Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,819,859	A→G	intergenic (+37959/+15525)	unknown → / ← unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,819,859	0	A	G	100.0%	224.3 / NA	76	intergenic (+37959/+15525)	unknown/unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (30/46);  total (30/46)

AAGCACATCACCCAAACGAGTAACAGAAATACCGACCTGTTGGAAATCTTGCCAGATCTGGGCTAAACGAATCACAGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGGTTGATCACCATAACGGCTTTCTGGATCAATTGGATACCTTGCTGTCCGATCGTTGCAAGTACAGTCACTTTAAAACTCACAGCAACATAACTCGCTAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGACTAATAAAAACAGACCAAATGACATAACAAGGTAAAGAACCGAGTACAACTAATGTTAATTTTGGGCTGTAATACCACATTACAG  >  1/1819612‑1820105                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                       aaGCACATCACCTAAGCGAGTAACAGAAATACCGACCTGTTGGAAATCTTGCCAGATCTGGGCTAAACGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAg                                                                                                                                                                                                                                                     >  1:405483/1‑251 (MQ=255)      caTCACCTAAGCGAGTAACAGAAATACCGACCTGTTGGAAATCTTGCCAGATCTGGGCTAAACGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACa                                                                                                                                                                                                                                                >  1:668156/1‑251 (MQ=255)       aTCACCTAAGCGAGTAACAGAAATACCGACCTGTTGGAAATCTTGCCAGATCTGGGCTAAACGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACAt                                                                                                                                                                                                                                               <  1:7766/251‑1 (MQ=255)         cacctaagcGAGTAACAGAAATACCGACCTGTTGGAAATCTTGCCAGATCTGGGCTAAACGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATaa                                                                                                                                                                                                                                             >  1:546045/9‑251 (MQ=255)          acctaagcGAGTAACAGAAATACCGACCTGTTGGAAATCTTGCCAGATCTGGGCTAAACGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATaa                                                                                                                                                                                                                                             >  1:120985/8‑250 (MQ=255)                gcGAGTAACAGAAATACCGACCTGTTGGAAATCTTGCCAGATCTGGGCTAAACGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGcca                                                                                                                                                                                                                                      >  1:14781/2‑249 (MQ=255)                        cAGAAATACCGACCTGTTGGAAATCTTGCCAGATCTGGGCTAAACGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTa                                                                                                                                                                                                                              >  1:758095/1‑251 (MQ=255)                            aaTACCGACCTGTTGGAAATCTTGCCAGATCTGGGCTAAACGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATccc                                                                                                                                                                                                                          <  1:762244/251‑1 (MQ=255)                             aTACCGACCTGTTGGAAATCTTGCCAGATCTGGGCTAAACGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTTCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATcc                                                                                                                                                                                                                           <  1:158672/249‑1 (MQ=255)                                       gTTGGAAATCTTGCCAGATCTGGGCTAAACGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATcc                                                                                                                                                                                                                           >  1:855756/1‑239 (MQ=255)                                         tGGAAATCTTGCCAGATCTGGGCTAAACGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCAt                                                                                                                                                                                                             >  1:320705/1‑251 (MQ=255)                                                            gggCTAAACGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACAt                                                                                                                                                                                                                                               <  1:895655/198‑1 (MQ=255)                                                            gggCTAAACGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCAt                                                                                                                                                                                           >  1:88712/1‑250 (MQ=255)                                                                    cGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAAt                                                                                                                                                                                  <  1:790101/251‑1 (MQ=255)                                                                    cGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAAt                                                                                                                                                                                  <  1:510748/251‑1 (MQ=255)                                                                    cGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAAt                                                                                                                                                                                  <  1:42189/251‑1 (MQ=255)                                                                    cGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAAt                                                                                                                                                                                  <  1:140455/251‑1 (MQ=255)                                                                      aaTCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTg                                                                                                                                                                                <  1:804851/251‑1 (MQ=255)                                                                             gggCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAAt                                                                                                                                                                         >  1:631975/1‑251 (MQ=255)                                                                                cTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGc                                                                                                                                                                      <  1:830417/251‑1 (MQ=255)                                                                                      atTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCAc                                                                                                                                                                <  1:319263/251‑1 (MQ=255)                                                                                                      tatTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTgagg                                                                                                                                                                                                       >  1:40327/1‑196 (MQ=255)                                                                                                            aTGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGcc                                                                                                                                                                                             <  1:8626/200‑1 (MQ=255)                                                                                                             tGCAATTAGCTGACCAATGCTTGAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGc                                                                                                                                                                                              <  1:485130/198‑1 (MQ=255)                                                                                                              gCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGAtt                                                                                                                                        >  1:356995/1‑251 (MQ=255)                                                                                                                      cTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGc                                                                                                                                  <  1:842801/249‑1 (MQ=255)                                                                                                                        gACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAAc                                                                                                                                                                                                                                            >  1:388129/1‑141 (MQ=255)                                                                                                                          ccAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAAt                                                                                                                                                                                  <  1:160750/197‑1 (MQ=255)                                                                                                                             aTACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTg                                                                                                                          >  1:514761/1‑250 (MQ=255)                                                                                                                               aCTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGAc                                                                                                                                             <  1:172235/229‑1 (MQ=255)                                                                                                                               aCTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGa                                                                                                                       >  1:744846/1‑251 (MQ=255)                                                                                                                               aCTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGa                                                                                                                       >  1:188431/1‑251 (MQ=255)                                                                                                                               aCTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGa                                                                                                                       >  1:569366/1‑251 (MQ=255)                                                                                                                                             aaaTAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGc                                                                                                         <  1:18592/251‑1 (MQ=255)                                                                                                                                                tAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGt                                                                                                      >  1:148509/1‑251 (MQ=255)                                                                                                                                                 aaCTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTGAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGt                                                                                                      >  1:184848/1‑250 (MQ=255)                                                                                                                                                    tAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTcc                                                                                                  <  1:629131/251‑1 (MQ=255)                                                                                                                                                            cgcCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAAt                                                                                           <  1:6594/250‑1 (MQ=255)                                                                                                                                                            cgcCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAAt                                                                                           <  1:563140/250‑1 (MQ=255)                                                                                                                                                            cgcCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAAt                                                                                           <  1:50691/250‑1 (MQ=255)                                                                                                                                                                aaGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGAc                                                                                      <  1:759560/251‑1 (MQ=255)                                                                                                                                                                aaGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGAc                                                                                      <  1:494647/251‑1 (MQ=255)                                                                                                                                                                aaGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGAc                                                                                      <  1:899703/251‑1 (MQ=255)                                                                                                                                                                  gCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGc                                                                                                                                                                                                                                                    <  1:552145/91‑1 (MQ=255)                                                                                                                                                                  gCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGcc                                                                                                                                                                                             <  1:897809/146‑1 (MQ=255)                                                                                                                                                                  gCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTatcatc                                                                                                             <  1:395511/226‑1 (MQ=255)                                                                                                                                                                  gCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAAt                                                                                           <  1:160408/244‑1 (MQ=255)                                                                                                                                                                  gCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGACta                                                                                    >  1:485025/1‑251 (MQ=255)                                                                                                                                                                       agattGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTcaacaa                                                                                                                                                     >  1:405157/4‑181 (MQ=255)                                                                                                                                                                          ttGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGACTAATAAAAAc                                                                            <  1:351494/251‑1 (MQ=255)                                                                                                                                                                               cACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGACTAATAAAAAc                                                                            <  1:95563/246‑1 (MQ=255)                                                                                                                                                                               cACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGACTAATAAAAACAGAc                                                                        >  1:134940/1‑250 (MQ=255)                                                                                                                                                                                 ccATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAAt                                                                                           >  1:495826/1‑229 (MQ=255)                                                                                                                                                                                 ccATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGACtaata                                                                                 >  1:816399/1‑239 (MQ=255)                                                                                                                                                                                 ccATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGACTAATAAAAAc                                                                            <  1:451660/244‑1 (MQ=255)                                                                                                                                                                                 ccATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGACTAATAAAAACAGACCaa                                                                     >  1:343116/1‑251 (MQ=255)                                                                                                                                                                                        ggCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGACTAATAAAAAc                                                                            >  1:268936/1‑237 (MQ=255)                                                                                                                                                                                           tttCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGACTAATAAAAACAGACCAAATGACATAAc                                                           <  1:325811/251‑1 (MQ=255)                                                                                                                                                                                                      gttGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGACtaata                                                                                 <  1:195721/217‑1 (MQ=255)                                                                                                                                                                                                       ttGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGACTAATAAAAACAGACCAAATGACATAACAAGGTAAAGAAc                                               <  1:385120/251‑1 (MQ=255)                                                                                                                                                                                                       ttGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTAATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGACTAATAAAAACAGACCAAATGACATAACAAGGTAAAGAAc                                               <  1:17494/251‑1 (MQ=255)                                                                                                                                                                                                              ccTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGACTAATAAAAACAGACCAAATGACATAACaa                                                         <  1:25969/234‑1 (MQ=255)                                                                                                                                                                                                              ccTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGACTAATAAAAACAGACCAAATGACATAACAAGGTAAAGAAc                                               <  1:711694/244‑1 (MQ=255)                                                                                                                                                                                                              ccTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGACTAATAAAAACAGACCAAATGACATAACAAGGTAAAGAACCGAGTAc                                        <  1:368690/251‑1 (MQ=255)                                                                                                                                                                                                                 tgctgcCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGACTAATAAAAACAGACCAAATGACATAACAAGGTAAAGAACCGAGTACAAc                                     <  1:322348/251‑1 (MQ=255)                                                                                                                                                                                                                     gcccGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAAt                                                                                           >  1:234815/3‑193 (MQ=255)                                                                                                                                                                                                                     gcccGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGACTAATAAAAACAGACCAAATGACATAACAAGGTAAAGAACCGAGTACAACTAAt                                 >  1:783756/3‑251 (MQ=255)                                                                                                                                                                                                                              ttGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGACTAATAAAAACAGACCAAATGACATAACAAGGTAAAGAACCGAGTACAAc                                     <  1:305816/238‑1 (MQ=255)                                                                                                                                                                                                                                     cacggTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGACTAATAAAAACAGACCAAATGACATAACAAGGTAAAGAAc                                               >  1:742774/5‑221 (MQ=255)                                                                                                                                                                                                                                            aCTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGACTAATAAAAACAGACCAAATGACATAACAAGGTAAAGAACCGAGTACAACTAATGTTAATTTTGGGCTGTAATACca          <  1:751109/251‑1 (MQ=255)                                                                                                                                                                                                                                             cTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTga                                                                                                                                                                                                         >  1:562578/1‑59 (MQ=255)                                                                                                                                                                                                                                             cTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATg                                                                                                                                                                        <  1:504220/92‑1 (MQ=255)                                                                                                                                                                                                                                             cTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGACTAATAAAAACAGACCAAATGACATAACAAGGTAAAGAACCGAGTACAACTAATGTTAATTTTGGGCTGTAATACcac         <  1:270933/251‑1 (MQ=255)                                                                                                                                                                                                                                             cTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGACTAATAAAAACAGACCAAATGACATAACAAGGTAAAGAACCGAGTACAACTAATGTTAATTTTGGGCTGTAATACcac         <  1:89053/251‑1 (MQ=255)                                                                                                                                                                                                                                                  aaaCTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGACTAATAAAAACAGACCAAATGACATAACAAGGTAAAGAACCGAGTACAACTAATGTTAATTTTGGGCTGTAATACCACAtaac    <  1:655645/251‑4 (MQ=255)                                                                                                                                                                                                                                                    aCTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGACTAATAAAAACAGACCAAATGACATAACAAGGTAAAGAACCGAGTACAACTAATGTTAATTTTGGGCTGTAATACCACAtaacgg  <  1:858477/251‑6 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                       AAGCACATCACCCAAACGAGTAACAGAAATACCGACCTGTTGGAAATCTTGCCAGATCTGGGCTAAACGAATCACAGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGGTTGATCACCATAACGGCTTTCTGGATCAATTGGATACCTTGCTGTCCGATCGTTGCAAGTACAGTCACTTTAAAACTCACAGCAACATAACTCGCTAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGACTAATAAAAACAGACCAAATGACATAACAAGGTAAAGAACCGAGTACAACTAATGTTAATTTTGGGCTGTAATACCACATTACAG  >  1/1819612‑1820105

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑