Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,819,496	T→C	intergenic (+37596/+15888)	unknown → / ← unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,819,496	0	T	C	100.0%	205.6 / NA	67	intergenic (+37596/+15888)	unknown/unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (35/32);  total (35/32)

GGATTGGTTAACGCAATATTCTCTCTGATACTTCGATTAAGCAACACATTATCTTGCAAGACGACACCGACTTGCCGGCGCAACCAGTTTGGATCGGCTAGCGCCAAATCGTGACCGTCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATGAGTTTAGTAAGCGTACTTTTCCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATATTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGGATTTCCGGTAATGATACTGAGGCGGTATTATTCTCTGTAGGCGAATTAAGCACATCACCCAAACGAGTAACAGAAATACCGACCTGTTGGAAATCTTGCCAGATCTGGGCTAAACGAATCACAGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATAC  >  1/1819253‑1819739                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                    ggATTGGTTAACGCAATATTCTCTCTGATACTTCGATTAAGCAACACATTATCTTGCAGGACGACACCTACTTGCCGGCGTAACCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTaa                                                                                                                                                                                                                                              >  1:573076/1‑251 (MQ=255)           aCGCAATATTCTCTCTGATACTTCGATTAAGCAACACATTATCTTGCAGGACGACACCTACTTGCCGGCGTAACCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAggg                                                                                                                                                                                                                                    >  1:146314/1‑251 (MQ=255)            cGCAATATTCTCTCTGATACTTCGATTAAGCAACACATTATCTTGCAGGACGACACCTACTTGCCGGCGTAACCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAgggg                                                                                                                                                                                                                                   <  1:380542/251‑1 (MQ=255)                   ttCTCTCTGATACTTCGATTAAGCAACACATTATCTTGCAGGACGACACCTACTTGCCGGCGTAACCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCgg                                                                                                                                                                                                                            <  1:111098/251‑1 (MQ=255)                   ttCTCTCTGATACTTCGATTAAGCAACACATTATCTTGCAGGACGACACCTACTTGCCGGCGTAACCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCgg                                                                                                                                                                                                                            <  1:244782/251‑1 (MQ=255)                       ctctGATACTTCGATTAAGCAACACATTATCTTGCAGGACGACACCTACTTGCCGGCGTAACCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTta                                                                                                                                                                                                                        >  1:198995/1‑251 (MQ=255)                                            acacATTATCTTGCAGGACGACACCTACTTGCCGGCGTAACCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTa                                                                                                                                                                                                   >  1:129552/1‑251 (MQ=255)                                                 ttATCTTGCAGGACGACACCTACTTGCCGGCGTAACCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTaaaa                                                                                                                                                                                                >  1:414736/1‑249 (MQ=255)                                                   aTCTTGCAGGACGACACCTACTTGCCGGCGTAACCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTaa                                                                                                                                                                                            >  1:814257/1‑251 (MQ=255)                                                           ggacgacACCTACTTGCCGGCGTAACCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCAcc                                                                                                                                                                                     >  1:647313/2‑250 (MQ=255)                                                             acgacACCTACTTGCCGGCGTAACCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAgggg                                                                                                                                                                                                                                   >  1:183568/1‑202 (MQ=255)                                                                               cgtaaCCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGAATTTCCGGTAATGAt                                                                                                                                                                  >  1:756227/4‑249 (MQ=255)                                                                                 taaCCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGAATTTCCGGTAATGATACTg                                                                                                                                                              <  1:387956/250‑1 (MQ=255)                                                                                  aaCCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAAtctc                                                                                                                                                                                        <  1:745239/224‑1 (MQ=255)                                                                                  aaCCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGAATTTCCGGTAATGATACTGa                                                                                                                                                             >  1:636046/1‑251 (MQ=255)                                                                                      aGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGAATTTCCGGTAATGATACTGAGGCg                                                                                                                                                         >  1:735619/1‑251 (MQ=255)                                                                                       gTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGAATTTCCGGTAATGATACTGAGGCgg                                                                                                                                                        >  1:684688/1‑251 (MQ=255)                                                                                       gTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGAATTTCCGGTAATGATACTGAGGCgg                                                                                                                                                        >  1:540391/1‑251 (MQ=255)                                                                                             aTCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGAATTTCCGGTAATGATACTGAGGCGGtattat                                                                                                                                                  <  1:560237/251‑1 (MQ=255)                                                                                               cGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGAATTTCCGGTAATGATACTGAGGCGGTATTATtc                                                                                                                                                <  1:534675/251‑1 (MQ=255)                                                                                               cGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGAATTTCCGGTAATGATACTGAGGCGGTATTATtc                                                                                                                                                <  1:61694/251‑1 (MQ=255)                                                                                               cGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGAATTTCCGGTAATGATACTGAGGCGGTATTATtc                                                                                                                                                <  1:247570/251‑1 (MQ=255)                                                                                               cGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATACCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGAATTTCCGGTAATGATACTGAGGCGGTATTATtc                                                                                                                                                <  1:823413/251‑1 (MQ=255)                                                                                               cGGCTAGTGCCAAAGCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGAATTTCCGGTAATGATACTGAGGCGGTATTATtc                                                                                                                                                <  1:894239/251‑1 (MQ=255)                                                                                                  cTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGAATTTCCGGTAATGATACTGAGGCGGTATTATtctc                                                                                                                                              <  1:367573/250‑1 (MQ=255)                                                                                                     gtgcCAAATCGTGACCATCAATTAAGACTTGCCCCTGATCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGAATTTCCGGTAATGATACTGAGGCGGTATTATTCTCTGTa                                                                                                                                          >  1:694445/3‑251 (MQ=255)                                                                                                       gcCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGAATTTCCGGTAATGATACTGAGGCGGTATTATTCTCTGTAgg                                                                                                                                        >  1:86697/1‑251 (MQ=255)                                                                                                        ccAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGAATTTCCGGTAATGATACTGAGGCGGTATTATTCTCTGTAgg                                                                                                                                        <  1:562328/250‑1 (MQ=255)                                                                                                         cAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGAATTTCCGGTAATGATACTGAGGCGGTATTATTCTCTGTAGGCg                                                                                                                                      <  1:545294/251‑1 (MQ=255)                                                                                                            aTCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAat                                                                                                                                                                                                          >  1:520585/1‑180 (MQ=255)                                                                                                                       cAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGAATTTCCGGTAATGATACTGAGGCGGtat                                                                                                                                                     <  1:645786/222‑1 (MQ=255)                                                                                                                       cAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGAATTTCCGGTAATGATACTGAGGCGGTATTATTCTCTGTAGGCGAATTAAGCACATCa                                                                                                                        >  1:85100/1‑251 (MQ=255)                                                                                                                        aaTTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGAATTTCCGGTAATGATACTGAGGCGGTATTATTCTCTGTAGGCGAATTAAGCACATCa                                                                                                                        >  1:410468/1‑250 (MQ=255)                                                                                                                         aTTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGAATTTCCGGTAATGATACTGAGGCGGTATTATTCTCTGTAGGCGAATTAAGCACATCAcc                                                                                                                      <  1:537202/251‑1 (MQ=255)                                                                                                                             aGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGAATTTCCGGTAATGATACTGAGGCGGTATTATTCTCTGTAGGCGAATTAAGcac                                                                                                                            >  1:208524/1‑241 (MQ=255)                                                                                                                                       cTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGAATTTCCGGTAATGATACTGAGGCGGTATTATTCTCTGTAGGCGAATTAAGCACATCACCTAAGCGAGTAACAg                                                                                                        <  1:624685/251‑1 (MQ=255)                                                                                                                                        tGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGAATTTCCGGTAATGATACTGAGGCGGTATTATTCTCTGTAGGCGAATTAAGCACATCACCTAAGCGAGTAACAGa                                                                                                       <  1:519618/251‑1 (MQ=255)                                                                                                                                             cGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGAATTTCCGGTAATGATACTGAGGCGGTATTATTCTCTGTAGGCGAATTAAGCACATCACCTAAGCGAGTAACAGAAATAc                                                                                                  <  1:150814/251‑1 (MQ=255)                                                                                                                                                          cGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGAATTTCCGGTAATGATACTGAGGCGGTATTATTCTCTGTAGGCGAATTAAGCACATCACCTAAGCGAGTAACAGAAATACCGACCTGTTGGaa                                                                                     >  1:618147/1‑251 (MQ=255)                                                                                                                                                                    gTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGAATTTCCGGTAATGATACTGAGGCGGTATTATtc                                                                                                                                                <  1:902094/182‑1 (MQ=255)                                                                                                                                                                    gTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGAATTTCCGGTAATGATACTGAGGCGGTATTATTCTCTGTAGGCGAATTAAGCACATCACCTAAGCGAGTAACAGAAATACCGACCTGTTGGAAATCTTGCCAg                                                                           >  1:485925/1‑251 (MQ=255)                                                                                                                                                                     tttAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGAATTTCCGGTAATGATACTGAGGCGGTATTATTCTCTGTAGGCGAATTAAGCACATCAcctaag                                                                                                                  <  1:725778/211‑5 (MQ=255)                                                                                                                                                                                       tcccGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAgggg                                                                                                                                                                                                                                   <  1:420778/79‑1 (MQ=255)                                                                                                                                                                                                        caacaaTACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGAATTTCCGGTAATGATACTGAGGCGGTATTATTCTCTGTAGGCGAATTAAGCACATCACCTAAGCGAGTAACAGAAATACCGACCTGTTGGAAATCTTGCCAGATCTGGGCTAAACGAATCACGGGGCTAATAATTTGt                                       >  1:324998/1‑251 (MQ=255)                                                                                                                                                                                                             aTACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGAATTTCCGGTAATGATACTGAGGCGGTATTATTTTCTGTAGGCGAATTAAGCACATCACCTAAGCGAGTAACAGAAATACCGACCTGTTGGAAATCTTGCCAGATCTGGGCTAAACGAATCACGGGGCTAATAATTTGTCCCg                                   >  1:382408/1‑250 (MQ=255)                                                                                                                                                                                                               aCCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGAATTTCCGGTAATGATACTGAGGCGGTATTATTCTCTGTAGGCGAATTAAGCACATCACCTAAGCGAGTAACAGAAATACCGACCTGTTGGAAATCTTGCCAGATCTGGGCTAAACGAATCACGGGGCTAATAATTTGTCCCGCaa                                >  1:48820/1‑251 (MQ=255)                                                                                                                                                                                                               aCCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGAATTTCCGGTAATGATACTGAGGCGGTATTATTCTCTGTAGGCGAATTAAGCACATCACCTAAGCGAGTAACAGAAATACCGACCTGTTGGAAATCTTGCCAGATCTGGGCTAAACGAATCACGGGGCTAATAATTTGTCCCGCaa                                >  1:129395/1‑251 (MQ=255)                                                                                                                                                                                                                       cTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGAATTTCCGGTAATGATACTGAGGCGGTATTATTCTCTGTAGGCGAATTAAGCACATCACCTAAGCGAGTAACAGAAATAc                                                                                                  >  1:678962/1‑177 (MQ=255)                                                                                                                                                                                                                          ctcCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGAATTTCCGGTAATGATACTGAGGCGGTATTATtc                                                                                                                                                >  1:849352/1‑128 (MQ=255)                                                                                                                                                                                                                           tcCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGAATTTCCGGTAATGATACTGAGGCGGTATTATTCTCTGTAGGCGAATTAAGCACATCACCTAAGCGAGTAACAGAAATACCGACCTGTTGGAAATCTTGCCCGATCTGGGCTAAACGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATg                    >  1:229599/1‑251 (MQ=255)                                                                                                                                                                                                                                     atatCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGAATTTCCGGTAATGATAc                                                                                                                                                                <  1:169176/101‑1 (MQ=255)                                                                                                                                                                                                                                     atatCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGAATTTCCGGTAATGATACTGAGGCGGTATTATTCTCTGTAGGCGAATTAAGCACATCACCTAAGCGAGTAACAGAAATACCGACCTGTTGGAAATCTTGCCAGATCTGGGCTAAACGAATCACGGGGCTaataat                                           >  1:470451/1‑218 (MQ=255)                                                                                                                                                                                                                                       atCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGAATTTCCGGTAATGATACTGAGGCGGTATTATTCTCTGTAGGCGAATTAAGCACATCACCTAAGCGAGTAACAGAAATAc                                                                                                  <  1:399275/161‑1 (MQ=255)                                                                                                                                                                                                                                       atCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGAATTTCCGGTAATGATACTGAGGCGGTATTATTCTCTGTAGGCGAATTAAGCACATCACCTAAGCGAGTAACAGAAATAc                                                                                                  >  1:20300/1‑161 (MQ=255)                                                                                                                                                                                                                                           gaGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGAATTTCCGGTAATGATACTGAgg                                                                                                                                                           <  1:230610/99‑1 (MQ=255)                                                                                                                                                                                                                                           aaGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGAATTTCCGGTAATGATACTGAGGCGGTATTATTCTCTGTAGGCGAATTAAGCACATCACCTAAGCGAGTAACAGAAATACCGACCTGTTGGAAATCTTGCCAGATCTGGGCTAAACGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATg                    <  1:10419/235‑1 (MQ=255)                                                                                                                                                                                                                                           aaGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGAATTTCCGGTAATGATACTGAGGCGGTATTATTCTCTGTAGGCGAATTAAGCACATCACCTAAGCGAGTAACAGAAATACCGACCTGTTGGAAATCTTGCCAGATCTGGGCTAAACGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGc            >  1:855018/1‑243 (MQ=255)                                                                                                                                                                                                                                           aaGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGAATTTCCGGTAATGATACTGAGGCGGTATTATTCTCTGTAGGCGAATTAAGCACATCACCTAAGCGAGTAACAGAAATACCGACCTGTTGGAAATCTTGCCAGATCTGGGCTAAACGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAAt    >  1:597701/1‑251 (MQ=255)                                                                                                                                                                                                                                           aaGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGAATTTCCGGTAATGATACTGAGGCGGTATTATTCTCTGTAGGCGAATTAAGCACATCACCTAAGCGAGTAACAGAAATACCGACCTGTTGGAAATCTTGCCAGATCTGGGCTAAACGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAAt    >  1:119857/1‑251 (MQ=255)                                                                                                                                                                                                                                            aGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCAccc                                                                                                                                                                                    <  1:194930/74‑1 (MQ=255)                                                                                                                                                                                                                                            aGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGAATTTCCGGTAATGATACTGAGGCGGTATTATTCTCTGTAGGCg                                                                                                                                      <  1:750308/120‑1 (MQ=255)                                                                                                                                                                                                                                            aGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGAATTTCCGGTAATGATACTGAGGCGGTATTATTCTCTGTAGGCGAATTAAGCACATCACCTAAGCGAGTAACAGAAATACCGACCTGTTGGAAATCTTGCCAGATCTGGGCTAAACGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTa                        <  1:488548/230‑1 (MQ=255)                                                                                                                                                                                                                                            aGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGAATTTCCGGTAATGATACTGAGGCGGTATTATTCTCTGTAGGCGAATTAAGCACATCACCTAAGCGAGTAACAGAAATACCGACCTGTTGGAAATCTTGCCAGATCTGGGCTAAACGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATg                    <  1:802467/234‑1 (MQ=255)                                                                                                                                                                                                                                             gTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGAATTTCCGGTAATGATACTGAGGCGGTATTATtc                                                                                                                                                <  1:49356/109‑1 (MQ=255)                                                                                                                                                                                                                                             gTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGAATTTCCGGTAATGATACTGAGGCGGTATTATTCTCTGTAGGCGAATTAAGCACATCACCTAAGCGAGTAACAGAAATACCGACCTGTTGGAAAt                                                                                   >  1:858064/1‑170 (MQ=255)                                                                                                                                                                                                                                             gTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGAATTTCCGGTAATGATACTGAGGCGGTATTATTCTCTGTAGGCGAATTAAGCACATCACCTAAGCGAGTAACAGAAATACCGACCTGTTGGAAATCTTGCCAGATCTGGGCTAAACGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATa   >  1:896051/1‑250 (MQ=255)                                                                                                                                                                                                                                              ttAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGAATTTCCGGTAATGATACTGAGGCGGTATTATTCTCTGTAGGCGAATTAAGCACATCACCTAAGCGAGTAACAGAAATACCGACCTGTTGGAAATCTTGCCAGATCTGGGCTAAACGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATAc  <  1:145476/250‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                    GGATTGGTTAACGCAATATTCTCTCTGATACTTCGATTAAGCAACACATTATCTTGCAAGACGACACCGACTTGCCGGCGCAACCAGTTTGGATCGGCTAGCGCCAAATCGTGACCGTCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATGAGTTTAGTAAGCGTACTTTTCCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATATTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGGATTTCCGGTAATGATACTGAGGCGGTATTATTCTCTGTAGGCGAATTAAGCACATCACCCAAACGAGTAACAGAAATACCGACCTGTTGGAAATCTTGCCAGATCTGGGCTAAACGAATCACAGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATAC  >  1/1819253‑1819739

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑