Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,820,583	T→C	intergenic (+38683/+14801)	unknown → / ← unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,820,583	0	T	C	100.0%	160.9 / NA	55	intergenic (+38683/+14801)	unknown/unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (37/18);  total (37/18)

AGACTACAATAGCAAGAGCCACTGTAATTACATTCAATGTTGAAAATCCACGGTGAACCAGGACTTTATCCATCACGACTTGAAAAAAGAGTGGTGTAATCAGTGCAAAAATTTGCAAAAATATAGAAACGATTAACGTTTCTATAAAAATTTTGCGGTATTTAATTACGGCAGGAATAAACCAGGTAAAGTCGAATTTTGCTAAATTTCCTACAATAGATGCGCGTGATGCAATTAAAATCACCTTGCTTTGATAACGTTCTTCAAATTCATTTTTGTCTAAAACAATAGGATTTTTTTGAATTAGATCAAAAATGAGGTAACGATCGGTTTCTTGATCTATTTTTAATAAAATAAAGTGTTCACCATCATCACGCCAAGCCAATGCCGGTAAATGTAAAAAAGGCAATCTGTCAACTGTTTTGTTCGCAGTTCTTACCTTTAATCCAAGTGATTTTGCTG  >  1/1820349‑1820810                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                    aGACTACAATAGCAAGAGCCACTGTAATCACATTCAATGTTGAAAATCCACGGTGAACCAAGACCTTATCCATCACGACTTGAAAAAAGAGCGGTGTAATCAGTGCAAAAATTTGCAAAAATATAGAAACGATTAACGTTTCTATAAAAATTTTGCGGTATTTAATTACAGCAGGAATAAACCAGGTAAAATCGAATTTTGCTAAATTTCCTACAATAGATGCGCGTGACGCAACTAAAATCACCTTGCtt                                                                                                                                                                                                                     >  1:132698/1‑251 (MQ=255)  gACTACAATAGCAAGAGCCACTGTAATCACATTCAATGTTGAAAATCCACGGTGAACCAAGACCTTATCCATCACGACTTGAAAAAAGAGCGGTGTAATCAGTGCAAAAATTTGCAAAAATATAGAAACGATTAACGTTTCTATAAAAATTTTGCGGTATTTAATTACAGCAGGAATAAACCAGGTAAAATCGAATTTTGCTAAATTTCCTACAATAGATGCGCGTGACGCAACTAAAATCACCTTGCttt                                                                                                                                                                                                                    >  1:560011/1‑251 (MQ=255)  gACTACAATAGCAAGAGCCACTGTAATCACATTCAATGTTGAAAATCCACGGTGAACCAAGACCTTATCCATCACGACTTGAAAAAAGAGCGGTGTAATCAGTGCAAAAATTTGCAAAAATATAGAAACGATTAACGTTTCTATAAAAATTTTGCGGTATTTAATTACAGCAGGAATAAACCAGGTAAAATCGAATTTTGCTAAATTTCCTACAATAGATGCGCGTGACGCAACTAAAATCACCTTGCttt                                                                                                                                                                                                                    >  1:227416/1‑251 (MQ=255)    cTACAATAGCAAGAGCCACTGTAATCACATTCAATGTTGAAAATCCACGGTGAACCAAGACCTTATCCATCACGACTTGAAAAAAGAGCGGTGTAATCAGTGCAAAAATTTGCAAAAATATAGAAACGATTAACGTTTCTATAAAAATTTTGCGGTATTTAATTACAGCAGGAATAAACCAGGTAAAATCGAATTTTGCTAAATTTCCTACAATAGATGCGCGTGACGCAACTAAAATc                                                                                                                                                                                                                              >  1:829824/1‑239 (MQ=255)    cTACAATAGCAAGAGCCACTGTAATCACATTCAATGTTGAAAATCCACGGTGAACCAAGACCTTATCCATCACGACTTGAAAAAAGAGCGGTGTAATCAGTGCAAAAATTTGCAAAAATATAGAAACGATTAACGTTTCTATAAAAATTTTGCGGTATTTAATTACAGCAGGAATAAACCAGGTAAAATCGAATTTTGCTAAATTTCCTACAATAGATGCGCGTGACGCAACTAAAATCACCTTGCTTTGa                                                                                                                                                                                                                  >  1:349983/1‑251 (MQ=255)            gCAAGAGCCACTGTAATCACATTCAATGTTGAAAATCCACGGTGAACCAAGACCTTATCCATCACGACTTGAAAAAAGAGCGGTGTAATCAGTGCAAAAATTTGCAAAAATATAGAAACGATTAACGTTTCTATAAAAATTTTGCGGTATTTAATTACAGCAGGAATAAACCAGGTAAAATCGAATTTTGCTAAATTTCCTACAATAGATGCGCGTGACGCAACTAAAATCACCTTGCTTTGATAACGtt                                                                                                                                                                                                           >  1:98813/1‑250 (MQ=255)                      cTGTAATCACATTCAATGTTGAAAATCCACGGTGAACCAAGACCTTATCCATCACGACTTGAAAAAAGAGCGGTGTAATCAGTGCAAAAATTTGCAAAAATATAGAAACGATTAACGTTTCTATAAAAATTTTGCGGTATTTAATTACAGCAGGAATAAACCAGGTAAAATCGAATTTTGCTAAATTTCCTACAATAGATGCGCGTGACGCAACTAAAATCACCTTGCTTTGATAACGTTCTTCAAATTCa                                                                                                                                                                                                >  1:702999/1‑251 (MQ=255)                      aggTAATCACATTCAATGTTGAAAATCCACGGTGAACCAAGACCTTATCCATCACGACTTGAAAAAAGAGCGGTGTAATCAGTGCAAAAATTTGCAAAAATATAGAAACGATTAACGTTTCTATAAAAATTTTGCGGTATTTAATTACAGCAGGAATAAACCAGGTAAAATCGAATTTTGCTAAATTTCCTACAATAGATGCGCGTGACGCAACTAAAATCACCTTGCTTTGATAACGTTCTTCAAATTCa                                                                                                                                                                                                >  1:47721/3‑251 (MQ=255)                                    aaTGTTGAAAATCCACGGTGAACCAAGACCTTATCCATCACGACTTGAAAAAAGAGCGGTGTAATCAGTGCAAAAATTTGCAAAAATATAGAAACGATTAACGTTTCTATAAAAATTTTGCGGTATTTAATTACAGCAGGAATAAACCAGGTAAAATCGAATTTTGCTAAATTTCCTACAATAGATGCGCGTGACGCAACTAAAATCACCTTGCTTTGATAACGTTCTTCAAATTCATTTTTGTCTAAAAc                                                                                                                                                                                  <  1:724083/251‑1 (MQ=255)                                                   cGGTGAACCAAGACCTTATCCATCACGACTTGAAAAAAGAGCGGTGTAATCAGTGCAAAAATTTGCAAAAATATAGAAACGATTAACGTTTCTATAAAAATTTTGCGGTATTTAATTACAGCAGGAATAAACCAGGTAAAATCGAATTTTGCTAAATTTCCTACAATAGATGCGCGTGACGCAACTAAAATCACCTTGCTTTGATAACGTTCTTCAAATTCATTTTTGTCTAAAAc                                                                                                                                                                                  >  1:598554/1‑236 (MQ=255)                                                       gAACCAAGACCTTATCCATCACGACTTGAAAAAAGAGCGGTGTAATCAGTGCAAAAATTTGCAAAAATATAGAAACGATTAACGTTTCTATAAAAATTTTGCGGTATTTAATTACAGCAGGAATAAACCAGGTAAAATCGAATTTTGCTAAATTTCCTACAATAGATGCGCGTGACGCAACTAAAATCACCTTGCTTTGATAACGTTCTTCAAATTCATTTTTGTCTAAAACAATAGGATTTTTTTGCAtt                                                                                                                                                               >  1:333185/1‑251 (MQ=255)                                                        aaCCAAGACCTTATCCATCACGACTTGAAAAAAGAGCGGTGTAATCAGTGCAAAAATTTGCAAAAATATAGAAACGATTAACGTTTCTATAAAAATTTTGCGGTATTTAATTACAGCAGGAATAAACCAGGTAAAATCGAATTTTGCTAAATTTCCTACAATAGATGCGCGTGACGCAACTAAAATCACCTTGCTTTGATAACGt                                                                                                                                                                                                            >  1:683780/1‑205 (MQ=255)                                                         aCCAAGACCTTATCCATCACGACTTGAAAAAAGAGCGGTGTAATCAGTGCAAAAATTTGCAAAAATATAGAAACGATTAACGTTTCTATAAAAATTTTGCGGTATTTAATTACAGCAGGAATAAACCAGGTAAAATCGAATTTTGCTAAATTTCCTACAATAGATGCGCGTGACGCAACTAAAATCACCTTGCTTTGATAACGTTCTTCAAATTCATTTTTGTCTAAAACAATAGGATTTTTTTGCATTAg                                                                                                                                                             >  1:683547/1‑251 (MQ=255)                                                           caagaccttATCCATCACGACTTGAAAAAAGAGCGGTGTAATCAGTGCAAAAATTTGCAAAAATATAGAAACGATTAACGTTTCTATAAAAATTTTGCGGTATTTAATTACAGCAGGAATAAACCAGGTAAAATCGAATTTTGCTAAATTTCCTACAATAGATGCGCGTGACGCAACTAAAATCACCTTGCTTTGATAACGTTCTTCAAATTCATTTTTGTCTAAAACAATAGGATTTTTTTGCATTAGAt                                                                                                                                                           <  1:314564/244‑1 (MQ=255)                                                                 cttATCCATCACGACTTGAAAAAAGAGCGGTGTAATCAGTGCAAAAATTTGCAAAAATATAGAAACGATTAACGTTTCTATAAAAATTTTGCGGTATTTAATTACAGCAGGAATAAACCAGGTAAAATCGAATTTTGCTAAATTTCCTACAATAGATGCGCGTGACGCAACTAAAATCACCTTGCTTTGATAACGTTCTTCAAATTCATTTTTGTCTAAAACAATAGGATTTTTTTGCATTAGATCaaaaa                                                                                                                                                     <  1:497234/250‑1 (MQ=255)                                                                              aCTTGAAAAAAGAGCGGTGTAATCAGTGCAAAAATTTGCAAAAATATAGAAACGATTAACGTTTCTATAAAAATTTTGCGGTATTTAATTACAGCAGGAATAAACCAGGTAAAATCGAATTTTGCTAAATTTCCTACAATAGATGCGCGTGACGCAACTAAAATCACCTTGCTTTGATAACGTTCTTCAAATTCATTTTTGTCTAAAACAATAGGATTTTTTTGCATTAGATCAAAAATGAGGTAACGAt                                                                                                                                         <  1:72530/250‑1 (MQ=255)                                                                               cTTGAAAAAAGAGCGGTGTAATCAGTGCAAAAATTTGCAAAAATATAGAAACGATTAACGTTTCTATAAAAATTTTGCGGTATTTAATTACAGCAGGAATAAACCAGGTAAAATCGAATTTTGCTAAATTTCCTACAATAGATGCGCGTGACGCAACTAAAATCACCTTGCTTTGATAACGTTCTTCAAATTCATTTTTGTCTAAAACAATAGGATTTTTTTGCATTAGATCAAAAATGAGGTAACGATCg                                                                                                                                       >  1:414826/1‑251 (MQ=255)                                                                                  gAAAAAAGAGCGGTGTAATCAGTGCAAAAATTTGCAAAAATATAGAAACGATTAACGTTTCTATAAAAATTTTGCGGTATTTAATTACAGCAGGAATAAACCAGGTAAAATCGAATTTTGCTAAATTTCCTACAATAGATGCGCGTGACGCAACTAAAATCACCTTGCTTTGATAACGTTCTTCAAATTCATTTTTGTCTAAAACAATAGGATTTTTTTGCATTAGATCAAAAATGAGGTAACGATCGGtt                                                                                                                                    >  1:83480/1‑251 (MQ=255)                                                                                             ggtgTAATCAGTGCAAAAATTTGCAAAAATATAGAAACGATTAACGTTTCTATAAAAATTTTGCGGTATTTAATTACAGCAGGAATAAACCAGGTAAAATCGAATTTTGCTAAATTTCCTACAATAGATGCGCGTGACGCAACTAAAATCACCTTGCTTTGATAACGttcttc                                                                                                                                                                                                       <  1:598455/173‑1 (MQ=255)                                                                                             ggtgTAATCAGTGCAAAAATTTGCAAAAATATAGAAACGATTAACGTTTCTATAAAAATTTTGCGGTATTTAATTACAGCAGGAATAAACCAGGTAAAATCGAATTTTGCTAAATTTCCTACAATAGATGCGCGTGACGCAACTAAAATCACCTTGCTTTGATAACGTTCTTCAAATTCATTTTTGTCTAAAACAATAGGATTTTTTTGCATTAGATCAAAAATGAGGTAACGATCGGTTTCTTGATCTAt                                                                                                                         >  1:538728/1‑251 (MQ=255)                                                                                              gtgtAATCAGTGCAAAAATTTGCAAAAATATAGAAACGATTAACGTTTCTATAAAAATTTTGCGGTATTTAATTACAGCAGGAATAAACCAGGTAAAATCGAATTTTGCTAAATTTCCTACAATAGATGCGCGTGACGCAACTAAAATCACCTTGCTTTGATAACGTTCTTCAAATTCAtttt                                                                                                                                                                                            >  1:14381/1‑183 (MQ=255)                                                                                              gtgtAATCAGTGCAAAAATTTGCAAAAATATAGAAACGATTAACGTTTCTATAAAAATTTTGCGGTATTTAATTACAGCAGGAATAAACCAGGTAAAATCGAATTTTGCTAAATTTCCTACAATAGATGCGCGTGACGCAACTAAAATCACCTTGCTTTGATAACGTTCTTCAAATTCATTTTTGTCTAAAACAATAg                                                                                                                                                                             <  1:671927/198‑1 (MQ=255)                                                                                              gtgtAATCAGTGCAAAAATTTGCAAAAATATAGAAACGATTAACGTTTCTATAAAAATTTTGCGGTATTTAATTACAGCAGGAATAAACCAGGTAAAATCGAATTTTGCTAAATTTCCTACAATAGATGCGCGTGACGCAACTAAAATCACCTTGCTTTGATAACGTTCTTCAAATTCATTTTTGTCTAAAACAATAGGATTTTTTTGCATTAGATCAAAAATGAGGTAACGATCGGTTTCTTGATCTAtt                                                                                                                        >  1:252514/1‑251 (MQ=255)                                                                                              gtgtAATCAGTGCAAAAATTTGCAAAAATATAGAAACGATTAACGTTTCTATAAAAATTTTGCGGTATTTAATTACAGCAGGAATAAACCAGGTAAAATCGAATTTTGCTAAATTTCCTACAATAGATGCGCGTGACGCAACTAAAATCACCTTGCTTTGATAACGTTCTTCAAATTCATTTTTGTCTAAAACAATAGGATTTTTTTGCATTAGATCAAAAATGAGGTAACGATCGGTTTCTTGATCTAtt                                                                                                                        >  1:656098/1‑251 (MQ=255)                                                                                                       gTGCAAAAATTTGCAAAAATATAGAAACGATTAACGTTTCTATAAAAATTTTGCGGTATTTAATTACAGCAGGAATAAACCAGGTAAAATCGAATTTTGCTAAATTTCCTACAATAGATGCGCGTGACGCAACTAAAATCACCTTGCTTTGATAACGTTCTTCAAATTCATTTTTGTCTAAAACAATAGGATTTTTTTGCATTAGATCAAAAATGAgg                                                                                                                                                <  1:343534/218‑1 (MQ=255)                                                                                                       gTGCAAAAATTTGCAAAAATATAGAAACGATTAACGTTTCTATAAAAATTTTGCGGTATTTAATTACAGCAGGAATAAACCAGGTAAAATCGAATTTTGCTAAATTTCCTACAATAGATGCGCGTGACGCAACTAAAATCACCTTGCTTTGATAACGTTCTTCAAATTCATTTTTGTCTAAAACAATAGGATTTTTTTGCATTAGATCAAAAATGAGGTAACGATCGGTTTCTTGATCTATTTTCaataaa                                                                                                               >  1:849964/1‑251 (MQ=255)                                                                                                       gTGCAAAAATTTGCAAAAATATAGAAACGATTAACGTTTCTATAAAAATTTTGCGGTATTTAATTACAGCAGGAATAAACCAGGTAAAATCGAATTTTGCTAAATTTCCTACAATAGATGCGCGTGACGCAACTAAAATCACCTTGCTTTGATAACGTTCTTCAAATTCATTTTTGTCTAAAACAATAGGATTTTTTTGCATTAGATCAAAAATGAGGTAACGATCGGTTTCTTGATCTATTTTCaataaa                                                                                                               >  1:681118/1‑251 (MQ=255)                                                                                                       gTGCAAAAATTTGCAAAAATATAGAAACGATTAACGTTTCTATAAAAATTTTGCGGTATTTAATTACAGCAGGAATAAACCAGGTAAAATCGAATTTTGCTAAATTTCCTACAATAGATGCGCGTGACGCAACTAAAATCACCTTGCTTTGATAACGTTCTTCAAATTCATTTTTGTCTAAAACAATAGGATTTTTTTGCATTAGATCAAAAATGAGGTAACGATCGGTTTCTTGATCTATTTTCaataaa                                                                                                               >  1:686088/1‑251 (MQ=255)                                                                                                           aaaaaTTTGCAAAAATATAGAAACGATTAACGTTTCTATAAAAATTTTGCGGTATTTAATTACAGCAGGAATAAACCAGGTAAAATCGAATTTTGCTAAATTTCCTACAATAGATGCGCGTGACGCAACTAAAATCACCTTGCTTTGATAACGTTCTTCAAATTCATTTTTGTCTAAAACAATAGGATTTTTTTGCATTAGATCAAAAATGAGGTAACGATCGGTTTCTTGATCTATTTTCaataaaataa                                                                                                           <  1:567909/251‑1 (MQ=255)                                                                                                                tttGCAAAAATATAGAAACGATTAACGTTTCTATAAAAATTTTGCGGTATTTAATTACAGCAGGAATAAACCAGGTAAAATCGAATTTTGCTAAATTTCCTACAATAGATGCGCGTGACGCAACTAAAATCACCTTGCTTTGATAACGTTCTTCAAATTCATTTTTGTCTAAAACAATAGGATTTTTTTGCATTAGATCAAAAATGAGGTAACGATCGGTTTCTTGATCTATTTTCAATAAAATAAAgtgg                                                                                                      <  1:195016/251‑2 (MQ=255)                                                                                                                                  cGATTAACGTTTCTATAAAAATTTTGCGGTATTTAATTACAGCAGGAATAAACCAGGTAAAATCGAATTTTGCTAAATTTCCTACAATAGATGCGCGTGACGCAACTaaaa                                                                                                                                                                                                                                <  1:87515/111‑1 (MQ=255)                                                                                                                                  cGATTAACGTTTCTATAAAAATTTTGCGGTATTTAATTACAGCAGGAATAAACCAGGTAAAATCGAATTTTGCTAAATTTCCTACAATAGATGCGCGTGACGCAACTAAAATCACCTTGCTTTGATAACGTTCTTCAAATTCATTTTTGTCTAAAACAATAGGATTTTTTTGCATTAGATCAAAAATGAGGTAACGATCGGTTTCTTGATCTATTTTCAATAAAATAAAGTGGTCACCATCATCACgccaa                                                                                    >  1:885193/1‑251 (MQ=255)                                                                                                                                           tttCTATAAAAATTTTGCGGTATTTAATTACAGCAGGAATAAACCAGGTAAAATCGAATTTTGCTAAATTTCCTACAATAGATGCGCGTGACGCAACTAAAATCACCTTGCTTTGATAACGTTCTTCAAATTCATTTTTGTCTAAAACAATAGGATTTTTTTGCATTAGATCAAAAATGAGGTAACGATCGGTTTCTTGATCTATTTTCAATAAAATAAAGTGGTCACCATCATCACGCCAAGCCAAtac                                                                            <  1:93367/250‑3 (MQ=255)                                                                                                                                                              gTATTTAATTACAGCAGGAATAAACCAGGTAAAATCGAATTTTGCTAAATTTCCTACAATAGATGCGCGTGACGCAACTAAAATCACCTTGCTTTGATAACGTTCTTCAAATTCATTTTTGTCTAAAACAATAGGAt                                                                                                                                                                          >  1:862863/1‑137 (MQ=255)                                                                                                                                                              gTATTTAATTACAGCAGGAATAAACCAGGTAAAATCGAATTTTGCTAAATTTCCTACAATAGATGCGCGTGACGCAACTAAAATCACCTTGCTTTGATAACGTTCTTCAAATTCATTTTTGTCTAAAACAATAGGATTTTTTTGCATTAGATCAAAAATg                                                                                                                                                   >  1:600105/1‑160 (MQ=255)                                                                                                                                                              gTATTTAATTACAGCAGGAATAAACCAGGTAAAATCGAATTTTGCTAAATTTCCTACAATAGATGCGCGTGACGCAACTAAAATCACCTTGCTTTGATAACGTTCTTCAAATTCATTTTTGTCTAAAACAATAGGATTTTTTTGCATTAGATCAAAAATGAGGTAACGATCGGtt                                                                                                                                    >  1:472839/1‑175 (MQ=255)                                                                                                                                                              gTATTTAATTACAGCAGGAATAAACCAGGTAAAATCGAATTTTGCTAAATTTCCTACAATAGATGCGCGTGACGCAACTAAAATCACCTTGCTTTGATAACGTTCTTCAAATTCATTTTTGTCTAAAACAATAGGATTTTTTTGCATTAGATCAAAAATGAGGTAACGATCGGTTTCTTGATCTATTTTCAATAAAATAAAGTGGTCACCATCATCACgccaagcc                                                                                 <  1:481532/226‑1 (MQ=255)                                                                                                                                                              gTATTTAATTACAGCAGGAATAAACCAGGTAAAATCGAATTTTGCTAAATTTCCTACAATAGATGCGCGTGACGCAACTAAAATCACCTTGCTTTGATAACGTTCTTCAAATTCATTTTTGTCTAAAACAATAGGATTTTTTTGCATTAGATCAAAAATGAGGTAACGATCGGTTTCTTGATCTATTTTCAATAAAATAAAGTGGTCACCATCATCACGCCAAGCCAATACCGGTAAATGTAAAAAAggta                                                        >  1:431418/1‑249 (MQ=255)                                                                                                                                                                aTTTAATTACAGCAGGAATAAACCAGGTAAAATCGAATTTTGCTAAATTTCCTACAATAGATGCGCGTGACGCAACTAAAATCACCTTGCTTTGATAACGTTCTTCAAATTCATTTTTGTCTAAAACAATAGGATTTTTTTGCATTAGATCAAAAATGAGGTAACGATCGGTTTCTTGATCTATTTTCAATAAAATAAAGTGGTCACCATCATCACgccaagc                                                                                  >  1:571277/1‑223 (MQ=255)                                                                                                                                                                aTTTAATTACAGCAGGAATAAACCAGGTAAAATCGAATTTTGCTAAATTTCCTACAATAGATGCGCGTGACGCAACTAAAATCACCTTGCTTTGATAACGTTCTTCAAATTCATTTTTGTCTAAAACAATAGGATTTTTTTGCATTAGATCAAAAATGAGGTAACGATCGGTTTCTTGATCTATTTTCAATAAAATAAAGTGGTCACCATCATCACGCCAAGCCAAtac                                                                            <  1:618817/229‑3 (MQ=255)                                                                                                                                                                   tAATTACAGCAGGAATAAACCAGGTAAAATCGAATTTTGCTAAATTTCCTACAATAGATGCGCGTGACGCAACTAAAATCACCTTGCTTTGATAACGTTCTTCAAATTCATTTTTGTCTAAAACAATAGGATTTTTTTGCATTAGATCAAAAATGAGGTAACGATCGGTTTCTTGATCTATTTTcaat                                                                                                                  >  1:778095/1‑188 (MQ=255)                                                                                                                                                                         cagCAGGAATAAACCAGGTAAAATCGAATTTTGCTAAATTTCCTACAATAGATGCGCGTGACGCAACTAAAATCACCTTGCTTTGATAACGTTCTTCAAATTCATTTTTGTCTAAAACAATAGGATTTTTTTGCATTAGATCAAAAATGAGGTAACGATCGGTTTCTTg                                                                                                                               >  1:322745/3‑169 (MQ=255)                                                                                                                                                                            cAGGAATAAACCAGGTAAAATCGAATTTTGCTAAATTTCCTACAATAGATGCGCGTGACGCAACTAAAATCACCTTGCTTTGATAACGTTCTTCAAATTCATTTTTGTCTAAAACAATAGGATTTTTTTGCATTAGATCAAAAATGAGGTAACGATCGGTTTCTTGATCTATTTTCAATAAAATAAAGTGGTCACCATCATCACGCCAAGCCAATACCGGTAAATGTAAAAAAGGTAATCTATCAATTGt                                           <  1:570325/250‑1 (MQ=255)                                                                                                                                                                              ggAATAAACCAGGTAAAATCGAATTTTGCTAAATTTCCTACAATAGATGCGCGTGACGCAACTAAAATCACCTTGCTTTGATAACGTTCTTCAAATTCATTTTTGTCTAAAAc                                                                                                                                                                                  >  1:553291/1‑113 (MQ=255)                                                                                                                                                                              ggAATAAACCAGGTAAAATCGAATTTTGCTAAATTTCCTACAATAGATGCGCGTGACGCAACTAAAATCACCTTGCTTTGATAACGTTCTTCAAATTCATTTTTGTCTAAAACAATAGGATTTTTTTGCATTAGATCAAAAATGAGGTAACGATCGGTTTCTTGATCTATTTTCAATAAAATAAAgt                                                                                                        >  1:472245/1‑187 (MQ=255)                                                                                                                                                                              ggAATAAACCAGGTAAAATCGAATTTTGCTAAATTTCCTACAATAGATGCGCGTGACGCAACTAAAATCACCTTGCTTTGATAACGTTCTTCAAATTCATTTTTGTCTAAAACAATAGGATTTTTTTGCATTAGATCAAAAATGAGGTAACGATCGGTTTCTTGATCTATTTTCAATAAAATAAAGTGGTCACCATCATCACGCCAAGCCAATACCGGTAAATGTAAAAAAGGTAATCTATCAATTGtttt                                        >  1:541494/1‑251 (MQ=255)                                                                                                                                                                               gAATAAACCAGGTAAAATCGAATTTTGCTAAATTTCCTACAATAGATGCGCGTGACGCAACTAAAATCACCTTGCTTTGATAACGTTCTTCAAATTCATTTTTGTCTAAAACAATAGGATTTTTTTGCATTAGATCAAAAATGAGGTAACGATCGGTTTCTTGATCTATTTTCAATAAAATAAAGTGGTCACCATCATCACGCCAAGCCAAtac                                                                            <  1:814704/214‑3 (MQ=255)                                                                                                                                                                                    aaCCAGGTAAAATCGAATTTTGCTAAATTTCCTACAATAGATGCGCGTGACGCAACTAAAATCACCTTGCTTTGATAACGTTCTTCAAATTCATTTTTGTCTAAAACAATAGGATTTTTTTGCATTAGATCAAAAATGAGGTAACGATCGGTTTCTTGATCTATTTTCaataaaat                                                                                                             >  1:847325/1‑176 (MQ=255)                                                                                                                                                                                              aatCGAATTTTGCTAAATTTCCTACAATAGATGCGCGTGACGCAACTAAAATCACCTTGCTTTGATAACGTTCTTCAAATTCATTTTTGTCTAAAACAATAGGATTTTTTTGCATTAGATCAAAAATGAGGTAACGATCGGTTTCTTGATCTATTTTCAATAAAATAAAGTGGTCACCATCATCACGCCAAGCCAATACCGGTAAATGTAAAAAAGGTAATCTATCAATTGTTTTGTTCGTAGTTCTTa                          >  1:636608/3‑249 (MQ=255)                                                                                                                                                                                                                 tCCTACAATAGATGCGCGTGACGCAACTAAAATCACCTTGCTTTGATAACGTTCTTCAAATTCATTTTTGTCTAAAACAATAGGATTTTTTTGCATTAGATCAAAAATGAGGTAACGATCGGTTTCTTGATCTATTTTCaataaaataa                                                                                                           >  1:481947/1‑149 (MQ=255)                                                                                                                                                                                                                      cAATAGATGCGCGTGACGCAACTAAAATCACCTTGCTTTGATAACGTTCTTCAAATTCATTTTTGTCTAAAACAATAGGATTTTTTTGCATTAGATCAAAAATGAGGTAACGATCGGTTTCTTGATCTATTTTcaat                                                                                                                  <  1:58147/137‑1 (MQ=255)                                                                                                                                                                                                                      cAATAGATGCGCGTGACGCAACTAAAATCACCTTGCTTTGATAACGTTCTTCAAATTCATTTTTGTCTAAAACAATAGGATTTTTTTGCATTAGATCAAAAATGAGGTAACGATCGGTTTCTTGATCTATTTTCAATAAAATAAAgtgg                                                                                                      <  1:532995/149‑2 (MQ=255)                                                                                                                                                                                                                      cAATAGATGCGCGTGACGCAACTAAAATCACCTTGCTTTGATAACGTTCTTCAAATTCATTTTTGTCTAAAACAATAGGATTTTTTTGCATTAGATCAAAAATGAGGTAACGATCGGTTTCTTGATCTATTTTCAATAAAATAAAGTGGTCACCATCATCACGCCAAGCCAATACCGGTAAATGTAAAAAAGGTAATCTATCAATTGTTTTGTTCGTAGTTCTTACTTTTAATc                 >  1:19746/1‑234 (MQ=255)                                                                                                                                                                                                                      cAATAGATGCGCGTGACGCAACTAAAATCACCTTGCTTTGATAACGTTCTTCAAATTCATTTTTGTCTAAAACAATAGGATTTTTTTGCATTAGATCAAAAATGAGGTAACGATCGGTTTCTTGATCTATTTTCAATAAAATAAAGTGGTCACCATCATCACGCCAAGCCAATACCGGTAAATGTAAAAAAGGTAATCTATCAATTGTTTTGTTCGTAGTTCTTACTTTTAATCCAAGTGATCTtgctg  <  1:339897/249‑1 (MQ=255)                                                                                                                                                                                                                        aTAGATGCGCGTGACGCAACTAAAATCACCTTGCTTTGATAACGTTCTTCAAATTCATTTTTGTCTAAAACAATAGGATTTTTTTGCATTAGATCAAAAATGAGGAAACGATCGGTTTCTTGATCTATTTTCAATAAAATAAAGTGGTCACCATCATCACGCCAAGCCAATACCGGTAAATGTAAAAAAggt                                                         >  1:569858/1‑191 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                    AGACTACAATAGCAAGAGCCACTGTAATTACATTCAATGTTGAAAATCCACGGTGAACCAGGACTTTATCCATCACGACTTGAAAAAAGAGTGGTGTAATCAGTGCAAAAATTTGCAAAAATATAGAAACGATTAACGTTTCTATAAAAATTTTGCGGTATTTAATTACGGCAGGAATAAACCAGGTAAAGTCGAATTTTGCTAAATTTCCTACAATAGATGCGCGTGATGCAATTAAAATCACCTTGCTTTGATAACGTTCTTCAAATTCATTTTTGTCTAAAACAATAGGATTTTTTTGAATTAGATCAAAAATGAGGTAACGATCGGTTTCTTGATCTATTTTTAATAAAATAAAGTGTTCACCATCATCACGCCAAGCCAATGCCGGTAAATGTAAAAAAGGCAATCTGTCAACTGTTTTGTTCGCAGTTCTTACCTTTAATCCAAGTGATTTTGCTG  >  1/1820349‑1820810

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑