Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,828,074	T→C	intergenic (+46174/+7310)	unknown → / ← unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,828,074	0	T	C	100.0%	195.2 / NA	65	intergenic (+46174/+7310)	unknown/unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (30/35);  total (30/35)

ACAAGGATTTTTTATTGTAATCCATGTAATACGCTTATTTCTGAGTAAGTGCGGAAAAGAATTTACACCAAGTGCGGTCGTCTTACAAAACGTTTTTCATTATCAGAAAAGTACCAACCTGTTTGCAGATTCGCGCTTTAATGGAACAAATCTAAATCACATTTTTAGCATTTGAAGGGAGTGGAAAGACTGAAGAAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACTTTATTCATCGTGCCGCAACCGAAAATAATCTGATATTGTCCTGCTACGGCAAACTGCACTTTTACACCCTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTTGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTTCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGGCGATTTTAAGAAGATTCATATTATTCGCCGGAAGTGTCTTAAATAATAAGT  >  1/1827830‑1828317                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                    aCAAGGATTTTTTATTGTAATCCATGTAATACGCTTATTTCTGAGTAAGTGCGGAAAAGAATTTACACCAAGTGCGGTCGTCTTACAAAACGTTTTTCATTATCAGAAAAGTACCAACCTGTTTGCAGATTCGCGCTTTAATGGAACAAATCTAAATCGCATTTTTAGCATTTGAAGGGAGTGGAAAGACTGAAGGAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTc                                                                                                                                                                                                                                               <  1:898126/251‑1 (MQ=255) aCAAGGATTTTTTATTGTAATCCATGTAATACGCTTATTTCTGAGTAAGTGCGGAAAAGAATTTACACCAAGTGCGGTCGTCTTACAAAACGTTTTTCATTATCAGAAAAGTACCAACCTGTTTGCAGATTCGCGCTTTAATGGAACAAATCTAAATCACATTTTTAGCATTTGAAGGGAGTGGAAAGACTGAAGGAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTc                                                                                                                                                                                                                                               <  1:761548/251‑1 (MQ=255)     ggATTTTTTATTGTAATCCATGTAATACGCTTATTTCTGAGTAAGTGCGGAAAAGAATTTACACCAAGTGCGGTCGTCTTACAAAACGTTTTTCATTATCAGAAAAGTACCAACCTGTTTGCAGATTCGCGCTTTAATGGAACAAATCTAAATCACATTTTTAGCATTTGAAGGGAGTGGAAAGACTGAAGGAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCg                                                                                                                                                                                                                                           >  1:146328/1‑251 (MQ=255)              aTTGTAATCCATGTAATACGCTTATTTCTGAGTAAGTGCGGAAAAGAATTTACACCAAGTGCGGTCGTCTTACAAAACGTTTTTCATTATCAGAAAAGTACCAACCTGTTTGCAGATTCGCGCTTTAATGGAACAAATCTAAATCACATTTTTAGCATTTGAAGGGAGTGGAAAGACTGAAGGAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCaa                                                                                                                                                                                                                                   >  1:840762/1‑250 (MQ=255)                   aaTCCATGTAATACGCTTATTTCTGAGTAAGTGCGGAAAAGAATTTACACCAAGTGCGGTCGTCTTACAAAACGTTTTTCATTATCAGAAAAGTACCAACCTGTTTGCAGATTCGCGCTTTAATGGAACAAATCTAAATCACATTTTTAGCATTTGAAGGGAGTGGAAAGACTGAAGGAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGaaa                                                                                                                                                                                                                             >  1:40342/1‑251 (MQ=255)                        aTGTAATACGCTTATTTCTGAGTAAGTGCGGAAAAGAATTTACACCAAGTGCGGTCGTCTTACAAAACGTTTTTCATTATCAGAAAAGTACCAACCTGTTTGCAGATTCGCGCTTTAATGGAACAAATCTAAATCACATTTTTAGCATTTGAAGGGAGTGGAAAGACTGAAGGAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAAc                                                                                                                                                                                                                                  <  1:336548/241‑1 (MQ=255)                          gTAATACGCTTATTTCTGAGTAAGTGCGGAAAAGAATTTACACCAAGTGCGGTCGTCTTACAAAACGTTTTTCATTATCAGAAAAGTACCAACCTGTTTGCAGATTCGCGCTTTAATGGAACAAATCTAAATCACATTTTTAGCATTTGAAGGGAGTGGAAAGACTGAAGGAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATc                                                                                                                                                                                                                       >  1:871654/1‑250 (MQ=255)                           tAATACGCTTATTTCTGAGTAAGTGCGGAAAAGAATTTACACCAAGTGCGGTCGTCTTACAAAACGTTTTTCATTATCAGAAAAGTACCAACCTGTTTGCAGATTCGCGCTTTAATGGAACAAATCTAAATCACATTTTTAGCATTTGAAGGGAGTGGAAAGACTGAAGGAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAaataat                                                                                                                                                                                                                        >  1:579300/1‑248 (MQ=255)                           tAATACGCTTATTTCTGAGTAAGTGCGGAAAAGAATTTACACCAAGTGCGGTCGTCTTACAAAACGTTTTTCATTATCAGAAAAGTACCAACCTGTTTGCAGATTCGCGCTTTAATGGAACAAATCTAAATCACATTTTTAGCATTTGAAGGGAGTGGAAAGACTGAAGGAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAaat                                                                                                                                                                                                                           <  1:382344/245‑1 (MQ=255)                               aCGCTTATTTCTGAGTAAGTGCGGAAAAGAATTTACACCAAGTGCGGTCGTCTTACAAAACGTTTTTCATTATCAGAAAAGTACCAACCTGTTTGCAGATTCGCGCTTTAATGGAACAAATCTAAATCACATTTTTAGCATTTGAAGGGAGTGGAAAGACTGAAGGAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGatac                                                                                                                                                                                                                 <  1:94502/251‑2 (MQ=255)                                 gCTTATTTCTGAGTAAGTGCGGAAAAGAATTTACACCAAGTGCGGTCGTCTTACAAAACGTTTTTCATTATCAGAAAAGTACCAACCTGTTTGCAGATTCGCGCTTTAATGGAACAAATCTAAATCACATTTTTAGCATTTGAAGGGAGTGGAAAGACTGAAGGAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGatac                                                                                                                                                                                                                 >  1:375802/1‑248 (MQ=255)                                 gCTTATTTCTGAGTAAGTGCGGAAAAGAATTTACACCAAGTGCGGTCGTCTTACAAAACGTTTTTCATTATCAGAAAAGTACCAACCTGTTTGCAGATTCGCGCTTTAATGGAACAAATCTAAATCACATTTTTAGCATTTGAAGGGAGTGGAAAGACTGAAGGAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGata                                                                                                                                                                                                                  >  1:263292/1‑248 (MQ=255)                                    tATTTCTGAGTAAGTGCGGAAAAGAATTTACACCAAGTGCGGTCGTCTTACAAAACGTTTTTCATTATCAGAAAAGTACCAACCTGTTTGCAGATTCGCGCTTTAATGGAACAAATCTAAATCACATTTTTAGCATTTGAAGGGAGTGGAAAGACTGAAGGAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGGCCGTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTcc                                                                                                                                                                                                            <  1:242860/251‑1 (MQ=255)                                         tctaagtaatggcgAAAAAAAATTACACCAAGTGCGGTCGTCTTACAAAACGTTTTTCATTATCAGAAAAGTACCAACCTGTTTGCAGATTCGCGCTTTAATGGAACAAATCTAAATCACATTTTTAGCATTTGAAGGGAGTGGAAAGACTGAAGGAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTa                                                                                                                                                                                                       <  1:307425/238‑1 (MQ=255)                                                        aaaGAATTTACACCAAGTGCGGTCGTCTTACAAAACGTTTTTCATTATCAGAAAAGTACCAACCTGTTTGCAGATTCGCGCTTTAATGGAACAAATCTAAATCACATTTTTAGCATTTGAAGGGAGTGGAAAGACTGAAGGAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTc                                                                                                                                                                                                             >  1:90303/1‑230 (MQ=255)                                                            aaTTTACACCAAGTGCGGTCGTCTTACAAAACGTTTTTCATTATCAGAAAAGTACCAACCTGTTTGCAGATTCGCGCTTTAATGGAACAAATCTAAATCACATTTTTAGCATTTGAAGGGAGTGGAAAGACTGAAGGAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTa                                                                                                                                                                                     >  1:637919/1‑250 (MQ=255)                                                                      aaGTGCGGTCGTCTTACAAAACGTTTTTCATTATCAGAAAGGTGCCAACCTGTTTGCAGATTCGCGCTTTAATGGAACAAATCTAAATCACATTTTTAGCATTTGAAGGGAGTGGAAAGACTGAAGGAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAAtg                                                                                                                                                                          <  1:346536/251‑1 (MQ=255)                                                                      aaGTGCGGTCGTCTTACAAAACGTTTTTCATTATCAGAAAAGTACCAACCTGTTTGCAGATTCGCGCTTTAATGGAACAAATCTAAATCACATTTTTAGCATTTGAAGGGAGTGGAAAGACTGAAGGAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAAtg                                                                                                                                                                          >  1:796887/1‑251 (MQ=255)                                                                      aaGTGCGGTCGTCTTACAAAACGTTTTTCATTATCAGAAAAGTACCAACCTGTTTGCAGATTCGCGCTTTAATGGAACAAATCTAAATCACATTTTTAGCATTTGAAGGGAGTGGAAAGACTGAAGGAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAAtg                                                                                                                                                                          <  1:656002/251‑1 (MQ=255)                                                                      aaGTGCGGTCGTCTTACAAAACGTTTTTCATTATCAGAAAAGTACCAACCTGTTTGCAGATTCGCGCTTTAATGGAACAAATCTAAATCACATTTTTAGCATTTGAAGGGAGTGGAAAGACTGAAGGAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAAtg                                                                                                                                                                          <  1:182422/251‑1 (MQ=255)                                                                             gtcgtcTTACAAAACGTTTTTCATTATCAGAAAAGTACCAACCTGTTTGCAGATTCGCGCTTTAATGGAACAAATCTAAATCACATTTTTAGCATTTGAAGGGAGTGGAAAGACTGAAGGAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCa                                                                                                                                                                    <  1:802281/250‑1 (MQ=255)                                                                                gtcTTACAAAACGTTTTTCATTATCAGAAAAGTACCAACCTGTTTGCAGATTCGCGCTTTAATGGAACAAATCTAAATCACATTTTTAGCATTTGAAGGGAGTGGAAAGACTGAAGGAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTc                                                                                                                                                                                                                                               <  1:135962/172‑1 (MQ=255)                                                                                         aaCGTTTTTCATTATCAGAAAAGTACCAACCTGTTTGCAGATTCGCGCTTTAATGGAACAAATCTAAATCACATTTTTAGCATTTGAAGGGAGTGGAAAGACTGAAGGAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAAc                                                                                                                                                       <  1:778064/251‑1 (MQ=255)                                                                                          aCGTTTTTCATTATCAGAAAAGTACCAACCTGTTTGCAGATTCGCGCTTTAATGGAACAAATCTAAATCACATTTTTAGCATTTGAAGGGAGTGGAAAGACTGAAGGAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGatac                                                                                                                                                                                                                 <  1:135228/192‑2 (MQ=255)                                                                                            gTTTTTCATTATCAGAAAAGTACCAACCTGTTTGCAGATTCGCGCTTTAATGGAACAAATCTAAATCACATTTTTAGCATTTGAAGGGAGTGGAAAGACTGAAGGAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACttt                                                                                                                                                    >  1:451681/1‑251 (MQ=255)                                                                                                      aTCAGAAAAGTACCAACCTGTTTGCAGATTCGCGCTTTAATGGAACAAATCTAAATCACATTTTTAGCATTTGAAGGGAGTGGAAAGACTGAAGGAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTac                                                                                                                                                                                    >  1:444507/1‑209 (MQ=255)                                                                                                              aGTACCAACCTGTTTGCAGATTCGCGCTTTAATGGAACAAATCTAAATCACATTTTTAGCATTTGAAGGGAGTGGAAAGACTGAAGGAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGaa                                                                                                                                   >  1:297441/1‑250 (MQ=255)                                                                                                                tgccAACCTGTTTGCAGATTCGCGCTTTAATGGAACAAATCTAAATCACATTTTTAGCATTTGAAGGGAGTGGAAAGACTGAAGGAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGaacaa                                                                                                                                <  1:320466/249‑1 (MQ=255)                                                                                                                      ccTGTTTGCAGATTCGCGCTTTAATGGAACAAATCTAAATCACATTTTTAGCATTTGAAGGGAGTGGAAAGACTGAAGGAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACgcgc                                                                                                                           >  1:239768/1‑250 (MQ=255)                                                                                                                              cAGATTCGCGCTTTAATGGAACAAATCTAAATCACATTTTTAGCATTTGAAGGGAGTGGAAAGACTGAAGGAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCAt                                                                                                                   <  1:130361/250‑1 (MQ=255)                                                                                                                                gATTCGCGCTTTAATGGAACAAATCTAAATCACATTTTTAGCATTTGAAGGGAGTGGAAAGACTGAAGGAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATgg                                                                                                                 >  1:132615/1‑250 (MQ=255)                                                                                                                                 aTTCGCGCTTTAATGGAACAAATCTAAATCACATTTTTAGCATTTGAAGGGAGTGGAAAGACTGAAGGAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGgc                                                                                                                <  1:399774/250‑1 (MQ=255)                                                                                                                                        cTTTAATGGAACAAATCTAAATCACATTTTTAGCATTTGAAGGGAGTGGAAAGACTGAAGGAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTggg                                                                                                        >  1:740367/1‑251 (MQ=255)                                                                                                                                        cTTTAATGGAACAAATCTAAATCACATTTTTAGCATTTGAAGGGAGTGGAAAGACTGAAGGAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTggg                                                                                                        >  1:629246/1‑251 (MQ=255)                                                                                                                                        cTTTAATGGAACAAATCTAAATCACATTTTTAGCATTTGAAGGGAGCGGAAAGACTGAAGGAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTggg                                                                                                        >  1:441028/1‑251 (MQ=255)                                                                                                                                          ttAATGGAACAAATCTAAATCACATTTTTAGCATTTGAAGGGAGTGGAAAGACTGAAGGAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTa                                                                                                      >  1:802919/1‑251 (MQ=255)                                                                                                                                           tAATGGAACAAATCTAAATCACATTTTTAGCATTTGAAGGGAGTGGAAAGACTGAAGGAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGt                                                                                                       <  1:676829/249‑1 (MQ=255)                                                                                                                                                gAACAAATCTAAATCACATTTTTAGCATTTGAAGGGAGTGGAAAGACTGAAGGAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCtt                                                                                                                                                >  1:651220/1‑203 (MQ=255)                                                                                                                                                gAACAAATCTAAATCACATTTTTAGCATTTGAAGGGAGTGGAAAGACTGAAGGAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGaac                                                                                                                                  >  1:215402/1‑217 (MQ=255)                                                                                                                                                     aaTCTAAATCACATTTTTAGCATTTGAAGGGAGTGGAAAGACTGAAGGAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTa                                                                                                                                                                                                       <  1:43997/143‑1 (MQ=255)                                                                                                                                                       tCTAAATCACATTTTTAGCATTTGAAGGGAGTGGAAAGACTGAAGGAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAatat                                                                                         <  1:50502/251‑1 (MQ=255)                                                                                                                                                                         cATTTGAAGGGAGTGGAAAGACTGAAGGAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTc                                                                                                                                                                                                                                               >  1:345458/1‑83 (MQ=255)                                                                                                                                                                           tttGAAGGGAGTGGAAAGACTGAAGGAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACttt                                                                                                                                                    <  1:32886/172‑1 (MQ=255)                                                                                                                                                                           tttGAAGGGAGTGGAAAGACTGAAGGAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGgcgc                                                                                                              <  1:834626/210‑1 (MQ=255)                                                                                                                                                                           tttGAAGGGAGTGGAAAGACTGAAGGAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGtttt                                                                     >  1:702419/1‑251 (MQ=255)                                                                                                                                                                                 gggAGTGGAAAGACTGAAGGAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTg                                                                                                  >  1:191264/1‑216 (MQ=255)                                                                                                                                                                                  ggAGTGGAAAGACTGAAGGAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATaa                                                              >  1:677390/1‑251 (MQ=255)                                                                                                                                                                                   gAGTGGAAAGACTGAAGGAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGatac                                                                                                                                                                                                                 <  1:116176/103‑2 (MQ=255)                                                                                                                                                                                      tGGAAAGACTGAAGGAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGgc                                                                                                                <  1:95511/197‑1 (MQ=255)                                                                                                                                                                                       ggAAAGACAGAAGGAAATAAAGAAAGCAGCATACCGGGCTATTTAATCAATTCGGCGTGGACCTTATTCATCGTTACGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACCCCTTCAAtg                                                                                                                                                                          <  1:768614/138‑1 (MQ=255)                                                                                                                                                                                           aGACTGAAGGAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATAtttcct                                                                                    >  1:26748/1‑217 (MQ=255)                                                                                                                                                                                           aGACTGAAGGAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATAtttc                                                                                      >  1:627086/1‑217 (MQ=255)                                                                                                                                                                                                 aaggaaaTAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGAttt                                               <  1:151209/247‑1 (MQ=255)                                                                                                                                                                                                      aaTAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTaaga                                          <  1:142420/251‑1 (MQ=255)                                                                                                                                                                                                      aaTAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTaaga                                          <  1:383584/251‑1 (MQ=255)                                                                                                                                                                                                         aaagaaagCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATAtttcct                                                                                    >  1:735056/1‑203 (MQ=255)                                                                                                                                                                                                                gcagcaTACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACGCCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGa                                                  <  1:791399/233‑1 (MQ=255)                                                                                                                                                                                                                    caTACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCAtatt                               <  1:695531/248‑1 (MQ=255)                                                                                                                                                                                                                      tACCGGGCCATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCATATTATTCg                          >  1:149741/1‑251 (MQ=255)                                                                                                                                                                                                                             ccATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCATATTATTCGCCGGAAg                   <  1:869287/251‑1 (MQ=255)                                                                                                                                                                                                                             ccATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCATATTATTCGCCGGAAg                   <  1:303686/251‑1 (MQ=255)                                                                                                                                                                                                                              cATTTAATCAATTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCATATTATTCGCCGGAAgt                  >  1:67364/1‑251 (MQ=255)                                                                                                                                                                                                                                       aaTTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCATATTATTCGCCGGAAGTGTCTTAaat         <  1:237041/251‑1 (MQ=255)                                                                                                                                                                                                                                       aaTTCGGCGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCATATTATTCGCCGGAAGTGTCTTAaat         <  1:795451/251‑1 (MQ=255)                                                                                                                                                                                                                                              cGTGGACCTTATTCATCGTTCCGCAACCGAAAATAATCTGATACTGTCCTGCTACGGCAAACTGCACTTTTACACCTTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTCGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTCCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGACGATTTTAAGAAGATTCATATTATTCGCCGGAAGTGTCTTAAATAATAAGt  <  1:710246/251‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                    ACAAGGATTTTTTATTGTAATCCATGTAATACGCTTATTTCTGAGTAAGTGCGGAAAAGAATTTACACCAAGTGCGGTCGTCTTACAAAACGTTTTTCATTATCAGAAAAGTACCAACCTGTTTGCAGATTCGCGCTTTAATGGAACAAATCTAAATCACATTTTTAGCATTTGAAGGGAGTGGAAAGACTGAAGAAAATAAAGAAAGCAGCATACCGGGCCATTTAATCAATTCGGCGTGGACTTTATTCATCGTGCCGCAACCGAAAATAATCTGATATTGTCCTGCTACGGCAAACTGCACTTTTACACCCTCAATGTTGTCAATGCCTTGTCAACTTTGCTTTTGTTATTCAGAACAACGCGCCAACGCATGGCGCAGTGGGTAGCTGTCACAATATTTTCTTTGTCGCCAAGTTTTTCAATAACCTGTTGGGCGATTTTAAGAAGATTCATATTATTCGCCGGAAGTGTCTTAAATAATAAGT  >  1/1827830‑1828317

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑