Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,828,696	A→G	intergenic (+46796/+6688)	unknown → / ← unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,828,696	0	A	G	100.0%	200.7 / NA	73	intergenic (+46796/+6688)	unknown/unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (39/34);  total (39/34)

AAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCATCATGAAACGGCGGGTTGGAAATGATCAGATCAAATTTGCCGTCAATATGGGAAAACACATCACTGGCAACCACTGTTCCCTGTAGCCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCATGAATATCTGTCATGATTAATCGGGTGTTCGGGTTGTGCCGTTGAATATAACTGCCGATCACTCCCGCGCCGCAGCCCACGTCCAACACCCCGCCTGCGATGTTTTTTAGCGTAGAAAGCAATAATGCCGTGCCGCCATCCAGTTCGGCGGCGCTGAATACGCCGGGCAACGCATAGATAGTTAAATCGGCAATTTGTGGCGATTGATAGGATTTCCAGAAAGTTTT  >  1/1828458‑1828923                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                    aaGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATATGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTc                                                                                                                                                                                                                         <  1:626801/251‑1 (MQ=255)       gAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATATGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAat                                                                                                                                                                                                                                <  1:601032/238‑1 (MQ=255)        aaaCGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATATGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGa                                                                                                                                                                                                                     <  1:806713/248‑1 (MQ=255)        aaaCGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATATGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGAtt                                                                                                                                                                                                                   <  1:762075/250‑1 (MQ=255)                  gCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGGGTCAATGCCGTCATGAAAAGGCGGGTTGGGAATGATCAGATCGAATTTGCCGTCAATATGGGGAAACAAGTCACTGGCAACCACCCTGCCTTGCAACCGATTTTTCTG‑TAAAATGCGCTTTGCGGAAGCCAACGCCAAGGCGGGAATATCCGTCATGATTAATCGGGGGtt                                                                                                                                                                                                        >  1:597229/1‑251 (MQ=255)                      cGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATATGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCggg                                                                                                                                                                                                    <  1:25843/251‑1 (MQ=255)                        aTGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATATGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGt                                                                                                                                                                                                   <  1:2235/250‑1 (MQ=255)                        aTGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGGAAGCGGGGTCAAAGGCGTCATGAAAAGGGGGGATGGGAAAGATTAAAACGAATTTGGCGTTAATATGGGGAAACACGGTACCGGGAACCACCCGGCCCTGCAGCCGATTTTTCTGTTAAAG‑GCGCCTTGCGGGAGCCAACGCCAAGGCGGGGAAATTCGTCATGATTAATCggggggttggggt                                                                                                                                                                                                  >  1:456001/1‑241 (MQ=255)                          gcgcAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAAGCGGGTTGGAAATGATCAGATCGAATTTGCCGTCAATATGGGAAAAAACGTCACTGGGAACCACCGTGCCTTGCAACCGCTTTTCCTG‑TAAAGTGCGCTTTGCGGGAGCCAACGCCAAGGCGTGGATATCCGTCCTGATTAATctgggggtcgggg                                                                                                                                                                                                   >  1:652145/1‑236 (MQ=255)                          gcgcAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGGGTCAATGCCGTCATGAAAAGGCGGATTGGGAATGATCAGATTGAATTTTCCGGCAATATGGGGAAAAACGGCAATGGCAACCACCCTGCCTTGCAACCGATTTTTCTG‑TAAAGTGCGCTTTGCGGGAGCCAACGCCCAGGGGGGAATATTCCTCATGATTAATCGGGGGTTCggggtgg                                                                                                                                                                                                >  1:56099/1‑247 (MQ=255)                             cAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATATGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGc                                                                                                                                                                                              <  1:116878/250‑1 (MQ=255)                             cAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATATGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGc                                                                                                                                                                                              <  1:725967/250‑1 (MQ=255)                               gCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAACGGGGGCAATGCCGTCCTTAAAAAGCGGGTTGGGAAAGATCAGATCGAATTTGCCGGCCATATGGGGAAAAACGTCACTGGCCACCCCCGGGCCTTTCAACCGATTTTTCTG‑TAAAGAGCGCTTTGCGGGAGCCCACGCCAAGGCGTGAATATCCGTCCTGATTAATCGGGGGTTCGGGGTGTGctgg                                                                                                                                                                                           >  1:889829/1‑248 (MQ=255)                                       cccGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATATGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAatat                                                                                                                                                                                    <  1:294368/250‑1 (MQ=255)                                         cGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATATGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAAc                                                                                                                                                                                 <  1:763838/251‑1 (MQ=255)                                             ttCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGGGTCAATGCCGTCCTGAAAAGGCGGGTTGGGAATGATCAGATCGAATTTTCCGTCAATATGGGGAAAAACGTCACTGGCAACCACCCTGCCTTGCAAACGATTTTCCTG‑TAAAATGCGCTTTTCGGAAGACAACGCCAAGGCGGGAATATCCGTCATGATTAAACGGGGGTTCGGGGTGTGCTGTTGAATATAAATGc                                                                                                                                                                              >  1:830181/1‑250 (MQ=255)                                               cAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGGGTCAAAGCCGTCATGAAAAGGGGGATTGGGAATGAAAAGATCGAATTTGCCGTCAATATGGGAAAACAAGACACTGGCAACCACCGTGCCCTGCAAACGATTTTCCTG‑TAAAGTGCGCTTTTTGGAAGCCAACGCCAAGGCGGGGATATCCTTCATGGTTAAATGGGTGGTCGGGTTGTGCTGTTGTATATAAATGccaa                                                                                                                                                                           >  1:220091/1‑249 (MQ=255)                                                  aTGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATATGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCggg                                                                                                                                                                                                            <  1:673398/215‑1 (MQ=255)                                                      cATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATATGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCAcccc                                                                                                                                                                    <  1:549221/251‑4 (MQ=255)                                                      cATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATATGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCAcccc                                                                                                                                                                    <  1:850484/251‑4 (MQ=255)                                                      cATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGGGTCAATGTCGTCCTGAAAAGGCGGGTTGGGAAAGATCAGATCGAATTTTTCGTCAATATGGGAAAAAACGTCACTGGCAACCACCGCGCCTTGCAAACGATTTTTCTG‑TTAAGTGCGCTTTTCGGGAGCCAAAGCCAAGGCGGGAATATCCGTCATGATTAATCGGGGGTTCGGGGTGTGCTGTTGAATATAAATGCCAATCAccc                                                                                                                                                                     >  1:479260/1‑248 (MQ=255)                                                                gTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGGGTCAATGCCGTCATGAAAAGGCGGGTTGGGAAAGATCAGATCGAATTTGCCGTCAATATGGGGAAACACGTCCCTGGCAAACACCCTGCCTTGCAACCGATTTTTCTG‑TAAAGGGCGCTTTGCGGAAGCCAACGCCAAGGCGGGAATATCCGTCCTGATTAATCGGGTGTTCGGGGTGTGCTGTTGGatat                                                                                                                                                                                    >  1:316686/1‑225 (MQ=255)                                                                gTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGGGTCAAAGCCGTCATGAAAAAGCGGGTTGGAAAAGAACAAATCGAATTTGCCGTCAAAAAGGGGAAAAACGTCACTGGCAAACACCGTGCCCTGCAACCGATTTTTCTG‑TAAAGAGCGCTTTGCGGAAGCCAACGCCCAGGCGTGAATATCCGGCATGATTAATCGGGGGTTCGGGGTGGGCTGTTGAATATAAATGCCAATCAACCCTGCGCCGCAg                                                                                                                                                          >  1:482006/1‑251 (MQ=255)                                                                   ttATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATATGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGccc                                                                                                                                                       <  1:358242/251‑1 (MQ=255)                                                                    tATCAGTTCGTTAACGGCGGTGTAAGCGGGGTCAATGCCGTCCTGAAAAAGCGGGTTGGGAATGATCAGATCGAATTTTCCGTCAATATTGGGAAACACGTCACTGGCAACCACCCTGCCCTGCAACCGATTTTTCTT‑TAAAATGCGCTTTTCGGGAGCCAACGCCCAGGCGGGGATATCCCTCCTGATTAATCTGGGGTTCGGGGTTTGCTGTTGAATATAAATGCCCATCACCCCCGcgcccccgccc                                                                                                                                                       >  1:140971/1‑243 (MQ=255)                                                                         gTTCGTTAACGGCGGTGTAAGCGGGGTCAATGCCGTCATGAAAAAGCGGATTGGGAATGATCAGATCGAATTTGCCGTCCATATGGGGAAACACGTCACTGGCAACCACCCTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGGAGCCAACGCCCAGGCGTGAATATCCGTCATGATTAATCGGGGGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCCGCGCCGCAGCCCCCGTc                                                                                                                                                  >  1:849997/1‑250 (MQ=255)                                                                              ttAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATATGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAacac                                                                                                                                            <  1:362688/251‑1 (MQ=255)                                                                              ttAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATATGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAacac                                                                                                                                            <  1:86979/251‑1 (MQ=255)                                                                                  cggcggTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATATGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTg                                                                                                                                                                               >  1:323483/1‑212 (MQ=255)                                                                                       gtgtAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATATGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCggg                                                                                                                                                                                                            >  1:379572/1‑178 (MQ=255)                                                                                        tgtAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATATGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCAcccc                                                                                                                                                                    <  1:440843/217‑4 (MQ=255)                                                                                         gtAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATATGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAacac                                                                                                                                            >  1:589367/1‑240 (MQ=255)                                                                                                gtgtCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATATGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGttttt                                                                                                                          >  1:274544/1‑251 (MQ=255)                                                                                                  gtCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATATGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATg                                                                                                                                                                                                                      <  1:525035/157‑1 (MQ=255)                                                                                                  gtCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATATGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGAtt                                                                                                                                                                                                                   >  1:877830/1‑160 (MQ=255)                                                                                                           gtcatGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATATGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGaaa                                                                                                               >  1:534545/2‑251 (MQ=255)                                                                                                           gtcatGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATATGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGaaa                                                                                                               >  1:108366/2‑251 (MQ=255)                                                                                                             catGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATATGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGc                                                                                                             <  1:764399/251‑1 (MQ=255)                                                                                                               tGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATATGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTgcgc                                                                                                                                                               <  1:791792/199‑1 (MQ=255)                                                                                                                 aaaaggcggATTGGAAATGATCAGATCGAATTTGCCGTCAATATGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGCaat                                                                                                          <  1:278914/246‑1 (MQ=255)                                                                                                                      gcggATTGGAAATGATCAGATCGAATTTGCCGTCAATATGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGCAATAAtgc                                                                                                     <  1:142918/250‑1 (MQ=255)                                                                                                                         gattGGAAATGATCAGATCGAATTTGCCGTCAATATGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAatat                                                                                                                                                                                    >  1:283830/3‑168 (MQ=255)                                                                                                                         gattGGAAATGATCAGATCGAATTTGCCGTCAATATGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGCAATAAtgccgtg                                                                                                 >  1:93684/3‑251 (MQ=255)                                                                                                                             ggAAATGATCAGATCGAATTTGCCGTCAATATGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGtttt                                                                                                                           >  1:436831/1‑221 (MQ=255)                                                                                                                              gAAATGATCAGATCGAATTTGCCGTCAATATGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGCAATAATGCCGTgccgc                                                                                             >  1:619016/1‑250 (MQ=255)                                                                                                                                  tGATCAGATCGAATTTGCCGTCAATATGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGCAATAATGCCGTGCCGCCATcc                                                                                        <  1:706324/251‑1 (MQ=255)                                                                                                                                  tGATCAGATCGAATTTGCCGTCAATATGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGCAATAATGCCGTGCCGCCATcc                                                                                        <  1:893288/251‑1 (MQ=255)                                                                                                                                             aaTTTGCCGTCAATATGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGCAATAATGCCGTGCCGCCATCCAGCTcggcgg                                                                              <  1:38442/250‑1 (MQ=255)                                                                                                                                                    cGTCAATATGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTg                                                                                                                                                                                         >  1:848212/1‑136 (MQ=255)                                                                                                                                                      tCAATATGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGCAATAATGCCGTGCCGCCATCCAGCTCGGCGGCGCTGAATAc                                                                    <  1:663360/251‑1 (MQ=255)                                                                                                                                                       cAATATGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGCAATAATGCCGTGCCGCCATCCAGCTCGGCGGCGCTGAATACg                                                                   >  1:843908/1‑251 (MQ=255)                                                                                                                                                              ggAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGCAATAATGCCGTGCCGCCATCCAGCTCGGCGGCGCTGAATAc                                                                    >  1:360564/1‑243 (MQ=255)                                                                                                                                                                      cgtCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGc                                                                                                                                                                                              >  1:828982/3‑113 (MQ=255)                                                                                                                                                                        tCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGCAATAATGCCGTGCCGCCATCCAGCTCGGCGGCGCTGAATACGCCGGGCAACGCatagat                                                  <  1:344180/251‑1 (MQ=255)                                                                                                                                                                           cTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAatat                                                                                                                                                                                    <  1:357724/118‑1 (MQ=255)                                                                                                                                                                             ggCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACAcct                                                                                                                                          >  1:308843/1‑157 (MQ=255)                                                                                                                                                                             ggCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGtttttt                                                                                                                         <  1:865054/175‑1 (MQ=255)                                                                                                                                                                             ggCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGCAATAATGCCGTGCCGCCATCCAGCTCGGCGGCGCTGAATACGCCGGGCAACGCatagat                                                  <  1:485938/246‑1 (MQ=255)                                                                                                                                                                               caaccaccgtgccttgcaaccGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGCAATAATGCCGTGCCGCCATCCAGCTCGGCGGCGCTGAATACGCCGGGCAACGCATAGATAGTTaa                                            <  1:125533/231‑1 (MQ=255)                                                                                                                                                                                 accaccgtgccttgcaaccGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGCAATAATGCCGTGCCGCCATCCAGCTCGGCGGCGCTGAATACGCCGGGCAACGCatagat                                                  >  1:197010/18‑242 (MQ=255)                                                                                                                                                                                  ccaccgtgccttgcaaccGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGCAATAATGCCGTGCCGCCATCCAGCTCGGCGGCGCTGAATACGCCGGGCAACGCATAGATAGTTAAATCg                                        >  1:741085/17‑251 (MQ=255)                                                                                                                                                                                       gtgccttgcaaccGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGCAATAATGCCGTGCCGCCATCCAGCTCGGCGGCGCTGAATACGCCGGGCAACGc                                                        >  1:489031/12‑230 (MQ=255)                                                                                                                                                                                         gccttgcaaccGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGCAATAATGCCGTGCCGCCATCCAGCTCGGCGGCGCTGAATACGCCGGGCAACGCATAGATAGTTAAATCGGCaagtt                                 >  1:583796/10‑248 (MQ=255)                                                                                                                                                                                              gcaaccGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAg                                                                                                                       >  1:304257/5‑160 (MQ=255)                                                                                                                                                                                                    gATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGCAATAATGCCGTGCCGCCATCCAGCTCGGCGGCGCTGAATACGCCGGGCAACGCATAGATAGTTAAATCGGCAAGTTGCGACGATTGa                      >  1:392892/1‑251 (MQ=255)                                                                                                                                                                                                     aTTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGCAATAATGCCGTGCCGCCATCCAGCTCGGCGGCGCTGAATACGCCGGGCAACGCATAGATAGTTAAATCGGCAAGTTGCGACGATTGAt                     <  1:646635/251‑1 (MQ=255)                                                                                                                                                                                                         tCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGCAATAATGCCGTGCCGCCATCCAGCTCGGCGGCGCTGAATACGCCGGGCAACGCATAGATAGTTAAATCGGCAAGTTGCGACGATTGATAGGa                 <  1:555053/251‑1 (MQ=255)                                                                                                                                                                                                          ccTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGCAATAATGCCGTGCCGCCATCCAGCTCGGCGGCGCTGAATACGCCGGGCAACGCATAGATAGTTAAATCGGCAAGTTGCGACGATTGATAGGAt                >  1:544524/1‑251 (MQ=255)                                                                                                                                                                                                                aaaGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGCAATAATGCCGTGCCGCCATCCAGCTCGGCGGCGCTGAATACGCCGGGCAACGCatagat                                                  >  1:50987/1‑212 (MQ=255)                                                                                                                                                                                                                aaaGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGCAATAATGCCGTGCCGCCATCCAGCTCGGCGGCGCTGAATACGCCGGGCAACGCATAGATAGTTAAATCGGCAAGTTGCGACGATTGATAGGATTTCCa           >  1:252635/1‑251 (MQ=255)                                                                                                                                                                                                                          ttGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGCAATAAtgc                                                                                                     <  1:369104/151‑1 (MQ=255)                                                                                                                                                                                                                          ttGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGCAATAATGCCGTGCCGCCATCCAGCTCGGCGGCGCTGAATACGCCGGGCAACGCATAGATAGTTAAATCGGCAAGTTGCGACGATTGATAGGATTTCCAGAAAGtttt  >  1:857387/1‑250 (MQ=255)                                                                                                                                                                                                                                 aGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGCAATAATGCCGTGCCGCCATCCAGCTCGGCGGCGCTGAATACGCCGGGCAACGCatagat                                                  >  1:778693/1‑195 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                    AAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCATCATGAAACGGCGGGTTGGAAATGATCAGATCAAATTTGCCGTCAATATGGGAAAACACATCACTGGCAACCACTGTTCCCTGTAGCCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCATGAATATCTGTCATGATTAATCGGGTGTTCGGGTTGTGCCGTTGAATATAACTGCCGATCACTCCCGCGCCGCAGCCCACGTCCAACACCCCGCCTGCGATGTTTTTTAGCGTAGAAAGCAATAATGCCGTGCCGCCATCCAGTTCGGCGGCGCTGAATACGCCGGGCAACGCATAGATAGTTAAATCGGCAATTTGTGGCGATTGATAGGATTTCCAGAAAGTTTT  >  1/1828458‑1828923

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑