Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,828,110	T→C	intergenic (+46210/+7274)	unknown → / ← unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,828,110	0	T	C	100.0%	179.2 / NA	62	intergenic (+46210/+7274)	unknown/unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/33);  total (29/33)

TATTTCTGAGTAAGTGCGGAAAAGAATTTACACCAAGTGCGGTCGTCTTACAAAACGTTTTTCATTATCAGAAAAGTACCAACCTGTTTGCAGATTCGCGCTTTAATGGAACAAATCTAAATCACATTTTTAGCATTTGAAGGGAGTGGAAAGACTGAAGAAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACTTTATTCATCGTGCCGCAACCGAAAATAATCTGATATTGTCCTGCTACGGCAAACTGCACTTTTACACCCTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTTGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTTCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGGCGATTTTAAGAAGATTCATATTATTCGCCGGAAGTGTCTTAAATAATAAGTAGAATTCTAACTAAAAGGCTTCGCTAAGCCAGCGG  >  1/1827865‑1828352                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                   tATTTCTGAGTAAGTGCGGAAAAGAATTTACACCAAGTGCGGTCGTCTTACAAAACGTTTTTCATTATCAGAAAAGTACCAACCTGTTTGCAGATTCGCGCTTTAATGGAACAAATCTAAATCACATTTTTAGCATTTGAAGGGAGTGGAAAGACTGAAGGAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGGCCGTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTcc                                                                                                                                                                                                                                               <  1:242860/251‑1 (MQ=255)      tctaagtaatggcgAAAAAAAATTACACCAAGTGCGGTCGTCTTACAAAACGTTTTTCATTATCAGAAAAGTACCAACCTGTTTGCAGATTCGCGCTTTAATGGAACAAATCTAAATCACATTTTTAGCATTTGAAGGGAGTGGAAAGACTGAAGGAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTa                                                                                                                                                                                                                                          <  1:307425/238‑1 (MQ=255)                     aaaGAATTTACACCAAGTGCGGTCGTCTTACAAAACGTTTTTCATTATCAGAAAAGTACCAACCTGTTTGCAGATTCGCGCTTTAATGGAACAAATCTAAATCACATTTTTAGCATTTGAAGGGAGTGGAAAGACTGAAGGAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTc                                                                                                                                                                                                                                                >  1:90303/1‑230 (MQ=255)                         aaTTTACACCAAGTGCGGTCGTCTTACAAAACGTTTTTCATTATCAGAAAAGTACCAACCTGTTTGCAGATTCGCGCTTTAATGGAACAAATCTAAATCACATTTTTAGCATTTGAAGGGAGTGGAAAGACTGAAGGAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTa                                                                                                                                                                                                                        >  1:637919/1‑250 (MQ=255)                                   aaGTGCGGTCGTCTTACAAAACGTTTTTCATTATCAGAAAGGTGCCAACCTGTTTGCAGATTCGCGCTTTAATGGAACAAATCTAAATCACATTTTTAGCATTTGAAGGGAGTGGAAAGACTGAAGGAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAAtg                                                                                                                                                                                                             <  1:346536/251‑1 (MQ=255)                                   aaGTGCGGTCGTCTTACAAAACGTTTTTCATTATCAGAAAAGTACCAACCTGTTTGCAGATTCGCGCTTTAATGGAACAAATCTAAATCACATTTTTAGCATTTGAAGGGAGTGGAAAGACTGAAGGAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAAtg                                                                                                                                                                                                             >  1:796887/1‑251 (MQ=255)                                   aaGTGCGGTCGTCTTACAAAACGTTTTTCATTATCAGAAAAGTACCAACCTGTTTGCAGATTCGCGCTTTAATGGAACAAATCTAAATCACATTTTTAGCATTTGAAGGGAGTGGAAAGACTGAAGGAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAAtg                                                                                                                                                                                                             <  1:656002/251‑1 (MQ=255)                                   aaGTGCGGTCGTCTTACAAAACGTTTTTCATTATCAGAAAAGTACCAACCTGTTTGCAGATTCGCGCTTTAATGGAACAAATCTAAATCACATTTTTAGCATTTGAAGGGAGTGGAAAGACTGAAGGAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAAtg                                                                                                                                                                                                             <  1:182422/251‑1 (MQ=255)                                          gtcgtcTTACAAAACGTTTTTCATTATCAGAAAAGTACCAACCTGTTTGCAGATTCGCGCTTTAATGGAACAAATCTAAATCACATTTTTAGCATTTGAAGGGAGTGGAAAGACTGAAGGAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCa                                                                                                                                                                                                       <  1:802281/250‑1 (MQ=255)                                                      aaCGTTTTTCATTATCAGAAAAGTACCAACCTGTTTGCAGATTCGCGCTTTAATGGAACAAATCTAAATCACATTTTTAGCATTTGAAGGGAGTGGAAAGACTGAAGGAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAAc                                                                                                                                                                                          <  1:778064/251‑1 (MQ=255)                                                         gTTTTTCATTATCAGAAAAGTACCAACCTGTTTGCAGATTCGCGCTTTAATGGAACAAATCTAAATCACATTTTTAGCATTTGAAGGGAGTGGAAAGACTGAAGGAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACttt                                                                                                                                                                                       >  1:451681/1‑251 (MQ=255)                                                                   aTCAGAAAAGTACCAACCTGTTTGCAGATTCGCGCTTTAATGGAACAAATCTAAATCACATTTTTAGCATTTGAAGGGAGTGGAAAGACTGAAGGAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTac                                                                                                                                                                                                                       >  1:444507/1‑209 (MQ=255)                                                                           aGTACCAACCTGTTTGCAGATTCGCGCTTTAATGGAACAAATCTAAATCACATTTTTAGCATTTGAAGGGAGTGGAAAGACTGAAGGAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGaa                                                                                                                                                                      >  1:297441/1‑250 (MQ=255)                                                                             tgccAACCTGTTTGCAGATTCGCGCTTTAATGGAACAAATCTAAATCACATTTTTAGCATTTGAAGGGAGTGGAAAGACTGAAGGAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGaacaa                                                                                                                                                                   <  1:320466/249‑1 (MQ=255)                                                                                   ccTGTTTGCAGATTCGCGCTTTAATGGAACAAATCTAAATCACATTTTTAGCATTTGAAGGGAGTGGAAAGACTGAAGGAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACgcgc                                                                                                                                                              >  1:239768/1‑250 (MQ=255)                                                                                           cAGATTCGCGCTTTAATGGAACAAATCTAAATCACATTTTTAGCATTTGAAGGGAGTGGAAAGACTGAAGGAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCAt                                                                                                                                                      <  1:130361/250‑1 (MQ=255)                                                                                             gATTCGCGCTTTAATGGAACAAATCTAAATCACATTTTTAGCATTTGAAGGGAGTGGAAAGACTGAAGGAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATgg                                                                                                                                                    >  1:132615/1‑250 (MQ=255)                                                                                              aTTCGCGCTTTAATGGAACAAATCTAAATCACATTTTTAGCATTTGAAGGGAGTGGAAAGACTGAAGGAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGgc                                                                                                                                                   <  1:399774/250‑1 (MQ=255)                                                                                                     cTTTAATGGAACAAATCTAAATCACATTTTTAGCATTTGAAGGGAGTGGAAAGACTGAAGGAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTggg                                                                                                                                           >  1:629246/1‑251 (MQ=255)                                                                                                     cTTTAATGGAACAAATCTAAATCACATTTTTAGCATTTGAAGGGAGTGGAAAGACTGAAGGAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTggg                                                                                                                                           >  1:740367/1‑251 (MQ=255)                                                                                                     cTTTAATGGAACAAATCTAAATCACATTTTTAGCATTTGAAGGGAGCGGAAAGACTGAAGGAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTggg                                                                                                                                           >  1:441028/1‑251 (MQ=255)                                                                                                       ttAATGGAACAAATCTAAATCACATTTTTAGCATTTGAAGGGAGTGGAAAGACTGAAGGAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTa                                                                                                                                         >  1:802919/1‑251 (MQ=255)                                                                                                        tAATGGAACAAATCTAAATCACATTTTTAGCATTTGAAGGGAGTGGAAAGACTGAAGGAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGt                                                                                                                                          <  1:676829/249‑1 (MQ=255)                                                                                                             gAACAAATCTAAATCACATTTTTAGCATTTGAAGGGAGTGGAAAGACTGAAGGAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCtt                                                                                                                                                                                   >  1:651220/1‑203 (MQ=255)                                                                                                             gAACAAATCTAAATCACATTTTTAGCATTTGAAGGGAGTGGAAAGACTGAAGGAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGaac                                                                                                                                                                     >  1:215402/1‑217 (MQ=255)                                                                                                                  aaTCTAAATCACATTTTTAGCATTTGAAGGGAGTGGAAAGACTGAAGGAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTa                                                                                                                                                                                                                                          <  1:43997/143‑1 (MQ=255)                                                                                                                    tCTAAATCACATTTTTAGCATTTGAAGGGAGTGGAAAGACTGAAGGAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAatat                                                                                                                            <  1:50502/251‑1 (MQ=255)                                                                                                                                        tttGAAGGGAGTGGAAAGACTGAAGGAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACttt                                                                                                                                                                                       <  1:32886/172‑1 (MQ=255)                                                                                                                                        tttGAAGGGAGTGGAAAGACTGAAGGAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGgcgc                                                                                                                                                 <  1:834626/210‑1 (MQ=255)                                                                                                                                        tttGAAGGGAGTGGAAAGACTGAAGGAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGtttt                                                                                                        >  1:702419/1‑251 (MQ=255)                                                                                                                                              gggAGTGGAAAGACTGAAGGAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTg                                                                                                                                     >  1:191264/1‑216 (MQ=255)                                                                                                                                               ggAGTGGAAAGACTGAAGGAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATaa                                                                                                 >  1:677390/1‑251 (MQ=255)                                                                                                                                                   tGGAAAGACTGAAGGAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGgc                                                                                                                                                   <  1:95511/197‑1 (MQ=255)                                                                                                                                                    ggAAAGACAGAAGGAAATAAAGAAAGCAGCATACCGGGCTATTTAATCAATTCGGCGTGGACCTTATTCATCGTTACGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACCCCTTCAAtg                                                                                                                                                                                                             <  1:768614/138‑1 (MQ=255)                                                                                                                                                        aGACTGAAGGAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATAtttcct                                                                                                                       >  1:26748/1‑217 (MQ=255)                                                                                                                                                        aGACTGAAGGAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATAtttc                                                                                                                         >  1:627086/1‑217 (MQ=255)                                                                                                                                                              aaggaaaTAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGAttt                                                                                  <  1:151209/247‑1 (MQ=255)                                                                                                                                                                   aaTAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTaaga                                                                             <  1:383584/251‑1 (MQ=255)                                                                                                                                                                   aaTAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTaaga                                                                             <  1:142420/251‑1 (MQ=255)                                                                                                                                                                      aaagaaagCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATAtttcct                                                                                                                       >  1:735056/1‑203 (MQ=255)                                                                                                                                                                             gcagcaTACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACGCCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGa                                                                                     <  1:791399/233‑1 (MQ=255)                                                                                                                                                                                 caTACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCAtatt                                                                  <  1:695531/248‑1 (MQ=255)                                                                                                                                                                                   tACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCATATTATTCg                                                             >  1:149741/1‑251 (MQ=255)                                                                                                                                                                                          ccATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCATATTATTCGCCGGAAg                                                      <  1:303686/251‑1 (MQ=255)                                                                                                                                                                                          ccATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCATATTATTCGCCGGAAg                                                      <  1:869287/251‑1 (MQ=255)                                                                                                                                                                                           cATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCATATTATTCGCCGGAAgt                                                     >  1:67364/1‑251 (MQ=255)                                                                                                                                                                                                    aaTTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCATATTATTCGCCGGAAGTGTCTTAaat                                            <  1:795451/251‑1 (MQ=255)                                                                                                                                                                                                    aaTTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCATATTATTCGCCGGAAGTGTCTTAaat                                            <  1:237041/251‑1 (MQ=255)                                                                                                                                                                                                           cGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCATATTATTCGCCGGAAGTGTCTTAAATAATAAGt                                     <  1:710246/251‑1 (MQ=255)                                                                                                                                                                                                               gaccttATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAat                                                                                                                              >  1:87765/5‑158 (MQ=255)                                                                                                                                                                                                                accttATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGc                                                                                                                                                                                                   >  1:400115/4‑88 (MQ=255)                                                                                                                                                                                                                accttATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTcac                                                                                                                                 >  1:17084/4‑154 (MQ=255)                                                                                                                                                                                                                accttATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTaaga                                                                             <  1:304805/203‑1 (MQ=255)                                                                                                                                                                                                                  cttATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCATATTATTCGCCGGAAGTGTCTTAAATAATAAGTAGAATTc                              <  1:111413/250‑1 (MQ=255)                                                                                                                                                                                                                     aTTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCATATTATTCGCCGGAAGTGTCTTAAATAATAAGTAGAATTctaa                           >  1:203718/1‑251 (MQ=255)                                                                                                                                                                                                                       tCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCaa                                                                                                    <  1:860712/176‑1 (MQ=255)                                                                                                                                                                                                                                        aaaaTAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCATATTATTCGCCGGAAGTGTCTTAAATAATAAGTAGAATTCTAACTAAAAGGCTTCGCTAAg         >  1:835646/1‑250 (MQ=255)                                                                                                                                                                                                                                         aaaTAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCATATTATTCGCCGGAAGTGTCTTAAATAATAAGTAGAAt                                >  1:788255/1‑226 (MQ=255)                                                                                                                                                                                                                                         aaaTAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCATATTATTCGCCGGAAGTGTCTTAAATAATAAGTAGAATTCTAACTAAAAGGCTTCGCTAAGcc       <  1:449686/251‑1 (MQ=255)                                                                                                                                                                                                                                         aaaTAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCATATTATTCGCCGGAAGTGTCTTAAATAATAAGTAGAATTCTAACTAAAAGGCTTCGCTAAGcc       <  1:547760/251‑1 (MQ=255)                                                                                                                                                                                                                                              atCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCATATTATTCGCCGGAAGTGTCTTAaat                                            <  1:530129/209‑1 (MQ=255)                                                                                                                                                                                                                                              atCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCATATTATTCGCCGGAAGTGTCTTAAATAATAAGTAGAATTCTAACTAAAAGGCTTCGCTAAGCCAGCgg  >  1:816334/1‑251 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                   TATTTCTGAGTAAGTGCGGAAAAGAATTTACACCAAGTGCGGTCGTCTTACAAAACGTTTTTCATTATCAGAAAAGTACCAACCTGTTTGCAGATTCGCGCTTTAATGGAACAAATCTAAATCACATTTTTAGCATTTGAAGGGAGTGGAAAGACTGAAGAAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACTTTATTCATCGTGCCGCAACCGAAAATAATCTGATATTGTCCTGCTACGGCAAACTGCACTTTTACACCCTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTTGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTTCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGGCGATTTTAAGAAGATTCATATTATTCGCCGGAAGTGTCTTAAATAATAAGTAGAATTCTAACTAAAAGGCTTCGCTAAGCCAGCGG  >  1/1827865‑1828352

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑