Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,828,233	T→C	intergenic (+46333/+7151)	unknown → / ← unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,828,233	0	T	C	100.0%	200.2 / NA	66	intergenic (+46333/+7151)	unknown/unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/39);  total (27/39)

TTTGAAGGGAGTGGAAAGACTGAAGAAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACTTTATTCATCGTGCCGCAACCGAAAATAATCTGATATTGTCCTGCTACGGCAAACTGCACTTTTACACCCTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTTGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTTCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGGCGATTTTAAGAAGATTCATATTATTCGCCGGAAGTGTCTTAAATAATAAGTAGAATTCTAACTAAAAGGCTTCGCTAAGCCAGCGGAATTTCGTGCACACTTAACTCATCCGTGCACCGAATAAACCTTAAACTTGTTATTTCTCGCCAGGACGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGC  >  1/1828000‑1828476                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                    tttGAAGGGAGTGGAAAGACTGAAGGAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGtttt                                                                                                                                                                                                                                    >  1:702419/1‑251 (MQ=255)        ggAGTGGAAAGACTGAAGGAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATaa                                                                                                                                                                                                                             >  1:677390/1‑251 (MQ=255)                       aaggaaaTAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGAttt                                                                                                                                                                                                              <  1:151209/247‑1 (MQ=255)                            aaTAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTaaga                                                                                                                                                                                                         <  1:383584/251‑1 (MQ=255)                            aaTAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTaaga                                                                                                                                                                                                         <  1:142420/251‑1 (MQ=255)                                      gcagcaTACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACGCCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGa                                                                                                                                                                                                                 <  1:791399/233‑1 (MQ=255)                                          caTACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCAtatt                                                                                                                                                                                              <  1:695531/248‑1 (MQ=255)                                            tACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCATATTATTCg                                                                                                                                                                                         >  1:149741/1‑251 (MQ=255)                                                   ccATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCATATTATTCGCCGGAAg                                                                                                                                                                                  <  1:303686/251‑1 (MQ=255)                                                   ccATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCATATTATTCGCCGGAAg                                                                                                                                                                                  <  1:869287/251‑1 (MQ=255)                                                    cATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCATATTATTCGCCGGAAgt                                                                                                                                                                                 >  1:67364/1‑251 (MQ=255)                                                             aaTTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCATATTATTCGCCGGAAGTGTCTTAaat                                                                                                                                                                        <  1:237041/251‑1 (MQ=255)                                                             aaTTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCATATTATTCGCCGGAAGTGTCTTAaat                                                                                                                                                                        <  1:795451/251‑1 (MQ=255)                                                                    cGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCATATTATTCGCCGGAAGTGTCTTAAATAATAAGt                                                                                                                                                                 <  1:710246/251‑1 (MQ=255)                                                                         accttATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTaaga                                                                                                                                                                                                         <  1:304805/203‑1 (MQ=255)                                                                           cttATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCATATTATTCGCCGGAAGTGTCTTAAATAATAAGTAGAATTc                                                                                                                                                          <  1:111413/250‑1 (MQ=255)                                                                              aTTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCATATTATTCGCCGGAAGTGTCTTAAATAATAAGTAGAATTctaa                                                                                                                                                       >  1:203718/1‑251 (MQ=255)                                                                                tCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCaa                                                                                                                                                                                                                                <  1:860712/176‑1 (MQ=255)                                                                                                 aaaaTAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCATATTATTCGCCGGAAGTGTCTTAAATAATAAGTAGAATTCTAACTAAAAGGCTTCGCTAAg                                                                                                                                     >  1:835646/1‑250 (MQ=255)                                                                                                  aaaTAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCATATTATTCGCCGGAAGTGTCTTAAATAATAAGTAGAAt                                                                                                                                                            >  1:788255/1‑226 (MQ=255)                                                                                                  aaaTAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCATATTATTCGCCGGAAGTGTCTTAAATAATAAGTAGAATTCTAACTAAAAGGCTTCGCTAAGcc                                                                                                                                   <  1:547760/251‑1 (MQ=255)                                                                                                  aaaTAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCATATTATTCGCCGGAAGTGTCTTAAATAATAAGTAGAATTCTAACTAAAAGGCTTCGCTAAGcc                                                                                                                                   <  1:449686/251‑1 (MQ=255)                                                                                                       atCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCATATTATTCGCCGGAAGTGTCTTAaat                                                                                                                                                                        <  1:530129/209‑1 (MQ=255)                                                                                                       atCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCATATTATTCGCCGGAAGTGTCTTAAATAATAAGTAGAATTCTAACTAAAAGGCTTCGCTAAGCCAGCgg                                                                                                                              >  1:816334/1‑251 (MQ=255)                                                                                                            atactGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCATATTATTCGCCGGAAGTGTCTTAaat                                                                                                                                                                        <  1:773625/200‑1 (MQ=255)                                                                                                                 gTCCTGCTACGGCAAATTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCATATTATTCGCCGGAAGTGTCTTAAATAATAAGTAGAATTctaact                                                                                                                                                     <  1:368371/218‑1 (MQ=255)                                                                                                                 gTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCATATTATTCGCCGGAAGTGTCTTAAATAATAAGTAGAATTctaact                                                                                                                                                     <  1:750162/218‑1 (MQ=255)                                                                                                                 gTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCATATTATTCGCCGGAAGTGTCTTAAATAATAAGTAGAATTCTAACTAAAAGGCTTCGCTAAGCCAGCGGAATTTCGTg                                                                                                                     >  1:342508/1‑250 (MQ=255)                                                                                                                         aCGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTaaga                                                                                                                                                                                                         <  1:606588/158‑1 (MQ=255)                                                                                                                            gCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCATATTATTCGCCGGAAGTGTCTTAAATAATAAGTAGAATTCTAACTAAAAGGCTTCGCTAAGCCAGCGGAATTTCGTGCACACTTAACt                                                                                                          <  1:854517/250‑1 (MQ=255)                                                                                                                             cAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCATATTATTCGCCGGAAGTGTCTTAAATAATAAGTAGAATTCTAACTAAAAGGCTTCGCTAAGCCAGCGGAATTTCGTGCACACTTAACTc                                                                                                         <  1:66957/250‑1 (MQ=255)                                                                                                                              aaaCTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCATATTATTCGCCGGAAGTGTCTTAAATAATAAGTAGAATTCTAACTAAAAGGCTTCGCTAAGCCAGCGGAATTTCGTGCACACTTAACTCAt                                                                                                       >  1:798346/1‑251 (MQ=255)                                                                                                                                 cTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGa                                                                                                                                                                                                                 <  1:339294/142‑1 (MQ=255)                                                                                                                                  tGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCATATTATTCGCCGGAAGTGTCTTAAATAATAAGTAGAATTCTAACTAAAAGGCTTCGCTAAGCCAGCGGAATTTCGTGCACACTTAACTCATctgc                                                                                                   <  1:892912/251‑4 (MQ=255)                                                                                                                                      cTTTTACACCTTCAATGTTGTTAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCATATTATTCGCCGGAAGTGTCTTAaataa                                                                                                                                                                      >  1:92332/1‑180 (MQ=255)                                                                                                                                      cTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCATATTATTCGCCGGAAGTGTCTTAAATAATAAGTAGAATTCTAACTAAAAGGCTTCGCTAAGCCAGCGGAATTTCGTGCACACTTAACTCATctgcgca                                                                                                >  1:326309/1‑244 (MQ=255)                                                                                                                                           acacCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCATATTATTCGCCGGAAGTGTCTTAAATAATAAGTAGAATTCTAACTAAAAGGCTTCGCTAAGCCAGCGGAATTTCGTGCACACTTAACTCATCTGCGCACCGAAt                                                                                          <  1:575442/251‑1 (MQ=255)                                                                                                                                              ccttCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCATATTATTCGCCGGAAGTGTCTTAAATAATAAGTAGAATTCTAACTAAAAGGCTTCGCTAAGcc                                                                                                                                   >  1:175395/4‑207 (MQ=255)                                                                                                                                               cttCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCATATTATTCGCCGGAAGTGTCTTAAATAATAAGTAGAATTCTAACTAAAAGGCTTCGCTAAGCCAGCGGAATTTCGTGCACACTTAACTCATCTGCGCACCGAATatac                                                                                      <  1:789987/249‑4 (MQ=255)                                                                                                                                                ttCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGa                                                                                                                                                                                                                 <  1:22498/126‑1 (MQ=255)                                                                                                                                                         gtCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCATATTATTCGCCGGAAGTGTCTTAaat                                                                                                                                                                        <  1:513877/159‑1 (MQ=255)                                                                                                                                                         gtCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCATATTATTCGCCGGAAGTGTCTTAAATAATAAGTAGAATTCTAACTAAAAGGCTTCGCTAAGCCAGCGGAATTTCGTGCACACTTAACTCATCTGCGCACCGAATat                                                                                        <  1:327243/239‑2 (MQ=255)                                                                                                                                                         gtCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCATATTATTCGCCGGAAGTGTCTTAAATAATAAGTAGAATTCTAACTAAAAGGCTTCGCTAAGCCAGCGGAATTTCGTGCACACTTAACTCATCTGCGCACCGAATATACCTTAAACTTg                                                                            >  1:644851/1‑251 (MQ=255)                                                                                                                                                         gtCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCATATTATTCGCCGGAAGTGTCTTAAATAATAAGTAGAATTCTAACTAAAAGGCTTCGCTAAGCCAGCGGAATTTCGTGCACACTTAACTCATCTGCGCACCGAATATACCTTAAACTTg                                                                            >  1:824137/1‑251 (MQ=255)                                                                                                                                                                    gTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCATATTATTCGCCGGAAGTGTCTTAAATAATAAGTAGAATTCTAACTAAAAGGCTTCGCTAAGCCAGCGGAAttt                                                                                                                         <  1:866341/195‑1 (MQ=255)                                                                                                                                                                                 tcgTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCATATTATTCGCCGGAAGTGTCTTAAATAATAAGTAGAATTCTAACTAAAAGGCTTCGCTAAGCCAGCGGAATTTCGTGCACACTTAACTCATCTGCGCACCGAATATACCTTAAACTTGTTATTTCTCGCCAGGACGTCATGa                                                    >  1:849002/3‑251 (MQ=255)                                                                                                                                                                                    ttATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCATATTATTCGCCGGAAGTGTCTTAaat                                                                                                                                                                        <  1:412062/132‑1 (MQ=255)                                                                                                                                                                                      aTTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCATATTATTCGCCGGAAGTGTCTTAAATAATAAGTAGAAt                                                                                                                                                            >  1:175586/1‑142 (MQ=255)                                                                                                                                                                                      aTTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCATATTATTCGCCGGAAGTGTCTTAAATAATAAGTAGAATTCTAACTAAAAGGCTTCGCTAAGCCAGCGGAATTTCGTGCACACTTAACTCATCTGCGCACCGAATATAcc                                                                                     <  1:631294/213‑1 (MQ=255)                                                                                                                                                                                      aTTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCATATTATTCGCCGGAAGTGTCTTAAATAATAAGTAGAATTCTAACTAAAAGGCTTCGCTAAGCCAGCGGAATTTCGTGCACACTTAACTCATCTGCGCACCGAATATACCTTAAACTTGTTATTTCTCGCCAGGACGTCATGAc                                                   >  1:331687/1‑247 (MQ=255)                                                                                                                                                                                            aacaacGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCATATTATTCGCCGGAAGTGTCTTAAATAATAAGTAGAATTCTAACTAAAAGGCTTCGCTAAGCCAGCGGAATTTCGTGCACACTTAACTCATCTGCGCACCGAATATACCTTAAACTTGTTATTTCTCGCCAGGACGTCATGACTGCCGaaa                                           <  1:55707/249‑1 (MQ=255)                                                                                                                                                                                                   cgcCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCATATTATTCGCCGGAAGTGTCt                                                                                                                                                                             <  1:243083/112‑1 (MQ=255)                                                                                                                                                                                                   cgcCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCATATTATTCGCCGGAAGTGTCTTAAATAATAAGTAGAATTCTAACTAAAAGGCTTCGCTAAGCCAGCGGAATTTCGTGCACACTTAACTCATCTGCGCACCGAATATACCTTAAACTTGTTATTTCTCGCCAGGACGTCATGACTGCCGAAATGTTGAtt                                   >  1:556395/1‑250 (MQ=255)                                                                                                                                                                                                     ggctgcacaggCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCATATTATTCGCCGGAAGTGTCTTAAATAATAAGTAGAATTCTAACTAAAAGGCTTCGCTAAGCCAGCGGAATTTCGTGCACACTTAACTCATCTGCGCACCGAATATACCTTAAACTTGTTATTTCTCGCCAGGACGTCATGACTGCCGAAATGTTGATTCAg                                >  1:836921/10‑251 (MQ=255)                                                                                                                                                                                                                 gcAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCATATTATTCGCCGGAAGTGTCTTAAATAATAAGTAGAATTCTAACTAAAAGGCTTCGCTAAGCCAGCGGAATTTCGTGCACACTTAACTCATc                                                                                                      <  1:136634/169‑1 (MQ=255)                                                                                                                                                                                                                    gTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCATATTATTCGCCGGAAGTGTCTTAAATAATAAGTAGAATTCTAACTAAAAGGCTTCGCTAAGCCAGCGGAATTTCGTGCACACTTAACTCATCTGCGCACCGAATATACCTTAAACt                                                                              <  1:325815/190‑1 (MQ=255)                                                                                                                                                                                                                    gTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCATATTATTCGCCGGAAGTGTCTTAAATAATAAGTAGAATTCTAACTAAAAGGCTTCGCTAAGCCAGCGGAATTTCGTGCACACTTAACTCATCTGCGCACCGAATATACCTTAAACTTGTTATTTCTCGCCAGGACGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAgg                 >  1:140673/1‑251 (MQ=255)                                                                                                                                                                                                                     tGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCATATTATTCGCCGGAAGTGTCTTAAATAATAAGTAGAATTCTAACTAAAAGGCTTCGCTAAGCCAGCGGAATTTCGTGCACACTTAACTCATCTGCGCACCGAATATACCTTAAACTTGTTATTTCTCGCCAGGACGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGc                <  1:518554/251‑1 (MQ=255)                                                                                                                                                                                                                     tGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCATATTATTCGCCGGAAGTGTCTTAAATAATAAGTAGAATTCTAACTAAAAGGCTTCGCTAAGCCAGCGGAATTTCGTGCACACTTAACTCATCTGCGCACCGAATATACCTTAAACTTGTTATTTCTCGCCAGGACGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGc                <  1:404751/251‑1 (MQ=255)                                                                                                                                                                                                                      gggTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCatat                                                                                                                                                                                               <  1:674097/75‑1 (MQ=255)                                                                                                                                                                                                                              gTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCATATTATTCGCCGGAAGTGTCTTAAATAATAAGTAGAATTCTAACTAAAAGGCTTCGCTAAGCCAGCGGAATTTCGTGCACACTAAACTCATCTGCGCACCGAATATACCTTAAACTTGTTATTTCTCGCCAGGACGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCa       >  1:288100/1‑251 (MQ=255)                                                                                                                                                                                                                                  caATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCATATTATTCGCCGGAAGTGTCTTAAATAATAAGTAGAATTCTAACTAAAAGGCTTCGCTAAGCCAGCGGAATTTCGTGCACACTTAACTCATCTGCGCACCGAATATACCTTAAACTTGTTATTTCTCGCCAGGACGTCATGACTGCCGaaa                                           >  1:645939/1‑211 (MQ=255)                                                                                                                                                                                                                                  caATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCATATTATTCGCCGGAAGTGTCTTAAATAATAAGTAGAATTCTAACTAAAAGGCTTCGCTAAGCCAGCGGAATTTCGTGCACACTTAACTCATCTGCGCACCGAATATACCTTAAACTTGTTATTTCTCGCCAGGACGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTg   >  1:680419/1‑251 (MQ=255)                                                                                                                                                                                                                                  caATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCATATTATTCGCCGGAAGTGTCTTAAATAATAAGTAGAATTCTAACTAAAAGGCTTCGCTAAGCCAGCGGAATTTCGTGCACACTTAACTCATCTGCGCACCGAATATACCTTAAACTTGTTATTTCTCGCCAGGACGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTg   >  1:269025/1‑251 (MQ=255)                                                                                                                                                                                                                                    atatTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCATATTATTCGCCGGAAGTGTCTTAAATAATAAGTAGAATTCTAACTAAAAGGCTTCGCTAAGCCAGCGGAATTTCGTGCACACTTAACTCATCTGCGCACCGAATATACCTTAAACTTGTTATTTCTCGCCAGGACGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGc  >  1:61378/1‑250 (MQ=255)                                                                                                                                                                                                                                      atTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCATATTATTCGCCGGAAGTGTCTTAAATAATAAGTAGAATTCTAACTAAAAGGCTTCGCTAAGCCAGCGGAAttt                                                                                                                         >  1:344417/1‑129 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                    TTTGAAGGGAGTGGAAAGACTGAAGAAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACTTTATTCATCGTGCCGCAACCGAAAATAATCTGATATTGTCCTGCTACGGCAAACTGCACTTTTACACCCTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTTGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTTCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGGCGATTTTAAGAAGATTCATATTATTCGCCGGAAGTGTCTTAAATAATAAGTAGAATTCTAACTAAAAGGCTTCGCTAAGCCAGCGGAATTTCGTGCACACTTAACTCATCCGTGCACCGAATAAACCTTAAACTTGTTATTTCTCGCCAGGACGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGC  >  1/1828000‑1828476

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑