Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,821,807	T→A	intergenic (+39907/+13577)	unknown → / ← unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,821,807	0	T	A	100.0%	131.2 / NA	48	intergenic (+39907/+13577)	unknown/unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (23/25);  total (23/25)

ACACATCTTTGAGATTGATATTTGCTAATGATAATTTATCTTTATCACCGCTATGTTCCGTAATAGTGTGGTGCCCATATTCTTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGGTCATCGCCTTCTCCACCGTCAAGTAAATCATCTCCTTGGTTTCCAAACAACTTATCATTGCCACTTCCACCTCTTAGAATATCGGAACCATTGCCTCCCTCTAAGTGGTCGTCGCCTTCTCCACCGTCAAGATAGTTGTTGCCATATTCACCAAAGAGCCTATCGTTACCGGCACCACCATAGAGCTTATCATTACCTTGGCCGCCATGAATTTCGTCATTCCCATTATCGCCATACAAACGATCATCGCCATCATAACCATAAATCAAATCATCGCCATCGTGACCATGGAAAACATCATTAAATTTAGAACCATAAAATTTATCACGTAGTGTGCTGCCGATAATTTCCTCTACTGAGTTTA  >  1/1821560‑1822051                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                     acacCTCTTTGAGGTTGATATTTGCTAATGATAATTTATCTTTATCACCGCTATGTTCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCGAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCa                                                                                                                                                                                                                                                   <  1:19770/251‑1 (MQ=255)       cTTTGAGGTTGATATTTGCTAATGATAATTTATCTTTATCACCGCTATGTTCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGt                                                                                                                                                                                                                                               >  1:676970/1‑249 (MQ=255)       cTTTGAGGTTGATATTTGCTAATGATAATTTATCTTTATCACCGCTATGTTCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGt                                                                                                                                                                                                                                               >  1:24648/1‑249 (MQ=255)         ttGAGGTTGATATTTGCTAATGATAATTTATCTTTATCACCGCTATGTTCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTctag                                                                                                                                                                                                                                           <  1:92271/251‑4 (MQ=255)            aggttGATATTTGCTAATGATAATTTATCTTTATCACCGCTATGTTCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTTCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGAt                                                                                                                                                                                                                                         <  1:178795/247‑1 (MQ=255)            aggttGATATTTGCTAATGATAATTTATCTTTATCACCGCTATGTTCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGAt                                                                                                                                                                                                                                         <  1:461324/247‑1 (MQ=255)            aggttGATATTTGCTAATGATAATTTATCTTTATCACCGCTATGTTCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGAt                                                                                                                                                                                                                                         <  1:793025/247‑1 (MQ=255)            aggttGATATTTGCTAATGATAATTTATCTTTATCACCGCTATGTTCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGAt                                                                                                                                                                                                                                         <  1:604227/247‑1 (MQ=255)              gttGATATTTGCTAATGATAATTTATCTTTATCACCGCTATGTTCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGAt                                                                                                                                                                                                                                         >  1:672489/2‑248 (MQ=255)                tGATATTTGCTAATGATAATTTATCTTTATCACCGCTATGTTCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAgttg                                                                                                                                                                                                                                    <  1:236144/251‑1 (MQ=255)                         cTAATGATAATTTATCTTTATCACCGCTATGTTCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCatat                                                                                                                                                                                                                           <  1:600827/251‑1 (MQ=255)                                tAATTTATCTTTATCACCGCTATGTTCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCaa                                                                                                                                                                                                                    <  1:660471/251‑1 (MQ=255)                                  aTTTATCTTTATCACCGCTATGTTCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCAAag                                                                                                                                                                                                                  <  1:549865/251‑1 (MQ=255)                                                ccGCTATGTTCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTcaccac                                                                                                                                                                                                                                                  >  1:895642/1‑205 (MQ=255)                                                ccGCTATGTTCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCAAAGAGCCtgtcattgc                                                                                                                                                                                                     >  1:385254/1‑242 (MQ=255)                                                     aTGTTCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAgttg                                                                                                                                                                                                                                    <  1:419764/214‑1 (MQ=255)                                                       gTTCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCAAAGAGCCTGTCATTGCCGGcgcca                                                                                                                                                                                             >  1:527365/1‑251 (MQ=255)                                                        ttCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTcaccac                                                                                                                                                                                                                                                  >  1:654430/1‑197 (MQ=255)                                                          ccGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCat                                                                                                                                                                                                                             >  1:843668/1‑216 (MQ=255)                                                          ccGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCAAAGAGCCTGTCATTGCCGGCGccacca                                                                                                                                                                                          >  1:154513/1‑251 (MQ=255)                                                          ccGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCAAAGAGCCTGTCATTGCCGGCGccacca                                                                                                                                                                                          >  1:540493/1‑251 (MQ=255)                                                          ccGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCAAAGAGCCTGTCATTGCCGGCGccacca                                                                                                                                                                                          >  1:667289/1‑251 (MQ=255)                                                               aTAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCAAagag                                                                                                                                                                                                                <  1:113284/224‑1 (MQ=255)                                                               aTAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCAAAGAGCCtgtcattg                                                                                                                                                                                                      <  1:439535/234‑8 (MQ=255)                                                               aTAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCAAAGAGCCTGTCATTGCCGGCGCCACCATaaag                                                                                                                                                                                     <  1:86129/251‑4 (MQ=255)                                                               aTAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCAAAGAGCCTGTCATTGCCGGCGCCACCATaaag                                                                                                                                                                                     >  1:319844/1‑248 (MQ=255)                                                                tAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCAAAGAGCCTGTCATTGCCGGCGCCACCATaaagt                                                                                                                                                                                    <  1:482596/251‑5 (MQ=255)                                                                 aGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCAAAGAGCCTGTCATTGCCGGCGCCACCATaaagtt                                                                                                                                                                                   >  1:289420/1‑246 (MQ=255)                                                                  gtgtGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCAAAGAGCCTGTCATTGCCGGCGCCACCATaaagttt                                                                                                                                                                                  >  1:79381/1‑245 (MQ=255)                                                                    gtggtgGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCata                                                                                                                                                                                                                            >  1:163873/1‑207 (MQ=255)                                                                      ggtgGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCAAAGAGCCTGTCATTGCCGGCGCCACCATaaagtttgtcg                                                                                                                                                                              >  1:611300/1‑241 (MQ=255)                                                                         ggccATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCAAAGAGCCTGTCATTGCCGGCGCCACCATaaagtttgtcgttg                                                                                                                                                                           >  1:340776/3‑238 (MQ=255)                                                                          gccATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGAt                                                                                                                                                                                                                                         <  1:135531/187‑1 (MQ=255)                                                                          gccATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCAAAGAGCCTGTCATTGCCGGCGCCACCATaaagtttgtcgttg                                                                                                                                                                           >  1:596773/2‑237 (MQ=255)                                                                          gccATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCAAAGAGCCTGTCATTGCCGGCGCCACCATaaagtttgtcgttg                                                                                                                                                                           >  1:226947/2‑237 (MQ=255)                                                                               atTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCTTTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCaa                                                                                                                                                                                                                    >  1:760071/1‑204 (MQ=255)                                                                                             aCATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCAAAGAGCCtgtcattgcc                                                                                                                                                                                                    >  1:669250/1‑197 (MQ=255)                                                                                                                                                                                       tCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCAAAGAGCCTGTCATTGCCGGCGCCACCATAAAGTTTGTCGTTGCCTTTTCCTCCATGGATCTCATCATTACCATTATCTCCATATAAGCGGTCATCACCATCATAACCATAAATCACATCATCACCATCGTGACCATGGAAAACATCATTaaa                                                             <  1:870761/251‑1 (MQ=255)                                                                                                                                                                                                       ctctTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCAAAGAGCCTGTCATTGCCGGCGCCACCATAAAGTTTGTCGTTGCCTTTTCCTCCATGGATCTCATCATTACCATTATCTCCATATAAGCGGTCATCACCATCATAACCATAAATCACATCATCACCATCGTGACCATGGAAAACATCATTAAATCTAGAACCATaaaaa                                             <  1:885681/251‑2 (MQ=255)                                                                                                                                                                                                        tctTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCAAAGAGCCTGTCATTGCCGGCGCCACCATAAAGTTTGTCGTTGCCTTTTCCTCCATGGATCTCATCATTACCATTATCTCCATATAAGCGGTCATCACCATCATAACCATAAATCACATCATCACCATCGTGACCATGGAAAACATCATTAAAt                                                            <  1:471351/235‑1 (MQ=255)                                                                                                                                                                                                        tctTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCAAAGAGCCTGTCATTGCCGGCGCCACCATAAAGTTTGTCGTTGCCTTTTCCTCCATGGATCTCATCATTACCATTATCTCCATATAAGCGGTCATCACCATCATAACCATAAATCACATCATCACCATCGTGACCATGGAAAACATCATTAAATCTAGAACCATaaaaat                                            >  1:273575/1‑249 (MQ=255)                                                                                                                                                                                                         ctTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCAAAGAGCCTGTCATTGCCGGCGCCGCCATAAAGTTTGTCGTTGCCTTTTCCTCCATGGATCTCATCATTACCATTATCTCCATATAAGCGGTCATCACCATCATAACCATAAATCACATCATCACCATCGTGACCATGGAAAACATCATTAAATCTAGAACCATaaaaatg                                           <  1:210238/251‑4 (MQ=255)                                                                                                                                                                                                                       aCCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCAAAGAGCCTGTCATTGCCGGCGCCACCATAAAGTTTGTCGTTGCCTTTTCCTCCATGGATCTCATCATTACCATTATCTCCATATAAGCGGTCATCACCATCATAACCATAAATCACATCATCACCATCGTGACCATGGAAAACATCATTAAATCTAGAACCATaaaaatgatcccgtaatttac                             <  1:693251/251‑18 (MQ=255)                                                                                                                                                                                                                       aCCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCAAAGAGCCTGTCATTGCCGGCGCCACCATAAAGTTTGTCGTTGCCTTTTCCTCCATGGATCTCATCATTACCATTATCTCCATATAAGCGGTCATCACCATCATAACCATAAATCACATCATCACCATCGTGACCATGGAAAACATCATTAAATCTAGAACCATaaaaatgatcccgtaatttac                             <  1:344235/251‑18 (MQ=255)                                                                                                                                                                                                                             ccccccttctAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCAAAGAGCCTGTCATTGCCGGCGCCACCATAAAGTTTGTCGTTGCCTTTTCCTCCATGGATCTCATCATTACCATTATCTCCATATAAGCGGTCATCACCATCATAACCATAAATCACATCATCACCATCGTGACCATGGAAAACATCATTAAATCTAGAACCATaa                                                >  1:500519/8‑226 (MQ=255)                                                                                                                                                                                                                                            gtcgtcGCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCAAAGAGCCTGTCATTGCCGGCGCCACCATAAAGTTTGTCGTTGCCTTTTCCTCCATGGATCTCATCATTACCATTATCTCCATATAAGCGGTCATCACCATCATAACCATAAATCACATCATCACCATCGTGACCATGGAAAACATCATTAAATCTAGAACCATAAAAATGATCCCGTAATTTACTGCCGATAATTTCCTCTAc          <  1:420430/249‑1 (MQ=255)                                                                                                                                                                                                                                                tcgCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCAAAGAGCCTGTCATTGCCGGCGCCACCATAAAGTTTGTCGTTGCCTTTTCCTCCATGGATCTCATCATTACCATTATCTCCATATAAGCGGTCATCACCATCATAACCATAAATCACATCATCACCATCGTGACCATGGAAAACATCATTAAATCTAGAACCATAAAAATGATCCCGTAATTTACTGCCGATAATTTCCTCTACTGAGt     <  1:191139/250‑1 (MQ=255)                                                                                                                                                                                                                                                  gCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCAAAGAGCCTGTCATTGCCGGCGCCACCATAAAGTTTGTCGTTGCCTTTTCCTCCATGGATCTCATCATTACCATTATCTCCATATAAGCGGTCATCACCATCATAACCATAAATCACATCATCACCATCGTGACCATGGAAAACATCATTAAATCTAGAACCATAAAAATGATCCCGTAATTTACTGCCGATAATTTCCTCTACTGAGTTTa  >  1:570569/1‑251 (MQ=255)                                                                                                                                                                                                                                                   ccTTCACCACCGTCTAGATAGTTGTTACCATATTCACCAAAGAGCCTGTCATTGCCGGCGCCACCATAAAGTTCGTCGTTGCCTTTTCCTCCATGGATCTCATCATTACCATTATCTCCATATAAGCGGTCATCACCATCATAACCATAAATCACATCATCACCATCGTGACCATGGAAAACATCATTAAATCTAGAACCATAAAAATGATCCCGTAATTTACTGCCGATAATTTCCTCTAc          <  1:751858/242‑1 (MQ=255)                                                                                                                                                                                                                                                    cTTCACCACCGTCTAGATAGTTGTTACCATATTCACCAAAGAGCCTGTCATTGCCGGCGCCACCATAAAGTTTGTCGTTGCCTTTTCCTCCATGGATCTCATCATTACCATTATCTCCATATAAGCGGTCATCACCATCATAACCATAAATCACATCATCACCATCGTGACCATGGAAAACATCATTAAAtc                                                           >  1:300832/1‑191 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                     ACACATCTTTGAGATTGATATTTGCTAATGATAATTTATCTTTATCACCGCTATGTTCCGTAATAGTGTGGTGCCCATATTCTTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGGTCATCGCCTTCTCCACCGTCAAGTAAATCATCTCCTTGGTTTCCAAACAACTTATCATTGCCACTTCCACCTCTTAGAATATCGGAACCATTGCCTCCCTCTAAGTGGTCGTCGCCTTCTCCACCGTCAAGATAGTTGTTGCCATATTCACCAAAGAGCCTATCGTTACCGGCACCACCATAGAGCTTATCATTACCTTGGCCGCCATGAATTTCGTCATTCCCATTATCGCCATACAAACGATCATCGCCATCATAACCATAAATCAAATCATCGCCATCGTGACCATGGAAAACATCATTAAATTTAGAACCATAAAATTTATCACGTAGTGTGCTGCCGATAATTTCCTCTACTGAGTTTA  >  1/1821560‑1822051

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑