Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,828,787	C→T	intergenic (+46887/+6597)	unknown → / ← unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,828,787	0	C	T	100.0%	192.2 / NA	65	intergenic (+46887/+6597)	unknown/unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (39/26);  total (39/26)

GTGTCAATGCCATCATGAAACGGCGGGTTGGAAATGATCAGATCAAATTTGCCGTCAATATGGGAAAACACATCACTGGCAACCACTGTTCCCTGTAGCCGATTTTCCTGTAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCATGAATATCTGTCATGATTAATCGGGTGTTCGGGTTGTGCCGTTGAATATAACTGCCGATCACTCCCGCGCCGCAGCCCACGTCCAACACCCCGCCTGCGATGTTTTTTAGCGTAGAAAGCAATAATGCCGTGCCGCCATCCAGTTCGGCGGCGCTGAATACGCCGGGCAACGCATAGATAGTTAAATCGGCAATTTGTGGCGATTGATAGGATTTCCAGAAAGTTTTCAGTTCAAAGTGCGGTGGATTTTTTAACTGAAAATGATACAGACTGCAACGGCGGGCGGAGTCGATTTTGTGAATATCGCCGTAATCGGCAAGCATTTTTTCGGCGGAACGCAC  >  1/1828553‑1829037                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                           gtgtCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATATGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTGTAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGttttt                                                                                                                                                                                                                                            >  1:274544/1‑251 (MQ=255)            gtcatGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATATGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTGTAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGaaa                                                                                                                                                                                                                                 >  1:108366/2‑251 (MQ=255)            gtcatGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATATGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTGTAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGaaa                                                                                                                                                                                                                                 >  1:534545/2‑251 (MQ=255)              catGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATATGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTGTAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGc                                                                                                                                                                                                                               <  1:764399/251‑1 (MQ=255)                  aaaaggcggATTGGAAATGATCAGATCGAATTTGCCGTCAATATGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTGTAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGCaat                                                                                                                                                                                                                            <  1:278914/246‑1 (MQ=255)                       gcggATTGGAAATGATCAGATCGAATTTGCCGTCAATATGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTGTAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGCAATAAtgc                                                                                                                                                                                                                       <  1:142918/250‑1 (MQ=255)                          gattGGAAATGATCAGATCGAATTTGCCGTCAATATGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTGTAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGCAATAAtgccgtg                                                                                                                                                                                                                   >  1:93684/3‑251 (MQ=255)                              ggAAATGATCAGATCGAATTTGCCGTCAATATGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTGTAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGtttt                                                                                                                                                                                                                                             >  1:436831/1‑221 (MQ=255)                               gAAATGATCAGATCGAATTTGCCGTCAATATGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTGTAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGCAATAATGCCGTgccgc                                                                                                                                                                                                               >  1:619016/1‑250 (MQ=255)                                   tGATCAGATCGAATTTGCCGTCAATATGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTGTAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGCAATAATGCCGTGCCGCCATcc                                                                                                                                                                                                          <  1:706324/251‑1 (MQ=255)                                   tGATCAGATCGAATTTGCCGTCAATATGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTGTAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGCAATAATGCCGTGCCGCCATcc                                                                                                                                                                                                          <  1:893288/251‑1 (MQ=255)                                              aaTTTGCCGTCAATATGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTGTAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGCAATAATGCCGTGCCGCCATCCAGCTcggcgg                                                                                                                                                                                                <  1:38442/250‑1 (MQ=255)                                                       tCAATATGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTGTAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGCAATAATGCCGTGCCGCCATCCAGCTCGGCGGCGCTGAATAc                                                                                                                                                                                      <  1:663360/251‑1 (MQ=255)                                                        cAATATGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTGTAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGCAATAATGCCGTGCCGCCATCCAGCTCGGCGGCGCTGAATACg                                                                                                                                                                                     >  1:843908/1‑251 (MQ=255)                                                               ggAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTGTAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGCAATAATGCCGTGCCGCCATCCAGCTCGGCGGCGCTGAATAc                                                                                                                                                                                      >  1:360564/1‑243 (MQ=255)                                                                         tCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTGTAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGCAATAATGCCGTGCCGCCATCCAGCTCGGCGGCGCTGAATACGCCGGGCAACGCatagat                                                                                                                                                                    <  1:344180/251‑1 (MQ=255)                                                                              ggCAACCACCGTGCCTTGCAACCGATTTTCCTGTAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGtttttt                                                                                                                                                                                                                                           <  1:865054/175‑1 (MQ=255)                                                                              ggCAACCACCGTGCCTTGCAACCGATTTTCCTGTAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGCAATAATGCCGTGCCGCCATCCAGCTCGGCGGCGCTGAATACGCCGGGCAACGCatagat                                                                                                                                                                    <  1:485938/246‑1 (MQ=255)                                                                                caaccaccgtgccttgcaaccGATTTTCCTGTAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGCAATAATGCCGTGCCGCCATCCAGCTCGGCGGCGCTGAATACGCCGGGCAACGCATAGATAGTTaa                                                                                                                                                              <  1:125533/231‑1 (MQ=255)                                                                                  accaccgtgccttgcaaccGATTTTCCTGTAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGCAATAATGCCGTGCCGCCATCCAGCTCGGCGGCGCTGAATACGCCGGGCAACGCatagat                                                                                                                                                                    >  1:197010/18‑242 (MQ=255)                                                                                   ccaccgtgccttgcaaccGATTTTCCTGTAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGCAATAATGCCGTGCCGCCATCCAGCTCGGCGGCGCTGAATACGCCGGGCAACGCATAGATAGTTAAATCg                                                                                                                                                          >  1:741085/17‑251 (MQ=255)                                                                                        gtgccttgcaaccGATTTTCCTGTAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGCAATAATGCCGTGCCGCCATCCAGCTCGGCGGCGCTGAATACGCCGGGCAACGc                                                                                                                                                                          >  1:489031/12‑230 (MQ=255)                                                                                          gccttgcaaccGATTTTCCTGTAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGCAATAATGCCGTGCCGCCATCCAGCTCGGCGGCGCTGAATACGCCGGGCAACGCATAGATAGTTAAATCGGCaagtt                                                                                                                                                   >  1:583796/10‑248 (MQ=255)                                                                                               gcaaccGATTTTCCTGTAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAg                                                                                                                                                                                                                                         >  1:304257/5‑160 (MQ=255)                                                                                                     gATTTTCCTGTAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGCAATAATGCCGTGCCGCCATCCAGCTCGGCGGCGCTGAATACGCCGGGCAACGCATAGATAGTTAAATCGGCAAGTTGCGACGATTGa                                                                                                                                        >  1:392892/1‑251 (MQ=255)                                                                                                      aTTTTCCTGTAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGCAATAATGCCGTGCCGCCATCCAGCTCGGCGGCGCTGAATACGCCGGGCAACGCATAGATAGTTAAATCGGCAAGTTGCGACGATTGAt                                                                                                                                       <  1:646635/251‑1 (MQ=255)                                                                                                          tCCTGTAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGCAATAATGCCGTGCCGCCATCCAGCTCGGCGGCGCTGAATACGCCGGGCAACGCATAGATAGTTAAATCGGCAAGTTGCGACGATTGATAGGa                                                                                                                                   <  1:555053/251‑1 (MQ=255)                                                                                                           ccTGTAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGCAATAATGCCGTGCCGCCATCCAGCTCGGCGGCGCTGAATACGCCGGGCAACGCATAGATAGTTAAATCGGCAAGTTGCGACGATTGATAGGAt                                                                                                                                  >  1:544524/1‑251 (MQ=255)                                                                                                                aaaGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGCAATAATGCCGTGCCGCCATCCAGCTCGGCGGCGCTGAATACGCCGGGCAACGCatagat                                                                                                                                                                    >  1:50987/1‑212 (MQ=255)                                                                                                                aaaGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGCAATAATGCCGTGCCGCCATCCAGCTCGGCGGCGCTGAATACGCCGGGCAACGCATAGATAGTTAAATCGGCAAGTTGCGACGATTGATAGGATTTCCa                                                                                                                             >  1:252635/1‑251 (MQ=255)                                                                                                                          ttGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGCAATAAtgc                                                                                                                                                                                                                       <  1:369104/151‑1 (MQ=255)                                                                                                                          ttGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGCAATAATGCCGTGCCGCCATCCAGCTCGGCGGCGCTGAATACGCCGGGCAACGCATAGATAGTTAAATCGGCAAGTTGCGACGATTGATAGGATTTCCAGAAAGtttt                                                                                                                    >  1:857387/1‑250 (MQ=255)                                                                                                                                 aGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGCAATAATGCCGTGCCGCCATCCAGCTCGGCGGCGCTGAATACGCCGGGCAACGCatagat                                                                                                                                                                    >  1:778693/1‑195 (MQ=255)                                                                                                                                             ggcgtGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGCAATAATGCCGTGCCGCCATCCAGCTCGGCGGCGCTGAATACGCCGGGCAACGCATAGATAGTTaa                                                                                                                                                              <  1:547022/185‑1 (MQ=255)                                                                                                                                              gcgtGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGCAATAAtgc                                                                                                                                                                                                                       <  1:800835/128‑1 (MQ=255)                                                                                                                                                gtGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGCAATAATGCCGTGCCGCCATCCAGCTCGGCGGCGCTGAATACGCCGGGCAACGCATAGATAGTTAAATCGGCAAGTTGCGACGATTGATAGGATTTCCAGAAAGTTTTCAGTTCAAAGTGCGGTGGAtttt                                                                                             >  1:174457/2‑251 (MQ=255)                                                                                                                                                   aaTATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGCAATAATGCCGTGCCGCCATCCAGCTCGGCGGCGCTGAATACGCCGGGCAACGCATAGATAGTTAAATCGGCAAGTTGCGACGATTGATAGGATTTCCAGAAAGTTTTCAGTTCAAAGTGCGGTGGATTTTTTa                                                                                          >  1:152147/1‑251 (MQ=255)                                                                                                                                                            cATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGCAATAATGCCGTGCCGCCATCCAGCTCGGCGGCGCTGAATACGCCGGGCAACGCATAGATAGTTAAATCGGCAAGTTGCGACGATTGATAGGATTTCCAGAAAGTTTTCAGTTCAAAGTGCGGTGGATTTTTTAATTGAAAAt                                                                                 <  1:618059/251‑1 (MQ=255)                                                                                                                                                               gATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGCAATAATGCCGTGCCGCCATCCAGCTCGGCGGCGCTGAAt                                                                                                                                                                                        <  1:855753/145‑1 (MQ=255)                                                                                                                                                                aTTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGCAATAAtgc                                                                                                                                                                                                                       <  1:344206/113‑1 (MQ=255)                                                                                                                                                                   aaTCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGCAATAATGCCGTGCCGCCATCCAGCTCGGCGGCGCTGAATACGCCGGGCAACGCATAGATAGTTAAATCGGCAAGTTGCGACGATTGATAGGATTTCCAGAAAGTTTTCAGTTCAAAGTGCGGTGGATTTTTTAATTGAAAATGATACAg                                                                          <  1:730085/251‑1 (MQ=255)                                                                                                                                                                           gtTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGCAATAATGCCGTGCCGCCATCCAGCTCGGCGGCGCTGAATACGCCGGGCAACGCATAGATAGTTAAATCGGCAAGTTGCGACGATTGATAGGATTTCCAGAAAGTTTTCAGTTCAAAGTGCGGTGGATTTTTTAATTGAAAATGATACAGACTGCaa                                                                   >  1:850159/1‑250 (MQ=255)                                                                                                                                                                           gtTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGCAATAATGCCGTGCCGCCATCCAGCTCGGCGGCGCTGAATACGCCGGGCAACGCATAGATAGTTAAATCGGCAAGTTGCGACGATTGATAGGATTTCCAGAAAGTTTTCAGTTCAAAGTGCGGTGGATTTTTTAATTGAAAATGATACAGACTGCaa                                                                   >  1:732606/1‑250 (MQ=255)                                                                                                                                                                            ttCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGCAATAATGCCGTGCCGCCATCCAGCTCGGCGGCGCTGAATACGCCGGGCAACGCATAGATAGTTAAATCGGCAAGTTGCGACGATTGATAGGATTTCCAGAAAGTTTTCAGTTCAAAGTGCGGTGGATTTTTTAATTGAAAATGATACAGACTGCAAc                                                                  <  1:257430/250‑1 (MQ=255)                                                                                                                                                                             tCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGCAATAATGCCGTGCCGCCATCCAGCTCGGCGGCGCTGAATACGCCGGGCAACGCATAGATAGTTAAATCGGCAAGTTGCGACGATTGATAGGATTTCCAGAAAGTTTTCAGTTCAAAGTGCGGTGGATTTTTTAATTGAAAATGATACAGACTGCAAcga                                                                <  1:792504/251‑2 (MQ=255)                                                                                                                                                                                ggTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGTTGTTTTTTAGCGTAGAAAGCAATAATGCCGTGCCGCCATCCAGCTCGGCGGCGCTGAATACGCCGGGCAACGCATAGATAGTTAAATCGGCAAGTTGCGACGATTGATAGGATTTCCAGAAAGTTTTCAGTTCAAAGTGCGGTGGATTTTTTAATTGAAAATGATAc                                                                            >  1:304567/1‑236 (MQ=255)                                                                                                                                                                                         gTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGCAATAATGCCGTGCCGCCATCCAGCTCGGCGGCGCTGAATACGCCGGGCAACGCATAGATAGTTAAATCGGCAAGTTGCGACGATTGATAGGATTTCCAGAAAGTTTTCAGTTCAAAGTGCGGTGGATTTTTTAATTGAAAATGATACAGACTGCAAcgacg                                                              >  1:129192/1‑238 (MQ=255)                                                                                                                                                                                         gTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGCAATAATGCCGTGCCGCCATCCAGCTCGGCGGCGCTGAATACGCCGGGCAACGCATAGATAGTTAAATCGGCAAGTTGCGACGATTGATAGGATTTCCAGAAAGTTTTCAGTTCAAAGTGCGGTGGATTTTTTAATTGAAAATGATACAGACTGCAACGACGGGCGGAGTCg                                                    >  1:151337/1‑251 (MQ=255)                                                                                                                                                                                         gTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGCAATAATGCCGTGCCGCCATCCAGCTCGGCGGCGCTGAATACGCCGGGCAACGCATAGATAGTTAAATCGGCAAGTTGCGACGATTGATAGGATTTCCAGAAAGTTTTCAGTTCAAAGAGCGGTGGATTTTTTAATTGAAAATGATACAGACTGCAACGACGGGCGGAGTCg                                                    >  1:510831/1‑251 (MQ=255)                                                                                                                                                                                            gAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGCAATAATGCCGTGCCGCCATCCAGCTCGGCGGCGCTGAATACGCCGGGCAACGCATAGATAGTTAAATCGGCAAGTTGCGACGATTGATAGGATTTCCAGAAAGTTTTCAGTTCAAAGTGCGGTGGATTTTTTAATTGAAAATGATACAGACTGCAACGACGGGCGGAGTCGAtt                                                 >  1:180815/1‑251 (MQ=255)                                                                                                                                                                                            gAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGCAATAATGCCGTGCCGCCATCCAGCTCGGCGGCGCTGAATACGCCGGGCAACGCATAGATAGTTAAATCGGCAAGTTGCGACGATTGATAGGATTTCCAGAAAGTTTTCAGTTCAAAGTGCGGTGGATTTTTTAATTGAAAATGATACAGACTGCAACGACGGGCGGAGTCGAtt                                                 >  1:658031/1‑251 (MQ=255)                                                                                                                                                                                              atatAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGCAATAATGCCGTGCCGCCATCCAGCTCGGCGGCGCTGAATACGCCGGGCAACGCATAGATAGTTAAATCGGCAAGTTGCGACGATTGATAGGATTTCCAGAAAGTTTTCGGTTCAAAGTGCGGTGGATTTTTTAATTGAAAATGATACAGACTGCAACGACGGGCGGAGTCGAtttt                                               >  1:615956/1‑251 (MQ=255)                                                                                                                                                                                                      gccaaTCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGCAATAATGCCGTGCCGCCATCCAGCTCGGCGGCGCTGAATACGCCGGGCAACGCATAGATAGTTAAATCGGCAAGTTGCGACGATTGATAGGATTTCCAGAAAGTTTTCAGTTCAAAGTGCGGTGGATTTTTTAATTGAAAATGATACAGACTGCAACGACGGGCGGAGTCGATTTTGTGAatat                                       >  1:791005/5‑251 (MQ=255)                                                                                                                                                                                                       ccGATCACTCCCGCGCCGCAGCCCACGTCCAGCACCTCGCCTGCGATGTTTTTTAGCGTAGAAAGCAATAATGCCGTGCCGCCATCCAGTTCGGCGGCGCTGAATACGCCGGGCAACGCATAGATAGTTAAATCGGCAATTTGTGGCGATTGATAGGATTTCCAGAAAGTTTTCAGTTCAAAGTGCGGTGGATTTTTTAACTGAAAATGATACAGACTGCAACGGCGGGCGGAGTCGATTTTGTGAatat                                       <  1:11417/250‑1 (MQ=255)                                                                                                                                                                                                         aaTCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGCAATAATGCCGTGCCGCCATCCAGCTCGGCGGCGCTGAATACGCCGGGCAACGCATAGATAGTTAAATCGGCAAGTTGCGACGATTGATAGGATTTCCAGAAAGTTTTCAGTTCAAAGTGCGGTGGATTTTTTAATTGAAAATGATACAGACTGCAACGACGGGCGGAGTCGATTTTGTGAATATCg                                     >  1:393609/2‑250 (MQ=255)                                                                                                                                                                                                            cacccctgcgcCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGCAATAATGCCGTGCCGCCATCCAGCTCGGCGGCGCTGAATACGCCGGGCAACGCATAGATAGTTAAATCGGCAAGTTGCGACGATTGATAGGATTTCCAGAAAGTTTTCAGTTCAAAGTGCGGTGGATTTTTTAATTGAAAATGATACAGACTGCAACGACGGGCGGAGTCGATTTTGTGAATATCGCCGt                                 >  1:839009/8‑251 (MQ=255)                                                                                                                                                                                                                        gcAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGCAATAATGCCGTGCCGCCATCCAGCTCGGCGGCGCTGAATACGCCGGGCAACGCATAGATAGTTAAATCGGCAAGTTGCGACGATTGATAGGATTTCCAGAAAGTTTTCAGTTCAAAGTGCGGTGGATTTTTTAATTGAAAATGATACAGACTGCAACGACGGGCGGAGTCGATTTTGTGAATATCGCCGTAATCGGCAAGCa                     >  1:374079/1‑251 (MQ=255)                                                                                                                                                                                                                           gCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGCAATAATGCCGTGCCGCCATCCAGCTCGGCGGCGCTGAATACGCCGGGCAACGCATAGATAGTTAAATCGGCAAGTTGCGACGATTGATAGGATTTCCAGAAAGTTTTCAGTTCAAAGTGCGGTGGATTTTTTAATTGAAAATGATACAGACTGCAACGACGGGCGGAGTCGATTTTGTGAatat                                       >  1:381684/1‑230 (MQ=255)                                                                                                                                                                                                                                 gTCCAACGCCTCGCCCGCGATGTTTTTTAGCGTAGAAAGCAATAATGCCGTGCCGCCATCCAGCTCGGCGGCGCTGAATACGCCGGGCAACGCATAGATAGTTAAATCGGCAAGTTGCGACGATTGATAGGATTTCCAGAAAGTTTTCAGTTCAAAGTGCGGTGGATTTTTTAATTGAAAATGATACAGACTGCAACGACGGGCGGAGTCGATTTTGTGAATATCGCCGTAATCGGCAAGCAtttttt               <  1:751717/248‑1 (MQ=255)                                                                                                                                                                                                                                 gTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGCAATAATGCCGTGCCGCCATCCAGCTCGGCGGCGCTGAATACGCCGGGCAACGCATAGATAGTTAAATCGGCAAGTTGCGACGATTGATAGGATTTCCAGAAAGTTTTCAGTTCAAAGTGCGGTGGATTTTTTAATTGAAAATGATACAGACTGCAACGACGGGCGGAGTCGATTTTGTGa                                           <  1:726661/220‑1 (MQ=255)                                                                                                                                                                                                                                 gTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGCAATAATGCCGTGCCGCCATCCAGCTCGGCGGCGCTGAATACGCCGGGCAACGCATAGATAGTTAAATCGGCAAGTTGCGACGATTGATAGGATTTCCAGAAAGTTTTCAGTTCAAAGTGCGGTGGATTTTTTAATTGAAAATGATACAGACTGCAACGACGGGCGGAGTCGATTTTGTGAata                                        <  1:716407/223‑1 (MQ=255)                                                                                                                                                                                                                                 gTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGCAATAATGCCGTGCCGCCATCCAGCTCGGCGGCGCTGAATACGCCGGGCAACGCATAGATAGTTAAATCGGCAAGTTGCGACGATTGATAGGATTTCCAGAAAGTTTTCAGTTCAAAGTGCGGTGGATTTTTTAATTGAAAATGATACAGACTGCAACGACGGGCGGAGTCGATTTTGTGAATATCGCCGTAATCGGCAAGCATTTTTTcgg            >  1:406534/1‑251 (MQ=255)                                                                                                                                                                                                                                 gTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGCAATAATGCCGTGCCGCCATCCAGCTCGGCGGCGCTGAATACGCCGGGCAACGCATAGATAGTTAAATCGGCAAGTTGCGACGATTGATAGGATTTCCAGAAAGTTTTCAGTTCAAAGTGCGGTGGATTTTTTAATTGAAAATGATACAGACTGCAACGACGGGCGGAGTCGATTTTGTGAATATCGCCGTAATCGGCAAGCATTTTTTcgg            >  1:364997/1‑251 (MQ=255)                                                                                                                                                                                                                                 gTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGCAATAATGCCGTGCCGCCATCCAGCTCGGCGGCGCTGAATACGCCGGGCAACGCATAGATAGTTAAATCGGCAAGTTGCGACGATTGATAGGATTTCCAGAAAGTTTTCAGTTCAAAGTGCGGTGGATTTTTTAATTGAAAATGATACAGACTGCAACGACGGGCGGAGTCGATTTTGTGAATATCGCCGTAATCGGCAAGCATTTTTTcgg            >  1:874023/1‑251 (MQ=255)                                                                                                                                                                                                                                       cacCTCGCCCGCGATGTTTTTTAGCGTAGAAAGCAATAATGCCGTGCCGCCATCCAGCTCGGCGGCGCTGAATACGCCGGGCAACGCATAGATAGTTAAATCGGCAAGTTGCGACGATTGATAGGATTTCCAGAAAGTTTTCAGTTCAAAGTGCGGTGGATTTTTTAATTGAAAATGATACAGACTGCAACGACGGGCGGAGTCGATTTTGTGAATATCGCCGTAATCGGCAAGCATTTTTTCGTCGGaa       >  1:276473/1‑250 (MQ=255)                                                                                                                                                                                                                                           ccgcccgcGATGTTTTTTAGCGTAGAAAGCAATAATGCCGTGCCGCCATCCAGCTCGGCGGCGCTGAATACGCCGGGCAACGCATAGATAGTTAAATCGGCAAGTTGCGACGATTGATAGGATTTCCAGAAAGTTTTCAGTTCAAAGTGCGGTGGATTTTTTAATTGAAAATGATACAGACTGCAACGACGGGCGGAGTCGATTTTGTGAATATCGCCGTAATCGGCAAGCATTTTTTCGGCGGAacgcac  <  1:782685/251‑1 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                           GTGTCAATGCCATCATGAAACGGCGGGTTGGAAATGATCAGATCAAATTTGCCGTCAATATGGGAAAACACATCACTGGCAACCACTGTTCCCTGTAGCCGATTTTCCTGTAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCATGAATATCTGTCATGATTAATCGGGTGTTCGGGTTGTGCCGTTGAATATAACTGCCGATCACTCCCGCGCCGCAGCCCACGTCCAACACCCCGCCTGCGATGTTTTTTAGCGTAGAAAGCAATAATGCCGTGCCGCCATCCAGTTCGGCGGCGCTGAATACGCCGGGCAACGCATAGATAGTTAAATCGGCAATTTGTGGCGATTGATAGGATTTCCAGAAAGTTTTCAGTTCAAAGTGCGGTGGATTTTTTAACTGAAAATGATACAGACTGCAACGGCGGGCGGAGTCGATTTTGTGAATATCGCCGTAATCGGCAAGCATTTTTTCGGCGGAACGCAC  >  1/1828553‑1829037

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑