Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,819,414	G→A	intergenic (+37514/+15970)	unknown → / ← unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,819,414	0	G	A	100.0%	206.0 / NA	72	intergenic (+37514/+15970)	unknown/unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (34/38);  total (34/38)

TGTTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCTCCCGCAAGTTTTGCCGCGGCAATAACCTTTTCCATTGGCATTCCCGGATTGGTTAACGCAATATTCTCTCTGATACTTCGATTAAGCAACACATTATCTTGCAAGACGACACCGACTTGCCGGCGCAACCAGTTTGGATCGGCTAGCGCCAAATCGTGACCGTCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATGAGTTTAGTAAGCGTACTTTTCCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATATTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGGATTTCCGGTAATGATACTGAGGCGGTATTATTCTCTGTAGGCGAATTAAGCACATCACCCAAACGAGTAACAGAAATACCGACCTGTTGGAA  >  1/1819167‑1819656                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                   tgtTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCGCCCGCAAGTTTTGCCGCGGCAATAACCTTTTCCATTGACATTCCCGGATTGGTTAACGCAATATTCTCTCTGATACTTCGATTAAGCAACACATTATCTTGCAGGACGACACCTACTTGCCGGCGTAACCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGt                                                                                                                                                                                                                                                 >  1:317725/1‑251 (MQ=255)  gtTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCGCCCGCAAGTTTTGCCGCTGCAATAACCTTTTCCATTGACATTCCCGGATTGGTTAACGCAATATTCTCTCTGATACTTCGATTAAGCAACACATTATCTTGCAGGACGACACCTACTTGCCGGCGTAACCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGCATATAAAAACGTTGGATAAGtt                                                                                                                                                                                                                                                >  1:218832/1‑251 (MQ=255)  gtTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCGCCCGCAAGTTTTGCCGCGGCAATAACCTTTTCCATTGACATTCCCGGATTGGTTAACGCAATATTCTCTCTGATACTTCGATTAAGCAACACATTATCTTGCAGGACGACACCTACTTGCCGGCGTAACCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGtt                                                                                                                                                                                                                                                >  1:264155/1‑251 (MQ=255)  gtTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCGCCCACAAGTTTTGCCGCGGCAATAACCTTTTCCATTGACATTCCCGGATTGGTTAACGCAATATTCTCTCTGATACTTCGATTAAGCAACACATTATCTTGCAGGACGACACCTACTTGCCGGCGTAACCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGtt                                                                                                                                                                                                                                                >  1:163117/1‑251 (MQ=255)            tctcTTAATTCAGAAATAAAATCGTGCGCGCCCGCAAGTTTTGCCGCGGCAATAACCTTTTCCATTGACATTCCCGGATTGGTTAACGCAATATTCTCTCTGATACTTCGATTAAGCAACACATTATCTTGCAGGACGACACCTACTTGCCGGCGTAACCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGt                                                                                                                                                                                                                                      >  1:297873/1‑251 (MQ=255)                                aTCGTGCGCGCCCGCAAGTTTTGCCGCGGCAATAACCTTTTCCATTGACATTCCCGGATTGGTTAACGCAATATTCTCTCTGATACTTCGATTAAGCAACACATTATCTTGCAGGACGACACCTACTTGCCGGCGTAACCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAAc                                                                                                                                                                                                                  <  1:764428/251‑1 (MQ=255)                                            cGCAAGTTTTGCCGCGGCAATAACCTTTTCCATTGACATTCCCGGATTGGTTAACGCAATATTCTCTCTGATACTTCGATTAAGCAACACATTATCTTGCAGGACGACACCTACTTGCCGGCGTAACCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATAc                                                                                                                                                                                                      <  1:761872/251‑1 (MQ=255)                                            cGCAAGTTTTGCCGCGGCAATAACCTTTTCCATTGACATTCCCGGATTGGTTAACGCAATATTCTCTCTGATACTTCGATTAAGCAACACATTATCTTGCAGGACGACACCTACTTGCCGGCGTAACCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATAc                                                                                                                                                                                                      <  1:15789/251‑1 (MQ=255)                                                 gTTTTGCCGCGGCAATAACCTTTTCCATTGACATTCCCGGATTGGTTAACGCAATATTCTCTCTGATACTTCGATTAAGCAACACATTATCTTGCAGGACGACACCTACTTGCCGGCGTAACCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAAt                                                                                                                                                                                                  >  1:280175/1‑250 (MQ=255)                                                          cgGCAATAACCTTTTCCATTGACATTCCCGGATTGGTTAACGCAATATTCTCTCTGATACTTCGATTAAGCAACACATTATCTTGCAGGACGACACCTACTTGCCGGCGTAACCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTccc                                                                                                                                                                                        <  1:892541/251‑1 (MQ=255)                                                            gCAATAACCTTTTCCATTGACATTCCCGGATTGGTTAACGCAATATTCTCTCTGATACTTCGATTAAGCAACACATTATCTTGCAGGACGACACCTACTTGCCGGCGTAACCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTaa                                                                                                                                                                                                                                          <  1:713443/199‑1 (MQ=255)                                                            gCAATAACCTTTTCCATTGACATTCCCGGATTGGTTAACGCAATATTCTCTCTGATACTTCGATTAAGCAACACATTATCTTGCAGGACGACACCTACTTGCCGGCGTAACCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTcc                                                                                                                                                                                         >  1:284966/1‑248 (MQ=255)                                                              aaTAACCTTTTCCATTGACATTCCCGGATTGGTTAACGCAATATTCTCTCTGATACTTCGATTAAGCAACACATTATCTTGCAGGACGACACCTACTTGCCGGCGTAACCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCctgac                                                                                                                                                                                    <  1:489981/251‑1 (MQ=255)                                                                  aCCTTTTCCATTGACATTCCCGGATTGGTTAACGCAATATTCTCTCTGATACTTCGATTAAGCAACACATTATCTTGCAGGACGACACCTACTTGCCGGCGTAACCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATAc                                                                                                                                                                                                      >  1:87954/1‑229 (MQ=255)                                                                          cATTGACATTCCCGGATTGGTTAACGCAATATTCTCTCTGATACTTCGATTAAGCAACACATTATCTTGCAGGACGACACCTACTTGCCGGCGTAACCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCctgac                                                                                                                                                                                    <  1:776606/239‑1 (MQ=255)                                                                           aTTGACATTCCCGGATTGGTTAACGCAATATTCTCTCTGATACTTCGATTAAGCAACACATTATCTTGCAGGACGACACCTACTTGCCGGCGTAACCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGtt                                                                                                                                                                       <  1:785387/251‑1 (MQ=255)                                                                                cATTCCCGGATTGGTTAACGCAATATTCTCTCTGATACTTCGATTAAGCAACACATTATCTTGCAGGACGACACCTACTTGCCGGCGTAACCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAatac                                                                                                                                                                  <  1:174856/251‑2 (MQ=255)                                                                                   tCCCGGATTGGTTAACGCAATATTCTCTCTGATACTTCGATTAAGCAACACATTATCTTGCAGGACGACACCTACTTGCCGGCGTAACCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACcaaca                                                                                                                                                                                                          <  1:682032/208‑1 (MQ=255)                                                                                       ggATTGGTTAACGCAATATTCTCTCTGATACTTCGATTAAGCAACACATTATCTTGCAGGACGACACCTACTTGCCGGCGTAACCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTaa                                                                                                                                                           >  1:573076/1‑251 (MQ=255)                                                                                                 aCGCAATATTCTCTCTGATACTTCGATTAAGCAACACATTATCTTGCAGGACGACACCTACTTGCCGGCGTAACCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAatac                                                                                                                                                                  <  1:460415/234‑2 (MQ=255)                                                                                                 aCGCAATATTCTCTCTGATACTTCGATTAAGCAACACATTATCTTGCAGGACGACACCTACTTGCCGGCGTAACCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAggg                                                                                                                                                 >  1:146314/1‑251 (MQ=255)                                                                                                  cGCAATATTCTCTCTGATACTTCGATTAAGCAACACATTATCTTGCAGGACGACACCTACTTGCCGGCGTAACCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTa                                                                                                                                                                                                                                           <  1:606997/160‑1 (MQ=255)                                                                                                  cGCAATATTCTCTCTGATACTTCGATTAAGCAACACATTATCTTGCAGGACGACACCTACTTGCCGGCGTAACCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAgggg                                                                                                                                                <  1:380542/251‑1 (MQ=255)                                                                                                         ttCTCTCTGATACTTCGATTAAGCAACACATTATCTTGCAGGACGACACCTACTTGCCGGCGTAACCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCgg                                                                                                                                         <  1:111098/251‑1 (MQ=255)                                                                                                         ttCTCTCTGATACTTCGATTAAGCAACACATTATCTTGCAGGACGACACCTACTTGCCGGCGTAACCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCgg                                                                                                                                         <  1:244782/251‑1 (MQ=255)                                                                                                          tctctcTGATACTTCGATTAAGCAACACATTATCTTGCAGGACGACACCTACTTGCCGGCGTAACCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACttt                                                                                                                                                                                                                                 <  1:163347/162‑1 (MQ=255)                                                                                                             ctctGATACTTCGATTAAGCAACACATTATCTTGCAGGACGACACCTACTTGCCGGCGTAACCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTa                                                                                                                                                                                                                                           <  1:533600/149‑1 (MQ=255)                                                                                                             ctctGATACTTCGATTAAGCAACACATTATCTTGCAGGACGACACCTACTTGCCGGCGTAACCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTta                                                                                                                                     >  1:198995/1‑251 (MQ=255)                                                                                                                             aaGCAACACATTATCTTGCAGGACGACACCTACTTGCCGGCGTAACCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCa                                                                                                                                                                                                    >  1:734794/1‑172 (MQ=255)                                                                                                                                  acacATTATCTTGCAGGACGACACCTACTTGCCGGCGTAACCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAAc                                                                                                                                                                                                                  >  1:33385/1‑153 (MQ=255)                                                                                                                                  acacATTATCTTGCAGGACGACACCTACTTGCCGGCGTAACCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTa                                                                                                                >  1:129552/1‑251 (MQ=255)                                                                                                                                   cacaTTATCTTGCAGGACGACACCTACTTGCCGGCGTAACCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAAt                                                                                                                                                                                                        >  1:249387/1‑162 (MQ=255)                                                                                                                                       ttATCTTGCAGGACGACACCTACTTGCCGGCGTAACCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTaaaa                                                                                                             >  1:414736/1‑249 (MQ=255)                                                                                                                                         aTCTTGCAGGACGACACCTACTTGCCGGCGTAACCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATAcc                                                                                                                                                                                                     <  1:493244/159‑1 (MQ=255)                                                                                                                                         aTCTTGCAGGACGACACCTACTTGCCGGCGTAACCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGatat                                                                                                                                                                              <  1:775951/182‑1 (MQ=255)                                                                                                                                         aTCTTGCAGGACGACACCTACTTGCCGGCGTAACCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTaa                                                                                                         >  1:814257/1‑251 (MQ=255)                                                                                                                                                 ggacgacACCTACTTGCCGGCGTAACCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTa                                                                                                                                                                                                                                           <  1:629686/112‑1 (MQ=255)                                                                                                                                                 ggacgacACCTACTTGCCGGCGTAACCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCAcc                                                                                                  >  1:647313/2‑250 (MQ=255)                                                                                                                                                   acgacACCTACTTGCCGGCGTAACCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAgggg                                                                                                                                                >  1:183568/1‑202 (MQ=255)                                                                                                                                                         cctaCTTGCCGGCGTAACCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTcc                                                                                                                                                                                         <  1:566233/152‑1 (MQ=255)                                                                                                                                                                     cgtaaCCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGAATTTCCGGTAATGAt                                                                               >  1:756227/4‑249 (MQ=255)                                                                                                                                                                       taaCCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGAATTTCCGGTAATGATACTg                                                                           <  1:387956/250‑1 (MQ=255)                                                                                                                                                                        aaCCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAAtctc                                                                                                     <  1:745239/224‑1 (MQ=255)                                                                                                                                                                        aaCCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGAATTTCCGGTAATGATACTGa                                                                          >  1:636046/1‑251 (MQ=255)                                                                                                                                                                            aGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGAATTTCCGGTAATGATACTGAGGCg                                                                      >  1:735619/1‑251 (MQ=255)                                                                                                                                                                             gTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCctgac                                                                                                                                                                                    >  1:103689/1‑140 (MQ=255)                                                                                                                                                                             gTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGAATTTCCGGTAATGATACTGAGGCgg                                                                     >  1:684688/1‑251 (MQ=255)                                                                                                                                                                             gTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGAATTTCCGGTAATGATACTGAGGCgg                                                                     >  1:540391/1‑251 (MQ=255)                                                                                                                                                                                   aTCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGAATTTCCGGTAATGATACTGAGGCGGtattat                                                               <  1:560237/251‑1 (MQ=255)                                                                                                                                                                                     cGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGAATTTCCGGTAATGATACTGAGGCGGTATTATtc                                                             <  1:61694/251‑1 (MQ=255)                                                                                                                                                                                     cGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGAATTTCCGGTAATGATACTGAGGCGGTATTATtc                                                             <  1:534675/251‑1 (MQ=255)                                                                                                                                                                                     cGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGAATTTCCGGTAATGATACTGAGGCGGTATTATtc                                                             <  1:247570/251‑1 (MQ=255)                                                                                                                                                                                     cGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATACCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGAATTTCCGGTAATGATACTGAGGCGGTATTATtc                                                             <  1:823413/251‑1 (MQ=255)                                                                                                                                                                                     cGGCTAGTGCCAAAGCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGAATTTCCGGTAATGATACTGAGGCGGTATTATtc                                                             <  1:894239/251‑1 (MQ=255)                                                                                                                                                                                        cTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGAATTTCCGGTAATGATACTGAGGCGGTATTATtctc                                                           <  1:367573/250‑1 (MQ=255)                                                                                                                                                                                           gtgcCAAATCGTGACCATCAATTAAGACTTGCCCCTGATCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGAATTTCCGGTAATGATACTGAGGCGGTATTATTCTCTGTa                                                       >  1:694445/3‑251 (MQ=255)                                                                                                                                                                                             gcCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGAATTTCCGGTAATGATACTGAGGCGGTATTATTCTCTGTAgg                                                     >  1:86697/1‑251 (MQ=255)                                                                                                                                                                                              ccAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGAATTTCCGGTAATGATACTGAGGCGGTATTATTCTCTGTAgg                                                     <  1:562328/250‑1 (MQ=255)                                                                                                                                                                                               cAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGAATTTCCGGTAATGATACTGAGGCGGTATTATTCTCTGTAGGCg                                                   <  1:545294/251‑1 (MQ=255)                                                                                                                                                                                                  aTCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAat                                                                                                                       >  1:520585/1‑180 (MQ=255)                                                                                                                                                                                                             cAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGAATTTCCGGTAATGATACTGAGGCGGtat                                                                  <  1:645786/222‑1 (MQ=255)                                                                                                                                                                                                             cAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGAATTTCCGGTAATGATACTGAGGCGGTATTATTCTCTGTAGGCGAATTAAGCACATCa                                     >  1:85100/1‑251 (MQ=255)                                                                                                                                                                                                              aaTTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGAATTTCCGGTAATGATACTGAGGCGGTATTATTCTCTGTAGGCGAATTAAGCACATCa                                     >  1:410468/1‑250 (MQ=255)                                                                                                                                                                                                               aTTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATc                                                                                                                                                                                                 >  1:250836/1‑93 (MQ=255)                                                                                                                                                                                                               aTTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTccc                                                                                                                                                                                        <  1:411544/102‑1 (MQ=255)                                                                                                                                                                                                               aTTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGAATTTCCGGTAATGATACTGAGGCGGTATTATTCTCTGTAGGCGAATTAAGCACATCAcc                                   <  1:537202/251‑1 (MQ=255)                                                                                                                                                                                                                   aGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGAATTTCCGGTAATGATACTGAGGCGGTATTATTCTCTGTAGGCGAATTAAGcac                                         >  1:208524/1‑241 (MQ=255)                                                                                                                                                                                                                             cTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGAATTTCCGGTAATGATACTGAGGCGGTATTATTCTCTGTAGGCGAATTAAGCACATCACCTAAGCGAGTAACAg                     <  1:624685/251‑1 (MQ=255)                                                                                                                                                                                                                              tGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGAATTTCCGGTAATGATACTGAGGCGGTATTATTCTCTGTAGGCGAATTAAGCACATCACCTAAGCGAGTAACAGa                    <  1:519618/251‑1 (MQ=255)                                                                                                                                                                                                                                   cGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGAATTTCCGGTAATGATACTGAGGCGGTATTATTCTCTGTAGGCGAATTAAGCACATCACCTAAGCGAGTAACAGAAATAc               <  1:150814/251‑1 (MQ=255)                                                                                                                                                                                                                                       atatAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCctg                                                                                                                                                                                      >  1:676185/1‑80 (MQ=255)                                                                                                                                                                                                                                                cGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATACTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGAATTTCCGGTAATGATACTGAGGCGGTATTATTCTCTGTAGGCGAATTAAGCACATCACCTAAGCGAGTAACAGAAATACCGACCTGTTGGaa  >  1:618147/1‑251 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                   TGTTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCTCCCGCAAGTTTTGCCGCGGCAATAACCTTTTCCATTGGCATTCCCGGATTGGTTAACGCAATATTCTCTCTGATACTTCGATTAAGCAACACATTATCTTGCAAGACGACACCGACTTGCCGGCGCAACCAGTTTGGATCGGCTAGCGCCAAATCGTGACCGTCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATGAGTTTAGTAAGCGTACTTTTCCCCGAACCTGAACGACCAACAATACCAATCACTTCTCCCTGACTGATATCTAAGTTAATATTGTTTAAAATCATAGGGGAATCCGGTTTATAGCGGAACTTAATATTTCTAAAACTAATCTCACCCTGGATTTCCGGTAATGATACTGAGGCGGTATTATTCTCTGTAGGCGAATTAAGCACATCACCCAAACGAGTAACAGAAATACCGACCTGTTGGAA  >  1/1819167‑1819656

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑