Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,819,876	T→C	intergenic (+37976/+15508)	unknown → / ← unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,819,876	0	T	C	100.0%	219.1 / NA	72	intergenic (+37976/+15508)	unknown/unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/44);  total (28/44)

CAGAAATACCGACCTGTTGGAAATCTTGCCAGATCTGGGCTAAACGAATCACAGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGGTTGATCACCATAACGGCTTTCTGGATCAATTGGATACCTTGCTGTCCGATCGTTGCAAGTACAGTCACTTTAAAACTCACAGCAACATAACTCGCTAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGACTAATAAAAACAGACCAAATGACATAACAAGGTAAAGAACCGAGTACAACTAATGTTAATTTTGGGCTGTAATACCACATTACAGCGAAGAAAATAAA  >  1/1819635‑1820118                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                   cAGAAATACCGACCTGTTGGAAATCTTGCCAGATCTGGGCTAAACGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTa                                                                                                                                                                                                                                           >  1:758095/1‑251 (MQ=255)     aaTACCGACCTGTTGGAAATCTTGCCAGATCTGGGCTAAACGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATccc                                                                                                                                                                                                                                       <  1:762244/251‑1 (MQ=255)      aTACCGACCTGTTGGAAATCTTGCCAGATCTGGGCTAAACGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTTCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATcc                                                                                                                                                                                                                                        <  1:158672/249‑1 (MQ=255)                gTTGGAAATCTTGCCAGATCTGGGCTAAACGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATcc                                                                                                                                                                                                                                        >  1:855756/1‑239 (MQ=255)                  tGGAAATCTTGCCAGATCTGGGCTAAACGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCAt                                                                                                                                                                                                                          >  1:320705/1‑251 (MQ=255)                                     gggCTAAACGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCAt                                                                                                                                                                                                        >  1:88712/1‑250 (MQ=255)                                             cGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAAt                                                                                                                                                                                               <  1:140455/251‑1 (MQ=255)                                             cGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAAt                                                                                                                                                                                               <  1:510748/251‑1 (MQ=255)                                             cGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAAt                                                                                                                                                                                               <  1:42189/251‑1 (MQ=255)                                             cGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAAt                                                                                                                                                                                               <  1:790101/251‑1 (MQ=255)                                               aaTCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTg                                                                                                                                                                                             <  1:804851/251‑1 (MQ=255)                                                      gggCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAAt                                                                                                                                                                                      >  1:631975/1‑251 (MQ=255)                                                         cTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGc                                                                                                                                                                                   <  1:830417/251‑1 (MQ=255)                                                               atTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCAc                                                                                                                                                                             <  1:319263/251‑1 (MQ=255)                                                                               tatTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTgagg                                                                                                                                                                                                                    >  1:40327/1‑196 (MQ=255)                                                                                     aTGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGcc                                                                                                                                                                                                          <  1:8626/200‑1 (MQ=255)                                                                                      tGCAATTAGCTGACCAATGCTTGAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGc                                                                                                                                                                                                           <  1:485130/198‑1 (MQ=255)                                                                                       gCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGAtt                                                                                                                                                     >  1:356995/1‑251 (MQ=255)                                                                                               cTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGc                                                                                                                                               <  1:842801/249‑1 (MQ=255)                                                                                                   ccAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAAt                                                                                                                                                                                               <  1:160750/197‑1 (MQ=255)                                                                                                      aTACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTg                                                                                                                                       >  1:514761/1‑250 (MQ=255)                                                                                                        aCTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGAc                                                                                                                                                          <  1:172235/229‑1 (MQ=255)                                                                                                        aCTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGa                                                                                                                                    >  1:188431/1‑251 (MQ=255)                                                                                                        aCTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGa                                                                                                                                    >  1:744846/1‑251 (MQ=255)                                                                                                        aCTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGa                                                                                                                                    >  1:569366/1‑251 (MQ=255)                                                                                                                      aaaTAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGc                                                                                                                      <  1:18592/251‑1 (MQ=255)                                                                                                                         tAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGt                                                                                                                   >  1:148509/1‑251 (MQ=255)                                                                                                                          aaCTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTGAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGt                                                                                                                   >  1:184848/1‑250 (MQ=255)                                                                                                                             tAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTcc                                                                                                               <  1:629131/251‑1 (MQ=255)                                                                                                                                     cgcCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAAt                                                                                                        <  1:563140/250‑1 (MQ=255)                                                                                                                                     cgcCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAAt                                                                                                        <  1:6594/250‑1 (MQ=255)                                                                                                                                     cgcCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAAt                                                                                                        <  1:50691/250‑1 (MQ=255)                                                                                                                                         aaGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGAc                                                                                                   <  1:899703/251‑1 (MQ=255)                                                                                                                                         aaGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGAc                                                                                                   <  1:494647/251‑1 (MQ=255)                                                                                                                                         aaGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGAc                                                                                                   <  1:759560/251‑1 (MQ=255)                                                                                                                                           gCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGcc                                                                                                                                                                                                          <  1:897809/146‑1 (MQ=255)                                                                                                                                           gCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTatcatc                                                                                                                          <  1:395511/226‑1 (MQ=255)                                                                                                                                           gCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAAt                                                                                                        <  1:160408/244‑1 (MQ=255)                                                                                                                                           gCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGACta                                                                                                 >  1:485025/1‑251 (MQ=255)                                                                                                                                                agattGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTcaacaa                                                                                                                                                                  >  1:405157/4‑181 (MQ=255)                                                                                                                                                   ttGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGACTAATAAAAAc                                                                                         <  1:351494/251‑1 (MQ=255)                                                                                                                                                        cACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGACTAATAAAAAc                                                                                         <  1:95563/246‑1 (MQ=255)                                                                                                                                                        cACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGACTAATAAAAACAGAc                                                                                     >  1:134940/1‑250 (MQ=255)                                                                                                                                                          ccATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAAt                                                                                                        >  1:495826/1‑229 (MQ=255)                                                                                                                                                          ccATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGACtaata                                                                                              >  1:816399/1‑239 (MQ=255)                                                                                                                                                          ccATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGACTAATAAAAAc                                                                                         <  1:451660/244‑1 (MQ=255)                                                                                                                                                          ccATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGACTAATAAAAACAGACCaa                                                                                  >  1:343116/1‑251 (MQ=255)                                                                                                                                                                 ggCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGACTAATAAAAAc                                                                                         >  1:268936/1‑237 (MQ=255)                                                                                                                                                                    tttCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGACTAATAAAAACAGACCAAATGACATAAc                                                                        <  1:325811/251‑1 (MQ=255)                                                                                                                                                                               gttGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGACtaata                                                                                              <  1:195721/217‑1 (MQ=255)                                                                                                                                                                                ttGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGACTAATAAAAACAGACCAAATGACATAACAAGGTAAAGAAc                                                            <  1:385120/251‑1 (MQ=255)                                                                                                                                                                                ttGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTAATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGACTAATAAAAACAGACCAAATGACATAACAAGGTAAAGAAc                                                            <  1:17494/251‑1 (MQ=255)                                                                                                                                                                                       ccTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGACTAATAAAAACAGACCAAATGACATAACaa                                                                      <  1:25969/234‑1 (MQ=255)                                                                                                                                                                                       ccTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGACTAATAAAAACAGACCAAATGACATAACAAGGTAAAGAAc                                                            <  1:711694/244‑1 (MQ=255)                                                                                                                                                                                       ccTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGACTAATAAAAACAGACCAAATGACATAACAAGGTAAAGAACCGAGTAc                                                     <  1:368690/251‑1 (MQ=255)                                                                                                                                                                                          tgctgcCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGACTAATAAAAACAGACCAAATGACATAACAAGGTAAAGAACCGAGTACAAc                                                  <  1:322348/251‑1 (MQ=255)                                                                                                                                                                                              gcccGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAAt                                                                                                        >  1:234815/3‑193 (MQ=255)                                                                                                                                                                                              gcccGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGACTAATAAAAACAGACCAAATGACATAACAAGGTAAAGAACCGAGTACAACTAAt                                              >  1:783756/3‑251 (MQ=255)                                                                                                                                                                                                       ttGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGACTAATAAAAACAGACCAAATGACATAACAAGGTAAAGAACCGAGTACAAc                                                  <  1:305816/238‑1 (MQ=255)                                                                                                                                                                                                              cacggTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGACTAATAAAAACAGACCAAATGACATAACAAGGTAAAGAAc                                                            >  1:742774/5‑221 (MQ=255)                                                                                                                                                                                                                     aCTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGACTAATAAAAACAGACCAAATGACATAACAAGGTAAAGAACCGAGTACAACTAATGTTAATTTTGGGCTGTAATACca                       <  1:751109/251‑1 (MQ=255)                                                                                                                                                                                                                      cTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTga                                                                                                                                                                                                                      >  1:562578/1‑59 (MQ=255)                                                                                                                                                                                                                      cTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATg                                                                                                                                                                                     <  1:504220/92‑1 (MQ=255)                                                                                                                                                                                                                      cTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGACTAATAAAAACAGACCAAATGACATAACAAGGTAAAGAACCGAGTACAACTAATGTTAATTTTGGGCTGTAATACcac                      <  1:270933/251‑1 (MQ=255)                                                                                                                                                                                                                      cTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGACTAATAAAAACAGACCAAATGACATAACAAGGTAAAGAACCGAGTACAACTAATGTTAATTTTGGGCTGTAATACcac                      <  1:89053/251‑1 (MQ=255)                                                                                                                                                                                                                           aaaCTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGACTAATAAAAACAGACCAAATGACATAACAAGGTAAAGAACCGAGTACAACTAATGTTAATTTTGGGCTGTAATACCACAtaac                 <  1:655645/251‑4 (MQ=255)                                                                                                                                                                                                                             aCTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGACTAATAAAAACAGACCAAATGACATAACAAGGTAAAGAACCGAGTACAACTAATGTTAATTTTGGGCTGTAATACCACAtaacgg               <  1:858477/251‑6 (MQ=255)                                                                                                                                                                                                                                  caGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGACTAATAAAGACAGACCAAATGACATAACAAGGTAAAGAACCGAGTACAACTAATg                                             >  1:455139/1‑216 (MQ=255)                                                                                                                                                                                                                                     cAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGACTAATAAAAAc                                                                                         <  1:301377/169‑1 (MQ=255)                                                                                                                                                                                                                                     cAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGACTAATAAAAACAGACCAAATGACATAACAAGGTAAAGAACCGAGTACAACTAATGTTAATTTTGGGCTGTAATACCACATAACGGCGAAGaaa       >  1:54537/1‑251 (MQ=255)                                                                                                                                                                                                                                             cTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGACTAATAAAAACAGACCAAATGACATAACAAGGTAAAGAACCGAGTACAACTAATGTTAAttt                                      >  1:537527/1‑212 (MQ=255)                                                                                                                                                                                                                                             cTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGACTAATAAAAACAGACCAAATGACATAACAAGGTAAAGAACCGAGTACAACTAATGTTAATTTTGGGCTGTAATACCACATAACGGCGAAGAAAATaaa  >  1:243717/1‑248 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                   CAGAAATACCGACCTGTTGGAAATCTTGCCAGATCTGGGCTAAACGAATCACAGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGGTTGATCACCATAACGGCTTTCTGGATCAATTGGATACCTTGCTGTCCGATCGTTGCAAGTACAGTCACTTTAAAACTCACAGCAACATAACTCGCTAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGACTAATAAAAACAGACCAAATGACATAACAAGGTAAAGAACCGAGTACAACTAATGTTAATTTTGGGCTGTAATACCACATTACAGCGAAGAAAATAAA  >  1/1819635‑1820118

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑