Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,828,177	T→C	intergenic (+46277/+7207)	unknown → / ← unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,828,177	0	T	C	100.0%	195.5 / NA	68	intergenic (+46277/+7207)	unknown/unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (30/38);  total (30/38)

AGTACCAACCTGTTTGCAGATTCGCGCTTTAATGGAACAAATCTAAATCACATTTTTAGCATTTGAAGGGAGTGGAAAGACTGAAGAAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACTTTATTCATCGTGCCGCAACCGAAAATAATCTGATATTGTCCTGCTACGGCAAACTGCACTTTTACACCCTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTTGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTTCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGGCGATTTTAAGAAGATTCATATTATTCGCCGGAAGTGTCTTAAATAATAAGTAGAATTCTAACTAAAAGGCTTCGCTAAGCCAGCGGAATTTCGTGCACACTTAACTCATCCGTGCACCGAATAAACCTTAAACTTG  >  1/1827939‑1828402                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                  aGTACCAACCTGTTTGCAGATTCGCGCTTTAATGGAACAAATCTAAATCACATTTTTAGCATTTGAAGGGAGTGGAAAGACTGAAGGAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGaa                                                                                                                                                                                                                        >  1:297441/1‑250 (MQ=255)   tgccAACCTGTTTGCAGATTCGCGCTTTAATGGAACAAATCTAAATCACATTTTTAGCATTTGAAGGGAGTGGAAAGACTGAAGGAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGaacaa                                                                                                                                                                                                                     <  1:320466/249‑1 (MQ=255)         ccTGTTTGCAGATTCGCGCTTTAATGGAACAAATCTAAATCACATTTTTAGCATTTGAAGGGAGTGGAAAGACTGAAGGAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACgcgc                                                                                                                                                                                                                >  1:239768/1‑250 (MQ=255)                 cAGATTCGCGCTTTAATGGAACAAATCTAAATCACATTTTTAGCATTTGAAGGGAGTGGAAAGACTGAAGGAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCAt                                                                                                                                                                                                        <  1:130361/250‑1 (MQ=255)                   gATTCGCGCTTTAATGGAACAAATCTAAATCACATTTTTAGCATTTGAAGGGAGTGGAAAGACTGAAGGAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATgg                                                                                                                                                                                                      >  1:132615/1‑250 (MQ=255)                    aTTCGCGCTTTAATGGAACAAATCTAAATCACATTTTTAGCATTTGAAGGGAGTGGAAAGACTGAAGGAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGgc                                                                                                                                                                                                     <  1:399774/250‑1 (MQ=255)                           cTTTAATGGAACAAATCTAAATCACATTTTTAGCATTTGAAGGGAGTGGAAAGACTGAAGGAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTggg                                                                                                                                                                                             >  1:740367/1‑251 (MQ=255)                           cTTTAATGGAACAAATCTAAATCACATTTTTAGCATTTGAAGGGAGTGGAAAGACTGAAGGAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTggg                                                                                                                                                                                             >  1:629246/1‑251 (MQ=255)                           cTTTAATGGAACAAATCTAAATCACATTTTTAGCATTTGAAGGGAGCGGAAAGACTGAAGGAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTggg                                                                                                                                                                                             >  1:441028/1‑251 (MQ=255)                             ttAATGGAACAAATCTAAATCACATTTTTAGCATTTGAAGGGAGTGGAAAGACTGAAGGAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTa                                                                                                                                                                                           >  1:802919/1‑251 (MQ=255)                              tAATGGAACAAATCTAAATCACATTTTTAGCATTTGAAGGGAGTGGAAAGACTGAAGGAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGt                                                                                                                                                                                            <  1:676829/249‑1 (MQ=255)                                   gAACAAATCTAAATCACATTTTTAGCATTTGAAGGGAGTGGAAAGACTGAAGGAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGaac                                                                                                                                                                                                                       >  1:215402/1‑217 (MQ=255)                                          tCTAAATCACATTTTTAGCATTTGAAGGGAGTGGAAAGACTGAAGGAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAatat                                                                                                                                                                              <  1:50502/251‑1 (MQ=255)                                                              tttGAAGGGAGTGGAAAGACTGAAGGAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGgcgc                                                                                                                                                                                                   <  1:834626/210‑1 (MQ=255)                                                              tttGAAGGGAGTGGAAAGACTGAAGGAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGtttt                                                                                                                                                          >  1:702419/1‑251 (MQ=255)                                                                    gggAGTGGAAAGACTGAAGGAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTg                                                                                                                                                                                       >  1:191264/1‑216 (MQ=255)                                                                     ggAGTGGAAAGACTGAAGGAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATaa                                                                                                                                                   >  1:677390/1‑251 (MQ=255)                                                                         tGGAAAGACTGAAGGAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGgc                                                                                                                                                                                                     <  1:95511/197‑1 (MQ=255)                                                                              aGACTGAAGGAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATAtttcct                                                                                                                                                                         >  1:26748/1‑217 (MQ=255)                                                                              aGACTGAAGGAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATAtttc                                                                                                                                                                           >  1:627086/1‑217 (MQ=255)                                                                                    aaggaaaTAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGAttt                                                                                                                                    <  1:151209/247‑1 (MQ=255)                                                                                         aaTAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTaaga                                                                                                                               <  1:142420/251‑1 (MQ=255)                                                                                         aaTAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTaaga                                                                                                                               <  1:383584/251‑1 (MQ=255)                                                                                            aaagaaagCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATAtttcct                                                                                                                                                                         >  1:735056/1‑203 (MQ=255)                                                                                                   gcagcaTACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACGCCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGa                                                                                                                                       <  1:791399/233‑1 (MQ=255)                                                                                                       caTACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCAtatt                                                                                                                    <  1:695531/248‑1 (MQ=255)                                                                                                         tACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCATATTATTCg                                                                                                               >  1:149741/1‑251 (MQ=255)                                                                                                                ccATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCATATTATTCGCCGGAAg                                                                                                        <  1:303686/251‑1 (MQ=255)                                                                                                                ccATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCATATTATTCGCCGGAAg                                                                                                        <  1:869287/251‑1 (MQ=255)                                                                                                                 cATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCATATTATTCGCCGGAAgt                                                                                                       >  1:67364/1‑251 (MQ=255)                                                                                                                          aaTTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCATATTATTCGCCGGAAGTGTCTTAaat                                                                                              <  1:237041/251‑1 (MQ=255)                                                                                                                          aaTTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCATATTATTCGCCGGAAGTGTCTTAaat                                                                                              <  1:795451/251‑1 (MQ=255)                                                                                                                                 cGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCATATTATTCGCCGGAAGTGTCTTAAATAATAAGt                                                                                       <  1:710246/251‑1 (MQ=255)                                                                                                                                     gaccttATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAat                                                                                                                                                                                >  1:87765/5‑158 (MQ=255)                                                                                                                                      accttATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTcac                                                                                                                                                                                   >  1:17084/4‑154 (MQ=255)                                                                                                                                      accttATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTaaga                                                                                                                               <  1:304805/203‑1 (MQ=255)                                                                                                                                        cttATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCATATTATTCGCCGGAAGTGTCTTAAATAATAAGTAGAATTc                                                                                <  1:111413/250‑1 (MQ=255)                                                                                                                                           aTTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCATATTATTCGCCGGAAGTGTCTTAAATAATAAGTAGAATTctaa                                                                             >  1:203718/1‑251 (MQ=255)                                                                                                                                             tCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCaa                                                                                                                                                      <  1:860712/176‑1 (MQ=255)                                                                                                                                                              aaaaTAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCATATTATTCGCCGGAAGTGTCTTAAATAATAAGTAGAATTCTAACTAAAAGGCTTCGCTAAg                                                           >  1:835646/1‑250 (MQ=255)                                                                                                                                                               aaaTAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCATATTATTCGCCGGAAGTGTCTTAAATAATAAGTAGAAt                                                                                  >  1:788255/1‑226 (MQ=255)                                                                                                                                                               aaaTAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCATATTATTCGCCGGAAGTGTCTTAAATAATAAGTAGAATTCTAACTAAAAGGCTTCGCTAAGcc                                                         <  1:547760/251‑1 (MQ=255)                                                                                                                                                               aaaTAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCATATTATTCGCCGGAAGTGTCTTAAATAATAAGTAGAATTCTAACTAAAAGGCTTCGCTAAGcc                                                         <  1:449686/251‑1 (MQ=255)                                                                                                                                                                    atCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCATATTATTCGCCGGAAGTGTCTTAaat                                                                                              <  1:530129/209‑1 (MQ=255)                                                                                                                                                                    atCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCATATTATTCGCCGGAAGTGTCTTAAATAATAAGTAGAATTCTAACTAAAAGGCTTCGCTAAGCCAGCgg                                                    >  1:816334/1‑251 (MQ=255)                                                                                                                                                                         atactGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCATATTATTCGCCGGAAGTGTCTTAaat                                                                                              <  1:773625/200‑1 (MQ=255)                                                                                                                                                                              gTCCTGCTACGGCAAATTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCATATTATTCGCCGGAAGTGTCTTAAATAATAAGTAGAATTctaact                                                                           <  1:368371/218‑1 (MQ=255)                                                                                                                                                                              gTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCATATTATTCGCCGGAAGTGTCTTAAATAATAAGTAGAATTctaact                                                                           <  1:750162/218‑1 (MQ=255)                                                                                                                                                                              gTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCATATTATTCGCCGGAAGTGTCTTAAATAATAAGTAGAATTCTAACTAAAAGGCTTCGCTAAGCCAGCGGAATTTCGTg                                           >  1:342508/1‑250 (MQ=255)                                                                                                                                                                                      aCGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTaaga                                                                                                                               <  1:606588/158‑1 (MQ=255)                                                                                                                                                                                         gCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCATATTATTCGCCGGAAGTGTCTTAAATAATAAGTAGAATTCTAACTAAAAGGCTTCGCTAAGCCAGCGGAATTTCGTGCACACTTAACt                                <  1:854517/250‑1 (MQ=255)                                                                                                                                                                                          cAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCATATTATTCGCCGGAAGTGTCTTAAATAATAAGTAGAATTCTAACTAAAAGGCTTCGCTAAGCCAGCGGAATTTCGTGCACACTTAACTc                               <  1:66957/250‑1 (MQ=255)                                                                                                                                                                                           aaaCTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCATATTATTCGCCGGAAGTGTCTTAAATAATAAGTAGAATTCTAACTAAAAGGCTTCGCTAAGCCAGCGGAATTTCGTGCACACTTAACTCAt                             >  1:798346/1‑251 (MQ=255)                                                                                                                                                                                              cTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGa                                                                                                                                       <  1:339294/142‑1 (MQ=255)                                                                                                                                                                                               tGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCATATTATTCGCCGGAAGTGTCTTAAATAATAAGTAGAATTCTAACTAAAAGGCTTCGCTAAGCCAGCGGAATTTCGTGCACACTTAACTCATctgc                         <  1:892912/251‑4 (MQ=255)                                                                                                                                                                                                   cTTTTACACCTTCAATGTTGTTAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCATATTATTCGCCGGAAGTGTCTTAaataa                                                                                            >  1:92332/1‑180 (MQ=255)                                                                                                                                                                                                   cTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCATATTATTCGCCGGAAGTGTCTTAAATAATAAGTAGAATTCTAACTAAAAGGCTTCGCTAAGCCAGCGGAATTTCGTGCACACTTAACTCATctgcgca                      >  1:326309/1‑244 (MQ=255)                                                                                                                                                                                                     tttACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACaa                                                                                                                                                                                 <  1:118668/93‑1 (MQ=255)                                                                                                                                                                                                        acacCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCATATTATTCGCCGGAAGTGTCTTAAATAATAAGTAGAATTCTAACTAAAAGGCTTCGCTAAGCCAGCGGAATTTCGTGCACACTTAACTCATCTGCGCACCGAAt                <  1:575442/251‑1 (MQ=255)                                                                                                                                                                                                           ccttCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATAtttcc                                                                                                                                                                          >  1:588100/4‑92 (MQ=255)                                                                                                                                                                                                           ccttCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCATATTATTCGCCGGAAGTGTCTTAAATAATAAGTAGAATTCTAACTAAAAGGCTTCGCTAAGcc                                                         >  1:175395/4‑207 (MQ=255)                                                                                                                                                                                                            cttCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCATATTATTCGCCGGAAGTGTCTTAAATAATAAGTAGAATTCTAACTAAAAGGCTTCGCTAAGCCAGCGGAATTTCGTGCACACTTAACTCATCTGCGCACCGAATatac            <  1:789987/249‑4 (MQ=255)                                                                                                                                                                                                             ttCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGa                                                                                                                                       <  1:22498/126‑1 (MQ=255)                                                                                                                                                                                                                      gtCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCATATTATTCGCCGGAAGTGTCTTAaat                                                                                              <  1:513877/159‑1 (MQ=255)                                                                                                                                                                                                                      gtCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCATATTATTCGCCGGAAGTGTCTTAAATAATAAGTAGAATTCTAACTAAAAGGCTTCGCTAAGCCAGCGGAATTTCGTGCACACTTAACTCATCTGCGCACCGAATat              <  1:327243/239‑2 (MQ=255)                                                                                                                                                                                                                      gtCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCATATTATTCGCCGGAAGTGTCTTAAATAATAAGTAGAATTCTAACTAAAAGGCTTCGCTAAGCCAGCGGAATTTCGTGCACACTTAACTCATCTGCGCACCGAATATACCTTAAACTTg  >  1:824137/1‑251 (MQ=255)                                                                                                                                                                                                                      gtCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCATATTATTCGCCGGAAGTGTCTTAAATAATAAGTAGAATTCTAACTAAAAGGCTTCGCTAAGCCAGCGGAATTTCGTGCACACTTAACTCATCTGCGCACCGAATATACCTTAAACTTg  >  1:644851/1‑251 (MQ=255)                                                                                                                                                                                                                                 gTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCATATTATTCGCCGGAAGTGTCTTAAATAATAAGTAGAATTCTAACTAAAAGGCTTCGCTAAGCCAGCGGAAttt                                               <  1:866341/195‑1 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                  AGTACCAACCTGTTTGCAGATTCGCGCTTTAATGGAACAAATCTAAATCACATTTTTAGCATTTGAAGGGAGTGGAAAGACTGAAGAAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACTTTATTCATCGTGCCGCAACCGAAAATAATCTGATATTGTCCTGCTACGGCAAACTGCACTTTTACACCCTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTTGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTTCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGGCGATTTTAAGAAGATTCATATTATTCGCCGGAAGTGTCTTAAATAATAAGTAGAATTCTAACTAAAAGGCTTCGCTAAGCCAGCGGAATTTCGTGCACACTTAACTCATCCGTGCACCGAATAAACCTTAAACTTG  >  1/1827939‑1828402

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑