Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,829,083	G→A	intergenic (+47183/+6301)	unknown → / ← unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,829,083	0	G	A	100.0%	161.3 / NA	55	intergenic (+47183/+6301)	unknown/unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (32/23);  total (32/23)

CCAGTTCGGCGGCGCTGAATACGCCGGGCAACGCATAGATAGTTAAATCGGCAATTTGTGGCGATTGATAGGATTTCCAGAAAGTTTTCAGTTCAAAGTGCGGTGGATTTTTTAACTGAAAATGATACAGACTGCAACGGCGGGCGGAGTCGATTTTGTGAATATCGCCGTAATCGGCAAGCATTTTTTCGGCGGAACGCACGCCGCTACGATTTTCGCCCACCAGCAAAACCTCCTGCCCGGCGGTGCATTGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGATGTTGTCTGTTGGCATAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGACGTTGTAAATCGGCGAGAAAATCATCGTTCGCCGCCCCGCTGAGCAGCAGGGACTTCCCG  >  1/1828836‑1829326                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                    ccAGCTCGGCGGCGCTGAATACGCCGGGCAACGCATAGATAGTTAAATCGGCAAGTTGCGACGATTGATAGGATTTCCAGAAAGTTTTCAGTTCAAAGTGCGGTGGATTTTTTAATTGAAAATGATACAGACTGCAACGACGGGCGGAGTCGATTTTGTGAATATCGCCGTAATCGGCAAGCATTTTTTCGGCGGAACGCACACCACTACGATTTTCGCCCACCAGTAAAACTTCCTGTCCGGCGGTACAt                                                                                                                                                                                                                                                  <  1:899841/251‑1 (MQ=255) ccAGCTCGGCGGCGCTGAATACGCCGGGCAACGCATAGATAGTTAAATCGGCAAGTTGCGACGATTGATAGGATTTCCAGAAAGTTTTCAGTTCAAAGTGCGGTGGATTTTTTAATTGAAAATGATACAGACTGCAACGACGGGCGGAGTCGATTTTGTGAATATCGCCGTAATCGGCAAGCATTTTTTCGGCGGAACGCACACCACTACGATTTTCGCCCACCAGTAAAACTTCCTGTCCGGCGGTACAt                                                                                                                                                                                                                                                  <  1:452063/251‑1 (MQ=255) ccAGCTCGGCGGCGCTGAATACGCCGGGCAACGCATAGATAGTTAAATCGGCAAGTTGCGACGATTGATAGGATTTCCAGAAAGTTTTCAGTTCAAAGTGCGGTGGATTTTTTAATTGAAAATGATACAGACTGCAACGACGGGCGGAGTCGATTTTGTGAATATCGCCGTAATCGGCAAGCATTTTTTCGGCGGAACGCACACCACTACGATTTTCGCCCACCAGTAAAACTTCCTGTCCGGCGGTACAt                                                                                                                                                                                                                                                  <  1:385221/251‑1 (MQ=255)               cTGAATACGCCGGGCAACGCATAGATAGTTAAATCGGCAAGTTGCGACGATTGATAGGATTTCCAGAAAGTTTTCAGTTCAAAGTGCGGTGGATTTTTTAATTGAAAATGATACAGACTGCAACGACGGGCGGAGTCGATTTTGTGAATATCGCCGTAATCGGCAAGCATTTTTTCGGCGGAACGCACACCACTACGATTTTCGCCCACCAGTAAAACTTCCTGTCCGGCGGTACATTGTGAGAGAAg                                                                                                                                                                                                                                       >  1:150425/1‑248 (MQ=255)                 gAATACGCCGGGCAACGCATAGATAGTTAAATCGGCAAGTTGCGACGATTGATAGGATTTCCAGAAAGTTTTCAGTTCAAAGTGCGGTGGATTTTTTAATTGAAAATGATACAGACTGCAACGACGGGCGGAGTCGATTTTGTGAATATCGCCGTAATCGGCAAGCATTTTTTCGGCGGAACGCACACCACTACGATTTTCGCCCACCAGTAAAACTTCCTGTCCGGCGGTACAt                                                                                                                                                                                                                                                  <  1:599727/235‑1 (MQ=255)                 gAATACGCCGGGCAACGCATAGATAGTTAAATCGGCAAGTTGCGACGATTGATAGGATTTCCAGAAAGTTTTCAGTTCAAAGTGCGGTGGATTTTTTAATTGAAAATGATACAGACTGCAACGACGGGCGGAGTCGATTTTGTGAATATCGCCGTAATCGGCAAGCATTTTTTCGGCGGAACGCACACCACTACGATTTTCGCCCACCAGTAAAACTTCCTGTCCGGCGGTACATTGTGAGAGAAGCTGCa                                                                                                                                                                                                                                  >  1:110471/1‑251 (MQ=255)                 gAATACGCCGGGCAACGCATAGATAGTTAAATCGGCAAGTTGCGACGATTGATAGGATTTCCAGAAAGTTTTCAGTTCAAAGTGCGGTGGATTTTTTAATTGAAAATGATACAGACTGCAACGACGGGCGGAGTCGATTTTGTGAATATCGCCGTAATCGGCAAGCATTTTTTCGGCGGAACGCACACCACTACGATTTTCGCCCACCAGTAAAACTTCCTGTCCGGCGGTACATTGTGAGAGAAGCTGCa                                                                                                                                                                                                                                  >  1:194344/1‑251 (MQ=255)                 gAATACGCCGGGCAACGCATAGATAGTTAAATCGGCAAGTTGCGACGATTGATAGGATTTCCAGAAAGTTTTCAGTTCAAAGTGCGGTGGATTTTTTAATTGAAAATGATACAGACTGCAACGACGGGCGGAGTCGATTTTGTGAATATCGCCGTAATCGGCAAGCATTTTTTCGGCGGAACGCACACCACTACGATTTTCGCCCACCAGTAAAACTTCCTGTCCGGCGGTACATTGTGAGAGAAGCTGCa                                                                                                                                                                                                                                  >  1:699636/1‑251 (MQ=255)                            gCAACGCATAGATAGTTAAATCGGCAAGTTGCGACGATTGATAGGATTTCCAGAAAGTTTTCAGTTCAAAGTGCGGTGGATTTTTTAATTGAAAATGATACAGACTGCAACGACGGGCGGAGTCGATTTTGTGAATATCGCCGTAATCGGCAAGCATTTTTTCGGCGGAACGCACACCACTACGATTTTCGCCCACCAGTAAAACTTCCTGTCCGGCGGTACATTGTgag                                                                                                                                                                                                                                            <  1:275620/230‑1 (MQ=255)                               aCGCATAGATAGTTAAATCGGCAAGTTGCGACGATTGATAGGATTTCCAGAAAGTTTTCAGTTCAAAGTGCGGTGGATTTTTTAATTGAAAATGATACAGACTGCAACGACGGGCGGAGTCGATTTTGTGAATATCGCCGTAATCGGCAAGCATTTTTTCGGCGGAACGCACACCACTACGATTTTCGCCCACCAGTAAAACTTCCTGTCCGGCGGTACATTGTGAGAGAAGCTGCATGAGCTGGAAATgg                                                                                                                                                                                                                    >  1:99561/1‑251 (MQ=255)                                cGCATAGATAGTTAAATCGGCAAGTTGCGACGATTGATAGGATTTCCAGAAAGTTTTCAGTTCAAAGTGCGGTGGATTTTTTAATTGAAAATGATACAGACTGCAACGACGGGCGGAGTCGATTTTGTGAATATCGCCGTAATCGGCAAGCATTTTTTCGGCGGAACGCACACCACTACGATTTTCGCCCACCAGTAAAACTTCCTGTCCGGCGGTACATTGTGAGAGAAGCTGCATGAGCTGGAAATgg                                                                                                                                                                                                                    >  1:212149/1‑250 (MQ=255)                                cGCATAGATAGTTAAATCGGCAAGTTGCGACGATTGATAGGATTTCCAGAAAGTTTTCAGTTCAAAGTGCGGTGGATTTTTTAATTGAAAATGATACAGACTGCAACGACGGGCGGAGTCGATTTTGTGAATATCGCCGTAATCGGCAAGCATTTTTTCGGCGGAACGCACACCACTACGATTTTCGCCCACCAGTAAAACTTCCTGTCCGGCGGTACATTGTGAGAGAAGCTGCATGAGCTGGAAATgg                                                                                                                                                                                                                    >  1:845888/1‑250 (MQ=255)                                cGCATAGATAGTTAAATCGGCAAGTTGCGACGATTGATAGGATTTCCAGAAAGTTTTCAGTTCAAAGTGCGGTGGATTTTTTAATTGAAAATGATACAGACTGCAACGACGGGCGGAGTCGATTTTGTGAATATCGCCGTAATCGGCAAGCATTTTTTCGGCGGAACGCACACCACTACGATTTTCGCCCACCAGTAAAACTTCCTGTCCGGCGGTACATTGTGAGAGAAGCTGCATGAGCTGGAAATgg                                                                                                                                                                                                                    >  1:749738/1‑250 (MQ=255)                                 gCATAGATAGTTAAATCGGCAAGTTGCGACGATTGATAGGATTTCCAGAAAGTTTTCAGTTCAAAGTGCGGTGGATTTTTTAATTGAAAATGATACAGACTGCAACGACGGGCGGAGTCGATTTTGTGAATATCGCCGTAATCGGCAAGCATTTTTTCGGCGGAACGCACACCACTACGATTTTCGCCCACCAGTAAAACTTCCTGTCCGGCGGTACATTGTGAGAGAAGCTGCATGAGCTGGAAATGGCa                                                                                                                                                                                                                  >  1:169108/1‑251 (MQ=255)                                        tagTTAAATCGGCAAGTTGCGACGATTGATAGGATTTCCAGAAAGTTTTCAGTTCAAAGTGCGGTGGATTTTTTAATTGAAAATGATACAGACTGCAACGACGGGCGGAGTCGATTTTGTGAATATCGCCGTAATCGGCAAGCATTTTTTCGGCGGAACGCACACCACTACGATTTTCGCCCACCAGTAAAACTTCCTGTCCGGCGGTACATTGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTc                                                                                                                                                                                                               >  1:529807/1‑247 (MQ=255)                                        tagTTAAATCGGCAAGTTGCGACGATTGATAGGATTTCCAGAAAGTTTTCAGTTCAAAGTGCGGTGGATTTTTTAATTGAAAATGATACAGACTGCAACGACGGGCGGAGTCGATTTTGTGAATATCGCCGTAATCGGCAAGCATTTTTTCGGCGGAACGCACACCACTACGATTTTCGCCCACCAGTAAAACTTCCTGTCCGGCGGTACATTGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTc                                                                                                                                                                                                               >  1:783633/1‑247 (MQ=255)                                              aaTCGGCAAGTTGCGACGATTGATAGGATTTCCAGAAAGTTTTCAGTTCAAAGTGCGGTGGATTTTTTAATTGAAAATGATACAGACTGCAACGACGGGCGGAGTCGATTTTGTGAATATCGCCGTAATCGGCAAGCATTTTTTCGGCGGAACGCACACCACTACGATTTTCGCCCACCAGTAAAACTTCCTGTCCGGCGGTACATTGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTCCTGTTTAttc                                                                                                                                                                                                     <  1:244789/251‑1 (MQ=255)                                                               gATTGATAGGATTTCCAGAAAGTTTTCAGTTCAAAGTGCGGTGGATTTTTTAATTGAAAATGATACAGACTGCAACGACGGGCGGAGTCGATTTTGTGAATATCGCCGTAATCGGCAAGCATTTTTTCGGCGGAACGCACACCACTACGATTTTCGCCCACCAGTAAAACTTCCTGTCCGGCGGTACAt                                                                                                                                                                                                                                                  >  1:34984/1‑189 (MQ=255)                                                               gATTGATAGGATTTCCAGAAAGTTTTCAGTTCAAAGTGCGGTGGATTTTTTAATTGAAAATGATACAGACTGCAACGACGGGCGGAGTCGATTTTGTGAATATCGCCGTAATCGGCAAGCATTTTTTCGGCGGAACGCACACCACTACGATTTTCGCCCACCAGTAAAACTTCCTGTCCGGCGGTACATTGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAg                                                                                                                                                                                    >  1:381789/1‑251 (MQ=255)                                                                     tAGGATTTCCAGAAAGTTTTCAGTTCAAAGTGCGGTGGATTTTTTAATTGAAAATGATACAGACTGCAACGACGGGCGGAGTCGATTTTGTGAATATCGCCGTAATCGGCAAGCATTTTTTCGGCGGAACGCACACCACTACGATTTTCGCCCACCAGTAAAACTTCCTGTCCGGCGGTACATTGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATcac                                                                                                                                                                              <  1:193855/251‑1 (MQ=255)                                                                         aTTTCCAGAAAGTTTTCAGTTCAAAGTGCGGTGGATTTTTTAATTGAAAATGATACAGACTGCAACGACGGGCGGAGTCGATTTTGTGAATATCGCCGTAATCGGCAAGCATTTTTTCGGCGGAACGCACACCACTACGATTTTCGCCCACCAGTAAAACTTCCTGTCCGGCGGTACATTGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATc                                                                                                                                                                           <  1:6723/250‑1 (MQ=255)                                                                         aTTTCCAGAAAGTTTTCAGTTCAAAGTGCGGTGGATTTTTTAATTGAAAATGATACAGACTGCAACGACGGGCGGAGTCGATTTTGTGAATATCGCCGTAATCGGCAAGCATTTTTTCGGCGGAACGCACACCACTACGATTTTCGCCCACCAGTAAAACTTCCTGTCCGGCGGTACATTGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCg                                                                                                                                                                          <  1:688114/251‑1 (MQ=255)                                                                            tCCAGAAAGTTTTCAGTTCAAAGTGCGGTGGATTTTTTAATTGAAAATGATACAGACTGCAACGACGGGCGGAGTCGATTTTGTGAATATCGCCGTAATCGGCAAGCATTTTTTCGGCGGAACGCACACCACTACGATTTTCGCCCACCAGTAAAACTTCCTGTCCGGCGGTACATTGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGgcg                                                                                                                                                                       <  1:794608/251‑1 (MQ=255)                                                                            tCCAGAAAGTTTTCAGTTCAAAGTGCGGTGGATTTTTTAATTGAAAATGATACAGACTGCAACGACGGGCGGAGTCGATTTTGTGAATATCGCCGTAATCGGCAAGCATTTTTTCGGCGGAACGCACACCACTACGATTTTCGCCCACCAGTAAAACTTCCTGTCCGGCGGTACATTGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGgcg                                                                                                                                                                       >  1:55890/1‑251 (MQ=255)                                                                                       ttCAGTTCAAAGTGCGGTGGATTTTTTAATTGAAAATGATACAGACTGCAACGACGGGCGGAGTCGATTTTGTGAATATCGCCGTAATCGGCAAGCATTTTTTCGGCGGAACGCACACCACTACGATTTTCGCCCACCAGTAAAACTTCCTGTCCGGCGGTACATTGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGaaa                                                                                                                                                            <  1:821885/251‑1 (MQ=255)                                                                                                                      gAAAATGATACAGACTGCAACGACGGGCGGAGTCGATTTTGTGAATATCGCCGTAATCGGCAAGCATTTTTTCGGCGGAACGCACACCACTACGATTTTCGCCCACCAGTAAAACTTCCTGTCCGGCGGTACATTGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTgg                                                                                                                              >  1:53459/1‑250 (MQ=255)                                                                                                                        aaaTGATACAGACTGCAACGACGGGCGGAGTCGATTTTGTGAATATCGCCGTAATCGGCAAGCATTTTTTCGGCGGAACGCACACCACTACGATTTTCGCCCACCAGTAAAACTTCCTGTCCGGCGGTACATTGTGAGAGAAGCTGCATGAGCTGGaaa                                                                                                                                                                                                                       >  1:772392/1‑159 (MQ=255)                                                                                                                          aTGATACAGACTGCAACGACGGGCGGAGTCGATTTTGTGAATATCGCCGTAATCGGCAAGCATTTTTTCGGCGGAACGCACACCACTACGATTTTCGCCCACCAGTAAAACTTCCTGTCCGGCGGTACAt                                                                                                                                                                                                                                                  >  1:392336/1‑130 (MQ=255)                                                                                                                          aTGATACAGACTGCAACGACGGGCGGAGTCGATTTTGTGAATATCGCCGTAATCGGCAAGCATTTTTTCGGCGGAACGCACACCACTACGATTTTCGCCCACCAGTAAAACTTCCTGTCCGGCGGTACATTGTgagag                                                                                                                                                                                                                                          >  1:105046/1‑138 (MQ=255)                                                                                                                          aTGATACAGACTGCAACGACGGGCGGAGTCGATTTTGTGAATATCGCCGTAATCGGCAAGCATTTTTTCGGCGGAACGCACACCACTACGATTTTCGCCCACCAGTAAAACTTCCTGTCCGGCGGTACATTGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAggg                                                                                                                                                    >  1:105341/1‑224 (MQ=255)                                                                                                                          aTGATACAGACTGCAACGACGGGCGGAGTCGATTTTGTGAATATCGCCGTAATCGGCAAGCATTTTTTCGGCGGAACGCACACCACTACGATTTTCGCCCACCAGTAAAACTTCCTGTCCGGCGGTACATTGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAAt                                                                                                                                              <  1:520717/230‑1 (MQ=255)                                                                                                                            gATACAGACTGCAACGACGGGCGGAGTCGATTTTGTGAATATCGCCGTAATCGGCAAGCATTTTTTCGGCGGAACGCACACCACTACGATTTTCGCCCACCAGTAAAACTTCCTGTCCGGCGGTACATTGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTCCTGTTTAttc                                                                                                                                                                                                     >  1:311964/1‑173 (MQ=255)                                                                                                                             aTACAGACTGCAACGACGGGCGGAGTCGATTTTGTGAATATCGCCGTAATCGGCAAGCATTTTTTCGGCGGAACGCACACCACTACGATTTTCGCCCACCAGTAAAACTTCCTGTCCGGCGGTACATTGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTggt                                                                                                                      >  1:414259/1‑249 (MQ=255)                                                                                                                                    cTGCAACGACGGGCGGAGTCGATTTTGTGAATATCGCCGTAATCGGCAAGCATTTTTTCGGCGGAACGCACACCACTACGATTTTCGCCCACCAGTAAAACTTCCTGTCCGGCGGTACATTGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATcaca                                                                                                                                                                             <  1:807440/189‑1 (MQ=255)                                                                                                                                    cTGCAACGACGGGCGGAGTCGATTTTGTGAATATCGCCGTAATCGGCAAGCATTTTTTCGGCGGAACGCACACCACTACGATTTTCGCCCACCAGTAAAACTTCCTGTCCGGCGGTACATTGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACttt                                                                                                                                  >  1:233494/1‑232 (MQ=255)                                                                                                                                    cTGCAACGACGGGCGGAGTCGATTTTGTGAATATCGCCGTAATCGGCAAGCATTTTTTCGGCGGAACGCACACCACTACGATTTTCGCCCACCAGTAAAACTTCCTGTCCGGCGGTACATTGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTggtg                                                                                                                     <  1:741319/245‑4 (MQ=255)                                                                                                                                      gCAACGACGGGCGGAGTCGATTTTGTGAATATCGCCGTAATCGGCAAGCATTTTTTCGGCGGAACGCACACCACTACGATTTTCGCCCACCAGTAAAACTTCCTGTCCGGCGGTACATTGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTcggt                                                                                                             >  1:369840/1‑248 (MQ=255)                                                                                                                                                 cggAGTCGATTTTGTGAATATCGCCGTAATCGGCAAGCATTTTTTCGGCGGAACGCACACCACTACGATTTTCGCCCACCAGTAAAACTTCCTGTCCGGCGGTACATTGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTc                                                                                                   <  1:381376/250‑1 (MQ=255)                                                                                                                                                       cGATTTTGTGAATATCGCCGTAATCGGCAAGCATTTTTTCGGCGGAACGCACACCACTACGATTTTCGCCCACCAGTAAAACTTCCTGTCCGGCGGTACATTGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATa                                                                                             >  1:871367/1‑250 (MQ=255)                                                                                                                                                       cGATTTTGTGAATATCGCCGTAATCGGCAAGCATTTTTTCGGCGGAACGCACACCACTACGATTTTCGCCCACCAGTAAAACTTCCTGTCCGGCGGTACATTGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATAc                                                                                            <  1:786551/251‑1 (MQ=255)                                                                                                                                                           tttGTGAATATCGCCGTAATCGGCAAGCATTTTTTCGGCGGAACGCACACCACTACGATTTTCGCCCACCAGTAAAACTTCCTGTCCGGCGGTACATTGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGgcgc                                                                                                                                                                      <  1:539352/173‑1 (MQ=255)                                                                                                                                                                   tatCGCCGTAATCGGCAAGCATTTTTTCGGCGGAACGCACACCACTACGATTTTCGCCCACCAGTAAAACTTCCTGTCCGGCGGTACATTGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATgcg                                                                                <  1:220115/251‑1 (MQ=255)                                                                                                                                                                       gCCGTAATCGGCAAGCATTTTTTCGGCGGAACGCACACCACTACGATTTTCGCCCACCAGTAAAACTTCCTGTCCGGCGGTACATTGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGgcgc                                                                                                                                                                      >  1:804373/1‑161 (MQ=255)                                                                                                                                                                        ccGTAATCGGCAAGCATTTTTTCGGCGGAACGCACACCACTACGATTTTCGCCCACCAGTAAAACTTCCTGTCCGGCGGTACATTGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGgcgc                                                                                                                                                                      <  1:294721/160‑1 (MQ=255)                                                                                                                                                                          gTAATCGGCAAGCATTTTTTCGGCGGAACGCACACCACTACGATTTTCGCCCACCAGTAAAACTTCCTGTCCGGCGGTACATTGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGt                                                                         >  1:272452/1‑251 (MQ=255)                                                                                                                                                                                      cATTTTTTCGGCGGAACGCACACCACTACGATTTTCGCCCACCAGTAAAACTTCCTGTCCGGCGGTACATTGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTggc                                                             <  1:516195/251‑3 (MQ=255)                                                                                                                                                                                                   gAACGCACACCACTACGATTTTCGCCCACCAGTAAAACTTCCTGTCCGGCGGTACATTGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTc                                                                                      <  1:688054/213‑1 (MQ=255)                                                                                                                                                                                                            ccacTACGATTTTCGCCCACCAGTAAAACTTCCTGTCCGGCGGTACATTGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAat                                        >  1:800593/4‑250 (MQ=255)                                                                                                                                                                                                               cTACGATTTTCGCCCACCAGTAAAACTTCCTGTCCGGCGGTACATTGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTc                                                                                                   <  1:506759/188‑1 (MQ=255)                                                                                                                                                                                                                      tttCGCCCACCAGTAAAACTTCCTGTCCGGCGGTACATTGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTcacc                             <  1:86709/251‑4 (MQ=255)                                                                                                                                                                                                                               ccaGTAAAACTTCCTGTCCGGCGGTACATTGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCAcccc                        >  1:748261/1‑247 (MQ=255)                                                                                                                                                                                                                                cagtaaaaCTTCCTGTCCGGCGGTACATTGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGa                   >  1:70827/5‑251 (MQ=255)                                                                                                                                                                                                                                  gtaaaaCTTCCTGTCCGGCGGTACATTGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGa                   >  1:69385/3‑249 (MQ=255)                                                                                                                                                                                                                                                   gcggTACATTGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCg  >  1:621028/1‑249 (MQ=255)                                                                                                                                                                                                                                                   gcggTACATTGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCg  >  1:374102/1‑249 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                    CCAGTTCGGCGGCGCTGAATACGCCGGGCAACGCATAGATAGTTAAATCGGCAATTTGTGGCGATTGATAGGATTTCCAGAAAGTTTTCAGTTCAAAGTGCGGTGGATTTTTTAACTGAAAATGATACAGACTGCAACGGCGGGCGGAGTCGATTTTGTGAATATCGCCGTAATCGGCAAGCATTTTTTCGGCGGAACGCACGCCGCTACGATTTTCGCCCACCAGCAAAACCTCCTGCCCGGCGGTGCATTGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGATGTTGTCTGTTGGCATAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGACGTTGTAAATCGGCGAGAAAATCATCGTTCGCCGCCCCGCTGAGCAGCAGGGACTTCCCG  >  1/1828836‑1829326

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑