Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,829,209	A→G	intergenic (+47309/+6175)	unknown → / ← unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,829,209	0	A	G	100.0%	156.5 / NA	55	intergenic (+47309/+6175)	unknown/unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (20/35);  total (20/35)

GCAACGGCGGGCGGAGTCGATTTTGTGAATATCGCCGTAATCGGCAAGCATTTTTTCGGCGGAACGCACGCCGCTACGATTTTCGCCCACCAGCAAAACCTCCTGCCCGGCGGTGCATTGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGATGTTGTCTGTTGGCATAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGACGTTGTAAATCGGCGAGAAAATCATCGTTCGCCGCCCCGCTGAGCAGCAGGGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATCCTATGGCTTATTGGTTAAAATGGGCGCCCATTATACTGGATTGAATTGATTTTAGATATGCAAAAAAC  >  1/1828969‑1829454                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                      gCAACGACGGGCGGAGTCGATTTTGTGAATATCGCCGTAATCGGCAAGCATTTTTTCGGCGGAACGCACACCACTACGATTTTCGCCCACCAGTAAAACTTCCTGTCCGGCGGTACATTGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTcggt                                                                                                                                                                                                                                             >  1:369840/1‑248 (MQ=255)            cggAGTCGATTTTGTGAATATCGCCGTAATCGGCAAGCATTTTTTCGGCGGAACGCACACCACTACGATTTTCGCCCACCAGTAAAACTTCCTGTCCGGCGGTACATTGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTc                                                                                                                                                                                                                                   <  1:381376/250‑1 (MQ=255)                  cGATTTTGTGAATATCGCCGTAATCGGCAAGCATTTTTTCGGCGGAACGCACACCACTACGATTTTCGCCCACCAGTAAAACTTCCTGTCCGGCGGTACATTGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATa                                                                                                                                                                                                                             >  1:871367/1‑250 (MQ=255)                  cGATTTTGTGAATATCGCCGTAATCGGCAAGCATTTTTTCGGCGGAACGCACACCACTACGATTTTCGCCCACCAGTAAAACTTCCTGTCCGGCGGTACATTGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATAc                                                                                                                                                                                                                            <  1:786551/251‑1 (MQ=255)                              tatCGCCGTAATCGGCAAGCATTTTTTCGGCGGAACGCACACCACTACGATTTTCGCCCACCAGTAAAACTTCCTGTCCGGCGGTACATTGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATgcg                                                                                                                                                                                                                <  1:220115/251‑1 (MQ=255)                                     gTAATCGGCAAGCATTTTTTCGGCGGAACGCACACCACTACGATTTTCGCCCACCAGTAAAACTTCCTGTCCGGCGGTACATTGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGt                                                                                                                                                                                                         >  1:272452/1‑251 (MQ=255)                                                 cATTTTTTCGGCGGAACGCACACCACTACGATTTTCGCCCACCAGTAAAACTTCCTGTCCGGCGGTACATTGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTggc                                                                                                                                                                                             <  1:516195/251‑3 (MQ=255)                                                              gAACGCACACCACTACGATTTTCGCCCACCAGTAAAACTTCCTGTCCGGCGGTACATTGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTc                                                                                                                                                                                                                      <  1:688054/213‑1 (MQ=255)                                                                       ccacTACGATTTTCGCCCACCAGTAAAACTTCCTGTCCGGCGGTACATTGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAat                                                                                                                                                                        >  1:800593/4‑250 (MQ=255)                                                                          cTACGATTTTCGCCCACCAGTAAAACTTCCTGTCCGGCGGTACATTGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTc                                                                                                                                                                                                                                   <  1:506759/188‑1 (MQ=255)                                                                                 tttCGCCCACCAGTAAAACTTCCTGTCCGGCGGTACATTGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTcacc                                                                                                                                                             <  1:86709/251‑4 (MQ=255)                                                                                          ccaGTAAAACTTCCTGTCCGGCGGTACATTGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCAcccc                                                                                                                                                        >  1:748261/1‑247 (MQ=255)                                                                                           cagtaaaaCTTCCTGTCCGGCGGTACATTGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGa                                                                                                                                                   >  1:70827/5‑251 (MQ=255)                                                                                             gtaaaaCTTCCTGTCCGGCGGTACATTGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGa                                                                                                                                                   >  1:69385/3‑249 (MQ=255)                                                                                                              gcggTACATTGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCg                                                                                                                                  >  1:621028/1‑249 (MQ=255)                                                                                                              gcggTACATTGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCg                                                                                                                                  >  1:374102/1‑249 (MQ=255)                                                                                                                     aTTGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTcaccaccc                                                                                                                                                         <  1:866651/219‑8 (MQ=255)                                                                                                                      ttGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGa                                                                                                                                                   <  1:53771/224‑1 (MQ=255)                                                                                                                          gagagaAGCTGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCaa                                                                                                                    >  1:161844/1‑251 (MQ=255)                                                                                                                              gaAGCTGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCgg                                                                                                                                                      <  1:500441/213‑2 (MQ=255)                                                                                                                                   tGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTc                                                                                                           <  1:111770/251‑1 (MQ=255)                                                                                                                                           cTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAacac                                                                                                     <  1:696085/249‑1 (MQ=255)                                                                                                                                           cTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACtt                                                                                                   <  1:833122/251‑1 (MQ=255)                                                                                                                                             ggAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCg                                                                                                 >  1:227864/1‑251 (MQ=255)                                                                                                                                              gAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCgg                                                                                                                                                                                 <  1:297107/170‑1 (MQ=255)                                                                                                                                                          cTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACaaa                                                                                    <  1:798096/251‑1 (MQ=255)                                                                                                                                                           tGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAAt                                                                                   >  1:22942/1‑251 (MQ=255)                                                                                                                                                           tGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAAt                                                                                   >  1:161682/1‑251 (MQ=255)                                                                                                                                                            gTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGcagcagag                                                                                                                                          <  1:17806/195‑3 (MQ=255)                                                                                                                                                                        tCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATc                                                                      <  1:380476/251‑1 (MQ=255)                                                                                                                                                                        tCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATc                                                                      <  1:288961/251‑1 (MQ=255)                                                                                                                                                                        tCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATc                                                                      <  1:657975/251‑1 (MQ=255)                                                                                                                                                                          cAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATCCt                                                                    <  1:117471/251‑1 (MQ=255)                                                                                                                                                                          cAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATCCt                                                                    <  1:284892/251‑1 (MQ=255)                                                                                                                                                                              aaaaCAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGc                                                                                                                <  1:790000/203‑1 (MQ=255)                                                                                                                                                                              aaaaCAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATCCTATgg                                                                <  1:842067/251‑1 (MQ=255)                                                                                                                                                                               aaaCAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATCCTATGGc                                                               <  1:268589/251‑1 (MQ=255)                                                                                                                                                                                aaCAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATCCTATGGCt                                                              <  1:282305/251‑1 (MQ=255)                                                                                                                                                                                     gATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGAc                                                                                                                                        <  1:753793/172‑1 (MQ=255)                                                                                                                                                                                      aTCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCgg                                                                                                                                                      >  1:102828/1‑156 (MQ=255)                                                                                                                                                                                                    gCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAACAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATCCTATGGc                                                               >  1:442945/1‑230 (MQ=255)                                                                                                                                                                                                       aTGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATCCTATGGCTTATTGGTTAAAATGGGCGCCCAt                                       <  1:205206/251‑1 (MQ=255)                                                                                                                                                                                                          aaaTTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATCCTATGGCTTATTGGTTAAAATGGGCGCCCATtat                                    <  1:325650/251‑1 (MQ=255)                                                                                                                                                                                                            aTTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATCCTATGGCTTATTGGTTAAAATGGGCGCCCATTATAc                                  <  1:594207/251‑1 (MQ=255)                                                                                                                                                                                                            aTTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATCCTATGGCTTATTGGTTAAAATGGGCGCCCATTATAc                                  <  1:560422/251‑1 (MQ=255)                                                                                                                                                                                                                           ccACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACaaa                                                                                    >  1:481550/1‑186 (MQ=255)                                                                                                                                                                                                                                 cACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAac                                                                                                       >  1:462963/1‑161 (MQ=255)                                                                                                                                                                                                                                 cACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATCCt                                                                    <  1:687501/196‑1 (MQ=255)                                                                                                                                                                                                                                   cTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGc                                                                                                                <  1:742007/150‑1 (MQ=255)                                                                                                                                                                                                                                   cTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATCCTATGGc                                                               >  1:382188/1‑199 (MQ=255)                                                                                                                                                                                                                                     ttGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATc                                                                      <  1:393692/190‑1 (MQ=255)                                                                                                                                                                                                                                       gtgGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATCCTATGGCTTATTGGTTAAAATGGGCGCCCATTATACTg                                >  1:549478/1‑226 (MQ=255)                                                                                                                                                                                                                                            gTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATCCTATGGCTTATTGGTTAAAATGGGCGCCCATTATAc                                  >  1:656655/1‑219 (MQ=255)                                                                                                                                                                                                                                             tttGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATCCTATGGCTTATTGGTTAAAATGGGCGCCCATTATACTGGATTGAATTGATTTTAGATATGCAAGAAAc  <  1:673120/250‑1 (MQ=255)                                                                                                                                                                                                                                             tttGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATCCTATGGCTTATTGGTTAAAATGGGCGCCCATTATACTGGATTGAATTGATTTTAGATATGCAAGAAAc  <  1:503310/250‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                      GCAACGGCGGGCGGAGTCGATTTTGTGAATATCGCCGTAATCGGCAAGCATTTTTTCGGCGGAACGCACGCCGCTACGATTTTCGCCCACCAGCAAAACCTCCTGCCCGGCGGTGCATTGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGATGTTGTCTGTTGGCATAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGACGTTGTAAATCGGCGAGAAAATCATCGTTCGCCGCCCCGCTGAGCAGCAGGGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATCCTATGGCTTATTGGTTAAAATGGGCGCCCATTATACTGGATTGAATTGATTTTAGATATGCAAAAAAC  >  1/1828969‑1829454

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑