Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,820,185	C→T	intergenic (+38285/+15199)	unknown → / ← unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,820,185	0	C	T	100.0%	237.1 / NA	80	intergenic (+38285/+15199)	unknown/unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (38/42);  total (38/42)

AACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGACTAATAAAAACAGACCAAATGACATAACAAGGTAAAGAACCGAGTACAACTAATGTTAATTTTGGGCTGTAATACCACATTACAGCGAAGAAAATAAAAGAGAATAACAAGTCTAAAATAGAGGTAAGTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAACTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAATGACGAAAAAGCCTTGCTCCCAATTCGACATCAATACGACTTGTACTATGAGCAAAAACATAGGTTCTCAAGCCTCCCAAGATTATTTCAAATAAGACTACAATAGCAAGAGCCACTGTAATTACATTCAATGTTGAAAATCCACGGTGAACCAGGACTTTATCCATCACGACTT  >  1/1819954‑1820429                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                     aacaaGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGACTAATAAAAACAGACCAAATGACATAACAAGGTAAAGAACCGAGTACAACTAATGTTAATTTTGGGCTGTAATACCACATAACGGCGAAGAAAATAAAAGAGAATAACAAGTCTAAAATAGAGGTAAGTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCg                                                                                                                                                                                                                                   >  1:175926/1‑251 (MQ=255)    aaGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGACTAATAAAAACAGACCAAATGACATAACAAGGTAAAGAACCGAGTACAACTAATGTTAATTTTGGGCTGTAATACCACATAACGGCGAAGAAAACAAAAGAGAATAACAAGTCTAAAATAGAGGTAAGTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTAt                                                                                                                                                                                                                                <  1:11470/251‑1 (MQ=255)     aGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGACTAATAAAAACAGACCAAATGACATAACAAGGTAAAGAACCGAGTACAACTAATGTTAATTTTGGGCTGTAATACCACATAACGGCGAAGAAAATAAAAGAGAATAACAAGTCTAAAATAGAGGTAAGTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTAt                                                                                                                                                                                                                                >  1:872110/1‑250 (MQ=255)       aaaaGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGACTAATAAAAACAGACCAAATGACATAACAAGGTAAAGAACCGAGTACAACTAATGTTAATTTTGGGCTGTAATACCACATAACGGCGAAGAAAATAAAAGAGAATAACAAGTCTAAAATAGAGGTAAGTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATcc                                                                                                                                                                                                                              <  1:52198/250‑1 (MQ=255)          aGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGACTAATAAAAACAGACCAAATGACATAACAAGGTAAAGAACCGAGTACAACTAATGTTAATTTTGGGCTGTAATACCACATAACGGCGAAGAAAATAAAAGAGAATAACAAGTCTAAAATAGAGGTAAGTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCaa                                                                                                                                                                                                                          >  1:374923/1‑251 (MQ=255)                                         tcatcaAGCCGTCTCCTAAGAATAGGACTAATAAAAACAGACCAAATGACATAACAAGGTAAAGAACCGAGTACAACTAATGTTAATTTTGGGCTGTAATACCACATAACGGCGAAGAAAATAAAAGAGAATAACAAGTCTAAAATAGAGGTAAGTGCCTGCCCTGTTAGGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTaa                                                                                                                                                                                           >  1:441786/1‑251 (MQ=255)                                                  cGTCTCCTAAGAATAGGACTAATAAAAACAGACCAAATGACATAACAAGGTAAAGAACCGAGTACAACTAATGTTAATTTTGGGCTGTAATACCACATAACGGCGAAGAAAATAAAAGAGAATAACAAGTCTAAAATAGAGGTAAGTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCtaataa                                                                                                                                                                                  <  1:792446/251‑1 (MQ=255)                                                    tctcCTAAGAATAGGACTAATAAAAACAGACCAAATGACATAACAAGGTAAAGAACCGAGTACAACTAATGTTAATTTTGGGCTGTAATACCACATAACGGCGAAGAAAATAAAAGAGAATAACAAGTCTAAAATAGAGGTAAGTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCtaataagt                                                                                                                                                                                >  1:210027/1‑249 (MQ=255)                                                    tctcCTAAGAATAGGACTAATAAAAACAGACCAAATGACATAACAAGGTAAAGAACCGAGTACAACTAATGTTAATTTTGGGCTGTAATACCACATAACGGCGAAGAAAATAAAAGAGAATAACAAGTCTAAAATAGAGGTAAGTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCtaataag                                                                                                                                                                                 <  1:629861/250‑2 (MQ=255)                                                    tctcCTAAGAATAGGACTAATAAAAACAGACCAAATGACATAACAAGGTAAAGAACCGAGTACAACTAATGTTAATTTTGGGCTGTAATACCACATAACGGCGAAGAAAATAAAAGAGAATAACAAGTCTAAAATAGAGGTAAGTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCtaataag                                                                                                                                                                                 <  1:704047/250‑2 (MQ=255)                                                     ctcCTAAGAATAGGACTAATAAAAACAGACCAAATGACATAACAAGGTAAAGAACCGAGTACAACTAATGTTAATTTTGGGCTGTAATACCACATAACGGCGAAGAAAATAAAAGAGAATAACAAGTCTAAAATAGAGGTAAGTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCtaataagtg                                                                                                                                                                               >  1:506658/1‑248 (MQ=255)                                                       ccTAAGAATAGGACTAATAAAAACAGACCAAATGACATAACAAGGTAAAGAACCGAGTACAACTAATGTTAATTTTGGGCTGTAATACCACATAACGGCGAAGAAAATAAAAGAGAATAACAAGTCTAAAATAGAGGTAAGTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCtaataag                                                                                                                                                                                 <  1:549000/247‑2 (MQ=255)                                                            gAATAGGACTAATAAAAACAGACCAAATGACATAACAAGGTAAAGAACCGAGTACAACTAATGTTAATTTTGGGCTGTAATACCACATAACGGCGAAGAAAATAAAAGAGAATAACAAGTCTAAAATAGAGGTAAGTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCtaataag                                                                                                                                                                                 >  1:60680/1‑241 (MQ=255)                                                                      aataaAAACAGACCAAATGACATAACAAGGTAAAGAACCGAGTACAACTAATGTTAATTTTGGGCTGTAATACCACATAACGGCGAAGAAAATAAAAGAGAATAACAAGTCTAAAATAGAGGTAAGTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATccc                                                                                                                                                                                                                             >  1:736392/1‑188 (MQ=255)                                                                      aataaAAACAGACCAAATGACATAACAAGGTAAAGAACCGAGTACAACTAATGTTAATTTTGGGCTGTAATACCACATAACGGCGAAGAAAATAAAAGAGAATAACAAGTCTAAAATAGAGGTAAGTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAAc                                                                                                                                                                                                                         >  1:828595/1‑192 (MQ=255)                                                                      aataaAAACAGACCAAATGACATAACAAGGTAAAGAACCGAGTACAACTAATGTTAATTTTGGGCTGTAATACCACATAACGGCGAAGAAAATAAAAGAGAATAACAAGTCTAAAATAGAGGTAAGTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAAt                                                                                                                                                                                                        <  1:193101/209‑1 (MQ=255)                                                                      aataaAAACAGACCAAATGACATAACAAGGTAAAGAACCGAGTACAACTAATGTTAATTTTGGGCTGTAATACCACATAACGGCGAAGAAAATAAAAGAGAATAACAAGTCTAAAATAGAGGTAAGTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCt                                                                                                                                                               >  1:639711/1‑250 (MQ=255)                                                                      aataaAAACAGACCAAATGACATAACAAGGTAAAGAACCGAGTACAACTAATGTTAATTTTGGGCTGTAATACCACATAACGGCGAAGAAAATAAAAGAGAATAACAAGTCTAAAATAGAGGTAAGTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCt                                                                                                                                                               >  1:210169/1‑250 (MQ=255)                                                                      aataaAAACAGACCAAATGACATAACAAGGTAAAGAACCGAGTACAACTAATGTTAATTTTGGGCTGTAATACCACATAACGGCGAAGAAAATAAAAGAGAATAACAAGTCTAAAATAGAGGTAAGTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCt                                                                                                                                                               >  1:533878/1‑250 (MQ=255)                                                                      aataaAAACAGACCAAATGACATAACAAGGTAAAGAACCGAGTACAACTAATGTTAATTTTGGGCTGTAATACCACATAACGGCGAAGAAAATAAAAGAGAATAACAAGTCTAAAATAGAGGTAAGTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTc                                                                                                                                                              <  1:852227/251‑1 (MQ=255)                                                                                  ccAAATGACATAACAAGGTAAAGAACCGAGTACAACTAATGTTAATTTTGGGCTGTAATACCACATAACGGCGAAGAAAATAAAAGAGAATAACAAGTCTAAAATAGAGGTAAGTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACAt                                                                                                                                                  <  1:421804/251‑1 (MQ=255)                                                                                  ccAAATGACATAACAAGGTAAAGAACCGAGTACAACTAATGTTAATTTTGGGCTGTAATACCACATAACGGCGAAGAAAATAAAAGAGAATAACAAGTCTAAAATAGAGGTAAGTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACAt                                                                                                                                                  <  1:763013/251‑1 (MQ=255)                                                                                         aCATAACAAGGTAAAGAACCGAGTACAACTAATGTTAATTTTGGGCTGTAATACCACATAACGGCGAAGAAAATAAAAGAGAATAACAAGTCTAAAATAGAGGTAAGTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACg                                                                                                                                           >  1:633345/1‑251 (MQ=255)                                                                                                  ggTAAAGAACCGAGTACAACTAATGTTAATTTTGGGCTGTAATACCACATAACGGCGAAGAAAATAAAAGAGAATAACAAGTCTAAAATAGAGGTAAGTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACGACTTGtg                                                                                                                                    >  1:107874/1‑248 (MQ=255)                                                                                                   gTAAAGAACCGAGTACAACTAATGTTAATTTTGGGCTGTAATACCACATAACGGCGAAGAAAATAAAAGAGAATAACAAGTCTAAAATAGAGGTAAGTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCcgacg                                                                                                                                                                                                                   <  1:240322/169‑1 (MQ=255)                                                                                                   gTAAAGAACCGAGTACAACTAATGTTAATTTTGGGCTGTAATACCACATAACGGCGAAGAAAATAAAAGAGAATAACAAGTCTAAAATAGAGGTAAGTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCtaataag                                                                                                                                                                                 <  1:147114/203‑2 (MQ=255)                                                                                                   gTAAAGAACCGAGTACAACTAATGTTAATTTTGGGCTGTAATACCACATAACGGCGAAGAAAATAAAAGAGAATAACAAGTCTAAAATAGAGGTAAGTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGAc                                                                                                                                                    <  1:228556/232‑1 (MQ=255)                                                                                                   gTAAAGAACCGAGTACAACTAATGTTAATTTTGGGCTGTAATACCACATAACGGCGAAGAAAATAAAAGAGAATAACAAGTCTAAAATAGAGGTAAGTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACGACTTGTGCTa                                                                                                                                 >  1:717705/1‑251 (MQ=255)                                                                                                   gTAAAGAACCGAGTACAACTAATGTTAATTTTGGGCTGTAATACCACATAACGGCGAAGAAAATAAAAGAGAATAACAAGTCTAAAATAGAGGTAAGTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACGACTTGTGCTa                                                                                                                                 >  1:290539/1‑251 (MQ=255)                                                                                                   gTAAAGAACCGAGTACAACTAATGTTAATTTTGGGCTGTAATACCACATAACGGCGAAGAAAATAAAAGAGAATAACAAGTCTAAAATAGAGGTAAGTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACGACTTGTGCTa                                                                                                                                 >  1:715704/1‑251 (MQ=255)                                                                                                        gAACCGAGTACAACTAATGTTAATTTTGGGCTGTAATACCACATAACGGCGAAGAAAATAAAAGAGAATAACAAGTCTAAAATAGAGGTAAGTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACGACTTGTGCTATGAg                                                                                                                             >  1:416262/1‑250 (MQ=255)                                                                                                               gTACAACTAATGTTAATTTTGGGCTGTAATACCACATAACGGCGAAGAAAATAAAAGAGAATAACAAGTCTAAAATAGAGGTAAGTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCtaataag                                                                                                                                                                                 <  1:289540/191‑2 (MQ=255)                                                                                                                  cAACTAATGTTAATTTTGGGCTGTAATACCACATAACGGCGAAGAAAATAAAAGAGAATAACAAGTCTAAAATAGAGGTAAGTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACGACTTGtgct                                                                                                                                  <  1:501855/235‑4 (MQ=255)                                                                                                                   aaCTAATGTTAATTTTGGGCTGTAATACCACATAACGGCGAAGAAAATAAAAGAGAATAACAAGTCTAAAATAGAGGTAAGTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACGACTTGTGCTATGAGCAAACACATAgg                                                                                                                 <  1:777333/251‑1 (MQ=255)                                                                                                                    aCTAATGTTAATTTTGGGCTGTAATACCACATAACGGCGAAGAAAATAAAAGAGAATAACAAGTCTAAAATAGAGGTAAGTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCtaataag                                                                                                                                                                                 <  1:102076/186‑2 (MQ=255)                                                                                                                    aCTAATGTTAATTTTGGGCTGTAATACCACATAACGGCGAAGAAAATAAAAGAGAATAACAAGTATAAAATAGAGGTAAGTGCCTGCCCTGTTAAGAAATTGCGAATTTGATCTAATACACGAACACGAGCCACCGTATCCCCAAGCCGACGAGCTTCAAAATAAGAAATCGGTAAAACTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACGACTTGTGCTATGAGCAAACACATAGGt                                                                                                                <  1:616305/251‑1 (MQ=255)                                                                                                                                      cTGTAATACCACATAACGGCGAAGAAAATAAAAGAGAATAACAAGTCTAAAATAGAGGTAAGTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACGACTTGTGCTATGAGCAAACACATAGGt                                                                                                                >  1:719673/1‑233 (MQ=255)                                                                                                                                      cTGTAATACCACATAACGGCGAAGAAAATAAAAGAGAATAACAAGTCTAAAATAGAGGTAAGTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACGACTTGTGCTATGAGCAAACACATAGGTTCTCAGCCCCCCCAAGac                                                                                              <  1:761572/251‑2 (MQ=255)                                                                                                                                        gTAATACCACATAACGGCGAAGAAAATAAAAGAGAATAACAAGTCTAAAATAGAGGTAAGTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGt                                                                                                                                                                                             >  1:119235/1‑154 (MQ=255)                                                                                                                                        gTAATACCACATAACGGCGAAGAAAATAAAAGAGAATAACAAGTCTAAAATAGAGGTAAGTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACGACTTGTGCTATGAGCAAACACAt                                                                                                                    <  1:133972/227‑1 (MQ=255)                                                                                                                                        gTAATACCACATAACGGCGAAGAAAATAAAAGAGAATAACAAGTCTAAAATAGAGGTAAGTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACGACTTGTGCTATGAGCAAACACATAGGTTCTCAGCCCCCCCAAGacta                                                                                            >  1:406006/1‑248 (MQ=255)                                                                                                                                        gTAATACCACATAACGGCGAAGAAAATAAAAGAGAATAACAAGTCTAAAATAGAGGTAAGTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACGACTTGTGCTATGAGCAAACACATAGGTTCTCAGCCCCCCCAAGacta                                                                                            >  1:739869/1‑248 (MQ=255)                                                                                                                                        gTAATACCACATAACGGCGAAGAAAATAAAAGAGAATAACAAGTCTAAAATAGAGGTAAGTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACGACTTGTGCTATGAGCAAACACATAGGTTCTCAGCCCCCCCAAGacta                                                                                            >  1:507986/1‑248 (MQ=255)                                                                                                                                        gTAATACCACATAACGGCGAAGAAAATAAAAGAGAATAACAAGTCTAAAATAGAGGTAAGTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACGACTTGTGCTATGAGCAAACACATAGGTTCTCAGCCCCCCCAAGac                                                                                              <  1:670742/249‑2 (MQ=255)                                                                                                                                        gTAATACCACATAACGGCGAAGAAAATAAAAGAGAATAACAAGTCTAAAATAGAGGTAAGTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACGACTTGTGCTATGAGCAAACACATAGGTTCTCAGCCCCCCCAAGac                                                                                              <  1:201054/249‑2 (MQ=255)                                                                                                                                           aTACCACATAACGGCGAAGAAAATAAAAGAGAATAACAAGTCTAAAATAGAGGTAAGTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACGACTTGTGCTATGAGCAAACACATAGGTTCTCAGCCCCCCCAAGACTAttt                                                                                         >  1:83799/1‑251 (MQ=255)                                                                                                                                              ccACATAACGGCGAAGAAAATAAAAGAGAATAACAAGTCTAAAATAGAGGTAAGTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAAt                                                                                                                                                                                                        >  1:853109/1‑137 (MQ=255)                                                                                                                                              ccACATAACGGCGAAGAAAATAAAAGAGAATAACAAGTCTAAAATAGAGGTAAGTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCtaa                                                                                                                                                                                     <  1:622541/156‑1 (MQ=255)                                                                                                                                              ccACATAACGGCGAAGAAAATAAAAGAGAATAACAAGTCTAAAATAGAGGTAAGTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACGACTTGTGCTATGAGCAAACACATAGGTTCTCAGCCCCCCCAAGACTATTTCaa                                                                                      <  1:247381/251‑1 (MQ=255)                                                                                                                                              ccACATAACGGCGAAGAAAATAAAAGAGAATAACAAGTCTAAAATAGAGGTAAGTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACGACTTGTGCTATGAGCAAACACATAGGTTCTCAGCCCCCCCAAGACTATTTCaa                                                                                      >  1:58880/1‑251 (MQ=255)                                                                                                                                              ccACATAACGGCGAAGAAAATAAAAGAGAATAACAAGTCTAAAATAGAGGTAAGTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACGACTTGTGCTATGAGCAAACACATAGGTTCTCAGCCCCCCCAAGACTATTTCaa                                                                                      >  1:61930/1‑251 (MQ=255)                                                                                                                                                acataacggCGAAGAAAATAAAAGAGAATAACAAGTCTAAAATAGAGGTAAGTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCtaataa                                                                                                                                                                                  >  1:42626/9‑157 (MQ=255)                                                                                                                                                acataacggCGAAGAAAATAAAAGAGAATAACAAGTCTAAAATAGAGGTAAGTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACGACTTGTGCTATGAGCAAACACATAGGTTCTCAGCCCCCCCAAGACTATTTCAAAt                                                                                    <  1:724270/243‑1 (MQ=255)                                                                                                                                                acataacggCGAAGAAAATAAAAGAGAATAACAAGTCTAAAATAGAGGTAAGTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACGACTTGTGCTATGAGCAAACACATAGGTTCTCAGCCCCCCCAAGACTATTTCAAAt                                                                                    <  1:297267/243‑1 (MQ=255)                                                                                                                                                               aaaTAAAAGAGAATAACAAGTCTAAAATAGAGGTAAGTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACGACTTGTGCTATGAGCAAACACATAGGTTCTCAGCCCCCCCAAGACTATTTCAAATAAGACTACAATAGCa                                                                     <  1:761232/251‑1 (MQ=255)                                                                                                                                                                      agagAATAACAAGTCTAAAATAGAGGTAAGTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACGAc                                                                                                                                         <  1:385979/176‑1 (MQ=255)                                                                                                                                                                        agaATAACAAGTCTAAAATAGAGGTAAGTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACGACTTGTGCTATGAGCAAACACATAGGTTCTCAGCCCCCCCAAGACTATTTCAAATAAGACTACAATAGCAAGAGCCa                                                              >  1:838712/1‑249 (MQ=255)                                                                                                                                                                                        aaTAGAGGTAAGTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACGACTTGTGCTATGAGCAAACACATAGGTTCTCAGCCCCCCCAAGACTATTTCAAATAAGACTACAATAGCAAGAGCCACTGTAATCACATTCAATg                                            <  1:424440/251‑1 (MQ=255)                                                                                                                                                                                         aTAGAGGTAAGTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACGACTTGTGCTATGAGCAAACACATAGGTTCTCAGCCCCCCCAAGACTATTTCAAATAAGACTACAATAGCAAGAGCCACTGTAAtca                                                     <  1:333660/241‑3 (MQ=255)                                                                                                                                                                                         aTAGAGGTAAGTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACGACTTGTGCTATGAGCAAACACATAGGTTCTCAGCCCCCCCAAGACTATTTCAAATAAGACTACAATAGCAAGAGCCACTGTAATCACATTCAATGt                                           <  1:733920/251‑1 (MQ=255)                                                                                                                                                                                              ggTAAGTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACGACTTGTGCTATGAGCAAACACATAGGTTCTCAGCCCCCCCAAGACTATTTCAAATAAGACTACAATAGCAAGAGCCACTGTAATCACATTCAATGTTGaaa                                      >  1:756074/1‑251 (MQ=255)                                                                                                                                                                                              ggTAAGTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACGACTTGTGCTATGAGCAAACACATAGGTTCTCAGCCCCCCCAAGACTATTTCAAATAAGACTACAATAGCAAGAGCCACTGTAATCACATTCAATGTTGaaa                                      >  1:215866/1‑251 (MQ=255)                                                                                                                                                                                              ggTAAGTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACGACTTGTGCTATGAGCAAACACATAGGTTCTCAGCCCCCCCAAGACTATTTCAAATAAGACTACAATAGCAAGAGCCACTGTAATCACATTCAATGTTGaaa                                      >  1:172304/1‑251 (MQ=255)                                                                                                                                                                                               gTAAGTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACGACTTGTGCTATGAGCAAACACATAGGTTCTCAGCCCCCCCAAGACTATTTCAAATAAGACTACAATAGCAAGAGCCACTGTAATCACATTCAATGTTGaaaa                                     >  1:370690/1‑251 (MQ=255)                                                                                                                                                                                                   gTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACGACTTGTGCTATGAGCAAACACATAGGTtc                                                                                                              <  1:362437/174‑1 (MQ=255)                                                                                                                                                                                                   gTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACGACTTGTGCTATGAGCAAACACATAGGTTCTCAGCCCCCCCAAGac                                                                                              <  1:288021/190‑2 (MQ=255)                                                                                                                                                                                                   gTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACGACTTGTGCTATGAGCAAACACATAGGTTCTCAGCCCCCCCAAGACTATTTCAAATAAGACTACAATAGCaa                                                                    <  1:325042/216‑1 (MQ=255)                                                                                                                                                                                                   gTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACGACTTGTGCTATGAGCAAACACATAGGTTCTCAGCCCCCCCAAGACTATTTCAAATAAGACTACAATAGCAAGAGCCACTGTAATCACATTCAATg                                            <  1:890129/240‑1 (MQ=255)                                                                                                                                                                                                   gTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACGACTTGTGCTATGAGCAAACACATAGGTTCTCAGCCCCCCCAAGACTATTTCAAATAAGACTACAATAGCAAGAGCCACTGTAATCACATTCAATGTTGAAAATCCa                                 >  1:238974/1‑251 (MQ=255)                                                                                                                                                                                                             tGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACGACTTGTGCTATGAGCAAACACATAGGTTCTCAGCCCCCCCAAGACTATTTCAAATAAGACTACAATAGCAAGAGCCACTGTAATCACATTCAATGTTGAAAATCCACGGTGAACCa                       <  1:290346/251‑1 (MQ=255)                                                                                                                                                                                                              gTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACTTCAATACGACTTGTGCTATGAGCAAACACATAGGTTCTCAGCCCCCCCAAGACTATTTCAAATAAGACTACAATAGCAAGAGCCACTGTAATCACATTCAATGt                                           <  1:365951/230‑1 (MQ=255)                                                                                                                                                                                                              gTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACGACTTGtgc                                                                                                                                   <  1:27486/142‑3 (MQ=255)                                                                                                                                                                                                              gTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACGACTTGTGCTATGAGCAAACACATAGGTTCTCAGCCCCCCCAAGACTATTTCAAATAAGACTACAATAg                                                                       <  1:421191/202‑1 (MQ=255)                                                                                                                                                                                                              gTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACGACTTGTGCTATGAGCAAACACATAGGTTCTCAGCCCCCCCAAGACTATTTCAAATAAGACTACAATAGCAAGAGCCACTGTAATCACAt                                                  <  1:402946/223‑1 (MQ=255)                                                                                                                                                                                                              gTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACGACTTGTGCTATGAGCAAACACATAGGTTCTCAGCCCCCCCAAGACTATTTCAAATAAGACTACAATAGCAAGAGCCACTGTAATCACATTCAATGTTGAAAATCCACGGTGAACCaa                      >  1:51621/1‑250 (MQ=255)                                                                                                                                                                                                                       ttCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACGACTTGTGCTATGAGCAAACACATAGGTTCTCAGCCCCCCCAAGACTATTTCAAATAAGACTACAATAGCAAGAGCCACTGTAATCACATTCAATGTTGAAAATCCACGGTGAACCAAGAcc                  >  1:41838/1‑245 (MQ=255)                                                                                                                                                                                                                        tCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACGACTTGTGCTATGAGCAAACACATAGGTTCTCAGCCCCCCCAAGACTATTTCAAGTAAGACTACAATAGCAAGAGCCACTGTAATCACATTCAATGTTGAAAATCCACGGTGAACCAAGACCTTATcc            <  1:596756/251‑1 (MQ=255)                                                                                                                                                                                                                         ccGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACGACTTGTGCTATGAGCAAACACATAGGTTCTCAGCCCCCCCAAGACTATTTCAAATAAGACTACAATAGCAAGAGCCACTGTAATCACATTCAATGTTGAAAATCCACGGTGAACCAAGACCTTATCCa           <  1:250833/251‑1 (MQ=255)                                                                                                                                                                                                                                 gATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGACTTGCTCCCAGTTCGACATCAATACGACTTGTGCTATGAGCAAACACATAGGTTCTCAGCCCCCCCAAGACTATTTCAAATAAGACTACAATAGCAAGAGCCACTGTAATCACATTCAATGTTGAAAATCCACGGTGAACCAAGACCTTATCCATCACGa     >  1:619256/1‑249 (MQ=255)                                                                                                                                                                                                                                  aTCTAATTCACGAACACGAGCCATCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCTAGTTCGACATCAATACGACTTGTGCTATGAGCAAACACATAGGTTCTCAGCCCCCCCAAGACTATTTCAAATAAGACTACAATAGCAAGAGCCACTGTAATCACATTCAATGTTGAAAATCCACGGTGAACCAAGACCTTATCCATCACGACtt  >  1:709014/1‑251 (MQ=255)                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                     AACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGACTAATAAAAACAGACCAAATGACATAACAAGGTAAAGAACCGAGTACAACTAATGTTAATTTTGGGCTGTAATACCACATTACAGCGAAGAAAATAAAAGAGAATAACAAGTCTAAAATAGAGGTAAGTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAACTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAATGACGAAAAAGCCTTGCTCCCAATTCGACATCAATACGACTTGTACTATGAGCAAAAACATAGGTTCTCAAGCCTCCCAAGATTATTTCAAATAAGACTACAATAGCAAGAGCCACTGTAATTACATTCAATGTTGAAAATCCACGGTGAACCAGGACTTTATCCATCACGACTT  >  1/1819954‑1820429

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑