Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,819,435	C→T	intergenic (+37535/+15949)	unknown → / ← unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,819,435	0	C	T	100.0%	194.8 / NA	65	intergenic (+37535/+15949)	unknown/unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (30/35);  total (30/35)

ATCGTGCGCTCCCGCAAGTTTTGCCGCGGCAATAACCTTTTCCATTGGCATTCCCGGATTGGTTAACGCAATATTCTCTCTGATACTTCGATTAAGCAACACATTATCTTGCAAGACGACACCGACTTGCCGGCGCAACCAGTTTGGATCGGCTAGCGCCAAATCGTGACCGTCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATGAGTTTAGTAAGCGTACTTTTCCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATATTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGGATTTCCGGTAATGATACTGAGGCGGTATTATTCTCTGTAGGCGAATTAAGCACATCACCCAAACGAGTAACAGAAATACCGACCTGTTGGAAATCTTGCCAG  >  1/1819198‑1819666                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                        aTCGTGCGCGCCCGCAAGTTTTGCCGCGGCAATAACCTTTTCCATTGACATTCCCGGATTGGTTAACGCAATATTCTCTCTGATACTTCGATTAAGCAACACATTATCTTGCAGGACGACACCTACTTGCCGGCGTAACCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAAc                                                                                                                                                                                                                            <  1:764428/251‑1 (MQ=255)             cGCAAGTTTTGCCGCGGCAATAACCTTTTCCATTGACATTCCCGGATTGGTTAACGCAATATTCTCTCTGATACTTCGATTAAGCAACACATTATCTTGCAGGACGACACCTACTTGCCGGCGTAACCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATAc                                                                                                                                                                                                                <  1:761872/251‑1 (MQ=255)             cGCAAGTTTTGCCGCGGCAATAACCTTTTCCATTGACATTCCCGGATTGGTTAACGCAATATTCTCTCTGATACTTCGATTAAGCAACACATTATCTTGCAGGACGACACCTACTTGCCGGCGTAACCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATAc                                                                                                                                                                                                                <  1:15789/251‑1 (MQ=255)                  gTTTTGCCGCGGCAATAACCTTTTCCATTGACATTCCCGGATTGGTTAACGCAATATTCTCTCTGATACTTCGATTAAGCAACACATTATCTTGCAGGACGACACCTACTTGCCGGCGTAACCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAAt                                                                                                                                                                                                            >  1:280175/1‑250 (MQ=255)                           cgGCAATAACCTTTTCCATTGACATTCCCGGATTGGTTAACGCAATATTCTCTCTGATACTTCGATTAAGCAACACATTATCTTGCAGGACGACACCTACTTGCCGGCGTAACCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTccc                                                                                                                                                                                                  <  1:892541/251‑1 (MQ=255)                             gCAATAACCTTTTCCATTGACATTCCCGGATTGGTTAACGCAATATTCTCTCTGATACTTCGATTAAGCAACACATTATCTTGCAGGACGACACCTACTTGCCGGCGTAACCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTcc                                                                                                                                                                                                   >  1:284966/1‑248 (MQ=255)                               aaTAACCTTTTCCATTGACATTCCCGGATTGGTTAACGCAATATTCTCTCTGATACTTCGATTAAGCAACACATTATCTTGCAGGACGACACCTACTTGCCGGCGTAACCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCctgac                                                                                                                                                                                              <  1:489981/251‑1 (MQ=255)                                   aCCTTTTCCATTGACATTCCCGGATTGGTTAACGCAATATTCTCTCTGATACTTCGATTAAGCAACACATTATCTTGCAGGACGACACCTACTTGCCGGCGTAACCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATAc                                                                                                                                                                                                                >  1:87954/1‑229 (MQ=255)                                           cATTGACATTCCCGGATTGGTTAACGCAATATTCTCTCTGATACTTCGATTAAGCAACACATTATCTTGCAGGACGACACCTACTTGCCGGCGTAACCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCctgac                                                                                                                                                                                              <  1:776606/239‑1 (MQ=255)                                            aTTGACATTCCCGGATTGGTTAACGCAATATTCTCTCTGATACTTCGATTAAGCAACACATTATCTTGCAGGACGACACCTACTTGCCGGCGTAACCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGtt                                                                                                                                                                                 <  1:785387/251‑1 (MQ=255)                                                 cATTCCCGGATTGGTTAACGCAATATTCTCTCTGATACTTCGATTAAGCAACACATTATCTTGCAGGACGACACCTACTTGCCGGCGTAACCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAatac                                                                                                                                                                            <  1:174856/251‑2 (MQ=255)                                                    tCCCGGATTGGTTAACGCAATATTCTCTCTGATACTTCGATTAAGCAACACATTATCTTGCAGGACGACACCTACTTGCCGGCGTAACCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACcaaca                                                                                                                                                                                                                    <  1:682032/208‑1 (MQ=255)                                                        ggATTGGTTAACGCAATATTCTCTCTGATACTTCGATTAAGCAACACATTATCTTGCAGGACGACACCTACTTGCCGGCGTAACCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTaa                                                                                                                                                                     >  1:573076/1‑251 (MQ=255)                                                                  aCGCAATATTCTCTCTGATACTTCGATTAAGCAACACATTATCTTGCAGGACGACACCTACTTGCCGGCGTAACCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAatac                                                                                                                                                                            <  1:460415/234‑2 (MQ=255)                                                                  aCGCAATATTCTCTCTGATACTTCGATTAAGCAACACATTATCTTGCAGGACGACACCTACTTGCCGGCGTAACCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAggg                                                                                                                                                           >  1:146314/1‑251 (MQ=255)                                                                   cGCAATATTCTCTCTGATACTTCGATTAAGCAACACATTATCTTGCAGGACGACACCTACTTGCCGGCGTAACCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAgggg                                                                                                                                                          <  1:380542/251‑1 (MQ=255)                                                                          ttCTCTCTGATACTTCGATTAAGCAACACATTATCTTGCAGGACGACACCTACTTGCCGGCGTAACCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCgg                                                                                                                                                   <  1:244782/251‑1 (MQ=255)                                                                          ttCTCTCTGATACTTCGATTAAGCAACACATTATCTTGCAGGACGACACCTACTTGCCGGCGTAACCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCgg                                                                                                                                                   <  1:111098/251‑1 (MQ=255)                                                                              ctctGATACTTCGATTAAGCAACACATTATCTTGCAGGACGACACCTACTTGCCGGCGTAACCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTta                                                                                                                                               >  1:198995/1‑251 (MQ=255)                                                                                              aaGCAACACATTATCTTGCAGGACGACACCTACTTGCCGGCGTAACCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCa                                                                                                                                                                                                              >  1:734794/1‑172 (MQ=255)                                                                                                   acacATTATCTTGCAGGACGACACCTACTTGCCGGCGTAACCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAAc                                                                                                                                                                                                                            >  1:33385/1‑153 (MQ=255)                                                                                                   acacATTATCTTGCAGGACGACACCTACTTGCCGGCGTAACCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTa                                                                                                                          >  1:129552/1‑251 (MQ=255)                                                                                                    cacaTTATCTTGCAGGACGACACCTACTTGCCGGCGTAACCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAAt                                                                                                                                                                                                                  >  1:249387/1‑162 (MQ=255)                                                                                                        ttATCTTGCAGGACGACACCTACTTGCCGGCGTAACCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTaaaa                                                                                                                       >  1:414736/1‑249 (MQ=255)                                                                                                          aTCTTGCAGGACGACACCTACTTGCCGGCGTAACCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATAcc                                                                                                                                                                                                               <  1:493244/159‑1 (MQ=255)                                                                                                          aTCTTGCAGGACGACACCTACTTGCCGGCGTAACCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGatat                                                                                                                                                                                        <  1:775951/182‑1 (MQ=255)                                                                                                          aTCTTGCAGGACGACACCTACTTGCCGGCGTAACCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTaa                                                                                                                   >  1:814257/1‑251 (MQ=255)                                                                                                                  ggacgacACCTACTTGCCGGCGTAACCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCAcc                                                                                                            >  1:647313/2‑250 (MQ=255)                                                                                                                    acgacACCTACTTGCCGGCGTAACCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAgggg                                                                                                                                                          >  1:183568/1‑202 (MQ=255)                                                                                                                          cctaCTTGCCGGCGTAACCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTcc                                                                                                                                                                                                   <  1:566233/152‑1 (MQ=255)                                                                                                                                      cgtaaCCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGAATTTCCGGTAATGAt                                                                                         >  1:756227/4‑249 (MQ=255)                                                                                                                                        taaCCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGAATTTCCGGTAATGATACTg                                                                                     <  1:387956/250‑1 (MQ=255)                                                                                                                                         aaCCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAAtctc                                                                                                               <  1:745239/224‑1 (MQ=255)                                                                                                                                         aaCCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGAATTTCCGGTAATGATACTGa                                                                                    >  1:636046/1‑251 (MQ=255)                                                                                                                                             aGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGAATTTCCGGTAATGATACTGAGGCg                                                                                >  1:735619/1‑251 (MQ=255)                                                                                                                                              gTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCctgac                                                                                                                                                                                              >  1:103689/1‑140 (MQ=255)                                                                                                                                              gTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGAATTTCCGGTAATGATACTGAGGCgg                                                                               >  1:540391/1‑251 (MQ=255)                                                                                                                                              gTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGAATTTCCGGTAATGATACTGAGGCgg                                                                               >  1:684688/1‑251 (MQ=255)                                                                                                                                                    aTCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGAATTTCCGGTAATGATACTGAGGCGGtattat                                                                         <  1:560237/251‑1 (MQ=255)                                                                                                                                                      cGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGAATTTCCGGTAATGATACTGAGGCGGTATTATtc                                                                       <  1:247570/251‑1 (MQ=255)                                                                                                                                                      cGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGAATTTCCGGTAATGATACTGAGGCGGTATTATtc                                                                       <  1:61694/251‑1 (MQ=255)                                                                                                                                                      cGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGAATTTCCGGTAATGATACTGAGGCGGTATTATtc                                                                       <  1:534675/251‑1 (MQ=255)                                                                                                                                                      cGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATACCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGAATTTCCGGTAATGATACTGAGGCGGTATTATtc                                                                       <  1:823413/251‑1 (MQ=255)                                                                                                                                                      cGGCTAGTGCCAAAGCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGAATTTCCGGTAATGATACTGAGGCGGTATTATtc                                                                       <  1:894239/251‑1 (MQ=255)                                                                                                                                                         cTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGAATTTCCGGTAATGATACTGAGGCGGTATTATtctc                                                                     <  1:367573/250‑1 (MQ=255)                                                                                                                                                            gtgcCAAATCGTGACCATCAATTAAGACTTGCCCCTGATCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGAATTTCCGGTAATGATACTGAGGCGGTATTATTCTCTGTa                                                                 >  1:694445/3‑251 (MQ=255)                                                                                                                                                              gcCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGAATTTCCGGTAATGATACTGAGGCGGTATTATTCTCTGTAgg                                                               >  1:86697/1‑251 (MQ=255)                                                                                                                                                               ccAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGAATTTCCGGTAATGATACTGAGGCGGTATTATTCTCTGTAgg                                                               <  1:562328/250‑1 (MQ=255)                                                                                                                                                                cAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGAATTTCCGGTAATGATACTGAGGCGGTATTATTCTCTGTAGGCg                                                             <  1:545294/251‑1 (MQ=255)                                                                                                                                                                   aTCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAat                                                                                                                                 >  1:520585/1‑180 (MQ=255)                                                                                                                                                                              cAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGAATTTCCGGTAATGATACTGAGGCGGtat                                                                            <  1:645786/222‑1 (MQ=255)                                                                                                                                                                              cAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGAATTTCCGGTAATGATACTGAGGCGGTATTATTCTCTGTAGGCGAATTAAGCACATCa                                               >  1:85100/1‑251 (MQ=255)                                                                                                                                                                               aaTTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGAATTTCCGGTAATGATACTGAGGCGGTATTATTCTCTGTAGGCGAATTAAGCACATCa                                               >  1:410468/1‑250 (MQ=255)                                                                                                                                                                                aTTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATc                                                                                                                                                                                                           >  1:250836/1‑93 (MQ=255)                                                                                                                                                                                aTTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTccc                                                                                                                                                                                                  <  1:411544/102‑1 (MQ=255)                                                                                                                                                                                aTTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGAATTTCCGGTAATGATACTGAGGCGGTATTATTCTCTGTAGGCGAATTAAGCACATCAcc                                             <  1:537202/251‑1 (MQ=255)                                                                                                                                                                                    aGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGAATTTCCGGTAATGATACTGAGGCGGTATTATTCTCTGTAGGCGAATTAAGcac                                                   >  1:208524/1‑241 (MQ=255)                                                                                                                                                                                              cTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGAATTTCCGGTAATGATACTGAGGCGGTATTATTCTCTGTAGGCGAATTAAGCACATCACCTAAGCGAGTAACAg                               <  1:624685/251‑1 (MQ=255)                                                                                                                                                                                               tGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGAATTTCCGGTAATGATACTGAGGCGGTATTATTCTCTGTAGGCGAATTAAGCACATCACCTAAGCGAGTAACAGa                              <  1:519618/251‑1 (MQ=255)                                                                                                                                                                                                    cGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGAATTTCCGGTAATGATACTGAGGCGGTATTATTCTCTGTAGGCGAATTAAGCACATCACCTAAGCGAGTAACAGAAATAc                         <  1:150814/251‑1 (MQ=255)                                                                                                                                                                                                        atatAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCctg                                                                                                                                                                                                >  1:676185/1‑80 (MQ=255)                                                                                                                                                                                                                 cGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGAATTTCCGGTAATGATACTGAGGCGGTATTATTCTCTGTAGGCGAATTAAGCACATCACCTAAGCGAGTAACAGAAATACCGACCTGTTGGaa            >  1:618147/1‑251 (MQ=255)                                                                                                                                                                                                                           gTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGAATTTCCGGTAATGATACTGAGGCGGTATTATtc                                                                       <  1:902094/182‑1 (MQ=255)                                                                                                                                                                                                                           gTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGAATTTCCGGTAATGATACTGAGGCGGTATTATTCTCTGTAGGCGAATTAAGCACATCACCTAAGCGAGTAACAGAAATACCGACCTGTTGGAAATCTTGCCAg  >  1:485925/1‑251 (MQ=255)                                                                                                                                                                                                                            tttAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGAATTTCCGGTAATGATACTGAGGCGGTATTATTCTCTGTAGGCGAATTAAGCACATCAcctaag                                         <  1:725778/211‑5 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                        ATCGTGCGCTCCCGCAAGTTTTGCCGCGGCAATAACCTTTTCCATTGGCATTCCCGGATTGGTTAACGCAATATTCTCTCTGATACTTCGATTAAGCAACACATTATCTTGCAAGACGACACCGACTTGCCGGCGCAACCAGTTTGGATCGGCTAGCGCCAAATCGTGACCGTCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATGAGTTTAGTAAGCGTACTTTTCCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATATTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGGATTTCCGGTAATGATACTGAGGCGGTATTATTCTCTGTAGGCGAATTAAGCACATCACCCAAACGAGTAACAGAAATACCGACCTGTTGGAAATCTTGCCAG  >  1/1819198‑1819666

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑