGGGCGCCCATTATAc                                                                                                                                              >  1:656655/1‑219 (MQ=255)                                                                                                                                tttGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATCCTATGGCTTATTGGTTAAAATGGGCGCCCATTATACTGGATTGAATTGATTTTAGATATGCAAGAAAc                                                                                                              <  1:673120/250‑1 (MQ=255)                                                                                                                                tttGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATCCTATGGCTTATTGGTTAAAATGGGCGCCCATTATACTGGATTGAATTGATTTTAGATATGCAAGAAAc                                                                                                              <  1:503310/250‑1 (MQ=255)                                                                                                                                           cggTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATCCTATGGCTTATTgg                                                                                                                                                                    >  1:739912/3‑185 (MQ=255)                                                                                                                                           cggTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATCCTATGGCTTATTGGTTAAAATGGGCGCCCATTATACTGGATTGAATTGATTTTAGATATGCAAGAAACGCGCCGacacac                                                                                                  <  1:564288/249‑1 (MQ=255)                                                                                                                                                ggcgtAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATCCTATGGCTTATTGGTTAAAATGGGCGCCCATTATACTGGATTGAATTGATTTTAGATATGCAAGAAACGCGCCGACACACTTcc                                                                                              >  1:10092/5‑250 (MQ=255)                                                                                                                                                      gTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATCCTATGGCTTATTGGTTAAAATGGGCGCCCATTATACTGGATTGAATTGAtt                                                                                                                               >  1:598067/1‑211 (MQ=255)                                                                                                                                                        cAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATcc                                                                                                                                                                                 <  1:753925/159‑1 (MQ=255)                                                                                                                                                                  gCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATc                                                                                                                                                                                  >  1:666166/1‑148 (MQ=255)                                                                                                                                                                   cTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATc                                                                                                                                                                                  >  1:717476/1‑147 (MQ=255)                                                                                                                                                                         gcgcGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATCCTATGGCTTATTGGTTAAAATGGGCGCCCATTATAc                                                                                                                                              <  1:583273/177‑1 (MQ=255)                                                                                                                                                                              ggCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATCCTATGGCTTATTGGTTAAAATGGGCGCCCATTATACTGGATTGAATTGATTTTAGATATGCAAGAAACGCGCCGACACACTTCCCGCCGAGATTTATTATTGCAGCAAATGGGCa                                                               >  1:450767/1‑251 (MQ=255)                                                                                                                                                                                    gAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATCCTATGGCTTATTGGTTAAAATGGGCGCCCATTATACTGGATTGAATTGATTTTAGATATGCAAGAAACGCGCCGACACACTTCCCGCCGAGATTTATTATTGCAGCAAATGGGCATCAGc                                                          >  1:536005/1‑250 (MQ=255)                                                                                                                                                                                      aTCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATCCTATGGCTTATTGGTTAAAATGGGCGCCCATTATACTGGATTGAATTGATTTTAGATATGCAAGAAACGCGCCGACACACTTCCCGCCGAGATTTATTATTGCAGCAAATGGGCATCAGCCaa                                                       >  1:185177/1‑251 (MQ=255)                                                                                                                                                                                        cccTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATCCTATGGCTTATTGGTTAAAATGGGCGCCCATTATACTGGATTGAATTGATTTTAGata                                                                                                                        <  1:133275/184‑1 (MQ=255)                                                                                                                                                                                          ctggcGTTGTAAATCGGCGAGAAAATCATCATTCACCCCCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATCCTATGGCTTATTGGTTAAAATGGGCGCCCATTATACTGGATTGAATTGATTTTAGATATGCAAGAAACGCGCCGACACACTTCCCGCCGAGATTTATTATTGCAGCAAATGGGCATCAGCCAATGGc                                                   <  1:81522/247‑1 (MQ=255)                                                                                                                                                                                          ctggcGTTGTAAATCGGCGAGAAAATCATCATTCACCCCCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATCCTATGGCTTATTGGTTAAAATGGGCGCCCATTATACTGGATTGAATTGATTTTAGATATGCAAGAAACGCGCCGACACACTTCCCGCCGAGATTTATTATTGCAGCAAATGGGCATCAGCCAATGGc                                                   <  1:262236/247‑1 (MQ=255)                                                                                                                                                                                                ctGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATCCTATGGCTTATTGGTTAAAATGGGCGCCCATTATACTGGATTGAATTGATTTTAGATATGCAAGAAACGCGCCGACACACTTCCCGCCGAGATTTATTATTGCAGCAAATGGGCATCAGCCAATGGCAATTgg                                             <  1:894461/250‑1 (MQ=255)                                                                                                                                                                                                                    catcattcaccaccccGGTGAGCAGCAGAGACTTCCCGCTTAAAAAACCCAAATGCCGTTCCAAAACTTCGCTTTATGCACAAATCACGCTATTAATCCTATGGCTTATTGGTTAAAATGGGCGCCCATGATACGGGATGGAATTGTTTTTAGATAGGCAAGAAACGCGCCGACACACTTCCCGCCGAGATTTATTATTGCAGCAAATGGGCATCAGCCAATGGCAATTGGTGCGCCCTGAAGCCTCaaaa                         <  1:636808/239‑1 (MQ=255)                                                                                                                                                                                                                      tcattcaccaccccGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATCCTATGGCTTATTGGTTAAAATGGGCGCCCATTATACTGGATTGAATTGATTTTAGATATGCAAGAAACGCGCCGACACACTTCccgccg                                                                                         <  1:357649/175‑1 (MQ=255)                                                                                                                                                                                                                      tcattcaccaccccGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATCCTATGGCTTATTGGTTAAAATGGGCGCCCATTATACTGGATTGAATTGATTTTAGATATGCAAGAAACGCGCCGACACACTTCCCGCCGAGATTtattat                                                                              <  1:254010/186‑1 (MQ=255)                                                                                                                                                                                                                      tcattcaccaccccGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATCCTATGGCTTATTGGTTAAAATGGGCGCCCATTATACTGGATTGAATTGATTTTAGATATGCAAGAAACGCGCCGACACACTTCCCGCCGAGATTTATTATTGCAGCAAATGGGCATCAGCCAATGGCAATTGGTGCGCCCTGAAGCCTTAAAAgg                       <  1:83084/241‑1 (MQ=255)                                                                                                                                                                                                                                ccccGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATCCt                                                                                                                                                                                <  1:409323/88‑1 (MQ=255)                                                                                                                                                                                                                                  ccggtGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATCCTATGGCTTATTGGTTAAAATGGGCGCCCATTATACTGGATTGAATTGATTTTAGATATGCAAGAAACGCGCCGACACACTTCCCGCCGAGATTTATTATTGCAGCAAATGGGCATCAGCCAATGGCAATTGGTGCGCCCTGAAGCCTTAAAAGGCGCGATTAatat           <  1:75544/247‑1 (MQ=255)                                                                                                                                                                                                                                            gcagAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATcc                                                                                                                                                                                 >  1:356661/1‑75 (MQ=255)                                                                                                                                                                                                                                            gcagAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATCCTATGGCTTATTGGTTAAAATGGGCGCCCATTATACTGGATTGAATTGATTTTAGATATGCAAGAAACGCGCCGACACACTTCCCGCCGAGATTTATTATTGCAGCAAATGGGCATCAGCCAATGGCAATTGGTGCGCCCTGAAGCCTTAAAAGGCGCGATTAATATCGCCGTGcc  >  1:225338/1‑250 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                      GCGGTGCATTGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGATGTTGTCTGTTGGCATAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGACGTTGTAAATCGGCGAGAAAATCATCGTTCGCCGCCCCGCTGAGCAGCAGGGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATCCTATGGCTTATTGGTTAAAATGGGCGCCCATTATACTGGATTGAATTGATTTTAGATATGCAAAAAACGCGCCGACACACTTCCCGCCGAGATTTATTATTGCAGCAAATGGGCATCAGCCAATGGCAATTGGTGCGCCCTGAAGCCTTAAAAGGCGCGATTAATATCGTCGTGCC  >  1/1829078‑1829562

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑