Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,819,825	T→C	intergenic (+37925/+15559)	unknown → / ← unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,819,825	0	T	C	100.0%	217.5 / NA	76	intergenic (+37925/+15559)	unknown/unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (33/43);  total (33/43)

GTATTATTCTCTGTAGGCGAATTAAGCACATCACCCAAACGAGTAACAGAAATACCGACCTGTTGGAAATCTTGCCAGATCTGGGCTAAACGAATCACAGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGGTTGATCACCATAACGGCTTTCTGGATCAATTGGATACCTTGCTGTCCGATCGTTGCAAGTACAGTCACTTTAAAACTCACAGCAACATAACTCGCTAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGACTAATAAAAACAGACCAAATGACATAACAAGGTAAAGAACCGAGTACAAC  >  1/1819589‑1820070                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                      gTATTATTCTCTGTAGGCGAATTAAGCACATCACCTAAGCGAGTAACAGAAATACCGACCTGTTGGAAATCTTGCCAGATCTGGGCTAAACGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAg                                                                                                                                                                                                                                         >  1:111704/1‑251 (MQ=255) gTATTATTCTCTGTAGGCGAATTAAGCACATCACCTAAGCGAGTAACAGAAATACCGACCTGTTGGAAATCTTGCCAGATCTGGGCTAAACGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAg                                                                                                                                                                                                                                         >  1:544168/1‑251 (MQ=255)   attattCTCTGTAGGCGAATTAAGCACATCACCTAAGCGAGTAACAGAAATACCGACCTGTTGGAAATCTTGCCAGATCTGGGCTAAACGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAgca                                                                                                                                                                                                                                       <  1:801320/251‑3 (MQ=255)         ctctGTAGGCGAATTAAGCACATCACCTAAGCGAGTAACAGAAATACCGACCTGTTGGAAATCTTGCCAGATCTGGGCTAAACGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCa                                                                                                                                                                                                                                 >  1:150534/1‑251 (MQ=255)          tctGTAGGCGAATTAAGCACATCACCTAAGCGAGTAACAGAAATACCGACCTGTTGGAAATCTTGCCAGATCTGGGCTAAACGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCAc                                                                                                                                                                                                                                <  1:741488/251‑1 (MQ=255)           ctGTAGGCGAATTAAGCACATCACCTAAGCGAGTAACAGAAATACCGACCTGTTGGAAATCTTGCCAGATCTGGGCTAAACGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCa                                                                                                                                                                                                                                 >  1:375073/1‑249 (MQ=255)                  cGAATTAAGCACATCACCTAAGCGAGTAACAGAAATACCGACCTGTTGGAAATCTTGCCAGATCTGGGCTAAACGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAAc                                                                                                                                                                                                                        <  1:475574/251‑1 (MQ=255)                   gAATTAAGCACATCACCTAAGCGAGTAACAGAAATACCGACCTGTTGGAAATCTTGCCAGATCTGGGCTAAACGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTaaaa                                                                                                                                                                                                                         >  1:197262/1‑249 (MQ=255)                        aaGCACATCACCTAAGCGAGTAACAGAAATACCGACCTGTTGGAAATCTTGCCAGATCTGGGCTAAACGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAg                                                                                                                                                                                                                  >  1:405483/1‑251 (MQ=255)                             caTCACCTAAGCGAGTAACAGAAATACCGACCTGTTGGAAATCTTGCCAGATCTGGGCTAAACGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACa                                                                                                                                                                                                             >  1:668156/1‑251 (MQ=255)                              aTCACCTAAGCGAGTAACAGAAATACCGACCTGTTGGAAATCTTGCCAGATCTGGGCTAAACGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACAt                                                                                                                                                                                                            <  1:7766/251‑1 (MQ=255)                                cacctaagcGAGTAACAGAAATACCGACCTGTTGGAAATCTTGCCAGATCTGGGCTAAACGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATaa                                                                                                                                                                                                          >  1:546045/9‑251 (MQ=255)                                 acctaagcGAGTAACAGAAATACCGACCTGTTGGAAATCTTGCCAGATCTGGGCTAAACGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAgca                                                                                                                                                                                                                                       <  1:168122/214‑3 (MQ=255)                                 acctaagcGAGTAACAGAAATACCGACCTGTTGGAAATCTTGCCAGATCTGGGCTAAACGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATaa                                                                                                                                                                                                          >  1:120985/8‑250 (MQ=255)                                       gcGAGTAACAGAAATACCGACCTGTTGGAAATCTTGCCAGATCTGGGCTAAACGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGcca                                                                                                                                                                                                   >  1:14781/2‑249 (MQ=255)                                              aCAGAAATACCGACCTGTTGGAAATCTTGCCAGATCTGGGCTAAACGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAAc                                                                                                                                                                                                                        >  1:490144/1‑223 (MQ=255)                                               cAGAAATACCGACCTGTTGGAAATCTTGCCAGATCTGGGCTAAACGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTa                                                                                                                                                                                           >  1:758095/1‑251 (MQ=255)                                                   aaTACCGACCTGTTGGAAATCTTGCCAGATCTGGGCTAAACGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATccc                                                                                                                                                                                       <  1:762244/251‑1 (MQ=255)                                                    aTACCGACCTGTTGGAAATCTTGCCAGATCTGGGCTAAACGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTTCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATcc                                                                                                                                                                                        <  1:158672/249‑1 (MQ=255)                                                              gTTGGAAATCTTGCCAGATCTGGGCTAAACGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATcc                                                                                                                                                                                        >  1:855756/1‑239 (MQ=255)                                                                tGGAAATCTTGCCAGATCTGGGCTAAACGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCAt                                                                                                                                                                          >  1:320705/1‑251 (MQ=255)                                                                                   gggCTAAACGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACAt                                                                                                                                                                                                            <  1:895655/198‑1 (MQ=255)                                                                                   gggCTAAACGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCAt                                                                                                                                                        >  1:88712/1‑250 (MQ=255)                                                                                           cGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAAt                                                                                                                                               <  1:790101/251‑1 (MQ=255)                                                                                           cGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAAt                                                                                                                                               <  1:510748/251‑1 (MQ=255)                                                                                           cGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAAt                                                                                                                                               <  1:42189/251‑1 (MQ=255)                                                                                           cGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAAt                                                                                                                                               <  1:140455/251‑1 (MQ=255)                                                                                             aaTCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTg                                                                                                                                             <  1:804851/251‑1 (MQ=255)                                                                                                    gggCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAAt                                                                                                                                      >  1:631975/1‑251 (MQ=255)                                                                                                       cTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGc                                                                                                                                   <  1:830417/251‑1 (MQ=255)                                                                                                             atTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCAc                                                                                                                             <  1:319263/251‑1 (MQ=255)                                                                                                                         aGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACttt                                                                                                                                                                                                                             >  1:281240/1‑143 (MQ=255)                                                                                                                             tatTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTgagg                                                                                                                                                                    >  1:40327/1‑196 (MQ=255)                                                                                                                                   aTGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGcc                                                                                                                                                          <  1:8626/200‑1 (MQ=255)                                                                                                                                    tGCAATTAGCTGACCAATGCTTGAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGc                                                                                                                                                           <  1:485130/198‑1 (MQ=255)                                                                                                                                     gCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAgca                                                                                                                                                                                                                                       <  1:306740/121‑3 (MQ=255)                                                                                                                                     gCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGAtt                                                                                                     >  1:356995/1‑251 (MQ=255)                                                                                                                                             cTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGc                                                                                               <  1:842801/249‑1 (MQ=255)                                                                                                                                               gACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAAc                                                                                                                                                                                                         >  1:388129/1‑141 (MQ=255)                                                                                                                                                 ccAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAAt                                                                                                                                               <  1:160750/197‑1 (MQ=255)                                                                                                                                                    aTACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTg                                                                                       >  1:514761/1‑250 (MQ=255)                                                                                                                                                      aCTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGAc                                                                                                          <  1:172235/229‑1 (MQ=255)                                                                                                                                                      aCTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGa                                                                                    >  1:569366/1‑251 (MQ=255)                                                                                                                                                      aCTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGa                                                                                    >  1:744846/1‑251 (MQ=255)                                                                                                                                                      aCTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGa                                                                                    >  1:188431/1‑251 (MQ=255)                                                                                                                                                                    aaaTAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGc                                                                      <  1:18592/251‑1 (MQ=255)                                                                                                                                                                       tAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGt                                                                   >  1:148509/1‑251 (MQ=255)                                                                                                                                                                        aaCTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTGAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGt                                                                   >  1:184848/1‑250 (MQ=255)                                                                                                                                                                           tAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTcc                                                               <  1:629131/251‑1 (MQ=255)                                                                                                                                                                                   cgcCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAAt                                                        <  1:50691/250‑1 (MQ=255)                                                                                                                                                                                   cgcCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAAt                                                        <  1:563140/250‑1 (MQ=255)                                                                                                                                                                                   cgcCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAAt                                                        <  1:6594/250‑1 (MQ=255)                                                                                                                                                                                       aaGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGAc                                                   <  1:494647/251‑1 (MQ=255)                                                                                                                                                                                       aaGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGAc                                                   <  1:899703/251‑1 (MQ=255)                                                                                                                                                                                       aaGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGAc                                                   <  1:759560/251‑1 (MQ=255)                                                                                                                                                                                         gCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGc                                                                                                                                                                                                                 <  1:552145/91‑1 (MQ=255)                                                                                                                                                                                         gCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGcc                                                                                                                                                          <  1:897809/146‑1 (MQ=255)                                                                                                                                                                                         gCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTatcatc                                                                          <  1:395511/226‑1 (MQ=255)                                                                                                                                                                                         gCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAAt                                                        <  1:160408/244‑1 (MQ=255)                                                                                                                                                                                         gCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGACta                                                 >  1:485025/1‑251 (MQ=255)                                                                                                                                                                                              agattGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTcaacaa                                                                                                                  >  1:405157/4‑181 (MQ=255)                                                                                                                                                                                                 ttGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGACTAATAAAAAc                                         <  1:351494/251‑1 (MQ=255)                                                                                                                                                                                                      cACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGACTAATAAAAAc                                         <  1:95563/246‑1 (MQ=255)                                                                                                                                                                                                      cACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGACTAATAAAAACAGAc                                     >  1:134940/1‑250 (MQ=255)                                                                                                                                                                                                        ccATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAAt                                                        >  1:495826/1‑229 (MQ=255)                                                                                                                                                                                                        ccATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGACtaata                                              >  1:816399/1‑239 (MQ=255)                                                                                                                                                                                                        ccATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGACTAATAAAAAc                                         <  1:451660/244‑1 (MQ=255)                                                                                                                                                                                                        ccATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGACTAATAAAAACAGACCaa                                  >  1:343116/1‑251 (MQ=255)                                                                                                                                                                                                               ggCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGACTAATAAAAAc                                         >  1:268936/1‑237 (MQ=255)                                                                                                                                                                                                                  tttCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGACTAATAAAAACAGACCAAATGACATAAc                        <  1:325811/251‑1 (MQ=255)                                                                                                                                                                                                                             gttGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGACtaata                                              <  1:195721/217‑1 (MQ=255)                                                                                                                                                                                                                              ttGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGACTAATAAAAACAGACCAAATGACATAACAAGGTAAAGAAc            <  1:385120/251‑1 (MQ=255)                                                                                                                                                                                                                              ttGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTAATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGACTAATAAAAACAGACCAAATGACATAACAAGGTAAAGAAc            <  1:17494/251‑1 (MQ=255)                                                                                                                                                                                                                                     ccTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGACTAATAAAAACAGACCAAATGACATAACaa                      <  1:25969/234‑1 (MQ=255)                                                                                                                                                                                                                                     ccTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGACTAATAAAAACAGACCAAATGACATAACAAGGTAAAGAAc            <  1:711694/244‑1 (MQ=255)                                                                                                                                                                                                                                     ccTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGACTAATAAAAACAGACCAAATGACATAACAAGGTAAAGAACCGAGTAc     <  1:368690/251‑1 (MQ=255)                                                                                                                                                                                                                                        tgctgcCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGACTAATAAAAACAGACCAAATGACATAACAAGGTAAAGAACCGAGTACAAc  <  1:322348/251‑1 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                      GTATTATTCTCTGTAGGCGAATTAAGCACATCACCCAAACGAGTAACAGAAATACCGACCTGTTGGAAATCTTGCCAGATCTGGGCTAAACGAATCACAGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGGTTGATCACCATAACGGCTTTCTGGATCAATTGGATACCTTGCTGTCCGATCGTTGCAAGTACAGTCACTTTAAAACTCACAGCAACATAACTCGCTAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGACTAATAAAAACAGACCAAATGACATAACAAGGTAAAGAACCGAGTACAAC  >  1/1819589‑1820070

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑