Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,821,735	C→T	intergenic (+39835/+13649)	unknown → / ← unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,821,735	0	C	T	100.0%	172.3 / NA	60	intergenic (+39835/+13649)	unknown/unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (32/28);  total (32/28)

TGTTACTGCTGTTCTATTATTTGTTTTCAATAGTAGATCATTGCCGTTACGCTCAAATGACACATCTTTGAGATTGATATTTGCTAATGATAATTTATCTTTATCACCGCTATGTTCCGTAATAGTGTGGTGCCCATATTCTTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGGTCATCGCCTTCTCCACCGTCAAGTAAATCATCTCCTTGGTTTCCAAACAACTTATCATTGCCACTTCCACCTCTTAGAATATCGGAACCATTGCCTCCCTCTAAGTGGTCGTCGCCTTCTCCACCGTCAAGATAGTTGTTGCCATATTCACCAAAGAGCCTATCGTTACCGGCACCACCATAGAGCTTATCATTA  >  1/1821501‑1821882                                                                                                                                                                                                                                           |                                                                                                                                                    tGTTACTGCTGTTCTATTATTTGTTTTCAATAGTAGATCATTGCCGTTACGTTCAAATGACACCTCTTTGAGGTTGATATTTGCTAATGATAATTTATCTTTATCACCGCTATGTTCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCa                                                                                                                                      >  1:880111/1‑250 (MQ=255)    tACTGCTGTTCTATTATTTGTTTTCAATAGTAGATCATTGCCGTTACGTTCAAATGACACCTCTTTGAGGTTGATATTTGCTAATGATAATTTATCTTTATCACCGCTATGTTCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTc                                                                                                                                  <  1:573313/251‑1 (MQ=255)           gTTCTATTATTTGTTTTCAATAGTAGATCATTGCCGTTACGTTCAAATGACACCTCTTTGAGGTTGATATTTGCTAATGATAATTTATCTTTATCACCGCTATGTTCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACtt                                                                                                                                   <  1:542347/243‑1 (MQ=255)           gTTCTATTATTTGTTTTCAATAGTAGATCATTGCCGTTACGTTCAAATGACACCTCTTTGAGGTTGATATTTGCTAATGATAATTTATCTTTATCACCGCTATGTTCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCct                                                                                                                             >  1:75758/1‑249 (MQ=255)           gTTCTATTATTTGTTTTCAATAGTAGATCATTGCCGTTACGTTCAAATGACACCTCTTTGAGGTTGATATTTGCTAATGATAATTTATCTTTATCACCGCTATGTTCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCct                                                                                                                             >  1:438786/1‑249 (MQ=255)                 ttattTGTTTTCAATAGTAGATCATTGCCGTTACGTTCAAATGACACCTCTTTGAGGTTGATATTTGCTAATGATAATTTATCTTTATCACCGCTATGTTCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAat                                                                                                                     <  1:310299/251‑1 (MQ=255)                                 gtagATCATTGCCGTTACGTTCAAATGACACCTCTTTGAGGTTGATATTTGCTAATGATAATTTATCTTTATCACCGCTATGTTCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCAttccc                                                                                                      >  1:175079/1‑247 (MQ=255)                                 gtagATCATTGCCGTTACGTTCAAATGACACCTCTTTGAGGTTGATATTTGCTAATGATAATTTATCTTTATCACCGCTATGTTCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCAttccc                                                                                                      >  1:383459/1‑247 (MQ=255)                                  tagATCATTGCCGTTACGTTCAAATGACACCTCTTTGAGGTTGATATTTGCTAATGATAATTTATCTTTATCACCGCTATGTTCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCAttccccc                                                                                                    <  1:78416/251‑6 (MQ=255)                                            ccGTTACGTTCAAATGACACCTCTTTGAGGTTGATATTTGCTAATGATAATTTATCTTTATCACCGCTATGTTCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTaaat                                                                                           <  1:510923/250‑3 (MQ=255)                                                     tCAAATGACACCTCTTTGAGGTTGATATTTGCTAATGATAATTTATCTTTATCACCGCTATGTTCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGcc                                                                                 <  1:825617/251‑1 (MQ=255)                                                            acacCTCTTTGAGGTTGATATTTGCTAATGATAATTTATCTTTATCACCGCTATGTTCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCGAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCa                                                                          <  1:19770/251‑1 (MQ=255)                                                            acacCTCTTTGAGGTTGATATTTGCTAATGATAATTTATCTTTATCACCGCTATGTTCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTtcacc                                                                           <  1:47213/250‑4 (MQ=255)                                                                ctCTTTGAGGTTGATATTTGCTAATGATAATTTATCTTTATCACCGCTATGTTCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTtcacc                                                                           <  1:413163/245‑4 (MQ=255)                                                                  cTTTGAGGTTGATATTTGCTAATGATAATTTATCTTTATCACCGCTATGTTCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGt                                                                      >  1:24648/1‑249 (MQ=255)                                                                  cTTTGAGGTTGATATTTGCTAATGATAATTTATCTTTATCACCGCTATGTTCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGt                                                                      >  1:676970/1‑249 (MQ=255)                                                                    ttGAGGTTGATATTTGCTAATGATAATTTATCTTTATCACCGCTATGTTCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTctag                                                                  <  1:92271/251‑4 (MQ=255)                                                                       aggttGATATTTGCTAATGATAATTTATCTTTATCACCGCTATGTTCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTTCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGAt                                                                <  1:178795/247‑1 (MQ=255)                                                                       aggttGATATTTGCTAATGATAATTTATCTTTATCACCGCTATGTTCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGAt                                                                <  1:793025/247‑1 (MQ=255)                                                                       aggttGATATTTGCTAATGATAATTTATCTTTATCACCGCTATGTTCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGAt                                                                <  1:604227/247‑1 (MQ=255)                                                                       aggttGATATTTGCTAATGATAATTTATCTTTATCACCGCTATGTTCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGAt                                                                <  1:461324/247‑1 (MQ=255)                                                                         gttGATATTTGCTAATGATAATTTATCTTTATCACCGCTATGTTCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGAt                                                                >  1:672489/2‑248 (MQ=255)                                                                           tGATATTTGCTAATGATAATTTATCTTTATCACCGCTATGTTCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAgttg                                                           <  1:236144/251‑1 (MQ=255)                                                                                    cTAATGATAATTTATCTTTATCACCGCTATGTTCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCatat                                                  <  1:600827/251‑1 (MQ=255)                                                                                           tAATTTATCTTTATCACCGCTATGTTCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCaa                                           <  1:660471/251‑1 (MQ=255)                                                                                             aTTTATCTTTATCACCGCTATGTTCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCAAag                                         <  1:549865/251‑1 (MQ=255)                                                                                                        tcACCGCTATGTTCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATgg                                                                                         <  1:395947/192‑1 (MQ=255)                                                                                                           ccGCTATGTTCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTaaat                                                                                           <  1:29080/187‑3 (MQ=255)                                                                                                           ccGCTATGTTCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTcaccac                                                                         >  1:895642/1‑205 (MQ=255)                                                                                                           ccGCTATGTTCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCAAAGAGCCtgtcattgc                            >  1:385254/1‑242 (MQ=255)                                                                                                                aTGTTCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAgttg                                                           <  1:419764/214‑1 (MQ=255)                                                                                                                  gTTCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCAttcccc                                                                                                     >  1:840479/1‑166 (MQ=255)                                                                                                                  gTTCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCAAAGAGCCTGTCATTGCCGGcgcca                    >  1:527365/1‑251 (MQ=255)                                                                                                                   ttCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTcaccac                                                                         >  1:654430/1‑197 (MQ=255)                                                                                                                     ccGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCat                                                    >  1:843668/1‑216 (MQ=255)                                                                                                                     ccGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCAAAGAGCCTGTCATTGCCGGCGccacca                 >  1:667289/1‑251 (MQ=255)                                                                                                                     ccGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCAAAGAGCCTGTCATTGCCGGCGccacca                 >  1:540493/1‑251 (MQ=255)                                                                                                                     ccGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCAAAGAGCCTGTCATTGCCGGCGccacca                 >  1:154513/1‑251 (MQ=255)                                                                                                                       gTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCAttccccccttc                                                                                                >  1:240867/1‑161 (MQ=255)                                                                                                                       gTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCAttcccccc                                                                                                   <  1:355894/167‑7 (MQ=255)                                                                                                                       gTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTaaa                                                                                            >  1:246988/1‑173 (MQ=255)                                                                                                                          aTAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCAAagag                                       <  1:113284/224‑1 (MQ=255)                                                                                                                          aTAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCAAAGAGCCtgtcattg                             <  1:439535/234‑8 (MQ=255)                                                                                                                          aTAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCAAAGAGCCTGTCATTGCCGGCGCCACCATaaag            <  1:86129/251‑4 (MQ=255)                                                                                                                          aTAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCAAAGAGCCTGTCATTGCCGGCGCCACCATaaag            >  1:319844/1‑248 (MQ=255)                                                                                                                           tAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCAAAGAGCCTGTCATTGCCGGCGCCACCATaaagt           <  1:482596/251‑5 (MQ=255)                                                                                                                            aGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCAAAGAGCCTGTCATTGCCGGCGCCACCATaaagtt          >  1:289420/1‑246 (MQ=255)                                                                                                                             gtgtGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCAAAGAGCCTGTCATTGCCGGCGCCACCATaaagttt         >  1:79381/1‑245 (MQ=255)                                                                                                                               gtggtgGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCata                                                   >  1:163873/1‑207 (MQ=255)                                                                                                                                 ggtgGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCAAAGAGCCTGTCATTGCCGGCGCCACCATaaagtttgtcg     >  1:611300/1‑241 (MQ=255)                                                                                                                                    ggccATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCAAAGAGCCTGTCATTGCCGGCGCCACCATaaagtttgtcgttg  >  1:340776/3‑238 (MQ=255)                                                                                                                                     gccATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAAc                                                                                                             >  1:787529/2‑143 (MQ=255)                                                                                                                                     gccATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGAt                                                                <  1:135531/187‑1 (MQ=255)                                                                                                                                     gccATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCAAAGAGCCTGTCATTGCCGGCGCCACCATaaagtttgtcgttg  >  1:596773/2‑237 (MQ=255)                                                                                                                                     gccATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCAAAGAGCCTGTCATTGCCGGCGCCACCATaaagtttgtcgttg  >  1:226947/2‑237 (MQ=255)                                                                                                                                          atTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCTTTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCaa                                           >  1:760071/1‑204 (MQ=255)                                                                                                                                             ccttACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAAc                                                                                                             >  1:868992/3‑135 (MQ=255)                                                                                                                                             ccttACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATgg                                                                                         >  1:890113/3‑155 (MQ=255)                                                                                                                                                        aCATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCAAAGAGCCtgtcattgcc                           >  1:669250/1‑197 (MQ=255)                                                                                                                                                         cATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCAttccccccttcta                                                                                              <  1:574100/138‑12 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                    TGTTACTGCTGTTCTATTATTTGTTTTCAATAGTAGATCATTGCCGTTACGCTCAAATGACACATCTTTGAGATTGATATTTGCTAATGATAATTTATCTTTATCACCGCTATGTTCCGTAATAGTGTGGTGCCCATATTCTTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGGTCATCGCCTTCTCCACCGTCAAGTAAATCATCTCCTTGGTTTCCAAACAACTTATCATTGCCACTTCCACCTCTTAGAATATCGGAACCATTGCCTCCCTCTAAGTGGTCGTCGCCTTCTCCACCGTCAAGATAGTTGTTGCCATATTCACCAAAGAGCCTATCGTTACCGGCACCACCATAGAGCTTATCATTA  >  1/1821501‑1821882

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑