Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,829,266	A→G	intergenic (+47366/+6118)	unknown → / ← unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,829,266	0	A	G	100.0%	170.0 / NA	58	intergenic (+47366/+6118)	unknown/unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/33);  total (25/33)

CCGCTACGATTTTCGCCCACCAGCAAAACCTCCTGCCCGGCGGTGCATTGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGATGTTGTCTGTTGGCATAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGACGTTGTAAATCGGCGAGAAAATCATCGTTCGCCGCCCCGCTGAGCAGCAGGGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATCCTATGGCTTATTGGTTAAAATGGGCGCCCATTATACTGGATTGAATTGATTTTAGATATGCAAAAAACGCGCCGACACACTTCCCGCCGAGATTTATTATTGCAGCAAATGGGCATCAGCCAA  >  1/1829039‑1829509                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                    ccacTACGATTTTCGCCCACCAGTAAAACTTCCTGTCCGGCGGTACATTGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAat                                                                                                                                                                                                                               >  1:800593/4‑250 (MQ=255)           tttCGCCCACCAGTAAAACTTCCTGTCCGGCGGTACATTGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTcacc                                                                                                                                                                                                                    <  1:86709/251‑4 (MQ=255)                    ccaGTAAAACTTCCTGTCCGGCGGTACATTGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCAcccc                                                                                                                                                                                                               >  1:748261/1‑247 (MQ=255)                     cagtaaaaCTTCCTGTCCGGCGGTACATTGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGa                                                                                                                                                                                                          >  1:70827/5‑251 (MQ=255)                       gtaaaaCTTCCTGTCCGGCGGTACATTGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGa                                                                                                                                                                                                          >  1:69385/3‑249 (MQ=255)                                        gcggTACATTGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCg                                                                                                                                                                                         >  1:621028/1‑249 (MQ=255)                                        gcggTACATTGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCg                                                                                                                                                                                         >  1:374102/1‑249 (MQ=255)                                               aTTGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTcaccaccc                                                                                                                                                                                                                <  1:866651/219‑8 (MQ=255)                                                ttGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGa                                                                                                                                                                                                          <  1:53771/224‑1 (MQ=255)                                                    gagagaAGCTGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCaa                                                                                                                                                                           >  1:161844/1‑251 (MQ=255)                                                        gaAGCTGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCgg                                                                                                                                                                                                             <  1:500441/213‑2 (MQ=255)                                                             tGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTc                                                                                                                                                                  <  1:111770/251‑1 (MQ=255)                                                                     cTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAacac                                                                                                                                                            <  1:696085/249‑1 (MQ=255)                                                                     cTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACtt                                                                                                                                                          <  1:833122/251‑1 (MQ=255)                                                                       ggAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCg                                                                                                                                                        >  1:227864/1‑251 (MQ=255)                                                                        gAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCgg                                                                                                                                                                                                                                        <  1:297107/170‑1 (MQ=255)                                                                                    cTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACaaa                                                                                                                                           <  1:798096/251‑1 (MQ=255)                                                                                     tGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAAt                                                                                                                                          >  1:161682/1‑251 (MQ=255)                                                                                     tGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAAt                                                                                                                                          >  1:22942/1‑251 (MQ=255)                                                                                      gTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGcagcagag                                                                                                                                                                                                 <  1:17806/195‑3 (MQ=255)                                                                                                  tCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATc                                                                                                                             <  1:288961/251‑1 (MQ=255)                                                                                                  tCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATc                                                                                                                             <  1:380476/251‑1 (MQ=255)                                                                                                  tCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATc                                                                                                                             <  1:657975/251‑1 (MQ=255)                                                                                                    cAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATCCt                                                                                                                           <  1:284892/251‑1 (MQ=255)                                                                                                    cAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATCCt                                                                                                                           <  1:117471/251‑1 (MQ=255)                                                                                                        aaaaCAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGc                                                                                                                                                                       <  1:790000/203‑1 (MQ=255)                                                                                                        aaaaCAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATCCTATgg                                                                                                                       <  1:842067/251‑1 (MQ=255)                                                                                                         aaaCAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATCCTATGGc                                                                                                                      <  1:268589/251‑1 (MQ=255)                                                                                                          aaCAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATCCTATGGCt                                                                                                                     <  1:282305/251‑1 (MQ=255)                                                                                                               gATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGAc                                                                                                                                                                                               <  1:753793/172‑1 (MQ=255)                                                                                                                aTCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCgg                                                                                                                                                                                                             >  1:102828/1‑156 (MQ=255)                                                                                                                              gCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAACAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATCCTATGGc                                                                                                                      >  1:442945/1‑230 (MQ=255)                                                                                                                                 aTGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATCCTATGGCTTATTGGTTAAAATGGGCGCCCAt                                                                                              <  1:205206/251‑1 (MQ=255)                                                                                                                                    aaaTTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATCCTATGGCTTATTGGTTAAAATGGGCGCCCATtat                                                                                           <  1:325650/251‑1 (MQ=255)                                                                                                                                      aTTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATCCTATGGCTTATTGGTTAAAATGGGCGCCCATTATAc                                                                                         <  1:594207/251‑1 (MQ=255)                                                                                                                                      aTTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATCCTATGGCTTATTGGTTAAAATGGGCGCCCATTATAc                                                                                         <  1:560422/251‑1 (MQ=255)                                                                                                                                                     ccACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACaaa                                                                                                                                           >  1:481550/1‑186 (MQ=255)                                                                                                                                                           cACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAac                                                                                                                                                              >  1:462963/1‑161 (MQ=255)                                                                                                                                                           cACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATCCt                                                                                                                           <  1:687501/196‑1 (MQ=255)                                                                                                                                                             cTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGc                                                                                                                                                                       <  1:742007/150‑1 (MQ=255)                                                                                                                                                             cTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATCCTATGGc                                                                                                                      >  1:382188/1‑199 (MQ=255)                                                                                                                                                               ttGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATc                                                                                                                             <  1:393692/190‑1 (MQ=255)                                                                                                                                                                 gtgGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATCCTATGGCTTATTGGTTAAAATGGGCGCCCATTATACTg                                                                                       >  1:549478/1‑226 (MQ=255)                                                                                                                                                                      gTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATCCTATGGCTTATTGGTTAAAATGGGCGCCCATTATAc                                                                                         >  1:656655/1‑219 (MQ=255)                                                                                                                                                                       tttGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATCCTATGGCTTATTGGTTAAAATGGGCGCCCATTATACTGGATTGAATTGATTTTAGATATGCAAGAAAc                                                         <  1:673120/250‑1 (MQ=255)                                                                                                                                                                       tttGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATCCTATGGCTTATTGGTTAAAATGGGCGCCCATTATACTGGATTGAATTGATTTTAGATATGCAAGAAAc                                                         <  1:503310/250‑1 (MQ=255)                                                                                                                                                                                  cggTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATCCTATGGCTTATTgg                                                                                                               >  1:739912/3‑185 (MQ=255)                                                                                                                                                                                  cggTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATCCTATGGCTTATTGGTTAAAATGGGCGCCCATTATACTGGATTGAATTGATTTTAGATATGCAAGAAACGCGCCGacacac                                             <  1:564288/249‑1 (MQ=255)                                                                                                                                                                                       ggcgtAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATCCTATGGCTTATTGGTTAAAATGGGCGCCCATTATACTGGATTGAATTGATTTTAGATATGCAAGAAACGCGCCGACACACTTcc                                         >  1:10092/5‑250 (MQ=255)                                                                                                                                                                                             gTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATCCTATGGCTTATTGGTTAAAATGGGCGCCCATTATACTGGATTGAATTGAtt                                                                          >  1:598067/1‑211 (MQ=255)                                                                                                                                                                                               cAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATcc                                                                                                                            <  1:753925/159‑1 (MQ=255)                                                                                                                                                                                                         gCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATc                                                                                                                             >  1:666166/1‑148 (MQ=255)                                                                                                                                                                                                          cTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATc                                                                                                                             >  1:717476/1‑147 (MQ=255)                                                                                                                                                                                                                gcgcGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATCCTATGGCTTATTGGTTAAAATGGGCGCCCATTATAc                                                                                         <  1:583273/177‑1 (MQ=255)                                                                                                                                                                                                                     ggCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATCCTATGGCTTATTGGTTAAAATGGGCGCCCATTATACTGGATTGAATTGATTTTAGATATGCAAGAAACGCGCCGACACACTTCCCGCCGAGATTTATTATTGCAGCAAATGGGCa          >  1:450767/1‑251 (MQ=255)                                                                                                                                                                                                                           gAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATCCTATGGCTTATTGGTTAAAATGGGCGCCCATTATACTGGATTGAATTGATTTTAGATATGCAAGAAACGCGCCGACACACTTCCCGCCGAGATTTATTATTGCAGCAAATGGGCATCAGc     >  1:536005/1‑250 (MQ=255)                                                                                                                                                                                                                             aTCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATCCTATGGCTTATTGGTTAAAATGGGCGCCCATTATACTGGATTGAATTGATTTTAGATATGCAAGAAACGCGCCGACACACTTCCCGCCGAGATTTATTATTGCAGCAAATGGGCATCAGCCaa  >  1:185177/1‑251 (MQ=255)                                                                                                                                                                                                                               cccTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATCCTATGGCTTATTGGTTAAAATGGGCGCCCATTATACTGGATTGAATTGATTTTAGata                                                                   <  1:133275/184‑1 (MQ=255)                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                    CCGCTACGATTTTCGCCCACCAGCAAAACCTCCTGCCCGGCGGTGCATTGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGATGTTGTCTGTTGGCATAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGACGTTGTAAATCGGCGAGAAAATCATCGTTCGCCGCCCCGCTGAGCAGCAGGGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATCCTATGGCTTATTGGTTAAAATGGGCGCCCATTATACTGGATTGAATTGATTTTAGATATGCAAAAAACGCGCCGACACACTTCCCGCCGAGATTTATTATTGCAGCAAATGGGCATCAGCCAA  >  1/1829039‑1829509

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑